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12 to 15 September - 2018 • Rio De Janeiro - Brazil ISSN 1517-8692 Impresso Especial 9912273897 - DR/SPM Sociedade Brasileira de Medicina do Esporte CORREIOS Revista Brasileira de Medicina do Esporte Brazilian Journal of Sports Medicine ANNALS OF THE 35TH FIMS WORLD CONGRESS OF SPORTS MEDiciNE 30TH BRAZILiaN CONGRESS OF SPORTS AND EXERciSE MEDiciNE 12 TO 15 SEPTEMBER - 2018 • RIO DE JANEIRO - BRAZIL FECHAMENTO AUTORIZADO PODE SER ABERTO PELA ECT SUPLEMENTO - VOLUME 24 – NO 5 – SETEMBRO/OUTUBRO DE 2018 Indexada na Scielo, Web of Science (ISI), SCOPUS, Index Copernicus, Excerpta Medica-EMBASE, Physical Education Index, SIRC-Sportdiscus eW LILACS Editor Chefe Editor Científico Medicina do Esporte aplicada na Traumato-Ortopedia Medicina do Esporte aplicada na Traumato-Ortopedia André Pedrinelli Ricardo Munir Nahas Professor Livre Docente do Departamento de Ortopedia e Traumatologia da Faculdade de Departamento de Ortopedia do Hospital Ipiranga (UGA II). Secretária de Saúde do Governo do Medicina da Universidade de São Paulo (FMUSP), São Paulo, SP, Brasil. Estado de São Paulo, São Paulo, SP, Brasil. Hospital 9 de julho, São Paulo, SP, Brasil. Editores Associados Medicina do Esporte aplicada na Reabilitação Medicina do Esporte aplicada na Clínica Julia Maria D’andrea Grave Daniel Jogaib Daher Professora Livre Docente do Instituto de Ortopedia e Traumatologia, Hospital das Clínicas da Instituto Dante Pazanezzi de Cardiologia, São Paulo, SP, Brasil. Faculdade de Medicina da Universidade de São Paulo (IOT HC-FMUSP), São Paulo, SP, Brasil. Laboratório de Estudo do Movimento (LEM), São Paulo, SP, Brasil. Medicina do Esporte aplicada na Fisiologia do Exercício Medicina do Esporte aplicada na Traumato-Ortopedia Paulo Sergio Martino Zogaib Tiago Lazzaretti Fernandes Médico da Disciplina de Medicina do Esporte e Atividade Física, Departamento de Ortopedia Professor Colaborador do Grupo de Medicina do Esporte, Instituto de Ortopedia e Traumatologia, e Traumatologia (DOT-EPM), Escola Paulista de Medicina, Universidade Federal de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (IOT HC-FMUSP), São Paulo, SP, Brasil. São Paulo, SP, Brasil. Conselho Editorial Membros Nacionais Arnaldo José Hernandez – Departamento de Ortopedia e Traumatologia da Faculdade de Medicina da Universidade de São Paulo. São Paulo, SP. Páblios Staduto Braga da Silva – Medicina do Exercício e do Esporte do Grupo Fleury. São Paulo, SP; Centro de Referência em Medicina do Exercício e do Esporte do Hospital 9 de Julho. São Paulo, SP. Roberto Yukio Ikemoto – Departamento de Ortopedia e Traumatologia do Hospital Ipiranga. São Paulo, SP; Grupo de Ombro e Cotovelo da Faculdade de Medicina do ABC. São Paulo, SP; Grupo de Ombro do Hospital Estadual Mário Covas de Santo André. São Paulo, SP. Antônio Claudio Lucas da Nóbrega – Universidade Federal Fluminense. Niterói, RJ. Claudio Aparício Silva Baptista - Secretaria Municipal de Esportes de São Paulo. São Paulo, SP. Cláudio Gil Soares de Araújo – Universidade Gama Filho. Rio de Janeiro, RJ. Edimar Favaro - Sociedade Paulista de Medicina do Exercício e do Esporte. São Paulo, SP. Eduardo Henrique De Rose – Comitê Olímpico Brasileiro. Porto Alegre, RS. Fernanda de Souza N. Sardinha Mendes – Universidade Federal Fluminense. Niterói, RJ. Flavia Meyer – Universidade Federal do Rio Grande do Sul. Porto Alegre, RS. João Ricardo Turra Magni – Grêmio Foot-ball Porto Alegrense. Porto Alegre, RS. José Blanco Herrera – Universidade Católica de Brasília. Brasília, DF. Leonardo Silva Roever Borges - Universidade Federal de Uberlândia. Uberlândia, MG. Luciano Rezende – Secretaria Municipal de Saúde. Vitória, ES. Marco Túlio de Mello – Universidade Federal de São Paulo. São Paulo, SP. Maria Augusta Peduti Dal Molin Kiss – Universidade de São Paulo. São Paulo, SP. Nabil Ghorayeb – Instituto Dante Pazzanese de Cardiologia. São Paulo, SP. Neiva Leite – Universidade Federal do Paraná. Curitiba, PR. Paulo de Tarso Veras Farinatti – Universidade do Estado do Rio de Janeiro. Rio de Janeiro, RJ. Renata Rodrigues Teixeira de Castro – Universidade Federal Fluminense. Niterói, RJ. Ricardo Sten - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS. Tales de Carvalho – Universidade Estadual de Santa Catarina. Florianópolis, SC. Vitor Keihan Rodrigues Matsudo – Centro de Estudos do Laboratório de Aptidão Física de São Caetano do Sul. São Caetano do Sul, SP. Membros Internacionais Dusan Hamar – Research Institute of Sports Science. Eslováquia. Frank I. Katch – University of Massachusetts. EUA. Italo Monetti – Organização Desportiva Sul-Americana. Uruguai. Jon W. Wiliamson – University of Texas Southwestern Medical Center at Dallas. EUA. José Veloso – Comitê Olímpico Uruguaio. Uruguai. Josef Niebauer – University of Leipzig. Alemanha. L. Britt Wilson – University of South Alabama. EUA. Michael J. Joyner – University Of Arizona. EUA. Timothy Noakes – University of Cape Town. África do Sul. Walter Frontera – Harvard Medical School. EUA. William D. Ross – Simon Fraser University. Canadá. Editor Consultivo Editora Executiva Arthur Tadeu de Assis – Atha Comunicação e Editora. São Paulo, SP, Brasil. Ana Carolina de Assis – Atha Comunicação e Editora. São Paulo, SP, Brasil. Realização: Apoio: Filiada à Associação Brasileira de Editores Científicos e à World Association of Medical Editors Ministério do Esporte Diretoria Presidente: Marcelo Bichels Leitão (PR); Vice-Presidente: Ivan Pacheco (RS); Secretário: Marco Aurelio Moraes de Souza Gomes (RJ); Diretor Científico: Jose Kawazoe Lazzoli (RJ); Diretora Financeira: Alana Cláudia Murilo Santos (PR); Diretor de Relações Comerciais: Roberto Teixeira Nahon Marinho (RJ); Diretor de Comunicação: Fernando Carmelo Torres (SP); Diretor de Regionais: Ricardo Galotti (SP); Diretor de Defesa Profissional: Jinmy Henry Ricaldi Rocha (SE) e Héldio Fortunato Gaspar de Freitas (SP); Presidente Eleito: Marcos Henrique Ferreira Laraya (SP). Ex-Presidentes Daniel Arkader Kopiler, Samir Salim Daher, Waldemar Areno, Eduardo Henrique de Rose, José Rizzo Pinto, Ruben Pimenta da Silva, José Luis Fraccaroli, Maeterlinck Rego Mendes, Maurício Bravo, João Gilberto Carazzato, João Ricardo Turra Magni, Marcelo Salazar, Marcos Aurélio Brazão de Oliveira (in memorian), Tales Carvalho, Ricardo Munir Nahas, Felix Albuquerque Drummond, Arnaldo José Hernandez, José Kawazoe Lazzoli, Jomar Brito Souza. Coordenação editorial, criação, diagramação e produção gráfica: Atha Comunicação e Editora - Tel/Fax: (11) 5087-9502 / 5579-5308 - E-mail: [email protected] ANNALS OF THE 35º CONGRESSO MUNDIAL DE MEDICINA DO ESPORTE FIMS - 30º CONGRESSO BRASILEIRO DE MEDICINA DO EXERCÍCIO E DO ESPORTE 01 ACUTE LONG DISTANCE TRAIL RUNNING INCREASES SERUM IL-6, IL-15 AND HSP72 LEVELS Introduction and Purpose: Myokines are important molecules secreted from skeletal muscle. Especially IL-6 is known to have a regulatory role in glucose metabolism, whereas IL-15 demonstrates its metabolic effects on fat metabolism. On the other hand, heat shock proteins play a role to chaperone misfolded and denatured proteins, in order to protect the protein homeostasis of the cell. In addition, increased Hsp72 levels has been shown to prevent insulin resistance. Although it is very clear that IL-6 increase following acute exercise, there are some contradictory results about the increase in IL-15 and Hsp72 levels. Long-distance trail runs are gaining more popularity each year as a large number of elite and recreational athletes from all age groups are involved in such events. Involving more uphill and downhill running is one of the characteristics of a trail run, therefore compared to city marathons, trail runs are more demanding and challenging. This research covers serum levels of three molecules that all together have effects on glucose and fat metabolism and cellular stress response following an acute long distance trail run. Material and Methods: The runners who attended Cappadocia Ultra-Trail® had to complete the 36-km course in less than 6 hours, during which the total climb was 940 m. The temperature on the race day was between 13-18 °C, with heavy rain. The air humidity on the race day was 68-93% and the wind speed was 13km/h on average. Blood samples were collected before and 15 minutes after the race. Total blood count was analyzed immediately, and rest of the samples were kept at room temperature for half an hour, then centrifuged at 1500 rpm for 10 minutes to obtain serum. Serum samples were stored in ice, transferred to the laboratory on the same day and stored at -80 ° C until the day of analysis. Serum IL-6, IL-15 and Hsp72 levels were measured by sandwich ELISA method with commercially available kits according to the manu- facturer’s instructions. All serum samples were tested in triplicate. Because of the low serum volume, IL-15 and Hsp72 were assayed only in 26 and 17 subjects, respectively. Corrections were made for each athlete to eliminate the effect of hemoconcentration according to the hematocrit values before and after the race. Results: A total of 37 athletes participated in the study (n = 37). Eleven of the athletes were female (29,72%) and 26 were male (70,27%). The mean age of the athletes was 38.9 ± 10.1; mean body mass indexes were 23.22 ± 2.21. After the race, the IL-6, IL-15 and Hsp72 serum concentrations showed a significant increase; 13.2 fold, 2.22 fold and 1,6 fold respectively (p<0.001, p<0.001, p<0.039 respectively). Discussion: It is well known that exercise promotes glucose uptake independent of insulin, increases insulin sensitivity, accelerates fat metabolism,
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