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Temas E Sessões Internas ACTAS DE BIOQUÍMICA VOLUME 5 TEMAS E SESSÕES INTERNAS Editors J. Martins e Silva Carlota Saldanha 1991 “Actas de Bioquímica ” is primarily concerned with the publication of complete transcripts of the lectures, conferences and other studies presented in Advanced Post-Graduate Courses or in Scientific Symposia organized or co-organized by the Instituto de Bioquímica, Faculdade de Medicina da Universidade de Lisboa. Original or review articles and other scientific contributions in askin fields may also be included. Editors J. Martins e Silva Carlota Saldanha Editorial Office Instituto de Bioquímica, Faculdade de Medicina da Universidade de Lisboa Av. Prof. Egas Moniz Lisboa – Portugal Mailing Address Actas de Bioquímica Apartado 4098 1500-001 Lisboa – Portugal Subscription Information Subscription price is $25.00 (twenty five US dollars) (twenty euros) per volume. An additional charge of $5,00 per volume is requested for post delivery outside Portugal. Payment should accompany all orders. Correspondence concerning subscription should be addressed to the mailing address above. ISBN: 972-590-076-6 Copyrigh@ 1991 by Instituto de Bioquímica, Faculdade de Medicina da Universidade de Lisboa. All rights reserved. Printed in Portugal II ACTAS DE BIOQUÍMICA VOLUME 5 1991 ÍNDICE PREFÁCIO .................................................................................................................................................................................................................................................... V ARTIGOS Liposomes and their application to the study of cell membrane fusion F.M. Goñi ......................................................................................................................................................................................................................................... 3 Isolation of calmodulin - binding proteins from skeletal muscle sarcoplasmic reticulum M. Graça P. Vale .................................................................................................................................................................................................................. 15 Differentiation between two targets for the action of compound 48/80 on the Ca 2+ uptake by sarcoplasmic reticulum M. Graça P. Vale, M. Manuel R. Donato .................................................................................................................................................... 25 SESSÕES INTERNAS DO INSTITUTO 1987 Métodos de extracção e separação de lípidos da membrana – acção dos detergentes Yolanda Pinto .................................................................................................................................................................................................................................... 43 Metodologia e resultados obtidos com técnicas de doseamento do plasminogénio, alfa 2-antiplasmina e antitrombina III Luís Cardoso ........................................................................................................................................................................................................................................ 51 O 2,3-bisfosfoglicerato Maria Manuela Nunes ............................................................................................................................................................................................................ 57 Liposomas: princípios teóricos e metodologia Yolanda Pinto ...................................................................................................................................................................................................................................... 61 1988 Diabetes e envelhecimento Mário Carreira .................................................................................................................................................................................................................................. 71 Dislipidémias e obesidade Leonor Queiroz ................................................................................................................................................................................................................................. 75 III ACTAS DE BIOQUÍMICA VOLUME 5 1991 Respiração mitocondrial J.P. Guimarães .................................................................................................................................................................................................................................. 79 Organofosforados, seus malefícios Filomena Dias Alves .................................................................................................................................................................................................................. 85 Proteínas C e S Luís Cardoso ......................................................................................................................................................................................................................................... 89 Colinesterases e paratião Teresa Luísa Quintão ............................................................................................................................................................................................................... 95 Purificação de proteínas J. Rodrigues Loureiro .............................................................................................................................................................................................................. 97 Transporte iónico no eritrocito; importância fisiológica do permutador Na +/H + M. Manuela Nunes ....................................................................................................................................................................................................................... 111 Regulação do cálcio intracelular Yolanda Pinto ...................................................................................................................................................................................................................................... 115 Alterações no 2,3-DPG (2,3-difosfoglicerato) em sangue armazenado com implicações na deformabilidade eritrocitária M. Manuela Nunes ....................................................................................................................................................................................................................... 125 Cromatografia líquida de alta resolução (HPLC) Teresa Quintão .................................................................................................................................................................................................................................. 129 Factores bioquímicos da migração neoplásica Mario Carreira .................................................................................................................................................................................................................................. 133 Importância dos grupos sulfidrílicos da membrana eritrocitária na hemólise Filomena Alves .................................................................................................................................................................................................................................. 139 1989 Mecanismos reguladores da comunicação endotélio-neurónio no cérebro João Paulo Guimarães ........................................................................................................................................................................................................... 141 Acção dos detergentes na membrana eritrocitária Yolanda Pinto ...................................................................................................................................................................................................................................... 149 Interacções globulares na agregação eritrocitária Luís Cardoso ......................................................................................................................................................................................................................................... 155 Transporte de água transmembrana eritrocitária Manuela Nunes .................................................................................................................................................................................................................................. 159 IV ACTAS DE BIOQUÍMICA VOLUME 5 1991 PREFÁCIO Temos a maior satisfação em acolher neste número de Actas de Bioquímica três importantes artigos de revisão sob diferentes aspectos da bioquímica das membranas. No primeiro desses trabalhos, o Professor Goñi (da Faculdade de Ciências da Universidade do País Basco), demonstra o comportamento de diferentes tipos de fosfolípidos em meio aquoso e a sua importância ao formarem vesículas, como modelos de estudo membranar. Os outros dois trabalhos, da Professora Maria da Graça Vale (da Faculdade de Ciências da Universidade de Coimbra), abordam a modulação do transporte de cálcio através da membrana sarcoplámica na dependência da actividade da ATPase Ca 2+ ou da actividade da calmodulina. Incluem-se também os extractos de comunicações apresentadas por alguns dos docentes e colaboradores voluntários do Instituto de Bioquímicas, nas reuniões
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