Anti-Symmetric Di-Methyl-Histone H3 (Arg2) Mouse mAb Catalog # PTM-693 General Information Images Host species Mouse Dot Blot Clonality Monoclonal Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Clone number / Primary ab incubation condition: 2 hours at room temperature Synonym H3R2me2 (sy) Secondary ab: Goat Anti-Mouse IgG H&L Immunogen UniProt MW (kDa) Applications (HRP) species Immunogen peptide quantity: 1 ng, 4 ng, 16 ng Human P68431 17 WB Exposure time: 60 s The list of peptides is included in the table below: No. Peptide No. Peptide Product Usage Information Lane 1 H3R2me2 (sy) Lane 2 H3R2me1 Application Dilution Recommended species Lane 3 H3R2me2 (asy) Lane 4 H3K4ac Human, Mouse, Rat, Lane 5 H3K4cr Lane 6 H3K4hib WB 1:500-1:2000 Lane 7 H3K4me1 Lane 8 H3K4me2 Chlorocebus aethiops Lane 9 H3K4me3 Lane 10 H3R8me1 Lane 11 H3R8me2 Lane 12 H3R8me2 (asy) Properties Lane 13 H3R2un Storage Store at -20 °C. Avoid freeze / thaw cycles. WB Blocking buffer: 5% NFDM/TBST Stability Stable for 12 months from date of receipt / reconstitution Primary ab dilution: 1:2000 Constituents PBS, Glycerol, BSA Primary ab incubation condition: 2 hours at room temperature Purity Antibodies are purified by protein G-conjugated agarose Secondary ab: Goat Anti-Mouse IgG H&L followed by di-methylated(sy) histone H3 (Arg2) peptide (HRP) Lysate: MCF-7, BRL, COS-7 affinity chromatography. Protein loading quantity: 20 μg Isotype IgG Exposure time: 60 s Predicted MW: 17 kDa Target Information Observed MW: 17 kDa Function Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin Research Use structures, termed as “histone code”. The PTMs on For research use only, not for use in diagnostic procedures. histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri- methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] MODIFICATIONS ac: acetylated bhb: β-hydroxybutyrylated bu: butyrylated bz: benzoylated cr: crotonylated hib: 2-hydroxyisobutyrylated la: lactylated Web: www.ptm-biolab.com.cn www.ptmbiolabs.com pr: propionylated su: succinylated un: unmodified .