E2F5 Antibody Cat. No.: 31-226 E2F5 Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human E2F5. TESTED APPLICATIONS: ELISA, WB E2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can APPLICATIONS: be used for detection of E2F5 by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 38 kDa WEIGHT: Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid October 2, 2021 1 https://www.prosci-inc.com/e2f5-antibody-31-226.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F5 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: E2F5 ALTERNATE NAMES: E2F5, E2F-5 ACCESSION NO.: NP_001942 PROTEIN GI NO.: 134142811 GENE ID: 1875 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines BACKGROUND: interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. REFERENCES: 1) Ohtani, N., et al., (2003) J. Cell Biol. 162 (2), 173-183. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 2, 2021 2 https://www.prosci-inc.com/e2f5-antibody-31-226.html.