Detection of causative agents out using the ATB Expression ( «BioMerieux») Conclusion. The primary role of the β-HSA DNA contamination of hearth of erysipelas is and test systems ID32E, ID32Staph, ID32Strep, is indisputable, in the cases with complications depends on the presence of live staphylococci in focus in erysipelas patients ID32GN, identification of β-HS in groups A, B, C, mixt-infection can take a part in the etiology of on skin surface by bacteriological method and D, F and G (at Lancefield) - a latex agglutination the disease. PCR-RT with fluorescent Staphylococci on skin: PCR reaction(Pastorex Strep Kits; «Bio-Rad»,France) hybridization detection showed the highly informative content in the study of the blood and S.aureus No staphyl. S.epid./saprop. Volchkova E.V.1, Belaia O.F.1, Troickii V.I.1, The overall frequency of detection of different Svistunova Т.S.2,Potekaeva S.N.2, microorganisms (85%) in PCR in patients bulls punctuate. and it was more informative in Staphylococcus DNA in hearth: Domonova E.A.3 with erysipelas (n=60) identifying the microflora in patients with 1 erysipelas than bacteriological method. The 80% 32% # 7,0% # Sechenov First Moscow State Medical Staphylococci S.aureus-39% University, 2 Infectious Clinical Hospital №2, bacteriological method showed low efficacy in 74.5% MSSA:MRSA = 9:2 PCR results: 3Central Research Institute of Epidemiology, detecting of Str.pyogenes, however, positive S.epidermidis and/or Moscow, Russian Federation results of bacteriological research can serve as MRSA -2 - - S.saprophyticus-43% MRCoNS - 4 - MRCoNS -5 Erysipelas is a common infection of the skin an objective criterion of therapy correction based MRCoNS-20% MSSA - 7 MSSA -1 MSSA - 2 surface layer, unlike cellulitis and necrotizing on identified microbes. fasciitis, which also affect the subcutaneous Streptococci S.pyogenes - 33,3% Clinical and laboratory parameters in detection «Load” of Staphylococcus DNA per patient: tissue. The main causative agent is widely 47,06% Others – 4% of Staphylococcus (%) at erysipelas 1,1 0,4 # 0,07 # recognized as β-hemolytic streptococcus group Symptom Subgr.1 Subgr.2 Subgr.3 Others K.pneumoniae - 3 A (β-HSA) and G, but recently on discussed the Bacter. PCR+ no staphy- «Load” of all microbe DNA per patient: microbes P.aeruginosa - 3 participation of Staphylococcus aureus and method+ Lococci 22% A.baumanii - 2 1,4 0,8 0,3 # gram-negative bacteria in the development of Fever: 39,00,1 38,70,1 38, 90,2 0 erysipelas. Pathogen cultures isolation by C.normanensis - 1 max( С) # # - differences to subgr.1 (р≤0,05) bacteriological method in patients with C.musifaciens - 1 duration 9,20,6 8,10,9 9,61,5 (day) References: erysipelas is a difficult task, so in most cases of Proteus - 1 Erythema: 1,50,1 1,90,2 1,80,3 1.Ray G. T., Suaya J. A. and Baxter R. Inciden- erysipelas diagnosis is established by clinical Results. The bacteriological study demonst- onset(day) # ce, microbiology, and patient characteristics of and epidemiologic data. rated a wide range of the pathogens, preferen- duration 7,9 0,4 8,30,8 7,70,6 skin and soft-tissue infections in a U.S. popula- Objective: to study the etiological structure tially staphylococcus (S.epidermidis 22,2%, (day) tion: a retrospective population-based study. of the disease in patients with erysipelas of the S.aureus 22,2%, S.saprophyticus 11,1%, S.pyo- # - differences to subgr. 2 (p ≤ 0,05) BMC Infectious Diseases. 2013; 13:252-26. lower extremities by using bacteriological 2.Gunderson C.G., Martinello R.A. A systematic genes 2 patients, Str.dysgalacticeae equisimilis . method and PCR. Residual effects in the detection of staphylococci review of bacteremias in cellulitis & erysipelas. 1), mainly- in skin smears, while the informa- by bacteriological method and PCR Materials/methods. A total of 60 in-patients Journal of Infection. 2012; 64:148-155 tiveness of blood culture and bulls punctate was Subgr.1 Subgr.2 Subgr.3 with erysipelas of the lower limbs, mainly with Symptom 3.Larru B., Gerber J.S. Cutaneous bacterial low. Monoinfection - 48%, mixt-infection-10%. In Bacter.+ PCR + No staph. bullous hemorrhagic form, were examined. The Infections caused by Staphylococcus aureus PCR it was confirmed a leading role of β-HSA in Congestive 25,0% 28,6% and Streptococcus pyogenes in Infants and average age of patients was 53,6±1,7 years. 66,7% the development of bullous-hemorrhagic erysi- hyperemia # # children. Pediatr Clin N Am 61. 2014:457–478 We examined blood, smears from the skin in the pelas (61% in bull puncture), and in smears from 4.Troitskiy V.I., Erovichenkov A.A., Potekaeva area of the hearth and bulls punctate by using Prolonged the hearth surface -S.aureus (MSSA-6%, reparation, S.A., Svistunova T.S., Belaya O.F., Volchkova bacteriological method and PCR with fluorescent 25,9% 43,8% 7,1% MRSA-2%), MRCoNS (6%), but some other trophic E.V. Diversity of detected pathogens from pati- hybridization detection in real time. Identification microbes - only in combination with ulcers ents with erysipelas. Epidemiology and Infec- of isolated pure cultures to species was carried tious Diseases. 2015; 20 (2):34-7 [In Russian] Streptococcus and/or Staphylococcus. #- differences to subgr.1 (p ≤ 0,05)
VasiliyTroitsky: [email protected]