plants Article In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss] Chitralekha Shyam 1, Manoj Kumar Tripathi 2,*, Sushma Tiwari 2, Niraj Tripathi 3, Ravindra Singh Solanki 2, Swapnil Sapre 4 , Ashok Ahuja 2 and Sharad Tiwari 4 1 Department of Genetics & Plant Breeding, College of Agriculture, RVS Agriculture University, Gwalior 474002, India;
[email protected] 2 Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agriculture University, Gwalior 474002, India;
[email protected] (S.T.);
[email protected] (R.S.S.);
[email protected] (A.A.) 3 Directorate of Research Services, JN Agriculture University, Jabalpur 482004, India;
[email protected] 4 Biotechnology Centre, JN Agriculture University, Jabalpur 482004, India;
[email protected] (S.S.);
[email protected] (S.T.) * Correspondence:
[email protected] Abstract: Brassica juncea is a crucial cultivated mustard species and principal oilseed crop of India and Madhya Pradesh, grown for diverse vegetables, condiments, and oilseeds. Somaclonal variation was explored as a probable source of additional variability for the manipulation of fatty acids, especially low erucic acid contents that may be valuable for this commercially important plant species. The Citation: Shyam, C.; Tripathi, M.K.; plantlets regenerated from tissue cultures (R0), their R1 generation and respective parental lines Tiwari, S.; Tripathi, N.; Solanki, R.S.; were compared for morpho-physiological traits and fatty acid profile for the probable existence of Sapre, S.; Ahuja, A.; Tiwari, S. In Vitro somaclonal variations. The first putative somaclone derived from genotype CS54 contained 5.48% Production of Somaclones with and 5.52% erucic acid in R and R regenerants, respectively, compared to the mother plant (41.36%).