Interactions of Zinc with the Intestinal Epithelium - Effects On
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Aberrant Expression of ZIP and Znt Zinc Transporters in Urotsa Cells Transformed to Malignant Cells by Cadmium
Article Aberrant Expression of ZIP and ZnT Zinc Transporters in UROtsa Cells Transformed to Malignant Cells by Cadmium Soisungwan Satarug 1,2,*, Scott H. Garrett 2, Seema Somji 2, Mary Ann Sens 2 and Donald A. Sens 2 1 Kidney Disease Research Collaborative, Centre for Health Service Research, The University of Queensland Translational Research Institute, Woolloongabba, Brisbane 4102, Australia 2 Department of Pathology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA; [email protected] (S.H.G.); [email protected] (S.S.); [email protected] (M.A.S.); [email protected] (D.A.S.) * Correspondence: [email protected] Abstract: Maintenance of zinc homeostasis is pivotal to the regulation of cell growth, differentiation, apoptosis, and defense mechanisms. In mammalian cells, control of cellular zinc homeostasis is through zinc uptake, zinc secretion, and zinc compartmentalization, mediated by metal transporters of the Zrt-/Irt-like protein (ZIP) family and the Cation Diffusion Facilitators (CDF) or ZnT family. We quantified transcript levels of ZIP and ZnT zinc transporters expressed by non-tumorigenic UROtsa cells and compared with those expressed by UROtsa clones that were experimentally transformed to cancer cells by prolonged exposure to cadmium (Cd). Although expression of the ZIP8 gene in parent UROtsa cells was lower than ZIP14 (0.1 vs. 83 transcripts per 1000 β-actin transcripts), an increased expression of ZIP8 concurrent with a reduction in expression of one or two zinc influx transporters, namely ZIP1, ZIP2, and ZIP3, were seen in six out of seven transformed UROtsa clones. -
Targeting an Nrf2/G6pdh Pathway to Reverse Multi-Drug
TARGETING AN NRF2/G6PDH PATHWAY TO REVERSE MULTI-DRUG RESISTANCE IN DIFFUSE LARGE B-CELL LYMPHOMA A Dissertation by SEYEDHOSSEIN MOUSAVIFARD Submitted to the Office of Graduate and Professional Studies of Texas A&M University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Chair of Committee, Steve Maxwell Committee Members, James C. Sacchettini Raquel Sitcheran David W. Threadgill Warren Zimmer Head of Program, Warren Zimmer May 2017 Major Subject: Medical Sciences Copyright 2017 Seyed Hossein Mousavi-Fard ABSTRACT A leading cause of mortality in diffuse large B-cell lymphoma (DLBCL) patients is the development of resistance to the CHOP regimen, the anthracycline-based chemotherapy consisting of cyclophosphamide, doxorubicin, vincristine, and prednisone. Our first objective of this work was to investigate the impact of Nuclear factor erythroid–related factor 2 (Nrf2)/ glucose-6-phosphate dehydrogenase (G6PDH) pathway on CHOP-resistance in DLBCL cell lines. We provide evidence here that a Nrf2/G6PDH pathway plays a role in mediating CHOP resistance in DLBCL. We found that CHOP-resistant DLBCL cells expressed both higher Nrf2 and G6PDH activities and lower reactive oxygen (predominantly superoxide) levels than CHOP- sensitive cells. We hypothesized that increased activity of the Nrf2/G6PDH pathway leads to higher GSH production, a more reduced state (lower ROS), and CHOP-resistance. In support of our hypothesis, direct inhibition of G6PDH or knockdown of Nrf2/G6PDH lowered both NADPH and GSH levels, increased ROS, and reduced tolerance or CHOP-resistant cells to CHOP. We also present evidence that repeated cycles of CHOP treatment select for a small population of Nrf2High/G6PDHHigh/ROSLow cells that are more tolerant of CHOP and might be responsible for the emergence of chemoresistant tumors. -
Relationship Between Neuropathic Pain and Zinc
Global Drugs and Therapeutics Mini Review ISSN: 2398-970X Relationship between neuropathic pain and zinc ion Tomoya Kitayama* Department of Pharmacy, School of Pharmacy and Pharmaceutical Science, Mukogawa Women’s University, Japan Neuropathic pain characterized by spontaneous pain, pain zinc ion activates matrix metalloproteinases that convert pro-BDNF sensation and tactile allodynia. The disease arising from peripheral or to mature-BDNF [7]. Zinc ion ionophore pyrithione inhibits KCC2 spinal nerve injury, diabetes, or infection with herpes virus is a result activity in vitro [8]. In other word, increase of zinc ion induces of the final product of an altered peripheral, spinal, and supraspinal decrease KCC2 function. On the other hand, high synaptic zinc ion process for which the usual analgesics are not effective and novel regulated by zinc transporter-3 elevates KCC2 activity via activation analgesics are desired. of metabotropic zinc ion sensing receptor [9]. These reports suggest The past study indicated that reduction of chloride gradient that zinc ion concentration have an important relationship with across the neuronal membrane, which in turn leads to reduction of KCC2 function. Moreover, it is considered that the alteration of zinc anion reversal potential, occurred in neurons of the superficial dorsal concentration modulates pain signaling. horn following a peripheral nerve injury [1]. The mechanism of the + - We previously detected by microarray method that partial sciatic change is down-regulation of K -Cl -cotransporter-2 (KCC2), which is nerve ligation surgery induces the decreased expression of slc30a1 (zinc potassium-chloride exporter, in spinal lamina I [1]. Similarly, the anion transporter 1, ZnT1) mRNA. The down regulation of ZnT1 gene was gradient is induced by brain-derived neurotrophic factor (BDNF) in relationship with BDNF-TrkB-KCC2 cascade reaction in astrocyte neuropathic pain model animals [2]. -
( 12 ) United States Patent
US007459539B2 ( 12) United States Patent ( 10 ) Patent No. : US 7 , 459 ,539 B2 Challita - Eid et al. (45 ) Date of Patent: Dec. 2 , 2008 ( 54 ) ANTIBODY THAT BINDS ZINC WO WO 03050236 6 / 2003 TRANSPORTER PROTEIN 108P5H8 ( 75 ) Inventors : Pia M . Challita - Eid , Encino , CA (US ) ; OTHER PUBLICATIONS Mary Faris , Los Angeles , CA (US ) ; Murgia et al . Cloning, expression , and vesicular localization of zinc Daniel E . H . Afar , Brisbane , CA (US ) ; transporter Dri 27 /ZnT4 in intestinal tissue and cells . Am J Physiol Rene S . Hubert , Los Angeles , CA (US ) ; 277 (Gastrointest Liver Physiol 40 ) : G1231 -61239 , 1999 . * Steve Chappell Mitchell, Gurnee , IL Kaufman et al. Blood 9 : 3178 -3184 , 1999 . * (US ) ; Elana Levin , Los Angeles, CA Wang et al . Rapid analysis of gene expression (RAGE ) facilitates (US ) ; Karen Jane Meyrick Morrison , universal expression profiling . Nucleic Acids Res 27 (23 ) : 4609 4618 , 1999 . * Santa Monica , CA (US ) ; Arthur B . Campbell et al. Totipotency of multipotentiality of cultured cells : Raitano , Los Angeles , CA (US ) ; Aya applications and progress . Theriogenology 47 : 63 -72 , 1997 . * Jakobovits , Beverly Hills , CA (US ) Moore , G . Genetically engineered antibodies. Clin Chem 35 ( 9 ) : 1849 - 1853 , 1989 . * (73 ) Assignee : Agensys, Inc ., Santa Monica , CA (US ) Dillman et al .Monoclonal antibodies in the treatment ofmalignancy : basic concepts and recent developments . Cancer Invest 19 ( 8 ) : 833 ( * ) Notice: Subject to any disclaimer, the term of this 841, 2001. * patent is extended or adjusted under 35 Skolnick et al. From genes to protein structure and function : novel U . S . C . 154 ( b ) by 1273 days . applications of computational approaches in the genomic era . -
The Effect of Acid on the Dynamics of Intracellular Zinc and the Marker Expressions Of
The Effect of Acid on the Dynamics of Intracellular Zinc and the Marker Expressions of Pluripotency in Somatic Cells A thesis presented to the faculty of the College of Arts and Sciences of Ohio University In partial fulfillment of the requirements for the degree Master of Science Yuli Hu April 2021 © 2021 Yuli Hu. All Rights Reserved. 2 This thesis titled The Effect of Acid on the Dynamics of Intracellular Zinc and the Marker Expressions of Pluripotency in Somatic Cells by YULI HU has been approved for the Department of Biological Sciences and the College of Arts and Sciences by Yang V. Li Professor of Biomedical Sciences Florenz Plassmann Dean, College of Arts and Sciences 3 Abstract YULI HU, M.S., April 2021, Biological Sciences The Effect of Acid on the Dynamics of Intracellular Zinc and the Marker Expressions of Pluripotency in Somatic Cells Director of Thesis: Yang V. Li Microenvironmental pH is one of the factors that affect the stability of zinc- protein binding. The tight binding between zinc and proteins is favored by the basic pH, whereas acidic pH favors a loose bound, and treatment of strong acid results in the dissociation of zinc. Physiologically, the stomach uses a very acidic pH to digest food which results in a high amount of soluble zinc in the stomach. Whether or not zinc co- present with acid and the effect of zinc on the gastric lining has rarely been discussed. In my experiments, acidic treatment induced the expression of a pluripotent marker in primary cultured gastric cells. It also stimulated the release of intracellular zinc, suggesting that acidic pH supported protein expression through dynamic zinc regulation. -
The Influence of Dietary Zinc Concentration During Periods Of
Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2019 The influence of dietary zinc concentration during periods of rapid growth induced by ractopamine hydrochloride or dietary energy and dietary fiber content on trace mineral metabolism and performance of beef steers Remy Nicole Carmichael Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Agriculture Commons, and the Animal Sciences Commons Recommended Citation Carmichael, Remy Nicole, "The influence of dietary zinc concentration during periods of rapid growth induced by ractopamine hydrochloride or dietary energy and dietary fiber content on trace mineral metabolism and performance of beef steers" (2019). Graduate Theses and Dissertations. 17416. https://lib.dr.iastate.edu/etd/17416 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. The influence of dietary zinc concentration during periods of rapid growth induced by ractopamine hydrochloride or dietary energy and dietary fiber content on trace mineral metabolism and performance of beef steers by Remy Nicole Carmichael A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Animal Science Program of Study Committee: Stephanie Hansen, Major Professor Nicholas Gabler Olivia Genther-Schroeder Elisabeth Huff-Lonergan Daniel Loy The student author, whose presentation of the scholarship herein was approved by the program of study committee, is solely responsible for the content of this dissertation. -
Clinical Study Molecular Characterization of Schizophrenia Viewed by Microarray Analysis of Gene Expression in Prefrontal Cortex
Neuron, Vol. 28, 53±67, October, 2000, Copyright 2000 by Cell Press Molecular Characterization of Clinical Study Schizophrenia Viewed by Microarray Analysis of Gene Expression in Prefrontal Cortex Ka roly Mirnics,*³§ Frank A. Middleton,* pus, superior temporal gyrus, and thalamus, appear to Adriana Marquez,* David A. Lewis,² be disturbed in this disorder (Harrison, 1999; McCarley and Pat Levitt*³ et al., 1999). In particular, a convergence of observations *Department of Neurobiology from clinical, neuroimaging, and postmortem studies ² Departments of Psychiatry and Neuroscience have implicated the dorsal prefrontal cortex (PFC) as a ³ PittArray major locus of dysfunction in schizophrenia (Weinberger University of Pittsburgh School of Medicine et al., 1986; Selemon et al., 1995; Andreasen et al., 1997; Pittsburgh, Pennsylvania 15261 Bertolino et al., 2000). Abnormal PFC function probably contributes to many of the cognitive disturbances in schizophrenia and appears to be related to altered syn- aptic structure and/or function in this cortical region. Summary For example, in subjects with schizophrenia, reductions in gray matter volume in the dorsal PFC have been ob- Microarray expression profiling of prefrontal cortex served in neuroimaging studies (Goldstein et al., 1999; from matched pairs of schizophrenic and control sub- Sanfilipo et al., 2000), and these volumetric changes jects and hierarchical data analysis revealed that tran- are associated with an increase in cell packing density scripts encoding proteins involved in the regulation (Selemon et al., 1995, 1998; Lewis and Lieberman, 2000) of presynaptic function (PSYN) were decreased in all but no change in total neuron number in the PFC (Pak- subjects with schizophrenia. Genes of the PSYN group kenberg, 1993). -
Frontiersin.Org 1 April 2015 | Volume 9 | Article 123 Saunders Et Al
ORIGINAL RESEARCH published: 28 April 2015 doi: 10.3389/fnins.2015.00123 Influx mechanisms in the embryonic and adult rat choroid plexus: a transcriptome study Norman R. Saunders 1*, Katarzyna M. Dziegielewska 1, Kjeld Møllgård 2, Mark D. Habgood 1, Matthew J. Wakefield 3, Helen Lindsay 4, Nathalie Stratzielle 5, Jean-Francois Ghersi-Egea 5 and Shane A. Liddelow 1, 6 1 Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, VIC, Australia, 2 Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark, 3 Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia, 4 Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland, 5 Lyon Neuroscience Research Center, INSERM U1028, Centre National de la Recherche Scientifique UMR5292, Université Lyon 1, Lyon, France, 6 Department of Neurobiology, Stanford University, Stanford, CA, USA The transcriptome of embryonic and adult rat lateral ventricular choroid plexus, using a combination of RNA-Sequencing and microarray data, was analyzed by functional groups of influx transporters, particularly solute carrier (SLC) transporters. RNA-Seq Edited by: Joana A. Palha, was performed at embryonic day (E) 15 and adult with additional data obtained at University of Minho, Portugal intermediate ages from microarray analysis. The largest represented functional group Reviewed by: in the embryo was amino acid transporters (twelve) with expression levels 2–98 times Fernanda Marques, University of Minho, Portugal greater than in the adult. In contrast, in the adult only six amino acid transporters Hanspeter Herzel, were up-regulated compared to the embryo and at more modest enrichment levels Humboldt University, Germany (<5-fold enrichment above E15). -
List & Label Preview File
L I N A R I S I n f o r m a t i o n B I O L O G I S C H E P R O D U K T E PRIMÄRANTIKÖRPER-Veterinär erkennen: Maus, verschiedene Labels alphabetisch geordnet Beschreibung Format Klon Wirt Isotyp Anwendung Menge ME Kat.Nr. Description Format Clone Host Isotype Application Quantity Cat.No. Mouse Anti-Cardiolipin Ig's -ve control for ELISA 1 ml ADI-5502 IgG Mouse 0,5 mg ADI-AMPT11-M-500 Anti-Cardiolipin Ig's +ve control for ELISA polyclonal Mouse 1 ml ADI-5503 CD27 PE (Armenian Hamster IgG1) Hamster 50 tests ADI-MCD027-PE Monoclonal Anti-VSV-G-Cy conjugate for Mouse 0,1 mg ADI-VSV11-Cy Immunofluorescence Interleukin-4 IgG Mouse 0,1 mg ADI-AB-10710 Anti-Myelin Oligodendrocyte Glycoprotein IgG Mouse 0,1 mg ADI-AB-19910 CD160 mAb, PE, , (mouse IgG2bk) Mouse 50 tests ADI-MCD160-PE CD81, Purified (mouse IgG1) Mouse 0,1 mg ADI-MCD081-UL Interleukin-2 receptor IgG Rat 0,1 mg ADI-AB-10310 Interleukin-2 IgG Rat 0,1 mg ADI-AB-10510 Interleukin-4 IgG Rat 0,1 mg ADI-AB-10810 Interleukin-10 IgG Rat 0,1 mg ADI-AB-11310 Interleukin-12p40 IgG Rat 0,1 mg ADI-AB-11410 CTLA-4 IgG Rat 0,1 mg ADI-AB-11610 CD80 IgG Rat 0,1 mg ADI-AB-13310 CD11a IgG Rat 0,1 mg ADI-AB-13510 CD11b-FITC IgG Rat 0,1 mg ADI-AB-13610 B220 IgG Rat 0,1 mg ADI-AB-13910 CD90 Thy-1.1 IgG Rat 0,1 mg ADI-AB-14010 CD90 Thy-1.2 IgG Rat 0,1 mg ADI-AB-14110 CD90 Thy-1 IgG Rat 0,1 mg ADI-AB-14210 CD4 IgG Rat 0,1 mg ADI-AB-16510 IFN-gamma IgG Rat 0,1 mg ADI-AB-16610 CD3 IgG Rat 0,1 mg ADI-AB-17510 Interleukin-12p75 IgG Rat 0,1 mg ADI-AB-20910 CD8b , PE-Cy5 (Clone CT-CD8b) (rat IgG2a) Rat 50 tests -
Maestra En Ciencias Biológicas
UNIVERSIDAD MICHOACANA DE SAN NICOLÁS DE HIDALGO FACULTAD DE BIOLOGÍA Programa institucional de Maestría en Ciencias Biológicas “Identificación de genes asociados a la síntesis de magnetita en la tortuga negra Chelonia agassizii.” TESIS QUE PARA OBTENER EL GRADO DE: Maestra en Ciencias Biológicas PRESENTA: Biol. María Guadalupe Rodríguez Jiménez Directora de Tesis: Dra. Alma Lilia Fuentes Farías Co-director: Dr. Jesús Campos García Morelia, Michoacán. Marzo del 2014 1 AGRADECIMIENTOS A Dios por darme la dicha de tener una hermosa familia, a mis padres y hermanos por todo el apoyo incondicional durante todos estos años y por haber compartido conmigo momentos inolvidables. A la Dra. Alma Lilia Fuentes Farías y al Dr. Gabriel Gutiérrez Ospina por darme el espacio y la oportunidad de integrarme a su equipo de trabajo, por todo el apoyo brindado y el esfuerzo dedicado al proyecto durante todo este tiempo. Al Dr. Jesús Campos García por recibirme en su Laboratorio de Biotecnología Microbiana en el IIQB, por las horas incansables de trabajo y esfuerzo dedicado al proyecto. Al Dr. Alejandro Bravo Patiño y a la Dra. Esperanza Meléndez Herrera, por sus valiosos comentarios y sugerencias que sin duda enriquecieron de manera sustancial al trabajo. A la M.C. Alma Laura Díaz Pérez, por el apoyo brindado, sus valiosas aportaciones y sugerencias durante la fase experimental. Al Dr. Jesús Ramírez Santos, por las sugerencias y el apoyo técnico brindado durante el proyecto. Al Dr. Irvin Jácome y al Dr. Víctor Meza por el apoyo brindado en la fase de expresión de genes en el trabajo. A todos mis amigos, compañeros de laboratorio por su apoyo y compañía. -
Roles of the Plant Cell Wall in Powdery Mildew Disease
Roles of the plant cell wall in powdery mildew disease resistance in Arabidopsis thaliana: PMR5 (POWDERY MILDEW RESISTANT 5) affects the acetylation of cell wall pectin By Candice Cherk Lim A dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Plant Biology in the Graduate Division of the University of California, Berkeley Committee in charge: Professor Shauna Somerville, Chair Professor Patricia Zambryski Associate Professor Mary Wildermuth Professor James Berger Spring 2013 Abstract Roles of the plant cell wall in powdery mildew disease resistance in Arabidopsis thaliana: PMR5 (POWDERY MILDEW RESISTANT 5) affects the acetylation of cell wall pectin by Candice Cherk Lim Doctor of Philosophy in Plant Biology University of California, Berkeley Professor Shauna Somerville, Chair The pmr5 (powdery mildew resistant 5) mutant was found in a screen for genes involved in susceptibility to Golovinomyces cichoracearum, a biotrophic pathogen that infects Arabidopsis. PMR5 is a member of the TBL (TRICHOME BIREFRINGENCE LIKE) family, which is composed of 46 functionally uncharacterized plant-specific proteins. Initial characterization of this mutant showed that pmr5-mediated disease resistance acts independently of the salicylic acid, jasmonic acid, and ethylene signal transduction pathways, and that there are changes in the pmr5 cell wall that may be linked to the gain of resistance in the mutant. Specifically, PMR5 may be affecting cell wall pectin by acetylation. Characterization of the pmr5 cell wall has revealed changes in pectin composition and a decrease in acetylation. This is corroborated by the ability of heterologously expressed PMR5 protein to bind to pectin, with decreased binding affinity to acetylated pectin. -
Differential Expression of Zinc Transporters Accompanies the Differentiation of C2C12 Myoblasts
University of Massachusetts Medical School eScholarship@UMMS Open Access Articles Open Access Publications by UMMS Authors 2018-9 Differential expression of zinc transporters accompanies the differentiation of C2C12 myoblasts Amanda L. Paskavitz University of Massachusetts Medical School Et al. Let us know how access to this document benefits ou.y Follow this and additional works at: https://escholarship.umassmed.edu/oapubs Part of the Cell Biology Commons, Cells Commons, Developmental Biology Commons, and the Molecular Biology Commons Repository Citation Paskavitz AL, Quintana J, Cangussu D, Tavera-Montanez C, Xiao Y, Ortiz-Mirnada S, Navea JG, Padilla- Benavides T. (2018). Differential expression of zinc transporters accompanies the differentiation of C2C12 myoblasts. Open Access Articles. https://doi.org/10.1016/j.jtemb.2018.04.024. Retrieved from https://escholarship.umassmed.edu/oapubs/3605 Creative Commons License This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License. This material is brought to you by eScholarship@UMMS. It has been accepted for inclusion in Open Access Articles by an authorized administrator of eScholarship@UMMS. For more information, please contact [email protected]. Journal of Trace Elements in Medicine and Biology 49 (2018) 27–34 Contents lists available at ScienceDirect Journal of Trace Elements in Medicine and Biology journal homepage: www.elsevier.com/locate/jtemb Molecular biology Differential expression of zinc transporters accompanies the differentiation