Contents Vol. 72, No. 10 October 1975

INFORMATION TO CONTRIBUTORS ...... iv...... I...... i-i AUTfHOR INDEX ...... 4190 Physical Sciences APPLIED Adiabatic evolution of plasma equilibrium (bifurcation/isolation/weak solution/generalized differential MATHEMATICS equation/Tokamak) ...... H. Grad, P.N. Hu, and D. C. Stevens 3789-3793 CHEMISTRY Theoretical studies of metal- interactions: Interaction of Li+, Na+, K+, Be++, Mg++, and Ca++ with H2PO4- and (CH30)2PO2-: Implications for solvation (metal binding/phos- phate complexes/molecular orbital theory) ...... Dennis S. Marynick and Henry F. Schaefer III 3794-3798 Maximum-valence radii of transition metals (covalent radii/enneacovalence) ...... Linus Pauling 3799-3801 Model of protein folding: Inclusion of short-, medium-, and long-range interactions (mechanism offolding/ contact free energies/range of interactions/Monte Carlo) ...... Seiji Tanaka and HaroldA. Scheraga 3802-3806 Matrix method for fhActuations and noise in kinetic systems (cross correlation function/noise power spectrum matrix/relaxation matrix/biochemical reaction kinetics/muscle contraction) .Yi-der Chen 3807-3811 MATHEMATICS Point estimates for probability moments (approximate methods/finite differences/numerical methods/prob- abilities) ...... - ;Emilio Rosenblueth 3812-3814 General almost automorphy (almost automorphic functions/almost periodic functions/minimal functions/ -strong solutions/weak solution).Salomon Bochner 3815-3818 STATISTICS Partial knowledge, entropy, and estimation (prior information/constrained entropy maximization) ...... J amesMacQueen and Jacob Marschak 3819-3824 Stochastic models for the Earth's relief, the shape and the fractal dimension of the coastlines, and the num- ber-area rule for islands (mathematical geomorphology/Hausdorff dimensions/Brownian random surface/ Gaussian processes) . Benoit B. Mandelbrot 38253828 Biological Sciences BIOCHEMISTRY Control of oscillating glycolysis of yeast by stochastic, periodic, and steady source of substrate: A model and experimental study (phosphofructokinase/entrainment/allosteric model/dissipative structures) ......

...... Arnold Boiteux, Albert-Goldbeter, and Benno Hess 3829-3833 Catalysis by : Evidence that-the rate-limiting step for acylation with certain substrates precedes general acid-base catalysis ( mechanism/diffusion control/induced-fit conformational change/pH dependence/deuterium oxide'isotope effects) ...... Terrone L. Rqsenberry 3834-3838 Translation of thyroglobulin 33S messenger RNA as a means of determining thyroglobulin quaternary struc- -ture (Xenopus oocyte/tryptic peptides/19 thyroglobulin)...... G. Vassart, S. Refetoff, H. Brocas, C. Dinsart, and J. E. Dumont 3839-3843 Mobility of cholera toxin receptors on rat lymphocyte membranes (immunofluorescence/cap formation/ade- nylate cyclase/gqngliosides/ganglioside-agarose) ...... S. W. Craig and P. Cuatrecasas 3844-3848 Partial purification of a glucocorticoid receptor (hormones/steroids/proteins/affinity chromatography) ...... Douglas Failla, Gordon M. Tomkins, and Daniel V. Santi 3849-3852 Imbalanced globin chain synthesis.,in heterozygous fl-thalassemic bone marrow (f3 thalassemia/proteolytic degradation/white cell proteins) ...... G. Chalevelakis, J. B. Clegg, and D. J. Weatherall 3853-3857 Light-dependent absorption and selective scattering changes at 518 nm in chloroplast thylakoid membranes (light scattering/518 nm absorbance change/conformational change) ...... S. W Thorne, G. Horvath, A. Kahn, and N. K. Boardman 3858-3862 DNA replication in vitro starting with an intact X174 phage (membranes/phage infection) ......

...... S. Michal Jazwinski and Arthur Kornberg 3863-3867 Restriction analysis of mitochondrial DNA from grande and genetically characterized cytoplas- mic petite clones of Saccharomyces cerevisiae (agarose gel electrophoresis/EcoRI and H. indIII endonu- cleases/antibiotic resistance markers/multiple deletions) ...... -Richard Morimoto, Alfred Lewin, Huey-Juang Hsu, Murray Rabinowitz, and Hiroshi Fukuhara 3868-3872 Very short-lived and stable mRNAs from resting human lymphocytes [RNA(+)poly(A)/mRNA half-life] ...... ,.Shelby L. Berger and Herbert L. Cooper 3873-3877 Human pituitary growth hormone: A biologically active hendekakaihekaton peptide fragment correspond- ing to amino-acid residues 15-125 in the hormone molecule (ornithine decarboxylase/tibia test/prolactin activity/complement fixation/circular dichroism) ...... Choh Hao Li 3878-3882 Oxygen and cyclic in human umbilical artery (cGMP/cAMP/calciutn) ...... Ronald.... L Clyman, Adam S. Blacksin, Vincent C. Manganiello, an~d Martha Vaughan 3883-3887 Neutron scattering measurements of separation and shape of proteins in 30S ribosomal subunit of Esche- richia coli: S2-S5, S5-S8, S3-S7 (ribosome structure/pair separation distances) ...... Donald M. Engelman, Peter B. Moore, and Benno P. Schoenborn '3888-3892 Relationship between in vivo degradative rates and isoelectric points of proteins (protein degradation/mam- malian cells/isoelectric focusing) .:...... J. Fred Dice and Alfred L. Goldberg 3893-3897 Cell-free synthesis of human interferon [protein synthesis/poly(A)-containing mRNA/human fibroblasts/ poly(I)-poly(C)/oligo(dT)-celluloseI ... Sidney Pestka, James McInnes, Edward A. Havell, and Jan Vilcek 3898-3901 (Continued on inside back cover) Contents (continued from outside back cover) Major nonhistone proteins of rat liver chromatin: Preliminiary identification of myosin, actin, tubulin, and tropomyosin (contractile proteins/endogenous protease of chromatin) ...... Angeline S. Douvas, Christina A. Harrington, and James Bonner 3902-3906 .X174 DNA-dependent DNA synthesis in vitro: Requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli (DNA replication/PIbac and Plbacban mutants/A:TP/rifampicin) ...... Heinz Schuster, Maria Mikolajczyk, Jean Rohrschneider, and Brigitte Geschke 3907-3911 Yeast manno-protein biosynthesis: Solubilization and selective assay of four mannosyltransferases (multi- mannosyltransferases/enzyme solubilization/mannan biosynthesis) ...... Tasuku Nakajima and Clinton E. Ballou 3912-3916 Position-specific oxygenation of benzo[a] pyrene by different forms of purified cytochrome P-450 from rabbit liver (chemical carcinogenesis/detoxification/hydroxylases/hepatic microsomes/aryl hydrocarbons) ...... F. J. Wiebel, J. K. Selkirk, H. F. Gelboin, D. A. Haugen, T. A. van der fHoeven, and M. J. Coon 3917-3920 Role of leucyl-tRNA synthetase in regulation of branched-chain amino-acid transport (Escherichia coli/ membrane binding proteins) ...... Steven C. Quay, Ellis L. Kline, and Dale L. Oxender 3921-3924 Direct identification of the calcium-binding amino acid,. y-carboxyglutamate, in mineralized tissue (pro- thrombin/vitamin K/bone/barium sulfate/alkaline hydrolysis) ...... Peter V. Hauschka, Jane B. Lian, and Paul M. Gallop 3925-3929 Intramolecular arsanilazotyrosine-248-Zn complex of carboxypeptidase A: A monitor of catalytic events (in- tramolecular coordination complex/temperature-jump stopped-flow kinetics/spectral probe/catalytic me- chanism) ...... Lawrence W. Harrison, David S. Auld, and Bert L. Vallee 3930-3933 Translation of hormone-induced messenger RNA in amphibian oocytes: I: Induction by estrogen of mes- senger RNA encoded for vitellogenic protein in the liver of the male African clawed toad (Xenopus laevis) [estrogen action/polyribosomes/poly(A)-rich RNA/microinjection/lipovitellin/phosvitin] ...... Kenneth D. Lanclos and Terrell H. Hamilton 3934-3938 Methylation of a membrane protein involved in bacterial chemotaxis (Escherichia coli/methionine/flagella/ electrophoresis) ...... Edward N. Kort, Michael F. Goy, Steven H. Larsen, and Julius Adler 3939-3943 De novo synthesis of a polymer of deoxyadenylate and deoxythymidylate by calf thymus DNA polymerase a [poly(dA-dT)/DNA unwinding protein] ...... David Henner and John J. Furth 3944-3946 Polypeptide chain initiation in eukaryotes:. Initiation factor MP in Artemia salina embryos (cysts/brine shrimp eggs/eukaryotic initiation factors/protein synthesis/development) ...... Witold Filipowicz, JoseM. Sierra, and Severo Ochoa 3947-3951 Chitin synthetase zymogen is attached to the yeast plasma membrane (yeast septum/gradient centrifuga- tion/concanavalin A/glutaraldehyde) ...... Angel Duran, Blair Bowers, and Enrico Cabib 3952-3955. Regulation of synthesis of hepatic fatty acid synthetase: Binding of fatty acid synthetase antibodies to poly- somes (fatty acid synthetase structure/enzyme induction/animal nutrition) ...... ;A.W. Alberts, A. W. Strauss,...... S. Hennessy, and P. R. Vagelos 3956-3960 Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene (Drosophila melanogaster DNA/recombinant DNA molecules/p.lasmids/18-28S rRNA genes/autoradiography) ...... Michael Grunstein and David S. Hogness 3961-3965 Dynamic and static quenching of 1,N6-ethenoadenine fluorescence in nicotinamide 1,N6-ethenoadenine di- and in 1,N6-etheno-9-[3-(indol-3-yl)propyl]adenine (1,N6-ethenoadenine derivatives/fluores- cence efficiency, emission, and lifetime/fluorescent coenzyme/ENAD+ /stacked conformations) ...... Bruce A. Gruber and Nelson J. Leonard 3966-3969 Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Esch- erichia coli (energy-coupling site/specificity of energy-coupling/orientation of vesicle membrane/mem- brane potentials) ...... Paul Stroobant and H. Ronald Kaback 3970-3974 Biosynthesis of mouse interferon by translation of its messenger RNA in a cell-free system [wheat germ ex- tract/in vitro products/antiviral activity/poly(I).poly(C)/spermine effect] ...... M. N. Thang, D. C. Thang, E. De Maeyer, and L. Montagnier -3975-3977 Analysis of DNA of isolated chromatin subunits (/DNA reassociation/DNA electrophoresis) ...... Elizabeth Lacy and Richard Axel 3978-3982

BIOPHYSICS The turning wheel: A study in steady states (macromolecule/functional linkage) ...... Jeffries Wyman 3983-3987 Components of 02-uptake by excised frog nerve dependent upon externally supplied sodium ions (compo- nents ofpassive influx/tetrodotoxin/activated efflux/ouabain) ...... Frank Brink, Jr. 3988-3992 A theory of the chain melting phase transition of aqueous phospholipid dispersions (lipids/statistical thermo- dynamics) ...... Russell E. Jacobs, Bruce Hudson, and Hans C. Andersen 3993-3997 Magnitude of subunit inequivalence for oxygenA releqse from hemoglobin:Reinvestigation ofthe oxygen-pulse experiment (hemoglobin Kansas/stopped-flow kinetics/inositol hexaphosphate) ...... J. M. Salhany, C. L. Castillo, M. J. McDonald, and Q. H. Gibson 3998-4002 Determination of the iron-sulfur distances in rubredoxin by x-ray absorption spectroscopy (protein struc- ture/iron-sulfurproteins) ...... R. G. Shulman, P. Eisenberger, W. E. Blumberg, and N. A. Stombaugh 4003-4007 CELL BIOLOGY Infection of preimplantation mouse embryos and of newborn mice with leukemia virus:Tissue distribution of viral DNA and RNA and leukemogenesis in the adult animal (in vitro infection of 4-8 cell embryos with Moloney murine leukemia virus/development/leukemia/DNA and RNA annealing kinetics) ...... RudolfJaenisch, Hung Fan, and Byron Croker 4008-4012 Evidence for the translocation of 5'-nucleotidase across..hepatic membranes in vivo (Golgi fractions/mem- brane protein orientation/cytochemistry/antibody/concanavalin A) ...... James S. Little and Christopher C. Widnell 4013-4017 (Continued on page 4192) Contents (continued from inside back cover) New approach to antifolate treatment of certain cancers as demonstrated in tissue culture (methotrexate/N5- methyltetrahydrofolate/cancer chemotherapy) ...... Richard M. Halpern, Barbara C. Halpern, Brian R. Clark, ...... Hilary Ashe, Dorothy N. Hardy, Pamela Y. Jenkinson, Shao-Chia Chou, and Roberts A. Smith 4018-4022 Assembly of microtubules onto kinetochores of isolated mitotic chromosomes of HeLa cells (electron micro- scopy/tubulin/phosphotungstic acid staining) ...... Bruce R. Telzer, Montrose J. Moses, and Joel L. Rosenbaum 4023-4027 Synthesis of acetylcholine receptor during differentiation of cultured embryonic muscle cells (a-neurotoxins/ affinity chromatography/immunological crossreaction) ...... J. P. Merlie, A. Sobel, J. P. Changeux, and F. Gros 4028-4032 Specific endocrine and hormonal receptor changes associated with liver regeneration in adult rats (insulin/ glucagon/thyroxin/membrane receptors) ...... H. Leffert, N. M. Alexander, G. Faloona, B. Rubalcava, and R. Unger 4033-4036 Conditions controlling commitment of differentiation in Bacillus megaterium (development/sporulation/ nutrient effects) ...... Elisabeth B. Freese, Peter Cooney, and Ernst Freese 4037-4041 Increased cellular levels of spermidine or spermine are required for optimal DNA synthesis in lymphocytes activated by concanavalin A [methylglyoxal bis(guanylhydrazone)/cell proliferation] ...... Robert H. Fillingame, Caroline M. Jorstad, and David R. Morris 4042-4045 Membrane particles on fracture faces of frozen myelin (freeze-fracture/proteolipid/myelin basic protein/de- myelination/membrane proteins) ...... Pedro Pinto da Silva and Rodman G. Miller 4046-4050 Effect of oncogenic virus on muscle differentiation (myogenesis/temperature-sensitive Rous sarcoma virus/ transformed myogenic cells/creatine kinase) ... H. Holtzer, J. Biehl, G. Yeoh, R. Meganathan, and A. Kaji 4051-4055 Identification of aldosterone-induced proteins in the toad's urinary bladder (sodium transport/corticoste- rone/protein synthesis) ...... Walter N. Scott and Victor S. Sapirstein 4056-4060 GENETICS Normal and mutant thermotaxis in the nematode Caenorhabditis elegans (behavior/mutants/temperature/ chemotaxis) ...... Edward M. Hedgecock and Richard L. Russell 4061-4065 Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia (5- bromodeoxyuridine/33258 Hoechst fluorescence/mitomycin C/ethylmethane sulfonate/DNA repair) ...... Samuel A. Latt, Gail Stetten, Lois A. Juergens, George R. Buchanan, and Park S. Gerald 4066-4070 Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the con- comitant treatment with bacteriophage T4 endonuclease V and HVJ(Sendai virus) (excision repair/com- plementation group/incision) ...... Kiyoji Tanaka, Mutsuo Sekiguchi, and Yoshio Okada 4071-4075 Meiosis in Phycomyces (linkage/map units/centromere) ...... Arturo P. Eslava, Maria Isabel Alvarez, and Max Delbruck 4076-4080 Possible peptide chain termination mutants in thymidine kinase gene of a mammalian virus, herpes simplex virus (bromodeoxyuridine/amber mutants/suppression) ...... Wilma P. Summers, Michael Wagner, and William C. Summers 4081-4084 Inheritability of plasmids and population dynamics of cultured cells (population genetics/branching pro- cesses/bacteria/mutations) ...... Thomas F. Anderson and Edward Lustbader 4085-4089 Evolution of type C viral genes: Preservation of ancestral murine type C viral sequences in pig cellular DNA (porcine type C virus/trans-species gene transfer/"fossil" genes/DNA-DNA hybridization) ...... RaoulE. Benveniste and George J. Todaro 4090-4094 Linkage disequilibrium over space and time in natural populations of Drosophila montana (/electro- phoretic variants/neutrality versus selection hypothesis) ...... William K. Baker 4095-4099 IMMUNOLOGY Protection of lethally irradiated mice with allogeneic fetal liver cells: Influence of irradiation dose on im- munologic reconstitution (fetal liver transplantation/cellular engineering/radiation-induced immunode- ficiency/hematologic reconstitution/stem cell reconstitution) ...... Ozden Tulunay, Robert A. Good, and Edmond J. Yunis 4100-4104 Comparison between macrophage activation and enhancement of nonspecific resistance to tumors by myco- bacterial immunoadjuvants (nonspecific tumor immunity) ...... D. Juy and L. Chedid 4105-4109 Cerebellar cell-surface antigens of mouse brain (reaggregated cell culture/cerebellar mutants/differentia- tion/cytotoxicity/immunofluorescence) ...... Nicholas W. Seeds 4110-4114 Immunochemical evidence for a common variable region in three immunoglobulin classes in the same indi- vidual (idiotype/M-component) ...... Anders 0. Grubb and Olle H. Zettervall 4115-4118 Antibody to terminal deoxynucleotidyl (crosslinked antigen/antibody crossreaction/thymus/ bone marrow/leukemic lymphoblasts) ...... F. J. Bollum 4119-4122 Purification and synthesis of eosinophilotactic tetrapeptides of human lung tissue: Identification as eosin- ophil chemotactic factor of anaphylaxis (leukocyte chemotaxis/leukocyte deactivation/anaphylactic med- iators/acidic peptides of lung) ...... Edward J. Goetzl and K. Frank Austen 4123-4127 A unique complement derived chemotactic factor for tumor cells (cancer cells/chemotaxis) ...... Alberto G. Romualdez, Jr. and Peter A. Ward 4128-4132 MEDICAL Purification of RNA-instructed DNA polymerase from human leukemic spleens (reverse transcriptase/leu- SCIENCES kemia) ... S. S. Witkin, T. Ohno, and S. Spiegelman 4133-4136 Growth and differentiation in culture of leukemic leukocytes from a patient with acute myelogenous leukem- ia and re-identification of type-C virus (liquid suspension culture/leukocyte growth-stimulating factor/ granulocyte differentiation/chromosome abnormality/RNA tumor virus) ...... Robert E. Gallagher, S. Zaki Salahuddin, William T. Hall, Kenneth B. McCredie, and Robert C. Gallo 4137-4141

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Hypoxanthine phosphoribosyltransferase deficiency: Association of reduced catalytic activity with reduced levels of immunologically detectable enzyme protein (Lesch-Nyhan syndrome/immunoprecipitation/ crossreactive material) ...... Katherine S. Upchurch, Albert Leyva, William J. Arnold, Edward W. Holmes, and William N. Kelley 4142-4146 Radioimmune determination of hypoxanthine phosphoribosyltransferase crossreacting material in erythro- cytes of Lesch-Nyhan patients (genetic disease/purine salvage enzyme/125I iodination) ...... GurdevS. Ghangasand Gregory Milman 4147-4150 Further comments on the ascorbic acid requirement (vitamin C/scurvy/dietary allowances/evolution) ...... Thomas H. Jukes 4151-4152 Bis-(N-maleimidomethyl) ether: An antisickling reagent (sickle cell anemia/hemoglobin) ...... Solomon J. Zak, ...... Gary R. Geller, Barbara Finkel, David P. Tukey, Michael K. McCormack, and William Krivit 4153-4156 MICROBIOLOGY Complete translation of poliovirus RNA in a eukaryotic cell-free system (protein synthesis/picornaviruses/ polyproteins/proteolysis) ...... Lydia Villa-Komaroff, Naomi Guttman, David Baltimore, and Harvey F. Lodish 4157-4161 Mechanism of action of penicillin: Triggering of the pneumococcal autolytic enzyme by inhibitors of cell wall synthesis (release of lipoteichoic acid/autolysin) ...... Alexander Tomasz and Susan Waks 4162-4166 PATHOLOGY Antibody nature of circulating inhibitor of plasma von Willebrand factor (factor VIII inhibitor/platelet-ag- gregating factor/transfusion response). . Robert D. Stratton, Robert H. Wagner, William P. Webster, and K. M. Brinkhous 4167-4171 PHYSIOLOGY Body weight and food intake at early estrus of rats on a high-fat diet (puberty/vaginal opening/caloric in- take/weanling/critical fatness) ...... R. E. Frisch, D. M. Hegsted, and K. Yoshinaga 4172-4176 Effect of hypothalamic deafferentation of thyrotropin-releasing hormone levels in rat brain (releasing hor- mone/radioimmunoassay/central nervous system/thyroid gland) ...... M. J. Brownstein, R. D. Utiger, M. Palkovits, andJ. S. Kizer 4177-4179 PSYCHOLOGY Neurohypophyseal hormones, analogs, and fragments: Their effect on puromycin-induced amnesia (mem- ory consolidation/peptides/structure-activity relationship/mice) ...... Roderich Walter, P. L. Hoffman, Josefa B. Flexner, and Louis B. Flexner 4180-4184 ZOOLOGY Chemoreceptors and feeding in calanoid copepods (Arthropoda: Crustacea) (sensillum/zooplankton/selec-

tive feeding/oil-spill dispersal) ...... Marc M. Friedman and J. Rudi Stricklei 4185-4188

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