Anti-Symmetric Di-Methyl- H3 (Arg2) Mouse mAb

Catalog # PTM-693

General Information Images

Host species Mouse Dot Blot

Clonality Monoclonal Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Clone number / Primary ab incubation condition: 2 hours at room temperature Synonym (sy) Secondary ab: Goat Anti-Mouse IgG H&L Immunogen UniProt MW (kDa) Applications (HRP) species Immunogen peptide quantity: 1 ng, 4 ng, 16 ng Human P68431 17 WB Exposure time: 60 s The list of peptides is included in the table below: Product Usage Information No. Peptide No. Peptide Lane 1 H3R2me2 (sy) Lane 2 H3R2me1

Application Dilution Recommended species Lane 3 H3R2me2 (asy) Lane 4 H3K4ac Human, Mouse, Rat, Lane 5 H3K4cr Lane 6 H3K4hib WB 1:500-1:2000 Lane 7 H3K4me1 Lane 8 H3K4me2 Chlorocebus aethiops Lane 9 H3K4me3 Lane 10 H3R8me1 Properties Lane 11 Lane 12 H3R8me2 (asy) Lane 13 H3R2un

Storage Store at -20 °C. Avoid freeze / thaw cycles. WB Stability Stable for 12 months from date of receipt / reconstitution Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Constituents PBS, Glycerol, BSA Primary ab incubation condition: 2 hours at room temperature Purity Antibodies are purified by protein G-conjugated agarose Secondary ab: Goat Anti-Mouse IgG H&L followed by di-methylated(sy) (Arg2) peptide (HRP) Lysate: MCF-7, BRL, COS-7 affinity chromatography. Protein loading quantity: 20 μg Isotype IgG Exposure time: 60 s 17 kDa Predicted MW: Target Information Observed MW: 17 kDa

Function Histone post-translational modifications (PTMs) are key

mechanisms of that modulate Research Use

structures, termed as “”. The PTMs on For research use only, not for use in diagnostic procedures. histone including acetylation, , phosphorylation and novel acylations directly affect the

accessibility of chromatin to transcription factors and other

epigenetic regulators, altering genome stability, gene

transcription, etc. occurs primarily at

lysine and arginine residues on the amino terminal of core

. Methylation of histones can either increase or

decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated

and how many methyl groups are attached (mono-, di-, tri-

methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs

primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20,

while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs)

and histone demethylases (HDMs) are major regulating

factors. Cellular location Nucleus

APPLICATIONS WB: Western blot IP: CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] MODIFICATIONS ac: acetylated bhb: β-hydroxybutyrylated bu: butyrylated bz: benzoylated cr: crotonylated hib: 2-hydroxyisobutyrylated la: lactylated Web: www.ptm-biolab.com.cn www.ptmbiolabs.com pr: propionylated su: succinylated un: unmodified