E2F5 Antibody Cat. No.: 31-226
E2F5 Antibody
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human E2F5.
TESTED APPLICATIONS: ELISA, WB
E2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can APPLICATIONS: be used for detection of E2F5 by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate
PREDICTED MOLECULAR 38 kDa WEIGHT:
Properties
PURIFICATION: Antibody is purified by peptide affinity chromatography method.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
October 2, 2021 1 https://www.prosci-inc.com/e2f5-antibody-31-226.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.
CONCENTRATION: batch dependent
For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F5 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
OFFICIAL SYMBOL: E2F5
ALTERNATE NAMES: E2F5, E2F-5
ACCESSION NO.: NP_001942
PROTEIN GI NO.: 134142811
GENE ID: 1875
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines BACKGROUND: interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
REFERENCES: 1) Ohtani, N., et al., (2003) J. Cell Biol. 162 (2), 173-183.
ANTIBODIES FOR RESEARCH USE ONLY.
For additional information, visit ProSci's Terms & Conditions Page.
October 2, 2021 2 https://www.prosci-inc.com/e2f5-antibody-31-226.html