E2F5 Antibody Cat. No.: 31-226

E2F5 Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human E2F5.

TESTED APPLICATIONS: ELISA, WB

E2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can APPLICATIONS: be used for detection of E2F5 by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

PREDICTED MOLECULAR 38 kDa WEIGHT:

Properties

PURIFICATION: Antibody is purified by peptide affinity chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

October 2, 2021 1 https://www.prosci-inc.com/e2f5-antibody-31-226.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F5 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: E2F5

ALTERNATE NAMES: E2F5, -5

ACCESSION NO.: NP_001942

PROTEIN GI NO.: 134142811

GENE ID: 1875

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines BACKGROUND: interaction with the differentiation regulated proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to , a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

REFERENCES: 1) Ohtani, N., et al., (2003) J. Cell Biol. 162 (2), 173-183.

ANTIBODIES FOR RESEARCH USE ONLY.

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