Authors' pre-print version.

Manuscript published in Molecular Biology and Evolution MBE-13-0254.R2

DOI of published manuscript: 10.1093/molbev/mst164

Article Discoveries Section

Distribution and Evolution of the Mobile vma-1b Intein.

Kristen S. Swithers1,3, Shannon M. Soucy1, Erica Lasek-Nesselquist1,5, Pascal Lapierre2,4 and Johann Peter Gogarten1*

1 Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, United States of America

2 University of Connecticut Biotechnology Center, University of Connecticut, Storrs, Connecticut, United States of America 3 Current affiliation: Department of Cell Biology, Yale University Medical School, New Haven, Connecticut, United States of America 4 Current affiliation: New York State Department of Health, Wadsworth Center, Albany, New York, United States of America 5 Current affiliation: Department of Biology, University of Scranton, Scranton, PA, United States of America Communicating author: Johann Peter Gogarten Communicating author email: [email protected], [email protected]

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Abstract

Inteins are self-splicing parasitic genetic elements found in all domains of life. These genetic elements are found in highly conserved positions in conserved proteins. One protein family that has been invaded by inteins is the vacuolar and archaeal catalytic ATPase subunits (vma-1). There are two intein insertion sites in this protein, "a" and "b". The "b" site was previously thought to be only invaded in archaeal lineages. Here we survey the distribution and evolutionary histories of the "b" site inteins and show the intein is present in more lineages than previously annotated including a bacterial lineage, Mahalla australienses 50-1 BON. We present evidence, through ancestral character state reconstruction and substitution ratios between host genes and inteins, for several transfers of this intein between divergent species, including an interdomain transfer between the Archaea and Bacteria. While inteins may persist within a single population or species for long periods of time, transfer of the vma-1b intein between divergent species contributed to the distribution of this intein.

Keywords: intein, parasitic gene, homing endonuclease, gene transfer, co-evolution of genes

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Introduction

Inteins are self-splicing, parasitic, mobile genetic elements that splice out of their flanking proteins sequence (extein) after translation via an autocatalytic mechanism (Cooper and Stevens 1995; Perler et al. 1994). Inteins can be divided into two different groups based on their size - large and mini inteins - which are differentiated based on the presence or absence of a homing endonuclease (HE) domain (Elleuche and Poggeler 2010; Liu 2000). The large inteins contain a HE domain, an enzyme with endonuclease activity that has a 14 – 40 bp recognition site (Chevalier and Stoddard 2001), and N- and C-terminal splicing domains (Pietrokovski 1998). This HE domain provides mobility to the intein and the ability to move into new intein-less target sites through a process called homing (Chevalier and Stoddard 2001; Gogarten and Hilario 2006). Transfer of the intein to hosts that contain an intein-less target site is necessary for the HE to remain under purifying selection (Goddard and Burt 1999). The transfers of the HE containing inteins can occur either within or between species (Barzel et al. 2011; Clerissi et al. 2013; Gogarten and Hilario 2006; Koufopanou et al. 2002; Macgregor et al. 2013; Okuda et al. 2003; Yahara et al. 2009). The mini intein is simply composed of the N and C-terminal splicing domains with a linker region between them. Inteins are found in all three domains of life and in viruses, and tend to be present in conserved regions of proteins (exteins) with high sequence similarity (Swithers et al. 2009). Targeting conserved sites in conserved proteins is advantageous for two reasons. First, it provides selective pressure on the splicing domains, which ensures the intein is spliced out exactly to maintain a functional extein. Mutations to the splicing domains of the intein will result in improper splicing, resulting in a nonfunctional extein that could be detrimental to the cell. Second, targeting the most conserved regions of the most conserved genes will facilitate transfer to inteinless alleles across species or even domain boundaries. One such conserved protein family invaded by inteins is the vacuolar and archaeal catalytic ATPase subunits (vma-1) (Hirata et al. 1990; Kane et al. 1990; Senejani et al. 2001). The vacuolar, archaeal, and bacterial ATPases (or ATP synthases) are a family of multi-subunit proteins that are present in all three domains of life and share a common ancestor (Gogarten et al. 1989; Zimniak et al. 1988). The eukaryotic version, called the vacuolar ATPase (V-type), is involved in the intravesicular acidification of the endomembrane system (vacuoles, lysosome, endosomes and trans Golgi network) and it is also found in the plasma membrane of specialized cells of transport epithelia (Harvey and Nelson 1992). The archaeal (A-type) and bacterial (F-type) counterparts are used for ATP generation and/or ion transport. Several cases of horizontal gene transfer of the A-type ATPase to Bacteria have been documented (Hilario and Gogarten 1993; Lapierre 2007; Lapierre et al. 2006). Two distinct sites in this family of ATPases have been invaded by inteins; the “a” site in the vacuolar ATPases in yeasts and the “b” site in the Archaea. These two insertion sites have been shown to be in the most conserved parts of the protein (Swithers et al. 2009). The intein in the "a" site was frequently transferred between different yeast species (Koufopanou et al. 2002; Okuda et al. 2003). The intein in the "b" site has a wide phylogenetic distribution among Archaea. To date the intein database (InBase) annotates seven vma-1b inteins in the Thermoplasma and Pyrococcus (Perler 2002) genera. Here we report on additional vma-1b inteins, show that they are found in more diverse species, and discuss their evolutionary histories.

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Table 1. Distribution of vma-1b inteins.

AA Organisms Lineage Length Isolated From Reference of Intein

Shallow marine solfatara at Pyrococcus furiosus DSM Archaea; Euryarchaeota; (Fiala and Stetter 426 Vulcano Island off southern 3638 Thermococci 1986) Italy

Archaea; Euryarchaeota; Hydrothermal Vent in the North Pyrococcus abyssi GE5 428 (Erauso et al. 1993) Thermococci Fiji Basin in the Pacific Ocean

Hydrothermal vent in Papua Archaea; Euryarchaeota; Pyrococcus sp. NA2 428 New Guinea-Australia-Canada- (Lee et al. 2011) Thermococci Manus (PACMANUS) field

Hydrothermal vent in the Archaea; Euryarchaeota; (Gonzalez et al. Pyrococcus horikoshii OT3 375 Okinawa Trough in the Pacific Thermococci 1998) Ocean

Thermococcus litoralis Archaea; Euryarchaeota; Shallow submarine hot spring at 428 (Neuner et al. 1990) DSM 5473 Thermococci Lucrino Beach near Naples

Thermoplasma Self-heating coal refuse pile (Searcy 1975; Archaea; Euryarchaeota; acidophilum 122-1B2 174 from the Friar Tuck mine in Senejani et al. Thermoplasmata ATCC 25905 Indiana, USA 2001)

Self-heating coal refuse pile Thermoplasma Archaea; Euryarchaeota; (Darland et al. 173 from the Friar Tuck mine in acidophilum DSM 1728 Thermoplasmata 1970) Indiana, USA

Thermoplasma volcanium Archaea; Euryarchaeota; 185 Solfataric field (Segerer et al. 1988) GSS1 Thermoplasmata

Ultraback A location (UBA site) Thermoplasmatales Archaea; Euryarchaeota; 179 in the Richmond Mine, Iron (Dick et al. 2009) archaeon I-plasma Thermoplasmata Mountain, CA

5-way CG acid mine drainage Archaea; Euryarchaeota; Ferroplasma sp. Type II 535 site in the Richmond Mine, Iron (Tyson et al. 2004) Thermoplasmata Mountain, CA

Picrophilus torridus DSM Archaea; Euryarchaeota; Dry solfataric field in northern (Schleper et al. 332 9790 Thermoplasmata Japan 1995)

Methanothermus fervidus Archaea; Euryarchaeota; Hot solfataric spring from (Anderson et al. 357 DSM 2088 Methanobacteria Iceland 2010)

Candidatus Nanosalinarum Archaea; Euryarchaeota; Hypersaline lake NW Victoria, (Narasingarao et al. 522 sp. J07AB56 Nanohaloarchaea Australia 2012)

Metagenome Deep-sea hypersaline anoxic Thetis Sea contig00086 521 (Ferrer et al. 2012) gi number 3548555569 lake Thetis

Bacteria; Firmicutes; Clo Mahella australiensis 50-1 Riverslea oil field in the Bowen- stridia; Thermoanaerobac 522 (Salinas et al. 2004) BON Surat basin, Australia terales

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Results

Distribution of vma-1b inteins. Databases at the NCBI were surveyed for vma-1 exteins and inteins that reside in the b insertion position (vma-1b). To date we identified fifteen vma-1b inteins within complete flanking extein sequences present in these databases (Table 1). Thirteen are found within the Euryarchaeota, one is present in Clostridia and another is found in a deep sea hypersaline metagenomic sequencing project. Additionally, we identified two inteins in contigs from a marine metagenome (gi:129952466) and a hot springs metagenome (gi:290482983) that only encode partial extein sequences. These partial sequences did not contain sufficient information for phylogenetic placement of the host organisms. The multiple sequence alignment shows the vma-1b intein family is composed of both mini inteins and larger inteins with homing endonuclease domains (see supplementary materials, alignment of intein sequences). The larger inteins all have the LAGLIDADG homing endonuclease domains, suggesting these may be active homing endonucleases. However, additional experimental evidence is required to verify their activity. Compared to the currently annotated inteins in InBase, we report an additional ten inteins at this insertion site in three new classes of prokaryotes. Breakpoints within the extein/intein alignment. The genetic algorithm for recombination detection (GARD) determined breakpoints in the extein/intein alignment. This algorithm analyzes fragments of the overall alignment and compares the goodness of fit of phylogenies from these smaller alignments under a maximum likelihood framework using the corrected Akaike Information Criterion. Significant breakpoints were identified three amino acids before the insertion site (position 245 p<0.01) and at three amino acids before the end of the intein (position 891 p<0.01) (Figure S1). The fact that the GARD algorithm detects breakpoints three amino acids upstream of the insertion site might be due to the conserved, phylogenetically uninformative nature of the splice site. The last three amino acids at the carboxy terminal end of the intein are conserved in all the inteins included in this study. This level of conservation is more typical for the extein than for the intein sequences (see histograms for conservation in Figure S8). Regardless of the slight misplacement of the breakpoint, the GARD analysis does suggest that extein and intein have different evolutionary histories, which is also corroborated by the intein and extein trees (cf. Figure 2). Archaeal-type ATPase distribution. A survey of the bacterial domain reveals that all major clades of Bacteria have representatives containing an archaeal-type ATPase (uninvaded extein sequence). A phylogenetic analysis of the extein protein using representatives from all clades shows three bacterial clusters interspersed within the archaea (Supplementary Figure S2). The phylogeny of the archaeal exteins is in good agreement with the ribosomal protein phylogeny (see below). Thus, the finding that the Bacteria are interspersed within the Archaea suggests that the three distinct bacterial groups (Supplementary Figure S2) represent independent gene transfer events from Archaea to Bacteria.

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2558 6 a Del t 3322 3

r t s CC m AT CC T u A 1 lea t 2073 1 350 2 1157 1 2C P rophicus 1

2740 5 nu c t

D SM 1226 1 o 208 8 M 216 0 p CC O D S SM 172 8 DSM T

1679 0 hanolicus CC K D e D A T um Z 730 3

7 SM 551 1 1 NA 1 r A 2 t DSM 6 B 363 8 D DSM SM 430 4 ans 3

SM 1294 0 s B SK3 6 S

D iga t DSM a m s ub AV1 9 H SM 1709 3 bien s A u AB 5 s D an s R pseude t i DSM er t hermau gali B s 501 BO N x D i lea t roleariu s er ermen t eves t idophilu m r dehalogenan s J 07 c ctr i rkm eni c i walsbyi pe t vannielii a nu c ulinum onnurineus pharaonis ahen s kodakarensis u kandleri

sanguinis t s jeo t u m s p iu m c olo um bo t pe rfr ingen s SM 10 1 r u s r adiodu cc u adio v o hermobac t hermus f ervidus au str alien s cc u s t he rm ophilu myx oba ct e o rr igena t alina r ali c ococcus o habdu s e ridium ridium t hermocellum Min e osa lin a hanohalobium hano t hanopyrus hano t oba ct eriu m hanoplanu s hanococcus ronomonas t n s rep t e ahella a nae r t he rm u he rm opla sm a u ruepera r hermoanaerobac t hermococcus hermococcus Leptotrichia buccalis DSM 113 5 Streptobacillus moniliformis DSM 1211 2 F Methanocaldococcus vulcanius M 7 Methanocaldococcus infernus M E Coprothermobacter proteolyticus DSM 526 5 Dictyoglomus turgidum DSM 672 4 Dictyoglomus thermophilum H61 2 Thermosediminibacter oceani DSM 1664 6 T Methanosphaera stadtmanae DSM 309 1 Methanobrevibacter ruminantium M 1 Methanobrevibacter smithii ATCC 3506 1 Methanococcus aeolicus Nankai 3 Methanococcus voltae A 3 Methanococcus maripaludis C 6 Me t Clos t Finegoldia magna ATCC 2932 8 Anaerococcus prevotii DSM 2054 8 Clostridium cellulovorans 743 B Enterococcus faecalis V58 3 S Streptococcus pyogenes M1 GA S Clostridium difficile 63 0 Clostridium phytofermentans ISD g Clostridium tetani E8 8 Me t Streptococcus gallolyticus UCN3 4 Streptococcus pneumoniae TIGR 4 Streptococcus pneumoniae CGSP1 4 Streptococcus mitis B 6 Clostridium botulinum E3 str Alaska E4 3 Clo str idiu m Clostridium novyi N T Clos t Ferroplasma acidarmanu s Thermoplasma volcanium GSS 1 Thermoplasma acidophilum 1221B 2 T M Acholeplasma laidlawii PG8 A Eubacterium limosum KIST61 2 Acidaminococcus f Thermococcus sibiricus MM 73 9 Thermococcus litoralis DSM 547 3 Me t Thermanaerovibrio acidaminovorans DSM 658 9 Meiothermus ruber DSM 127 9 Acid Thermoplasmatales archaeo n Picrophilus torridus DSM 979 0 Ferroplasma acidarmanus fer 1 Micrarchaeu m Parvarchaeu m N Halobacterium salinarum R 1 Methanosaeta thermophila P T Methanosarcina barkeri str Fusar o Methanosarcina acetivorans C2 A Methanosarcina mazei Go 1 Me t Methanococcoides burtonii DSM 624 2 Methanohalophilus mahii DSM 521 9 Methanoculleus marisnigri JR 1 Thermococcus gammatolerans EJ 3 Pyrococcus yayanosi i Pyrococcus f uriosus Pyrococcus sp NA 2 Pyrococcus horikoshii OT 3 Pyrococcus abyssi GE 5 Aminobacterium colombiense DSM 1226 1 Methanocella paludicola SANA E Methanospirillum hungatei JF 1 Am inoba ct e Geobacter uraniireducens Rf 4 A T Deino c T Nano s Halomicrobium mukohataei DSM 1228 6 Haloarcula marismortui ATCC 4304 9 Halal k Candidatus Methanosphaerula palustris E19 c Candidatus Methanoregula boonei 6A 8 Methanocorpusculum labreanum Z M Me t Thetis Se a T T Anaeromyxobacter sp Fw109 5 Haloquadra t Halo r Methanospirillum hungatei JF 1 Halorubrum lacusprofundi ATCC 4923 9 Haloferax volcanii DS 2 Na t Natrialba magadii ATCC 4309 9 Halo t uncultured methanogenic archaeon RC I Methanocella paludicola SANA E Ferroglobus placidus DSM 1064 2 Arc haeoglobu s f ulgidu Archaeoglobus profundus DSM 563 1

Euryarchaeota Actinobacteria Firmicutes Deltaproteobacteria Dictyglomi Deinococcus-Thermus

Figure 1. Maximum Parsimony Ancestral Character State Reconstruction. The depicted cladogram represents the part of the VMA-1 tree where inteins are present (see Supplemental Figure S2 for full tree). Presence and absences of the intein are represented at the tips of the tree; black circles correspond to presence of the intein and white circles correspond to absences of the intein. The single most parsimonious reconstruction with nine steps indicated seven independent gains and two losses of the intein. The gains are once for the Thetis sea sequence, once for the Pyrococcus spp., once for the Thermococcus litoralis, once for Methanothermus fervidus, once for Mahella australiensis, once for the Thermoplasmatales and once for Candidatus Nanosalinarum sp. J07AB56. The two losses are in the Acid Mine sequence and Ferroplasma acidarium fer1. A maximum likelihood reconstruction using the MK1 model (Lewis 2001) produced nearly identical results (see supplemental figure S3). Archaeal extein and ribosome trees. Overall, the extein and ribosomal protein reference phylogeny display a high degree of topological similarity, particularly at the ordinal level. Many clades show identical branching orders, such as the Methanosarcinales, Methanococcales, and Methanobacteriales (Supplementary Figure S4). Deeper phylogenetic relationships were also similar, including the sister relationship between the Archaeoglobi and a Halobacteria-Methanomicrobia clade. Less resolved positions on the tree tended to stem from taxa on long branches, prone to artifact, such as Micrarchaeum and Nitrosopumilis in the ribosomal phylogeny. The reduced extein and ribosomal protein trees were congruent (Supplementary Figure S5) with the exception of the “misplacement” of Candidatus Nanosalinarum sp. in the ribosome phylogeny due to LBA. Both SH and AU tests indicated that the extein and ribosomal topologies were not significantly different from each other (p-values = 0.28 and 0.73 for AU and SH tests, respectively). This suggests that the archaeal exteins follow ribosomal evolution (considered to be vertical evolution) and have not been transferred between divergent archaeal lineages. This finding also implies that transfers of the intein occurred independently of any extein transfers. !"("!"

Ancestral Character State Reconstruction. Maximum parsimony and maximum likelihood ancestral state reconstructions (ASR) were performed using the phyletic patterning of the intein relative to its host gene across the prokaryotes (Figure 1). There was only one most parsimonious reconstruction with nine steps given the dataset, which is validated by the maximum likelihood (ml) ASR analysis. The reconstructions suggest seven independent gains and two losses of the intein. The reconstructions suggest seven independent gains and two losses of the intein. According to the parsimony and the ml reconstruction the Thetis sea sequence (probability from mlASR P=100%), the ancestor of the four Pyrococcus spp. (P=99%), T. litoralis (P=100%), M. australienses (P=100%), Candidatus Nanosalarium (P=100%), M. fervidus (P=100%) and the ancestor of the Thermoplasma/Ferroplasma/Picrophilus/Thermoplasmatales clade (P=70%) all independently gained the intein. See the discussion section for consideration of the overestimation of intein absence in the species represented by the leaves of the cladogram in Figure 1.

Thermoplasmatales archaeon I-plasma Thermoplasmatales archaeon I-plasma

0.99/0.24/49 1/0.89/74 Extein Thermoplasma volcanium GSS1 Thermoplasma volcanium GSS1 Intein

1/1/100 1/0.92/91 Thermoplasma acidophilum DSM 1728 Thermoplasma acidophilum DSM 1728 1/1/100 1/1/100 1/1/100

Thermoplasma acidophilum 122-1B2 Thermoplasma acidophilum 122-1B2 1/0.94/72

Picrophilus torridus DSM 9790 Picrophilus torridus DSM 9790 1/0.98/96 1/1/100 1/1/99

Ferroplasma sp. Type II Ferroplasma sp. Type II 0.97/0.02/44 1/1/100 1/1/100 Mahella australiensis 50-1BON Mahella australiensis 50-1BON

0.73/0.06/49 Methanothermus fervidus DSM 2088 Methanothermus fervidus DSM 2088

1/0.69/73 Candidatus Nanosalinarum sp. J07AB56 Candidatus Nanosalinarum sp. J07AB56 1/1/100 1/0.97/96 Thetis Sea contig00086 Thetis Sea contig00086

Thermococcus litoralis DSM 5473 Pyrococcus sp. NA2 0.99/0.32/58 Pyrococcus furiosus DSM 3638 Pyrococcus abyssi GE3 1/1/100 0.94/0.32/45 1/1/100 Pyrococcus abyssi GE3 Thermococcus litoralis DSM 5473 0.91/0.56/39 0.61/0.23/51 Pyrococcus horikoshii OT3 Pyrococcus furiosus DSM 3638 1/0.97/89 1/0.97/98

Pyrococcus sp. NA2 Pyrococcus horikoshii OT3 0.2 0.3

Figure 2. Extein and Intein Phylogenetic Trees. Phylogenetic trees depicting the extein (left) and intein (right). Support values (from left to right) were determined by posterior probability, approximate likelihood ratio test, and bootstrap replicates. Supported incongruencies are found between the extein and intein tree in the Pyrococcus/Thermococcus clade. Multiple horizontal gene transfers of the vma-1b intein. Phylogenetic reconstructions were preformed for the extein and intein splicing domains. These trees are incongruent with each other by both the AU and SH tests (p!0.01). This significant incongruence is due to a well-supported transfer of the intein from within the Pyrococcus spp. to T. litorialis (Figure 2). Unfortunately, none of the other discrepancies between the extein and intein trees show high bootstrap support. Including the HE domain in reconstruction of the intein's phylogeny did not improve the placement of the bacterial intein sequence from Mahella australiensis relative to the Methanothermus and Picrophilus/Ferroplasma inteins (Supplementary Figure S6). The ratio of extein to intein substitution rates provides additional insight on intein transfers relative to exteins. Differences in divergence rates (Novichkov et al. 2004) and higher than expected sequence similarity (e.g., Podell and Gaasterland 2007) are frequently used to infer HGT events. Immediately after an intein !")"!" has been transferred between two species, the intein sequences are identical between donor and recipient and more conserved than the extein. This unexpected high sequence similarity of two inteins is an indcation of transfer of an intein (Liu et al. 1997). Moreover, the extein and intein evolve under very different selection pressures. While the ATPase catalytic subunit belongs to one of the most conserved protein families (Gogarten 1994; Swithers et al. 2009), the inteins evolve much faster making sequence based phylogenetic reconstruction difficult (Gogarten et al. 2002; Perler et al. 1997). This is reflected in the conservation profiles of the extein and intein (Supplemental Figure S7). The intein has a significantly higher average sequence variation score than the extein, 3.42 and 2.85 respectively (p<0.01, T-test). The higher score also indicates a higher substitution rate, because it represents the number of different amino acids in a sliding window. Among site rate variation for the extein sequences is more extreme than for the intein sequences, i.e. the exteins contain many sites under strong purifying selection (Supplemental Figure S8). To assess the divergence rate of the inteins relative to extein sequences, we used the well-aligned splicing domains of the intein, omitting the less conserved linker and homing endonuclease domains (see Alignments, Supplementary Material). The phylogenetic reconstructions of the extein and intein datasets are the same for the Thermoplasma genus and the Thermoplasmatales I- plasma archaeon, suggesting that in this group (in the following designated as Thermoplasma group) the extein and intein were inherited together. The inteins in these sequences (Table 1) also have lost their HE domain, which suggests they are no longer mobile and further strengthens the notion that the intein was inherited together with the extein for this clade. Therefore, the pairwise ratios of the substitution rates of extein to intein in this clade should represent ratios expected under co-inheritance of extein and intein. Ratios significantly greater than these (i.e. the intein is less divergent than expected), likely indicates horizontal transfer of the intein relative to the extein (see Table 2 for ratios). The chosen cut- off level of 0.7 corresponds to a significance level of p<0.002 given the rate ratios within the Thermoplasma group. To assess false positive rates obtained using this ratio approach we simulated sequence evolution along the extein tree using the parameters estimated for the intein and extein sequences. Using the rate ratio of 0.31, corresponding to the ratio of the lengths of intein and extein subtree in the Thermoplasma group, the rate of false positives was smaller than 0.000005. Incorporating uncertainty of the extein/intein ratio into the simulation, the false positive rate increases to 0.0025, corresponding to an expectation of 0.5 false identifications of transfer in Table 2. The most parsimonious explanation for the ratios is mapped on the extein phylogeny in Figure 3. Although these values cannot resolve direction of transfer they do show the vma-1b inteins are mobile genetic elements that have undergone several transfers throughout the evolution of the gene family encoding the archaeal ATPase catalytic subunit, including several transfers between divergent organisms.

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Table 2. Pairwise Extein to Intein Substitution Ratios.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 1 Thermoplasmatales archaeon I-plasma 2 Thermoplasma volcanium GSS1 0.39 3 T. acidophilim DSM 1728 0.35 0.14 4 T. acidophilum DSM 122-1B2 0.39 0.17 0.53 5 Feroplasma acidarmanus Type II 0.49 0.40 0.37 0.41 6 Picrophilus torridus DSM 9790 0.56 0.33 0.44 0.48 0.63 7 Mahella australiensis 50-1 BON 0.55 0.44 0.42 0.46 0.75 0.73 8 Candidatus Nanosalinarum so. J07AB56 0.59 0.57 0.59 0.72 0.71 0.75 0.64 9 Thetis_Sea_contig_00086 0.60 0.51 0.49 0.58 0.60 0.59 0.56 1.22 10 Methanothermus fervidus DSM 2088 0.71 0.50 0.48 0.50 0.86 0.84 0.74 0.48 0.37 11 Thermococcus litoralis DSM 5473 0.45 0.37 0.31 0.34 0.53 0.44 0.34 0.48 0.42 0.32 12 Pyrococcus abyssi GE5 0.48 0.38 0.32 0.35 0.54 0.42 0.36 0.53 0.47 0.38 0.41 13 Pyrococcus sp. NA2 0.47 0.38 0.33 0.36 0.51 0.42 0.35 0.53 0.46 0.36 0.41 0.50 14 P. furiosus DSM 3638 0.45 0.38 0.32 0.35 0.53 0.44 0.37 0.53 0.45 0.34 1.29 0.27 0.30 15 P. horikoshii OT3 0.43 0.38 0.31 0.35 0.55 0.42 0.35 0.50 0.43 0.32 0.76 0.09 0.13 0.59

Number of amino acid substitutions were calculated for extein and intein sequences and corrected for multiple substitutions. Ratios greater than inside the Thermoplasma group (the top four entries in the table) are taken to be deviations from a ratio of extein to intein that represents co-inheritance of intein and extein. Bold font indicates ratios significantly greater than the ones within the Thermoplasma group.

Figure 3. Pairwise substitution rate ratios mapped onto the extein tree. Lines connect taxa with extein to intein substitution ratios greater than 0.7. Although direction of intein movement cannot be determined using these ratios, the ratios reveal several putative transfers of the vma-1b intein. !"+,"!"

Discussion

Compared to the currently annotated sequences in InBase we have identified ten additional inteins belonging to the vma-1b family of inteins. Three of which are in new taxonomic classes that were not previously described, the Methanobacteria, Nanohaloarchaea, and Clostridia. This is the first time the vma-1b intein is found in a bacterial lineage. Similar to the PRP8 inteins (Bokor et al. 2012) the vma-1b family of inteins is composed of mini inteins and inteins with homing endonuclease domains. These likely represent inteins in different states of the homing or life-cycle. In the homing cycle an exogenous intein with a homing endonuclease comes in contact with a population that does not contain an intein and spreads through the population through super-Mendelian rates of inheritance (Goddard and Burt 1999; Gogarten and Hilario 2006; Gogarten et al. 2002). Once all target sites in a population are occupied by inteins containing HEs there is reduced selection on the HE and it starts to degrade over time, followed by loss of the intein. The mini inteins of the Thermoplasmatales order may be representatives of the later part of the homing cycle, indicating a more ancient invasion of the vma-1b site in this clade. Even in cases where the homing cycle does not go to complete fixation in the intein containing allele, the individual intein insertion sites progress through the same life history from empty target site, occupation by an intein with functioning HE, and then eventual decay of the HE activity (Barzel et al. 2011; Yahara et al. 2009). The evolutionary history of the vacuolar, archaeal and bacterial ATPase catalytic subunit is characterized by several gene transfer events, including transfers between the archaeal and the bacterial domain. Due to the sequence conservation of the catalytic ATPase subunit, and because the ATPase phylogeny can be rooted by an ancient gene duplication event (Gogarten et al. 1989), these transfer events have been well established (Hilario and Gogarten 1993; Lapierre 2007; Lapierre et al. 2006; Olendzenski et al. 2000). Although there were multiple transfers of the host gene, all of our analyses were preformed relative to the host gene in order to distinguish the intein transfers from the transfers of the extein. Breakpoint analyses, consideration of pairwise substitution rates, and ancestral character state reconstructions all suggest that the inteins repeatedly invaded the vma-1 host gene in independent transfer events. In particular these phylogenetic methods suggest that the intein in Mahella australiensis was gained independently from the between domain transfer of the host A-ATPase operon. The alternate explanation that the intein was gained only once in the ancestor of the vma-1 protein family and that the extant presence/absence pattern is a result of vertical descent and loss of the intein is incompatible with the above listed evidence. In addition, under the loss-only scenario there would have to be a minimum of 28 steps to explain the patterning, one gain and 27 losses, opposed to only nine steps to explain the intein distribution through independent gains and losses of the intein. The scenario with the minimum number of events is depicted in Figure 1. It involves seven intein gains and two losses. Study of inteins and homing endonucleases in natural populations as well as theoretical considerations suggest that alleles with and without inteins can coexist in populations for long periods of time (Barzel et al. 2011; Butler et al. 2006; Gogarten and Hilario 2006; Yahara et al. 2009). Given that the sequences used in this study only represent one member of a larger population, assigning a species branch a character state of intein absence ignores the possibility that other members of the species might contain the intein. Thus, only taking into consideration the ASR, one needs to entertain the possibility that the loss events !"++"!" inferred for branches represent only absence in the particular sampled genome, and not absence in the population or species. Likewise, the inference that the ancestor of the Pyrococcus and Thermococcus genera did not harbor the intein is not strongly supported through the parsimony reconstruction. The most parsimonious scenario depicted in figure 1 corresponds to two gains; the presence of the intein in the Pyrococcus - Thermococcus ancestor corresponds to three loss events within the Pyrococcus and Thermococcus group. Given that intein absence possibly is overestimated, three apparent loss events versus two gains do not provide a strong argument for intein gain in T. litoralis. However, the gain of the intein in T. litoralis is also supported by the phylogeny of the intein-splicing domain, which groups T. litoralis inside the cluster of homologs from the Pyrococcus species, supporting the hypothesis that the intein in T. litoralis was recently acquired from a Pyrococcus species. The general loss-only scenario is incompatible with a comparison of intein and extein phylogenies. If the phyletic pattern could be explained with one gain and subsequent losses, the datasets of the host extein phylogenetic tree and the intein tree should be compatible. However, the AU and SH tests, and the GARD analysis all confirm these trees are incompatible. The GARD algorithm identifies breakpoints three amino acids upstream of the extein intein junctures. This slight deviation from the true intein extein boundary likely reflects a limitation of the approach due to the insertion site and the last three amino acids of the intein being highly conserved. Consequently, little phylogenetic information is contained in the amino acids directly neighboring the intein/extein boundary. However, alternative or additional explanations are possible: the positions in the extein upstream of the intein participate in catalyzing the splicing reaction (Paulus 2000; Saleh and Perler 2006), thus it is not be surprising that they have a signature similar to the intein's splicing domain; during homing the sequence surrounding the target site also is copied from the invading template containing the intein (Chevalier and Stoddard 2001). The incongruence between the extein and intein trees in conjunction with the extein to intein divergence ratio data and the ancestral state reconstruction data all suggest that the inteins in the vma-1b position were horizontally acquired several times. The phylogenetic incongruence between the extein and intein tree provides strong support for the horizontal transfer of the T. litoralis intein from within the Pyrococcus spp.. The extein to intein divergence ratios suggest several additional transfers between P. horikoshii and P. furious; T. litorialis and P. horikoshii; T. litorialis and P. furious. The most likely scenario to explain these findings would be a within group transfer between the two Pyrococcus spp. followed by a transfer to T. litorialis. The phylogenetic incompatibility and the extein and intein substitution rate ratios taken together are strong support for a transfer from a Pyrococcus sp. to T. litorialis. This is the first report of the vma-1b intein found in a bacterial lineage. Although the region where M. australienses falls on the intein tree is not well resolved, the extein to intein ratios suggest several transfers of the intein between the Candidatus Nanosalinarium and Thetis sea lineages, the Methanothermus and Mahella lineages, the Methanothermus and Ferroplasma/Picrophilus clade, and the Mahella and Ferroplasma/Picrophilus clade. The phylogenetic distribution relative to its host gene and the ratio data of the vma-1b intein taken together suggest an interdomain transfer between M. australienses and the Archaea. However, we cannot conclude that the intein was directly transferred between specific lineages; it is possible that both lineages recently received the intein from a third, currently unsampled lineage.

!"+#"!"

Conclusions

Here we surveyed the distribution of the vma-1b intein and showed that this family of inteins is found in more diverse species than previously reported. These inteins were transferred between divergent organisms in several independent horizontal transfer events, including an HGT from within the Pyrococci to Thermococcus litorialis, a likely transfer to Candidatus Nanosalinarum and a transfer across the domain boundaries between the Archaea and Bacteria. While an intein may persist within a lineage for a long time without transfer between species (Barzel et al. 2011; Butler et al. 2006; Gogarten and Hilario 2006; Yahara et al. 2009), our data convincingly show that transfer of the intein between divergent species did occur and contributed to the observed modern day distribution of this intein.

Materials and Methods

Sequences. For the large archaeal type ATPase tree sequences were gathered from the NCBI's non- redundant database. Sequences for the smaller fifteen sequence datasets were gathered from either the NCBI non-redundant, whole genome shotgun, or metagenome databases. The MG-RAST database was also surveyed for additional inteins but did not reveal any. All databases were queried in February 2012. The A-ATPases sequence from the Thetis sea metagenome (Ferrer et al. 2012) was translated from contig 00086 (gi|3548555569) after correction of frame shifts. Phylogenetic Trees. For most datasets (except the ribosomal reference) sequences were aligned with SATe 2.03 (Liu et al. 2009) using the following options: MAFFT for the initial alignment, MUSCLE for the merger, RAxML with a gamma plus invariant sites LG substitution model (PROTGAMMAILGF) for tree estimation. Intein splicing domain and extein sequences were also aligned using muscle (Edgar 2004) and PRANK (Loytynoja and Goldman 2010). Maximum likelihood phylogenies calculated from these alignments (WAG Gamma + F + I) for the exteins were identical to the one given in Figure 2. For both the muscle and the PRANK alignments, the T. litoralis intein sequence grouped as sister to P. horikoshii within the clade formed by the Pyrococcus homologs. For both alignments the grouping of the T. litoralis intein with P. furiosus and P. horikoshii inside the Pyrococcus clade was supported by a bootstrap support value of 96%. In the intein maximum likelihood phylogeny calculated from the PRANK alignment, the Ferroplasma and Picrophilus inteins group with the Thermoplasma homologs, but without significant support (52%). The phylogenetic trees were reconstructed using PhyML v3.0, with the best parameters determined by ProtTest3 Maximum likelihood phylogenies, approximate Likelihood Ratio Test (aLRT) and bootstrap support values were determined using PhyML v3.0. under the WAG+G+F+I model. Posterior probabilities were calculated in MrBayes v3.1.2 (Huelsenbeck and Ronquist 2001; Ronquist and Huelsenbeck 2003) using the WAG+G+F+I substitution model (Whelan and Goldman 2001) and two runs. After 70,000 generations the standard deviation of split frequencies were 0.004317 and 0.008746 for the extein and intein datasets, respectively (small standard deviation of split frequencies indicate adequate convergence of the two runs, the MrBayes tutorial recommends smaller than 0.01 as stop criterion). After inspection of the likelihood trace, the first 700 and 450 generations were discarded as burnins (beginning phase of the tree search that hasn’t reach stationarity) for the extein and intein datasets, respectively.

!"+$"!"

Archaeal ribosomal phylogeny. We generated an archaeal ribosomal reference tree from a concatenated alignment of 62 ribosomal proteins (supplementary multiple sequence alignments) extracted from genomes downloaded from the NCBI ftp site. The ribosome tree included all archaeal taxa from the extein dataset except those that did not have genome representation, such as Thetis Sea contig from a metagenome project (Ferrer et al. 2012). This led to a total of 81 Archaea. The number of taxa in each alignment ranged from 19-81 (see Supplementary Table 1 for the complete taxon set). BLASTP (Altschul et al. 1997) searches with reference sets of bacterial and archaeal ribosomal proteins against each archaeal genome and an e-value cut-off of 1E-10 identified homologs. In most cases, each sequence from a reference set returned the same best BLAST hit from a genome; interpreted as strong evidence of homology. We also accepted instances where the majority of reference sequences returned the same best BLAST hit as evidence of homology (such as ratios of 80:1, where 80 references sequences returned the same best BLAST hit and only one differed). Muscle (Edgar 2004) aligned each protein set and Trimal v.1.2 (Capella-Gutierrez et al. 2009) with the “automated1” option trimmed ambiguous regions from all alignments, which were concatenated into a supermatrix with an in-house Python script. Phyml generated a maximum likelihood phylogeny with 100 bootstrap replicates under the same parameters as those used to generate the extein genealogy. Reduced taxa genealogies We generated an extein genealogy and corresponding ribosomal phylogeny exclusively for organisms that contained inteins involved in HGT to highlight the correspondence between gene and reference tree. Phyml constructed ML trees under the same parameters as those employed for the full archaeal trees with 100 bootstrap replicates. PhyloBayes v.3.3e (Lartillot et al. 2009)performed Bayesian analyses under the CAT mixture model (Lartillot and Philippe 2004) with global exchange rates estimated by WAG and two chains for each analysis. The bpcomp option estimated the convergence of the two chains from the maximum difference of the bipartition frequencies. A total of 72,787 and 14,650 cycles were run for the reduced extein and ribosomal trees, respectively. The maximum bipartition frequency difference for the extein genealogy was 0.02 and 0.06 for the ribosomal phylogeny. We employed the CAT model for its efficacy in reducing or eliminating the effects of long-branch attraction. Three taxa were susceptible to LBA artifacts in the ribosomal tree due to amino acid composition and incomplete ribosomal datasets from incomplete genomes – Micrarchaeum acidiphilium, Nanosalinarum sp., and Nanosalina sp. The bacterial sequence from Mahella australiensis (which served as an outgroup) attracted Nanosalinarum to the base of the Archaea in the reduced ribosomal phylogeny and even the CAT model failed to overcome this LBA artifact. Topology tests CONSEL v.0.2 performed both AU and SH tests to determine whether trees and their bootstrap replicates were significantly different from each other. Site-likelihoods were calculated in RAxML v.7.3.5 (Stamatakis et al. 2008). Breakpoint Analysis. The Genetic Algorithm for Recombination Detection (GARD) (Kosakovsky Pond et al. 2006) as implemented on the Datamonkey web server (Delport et al. 2010) was used to determine potential points of recombination in the fifteen vma-1 proteins that harbor inteins. Sequences were aligned using SATé 2.1 (Liu et al. 2012), and sequences for the homing endonucleases were deleted prior to the analysis. Substitution Rate Ratios. Pairwise maximum likelihood distances were calculated for each extein and each intein protein splicing domain alignment using TREE-PUZZLE 5.2 (Schmidt et al. 2002) using the WAG+F +I model for substitution and a Gamma distribution approximated through four discrete rate categories, such that the model was the same for all other phylogenic reconstructions. The extein and the !"+%"!" intein splicing domain were extracted from the SATe alignment (see above). The estimated alpha shape parameter for the intein splicing domains was 3.01 (S.E. 0.11), whereas the extein sequences showed a stronger ASRV with an estimated alpha of 1.37 (S.E. 0.04). To estimate the substitution rate ratio between extein and intein sequences under vertical inheritance, we used the distances estimated with TreePuzzle between Thermoplasma acidophilum DSM 122-1B2, Thermoplasma volcanium GSS1, and the Thermoplasmatales archaeon I-plasma (see table 1 and the spreadsheet in supplementary materials). The inteins in these Thermoplasmatales do not contain a homing endonuclease domain indicating they are no longer mobile and were likely inherited together with the extein (see figure 2). To obtain a phylogenetically independent estimate of the rate ratio, trees were calculated from the intein and extein distances within the Thermoplama using FITCH from the PHYLIP package (Felsenstein 2011), and rate ratios were calculated from the branch lengths. Ratios that were significantly larger than the within Thermoplasma ratios (mean ratio 0.30; SEM 0.13), which represent co-inheritance of intein and extein, suggest that the inteins are more similar to each other than under the assumption of co-inheritance with the exteins, indicating an intein transfer relative to the extein. Although direction of the intein transfer cannot be determined using these ratios recent transfers can be detected. Values greater than 0.70 were considered to be significantly greater than the Thermoplasma ratios (p ! 0.002). The high cut-off level was chosen to avoid false positives. False positive rates for the substitution ratio test. We performed two simulation studies to estimate the rate of false positives associated with the rate ratio cut-off used to identify putative transfer events. An intein tree, under the assumption of vertical inheritance only, was derived from the extein tree by dividing the branch lengths in the extein tree by 0.3145. This value was obtained from the ratio of the tree lengths within the Thermoplama group between the actual extein and intein trees. For the first simulation we used the aforementioned trees and simulated 10,000 sequence sets for the intein and extein tree using Evolver (Yang 2007). The sequences were simulated under the WAG model and alpha values were taken from the actual trees, which were 3.01 and 1.37 for the intein and extein, respectively. The substitution ratios were calculated. Extein distances less then 0.5 were excluded, and the number of ratios above 0.7 counted as false positives. We also estimated the false positive rates incorporating the uncertainty in determining the rate ratio. This was done by randomly sampling 1000 rate ratios from a folded normal distribution with mean 0.3145 and standard deviation of 0.1343 (the SEM for the determined rate ratio). The sampled ratios were used to generate new intein trees. The simulated intein and extein trees were evaluated as described above. Maximum Parsimony Ancestral State Reconstruction. A parsimony ancestral state reconstruction and a maximum likelihood ansectral state reconstruction were both performed to determine where among the vma-1 protein family the inteins were gained. The presence/absence patterns of the inteins were converted to a binary form and the maximum parsimony ancestral state reconstruction was calculated using the ordered model and the maximum likelihood ancestral state reconstruction was calculated under the MK1 model (Lewis 2001). Both reconstructions were implemented in Mesquite (Maddison and Maddison 2011).

!"+&"!"

Supplementary Materials fasta_files.zip: Multiple sequence alignments in fasta format: Content: sate_alignment_of_Intein_sequences.fasta sate_alignment_of_Extein_and_Intein_sequences_(withoutHE).fasta sate_alignment_of_alignment_of_extein_sequences.fasta Concatenated_alignment_of_archaeal_ribosomal_proteins.fasta

Interleaved_alignments.pdf: interleaved alignments with annotations (SEE BELOW) ratios.xls: Spreadsheet used for calculation of substitution ratios

Supplementary_Table_1.pdf: Ribosomal proteins in supermatrix and number of taxa per alignment. (SEE BELOW)

Supplementary_Figures.pdf (SEE BELOW)

Acknowledgements

This work was supported by the National Aeronautics and Space Administration Astrobiology, Exobiology and Evolutionary Biology (grant numbers NNX08AQ10G and NNX13AI03G) and the NSF Assembling the Tree of Life (ATOL) (DEB0830024) programs. We thank Matthew Fullmer, David Williams, Tim Harlow, Ofir Cohen, and an anonymous reviewer for providing insightful discussions on the manuscript.

!"+'"!"

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" Supplementary Table 1. Ribosomal proteins in supermatrix and number of taxa per alignment. Ribosomal protein # sequences in alignment L13e 19 S25e 20 S26e 21 S30e 21 S1P 35 L34e 41 L14e 43 L30e 66 L37e 71 L39e 74 S27Ae 74 L24e 77 L29 78 L31e 78 S27e 78 L44e 79 S17e 79 S24e 79 L10 80 L14 80 L15e 80 L18ae 80 L18e 80 L1 80 L21e 80 L22 80 L23 80 L2 80 L3 80 L40e 80 L4 80 L7Ae 80 S11 80 S13 80 S14 80 S15 80 S17 80 S19e 80 S19 80 S28e 80 S2 80 S3Ae 80 S3p 80 S4 80 S6e 80 S8e 80 S9 80 L15 81 L16P 81 L18 81 L19e 81 L24 81 L30p 81 L32e 81 L5 81 L6 81 S10 81 S12 81 S4e 81 S5 81 S7 81 S8 81 Supplementary Figures

Figure S1. Breakpoint Analysis of the VMA-1b Extein and Intein sequences.

Figure S2. Maximum Likelihood Phylogenetic Tree of the VMA-1 Protein Family Illustrating the Distribution of the Catalytic ATPase Subunit.

Figure S3. Maximum Likelihood Ancestral Character State Reconstruction of vma-1b Intein Presence and Absence.

Figure S4. Phylogenetic Trees of the Ribosomal Reference (right) and Corresponding Extein Sequences (left).

Figure S5. Reduced Archaeal Extein (left) and Ribosome Reference (right) Trees for Taxa Showing Evidence of Intein HGT.

Figure S6. Nonparametric Bootstrap Phylogenetic Analysis of Intein Sequences including the Homing Endonuclease Domain.

Figure S7. Conservation Profile of the Extein and Splicing Domain.

Figure S8. Site Conservation in VMA-1b Extein and Intein Sequences.

0.77 222Aciduliprofundum_boonei_T469 109Methanospirillum_hungatei_JF-1 0.97 229Thermococcus_sibiricus_MM_739 300Thermococcus_litoralis_DSM_5473] 1 0.93 803Pyrococcus_yayanosii 230Pyrococcus_furiosus_DSM_3638 996Pyrococcus_sp._NA2 Archaea 0.93 0.95 0.93 231Pyrococcus_horikoshii_OT3 0.68 232Pyrococcus_abyssi_GE5 233Thermococcus_onnurineus_NA1 1 235Thermococcus_kodakarensis_KOD1 1 0.97 234Thermococcus_gammatolerans_EJ3 1 108Coprothermobacter_proteolyticus_DSM_5265 1 125Dictyoglomus_turgidum_DSM_6724 124Dictyoglomus_thermophilum_H-6-12 1 130Aminobacterium_colombiense_DSM_12261 1 131Thermanaerovibrio_acidaminovorans_DSM_6589 1 168Thermoanaerobacter_pseudethanolicus_ATCC_33223 165Thermosediminibacter_oceani_DSM_16646 0.97 0.51 178Clostridium_novyi_NT 0.97 179Clostridium_tetani_E88 0.69 184Clostridium_botulinum_A_str._ATCC_3502 1 0.99 191Fusobacterium_nucleatum_subsp._nucleatum_ATCC_25586 0.55 190Finegoldia_magna_ATCC_29328 0.69 0.99 171Anaerococcus_prevotii_DSM_20548 0.79 189Leptotrichia_buccalis_DSM_1135 0.84 188Streptobacillus_moniliformis_DSM_12112 0.91 175Clostridium_perfringens_SM101 1 177Clostridium_botulinum_E3_str._Alaska_E43 0.89 172Clostridium_cellulovorans_743B Bacteria 132Enterococcus_faecalis_V583 0.80 1 1 143Streptococcus_sanguinis_SK36 0.85 153Streptococcus_pyogenes_M1_GAS 0.95 144Streptococcus_gallolyticus_UCN34 140Streptococcus_pneumoniae_TIGR4 1135Streptococcus_pneumoniae_CGSP14 0141Streptococcus_mitis_B6 0.96 164Clostridium_thermocellum_ATCC_27405 0.70 0.78 158Clostridium_phytofermentans_ISDg 0.08 159Clostridium_difficile_630 0.27 163Mahella_australiensis_50-1_BON 1 127Acholeplasma_laidlawii_PG-8A 0.92 128Acidaminococcus_fermentans_DSM_20731 129Eubacterium_limosum_KIST612 0.72 223Methanopyrus_kandleri_AV19 301_Thetis_Sea 0.97 237Methanocaldococcus_vulcanius_M7 0.80 1 236Methanocaldococcus_infernus_ME 1 241Methanococcus_aeolicus_Nankai-3 1 242Methanococcus_voltae_A3 0.96 0.98 246Methanococcus_maripaludis_C6 243Methanococcus_vannielii_SB 1 997Methanothermus_fervidus_DSM_2088 0.99 226Methanothermobacter_thermautotrophicus_str._Delta_H 0.92 224Methanosphaera_stadtmanae_DSM_3091 0.97 228Methanobrevibacter_smithii_ATCC_35061 227Methanobrevibacter_ruminantium_M1 1 121Picrophilus_torridus_DSM_9790 0.85 120Ferroplasma_acidarmanus_fer1 1 999Ferroplasma_acidarmanus 0.98 0.80 302Thermoplasmatales_archaeon I-plasma whole genome shotgun sequence 0.24 Acid_Mine/ 1 1 119Thermoplasma_volcanium_GSS1 1 998Thermoplasma_acidophilum_122-1B2 118Thermoplasma_acidophilum_DSM_1728 0.98 801Micrarchaeum 802Parvarchaeum 1 205Ferroglobus_placidus_DSM_10642 0.72 206Archaeoglobus_fulgidus_DSM_4304 207Archaeoglobus_profundus_DSM_5631 204Methanosaeta_thermophila_PT 0.92 0.99 211Methanohalobium_evestigatum_Z-7303 0.55 213Methanohalophilus_mahii_DSM_5219 0.84 212Methanococcoides_burtonii_DSM_6242 Archaea 1 1 208Methanosarcina_barkeri_str._Fusaro 0.98 0.99 210Methanosarcina_mazei_Go1 0.92 209Methanosarcina_acetivorans_C2A 0.98 218Methanospirillum_hungatei_JF-1 1 217Methanoplanus_petrolearius_DSM_11571 0.22 219Methanoculleus_marisnigri_JR1 0.84 0.92 221Candidatus_Methanosphaerula_palustris_E1-9c 0.93 0.81 216Methanocorpusculum_labreanum_Z 220Candidatus_Methanoregula_boonei_6A8 1 214Methanocella_paludicola_SANAE 215uncultured_methanogenic_archaeon_RC-I 999Nanosalinarum_sp._J07AB56 1 800Nanosalina 0.99 0.97 197Haloquadratum_walsbyi_DSM_16790 195Halorubrum_lacusprofundi_ATCC_49239 0.05 1 198Haloferax_volcanii_DS2 0.96 196Halorhabdus_utahensis_DSM_12940 0.99 200Halomicrobium_mukohataei_DSM_12286 0.57 0.00 199Haloarcula_marismortui_ATCC_43049 0.52 201Natronomonas_pharaonis_DSM_2160 0.95 0.97 202Haloterrigena_turkmenica_DSM_5511 203Natrialba_magadii_ATCC_43099 0.37 194Halalkalicoccus_jeotgali_B3 193Halobacterium_salinarum_R1 1 122Methanocella_paludicola_SANAE 0.65 123Methanospirillum_hungatei_JF-1 0.89 49Aminobacterium_colombiense_DSM_12261 0.96 48Geobacter_uraniireducens_Rf4 0.94 1 73Anaeromyxobacter_sp._Fw109-5 75Anaeromyxobacter_dehalogenans_2CP-1 1 110Thermus_thermophilus_HB27 0.99 114Deinococcus_radiodurans_R1 Bacteria 0.84 115Truepera_radiovictrix_DSM_17093 116Meiothermus_ruber_DSM_1279 92Nitrosopumilus_maritimus_SCM1 0.31 105Ignicoccus_hospitalis_KIN4 0.98 0.55 106Hyperthermus_butylicus_DSM_5456 104Acidilobus_saccharovorans_345-15 0.99 107Aeropyrum_pernix_K1 0.98 0.96 93Sulfolobus_acidocaldarius_DSM_639 1 94Sulfolobus_tokodaii_str._7 0.94 95Metallosphaera_sedula_DSM_5348 1 0.84 96Sulfolobus_solfataricus_P2 0.40 102Sulfolobus_islandicus_M.16.27 85Vulcanisaeta_distributa_DSM_14429 1 0.42 84Caldivirga_maquilingensis_IC-167 Archaea 1 79Pyrobaculum_islandicum_DSM_4184 0.93 82Pyrobaculum_aerophilum_str._IM2 80Thermoproteus_neutrophilus_V24Sta 1 78Candidatus_Korarchaeum_cryptofilum_OPF8 0.93 77Thermofilum_pendens_Hrk_5 1 87Ignisphaera_aggregans_DSM_17230 0.99 89Staphylothermus_marinus_F1 1 90Desulfurococcus_kamchatkensis_1221n 91Thermosphaera_aggregans_DSM_11486 3Brachyspira_murdochii_DSM_12563 1 1 5Bacteroides_fragilis_NCTC_9343 0.12 1 4Bacteroides_vulgatus_ATCC_8482 10Parabacteroides_distasonis_ATCC_8503 8Porphyromonas_gingivalis_ATCC_33277 1 1 11Halomonas_elongata_DSM_2581 1 0.25 13Cyanothece_sp._PCC_8801 12Legionella_longbeachae_NSW150 0.57 31Desulfarculus_baarsii_DSM_2075 0.87 29Candidatus_Protochlamydia_amoebophila_UWE25 1 0.75 30Waddlia_chondrophila_WSU_86-1044 1 26Chlamydophila_felis_Fe 0.30 16Chlamydophila_pneumoniae_CWL029 1 19Chlamydia_muridarum_Nigg 0.71 25Chlamydia_trachomatis_B 0.95 0.15 46Spirochaeta_thermophila_DSM_6192 0.90 45Spirochaeta_smaragdinae_DSM_11293 0.96 1 35Borrelia_burgdorferi_B31 38Borrelia_recurrentis_A1 1 42Treponema_pallidum_subsp._pallidum_str._Nichols 1 44Treponema_denticola_ATCC_35405 0.93 41Fibrobacter_succinogenes_subsp._succinogenes_S85 32Desulfohalobium_retbaense_DSM_5692 Bacteria 1 68Allochromatium_vinosum_DSM_180 0.98 1 70Nitrosococcus_halophilus_Nc4 69Sideroxydans_lithotrophicus_ES-1 62Halothermothrix_orenii_H_168 0.96 0.98 64Thermosediminibacter_oceani_DSM_16646 0.68 63Clostridium_sticklandii_DSM_519 0.80 66Eubacterium_limosum_KIST612 0.97 0.57 59Anaerococcus_prevotii_DSM_20548 0.99 65Finegoldia_magna_ATCC_29328 1 60Clostridium_phytofermentans_ISDg 58Acholeplasma_laidlawii_PG-8A 0.64 0 57Thermotoga_sp._RQ2 52Thermanaerovibrio_acidaminovorans_DSM_6589 0.93 47Stackebrandtia_nassauensis_DSM_44728 0.86 50Nautilia_profundicola_AmH 0.96 53Treponema_pallidum_subsp._pallidum_str._Nichols 55Spirochaeta_smaragdinae_DSM_11293 0.3

Figure S2. Maximum Likelihood Phylogenetic Tree of the VMA-1 Protein Family Illustrating the Distribution of the Catalytic ATPase Subunit. Support values on the nodes of the tree were determined by the approximate likelihood ratio test. Highlighted in brown are bacterial and in purple are archaeal representatives. 25586 ta H Delta 33223 CC m ATCC leatu 20731 3502 27405 SM 12261 2088 2160 CC SM 11571 SM 1728 DSM 16790 hanolicus AT CC um Z7303 SM 5511 NA1 DSM 3638 DSM SM 4304 ans SM 12940 ius D SK36 igat DSM sp nuc m subsp AV19 SM 17093 se D biense s D ans R1 pseudet DSM str rophicus str er thermautot gali B3 s 501 BON x D leatu er evest boonei T469 r dehalogenans 2CP1 J07AB56 ctri ca D rkmenica walsbyi vannielii SB A str AT str ulinum A onnurineus pharaonis KOD1 kodakarensis kandleri sanguinis s jeot m sp um bot s radiodur ium nuc undum ccu hermobact hermus fervidus radiovi co australiensi ccu s thermophilus HB27 myxobacte rrigena tu alinaru ali ococcus habdus utahensi ridium ridium thermocellum AT Mine r hanohalobium hanot hanopyrus hanot hanococcus ronomonas rept ahella anosalina naero hermu ruepera hermococcus m D idophilu hermoplasma ac hermoanaerobact hermococcus Streptobacillus moniliformis DSM 12112 Leptotrichia buccalis DSM 1135 obacter Fus 5 Pyrococcus abyssi GE OT3 Pyrococcus horikoshii Pyrococcus sp NA2 Pyrococcus furiosus Pyrococcus yayanosii DSM 5473 Thermococcus litoralis MM 739 Thermococcus sibiricus Met DSM 3091 Methanosphaera stadtmanae M1 Methanobrevibacter ruminantium ATCC 35061 Methanobrevibacter smithii Met H612 Dictyoglomus thermophilum DSM 6724 Dictyoglomus turgidum 5 Coprothermobacter proteolyticus DSM 526 Thermosediminibacter oceani DSM 16646 T Methanococcus aeolicus Nankai3 Methanococcus aeolicus Met C6 Methanococcus maripaludis A3 Methanococcus voltae vulcanius M7 Methanocaldococcus infernus ME Methanocaldococcus JF1 Methanospirillum hungatei Aciduliprof Clostridium novyi NT Clostridium tetani E88 Clost Geobacter uraniireducens Rf4 Anaeromyxobacter sp Fw1095 A Thetis Sea T 3 gammatolerans EJ Thermococcus T Enterococcus faecalis V583 Eubacterium limosum KIST612 Acidaminococcus ferment Acholeplasma laidlawii PG8A M Clostridium phytofermentans ISDg Clostridium difficile 630 Clost Halalk Haloarcula marismortui ATCC 43049 Halomicrobium mukohataei DSM 12286 Nat Natrialba magadii ATCC 43099 Halote Met Anaerococcus prevotii DSM 20548 Finegoldia magna ATCC 29328 Clostridium botulinum E3 str Alaska E43 Clostridium perfringens SM101 Clostridium cellulovorans 743B Streptococcus pneumoniae TIGR41 Streptococcus mitis B6 Streptococcus pneumoniae CGSP14 Streptococcus gallolyticus UCN34 Streptococcus pyogenes M1 GAS St N Nanos Methanospirillum hungatei JF1 Methanocella paludicola SANAE Meiothermus ruber DSM 1279 T Deinoco T Aminobacterium colom Ferroglobus placidus DSM 10642 Met Methanohalophilus mahii DSM 5219 Methanococcoides burtonii DSM 6242 Methanosarcina barkeri str Fusaro Methanosarcina acetivorans C2A Methanosarcina mazei Go1 Methanosaeta thermophila PT Candidatus Methanoregula boonei 6A8 Methanocorpusculum labreanum Z Candidatus Methanosphaerula palustris E19c Methanoculleus marisnigri JR1 Methanospirillum hungatei JF1 hanoplanus petrolear Met Aminobacterium colombiense DSM 12261 6589 Thermanaerovibrio acidaminovorans DSM Thermoplasmatales archaeon Acid Ferroplasma acidarmanus Ferroplasma acidarmanus fer1 Picrophilus torridus DSM 9790 T Thermoplasma acidophilum 1221B2 Thermoplasma volcanium GSS1 Parvarchaeum Micrarchaeum Archaeoglobus profundus DSM 5631 Archaeoglobus fulgidus D Methanocella paludicola SANAE uncultured methanogenic archaeon RCI Halorubrum lacusprofundi ATCC 49239 Haloquadrat Haloferax volcanii DS2 Halobacterium salinarum R1 Halo

Euryarchaeota Actinobacteria Firmicutes Deltaproteobacteria Dictyoglomi Deinococcus-Thermus

Figure S3. Maximum Likelihood Ancestral Character State Reconstruction of vma-1b Intein Presence and Absence. The depicted cladogram represents the part of the VMA-1 tree where inteins are present (see Supplemental Figure S2 for full tree). Presence and absences of the intein are represented at the tips of the tree; black circles correspond to presence of the intein and white circles correspond to absences of the intein. Partially filled circles represent the probability of an intein present at the node. This reconstruction was calculated under the MK1 model (Lewis 2001) as implemented in mesquite (Maddison and Maddison 2011).

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Figure S4. Phylogenetic Trees of the Ribosomal Reference (right) and Corresponding Extein Sequences (left). Maximum likelihood trees were generated by Phyml. Trees are rooted between Euryarchaeota and Crenarchaeota. Colored branches represent identical subtree topologies between the extein and ribosome trees. Gray branches indicate clades comprised of the same members between the two trees but different internal topologies. Bootstrap values < 50% not shown; 0.3, 0.3 amino acid substitutions per site.

Figure S5. Reduced Archaeal Extein (left) and Ribosome Reference (right) Trees for Taxa Showing Evidence of Intein HGT. Bootstrap support values for the maximum likelihood trees generated in Phyml and posterior probabilities for the Bayesian trees constructed in PhyloBayes are shown to the left and right of the forward slash, respectively. Trees are rooted with the bacterium, Mahella australiensis, which also shows evidence of intein HGT. While the intein genealogy displays incongruence with the extein tree - suggestive of HGT - the extein tree is congruent with the ribosome reference tree, which is not prone to HGT. The only topological difference stems from the incorrect placement of Candidatus Nanosalinarum sp. in the ribosome tree due to LBA from unusual amino acid composition and an incomplete ribosomal protein dataset. Note, with greater taxonomic sampling in the complete archaeal tree (see supplementary figure 3), Candidatus Nanosalinarum sp. groups with the Halobacteria, which is considered the proper placement for this taxon. Thermoplasma acidophilum 0.2 DSM 1728 100

Thermoplasma acidophilum 92 122-1B2

Thermoplasma volcanium GSS1

Thermoplasmatales archaeon I-plasma

Pyrococcus abyssi GE3 100

Pyrococcus sp. NA2

100

90 Pyrococcus furiosus DSM 3638

96 Pyrococcus horikoshii OT3

98 52 Thermococcus litoralis DSM 5473

Candidatus Nanosalinarum sp. J07AB56

100 Thetis Sea contig00086

Methanothermus fervidus DSM 2088

64 Mahella australiensis 50-1BON

74 Ferroplasma sp. Type II

97 Picrophilus torridus DSM 9790

Figure S6. Nonparametric Bootstrap Phylogenetic Analysis of Intein Sequences including the Homing Endonuclease Domain. The analysis was performed using phyml with the WAG model, and Gamma plus I model with estimated parameters for among site rate variation. See material and methods for details.

6

5

4

3

2

1

0 0 100 200 300 400 500 600 700 800 900 Figure S7. Conservation Profile of the Extein and Splicing Domain. The y-axis gives the average number of amino acid in a ten amino acid sliding window of the alignment and the x-axis is the sequence position in the alignment. In orange is the extein and in green is the intein splicing domains. A

Extein Intein Extein Histogram of average248 number418 of aa in a 3 aa window B

Extein Number of occurrences 50 100 150 200 0

12345678

C 40

30 Intein 20 Number of occurrences 10 0

12345678 Number of aa per site in a 3 aa window of the sequence alignment

Figure S8. Site Conservation in VMA-1b Extein and Intein Sequences. Panel A gives the conservation profile for the extein and intein splicing domain in a three amino acid sliding window. The bar gives the location of extein (purple) and intein (orange) sequences. Dotted lines indicate the breakpoints determined in the GARD analysis. Panel B and C give histograms for average conservation per site in three amino acid windows of extein (B) and intein (C) sequences respectively. Note that the three amino acids at the carboxy terminal end of the intein are completely conserved in this data set, and that this level of conservation occurs more frequently in the extein sequences. Interleaved Alignments with Annotations

Content: Alignment of Extein Sequences page 1-5 Alignment of Extein and Intein Sequences (without HE) page 6-7 page 8 Alignment of Intein Sequences Alignment: /Users/jpgogarten/Dropbox/supplementary material for upload/alignement_of_extein_sequences.fasta Seaview [blocks=50 fontsize=4 LETTER] on Fri Jul 19 09:36:36 2013 1

1 25Chlamydia_trachomatis_B/TZ1A828/OT/1591/1-591 M------VATSKQ------TTQGYVVEAYGNLLRVHVD---- GHVRQGEMAYV------SVDDTW------LKAEIIEVVGDEVKIQVF EETQGISRGALVTFSGHLLEAELGPGLLQGIFDGLQNRLEILADT----- 801Micrarchaeum/1586/1-586 MI------DMAGIIYRISGPVVIAQGL---- DDPNMYDVVRV------GETK------LVGEIIKLDGDKAIIQVY EDTSGLKPGEPVENTGTQLSVDLGPGLLGSIYDGIQRPLDKIKEMT---- 222Aciduliprofundum_boonei_T469/1582/1-582 M------GRIIRVAGPVVVADGM---- KGSEMYEVVKV------GEEG------LIGEIIGLYGDTATIQVY EETSGIKPGEPVERTGAPLSVMLGPGIISQIYDGIQRPLPEIKELT---- 127Acholeplasma_laidlawii_PG8A/1592/1-592 MKEHEKM------TNQGIITKVSGPLIVAKNM---- GSAQVYDVVRV------SDKR------LIGEVLELRGDLASIQVY EETAGIGPGEPVFLTNEPLSVELGPGLIGGIFDGIQRPLDSIYQIH---- 85Vulcanisaeta_distributa_DSM_14429/1603/1-603 MS------SDKGRIVYISGPVVKAE-L---- PGALLYELVFV------GELG------LFGEVVRIQGDMAFIQVY EETTGLKPGEPVVRTGEPLSAWLGPGILNRVYDGVQRPLPLIFDLT---- 210Methanosarcina_mazei_Go1/1578/1-578 M------EVKGEIYRVAGPVVTAIGL---- -DAKMYDLCKV------GNEG------LMGEVIQIVGDKTIIQVY EETGGVRPGEPCVTTGMSLAVELGPGLLSSIYDGVQRPLHVLLERT---- 8Porphyromonas_gingivalis_ATCC_33277/1584/1-584 M------ATKGVVKGIVSNLVTVEVD---- GPVSQNEICYI------DVNGTK------LMSEVIKVIGKNAYVQVF ESTRGMHVGDEAEFTGSMLEVTLGPGMLSKNYDGLQHDLDKMD------216Methanocorpusculum_labreanum_Z/1581/1-581 MEVN------SKPGILKRIAGPVVTAVDL---- -DAHMYDVVKV------GNEE------LMGEVIKIDGADTVIQVY ESTTGIRPGEPVINTGLSLAVELGPGLLTSIYDGIQRPLEVLIQKM---- 212Methanococcoides_burtonii_DSM_6242/1578/1-578 M------EVNGEIYRVAGPVVTVIGI---- -KPRMYDVVKV------GHEG------LMGEVIRIKGEQATVQVY EDTSGLKPGEPVMNTGLPLSVELGPGLLESIYDGIQRPLPVLQEKM---- 242Methanococcus_voltae_A3/1585/1-585 M------VVGKIIKIAGPVVVAEGM---- KGSQMYEVVKV------GNEG------LTGEIIQLNENEAIIQVY EETAGMQPGEPVEGTGAPLSVELGPGMLKAMYDGIQRPLNAIEDAT---- 132Enterococcus_faecalis_V583/1595/1-595 MS------VQIGKIVKVSGPLILAENM---- SDASIQDICHV------GDLG------VIGEIIEMRGDVASIQVY EETTGIGPGEPVISTGEPLSVELAPGLIAEMFDGIQRPLDTFQEVT---- 63Clostridium_sticklandii_DSM_519/1594/1-594 MM------EKTAITISVNGPVVGAKNM---- TGFKVREMVMV------GEKK------LLGEVISLDGDTGTIQVY EETEGLKSGENVYSTGNPLSLTLGPGMIGNVFDGIQRPLEAIEKIS---- 215uncultured_methanogenic_archaeon_RCI/1579/1-579 M------SQQGTIYRVAGPVVTAVGL---- -NARMYDVVKV------GNEG------LMGEVIEIDNDKAIIQVY EDTSGVRPGEPVENTGMPLSVELGPGLLTSIYDGIQRPLEVLKEKM---- 121Picrophilus_torridus_DSM_9790/1922/1-589 M------SGSIYSVSGPVVIAQDI---- ENAKMFDVVRV------GELG------LIGEIIRISGNKATIQVY EDTSGLRPGEKVYSTGKPLSVELGPGLLSSIYDGIQRPLDVIRAKT---- 209Methanosarcina_acetivorans_C2A/1578/1-578 M------EVKGEIYRVAGPVVTAIGL---- -DAKMYDLCKV------GNEG------LMGEVIQIVGGKTIIQVY EETGGVKPGEPCVTTGMSLAVELGPGLLSSIYDGVQRPLHVLLEKT---- 128Acidaminococcus_fermentans_DSM_20731/1590/1-590 M------SGKIVKVSGPLIVAEGM---- KDVQLHDMVRV------SDKR------LIGEVIELRDDKASIQVY EETAGLGPGEPVESTGSPMSVELAPGLIESIFDGIQRPLEKIYELA---- 5Bacteroides_fragilis_NCTC_9343/1585/1-585 M------ATKGTVSGVIANMVTLVVD---- GPVAQNEICYI------STGGDK------LMAEVIKVVGTHVYVQVF ESTRGLKVGAEAEFTGHMLEVTLGPGMLSKNYDGLQNDLDKMD------96Sulfolobus_solfataricus_P2/1592/1-592 M------DNGRIVRINGPLVVADNM---- RNAQMYEVVEV------GEPR------LIGEITRIEGDRAFIQVY EDTSGIKPNEPVYRTGAPLSIELGPGLIGKIFDGLQRPLDSIKELT---- 75Anaeromyxobacter_dehalogenans_2CP1/1579/1-579 M------SGTLMRMAGPTVVAEGL---- SGASLNEVVRV------GEER------LLGEIIRIEGDRATIQVY EETAGLALGEPVEASGEPLAVELGPGLLGSVFDGVQRPLSELAARE---- 235Thermococcus_kodakarensis_KOD1/1585/1-585 M------GRIIRVTGPLVVADGM---- KGAKMYEVVRV------GEIG------LIGEIIRLEGDKAVIQVY EETAGIRPGEPVEGTGSSLSVELGPGLLTAMYDGIQRPLEVLRQLS---- 92Nitrosopumilus_maritimus_SCM1/1592/1-592 M------AAQGRIVWVSGPAVRADGM---- SEAKMYETVTV------GDSK------LVGEVIRLTGDVAFIQVY ESTSGLKPGEPVIGTGNPLSVLLGPGIIGQLYDGIQRPLRALSEAS---- 190Finegoldia_magna_ATCC_29328/1587/1-587 ------MKEGKVLKVSGPLVVAEGM---- ENASVYDVVEV------SDDK------LIGEIIEMRGDKASIQVY EETTGIGPGDKVVSTGHPLSIELGPGILEQMFDGIQRPLKSLQEKA---- 999Nanosalinarum_sp._J07AB56/1584/1-584 MTQETI------ESEGEIYKITGPVVVAEDL---- -DCQMNDVVYV------GGEE------LLGEVIQIEGGQAYIQVY EETTGVSPGEPVKNTGEPLSVELGPGLLGSIYDGLQRPLPELEEQM---- 50Nautilia_profundicola_AmH/1571/1-571 M------LKITSINGPIVKAEYKN--- ETPLMYEFVYI------GKSR------LIGEIISINKNFITIQVY EDTTSLKLNEPVFLTGSLLSASLGPGLLGSVYDGIQRELFSMPER----- 82Pyrobaculum_aerophilum_str._IM2/1593/1-593 M------SGKIEYISGPVVKAE-L---- PGARLYELVFV------GEIK------LFGEVVRIQGEKAFIQVY EDTTGLKPGEPVERTGEPLSAWLGPTIIGKIYDGVQRPLRNIEEIS---- 68Allochromatium_vinosum_DSM_180/1598/1-598 MSET------NKIGVIRDINGPIVTIE-L---- PGARSGEQVKI------GELG------LYGEILSLNGERAVVQTY ESTDGVRPGEPVVGLGWPLSVELGPGLMGGIFDGVQRPLLKLALQS---- 89Staphylothermus_marinus_F1/1592/1-592 MSL------GITGKIYRVSGPLVIAENM---- RGSKVYEVVEV------GSDR------LIGEIIGVEGDKAIIQVY EDTSGLRVGDPVYGTGYPLAAELGPGLVGSIYDGIQRPLPLLQELV---- 184Clostridium_botulinum_A_str._ATCC_3502/1592/1-592 MN------LKTGRVLKISGPLVVAEGM---- EEANIYDVVKV------GEKR------LIGEIIEMREDRASIQVY EETAGLAPGDPVITTGEPLSVELGPGLIEAMFDGIQRPLNAIKAKA---- 205Ferroglobus_placidus_DSM_10642/1573/1-573 M------EVGEVYRVSGPLVVAEGL---- -KARMYDVCRV------GEER------LMGEVVGLVGNRVLIQVY EDTSGIKPGDKVENTGMPLSVELGPGLLKSIYDGVQRPLPALKEAS---- 87Ignisphaera_aggregans_DSM_17230/1591/1-591 M------EKTGRIYRVSGPLVVAEDI---- -KAMMYEVVYV------GEEG------LIGEVIAIQGDKTYIQVY EETTGLTVGEKVIGTGRLLSAELGPGLIGSIYDGLQRPEKVIGEVT---- 206Archaeoglobus_fulgidus_DSM_4304/1581/1-581 MEVKEA------GYVGEIYRISGPLVVAEGL---- -KARMYDLCKV------GEEG------LMGEVVGLVGQKVLIQVY EDTEGVKPGDKVENTGMPLSVELGPGLIRNIYDGVQRPLPVLKEVS---- 108Coprothermobacter_proteolyticus_DSM_5265/1589/1-589 M------HGQGYVVRVAGPLVTAKGL---- EKPMMYEVVRV------GELK------LFGEIIGIEKDLVYIQVY EDTNGLRPGETVEPTGQLLSVELGPGLTNNIFDGIQRPLERLREES---- 91Thermosphaera_aggregans_DSM_11486/1584/1-584 M------EKKGVIYRISGPLVIAENV---- KGVGVYEVVEV------GEER------LIGEVIGVEQDRAVIQVY EDTTGLKIGEPVYLTGQPLSAELGPGLITSIFDGIQRPLPVIQELA---- 246Methanococcus_maripaludis_C6/1586/1-586 M------VVGKIIKISGPVVVAEGM---- KGSQMFEVVKV------GNEG------LTGEIIQLTEDEAIIQVY EETAGIKPGEGVTGTGAPLSVELGPGMLKAMYDGIQRPLNEIENAT---- 135Streptococcus_pneumoniae_CGSP14/1599/1-599 MDLVR------RITLTQGKIIKVSGPLVIASGM---- QEANIQDICRV------GKLG------LIGEIIEMRRDQASIQVY EETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLAT---- 125Dictyoglomus_turgidum_DSM_6724/1589/1-589 M------EGKIVRVAGPLVVAKGL---- PNVKMYEMVRV------GELE------LFGEVIGLDNELVYIQVY EETQGIGPGEPVYALGEPLSVELGPGIVSQIYDGIQRPLNKLAEIS---- 11Halomonas_elongata_DSM_2581/1627/1-627 MDERIMTQDTVASMP------AATARVVAINEDVVTIELDDDAT GCLIKNEVVYICPPSSVDRPRTL------LKAEVLSVKGNEAEAQVY EDTRNVGVGDPVIQSGQQLTVELGPGLLGQVYDGLQNPLPRLLETG---- 159Clostridium_difficile_630/1592/1-592 ------MKTGTIVKVSGPLVVAEGM---- RDANMFDVVRV------SDKH------LIGEIIEMHGDKASIQVY EETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDIS---- 800Nanosalina/1586/1-586 MTQADLQQA------EADGEIYKVTGPVVVATGM---- -NPQMYDVVHV------GQEG------LMGEVIQIEGEKSYIQVY EETTGVKPGEPIENTGEPLSVDLGPGLLTSIYDGLQRPLPKLEQMT---- 996Pyrococcus_sp._NA2/1588/1-588 M------PVKGEIIRVTGPLVVAKGM---- KGAKMYEVVRV------GELG------LIGEIIRLEGDKAVIQVY EETAGVRPGEPVIGTGSSLSVELGPGLLTSIYDGIQRPLEVIREKT---- 219Methanoculleus_marisnigri_JR1/1584/1-584 MDKGKEN------RAQGVLKRISGPVVTAVGL---- -DAHMYDVVKV------GNEE------LMGEVIKIQGENIIIQVY EDTAGIRPGESVGNTGLSLAVELGPGLLTSIYDGIQRPLEVLVDKM---- 65Finegoldia_magna_ATCC_29328/1588/1-588 M------NNGKIFSINGPVIKGRNM---- TDFTAKEMVLI------GEKK------LIGEVISLEDDIAVIQVY EETEGLKKDDEIYHTNKPLSLKLGPGLLKNMFDGIERPLKEIRDKF---- 38Borrelia_recurrentis_A1/1576/1-576 M------EAKGKVVGVIGNLVTIEMV---- GTVSMNEIVFI------KTGGQS------LKAEIIRIRDGEVDAQVF EMTRGIAVGDDVEFTDKLLTVELGPGLLSQVYDGLQNPLPELATKC---- 32Desulfohalobium_retbaense_DSM_5692/1589/1-589 MTDPHATSTAQNDA------QATGRIVGVNGNLVVTEFD---- TPIFQNETALV------HCHDLR------LPAEIVRIRGNRAEMQIF EDTKDLRVGDTVSFSGELLSVELGPGLLGQVFDGLQNPLYDMAETY---- 163Mahella_australiensis_501_BON/1587/1-587 ------MSQGSIVKVSGPLVVAQGM---- GDANMYDVVRV------SGMG------LIGEIVEIHGDMAYIQVY EETSGLGPGEPVESTGQPLSVELGPGLIEAIYDGIQRPLNAIREQA---- 129Eubacterium_limosum_KIST612/1601/1-601 MIQPNEQSTM------KQVGTIVKVAGPLIVATGL---- ADVQMFDVVRV------SEKR------LIGEVIELRGDRASIQVY EETAGLGPGEPVYVTGEPLSVDLAPGLIESIFDGIQRPLTTIYNES---- 69Sideroxydans_lithotrophicus_ES1/1599/1-599 M------GKVVEVNGPLVTVD-L---- ADVPTGEQVRM------GSLD------LVGEVIARNGAHALVQMY EPTESLRPGEPVVALGHPLSVELGPGLLGNIFDGVQRPLLEHAQQH---- 214Methanocella_paludicola_SANAE/1579/1-579 M------SLVGEIFRVAGPVVTAVGL---- -QARMYDVAKV------GKEG------LMGEVIEIADDKTIIQVY EDTSGLRPGEPVENTGMPLSVELGPGLLTSIYDGIQRPLPILKSKM---- 70Nitrosococcus_halophilus_Nc4/1593/1-593 M------GKLLEVNGPLVRAR-L---- PQVPTGEQVKV------GKIG------LVGEVIGREGEEALIQVY EATESVRPGEAVEPLGYPLSVELGPGLLGQIFDGIQRPLDRILEVS---- 229Thermococcus_sibiricus_MM_739/1585/1-585 M------GKIVRVTGPLVVADEM---- RGSRMYEVVRV------GELG------LIGEIIRLEGDKAVIQVY EETAGIKPGEPVMGTGASLSVELGPGLLTSIYDGIQRPLEILRSQS---- 175Clostridium_perfringens_SM101/1591/1-591 ------MKTGKIIKVSGPLVVAEGM---- DEANVYDVVKV------GEKG------LIGEIIEMRGDKASIQVY EETSGIGPGDPVITTGEPLSVELGPGLIESMFDGIQRPLDAFMKAA---- 179Clostridium_tetani_E88/1592/1-592 MD------LKTGRVVKVSGPLVVAEGM---- EEANLFDVVRV------GDER------LIGEIIEMREDKASIQVY EETSGLGPGAPVVTTGAPLSVELGPGLIEAMFDGIQRPLDAIEAKA---- 107Aeropyrum_pernix_K1/1597/1-597 M------AVKGSIVRISGPLVVAEGM---- SGAQMYEMVYV------GEDR------LIGEITRIRGDRAFIQVY ESTSGLKPGEPVVGTGAPLSVELGPGLLGTIYDGVQRPLPIIAEKVAEVD 105Ignicoccus_hospitalis_KIN4/I/1596/1-596 M------AVKGKVLRVRGPVVIAEDM---- QGVQMYEVVEV------GKDG------LVGEVTRITGDKAVIQVY EDTTGITPGEPVVGTGSPFSVELGPGLLSHIFDGILRPLESIHEVA---- 301Thetis_Sea/1-587/1-751 M------GRIIRVAGPVVTADGM---- RGTEMHEITRV------GDEE------LIGEVIELEEDKATIQVY EETSGLQPGEKVDRTDEPLSVELGPGMIGTVFDGIQRPLPKIREES---- 31Desulfarculus_baarsii_DSM_2075/1581/1-581 M------NATGKVLAAYGNLVTVQFD---- SNVRQNEVAYI------VTADGR------MKSEVIRVRGDQCYAQVF EDTRGIKVGDAVEFTGDLLVVELGPGILQQIYDGLQNPLPKLSEAT---- 158Clostridium_phytofermentans_ISDg/1589/1-589 ------MSKGVIKKVAGPLVIAEGM---- RNANMFDVVRV------SSQR------LIGEIIEMHGDEASIQVY EETSGLGPGEPVESTGNPLCVELGPGLIGSIFDGIQRPLNEIMERT---- 193Halobacterium_salinarum_R1/1585/1-585 MSQAE----AI------TDTGEIESVSGPVVTATGL---- -DAQMNDVVYV------GDEG------LMGEVIEIEGDVTTIQVY EETSGIGPGQPVDNTGEPLTVDLGPGMLDSIYDGVQRPLDVLEDEM---- 46Spirochaeta_thermophila_DSM_6192/1591/1-591 M------ITGRVVGVNGNMVRVEVD---- GNVRMNEVAYV------CVEDKK------LKSEVIRIRGKHAELQVF EITRGIGVGDLVEFTGELLSVKLGPGLLAQIYDGLQNPLPELARKC---- 196Halorhabdus_utahensis_DSM_12940/1587/1-587 MSQATT-S-DV------REDGVIESVSGPVVTATGL---- -QARMNDVVRV------GREG------LMGEIIEIEGDLTTIQVY EETSGVAPGGPVESTGAPLSVDLGPGLLDNIYDGVQRPLEGLEEKM---- 12Legionella_longbeachae_NSW150/1603/1-603 MKDKLPEHP------SHDARVIAIQEGIVQIRQQ--GP KPIIKNEIIYICPLQQQGTVPQR------IMAEILHVKGDTAIAQVF EETSGIAIGDRVEQSGQQLSVTLGPGLLGMIYDGLQNPLINFASKY---- 236Methanocaldococcus_infernus_ME/1589/1-589 M------AGKIVKIAGPVVVAEGM---- KGSQMYEVVKV------GEEK------LTGEIIQLHGDRAVIQVY EETTGVKPGEPVIGTGSPLSVELGPGMLRAMYDGIQRPLTAIEEKT---- 300Thermococcus_litoralis_DSM_5473]/1585/1-585 M------GRIIRVTGPLVVADEM---- RGSRMYEVVRV------GELG------LIGEIIRLEGDKAVIQVY EETAGVKPGEPVVGTGASLSVELGPGLLTSIYDGIQRPLEILREKS---- 231Pyrococcus_horikoshii_OT3/1964/1-588 M------VAKGRIIRVTGPLVVADGM---- KGAKMYEVVRV------GELG------LIGEIIRLEGDKAVIQVY EETAGVRPGEPVVGTGASLSVELGPGLLTSIYDGIQRPLEVIREKT---- 73Anaeromyxobacter_sp._Fw1095/1578/1-578 M------TARLVRIAGPTVVADGL---- AGAALNEVVWV------GEER------LLGEVIRLEGDRATIQVY EETAGLALGEPAEPSGEPLAVELGPGLLGSVFDGVQRPLDALAAQQ---- 194Halalkalicoccus_jeotgali_B3/1587/1-587 MSQATQTDIDV------VSEGRIESVSGPVVSATDL---- -DARMNDVVYV------GEEG------LMGEVIEIEGNVTTIQVY EETSGISPGGPVKNTGDPLSVDLGPGMLDTIYDGVQRPLDVLESRM---- 202Haloterrigena_turkmenica_DSM_5511/1587/1-587 MSQAEDIE-SV------DEDGVIESVSGPVVTATDL---- -DARMNDVVYV------GDEG------LMGEVIEIEGNLTTIQVY EETSGVGPGEPVQNTGEPLSVDLGPGMLDSIYDGVQRPLDVLEGKM---- 997Methanothermus_fervidus_DSM_2088/1585/1-585 M------TGEKRIIRVSGPVVVAEGM---- KGSQMYEMVRV------GEEK------LIGEIIELEGDKATIQVY EDTTGLKPGEKVETTGGPLSVELGPGVLGEIFDGIQRPLDKIKAMT---- 802Parvarchaeum/1583/1-583 M------EGKIIGISGSVVTVSGL---- EDPKMNNIVFI------GGNE------LVGEIVKIEGENAIIQVY EDTSGLRIGEKAVDSNEPLSVELGPGLIGSIFDGIQRPLDKILDKE---- 208Methanosarcina_barkeri_str._Fusaro/1578/1-578 M------EVKGEIYRVSGPVVTVTGL---- -QAKMYDLVKV------GDEG------LMGEVIQILGPKTIIQVY EETAGIKPGEPCYSTGSSLSVELGPGLLSSIYDGVQRPLHVLLERT---- Ferroplasma_sp._Type_II L------GKIIRVSGPVVVAEDI---- ENQKMFDVVRV------GELN------LVGEIIKLNGNRATIQVY EDTSGIKPGEKVETTGKPLSVELGPGLLKSIYDGIQRPLDIIRESS---- 84Caldivirga_maquilingensis_IC167/1595/1-595 MMSS------QGTGRILVVNGPVIKAE-L---- PGAKLYELVFV------GELG------LFGEVVRVQGENAFIQVY EDTTGIRPGEPVVRTGEMLSAWLGPGIIGQVYDGVQRPLKIIFDQT---- 197Haloquadratum_walsbyi_DSM_16790/1585/1-585 MSQ-TE-Q-AV------REDGVIRSVSGPVVTARGL---- -DARMNDVVYV------GDEG------LMGEVIEIEDNVTTVQVY EETSGIGPDEPVRNTGEPLSVDLGPGLLDTIYDGVQRPLDILEDKM---- 232Pyrococcus_abyssi_GE5/11017/1-588 M------VAKGRIIRVTGPLVVADGM---- KGAKMYEVVRV------GELG------LIGEIIRLEGDKAVIQVY EETAGVRPGEPVIGTGSSLSVELGPGLLTSIYDGIQRPLEVIREKT---- 200Halomicrobium_mukohataei_DSM_12286/1586/1-586 MSQATE-S-DV------REDGVIDSVSGPVVTATGL---- -DARMNDVVYV------GSEG------LMGEVIEIEGDITTIQVY EETSDVSPGEPVEGTGSPLSVDLGPGMLDAIYDGVQRPLDVLEEKM---- 143Streptococcus_sanguinis_SK36/1623/1-623 MKSKLLLSLLFDFHGEARKISQEWRKIVSQGKIIKVSGPLVLASGM---- QEANIQDICRV------GDLG------LIGEIIEMRRDQASIQVY EETSGLGPGEPVITTGSPLSVELGPGLISQMFDGIQRPLERFQTIT---- 30Waddlia_chondrophila_WSU_861044/1596/1-596 M-----NQESNASS------DVKGKVVKAFGNLLEVKFD---- GSIRQGEVAMV------QVGSLE------LKAEVIEIIGDIAKIQVF EDTKGIQLNTPVRFTGDLLEAELGPGLLSSIFDGLQNPLVEVAEEA---- 45Spirochaeta_smaragdinae_DSM_11293/1588/1-588 MD------RSSGKVIGVNGNMVSVLVD---- GTVSLNEVGYI------VLDEKR------LKSEVIRIRGDRAEMQVF EMTGGVGVGDSVEFTGELLAVELGPGLLTQIYDGLQNPLPELAQQC---- 131Thermanaerovibrio_acidaminovorans_DSM_6589/1569/1-569 ------MYDVVRV------GNMG------LVGEIIELKGDTASIQVY EETSGLMPGEPVVSTGEPLSVELAPGLIEQFYDGVQRPLNSIESVA---- 42Treponema_pallidum_subsp._pallidum_str._Nichols/1589/1-589 MT------QTKGIVSAVNGNMVSVTFE---- GVVSLNEVGYV------HVGNAR------LKAEIIRVRGREAQLQVF EITRGVSVGDRVEFTGDLLSVELGPGLLGQVYDGLQNPLPLLAEKV---- 77Thermofilum_pendens_Hrk_5/1601/1-601 MSG------QRKGYIAKVSGPVVIAKGI---- SDIKMGEVVYV------GNEG------LLGEVVRVSQDSFAVQVY EDTSGLRPKEPVTATGKLLVAELGPGLMGRVFDGVQRPLKSIEELV---- 110Thermus_thermophilus_HB27/1578/1-578 M------IQGVIQKIAGPAVIAKGM---- LGARMYDICKV------GEEG------LVGEIIRLDGDTAFVQVY EDTSGLKVGEPVVSTGLPLAVELGPGMLNGIYDGIQRPLERIREKT---- 203Natrialba_magadii_ATCC_43099/1587/1-587 MSQAEDIE-SV------DQDGVIESVSGPVVTATDL---- -DARMNDVVYV------GDEG------LMGEVIEIEGNLTTIQVY EETSGVGPGEPVQNTGEPLSVDLGPGVLDAIYDGVQRPLDVLEEKM---- 93Sulfolobus_acidocaldarius_DSM_639/1592/1-592 M------AGEGRVVRVNGPLVVADGM---- RNAQMFEVVEV------GELR------LVGEITRIEGDRAYIQVY EATDGIKPGEKAYRTGSLLSVELGPGLMGGIFDGLQRPLDRIAESV---- 35Borrelia_burgdorferi_B31/1575/1-575 M------NTKGKVVGVNGNLVTIEVE---- GSVSMNEVLFV------KTAGRN------LKAEVIRIRGNEVDAQVF ELTKGISVGDLVEFTDKLLTVELGPGLLTQVYDGLQNPLPELAIQC---- 55Spirochaeta_smaragdinae_DSM_11293/1592/1-592 MSE------RIIGTVGRVNGPVIEAEGI---- SDAIMMELVHV------GNKR------LVGEVIKLHGNSATLQVY EDTTGIAPGDPIYGSGLPLSVELGPGLIGTIYDGIQRPLERIREVC---- 29Candidatus_Protochlamydia_amoebophila_UWE25/1593/1-593 M----KTLETRKEK------KAQGKVLKAFGNLLQVTFE---- GNIRQGEVAMV------HVDNLQ------LKSEVIEILGNQAKIQVF EDTKGVRLGSLVSFTGDLLEAELGPGLLSSIFDGLQNPLVDVADQA---- 198Haloferax_volcanii_DS2/1586/1-586 MSQATQ-D-SV------REDGVIASVSGPVVTARGL---- -DARMNDVVYV------GDEG------LMGEVIEIEGDLTTIQVY EETSGVGPGEPVESTGEPLTVDLGPGMMDAIYDGVQRPLDVLESKM---- 302Thermoplasmatales_archaeon/1-575 M------GKILRVAGPVVIGEDV---- EGAKMYDVVRV------GEMG------LVGEIIRMEGDKATIQVY EDTSGIKPGDKVESTLRPLSVLLAPGVLKSIYDGIQRPLDVIRAQT---- 62Halothermothrix_orenii_H_168/1590/1-590 M------DKKGEIVFVNGPVVKADKM---- NGFIMNELVYV------GKER------LIGEIIELEGDLATIQVY EETTEMQPGEPVFSTGSPLSVELGPGIIGNIFDGIQRPLPVIAEKT---- 115Truepera_radiovictrix_DSM_17093/1579/1-579 M------AIEGTIQRISGPAVIARGM---- MGARMFDIVRV------GNEK------LVGEIIRLDGDTAFVQVY EETGGLKVGEPVVSTGLPLAVELGPGMLNGIFDGIQRPLDKINEAS---- 16Chlamydophila_pneumoniae_CWL029/1591/1-591 M------VTVSEQ------TAQGHVIEAYGNLLRVRFD---- GYVRQGEVAYV------NVDNTW------LKAEVIEVADQEVKVQVF EDTQGACRGALVTFSGHLLEAELGPGLLQGIFDGLQNRLEVLAED----- 213Methanohalophilus_mahii_DSM_5219/1576/1-576 M------NGEIYRVAGPVVTVTGI---- -KPKMYDVVKV------GHEG------LMGEVIRIEGEKATVQVY EDTSGIKPGEPVENTGMPLSVELGPGLLESIYDGIQRPLEVLQQKM---- 78Candidatus_Korarchaeum_cryptofilum_OPF8/1595/1-595 MLEQTY------VGKGVIVHVKGPVVIAEGM---- RGSKIREVVYV------GEER------LIGEIVRIEEDRAVIQVY EPTGGVKAGEPVERTGEPLSAELGPGLLGQVLDGLGRPLDIIAEKA---- 19Chlamydia_muridarum_Nigg/1591/1-591 M------VATSKQ------TTQGYVVEAYGNLLRVHFD---- GHVRQGEVAYV------SVDDTW------LKAEIIEVVGDEVKVQVF EETQGISRGALVTFSGHLLEAELGPGLLQGIFDGLQNRLEVLADT----- 52Thermanaerovibrio_acidaminovorans_DSM_6589/1594/1-594 MSE------LKMGFVTMVNGPVIRGSSM---- GSFGMREMVHI------GSLG------LLGEVIRLEDDEALIQVY DDTQGLKVGEPIRGTGRSLSITLGPGLIGGFFDGIGRPLERLMESQ---- 223Methanopyrus_kandleri_AV19/1592/1-592 MSN------SVKGEIVKIAGPVVEAVGC---- EGAKMYEVFRV------GDEG------LIGEVINIESDRATIQVY EETTGLQPGEPVKGTGELLSVELGPGLLTQIFDGIQRPLPEIRKEV---- 189Leptotrichia_buccalis_DSM_1135/1597/1-597 MKGENS------LKTGKIIKVSGPLVVAEGM---- ENANVYDVVRV------SEKK------LIGEIIEMRGDQASIQVY EETAGIGPGEEVFTTGEPLSVELGPGLIEAMFDGIQRPLKEYQEIA---- 191Fusobacterium_nucleatum_subsp._nucleatum_ATCC_25586/1382/1-584 ------MKEGRIIKVSGPLVVAEGM---- EEANVYDVVEV------SENK------LIGEIIEMRGDKASIQVY EETTGIGPGDVVVTTGSPLSIELGPGMLEQMFDGIQRPLLKIQEAV---- 4Bacteroides_vulgatus_ATCC_8482/1584/1-584 M------ATKGTVSGVIANMVTLTVD---- GPVAQNEICYI------LTGGDR------LMAEVIKVVGSNVYVQVF ESTRGLKVGAEAEFTGHMLEVTLGPGMLSKNYDGLQNDLDKMD------95Metallosphaera_sedula_DSM_5348/1591/1-591 M------AQGKVARVNGPLVVAEGM---- KDAQMFEVVEV------GEPR------LVGEITRIEGDRAYIQVY EDTSGIRPGEPVFGSGAPLSVELGPGLLGQLFDGLLRPLGEIKEIT---- 44Treponema_denticola_ATCC_35405/1589/1-589 MT------KTKGKVVGINGNMISVSFE---- GLVTLNEVGYV------EVGSKK------LKSEVIRIRGEVAQLQVF EITKGIKVGDIVEFTGDLLSVELGPGLLGQVYDGLQNPLPELAEQA---- 90Desulfurococcus_kamchatkensis_1221n/1584/1-584 MS------MHKGIIHRVSGPLVVAENV---- RGVSIYEVIEV------GEER------LIGEAIGVEGDKAIIQVY EDTTGLKVGDPVYPTGQPLSAELGPGLIKSIFDGIQRPLPVIEALS---- 57Thermotoga_sp._RQ2/1586/1-586 M------RVLEINGPVVRVENT---- EGLFMHEVIYV------GKMR------LTGEVIALDEKTAIVQVY EETSMLEPGELVERTGKMLSVALGPGLLGNIYDGIQRPLKVLLESS---- 803Pyrococcus_yayanosii/1587/1-587 M------PAKGRIIRVTGPLVVADDM---- KGAKMYEVVKV------GELG------LIGEIIRLEGDKAVIQVY EETAGIRPGEPVEGTGTSLSVELGPGLLTSIYDGIQRPLEALRELS---- 228Methanobrevibacter_smithii_ATCC_35061/1584/1-584 M------IIEGKIIKIAGPVIIADGM---- RGAQMYEMVRV------GEQK------LIGEIIELEGDTATIQVY EETAGIQPGEVVESTGGPLSVELGPGVMGSIFDGIQRPLELIREES---- 13Cyanothece_sp._PCC_8801/1607/1-607 MTET------FNPARVVAVQEDLVTIKMADTNP KPIIKNEVIYICPQRKSNGRQEK------LKAEILRVRGQEADAQVY ESTRGIGIGDIVEQTGELLSVTLGPGLLTQVYDGLQNPLAEVAADY---- 168Thermoanaerobacter_pseudethanolicus_ATCC_33223/1590/1-590 ------MSQGIITKVSGPLVVAEGL---- PEAKMFDVVKV------GNQG------LIGEIIEIRGERVSIQVY EETSGLGPGDPVVSTGEPLSVELGPGMLEGIFDGIQRPLDVIEKKV---- 26Chlamydophila_felis_Fe/C56/1588/1-588 M------VVTSGQ------TTQGYVVEAYGNLLRVRFD---- GHVRQGEVAYV------SVDDTW------LKSEVIEVVGQEVKIQVF EDTQDVCRGALVTFSGHLLEAELGPGLLQGIFDGLQNRLQVLAES----- 80Thermoproteus_neutrophilus_V24Sta/1594/1-594 M------SGRIEYIAGPVVKAD-L---- PGAKLYELVFV------GEIK------LFGEVVRVQGDKAFIQVY EDTTGLRPGEPVVRSGEPLSAWLGPTIIGKIYDGVQRPLKNIEEIS---- 114Deinococcus_radiodurans_R1/1582/1-582 MTQ------QKQGVVQSIAGPAVIAKGM---- YGAKMYDIVRV------GQER------LVGEIIRLDGDTAFVQVY EDTAGLTVGEPVETTGLPLSVELGPGMLNGIYDGIQRPLDKIREAS---- 122Methanocella_paludicola_SANAE/1579/1-579 M------LEGMISRISGPVIMARRM---- RGSKMYDVVKV------GDDE------LRGEVIRLEGDGAVIQLY EDSTGLKIGEKVVNTGAPLSVELGPGLISSIYDGIQRPLPALYENS---- 217Methanoplanus_petrolearius_DSM_11571/1582/1-582 MEVKG------KSKGILKRISGPVVTAVDL---- -DAHMYDVVKV------GDEQ------LMGEVIKIDGDNVIIQVY EATDGIRPGEPVENTGMSLAVELGPGLLTSIYDGIQRPLEVLMEKM---- 998Thermoplasma_acidophilum_1221B2/1590/1-590 M------GKIIRISGPVVVAEDV---- EDAKMYDVVKV------GEMG------LIGEIIKIEGNRSTIQVY EDTAGIRPDEKVENTRRPLSVELGPGILKSIYDGIQRPLDVIKITS---- 204Methanosaeta_thermophila_PT/1576/1-576 M------EVGRVKRVAGPVVQAVGL---- -KASMYDLVLV------GEEG------LMSEVIGISGDKHIIQVY EDTSGIKPGEPVKETGGPLVAQLGPGILTQIYDGVQRPLPLLAEKS---- 218Methanospirillum_hungatei_JF1/1582/1-582 MEVK------RTKGVLKRIAGPVVTAVNL---- -DAHMYDVVKV------GDEQ------LMGEVIKIKGEDIIIQVY EDTSGIKPGEPVENTGLSLSVELGPGLLTSIYDGIQRPLEVLVEKM---- 221Candidatus_Methanosphaerula_palustris_E19c/1588/1-588 MEVKAKEGKI------EGKGVLKRIAGPVVTAVDL---- -DAHMYDVVKV------GNEE------LMGEVIKIEGNNTIIQVY ENTSGIKPGEPVSNTGLSLAVELGPGLLTSIYDGIQRPLEVLMDKM---- 171Anaerococcus_prevotii_DSM_20548/1585/1-585 ------MNKGKITKVSGPLIEASGL---- SDANIYDVVEV------SKDK------LIGEIIEMRGDVASIQVY EETTGIGPGDEVVSTGHPLSVELGPGMLEKMYDGIQRPLEKLQDLA---- 120Ferroplasma_acidarmanus_fer1/1589/1-589 M------ENKGSIYSISGPVVIATDL---- -DGKMFDVVRV------GEMG------LVGEVIKIVGDKFTIQVY EDTSGLKPGEPVYSTGKPLSVELGPGLLKSIYDGIQRPLDIIQSET---- 119Thermoplasma_volcanium_GSS1/1776/1-590 M------GKIVRISGPVVVAEDI---- ENAKMYDVVKV------GEMG------LIGEIIRIEGNRSTIQVY EDTAGIRPDEKVENTMRPLSVELGPGLLKSIYDGIQRPLDVIKETS---- 3Brachyspira_murdochii_DSM_12563/1587/1-587 M------TKGNVTAIISNLISIEVD---- GPVSQNEICYV------SCGNAK------LMAEVIKISGTSASAQVF ESTRGVKLGDAVEFTGSMLEIELGPGLLGKNFDGLQNDLDKLQ------59Anaerococcus_prevotii_DSM_20548/1582/1-582 M------NPKIKTINGPVVIATDA---- KILTVREMVSV------GKLK------LIGEVISLEGDLATIQVY EDTSGLKVNEDIIPTGRPLSVRLGPGMLGNMFDGIQRPLKNIMDKN---- 164Clostridium_thermocellum_ATCC_27405/1589/1-589 ------MSQGTIVKVSGPLVIAEGM---- RDANMFDVVRV------SEHR------LIGEIIEMHGDRASIQVY EETAGLGPGEPVVSTGAPLSVELGPGLIENIFDGIQRPLVKMREMV---- 237Methanocaldococcus_vulcanius_M7/1587/1-587 M------PVVGKIIKIAGPVVVAEGM---- KGAQMYEVVKV------GEEK------LTGEIIQLHDDKAVIQVY EETSGIKPGEPVVGTGAPLSVELGPGMLRAMYDGIQRPLTAIEEKT---- 124Dictyoglomus_thermophilum_H612/1589/1-589 M------EGKIVRVAGPLVVAKGL---- PNVKMYEMVRV------GNLE------LFGEVIGLDNDLVYIQVY EETQGIGPGEPVYALGEPLSVELGPGIISQIYDGIQRPLNRLAEVS---- 153Streptococcus_pyogenes_M1_GAS/1591/1-591 ------MNQGKIITVSGPLVVASGM---- QEANIQDICRV------GHLG------LVGEIIEMRRDQASIQVY EETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKAT---- 41Fibrobacter_succinogenes_subsp._succinogenes_S85/1585/1-585 M------ASIGKITGVNGNLIRVKFE---- SAVSQNEVAYA------KLISKNEAGKTEIIPLKSEVIRIRGDYAELQVF EDTTGLKAGDEVEFTGELLSVELGPGLLTQVFDGLQNPLPKLADEC---- 220Candidatus_Methanoregula_boonei_6A8/1588/1-588 MEVKANQAKE------TKKGVLKRIAGPVVTAVNL---- -DAHMYDVVRV------GNEA------LMGEVIKIQGDNVIIQVY EDTTGIKPGEPVSNTGLSLAVELGPGLLTSIYDGIQRPLEVLVNKM---- 79Pyrobaculum_islandicum_DSM_4184/1593/1-593 M------SGKIEYISGPVVKAD-L---- PGARLYELVFV------GEIK------LFGEVVRVQGDKAFIQVY EDTTGLKPGEPVVRTGEPLSAWLGPTIMGKIYDGVQRPLKNIEEIS---- 177Clostridium_botulinum_E3_str._Alaska_E43/1592/1-592 ------MKTGKIIKVSGPLVVAEGM---- DEANIYDVCKV------GEKG------LIGEIIEMRGDKASIQVY EETSGIGPGDPVVTTGEPLSVELGPGLIESMFDGIQRPLDAFMEAA---- 48Geobacter_uraniireducens_Rf4/1524/1-524 M------TVDL---- KGLKLYDMVRV------GEAM------LIGEVVRLEQERAVVQVY EDTRGLGVHEPAKGLDTPLTARLGPGLLSGMFDGLQRPMERLFRQC---- 211Methanohalobium_evestigatum_Z7303/1578/1-578 M------EVKGEIYRVSGPVVIATGI---- -RPKMYDVVKV------GHEG------LMGEVIKIEGDKSTIQVY EDTSGIKPGEPVENTGLPLSVELGPGLLESIYDGIQRPLQVLQDKM---- 230Pyrococcus_furiosus_DSM_3638/11013/1-588 M------PAKGRIIRVTGPLVIADGM---- KGAKMYEVVRV------GELG------LIGEIIRLEGDKAVIQVY EETAGLKPGEPVEGTGSSLSVELGPGLLTSIYDGIQRPLEVLREKS---- 47Stackebrandtia_nassauensis_DSM_44728/1575/1-575 MN------QTFGRVRRVCGPLVDVDLS---- APVAMNETVWL------GTEG------IPGEVVAVRDDIATVQAY EDTGCLAPKAVVRTGGSPLSAPLGPGLLGGVFDGLLRPLSDAPD------199Haloarcula_marismortui_ATCC_43049/1586/1-586 MSQATD-T-DV------REDGIIESVSGPVVTARDL---- -DARMNDVVYV------GSEG------LMGEVIEIEGNITTIQVY EETSGVSPGEPVEGTGSPLSVDLGPGMLDAIYDGVQRPLDVLEEKM---- 227Methanobrevibacter_ruminantium_M1/1584/1-584 M------INEGNIIKIAGPVIVADGM---- RGTQMYEVVRV------GEAK------LIGEIIELEGDTATIQVY EETAGVKPGEKVESTGGPLSVELGPGVMGSIFDGIQRPLEVIKGIS---- 10Parabacteroides_distasonis_ATCC_8503/1585/1-585 M------ATKGIVKGIVSNLVTVEVD---- GPVSQNEICYI------SVGGVK------LMSEVIKVIGKNAFVQVF ESTRGMRVGDEAEFQGHMLEVTLGPGMLSRNYDGLQNDLDKME------195Halorubrum_lacusprofundi_ATCC_49239/1591/1-591 MSKAESTD-TT------AENGVIQSVSGPVVSARDL---- -DARMNDVVYV------GDEG------LMGEVIEIEGDITTVQVY EETSGVAPGEPVENTGEPLSVDLGPGMLDSIYDGVQRPLDVLEGKM---- 64Thermosediminibacter_oceani_DSM_16646/1587/1-587 M------ETGRIIYINGPVVRASGM---- KGFTMREMVLV------GEKK------LIGEVISLDKEVATIQVY EETSGLRAGEPVTGTGKPLSVKLGPGILGNIFDGIERPLSHIYKEE---- 144Streptococcus_gallolyticus_UCN34/1591/1-591 ------MSQGKIIKVSGPLVVASGM---- QEANIQDICRV------GDLG------LIGEIIEMRRDQASIQVY EETSGIGPGEPVVTTGNPLSVELGPGLISQMFDGIQRPLDRFKTAT---- 165Thermosediminibacter_oceani_DSM_16646/1590/1-590 ------MSQGVIAKVSGPLVVATGL---- PEAKMFDVVKV------GTQG------LIGEIIEIRNDKVSIQVY EETSGLGPGDPVVSTGEPLSVELGPGMIEGIFDGIQRPLDVIEKKV---- 172Clostridium_cellulovorans_743B/1591/1-591 MT------LKVGRIEKVSGPLVVAEGM---- DEANIYDVCKV------GDKG------LIGEIIEMRGDRASIQVY EETSGIGPGDPVVTTGEPLSVELGPGLLEAIFDGIQRPLDKFLQVS---- 201Natronomonas_pharaonis_DSM_2160/1585/1-585 MSQATAS--EI------TDEGVIESVSGPVVTAVDL---- -DARMNDVVYV------GEEG------LMGEVIEIEGNLTTIQVY EETSDVAPGEPVENTGEPLSVDLGPGMMDSIYDGVQRPLDVLEEKM---- 178Clostridium_novyi_NT/1591/1-591 ------MKTGRVIKVSGPLVIAEGM---- EEANIYDLVKV------GEKR------LIGEIIEMRGDKASIQVY EETTGLGPGAPVETTGEPLSVELGPGLIESMFDGIQRPLEAIAKKA---- 141Streptococcus_mitis_B6/1591/1-591 ------MTQGKIIKVSGPLVIASGM---- QEANIQDICRV------GKLG------LIGEIIEMRRDQASIQVY EETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLAT---- 109Methanospirillum_hungatei_JF1/1588/1-588 MG------EKNGVIIRVTGPVVEAEGM---- AGSRMYESVRV------GNDG------LIGEIIILEGDRATIQVY EETIGLTPGEPVVRTYLPLSVELGPGLIGTMYDGIQRPLEEILKNT---- 49Aminobacterium_colombiense_DSM_12261/1588/1-588 MGV------ASSGNITGISGPVIGAVTY---- TPIKMFEVAYI------GHSK------LLGEVIRIKGDRVDIQVY EDTSGLTKGEPVIFTGELLAVDLGPGILGSVFDGIGRPLEILGKD----- 118Thermoplasma_acidophilum_DSM_1728/1764/1-590 M------GKIIRISGPVVVAEDV---- EDAKMYDVVKV------GEMG------LIGEIIKIEGNRSTIQVY EDTAGIRPDEKVENTRRPLSVELGPGILKSIYDGIQRPLDVIKITS---- 94Sulfolobus_tokodaii_str._7/1595/1-595 MSNM------VSEGRVVRVNGPLVIADGM---- REAQMFEVVYV------SDLK------LVGEITRIEGDRAFIQVY ESTDGVKPGDKVYRSGAPLSVELGPGLIGKIYDGLQRPLDSIAKVS---- 102Sulfolobus_islandicus_M.16.27/1592/1-592 M------NNGRIVRINGPLVVADNM---- KNAQMYEVVEV------GEPR------LIGEITRIEGDRAFIQVY EDTSGIKPNEPVYRTGAPLSIELGPGLIGKIFDGLQRPLDSIKELT---- 53Treponema_pallidum_subsp._pallidum_str._Nichols/1605/1-605 MIKDD------VVTGRVVRVSGPIVYAEGL---- SACSVYDVVDV------GEAS------LIGEIIRLDESKAVVQVY EDDTGMRVGEKVTSLRRPLSVRLGPGLIGTIYDGIQRPLERLFQED---- 106Hyperthermus_butylicus_DSM_5456/1601/1-601 M------PVKGRIIRVAGPLVVAEGM---- EGIQMYEMVEV------GEER------LIGEVNRVVGDKAYIQVY ESTTGLKPGEPVYGTGSPLSVELGPGLIGRIYDGIQRPLDVIREVT---- 104Acidilobus_saccharovorans_34515/1601/1-601 M------VVRGRIYRISGPLVIAEGM---- TGAQMFEVVRV------GEEG------LIGEVTRIRGDMAYIQVY ESTSGLRPGEPVEGTGAPLSVDLGPGLIGSIFDGVQRPLPSIVATIARTN 58Acholeplasma_laidlawii_PG8A/1583/1-583 M------FMKNTIKVINGPVVKLGST---- DEFKMLEMVYV------GPKR------LIGEVISISDTETVIQVY ETTQGLKVGDLVYPTGELVSVMLGPGLMGNIFDGVLRPLQKIKDSD---- 130Aminobacterium_colombiense_DSM_12261/1598/1-598 MATEK------VIRGTIEKISGPLVVAKGM---- YGASMYDVVRV------GNIG------LVGEIIELKGDLASIQAY EETSGLMPGEPVVSTGEPLSVELGPGIIEQFYDGVQRPLNLIEDAA---- 243Methanococcus_vannielii_SB/1586/1-586 M------VVGKIIKISGPVVVAEGM---- KGSQMYEVVKV------GNEG------LTGEIIQLTENEAIIQVY EETAGIKPGEGVTGTGAPLSVELGPGMLKAMYDGIQRPLNAIEDAT---- 207Archaeoglobus_profundus_DSM_5631/1580/1-580 MEVRTV------GSVGEIYRISGPLVVAEGL---- -KAKMYDVCKV------GEEG------LMGEVVGIRGDKVLIQVY EDTSGVRPGEKVVNTGMPLSVELGPGMLTTIYDGVQRPLPVLKEVS---- 999Ferroplasma_acidarmanus/1589/1-589 M------KDKGTIYSISGPVVIATDL---- -DGKMFDVVRV------GNMG------LVGEVIKIVGNKFTIQVY EDTSGLKPGEPVRSTGKPLSVELGPGLLKSIYDGIQRPLDIIQSET---- 116Meiothermus_ruber_DSM_1279/1578/1-578 M------GTIKKIAGPAVIAENL---- QGAKMYDIVRV------GHEK------LVGEIIRLDGNTCFIQVY EDTNGLKVGEPVVTTGLPLALELGPGLLNGIFDGILRPLDKIQAVS---- 123Methanospirillum_hungatei_JF1/1584/1-584 M------ITGTIARISGPVITARNM---- TGSRMYDVVWV------GRAA------LPGEIIRLEGDEAVIQVY EDTTGLMIHEPVENTGVPLSVELGPGLLASIYDGVQRPLPALYAKS---- 226Methanothermobacter_thermautotrophicus_str._Delta_H/1584/1-584 M------TQEGRIIKIAGPVIIAEGM---- RGSQMYEMVKV------GEDK------LIGEIIELEGDTATIQVY EETAGIKPGETVERTGGPLSVELGPGILGSIFDGIQRPLENIKALT---- 241Methanococcus_aeolicus_Nankai3/1586/1-586 M------VSGKIVKIAGPVVVAEGM---- KGAQMYEVVKV------GNEG------LTGEIIQLESDKAVIQVY EETAGIVPGEPVEGTGAPLSAELGPGMLKAMYDGIQRPLTAIEDAT---- 234Thermococcus_gammatolerans_EJ3/1585/1-585 M------GRIVRVTGPLVVADGM---- KGAKMYEVVRV------GEMG------LIGEIIRLEGDRAVIQVY EETAGIKPGEPVIGTGSSLSVELGPGLLTSMYDGIQRPLEKLRELS---- 224Methanosphaera_stadtmanae_DSM_3091/1585/1-585 M------ITGNIIKIAGPVIIGDGM---- RGTQIHEMVRV------GDIG------LIGEIIELEGDTATVQVY EETAGIKPGEKIESTGGPLSVELGPGILKSIYDGIQRPLDEIKSVS---- 233Thermococcus_onnurineus_NA1/1585/1-585 M------GRIIRVTGPLVVADDM---- KGAKMYEVVRV------GEMG------LIGEIIRLEGDKAVIQVY EETAGIRPGEPVEGTGASLSVELGPGLLTAMYDGIQRPLEVLRELS---- 66Eubacterium_limosum_KIST612/1595/1-595 MSNEVKN------EVKGVISGINGPVVTGTNM---- TAFQMREMVMV------GKKK------LIGEVIVLDGDIGTIQVY EETEGLMPGEEILSTGMPLSVKLGPGMLKNMFDGIQRPLEKIQEIS---- 188Streptobacillus_moniliformis_DSM_12112/1588/1-588 M------QGRIVKVSGPLVVAENM---- QHANVFDMVKV------GNDK------LIGEIIEMRGDRASIQVY EETTGIGTNEPVETTGMPLSVELGPGLLENMFDGIQRPLDKIKQRV---- 140Streptococcus_pneumoniae_TIGR4/1591/1-591 ------MTQGKIIKVSGPLVIASGM---- QEANIQDICRV------GKLG------LIGEIIEMRRDQASIQVY EETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLAT---- 60Clostridium_phytofermentans_ISDg/1588/1-588 M------SETAKITGINGPVVYVKGK---- QQFRVAEMVLV------GKQN------LVGEVISLQKGMTTIQVF EETTGLRPGDEVTSTGSAISVTLAPGIISNIFDGIQRPLSEVAKQS---- 151 2 25Chlamydia_trachomatis_B/TZ1A828/OT/1591/1-591 ---SL-FLRRGE---YVN-AICRETVWAYTQK--AS------VGSVLSR GDVLGTVKEGR-FDHKIMVPFSC-----FEEVTITWVISSGNYTVDTVVA KGRTSTGEELEFTMVQKWPIKQAFL------EGEKVPSHEIMDVGLRVL 801Micrarchaeum/1586/1-586 ---GD-FIARGI---NVP-PLDLKKKWHFVPK--LK------AGDEANG GTVIGELDETSLIRHRVMVPPKL-----SGK--IKSI-KEGDYTVDEAVA VLSTESGD-VEIKLMQKWPVRIPRP------VKDKLPPEIPLITGQRVI 222Aciduliprofundum_boonei_T469/1582/1-582 ---GD-FIKRGA---TAP-PLNPNKKWHFVPR--AK------VGDYVRG GDILGTVQETLIVEHRIMVPPEK-----EGK--IEWIAEEGDYTIEEPIA KIDS-----AEIKMYQRWPVRRQRP------FKSKLDPVELLVTGQRVL 127Acholeplasma_laidlawii_PG8A/1592/1-592 ---GQ-GIPLGV---DIP-KLNREKLWQFIPQ--VE------VGDIVEG GDIIGIVHESESVVHKVMVPPNL-----SGT--VKSI-KSGEFTVVDTVC ELDHDGDI-LPLKMMQSWPVRIKRP------YNKKIAPTQPMVTGQRVI 85Vulcanisaeta_distributa_DSM_14429/1603/1-603 ---KRPFVARGINYEKAP-PLDFKKKWKWIPK--VK------VGDEVEV GDILGVVPETPLVEHRVLYPPIY---RVGGV--VKYVAPEGEYTIDDVIA EVETKEGT-VKVKMWHKWPVRRPRP------FKEKLPPIEPLITGIRTI 210Methanosarcina_mazei_Go1/1578/1-578 ---GG-FIGRGV---TAD-GLDHKKLWEFKPV--VK------KGDRVIG GDVIGVVQETVNIEHKIMVRPDI-----SGT--VADI-KSGSFTVVDTIC TLTDG----TELQMMQRWPVRKPRP------VKRKLTPEKPLVTGQRIL 8Porphyromonas_gingivalis_ATCC_33277/1584/1-584 ---GI-FLKRGD---YTP-ALDDDKLWDFKPL--AN------VNDNVIA GSWLGEVTENF-QPHKIMVPFVF-----EGNYKVKSLAKAGSYKVNDVIA VVTDQDGKDHNVTMVQKWPVKRAIT------CYREKPRPFKLLETGIRII 216Methanocorpusculum_labreanum_Z/1581/1-581 ---GN-FIARGV---TAP-GLDHEKKWTFKPV--VH------VGDNVVP GQIVGEVQETRSIVHKIMVPPLA----KGGK--VTKI-NAGDFTVDEIVI ELDSG----ESFPMMQKWPVRVPRP------YVEKHSPSIPLLTGQRIL 212Methanococcoides_burtonii_DSM_6242/1578/1-578 ---GN-FIQRGV---TAN-GLDRERVWEFKPT--VS------KGDEVKG GNILGLVQETKNIEHKIMVPPSI-----SGT--IKEI-KAGSFKVDETIC VLTDG----TEISMMQKWPVRGPRP------VAKKLMPTKPLITGQRIL 242Methanococcus_voltae_A3/1585/1-585 ---DSIYIPRGV---NVP-SLSRTAKWSFKPT--AK------VGDKVLG GDIIGTVEETASIVHKIMVPVNI-----NGT--IKEI-KEGEFTVEETVA VVETANGD-KDVIMMQKWPVRQPRP------NKEKLPPVIPLITGQRVE 132Enterococcus_faecalis_V583/1595/1-595 --HSN-FLGRGV---KID-ALDREKKWTFEPT--VA------VGEEVSA GDIVGVVQETPIIQHKIMVPFGV-----SGT--IAEI-KAGDFAIDETVY SVETAKGT-ESFSMMQKWPVRRGRP------ILEKLSPKVPMVTGQRVI 63Clostridium_sticklandii_DSM_519/1594/1-594 ---GG-FIAEGI---GLL-SLDKEKLWDVTIL--VS------EDDYVES GEIYATVQETSLIVHKLLIPPGV-----SGN--LKDVKPSGQYTIDEVVA TVSCSDRM-YPLKLHQVWPVREARP------VKERMAIDRLLITGQRVL 215uncultured_methanogenic_archaeon_RCI/1579/1-579 ---GN-FITRGV---SAP-GLSRTKKWKFVPV--VK------AGDKVKG GIVIGTVQETKTIVHKIMVPPNV----GETT--IKDI-KEGEFTVEDVIG HLENG----TELKLMHKWPVRVPRP------YVEKLRPDIPLITGQRVL 121Picrophilus_torridus_DSM_9790/1922/1-589 ---GD-FIAKGV---NIP-PLNEEKLWDFKPL--VN------EGQQVKS NFIIGEVDETEIIKNKIMVPYGV-----EGT--VKSI-KSGKFKVSDTVA IIETKNGD-YEIKLKQIWPVREARR------VFHKFPPEIPLITGQRVI 209Methanosarcina_acetivorans_C2A/1578/1-578 ---GG-FIQRGV---TAD-GLDHEKLWEFKPV--AK------KGDFVKG GEVLGVVQETVNLEHKVMMPPDK-----SGT--VADI-KSGNFTVLETVC TLTDG----TELQMMHRWPVRRPRP------VKRKLTPEKPLVTGQRIL 128Acidaminococcus_fermentans_DSM_20731/1590/1-590 ---GD-RISRGV---EVP-KLNREKKWQFVPV--AK------PGDQVSG GDVIGTVQETPAVLHKIMVPPGK-----SGT--VEWV-YEGEATILDPVY KLKDSKGNVQEYPMLQQWPVRVARP------YKTKLPPEEPMVTGQRVI 5Bacteroides_fragilis_NCTC_9343/1585/1-585 ---GV-FLKRGQ---YTY-PLDKGSVWHFVPL--VS------VGDKVEA SAWLGQVDENF-QPLKIMVPFTQ-----KGVCTVKSIVPEGDYKIEDVVA VLVDEEGNTVEVNMIQKWPVKRAMT------NYKEKPRPFKLLETGVRVI 96Sulfolobus_solfataricus_P2/1592/1-592 --KSP-FIARGI---KVP-SIDRKTKWHFVPK--VK------KGDKVEG GSIIGIVNETPLVEHRILVPPYV-----HGT--LKEVVAEGDYTVEDPIA IVDMNGDE-VPVKLMQKWPVRIPRP------FKEKLEPTEPLLTGTRVL 75Anaeromyxobacter_dehalogenans_2CP1/1579/1-579 ---GD-FLGRGA---SLP-ALDRTRAWEFEPA--VA------PGDRVEG GARLGVARAPGAPDHPVVVPPGV-----TGR--VAEV-RGGARRVDEPAV LLEGG----ATLALLERWPVRRPRP------ARRRLPPDVPFLTGQRVL 235Thermococcus_kodakarensis_KOD1/1585/1-585 ---GD-FIARGL---TAP-ALPRDKKWHFTPK--VK------VGDKVVG GDVLGVVPETSIIEHKILVPPWV-----EGE--IVEIAEEGDYTVEEVIA KVKKPDGTIEELKMYHRWPVRVKRP------YKQKLPPEVPLITGQRTI 92Nitrosopumilus_maritimus_SCM1/1592/1-592 ---GS-FIGRGI---TTT-PVDMAKKYHFVPS--VS------NGDEVAA GNVIGVVQETDLIEHSIMVPPDH----KGGK--ISNLVSEGDYDLETVLA TTEGEGET-VELKMYHRWPVRKPRP------YKNRYDPTVPLLTGQRVI 190Finegoldia_magna_ATCC_29328/1587/1-587 ---GD-FLLRGV---SAP-SLNREKKWEFKPV--KS------VGDEVEP GDIIGEVQETEVIVHKIMVPANV-----SGK--IKSI-ESGEFTVEQTVC VVEDKSND-IEINMIQRWPVRDGRP------YKEKEDPIKPLITGQRVI 999Nanosalinarum_sp._J07AB56/1584/1-584 ---GS-FIQRGE---DAP-GINPEEEYSFEPA--VE------EGDHVEP GDVIGTVQ-VSYGEHKVLLPPHS----DGGE--VEEV-REGNYTVTDHVA ELDTG----EKVSMRQEVPIRDQRP------AEEQLPPEVPLITGQRVF 50Nautilia_profundicola_AmH/1571/1-571 ------IQKGI---KSK-ALNEEKKYFFKAL--KN------VGDEVKK GEIIGEVDENG-IKHRILS--DF-----NGI--LNEI-ENKTCSIKDTVA VING-----NKQNMIKKRPLRIPGS------FKKRLSPKIPLITGQRVI 82Pyrobaculum_aerophilum_str._IM2/1593/1-593 ---KNPFIARGIGYDKAP-PLDLKAEFDFKPA--VK------PGEEVYP GDVLGSVKETEPMTHYILYPPLP--EHAPGV--VEWI-ADGKYKVDDVIA RVKTKRGV-VEVKMWHKWPVRRPRP------FKEKLPPVEPLITGVRTV 68Allochromatium_vinosum_DSM_180/1598/1-598 ---GD-YIRRGL---SVP-ALDRDKRWAFEPNAELE------PGAKVGP GTVLGTVQETLTIEHRILVPPGI-----EGE--LEEIAPAGEYDIESTIA RVRDHKGATHRLSLYQRWPVRKPRP------YRRRDDGISPLITGQRVI 89Staphylothermus_marinus_F1/1592/1-592 ---GF-FVKRGV---KAK-PLPRNKKWHFKPL--VK------QGEKVGP DDVIGYVEETPVIKHYIMIPPDT-----HGV--VEEIVGEGEYTIVDPIA RING-----KEIVMLQKWPVRKPRP------YREKLEHREPVLTGQRVI 184Clostridium_botulinum_A_str._ATCC_3502/1592/1-592 ---GD-FITKGV---EVH-SLDRDKKWHFTPV--KK------VGDTVEA GDVIGIVQETSIVEHKIMVPYGI-----KGT--IETI-EEGDFTVVDTVA KVKDKDKV-SDLMMMQKWPVRRGRP------YGRKLNPAQPMITGQRVI 205Ferroglobus_placidus_DSM_10642/1573/1-573 ---GD-FIGRGI---DAP-ALDRKKQWEFNPV--VK------KGDKVSG GDILGTVQETELIEHKILVPPNV-----EGV--ITEI-YEGKFTVEETVA VLDNG----VELKLYHKWPVRQPRP------YREKLPPQIPLITGQRIL 87Ignisphaera_aggregans_DSM_17230/1591/1-591 ---KSIFITRGV---KVA-ALDRSRKWLFERDKNIS------IGDKVGP GDIIGYVRETPIVIHKIMIPPGI-----SGT--LKEIVDDGDYAVEDTIA VVESGGNK-IEIKMYQQWPIRIPRP------YKAKLDPIEPLITGLRVI 206Archaeoglobus_fulgidus_DSM_4304/1581/1-581 ---GD-FIGRGI---EAP-GLDRKAKWEFKPL--VK------KGEKVKP GEIIGTVQETEVVEQKILVPPNV----KEGV--IAEI-YEGSFTVEDTIA VLEDG----TELKLYHKWPVRIPRP------YVEKLPPVVPLITGQRIL 108Coprothermobacter_proteolyticus_DSM_5265/1589/1-589 ---GE-FLARGF---SVN-ALDRQRKWHFVPL--AE------AGVEVEG GSFLGEVIESSTVVHKIMVPPNV-----KGT--LQWIAGEGEYTVEECIA KVELPNGTLQELQLMQRWPVRIPRP------VKEKVFTDVPMLTGQRIL 91Thermosphaera_aggregans_DSM_11486/1584/1-584 ---GI-FVRKGV---KTS-PLPRNKKWDFKPL--VK------PGAKVGP GDFIGVVKETELISHYIMVPLET-----RGI--VEWVAGEGSYTIEEPVA KING-----VEVKMYHKWPVRKPRP------FTEKLDSREPLVTGLRVI 246Methanococcus_maripaludis_C6/1586/1-586 ---DSIYIPRGV---SVP-SISREIKWDFEPI--AA------VGDEVIT GDVIGTVQETASIVHKIMIPFGV-----SGK--IKEI-KAGSFTVEETIA VVETAEGE-KEIMMMQKWPVRKPRP------SKGKQAPVIPLITGQRVE 135Streptococcus_pneumoniae_CGSP14/1599/1-599 --HND-FLVRGV---EVP-SLDRDIKWHFDST--IA------IGQKVST GDILGTVKETEVVNHKIMVPYGV-----SGE--VVSI-ASGDFTIDEVVY EIKKLDGSFYKGTLMQKWPVRKARP------VSKRLIPEEPLITGQRVI 125Dictyoglomus_turgidum_DSM_6724/1589/1-589 ---GD-FLSRGL---SFP-ALDREKKWNFEAR--VK------SGDYVEG GDILGVVQETSALEHRILVPPYL-----KGK--LLEI-KSGSYTVDEVIG ILEDENGNRHELKLMHKWPVRYQRP------VKEKLPPQIPLITGQRVL 11Halomonas_elongata_DSM_2581/1627/1-627 ---GT-FLQRGL---EVR-ALDDRHEWSFEAR--VR------SGDEVMP GDTLGVVQEGR-FSHRIFVPFAL-----QGTFSVAWI-QAGSFTIDTVVA RLTDEAGNEHPITMTQRWPVRHPLSQELVSLGRAERRYPEAPLTTTLRLI 159Clostridium_difficile_630/1592/1-592 ---GT-NITKGV---EVS-SLDRTTKWEFKPK--KS------VGDKVVA GDIIGGVQETAVVECKIMVPYGV-----KGT--IKEI-YSGEFTVEDTVC VITDEKGNDIPVTMMQKWPVRKERP------YREKKTPDSLLVTGQRVI 800Nanosalina/1586/1-586 ---GE-FISRGE---DAP-GIDLEEEYNFQPT--KQ------EGDQVEQ GDVLGEV-EVEFGTHKVLVPPSA----EEGE--IEEI-KEGSYTVEDTVV ELENG----AEISMRQEWPIREPRP------TEKDLKPEVPLKTGQRVL 996Pyrococcus_sp._NA2/1588/1-588 ---GD-FITRGV---TAP-ALPRDKKWHFIPK--VK------VGDKVVG GDIIGEVPETSIIVHKIMVPPGI-----EGE--IVEIAEEGDYTIEEVIA KVKTPSGEIKELKMYQRWPVRVKRP------YKEKLPPEVPLITGQRVI 219Methanoculleus_marisnigri_JR1/1584/1-584 ---GN-FIERGV---SAP-GLSHEKKWEFVPT--VK------KGDEVKA GDILGTVQETN-IVHKVMVPPKA----KGGK--IKKI-SGGSFTVDETVC VLEDG----TEIAMLQRWPVRVPRP------VTQKLNPDIPLITGQRIL 65Finegoldia_magna_ATCC_29328/1588/1-588 ---ST-FIPEGI---GLI-SIDEEKLWDVTIK--VK------NGDTVTQ GQIIAEVPETEAIVHKIMIPVGV-----NGT--VKNVLENGKYNIETTIM QVEDDFGNLTDIKLYQDWPIRIPRP------SKERLDVDRLLETGQRIL 38Borrelia_recurrentis_A1/1576/1-576 ---GF-FLERGL---YLS-ALDRNKKWSFNAT--AK------VGDFIVA GDFLGFVIEGT-INHKIMVPFDR-----RDSYRIIEIVGDGDYTVDDRIA VIEDDAGGKHIITMSFHWPVKVPVT------SYKKRLIPNETMVTQTRII 32Desulfohalobium_retbaense_DSM_5692/1589/1-589 ---GT-FLQRGA---SRP-ALDRTQHWEFTPE--VA------EGEEVKA GSWLGSVPEGI-FGHKLMVPFHL-----RGTYTVTRISPAGTYDLNTPLA TLTGPKGESIEVGLYQEWPVKIPIR------AYADRLRPTKPLSTKIRLI 163Mahella_australiensis_501_BON/1587/1-587 ---GD-RIARGV---RAP-SLDRDKKWHFTPV--IE------KGAHVTA GDIIGVVQETPIVEHRIMVPYGI-----EGT--VEEI-YEGDFTVEDTVA RVAVKGGI-KEITMMQHWPVRRGRP------YKRKLPPDRPLITGQRVI 129Eubacterium_limosum_KIST612/1601/1-601 ---GD-RISRGI---DVP-KLDREKKWPFVPA--CQ------KGDVLKP GDVIGTVQETPAVLQKIMMPPKL-----EGT--VDWV-FDGEANIVEPIA RIKKKDGEIVEVPMLRSWPVRRGRP------YTEKIAPDEPMVTGQRVI 69Sideroxydans_lithotrophicus_ES1/1599/1-599 ---GD-FIPRGV---HIP-SLNRSKLWHFEPQLGLK------IGSHVEG GTILGTVQETQTILHRILVPPGK-----DGE--LTELAGAGDITVDAVVA KVKDAKGNISELQLFHRWPVRIPRP------YRNRDNAVEPLLTGQRIL 214Methanocella_paludicola_SANAE/1579/1-579 ---GN-FIERGV---TAS-GLSHEKKWQFTPT--VN------AGDKVSG GSIIGTVPETKSIVHKVMVPPLT----GETA--IKDI-KAGEFTVDEVIG HLADG----TELRLLQKWPVRKPRP------FKEKLRPDIPLVTGQRIL 70Nitrosococcus_halophilus_Nc4/1593/1-593 ---GD-RISRGI---QLQ-GLDQERVWHFQPNPQLT------TGMTVTG GVQLGAVPETPTIEHRILVPPEL-----SGE--LLELVGEGDYPLAEVIA RVRTRDNRLHDLTLSHRWPVRKFRP------YRQREYSVAPLITGQRIL 229Thermococcus_sibiricus_MM_739/1585/1-585 ---GD-FIGRGL---TAP-ALSRDKKWHFTPK--VK------VGDKVVG GDIIGVVPETSIIEHKIMIPPEI-----EGE--IIEIVGEGDYTIEEVIA KVKAPNGEIKEVRMYQRWPVRMKRP------YKQKLPPEVPLVTGQRTI 175Clostridium_perfringens_SM101/1591/1-591 --NSA-FLSKGV---EVK-SLNREKKWPFVPT--AK------VGDKVSA GDVIGTVQETAVVLHRIMVPFGV-----EGT--IKEI-KAGDFNVEEVIA VVETEKGD-KNLTLMQKWPVRKGRP------YARKLNPVEPMTTGQRVI 179Clostridium_tetani_E88/1592/1-592 ---GD-FITRGI---DVP-SLSREKVWHFNPT--KK------AGDKVET GDILGLVQETSVIEHRIMVPPGI-----KGE--IISL-NEGDYTVIDKIG EIKTDKGI-EDLTLMQKWPVRRGRP------YKRKLNPSAPMVTGQRVV 107Aeropyrum_pernix_K1/1597/1-597 PRRRM-FVERGI---QAP-PLPRDRKFHFKPE-PLK------EGDKVEG GDALGRVPETSLIEHVVMVPPGI-----RGR--LKWLASEGDYSVEDTIA VVERDGRD-VEIRMHQRWPVRIPRP------FKEKLEPQLPLITGVRII 105Ignicoccus_hospitalis_KIN4/I/1596/1-596 --KSP-FIKRGI---KVP-SLDRSKKWEWRPNPELK------PGDKVSG DDILGTVPETPLIEHKVMVPPNVVPVDKAAT--LKWLAPAGEYTIEDTIA VVEYEGKE-IELKMYHRWPIRRPRP------VKEKFEPVTPLITGVRVL 301Thetis_Sea/1-587/1-751 ---GS-FIDRGV---QTN-PLPRDKEWTFEPA-DLD------EGEEVEP GDVLGKVDETTLVEHKIMVPPGV-----SGE--LKELVGRAEYTITDMIA TIQTEDGE-EEVQMLQEWPVREPRP------YDDKLAPEIPLISGQRIM 31Desulfarculus_baarsii_DSM_2075/1581/1-581 ---GL-FLKRGV---YID-PLDRQVKWDFTPK--AK------VGDVVGA GDMLGSVPEGK-FEHKIFVPFNF-----IGKAKIVEMAAKGDYTVVDTIA VVEDQSGARQNLCMMQRWPIKIALK------DYEERLLPVEPLVTGCRII 158Clostridium_phytofermentans_ISDg/1589/1-589 ---GN-NLERGI---EVP-SLNRDRKWNFEPT--AY------VGLEVST GDILGTTMESPIVIHKVLVPNGV-----SGI--ISKI-FNGEFTVEETIA VIKTENGEEVPVSMLQKWPVRIGRP------FKEKLAPQMPLITGQRVI 193Halobacterium_salinarum_R1/1585/1-585 ---GA-FLDRGV---DAP-GIDLDTDWEFEPT--VE------AGDEVAA GDVVGTVDETVSIEHKVLVPPRS----DGGE--VVAV-ESGTFTVDDTVV ELDTG----EEIQMHQEWPVRRQRP------TVDKQTPTEPLVSGQRIL 46Spirochaeta_thermophila_DSM_6192/1591/1-591 ---GF-FLERGV---YVD-PLDPEKRWAFTPV--AK------PGDVVTG GDTLGTVPEGI-FQHRIMVPFNM-----REQYTVRRVVPAGDYTVNDVVA EVEDPQGEVYPLTMSFYWPVKVPIT------AYAERLQPTEPMVTKIRII 196Halorhabdus_utahensis_DSM_12940/1587/1-587 ---NSAFLDRGV---DAP-GIDIEKTWEFTPE--VE------AGEEVTA GDIVGTVPETESIDHKVMVPPDY----DGGE--VIDT-KKGNFTVEETVV ELDTG----EEIQMRQEWPVRQARP------AGDKETPTDPLVTGQRIL 12Legionella_longbeachae_NSW150/1603/1-603 ---GF-FLPRGV---REH-AIDPEKKWVFSAA--VR------ADDTVHG GGILGVVQEGR-FTHKIMVPFDM-----GQDIHVEWI-QQGTFTADTPIA KLIDLENNVREVSMLQKWPVRFPITQFLMKNELIERLYPSEPMITTLRLI 236Methanocaldococcus_infernus_ME/1589/1-589 ---GSIFIPRGV---DVP-ALPREIKWEFKPV--VN------EGDLVEG GDVIGLVDETPSITHKIMVPFGI-----KGK--IVEI-KEGKFTVEETVA TVELENGETREIKMMQKWPVRKGRP------YREKLPPKIPLITGQRVE 300Thermococcus_litoralis_DSM_5473]/1585/1-585 ---GD-FIGRGI---TAP-ALPRDKKWHFTSK--VK------VGDKVVS GDIIGVVPETSIIEHKIMVPPGI-----EGE--ILEIAEEGDYTIEEVIA KVKTPDGEIKELKMYQRWPVRVKRP------YKEKLPPEVPLITGQRTI 231Pyrococcus_horikoshii_OT3/1964/1-588 ---GD-FIARGV---TAP-ALPRDKKWHFIPK--AK------VGDKVVG GDIIGEVPETSIIVHKIMVPPGI-----EGE--IVEIAEEGDYTIEEVIA KVKTPSGEIKELKMYQRWPVRVKRP------YKEKLPPEVPLITGQRVI 73Anaeromyxobacter_sp._Fw1095/1578/1-578 ---GD-FLGRGA---RLP-ALDRARRWELEPS--VR------RGDDLVP GACLGVARS-GAEAQPLLAPPHV-----RGR--IAEV-RSGALAVDEPVV RLADG----AEIALLQRWPVRRPRP------IRRRLAPDVPFLTGQRVL 194Halalkalicoccus_jeotgali_B3/1587/1-587 ---GA-FLDRGV---DAP-GIDLEKTWDFTPE--VS------EGDTVEP GDVLGTVPETKSVDHKVMVPPDS----EGGE--VTAI-EAGEFDVTETVV TLDSG----EEIAMRQEWPVREARP------TANKRSPDRPLVTGQRVQ 202Haloterrigena_turkmenica_DSM_5511/1587/1-587 ---GTAFLDRGV---DAP-GIDLEKQWEFTPK--VE------PGDEVEP GDIVGVVEETVTIDHKVMVPPDY----EGGE--VTTV-GDGEFTVEETVV ELDNG----EEIQMHQEWPVREARP------AGDKETPTEPLVTGQRVQ 997Methanothermus_fervidus_DSM_2088/1585/1-585 ---GE-FIRRGV---DVP-SLPREKKWKFKPT--VD------VGDEVVG GDIIGEVQETKSIVHKIMVPPNI-----SGS--IENI-EEGKFKVDEKIA EIKTDDSV-EELKMMQKWPVRKSRP------YKKKLDPDVPLITGQRVQ 802Parvarchaeum/1583/1-583 ---GT-FISSGV---KVN-SLDREKKWHFKPK--VE------EGKIVEP GEEIGEVKETSLIKHKVLVPANK-----KGV--IKKIAEEGDYTIEENIA ILEDNNKT-EEIKLMTRWPVRTARP------VKEKLSPNIPLLTGQRVI 208Methanosarcina_barkeri_str._Fusaro/1578/1-578 ---GG-FIGRGV---TAD-GLDHKKLWEFKPV--AK------KGDSVKG GDVIGVVQETVNIEHKIMVPPDI-----SGT--ISDI-KSGNFTVVDTIC TLTDG----TELQMMQKWPVRRPRP------VRTKLTPTRPLVTGMRIL Ferroplasma_sp._Type_II ---GD-FIRRGY---TAN-PIDLKAKWEFKPL--VK------KGDDIKP GQILGTVQETSLIEHKIMAPRNM-----EGQ--ISEI-LEGSMTVTDVYA HVKTKNGD-KKLTMRQEWPVRTIRG------VKSKLPPSIPLVTGQRVI 84Caldivirga_maquilingensis_IC167/1595/1-595 ---GRPFIARGINYDRAP-PLDFSRKWRWIPK--VK------IGDKVNV GTVLGIVPETQLIEHRILYPPLY----KPGI--IKYIAPEGDYTLNDDIA EVETSDGL-IKVKMWHKWPVRRPRP------FQEKLPPSDPLITGIRVI 197Haloquadratum_walsbyi_DSM_16790/1585/1-585 ---GSPYLDRGV---DAP-GIELDTEWEFEPT--VS------EGDTVES GDEVGIVEETVTIDHKVLVPPDY----EGGE--VTTV-KSGEFTVEETVV ELSSG----ESVQMRQEWPVREPRP------TVEKKTPREPLVSGQRIL 232Pyrococcus_abyssi_GE5/11017/1-588 ---GD-FIARGV---TAP-ALPRDKKWHFIPK--VK------VGDKVVG GDIIGEVPETSIITHKIMVPPGI-----EGE--IVEIAEEGEYTIEEVIA KVKTPSGEIKELKMYQRWPVRVKRP------YKEKLPPEVPLITGQRVI 200Halomicrobium_mukohataei_DSM_12286/1586/1-586 ---GSPYLDRGV---DAP-GIDLEKTWEFEPE--VE------AGDEVSS GDIVGTVPETPSIDHKVMVPPDS----DGGE--VVAA-ESGNFTVEEPVV ELDTG----EEIQMRQEWPVRQQRP------SEEKQTPTTPLISGQRVL 143Streptococcus_sanguinis_SK36/1623/1-623 --ASD-FLVRGV---QLP-NLDREAKWDFVPG--LS------VGDAVEA GDVLGTVQETNLVEHRIMVPVGV-----SGR--LANI-SAGSFTVEETVY EIEQADGSVFKGTLMQKWPVRRGRP------FAQKLIPVEPLVTGQRVI 30Waddlia_chondrophila_WSU_861044/1596/1-596 ---GL-FLPRGI---YLN-ALDRDKNWEYESK--VK------VGDVVKR GDTLGVTHEGR-FRHYVMVPFKN-----FGEYTITWVIDKGSYNIDTVVA KGKDKNGNEHRFTMVQKWPVKTGLM------QGEKIRPTEMMDTGCRIL 45Spirochaeta_smaragdinae_DSM_11293/1588/1-588 ---GF-FLQRGV---YLS-ALPRDKKWDFSPK--VK------VGDSVTA ADTLGTVPEGI-FEHRIMVPFDK-----QGSYRIKKMESAGSYDIDHEIA VMTDEKGNDIPLTMSFHWPVKRAIS------CFDERLKPTKPMITKVRLI 131Thermanaerovibrio_acidaminovorans_DSM_6589/1569/1-569 --KSP-YISRGI---SVP-AVDRHRKWFFEPK--VK------EGDQVSS GDVLGVVKETVLVEHRILVPYGV-----SGQ--VVSI-KAGDFTVEEVVA VIRTSEGD-REVSMLQRWPVRVPRP------VAKRLPPDTPLSTGQRVV 42Treponema_pallidum_subsp._pallidum_str._Nichols/1589/1-589 ---GY-FLERGV---YLP-ALSRTSEWMFTPH--VS------VGERVVR GDVLGYTPEGA-LKHRIMVPFHM-----GDSYEVVFIQTAGTYRVHDVIA RVRDAQGHEHELTMAFRWPVKRPVH------CYAERLKPTEPLVTSIRTI 77Thermofilum_pendens_Hrk_5/1601/1-601 ---GP-FVKRGV---KVN-PLPRNVKWHFKPS--VK------VGDKVSS GDIIGVVQETPLIEHRVMVPIGV-----SGK--VKEVVPEGDYTVEDPVV ILESDGRV-VELTMKQEWPVRQPRP------YKERLPSEVPLLIGQRII 110Thermus_thermophilus_HB27/1578/1-578 ---GI-YITRGV---VVH-ALDREKKWAWTPM--VK------PGDEVRG GMVLGTVPEFS-FTHKILVPPDV-----RGR--VKEVKPAGEYTVEEPVV VLEDG----TELKMYHTWPVRRARP------VQRKLDPNTPFLTGMRIL 203Natrialba_magadii_ATCC_43099/1587/1-587 ---GTAFLDRGV---DAP-GIDFETEWEFTPT--VD------VGDTVEA GDIVGEVPETESITHKVMVPPDY----EGGE--IESI-ESGAFTVDEVIA ELSSG----EEITMHQEWPVRQARP------ADTKETPTVPLVSGQRIL 93Sulfolobus_acidocaldarius_DSM_639/1592/1-592 --KSP-FVTRGV---KVP-ALERNKKWHVIPV--AK------KGDKVSP GDIIAKVNETDLIEHRIIVPPNV-----HGT--LKEISPEGDYTVEDVIA RVDMEGDV-KELKLYQRWPVRIPRP------FKEKLEPTEPLLTGTRVV 35Borrelia_burgdorferi_B31/1575/1-575 ---GF-FLERGV---YLR-PLNKDKKWNFKKT--SK------VGDIVIA GDFLGFVIEGT-VHHQIMIPFYK-----RDSYKIVEIVSDGDYSIDEQIA VIEDDSGMRHNITMSFHWPVKVPIT------NYKERLIPSEPMLTQTRII 55Spirochaeta_smaragdinae_DSM_11293/1592/1-592 ---GT-YIERGI---DEP-ALDRKKKWTFAPD--SK------VGDDISG GMILGRVKETDRVEHRIMAHPDI-----SGR--IVNIAEKGEYAIDEVIA EIEDESGNKHSLTMVQRWPIRIPRP------SKSRKPLTIPLITGQRVI 29Candidatus_Protochlamydia_amoebophila_UWE25/1593/1-593 ---GL-FLPKGI---YLS-ALDRQRKWDFESS--AK------VGDVLFR GDRIGSTKEGR-FHHFIMVPFSL-----YGKYRLTWVINSGSYTVDTVVA KAIDESGQEHSFTMVQKWPVKNALI------HGEKIKPTKMMDTGERII 198Haloferax_volcanii_DS2/1586/1-586 ---DSAFLDRGV---DAP-GIDLDEKWEFEPT--VS------EGDEVAP GDVVGTVPETVTIEHKVMVPPDF----GGGE--VVAV-EEGEFSVTEAVV ELDSG----EEITMHQEWPVRQARP------AAEKKTPREPLVSGQRIL 302Thermoplasmatales_archaeon/1-575 ---GD-FIKRGL---YPD-PVDLKKKWKFVPS--VK------MGESVTA GDVIGTVQETSIIEHRIMVPPGM-----EGV--IADI-SEGQFGPVETVA HLSSKSGS-REVSMGQYWPVRSPRP------VVRKLPPEIPLITGQRVI 62Halothermothrix_orenii_H_168/1590/1-590 ---GS-FIKRGI---EVN-PLDRDREWTVSVK--VK------LGDRVKP GQVVAEVPETSIVTHRVMVPPHL-----SGE--VVEVVPNGKYTVDQEIV TVKDDKGNNHQIRLHQKWPVRRPRP------CGERLPIKKPLLTGQRVF 115Truepera_radiovictrix_DSM_17093/1579/1-579 ---GT-YIDRGI---TVN-SLSRETKWAFTPQ--VS------VGDEVKG GQAIGTVPEFS-FTHKILVPPNV-----SGK--IKTIVGKGEYTVEDVIA TLEDG----TELKLYHAWPVRQARP------VQQKLDPVTPFLTGMRIL 16Chlamydophila_pneumoniae_CWL029/1591/1-591 ---SS-FLQRGK---HVN-AISDHNLWNYTPV--AS------VGDTLRR GDLLGTVPEGR-FTHKIMVPFSC-----FQEVTLTWVISEGTYNAHTVVA KARDAQGKECAFTMVQRWPIKQAFI------EGEKIPAHKIMDVGLRIL 213Methanohalophilus_mahii_DSM_5219/1576/1-576 ---GD-FIERGV---TAN-GLTREKKWEFTPT--VS------KGDSVKG GDIIGVVQETPNLEHKIMVPPRK-----SGT--VEDI-KSGKFVVDETVC VLSDG----SELSLMQKWPVRGPRP------VGKKLSPTRPLVTGQRIL 78Candidatus_Korarchaeum_cryptofilum_OPF8/1595/1-595 --GGP-FIVRGI---KSP-TLPRDRKWHWIPQ--VK------RGDLIQS GDVLGKVKEGP-IDHKIMLPPNI----MRAR--VLETYPEGDYTIEEGIV LIEHVGGK-EELKMYQKWPVRNPRP------FKEKLDPSELLITGQRVI 19Chlamydia_muridarum_Nigg/1591/1-591 ---SL-FLKRGE---YVN-AICRETVWAYTQK--AS------VGDVLSR GDVLGTVKEGR-FDHKIMVPFSC-----FEEVTITWVISSGDYTVDTVIA KGRTASGAELEFTMVQKWPIKQAFL------EGEKVPSHEIMDVGLRVL 52Thermanaerovibrio_acidaminovorans_DSM_6589/1594/1-594 ---GI-YITPGA---QVE-QLDLDARWDVTVT--AR------RGQMVSP GTVVAEVQETPLMVHRIMVPPGV-----EGE--VVEVQPSGSLRTRDWVV KVADGSGRVTPVHLAQSWPVRMPRP------YRERLLPNEPLVTGQRVI 223Methanopyrus_kandleri_AV19/1592/1-592 ---GD-FVERGI---LVS-ALDRKKKWEFTPK--VK------EGEKVEE GDVLGTVPETEFIEHKIMVPPGV-----SGE--VIEIAADGEYTVEDTIA VIEDEEGEEHEVTMMQEWPVRKPRP------YKRKLDPEEPLITGQRVI 189Leptotrichia_buccalis_DSM_1135/1597/1-597 ---GD-FLDKGV---EVN-PLNRDKKWEFEPV--LS------TGAEVET GDILGTVQETSVVSHKIMVPAGI-----KGT--LKTI-KSGSYTVADTIA VIETEKGELIEVQMMQKWPVRRGRK------YKQKLNPEAPLITGQRVI 191Fusobacterium_nucleatum_subsp._nucleatum_ATCC_25586/1382/1-584 ---GD-FLLKGV---SVP-ALDREKKWQFTPT--MQ------VGEEVEP GKVIGTVQETEIVLHKIMVPNGV-----YGK--IIDI-KEGEFTVDKTIC SIETENGV-RELNMIQKWPVRKGRP------YLRKL-----MITGQRII 4Bacteroides_vulgatus_ATCC_8482/1584/1-584 ---GV-FLKRGQ---YTY-PLDKERVWHFVPL--VN------VGDEVGP SAWLGQVDENF-QPLKIMVPFQL-----TGTYKVKSIVPEGDYTIEDTVV VLTDREGKDIPVNMIQKWPVKKAMT------NYKEKPRPYKLLETGVRVI 95Metallosphaera_sedula_DSM_5348/1591/1-591 --KSP-FIKRGI---KLP-TLNREKEWHFVPK--MK------KGDKVEP GDILGVVQETGLVEHRILVPPYV-----HGK--LKEVVAEGDYKVEDNVA IVDMNGDE-VPVKMMQKWPVRVPRP------FKEKLDPSQPLLTGVRIL 44Treponema_denticola_ATCC_35405/1589/1-589 ---GY-FLERGI---YLN-ALSRTAKWHFTPS--AK------EGDTLKR ADLLGTVPEGS-FTHRIMIPFNM-----YGTYKLKSIKPEGDYTVDDTIA EVTDERGNVIPLTMSFKWPVKRAID------CYAERLKPTETLVTKMRTM 90Desulfurococcus_kamchatkensis_1221n/1584/1-584 ---GI-FVRKGV---RTT-PLPRDKKWHFIPL--VK------PGDKVVE GDFIGYVNETEVVKHYIMVPPGI-----NGV--IEWVASEGDYSIVEPIA KISG-----KEVSMLQKWPVRRPRP------YKEKLEPREPFITGIRVI 57Thermotoga_sp._RQ2/1586/1-586 ---GP-FIGRGI---QAF-GLDTEKFWSVNVL--KK------EGDRVRG GEVIAEVQETGSVKHKIMIPPHI----KEGT--LLNVKQSGDYKVTDTIA VVKTENGE-EEIKLYQEWPVRVPRP------VKRFLELSIPLITGQRVI 803Pyrococcus_yayanosii/1587/1-587 ---GD-FIGRGL---TAP-ALPRDKKWHFTPK--VK------VGDKVVG GDIIGEVPETSLIVHKIMVPPGI-----EGE--IIEIAEEGEYTVEEVIA KVKTPSGEIKELKMYQRWPVRVKRP------YKEKLPPEVPLITGQRVI 228Methanobrevibacter_smithii_ATCC_35061/1584/1-584 ---GD-FIARGV---DAE-SISKEKKWTFKPV--AK------VGDKVKG GDVLGEVQETSAVLQKILVPPMI-----EGE--LTSIASEGEYTVLEDIA EVATDKGD-EKIQMLQKWPVRKGRP------YVDKLDPDVPLVTGQRAQ 13Cyanothece_sp._PCC_8801/1607/1-607 ---GT-FLPRGV---YVS-PLNTQKKWSFVPT--AK------IGDRLKA GDVIGTVAEGR-FTHKIMIPFDE-----PTEVTIDWI-QQGNVTIDTPIA RIKDKNGQERSITLTQSWPVRRPLPQELLTKRYSERLYPQEPMITTQRII 168Thermoanaerobacter_pseudethanolicus_ATCC_33223/1590/1-590 ---GS-FITRGI---DVP-SLNREKKWKFTPK--VK------SGDKVSG GDIIGTVQETVIVEHRIMVPPAI-----SGI--VEDI-REGEYTVTEPIA RIKTDSGQIVEITMMQKWPVRKARP------YKEKLPPEIPMPTGQRVI 26Chlamydophila_felis_Fe/C56/1588/1-588 ---SF-FLKRGE---YVN-ALCRKTLWEYTPT--AV------VGDVLVR GDALGVVKEGN-FNHKIMVPFSC-----FKEVTITWVISEGEYGVDTVIA KARDADGKEYSFTMVQKWPIKQAFI------QGDKVPSHEIMDVGVRIL 80Thermoproteus_neutrophilus_V24Sta/1594/1-594 ---KSPFIARGIGYDQAP-PLDLKAEFDFKPA--VK------PGEEVSP GDVLGSVKETELMTHYILYPPLP--ENAPGV--VEWV-ADGKYKVDDVIA RIKTKRGI-VEVKMWHKWPVRRPRP------FREKLPPVEPLITGVRTI 114Deinococcus_radiodurans_R1/1582/1-582 ---GN-FIARGI---EVS-SLNREQKWDFTPS--VQ------AGDTVTG SGILGTVPEFS-FTHKILVPPEV-----QGR--LRSVAPAGQYTIDDTIA ELEDG----TKLRLAHYWPVRAPRP------VQKKLDPSLPFLTGMRIL 122Methanocella_paludicola_SANAE/1579/1-579 ---GS-FISRGI---SVP-GLDRKKKWAFTPS--AK------KGDRLSP GDVIGMVPEFH-IEHRILVPPGV-----SGT--VAEI-KEGDLAVEDVVC TLEGG----RDIRLMHEWPVRKGRP------YRKKLDTSLPLITGQRVF 217Methanoplanus_petrolearius_DSM_11571/1582/1-582 ---GS-FIERGV---SAP-GLDHAKKWEFKPL--KK------KGDEVVP GEIIGEVQETN-IVTKIMIPPTF----KGGK--IKEI-KKGEFTVDEIVC VLDSG----EEVAMMQKWPVRVPRP------VTEKKNPDIPLITGQRIL 998Thermoplasma_acidophilum_1221B2/1590/1-590 ---GD-FIARGL---NPP-ALDRQKKWEFVPA--VK------KGETVFP GQILGTVQETSLITHRIMVPEGI-----SGK--VTMI-ADGEHRVEDVIA TVSGNGKS-YDIQMMTTWPVRKARR------VQRKLLSRDPLVTAQSGN 204Methanosaeta_thermophila_PT/1576/1-576 ---GD-FISRGL---FVD-GVDHKKKWEFKPL--VK------KGDTVKP GQPIGEVQEQLLIKHKIMVPPKH----KGGV--VKEI-YSGNFTVEETVC VLEDG----SELTMLQKWPVRQARP------VVRKLPPTIPLRTGQRVI 218Methanospirillum_hungatei_JF1/1582/1-582 ---GN-FIERGV---TAP-GLSHDKKWEFKPI--KK------AGDMVTP GTIIGEVQETN-IVHKIMVPPYC----DAGK--IKDI-KSGSFTIDEIIC TLDNG----AEIAMMHKWPVRMPRP------VTEKLNPDIPLITGQRIL 221Candidatus_Methanosphaerula_palustris_E19c/1588/1-588 ---GN-FIERGV---SAP-GISREKKWEFKPL--AR------VGDQVSP GAIIGEVQETN-IVHKIMVPPNT----KAGV--ITTI-TPGTFTVEEVVC VLDNG----AELTMMQRWPVRIPRP------VAEKMNPTIPLITGQRIL 171Anaerococcus_prevotii_DSM_20548/1585/1-585 ---GE-FLERGV---TST-ALDREKTWEFNPT--AS------VSDKVES GDILGEVEETSVITHKIMVPIGI-----SGT--IKDI-KAGEFTVDETIA IIETEDGD-KEVSMLQKWPVRQARP------SRRKIDPDEPLITGQRVI 120Ferroplasma_acidarmanus_fer1/1589/1-589 ---GD-FIVRGA---TAP-PLDEKKEWDFVPL--LK------EGDEVEQ GYILGTVQETNIITHKIMVPYGI-----RGI--IKSI-SKGKFKVSDTVC MIDTGTSK-YDIKLKQIWPVRQARK------VFLKFAPEIPLITGQRVI 119Thermoplasma_volcanium_GSS1/1776/1-590 ---GD-FIARGL---NPP-PLDRKKEWDFVPA--VK------KNDIVYP GQVIGTVQETSLITHRIIVPDGV-----SGK--IKSI-YEGKRTVEDVVC TISTEHGD-VDVNLMTTWPVRKARR------VVRKLPPEIPLVTGQRVI 3Brachyspira_murdochii_DSM_12563/1587/1-587 ---GV-FLERGK---YNNLSEDKESTYDFTPI--AK------AGDEVAA GDWIGAVKEGW-IDHKIMVPFKF-----QGKGVVESVASAGTYHLQDTLA VIKTANGEKVNVTMIQTWPVKLTIK------AYKEKPRPFKLLETGVRTI 59Anaerococcus_prevotii_DSM_20548/1582/1-582 ---GS-FIPSGI---GFE-NLDTEKKWDVKLT--VK------VGDKIKR GDIYATIKETETIEHRLMT--QV-----SGE--VVEVAEDGLYTLEDTVV KIKTEKNV-VEEKLYQYWPVRNQRP------VMANMPIEKLFETGQRVL 164Clostridium_thermocellum_ATCC_27405/1589/1-589 ---GS-NITRGI---DVT-ALDRSKKWDFQPT--VK------KGDKVTA GDVIGKVQETSIVEHRIMVPYGV-----QGT--IEEI-KSGSFTVEETVA KVRTENNELVDICMMQKWPVRIGRP------YREKLPPNAPLVTGQRVI 237Methanocaldococcus_vulcanius_M7/1587/1-587 ---GSIFIPRGV---DVP-SLPRDVKWEFKPT--VS------EGDVVEE GDIIGTVEETPSIVHKILVPIGV-----KGK--IVEI-KEGKFTVEETVA VVEMENGERKDIIMMQKWPVRKPRP------YKEKLPPKIPLITGQRVE 124Dictyoglomus_thermophilum_H612/1589/1-589 ---GD-FLSRGL---SFP-ALDREKKWDFEAR--VK------PGDYVEG GDVLGIVQETSALEHRILVPPYL-----RGK--ILEI-KSGTYTVEEVIG VLEDDSGKKHELKLMHKWPVRYQRP------VKEKLPPQVPLITGQRVL 153Streptococcus_pyogenes_M1_GAS/1591/1-591 --DSD-FLIRGV---AIP-SLDRKAKWAFIPK--LS------VGQEVVA GDILGTVQETAVIEHRIMVPYKV-----SGT--LVAI-HAGDFTVTDTVY EIKQEDGSIYQGSLMQTWPVRQSRP------VAQKLIPVEPLVTGQRVI 41Fibrobacter_succinogenes_subsp._succinogenes_S85/1585/1-585 ---GF-FLQRGK---YLK-ALPRDKKWAFTPV--AK------AGDVVVA GDTLGTVPEGV-FSHRIMVPFKL-----LGKWTVDYITTAGDRVVEDVVA KLKNDKGETVDVTMVQTWPVKMPIK------AFEERLRPSKPLTMQQRII 220Candidatus_Methanoregula_boonei_6A8/1588/1-588 ---GN-FIERGV---SAP-GLSHEKKWTFKPV--VK------AGDKVEP GAILGEVQETN-IVHKVMLPPNV----KAGV--VKTI-KAGDFTVDEIIV ILEDG----REYPMIQRWPVRVPRP------VKEKKNPTIPLLTGQRIL 79Pyrobaculum_islandicum_DSM_4184/1593/1-593 ---KSPFIARGVGYDQAP-PLDLKAEFDFKPN--VK------PGEEVYP GDVLGSVKETELITHYILYPPLP--EYVPGT--VEWI-ADGKYKVDDVIA RIKTKRGV-IEVKMWHKWPVRRPRP------FREKLPPVEPLITGVRTI 177Clostridium_botulinum_E3_str._Alaska_E43/1592/1-592 --KSS-FLTRGV---SVP-SLNREKKWDFKPT--AK------VGDEVKS GDVIGTVQETPVVEQRIMIPIGI-----EGK--IKEI-NAGSFTVTETIA IVETEKGD-REVQLMQKWPVRKGRP------YSAKINPVEPMLTGQRVI 48Geobacter_uraniireducens_Rf4/1524/1-524 ---GP-FICSGS---DLY-PLELERPWRFFPL--RR------AGDEVVA SDIIGYVEEGA-FRHPLTA--GA-----SGA--IARL-EDGEFNLTQPVG AFADG----REITAIREWPVRKPRP------YRKKLSPDLPLVTGQRAV 211Methanohalobium_evestigatum_Z7303/1578/1-578 ---GD-FIARGA---TAN-GIDREKQWEFKPI--VD------KGDKVEP GDIIGVVQETPNLEHQIMVPPNM----QGGT--VSDI-YSGKFTVDETVC VLSNG----KELSMLQKWPVRTPRP------TKRKLKPDKPLLTGQRIL 230Pyrococcus_furiosus_DSM_3638/11013/1-588 ---GH-FIARGI---SAP-ALPRDKKWHFTPK--VK------VGDKVVG GDIIGEVPETSIIVHKIMVPPGI-----EGE--IVEIADEGEYTIEEVIA KVKTPSGEIKELKMYQRWPVRVKRP------YKEKLPPEVPLVTGQRVI 47Stackebrandtia_nassauensis_DSM_44728/1575/1-575 ------FLGRGT---AAP-SFG-SRPWSFDPL--AT------VGARVGP GDELGVLRDSRPVKHPVLVPPTL-----AGE--LEYLAPEGRYPAETVIA RVAG-----QPVHMAQPWPVRRPRP------VRNRLEQTAPLHTGQRVL 199Haloarcula_marismortui_ATCC_43049/1586/1-586 ---GSAFLDRGV---DAP-GIDLEKTWEFTPE--VE------EGDEVEA GDIVGTVPETPSIDHKVMVPPDS----EGGE--VVAI-ESGNFNVEETVV ELDNG----EEIQMHQEWPVRQQRP------TVEKETPTEPLISGQRVL 227Methanobrevibacter_ruminantium_M1/1584/1-584 ---GD-YIARGI---DVD-SIDKEKKWTFEPI--AK------VGDKVVG GDILGQVQETSAVVQKIMVPPMI-----EGT--IKSIASQAEYTVLETIA EIETEDGI-EEIQMLQKWPVRKGRP------YKEKLDPDIPLITGQRAQ 10Parabacteroides_distasonis_ATCC_8503/1585/1-585 ---GV-FLKRGD---YTF-ALDNDKKWDFKPL--AK------AGDTVSA GDWLGEVDENF-QPHKIMVPFKF-----EGSYTVKSVVPAGQYTINDTMA VLTDENGTDVNVTMIQKWPVKKAIT------CYKEKPRPFKLLETGVRTI 195Halorubrum_lacusprofundi_ATCC_49239/1591/1-591 ---GSPYLDRGV---DAP-GIDLEKKWEFEPT--VE------VGDEVGR GDVVGVVEETVTIDHKVMVPPDALEEDETTE--VTAI-EAGSFDVTETVA ELANG----TNVSMHQEWPVREARP------SENKKTPRTPLVSGQRIL 64Thermosediminibacter_oceani_DSM_16646/1587/1-587 ---SG-FIPPGI---GLI-SLDEEKLWDVEIL--IR------EGDHLKE GDVFARVQETSMIVHRIMVPPGL-----HGR--VVWAKESGRYTINDTLA LLENEGKT-FELKMCQEWPVREPRP------VAKRMPIGKLLVTGQRVI 144Streptococcus_gallolyticus_UCN34/1591/1-591 --NSD-FLIRGV---ELP-SLDREAKWDFVPS--LE------VGAEVST GDILGTVQETSVVEHRIMVPNNV-----SGK--LISI-TAGEFTVEETVY EIEQADGSIFKGTLLQKWPVRKGRP------VAQRLIPEEPLVTGQRVI 165Thermosediminibacter_oceani_DSM_16646/1590/1-590 ---GS-FITRGI---DVP-ALNREKKWGFTPR--VR------PGDRVTG GDIIGTVQETVIVEHRIMVPPGV-----SGV--VEDI-KEGEFTVTEPVA RIKTDSGQVVKVTMMQKWPVRKTRP------YKEKLPPEIPMSTGQRVI 172Clostridium_cellulovorans_743B/1591/1-591 ---GSNFLVKGI---SVE-PLDRDKRWEFVPS--VS------EGEAVSA GDVIGTVQETAVIVHKIMIPFGI-----SGT--VKSI-KAGDFTVKDPIA VISTASGD-KEILMMQKWPVRKGRP------IHKKLNPIEPMITGQRVI 201Natronomonas_pharaonis_DSM_2160/1585/1-585 ---GA-FLDRGV---DAP-GIDLEKTWEFNPE--VS------EGDEVSP GDVVGIVPETESIDHKVLVPPDY----EGGE--VTAV-ESGNFTVDETVV ELDSG----EEIQMRQEWPVREPRP------TVEKETPTTPLVSGQRVL 178Clostridium_novyi_NT/1591/1-591 ---GS-YLTKGI---EVF-SLNRDKKWHFVPK--VK------HSDKVKA GDILGTVQETEVVNHKIMVPYGI-----EGE--VITI-FEGDYTVEDVVC EIDTKDGV-KKVKLMQKWPVRKGRP------YAKKLNPEAPLVTGQRII 141Streptococcus_mitis_B6/1591/1-591 --HND-FLVRGV---EVP-SLDRDIKWHFDST--IA------IGQKVST GDILGTVKETEVVNHKIMVPYGV-----SGE--VVSI-ASGDFTIDEVVY EIKKLDGSFYKGTLMQKWPVRKARP------VSKRLIPEEPLITGQRVI 109Methanospirillum_hungatei_JF1/1588/1-588 ---GD-MIERGS---SAP-ALDRTKKYRFYPL--AK------SGDMVRE GDRIGCVRESVSVNHYILIPPGL-----SGT--IHFIAEQGEYVITDTIV ILDTGEEK-KELTMIQRWPVRDPRP------VRERLDPVEPLLTGQRII 49Aminobacterium_colombiense_DSM_12261/1588/1-588 ---SI-YLKKGI---HLP-AIDSRKKWYFTPS--IK------EGEIVTP GTFIGYVFEGSFLQHRIMAPPYI----EPAK--VVWVASEENYTIEDSIC RLENG----VELTMKQRWEVRRPRP------VLSRLSFDAPLLTGQRIL 118Thermoplasma_acidophilum_DSM_1728/1764/1-590 ---GD-FIARGL---NPP-ALDRQKKWEFVPA--VK------KGETVFP GQILGTVQETSLITHRIMVPEGI-----SGK--VTMI-ADGEHRVEDVIA TVSGNGKS-YDIQMMTTWPVRKARR------VQRKLPPEIPLVTGQRVI 94Sulfolobus_tokodaii_str._7/1595/1-595 --NSP-FVARGV---SIP-ALDRQTKWHFVPK--VK------SGDKVGP GDIIGVVQETDLIEHRILIPPNV-----HGT--LKELAREGDYTVEDVVA VVDMNGDE-IPVKMYQKWPVRIPRP------YKEKLEPVEPLLTGIRVL 102Sulfolobus_islandicus_M.16.27/1592/1-592 --KSP-FIARGI---KVP-SVDRKTKWHFIPK--VK------KGDKIEG GDIIGIVNETPLVEHRILVPPYV-----HGT--LKEIVAEGDYTVEDPIA VVDMNGDE-VPIKLMQRWPVRIPRP------FKEKLEPTEPLLTGTRVL 53Treponema_pallidum_subsp._pallidum_str._Nichols/1605/1-605 ---GA-FLRPGA---RSQ-PLDGSVRWDFRPH--CNERGEALCAGIPIAP GSVLGTVQETPSVVHTIMVPPDI-----RGSV-LSSFKGAGAYTIDEEIG RTDLG----EPLFLSQYWPVRRARP------FSKKLAVCEPLVTGQRAI 106Hyperthermus_butylicus_DSM_5456/1601/1-601 --KSI-FVRRGV---KVD-ALDRSRKWHFKPNTTLK------PGDKVSG GDVLGEVQETSLITHKVLVPPDV-----HGR--LKWLASEGDYTVEDVIA VVEADGKE-IELKMYHRWPVRRARP------IIEKLEPVEPLITGMRVI 104Acidilobus_saccharovorans_34515/1601/1-601 PARSI-FVERGV---TVP-PIPRDKKWHFTPSDRVK------PGDKVGP GDIIGVVEETQIIQHKILVPPNV-----EGT--VKWIAGEGDYTIVDPIA EIDVGGGEIKQLYLYQRWPVRQPRP------YVQKLEPTEPLITGMRII 58Acholeplasma_laidlawii_PG8A/1583/1-583 ---GP-FIKAGS---NIP-SLDFEQTFDVEVK--VQ------EGNYLGC GMVYAVIKETSAIEHRLMVPPHI-----EGI--AKSVKPSGTYKVKDTVL VLETKAGD-VELKLYQTWPIKKARP------VTERLELSTPLITGQRVI 130Aminobacterium_colombiense_DSM_12261/1598/1-598 --KSH-FISRGI---DVP-AIDRNKKWKFEPR--VK------AGDRVHA GDILGTVKETVLVEHHILVPYGV-----EGT--VSEI-KEGEFTVIETIA VIKNESGEEKNVVMLQRWPVRKPRP------VARRLPPIIPLTTGQRVV 243Methanococcus_vannielii_SB/1586/1-586 ---NSIYIPRGV---NVP-SLPRDVKWDFVPS--VN------VGDEVLA GDIIGTVQETASIVHKILIPVGI-----NGK--IKEI-KSGSFTVEETVA VVETEKGD-KLVTMMQKWPVRKPRP------SKVKLPPVIPLLTGQRVE 207Archaeoglobus_profundus_DSM_5631/1580/1-580 ---GD-FIGRGI---EAP-GLDRKKKWHFKPT--VK------KGDKVNG GEIIGTVQETSMIEHRILVPPNV-----SGT--IVEI-YEGDFTVEDTIA VLDNG----IELKMYHKWPVRIPRP------YKEKLPPEIPLITGQRIL 999Ferroplasma_acidarmanus/1589/1-589 ---GD-FIVRGA---TAP-SLDEEKEWNFTPI--LK------DGDEVEQ GYILGTVQETDIIVHKIMVPYGV-----NGI--IKNI-KSGKFRVSDTVC TIDSGNVK-HEIKLKQIWPVRQARK------VFLKFAPEIPLITGQRVI 116Meiothermus_ruber_DSM_1279/1578/1-578 ---GI-FISRGI---EVS-SLDRTRKWDFTPL--KK------VGDEVKG GDILGTVPEYS-FTHKILVPPDK-----AGR--IKHIVEAGQYTIDDTIA ELEDG----TKLRLAHFWPVRKPRP------FTRKLDPNQPFLTGMRIL 123Methanospirillum_hungatei_JF1/1584/1-584 ---GN-YISRGI---MVP-GLDREKRWAFNPV--KK------AGEMVQT GDVLGTVQEFHLV-HSIMVPPGV-----SGE--IKTI-ASGEFTVTDVVC TLADG----TEITMLQRWPVRKGRP------FVKRLDPEVPLLTGQRVF 226Methanothermobacter_thermautotrophicus_str._Delta_H/1584/1-584 ---GD-YIERGV---DVP-SLPKDKKWTFKPT--AR------EGQMVKG GDIIGEVEETSSITHRIMIPPNV-----EGK--LTMIAPQGEYTVLDDIA EVETESGT-EKIQMLQKWPVRKGRP------YKKKLDPDVPLVTGQRAQ 241Methanococcus_aeolicus_Nankai3/1586/1-586 ---KSIYIPRGV---SVP-SISREAKWDFTPT--VN------VGDKVEA GDIIGTVPETKSIVHKIMIPNGI-----SGT--IKEI-KSGSFTVVEPIA IVETENGEEKEIIMMQKWPVRNPRP------YKEKLPPEIPLVTGQRLE 234Thermococcus_gammatolerans_EJ3/1585/1-585 ---GD-FIARGL---TAP-ALPRDKKWHFTPT--VK------VGDRVTG GDILGVVPETSIIEHKILVPPWV-----EGE--IVEIAEEGDYTVEEVIA KVKKPDGTIEELKMYHRWPVRVKRP------YKNKLPPEVPLITGQRTI 224Methanosphaera_stadtmanae_DSM_3091/1585/1-585 ---GD-FIPRGI---DVP-ALDKVKEWEFKPT--AS------VGDKVNG GDIIGTVDETSAIVHKIMIPPKM-----SGT--IKSIVSQGKYNVTEDIA EVETENGI-ETVQMMQVWPVRVGRP------YTNKLDPDVPLITGQRAQ 233Thermococcus_onnurineus_NA1/1585/1-585 ---GD-FIARGL---TAP-ALPRDKKWHFTPK--VK------VGDKVVG GDILGVVPETGIIEHRVLVPPWV-----EGE--IVEIVEEGDYTIEEVIA KVKKPNGEIEELKMYHRWPVRVKRP------YKRKLPPEVPLITGQRTI 66Eubacterium_limosum_KIST612/1595/1-595 ---PV-FIPEGI---GLM-SIDEEKMWDYKAR--LS------VGDTVKP GQIFGTVQETLMIEHRLMVPPNV-----KGT--VVEAREDGSYNIETVLV VVEDERKRRHELKMYQEWPVRTPRP------TRFRREPEKPLVTGQRII 188Streptobacillus_moniliformis_DSM_12112/1588/1-588 ---GD-FLLKGV---EVN-ALDREKIWEFVAT--KS------IGDEVSS GYVLGTVQETEVITHKIMVPIGI-----EGK--IKEI-KSGNFTVEDTIA VIETVNGD-VNVNMIQRWPVRKGRK------YAKKINPDEPLITGQRVI 140Streptococcus_pneumoniae_TIGR4/1591/1-591 --HND-FLVRGV---EVP-SLDRDIKWHFDST--IA------IGQKVST GDILGTVKETEVVNHKIMVPYGV-----SGE--VVSI-ASGDFTIDEVVY EIKKLDGSFYKGTLMQKWPVRKARP------VSKRLIPEEPLITGQRVI 60Clostridium_phytofermentans_ISDg/1588/1-588 ---GA-FISRGV---NIP-SLDEKRLFQTHIT--VA------PGELVYG GKVIAEVPETPAITHKVMVPPHV-----EGK--VISVVSDGEYTIKDTIV VIKDRDGKEINLTMTQEWPIRISRP------IGKRFPASMPLVTGQRIL Position of vma-1b intein 3 301 25Chlamydia_trachomatis_B/TZ1A828/OT/1591/1-591 DTQIPVLKGGTFCTPGPFGAGKTVLQHHLSKYAAVDIVVLCACGERAGEV VEILQEFPHLTDPHTGQSLMHRTCIICNTSSMPVAARESSIYLGITIAEY YRQMGLHILLLADSTSRWAQALREISGRLEEIPGEEAFPAYLASRIAAFY 801Micrarchaeum/1586/1-586 DSLFPVAKGGTAAVPGPFGSGKTVIQHQLAKWSDSEIVVYVGCGERGNEM TEILTTFPELKDPKSGKPIMDRTILIANTSNMPVAAREASIYTGITIAEY YRDMGYSVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRKIAEFY 222Aciduliprofundum_boonei_T469/1582/1-582 DTFFPIAKGGTAAIPGGFGTGKTVTQHQLAKWSDADIVVYVGCGERGNEM TEVLEDFPKLKDPRTGEPLMNRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYHVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASRLAEFY 127Acholeplasma_laidlawii_PG8A/1592/1-592 DTLFPIAIGGIAAIPGPFGSGKTVVQHQLAKWADADIIVYIGCGERGNEM TDVLMEFPELKDPRTGQPLMKRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYKVAIMADSTSRWAEALREMSSRLEEMPGEEGYPAYLGSRIAEFY 85Vulcanisaeta_distributa_DSM_14429/1603/1-603 DTLFPIAIGGAAAIPGPFGSGKTVTIRTLAMYSEARLVFPVLCGERGNEA ADALQGLLKLVDPNTGRPLMERTTIIVNTSNMPVAAREASIYMGVTIAEY FRDQGYDAFLMADSTSRWAEAMREVALRIGEMPSEEGFPAYLPTRLAEFY 210Methanosarcina_mazei_Go1/1578/1-578 DGLFPVAKGGTAAIPGPFGSGKTVTQQQLSKWSDTEIVVYIGCGERGNEM ADVLWEFPELEDPQTGRPLMERTILVANTSNMPVAAREASVYTGMTLAEY FRDMGYDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLSARLAEFY 8Porphyromonas_gingivalis_ATCC_33277/1584/1-584 DTFNPIVEGGTGFIPGPFGTGKTVLQHAISKQAEADIVIIAACGERANEV VEIFAEFPHLNDPHTGRKLMERTIIIANTSNMPVASREASVYTAMTIAEY YRSMGLRVLMMADSTSRWAQALREMSNRLEELPGPDAFPMDLSAIVANFY 216Methanocorpusculum_labreanum_Z/1581/1-581 DGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEM TEVLTEFPELQDPKTGNSLMERTILIANTSNMPVAAREASVYTGITLAEY FRDMGYDVALMADSTSRWAEAMREICSRLEEMPGEEGYPAYLSSRLSEFY 212Methanococcoides_burtonii_DSM_6242/1578/1-578 DGMFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDTDIVVYIGCGERGNEM ADVLNEFPELEDPKTGRPLMERTVLIANTSNMPVAAREASVYTGITIAEY YRDMGYDVSLMADSSSRWAEAMREISSRLEEMPGEEGYPAYLSARLSEFY 242Methanococcus_voltae_A3/1585/1-585 DTFFGLAKGGASAIPGPFGSGKTVTQHQLAKWSDVDIVVYIGCGERGNEM TEVIEEFPHLDDLKTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEY FRDMGFGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLSSKLAQFY 132Enterococcus_faecalis_V583/1595/1-595 DTFFPITKGGAAAVPGPFGAGKTVVQHQIAKWADVDLVVYVGCGERGNEM TDVLNEFPELIDPTTGESLMNRTILIANTSNMPVAAREASIYTGITIAEY FRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRLAEYY 63Clostridium_sticklandii_DSM_519/1594/1-594 DIFFPIAKGGTAALPGGFGTGKTMTQHQLAKWSDADIIVYIGCGERGNEI TEVLEDFPKLIDPRSGKPLMERTILIANTSNMPVAAREASIYTGITMAEY FRDMGYHVAIMADSTSRWAEALREISGRLEEMPAEEGYPAYLPSRLAQFY 215uncultured_methanogenic_archaeon_RCI/1579/1-579 DGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEM TEVLAEFPHLTDPKTGNPLMDRTVLIANTSNMPVAAREASCYTGITIAEY YRDMGYGVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 121Picrophilus_torridus_DSM_9790/1922/1-589 DAFFPVAKGGTVAVPGPFGSGKTVIQHQLSKWSDSDIVVYVGCGERGNEM TEILSTFPELMDPKTGKPIMQRTVLIANTSNMPVAAREASIYTGVTIAEY YRDMGYNVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRISEFY 209Methanosarcina_acetivorans_C2A/1578/1-578 DGLFPVAKGGTAAIPGPFGSGKTVTQQQLSKWSDTEIVVYVGCGERGNEM ADVLWDFPELEDPQTGRPLMERTILVANTSNMPVAAREASVYTGMTLAEY FRDMGYNVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLSARLAEFY 128Acidaminococcus_fermentans_DSM_20731/1590/1-590 DTLFPIAKGGVAAVPGPFGSGKTVIQHQLAKWADADIIVYVGCGERGNEM TDVLNEFPALNDPRTGLPLMKRTVLIANTSDMPVAAREASIYTGITIAEY YRDMGYKVAIMADSTSRWAEALREMSSRLEEMPGEEGYPAYLSSRLAEYY 5Bacteroides_fragilis_NCTC_9343/1585/1-585 DTVNPIVEGGTGFIPGPFGTGKTVLQHAISKQAEADIVIIAACGERANEV VEIFTEFPELVDPHTGRKLMERTIIIANTSNMPVAAREASVYTAMTIAEY YRAMGLKVLLMADSTSRWAQALREMSNRMEELPGPDAFPMDLSSIISNFY 96Sulfolobus_solfataricus_P2/1592/1-592 DTIFPIAKGGTAAIPGPFGSGKTVTLQSLAKWSAAKIVIYVGCGERGNEM TDELRQFPSLKDPWTGRPLLERTILVANTSNMPVAAREASIYVGITMAEY FRDQGYDTLLVADSTSRWAEALRDLGGRMEEMPAEEGFPSYLPSRLAEYY 75Anaeromyxobacter_dehalogenans_2CP1/1579/1-579 DCFFPVSAGGTAVVPGGFGTGKTVLEQSLAKWAAADVVVYVGCGERGNEM SEVLDEFPRLEDPRTGGPLLARTVMIVNTSNMPVAAREASIYTGCAIAEY FRDMGRSVALMIDSTSRWAEALREISARLEEMPGEEGYPTYLASRLARFY 235Thermococcus_kodakarensis_KOD1/1585/1-585 DTFFSQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVVYIGCGERGNEM TDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDQGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKIAEFY 92Nitrosopumilus_maritimus_SCM1/1592/1-592 DTFFPIAKGGTGSIPGAFGTGKTVTLHQIAKWADSQVVVYIGCGERGNEM TEVLVEFPHLKDPRSGKPLMDRTVLVANTSNMPVAAREASIYTGVTIAEY YRDMGKDVVLVADSTSRWAEALREMSGRLEEMPAEEGYPSYLASRLAEFY 190Finegoldia_magna_ATCC_29328/1587/1-587 DTFFPVAKGGTAAIPGPFGSGKTVVQHQLAKWADAEIVVYVGCGERGNEM TDVLNEFPELIDPKTGQSLMKRTVLIANTSNMPVAAREASIYTGITIAEY YRDMGYSVAMMADSTSRWAEALREMSGRLEEMPGDEGYPAYLASRIADFY 999Nanosalinarum_sp._J07AB56/1584/1-584 DGLFPLAKGGTAAVPGGFGTGKTVTQQSLAKFSDADVIVYIGCGERGNEM TEVLEEFPELEDPQTGEKLIDRTVLIANTSNMPVAAREASIYTGVTIAEY FRDQGLDVALMADSTSRWAEAMREVSARLEEMPGERGYPAYLASRLAEFY 50Nautilia_profundicola_AmH/1571/1-571 DFMFPIAKGGSASIPGGFGTGKTILQQTLAKYCDADIIIYIGCGERGNEM TEVLEEFPNLTDPYSGKPLMNRTVLIANTSDMPVSAREASIYLGITIGEY FRDQGYNVALMADSTSRWAEAMRELSSRMGELPMEEGYPADISSKIASVY 82Pyrobaculum_aerophilum_str._IM2/1593/1-593 DTMFPIAKGGTAAVPGPFGSGKTVMIRTLSMFAQSRFIIPVLCGERGNEA ADALQGLLKLKDPSTGRPLLERTTIIVNTSNMPVAAREASVYMGTTLGEY FRDQGYDVLVLADSTSRWAEAMREVALRIGEMPSEEGYPAYLPTRLAEFY 68Allochromatium_vinosum_DSM_180/1598/1-598 DTFFPQLKGGKGAVPGPFGAGKTVVQQQIARWSNADIVIYVGCGERGNEL VDVLETFPQLDDPYSGRKLMERTLLVANTSNMPVVAREASVYVGITMAEY YRDMGYDVVMLADSTSRWAEALREVSGRLGQMPVEEGYPAYLASRLAAVY 89Staphylothermus_marinus_F1/1592/1-592 DFFFPLAKGGKAAIPGGFGTGKTVTLQQLTKWSAVDIAIYVGCGERGNEM ADALHSFRRLVDPRTGKALVERSVFIANTSNMPVAARETSIFLGATIGEY FRDMGYHVLMVADSTSRWAEAMREISGRLEELPGEEGYPAYLGSRLASFY 184Clostridium_botulinum_A_str._ATCC_3502/1592/1-592 DTFFPVTKGGTACVPGPFGSGKTVVQHQLAKWADAQIVVYIGCGERGNEM TDVLNEFPELKDPKTGEPLMKRTVLIANTSNMPVAAREASIYTGITIGEY FRDMGYSIALMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRAAEFY 205Ferroglobus_placidus_DSM_10642/1573/1-573 DTLFPVAKGGTAAIPGPFGSGKTVTQHQLAKWSDTHVVVYIGCGERGNEM TEVLEEFPELEDPRTGKPLMERTVLIANTSNMPVAAREASVYTGITIAEY FRDMGYDVGLMADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAEFY 87Ignisphaera_aggregans_DSM_17230/1591/1-591 DYMFPIAKGGKAAIPGGFGTGKTVALQEITKWSHADIAIYVGCGERGNEM SDALTSFLKLMDTRRGRPMMERSVFIANTSNMPVAARETSVFLGVTIGEY FRDMGYDVVMVADSTSRWAEAMREISGRMEEMPGEEGFPAYLSSRLAEFY 206Archaeoglobus_fulgidus_DSM_4304/1581/1-581 DTFFPVAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQIVVYIGCGERGNEM TEVLEEFPELEDPRTGKPLMERTVLVANTSNMPVAAREASVYTGITIAEY FRDMGYDVAIQADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAEFY 108Coprothermobacter_proteolyticus_DSM_5265/1589/1-589 DVFFPVIKGGTACVPGPFGSGKTVIQHQLAKWSDVDVVVYVGCGERGNEM TEVLIEFPELKDPRTGGPLMDRTILVANTSNMPVAAREASVFTGIAIAEY YRDMGYDVALMADSTSRWAEAMREISTRLEEMPGEEGYPAYLASRISAFY 91Thermosphaera_aggregans_DSM_11486/1584/1-584 DFFFPIGKGGKAAIPGGFGTGKTVTLQGLTKRSEADITIYIGCGERGNEM ADALHSFIKLTDFRTGRPLMERSVFIANTSNMPVAAREASVFMGVTIGEY YRDMGYNVLLVADSTSRWAEAMREISGRLEELPGEEGFPAYLGSRLASFY 246Methanococcus_maripaludis_C6/1586/1-586 DTFFGLAKGGASAIPGPFGSGKTVTQHQLAKWSDVDVVVYIGCGERGNEM TEVIEEFPHLDDIKTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEY FRDQGLGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLSSKLAQFY 135Streptococcus_pneumoniae_CGSP14/1599/1-599 DTFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEM TDVLNEFPELIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEY FRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYY 125Dictyoglomus_turgidum_DSM_6724/1589/1-589 DTFFPLVKGGTACVPGPFGSGKTIIQHQLAKWADTEVVVYIGCGERGNEM TEVLIEFPELKDPRTGKPLMERTILIANTSNMPVAAREASVFTGITIAEY FRDMGYHVALMADSTSRWAEAMREISGRLGEMPGEEGYPAYLASRVASFY 11Halomonas_elongata_DSM_2581/1627/1-627 DTFFPIAKGGTACIPGPFGAGKTVLQNLISRYSDVDIVIIVACGERAGEV VETITEFPQLADPHTGGSLMDRTIIVCNTSSMPVAAREASIHTGTTLGEY YRQMGYDVLLIADSTSRWAQAMRETSGRLEEIPGEEAFPAYLESSIRKLY 159Clostridium_difficile_630/1592/1-592 DTFFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEM TDVLLEFPELKDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEY FRDMGYSVALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLGSRLAQFY 800Nanosalina/1586/1-586 DGLFPIAKGGTAAVPGGFGTGKTVTQQSLAKFSDADIIVYIGCGERGNEM TEVLEEFPELEDPQTGDKIINRTVLIANTSNMPVAAREASIYTGVTIAEY FRDMGLDVALMADSTSRWAEAMREISARLEEMPGERGYPAYLASRLAEFY 996Pyrococcus_sp._NA2/1588/1-588 DTFFPQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVVYIGCGERGNEM TDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKLAEFY 219Methanoculleus_marisnigri_JR1/1584/1-584 DGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEM TEVLTEFPELEDPKTGKPLMERTVLIANTSNMPVAAREASVYTGITIAEY FRDMGYDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 65Finegoldia_magna_ATCC_29328/1588/1-588 DVFFPLAKGGTVAIPGGFGTGKTMTQHQLAKYADADIIIYIGCGERGNEM TEVLEDFPKLIDPNNGKPLMERTVLIANTSNMPVAAREASIYTGITMAEY FRDMGYDVAIMADSTSRWAEALREISGRLEEMPAEEGYPAYLPSRIAQFY 38Borrelia_recurrentis_A1/1576/1-576 DTFFPVAKGGTFCIPGPFGAGKTVLQQVTSRNADVDIVIIAACGERAGEV VETLKEFPELTDPRTGKSLMERTCIICNTSSMPVAAREASVYTAITISEY YRQMGLDILLLADSTSRWAQAMREMSGRLEEIPGDEAFPAYLESVIASFY 32Desulfohalobium_retbaense_DSM_5692/1589/1-589 DTFFPIARGGTYCIPGPFGAGKTVIQQLTSRHADVDVVIVAACGERAGEV VETIREFPELEDPRTGRSLMERTIIICNTSSMPVAAREASVYTAVTLAEY YRQMGLNVLLLADSTSRWAQALRELSGRLEEIPGEEAFPAYLESVIAAFY 163Mahella_australiensis_501_BON/1587/1-587 DTLFPVAKGGTACIPGPFGSGKTVVQHQLAKWADADIIVYVGCGERGNEM TDVLMEFPELKDPRSGQPLMKRTVLIANTSDMPVAAREASIYTGITIAEY FRDMGYSVALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLSSRLAEFY 129Eubacterium_limosum_KIST612/1601/1-601 DTLFPIAKGGIAAIPGPFGSGKTVVQHQLAKWADADIIVYIGCGERGNEM TDVLMEFPELHDPRTGLSLMKRTVLIANTSDMPVAAREASIYTGITIAEY FRDMGYKVAIMADSTSRWAEALREMSGRLEEMPGEEGYPAYLSSRLAEFY 69Sideroxydans_lithotrophicus_ES1/1599/1-599 DTFFPLVKGGRAAVPGPFGAGKTIVQQQIARWSDADIVIYVGCGERGNEL VDILESFPKLTDPNTGRSLMERTLLVANTSNMPVVAREASIYVGATLAEY YRDQGYDVVMVADSTSRWAEALREVAGRLGQMPVEEGYPAYLASRLAAFY 214Methanocella_paludicola_SANAE/1579/1-579 DCLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEM TEVLSEFPHLTDPKSGNPLMDRTVLIANTSNMPVAAREASVYTGITIAEY YRDMGYGVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSQFY 70Nitrosococcus_halophilus_Nc4/1593/1-593 DTFFPLLKGSKAAVPGPFGAGKTIVQHQIARWSNADIVIYVGCGERGNEL VEVLDSFPQLTDPHTGRSLMERTLLVANTSNMPVVAREASLYVGVTLGEY YRDQGYDVVIVADSTSRWAEALREVAGRLGQMPVEEGYPAYLASRLAAFY 229Thermococcus_sibiricus_MM_739/1585/1-585 DTFFPQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAEVVVYIGCGERGNEM TDVLEEFPKLKDPRTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYNVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKVAEFY 175Clostridium_perfringens_SM101/1591/1-591 DTFFPVAKGGAAAVPGPFGAGKTVVQHQVAKWGDTEIVVYVGCGERGNEM TDVLNEFPELKDPKTGESLMKRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVSIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRLADYY 179Clostridium_tetani_E88/1592/1-592 DTFFPVTKGGTACVPGPFGSGKTVVQHQLAKWADAQIVVYIGCGERGNEM TDVLNEFPELKDPKTGESLMKRTVLIANTSNMPVAAREASIYTGITIGEY FRDMGYSIALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLGSRLAEFY 107Aeropyrum_pernix_K1/1597/1-597 DTFFPMAKGGTGAVPGGFGTGKTVTLHSLAQWSEARVVIYIGCGERGNEM TEVLERFPQYKDPWTGKPLMDRTVLIANTSNMPVAAREASIYVGITIAEY YRDMGYDVLLVADSTSRWAEALREIAGRLEEMPAEEGYPSYLASRLAEFY 105Ignicoccus_hospitalis_KIN4/I/1596/1-596 DTLFPMAKGGTGAIPGPFGSGKTVTLRTLAAWSDAKVVIYVGCGERGNEM TDVLVNFPHYKDPWSGRPLMERTILVANTSNMPVAAREASIYVGVTLAEY YRDMGYDSLLIADSTSRWAEALRDIAGRMEEMPAEEGFPPYLASRLAEYY 301Thetis_Sea/1-587/1-751 DTFFPVAKGGTASIPGGFGTGKTVTLHQLAKWSDTQIVVYVGCGERGNEM TDVLLHFPELEDPRTGEPLMDRTALVANTSNMPVAAREASIYTGITIAEY FRDMGYDIALMADSTSRWAEALREISGRLEEMPSEEGYPAYLGSRLAEFY 31Desulfarculus_baarsii_DSM_2075/1581/1-581 DSFYPVAKGGTACIPGPFGSGKTVLQQIISRYADVDVIIVAACGERAGEV VETLREFPHLEDPYTGRTLMERTVIICNTSSMPVAAREASIYTAITIGEY YRQMGLDVLLLADSTSRWAQALREMSGRLEEIPGEEAFPAYLQSRIAEFY 158Clostridium_phytofermentans_ISDg/1589/1-589 DTLFPIAKGGVATVPGPFGSGKTVVQHQLAKWAEADIVVYIGCGERGNEM TDVLNEFPELIDPKTGYSIMERTVLIANTSDMPVAAREASIYVGISIAEY YRDMGYSVALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLGSRLAQFY 193Halobacterium_salinarum_R1/1585/1-585 DGLFPIAKGGTAAIPGPFGSGKTVTQQSLAKFADADIVVYIGCGERGNEM TEVIEDFPELPDPQTGNPLMARTTLIANTSNMPVAARESCIYTGITIAEY YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 46Spirochaeta_thermophila_DSM_6192/1591/1-591 DTFFPVARGGTYCIPGPFGAGKTVLQQITSRNAEVDIVIIAACGERAGEV VETLREFPELIDPRTGRTLMERTVIVCNTSSMPVAAREASVYTAVTLAEY YRQMGLDCLLLADSTSRWAQAMREMSGRLEEIPGEEAFPAYLESVIASFY 196Halorhabdus_utahensis_DSM_12940/1587/1-587 DGLFPVAKGGTAAIPGPFGSGKTVTQHQLAKWADADIVVYVGCGERGNEM TEVIEDFPELEDPVTGKPLMSRTSLIANTSNMPVAARESSVYTGITMAEY YRDMGYDVALMADSTSRWAEALREISSRLEEMPGEEGYPAYLGARLSEFY 12Legionella_longbeachae_NSW150/1603/1-603 DTFFPIARGGTACIPGPFGAGKTVLQNLIARYSDVDIVIIVACGERAGEV VETITEFPNLVDPKMGGTLMDRTIIVCNTSSMPVAAREASIYTGITLGEY YRQMGFNVLLIADSTSRWAQAMRETSGRMEEIPGEEGFPAYLDSSIRGLY 236Methanocaldococcus_infernus_ME/1589/1-589 DTFFTLAKGGTAAIPGPFGSGKTVTQHQLAKWSDADVVVYIGCGERGNEM TEVIEEFPHLEDIRTGNKLMDRTVLIANTSNMPVAAREASVYTGVTIAEY FRDMGYGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFY 300Thermococcus_litoralis_DSM_5473]/1585/1-585 DTFFPQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAEVVVYIGCGERGNEM TDVLEEFPKLKDPRTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYNVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKIAEFY 231Pyrococcus_horikoshii_OT3/1964/1-588 DTFFPQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVIYIGCGERGNEM TDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKLAEFY 73Anaeromyxobacter_sp._Fw1095/1578/1-578 DCFFPVSAGGTAMVPGGFGTGKTVLEQSLAKHAAADVVVYVGCGERGNEM AEVLDELPKLEDPRSGGPLLGRTVLVVNTSNMPVAAREASIYTGCTIAEY FRDMGRSVALMIDSTSRWAEALREISARLEEMPGEEGYPTYLASRLARFY 194Halalkalicoccus_jeotgali_B3/1587/1-587 DGLFPLAKGGTAAIPGPFGSGKTVTQQSLAKFSDADIVVYIGCGERGNEM TEVIDDFPDLPDPQTGNALMARTCLIANTSNMPVAARESCVYTGITIAEF YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSQFY 202Haloterrigena_turkmenica_DSM_5511/1587/1-587 DGLFPLAKGGTAAIPGPFGSGKTVTQQQLAKWSDADIVVYIGCGERGNEM TEVIEDFPELPDPQTGNPLMARTCLIANTSNMPVAARESCIYTGITIAEY YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAAALSEFY 997Methanothermus_fervidus_DSM_2088/1585/1-585 DTFFPIAKGGTAAIPGPFGSGKTVTQQQLAKWADADIVIYVGCGERGNEM TEVLEEFPKLEDPRTGRPLMERTVLIANTSNMPVAAREACVYTGITIAEY FRDMGYDVALMADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFY 802Parvarchaeum/1583/1-583 DTMFPVAMGGTAAVPGPFGSGKTVIQHQLAKWSDADVIVYVGCGERGNEM TEILATFPELKDPKSGKPLMDRTILIANTSNMPVAAREASIYTGITLAEY YRDMGYSVAVMADSTSRWAEALRELSGRLEEMPGEEGYPAYLGRRVGEFY 208Methanosarcina_barkeri_str._Fusaro/1578/1-578 DGLFPVAKGGTAAIPGPFGSGKTVTQQSLAKWSDTEIVVYIGCGERGNEM ADVLSEFPELEDPQTGRPLMERTVLIANTSNMPVAAREASVYTGITIAEY FRDMGLDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLSARLAEFY Ferroplasma_sp._Type_II DTFFPVAKGGTVAVPGPFGSGKTVVQHQLSKWADSDIVVYVGCGERGNEM TEILTTFPELQDPKSGKPLMERTILIANTSNMPVAAREASIYTGISIAEY YRDMGYGIALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRLSEFY 84Caldivirga_maquilingensis_IC167/1595/1-595 DTVFPIAKGGAASIPGPFGSGKTVTIRSLMLYAMTQYSVPVLCGERGNEA ADALQGLLKLSDPATGRPLLERETIIVNTSNMPVAAREASIYMGATIAEY FRDQGYDVLLMADSTSRWAEAMREVALRIGEMPSEEGFPAYLPTRLAEFY 197Haloquadratum_walsbyi_DSM_16790/1585/1-585 DGLFPIAKGGTAAIPGPFGSGKTVTQHQLAKYADADIIIYVGCGERGNEM TEVIDDFPELEDPSTGNPLMARTSLIANTSNMPVAARESCVYTGITIAEF YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLAQFY 232Pyrococcus_abyssi_GE5/11017/1-588 DTFFPQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVIYIGCGERGNEM TDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKLAEFY 200Halomicrobium_mukohataei_DSM_12286/1586/1-586 DGLFPIAKGGTAAIPGPFGSGKTVTQHQLAKWADADIVVYVGCGERGNEM TEVIEDFPELEDPTTGNALMDRTCLIANTSNMPVAARESCVYTGITIAEY FRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 143Streptococcus_sanguinis_SK36/1623/1-623 DTFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEM TDVLNEFPELIDPTTGQSIMQRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYY 30Waddlia_chondrophila_WSU_861044/1596/1-596 DTQVPVVKGGTFCSPGPFGAGKTVLQHHLAKYSSVDIVVVVACGERAGEV VEMLREFPHLIDPHTGETLITRTVIICNTSSMPVAARESSIYMGMTVAEY YRQMGLDVLLLADSTSRWAQALREMSGRLEEIPGEEAFPAYLSSRIADFY 45Spirochaeta_smaragdinae_DSM_11293/1588/1-588 DTFFPVALGGTYCIPGPFGAGKTVLQQITSRHAEVDIVIIAACGERAGEV VETLKEFPELIDPRTGKSLMERTIIICNTSSMPVAAREASVYTAVTLAEY YRQMGLNVLMLPDSTSRWAQAMREMSGRLEEIPGEEAFPAYLESVIAGFY 131Thermanaerovibrio_acidaminovorans_DSM_6589/1569/1-569 DTLFPIARGGTACVPGPFGSGKTVIQHQLAKWADAEIVVYIGCGERGNEM TDVLLEFPELEDPRSGQPLMKRTVLIANTSNMPVAAREASIYTGITIAEY YRDMGYSVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGTRLASFY 42Treponema_pallidum_subsp._pallidum_str._Nichols/1589/1-589 DTFFPVAKGGTYCIPGPFGAGKTVLQHSTSRNADVDVVVIAACGERAGEV VETLREFPDLTDPRTGRSLMERTVIVCNTSSMPVASREASVYTGVTLAEY YRQMGLDVLLLADSTSRWAQALREMSGRLEEIPGEEAFPAYLESCIAAFY 77Thermofilum_pendens_Hrk_5/1601/1-601 DTFFPIAKGGAGAIPGGFGTGKTVTLHKVSMYSDSQIVVYIGCGERGNEI AEMLKEFPVLVDPKSGRPIIERSIIIANTSNMPVSAREASIYMGVTIAEY YRDQGYDVTLIADSTSRWAEALREIAGRLGELPVERGYPAYLPDKIAEFY 110Thermus_thermophilus_HB27/1578/1-578 DVLFPVAMGGTAAIPGPFGSGKTVTQQSLAKWSNADVVVYVGCGERGNEM TDVLVEFPELTDPKTGGPLMHRTVLIANTSNMPVAAREASIYVGVTIAEY FRDQGFSVALMADSTSRWAEALREISSRLEEMPAEEGYPPYLAARLAAFY 203Natrialba_magadii_ATCC_43099/1587/1-587 DGLFPIAKGGTAAIPGPFGSGKTVTQHQLAKWADADIVVYVGCGERGNEM TEVIEDFPELEDPKTGKPLMSRTCLIANTSNMPVAARESCIYTGITIAEY FRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAASLSEFY 93Sulfolobus_acidocaldarius_DSM_639/1592/1-592 DTIFPIAKGGTAAIPGPFGSGKTVTLQSLAKWSEAKVVIYVGCGERGNEM TDELRQFPKLKDPWTGKPLLQRTILVANTSNMPVAARESSIYVGVTMAEY FRDQGYDVLLVADSTSRWAEALRELGGRMEEMPAEEGFPSYLPSRLAEYY 35Borrelia_burgdorferi_B31/1575/1-575 DTFFPVAKGGTFCIPGPFGAGKTVLQQVTSRNADVDVVIIAACGERAGEV VETLKEFPELMDPKTGKSLMDRTCIICNTSSMPVAAREASVYTAITIGEY YRQMGLDILLLADSTSRWAQAMREMSGRLEEIPGEEAFPAYLESVIASFY 55Spirochaeta_smaragdinae_DSM_11293/1592/1-592 DTLFPLAKGGTVAIPGGFGTGKTMTQHAVAKWCDADIIIYIGCGERGNEM TDVLTEFPQLVDPRTGTSLMERTILIANTSNMPVSAREASVYTGVTLAEY FRDQGYHVAVMADSTSRWAEAMRELSGRMEEMPAEEGFPAYLPTRIAEFY 29Candidatus_Protochlamydia_amoebophila_UWE25/1593/1-593 DTQFPLMKGGTFCTPGPFGAGKTVLQHHLSKYAAVDIVLFVACGERAGEV VEVLREFPHLIDPHTDEALMKRTVIICNTSSMPVAARESSIYMGITIAEY YRQMGLDVLVLADSTSRWAQALREMSGRLEEIPGEEAFPAYLSSRIAEFY 198Haloferax_volcanii_DS2/1586/1-586 DGLFPIAKGGTAAIPGPFGSGKTVTQHQLAKWADADIVVYVGCGERGNEM TEVIEDFPELDDPKTGNPLMARTCLIANTSNMPVAARESCIYTGITIAEY YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 302Thermoplasmatales_archaeon/1-575 DTFFPVAKGGTVAVPGPFGSGKTVVQHQLAKWADSEVVIYVGCGERGNEM TEILTTFPELTDPKTGKPLMERTILIANTSNMPVAAREASIYTGVTMAEY FRDMGYNVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRVAEFY 62Halothermothrix_orenii_H_168/1590/1-590 DTFFPLGMGGTAAIPGGFGAGKTMTQHQLAKWSSADIIVYVGCGERGNEM TDVLEEFPKLEDPSTGKSMMERTVLIANTSNMPVAAREASIYTGITIAEF YRDMGYNVALMADSTSRWAEALREISGRLEEMPAEEGFPAYLPSRLAEFY 115Truepera_radiovictrix_DSM_17093/1579/1-579 DVLFPLTMGGTAAIPGPFGSGKTVTQQSIAKYGNADIVVYVGCGERGNEM TDVLVEFPELEDPQTGNPLMHRTVLIANTSNMPVAAREASIYTGITLAEY FRDQGYRVSVMADSTSRWAEALREIASRLEEMPAEEGYPPYLASRLAAFY 16Chlamydophila_pneumoniae_CWL029/1591/1-591 DTQIPVLKGGTFCTPGPFGAGKTVLQHHLSKYAAVDIVILCACGERAGEV VEVLQEFPHLIDPHTGKSLMHRTCIICNTSSMPVAARESSIYLGVTIAEY YRQMGLDILLLADSTSRWAQALREISGRLEEIPGEEAFPAYLSSRIAAFY 213Methanohalophilus_mahii_DSM_5219/1576/1-576 DGMFPVAKGGTAAIPGPFGSGKTVTQQQLAKWSDTDIVVYIGCGERGNEM ADVLNEFPELEDPKTGRPLMERTVLIANTSNMPVAAREASVYTGITIAEY YRDMGYDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLSARLSEFY 78Candidatus_Korarchaeum_cryptofilum_OPF8/1595/1-595 DTFFPIVKGGTSAVPGGFGTGKTVTLHQISKWADADVVVYVGCGERGNEM TDLLHTFPKLVDPKTGRPLLERSVLIANTSNMPVPAREASIFMGVTYAEY YRDMGLHVVLTADSTSRWAEALRELSSRLEEIPSEKGFPAYLPDFIASFY 19Chlamydia_muridarum_Nigg/1591/1-591 DTQIPVLKGGTFCTPGPFGAGKTVLQHHLSKYAAVDIVVLCACGERAGEV VEILQEFPHLTDPHTGQSLMHRTCIICNTSSMPVAARESSIYLGITIAEY YRQMGLHVLLLADSTSRWAQALREISGRLEEIPGEEAFPAYLASRIAAFY 52Thermanaerovibrio_acidaminovorans_DSM_6589/1594/1-594 DGLFPIAKGGTAAIPGGFGTGKTVTQHQLAKWSEAQVVVYIGCGERGNEM TDVLEEFPVLEDPRSGRPLMERTILIANTSNMPVAAREASIYTGITIAEY FRDMGYHVALMADSTSRWAEALREISGRLEEIPAEEGFPAYLPSRLAEFY 223Methanopyrus_kandleri_AV19/1592/1-592 DTFFPVAKGGTAAIPGPFGSGKTVTQQQLAKWADAQVVVYIGCGERGNEM TEVLEDFPELEDPRTGRPLMERTILVANTSNMPVAAREACIYTGITMAEY YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASRLAEFY 189Leptotrichia_buccalis_DSM_1135/1597/1-597 DTFFPVTKGGTACVPGPFGSGKTVVQHQMAKWADAEIIVYVGCGERGNEM TDVLMEFPEIIDPKTGQSLMKRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRAAEFY 191Fusobacterium_nucleatum_subsp._nucleatum_ATCC_25586/1382/1-584 DTFFAVTKGGTAAIPGPFGSGKTVIQHQLAKWADAEVVVYVGCGERGNEM TDVLMEFPEIIDPKTGQSLMKRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVALMADSTSRWAEALREMSGRLEEMPGDEGYPAYLASRIAEFY 4Bacteroides_vulgatus_ATCC_8482/1584/1-584 DTVNPIVEGGTGFIPGPFGTGKTVLQHAISKQAEADIVIIAACGERANEV VEIFTEFPELVDPHTGRKLMERTIIIANTSNMPVAAREASVYTAMTISEY YRAMGLKVLLMADSTSRWAQALREMSNRMEELPGPDAFPMDISAIISNFY 95Metallosphaera_sedula_DSM_5348/1591/1-591 DTIFPIAKGGTAAIPGPFGSGKTVTLQSLSKWSEAKIVIFVGCGERGNEM TDELRSFPKLKDPWTGRPLLERTVMVANTSNMPVAAREASIYVGVTLAEY FRDQGYDVLTVADSTSRWAEALRDLGGRMEEMPAEEGFPSYLSSRIAEYY 44Treponema_denticola_ATCC_35405/1589/1-589 DTFFPVAKGGTYCIPGPFGAGKTVLQHATSRNADVDIVIIAACGERAGEV VETLTEFPELKDPKTGRTLMERTIIICNTSSMPVAAREASVYTGVTLAEY YRQMGLDVLLLADSTSRWAQALREMSGRLEEIPGEEAFPAYLESYIAAFY 90Desulfurococcus_kamchatkensis_1221n/1584/1-584 DFLFPLAKGGKAAVPGGFGTGKTVLLHTVTKWSEADITIYIGCGERGNEM ADALNSFIKLIDPKTGKTMMERSIFIANTSNMPVAAREASIFLGITLGEY YRDMGYNILLVADSTSRWAEAMREISGRLEELPGEEGFPAYLASRLAQFY 57Thermotoga_sp._RQ2/1586/1-586 DIFFPISKGGTAAIPGGFGTGKTITQHQLAKWADADVVVYIGCGERGNEM TEVLEEFPHLQDPRTGKPLMDRTVLIANTSNMPVSAREASIYTGITIAEY FRDMGYHVALMADSTSRWAEALRELSGRLEEMPVEEGYPAYLASRIAQFY 803Pyrococcus_yayanosii/1587/1-587 DTFFPQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVVYIGCGERGNEM TDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKIAEFY 228Methanobrevibacter_smithii_ATCC_35061/1584/1-584 DTFFSVAKGGAAAIPGPFGSGKTVTQQQLAKWADADIVIYIGCGERGNEM TEVLTEFPYLDDPKTGNPLMDRTVLIANTSNMPVAAREACVYTGITIAEY YRDQGYDVALMADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFY 13Cyanothece_sp._PCC_8801/1607/1-607 DTFLPIARGGTGCIPGPFGAGKTVLQSMISRFSAVDIVIVVACGERAGEV VETITEFPKMKDPKTGGSLMDRTIIICNTSSMPVAAREASIYTGITLGEY YRQMGYDVLLIADSTSRWAQAMRETSGRLEEIPGEEAFPAYLDSSIKAVY 168Thermoanaerobacter_pseudethanolicus_ATCC_33223/1590/1-590 DTLFPVTKGGTACIPGPFGSGKTVVQHQLAKWADAEIVVYIGCGERGNEM TDVLLEFPELKDPKTEEPLMKRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLARRLAEFY 26Chlamydophila_felis_Fe/C56/1588/1-588 DTHIPVLKGGTFCTPGPFGAGKTVLQHHLSKYSSVDIVILCACGERAGEV VEVLQEFPHLTDPHTGKSLMHRTCIICNTSSMPVAARESSIYLGITIAEY YRQMGLHILLLADSTSRWAQALREISGRLEEIPGEEAFPAYLASRIAAFY 80Thermoproteus_neutrophilus_V24Sta/1594/1-594 DTMFPIAKGGAAAVPGPFGSGKTVTIRTLSMFAQSRFIIPVLCGERGNEA ADALHGLLKLKDPTTGRSLLERTTIIVNTSNMPVAAREASVYMGTTLGEY FRDQGYDVLVLADSTSRWAEAMREVALRIGEMPSEEGYPAYLPTRLAEFY 114Deinococcus_radiodurans_R1/1582/1-582 DVMFPLVMGGAAAIPGPFGSGKTVTQQSVAKYGNADIVVYVGCGERGNEM TDVLVEFPELEDPKTGGPLMHRTILIANTSNMPVAAREASVYTGVTLAEY FRDQGYSVSLMADSTSRWAEALREISSRLEEMPAEEGYPPYLGAKLAAFY 122Methanocella_paludicola_SANAE/1579/1-579 DLIFPVTKGGTAMIPGGFGTGKTVSEQTLAKWSDARIVVYIGCGERGNEM TDVLTEFPELVDPRTKLPLIQRTIMIANTSNMPVAAREASIYTGITMAEY FRDMGYDVALMADSTSRWGEALREVSGRLEEMPGEEGYPAYLPTRLAAFY 217Methanoplanus_petrolearius_DSM_11571/1582/1-582 DGLFPIAKGGTAAIPGPFGSGKTVTQQALAKWSDAEIVVYIGCGERGNEM TEVLTEFPELEDPKTGKPLMERTILIANTSNMPVAAREASVYTGITIAEY FRDMGYDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLSARLSEFY 998Thermoplasma_acidophilum_1221B2/1590/1-590 RCAFPVAEAANCRVPGPFGSGKTVIQHQLAKWSDANIVVYIGCGERGNEM TEILTTFPELKDPNTGQPLMTGLSFIANTSNMPVAAREASIYTGITIAEY YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRVSEFY 204Methanosaeta_thermophila_PT/1576/1-576 DGFFPLAKGGTAAIPGGFGTGKTVMQQTLSKWSDVDIVIYVGCGERGNEM ADLLHEFPELVDPRTNRPLLERSIVYANTSNMPVAAREASIYTGMTTAEY YRDMGYDVLMTADSTSRWAEAMRELASRLEEMPGEEGYPAYLAARLADFY 218Methanospirillum_hungatei_JF1/1582/1-582 DGLFPVAKGGTAAIPGPFGSGKTVTQQALAKWSDAEIVVYIGCGERGNEM TEVLTEFPELEDPKTGRPLMERTVLIANTSNMPVAAREASVYTGITIAEY FRDMGYDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 221Candidatus_Methanosphaerula_palustris_E19c/1588/1-588 DGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAEIVVYIGCGERGNEM TEVLTEFPELEDPKTGKPLMERTILIANTSNMPVAAREASVYTGITIAEY FRDMGYDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 171Anaerococcus_prevotii_DSM_20548/1585/1-585 DTFFPVAKGGTAAIPGPFGSGKTVVQHQIAKFADADIVIYVGCGERGNEM TDVLNEFPELIDPKTGESIMKRTVLIANTSNMPVAAREASIYTGVTLAEY FRDMGYNVAMMADSTSRWAEALREMSGRLEEMPGDEGFPAYLASRIADFY 120Ferroplasma_acidarmanus_fer1/1589/1-589 DSFFPVAKGGTVAVPGPFGSGKTVIQHQLSKWSDADITVYVGCGERGNEM TEILSTFPELQDPKSGKPLMEKTILIANTSNMPVAAREASIYTGVTIAEY YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRISEFY 119Thermoplasma_volcanium_GSS1/1776/1-590 DALFPVAKGGTAAVPGPFGSGKTVIQHQLAKWSDANIVVYIGCGERGNEM TEILTTFPELKDPVSGQPLMDRTVLIANTSNMPVAAREASIYTGITIAEY YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRISEFY 3Brachyspira_murdochii_DSM_12563/1587/1-587 DTFNPITEGGTGFIPGPFGAGKTVLQHALATNANADLIIMTACGERANEV VEIFTEFPELIDPRTGRSLMERTTIICNTSNMPVAAREASVYVGMTIAEY YRSMGLKVLLLADSTSRWAQALREMSNRLEELPGPDAFPIDLPAIISSFY 59Anaerococcus_prevotii_DSM_20548/1582/1-582 DVFFPLAKGGTVAIPGGFGTGKTMLQHQLAQYSDVDIIIYIGCGERGNEM TQVLEEFPDLIDPNTGKGLMERTILIANTSNMPVAAREASIYTGITMAEY FRDMGYDVALMADSSSRWAEALREISGRLEEIPAEEGYPAYLGSRLSQFY 164Clostridium_thermocellum_ATCC_27405/1589/1-589 DTLFPLAKGGVAAVPGPFGSGKTVVQHQLAKWADADIVVYIGCGERGNEM TDVLKEFPELKDPKTGESLMKRTVLIANTSDMPVAAREASIYTGMTIAEY FRDMGYSVALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLGSRLAQFY 237Methanocaldococcus_vulcanius_M7/1587/1-587 DTFFTLAKGGTAAIPGPFGSGKTVTQHQLAKWSDADVVVYIGCGERGNEM TEVIEEFPHLEDIRTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEY FRDMGYGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFY 124Dictyoglomus_thermophilum_H612/1589/1-589 DTFFPLVKGGTACVPGPFGSGKTIIQHQLAKWADAEVVVYIGCGERGNEM TEVLIEFPELKDPRTGKPLMERTVLIANTSNMPVAAREASVFTGITIAEY FRDMGYHVALMADSTSRWAEAMREISGRLGEMPGEEGYPAYLASRVASFY 153Streptococcus_pyogenes_M1_GAS/1591/1-591 DTFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEM TDVLNEFPELIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVAIMADSTSRWAEALREMSGRLQEMPGDEGYPAYLGSRIAEYY 41Fibrobacter_succinogenes_subsp._succinogenes_S85/1585/1-585 DTFFPVMQGGTFCTPGPFGAGKTVLQQLMSRYADVDIVILAACGERAGEV VETLREFPELIDPRTGKSLMERTLIICNTSSMPVAAREASVYTGVTLAEY YRQMGLNVLLLADSTSRWAQALREMSGRLEEIPGEEAFPAYLESVIASFY 220Candidatus_Methanoregula_boonei_6A8/1588/1-588 DGLFPIAKGGTAAIPGPFGSGKTVTQQQLAKWSDAKIVVYIGCGERGNEM TEVLTEFPHLEDPTSGKPLMERTVLIANTSNMPVAAREASVYTGITIAEY FRDMGYDVSLMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 79Pyrobaculum_islandicum_DSM_4184/1593/1-593 DTMFPIAKGGTAAIPGPFGSGKTVAIRTLSMYAQSRIIIPVLCGERGNEA ADALQGLLKLKDPNTGRPLLERTTIIVNTSNMPVAAREASVYMGTTIGEY FRDQGYDVLVLADSTSRWAEAMREVALRIGEMPSEEGYPAYLPTRLAEFY 177Clostridium_botulinum_E3_str._Alaska_E43/1592/1-592 DTFFPVAKGGAAAIPGPFGAGKTVTQHQIAKWGDAEIVVYVGCGERGNEM TDVVNEFPELIDPKTGESLMKRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYAVSIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRLADYY 48Geobacter_uraniireducens_Rf4/1524/1-524 DFLFPLARGGAAVFPGGFGTGKTVLEQSVAKFSAVDLVVYVGCGERGNEM AELLDEFAALSDPWTGKPLMDRTIVVVNTSNMPVAAREASIYTAVTMAEY YRDMGYHVLLLADSISRWAEALREISSSLEEMPGEEGYPTYLASRLSGFF 211Methanohalobium_evestigatum_Z7303/1578/1-578 DGMFPVAKGGTAAIPGPFGSGKTVTQQQLAKWSDTDIVVYVGCGERGNEM ADVLDEFPELEDPKAGRPLMERTVLIANTSNMPVAAREASVYTGITISEY YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 230Pyrococcus_furiosus_DSM_3638/11013/1-588 DTFFPQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVVYIGCGERGNEM TDVLEEFPKLKDPNTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASRLAEFY 47Stackebrandtia_nassauensis_DSM_44728/1575/1-575 DLLFPVPRGGSAAVPGGFGTGKTVLLQQVAKWCDADVIVYIGCGERGNEM AEVVAELLSLTDPRSGGRLADRTVVIANTSNMPMMAREASIHTGMTVAEY FRDMGLHVVLIADSTSRWAEALREFASRNRELPAEEGYPAGLASALAAFY 199Haloarcula_marismortui_ATCC_43049/1586/1-586 DGLFPIAKGGTAAIPGPFGSGKTVTQHQLAKWADADIVVYVGCGERGNEM TEVIEDFPELEDPTTGNALMDRTCLIANTSNMPVAARESCVYTGITIAEY FRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLSARLSEFY 227Methanobrevibacter_ruminantium_M1/1584/1-584 DTFFCLAKGGAAAIPGPFGSGKTVTQQQLAKWADADIVVYIGCGERGNEM TEVLTEFPFLTDPKTGNPIMDRTVLIANTSNMPVAAREACVYTGITIAEY FRDQGYDVALMADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFY 10Parabacteroides_distasonis_ATCC_8503/1585/1-585 DTVNPIVEGGTGFIPGPFGTGKTVLQHAISKQAEADIVIIAACGERANEV VEVFTEFPELVDPHTGRKLMERTIIIANTSNMPVAAREASVYTAMTIAEY YRSMGLKVLLMADSTSRWAQALREMSNRLEELPGPDAFPMDLSAIVANFY 195Halorubrum_lacusprofundi_ATCC_49239/1591/1-591 DGLFPIAKGGTAAIPGPFGSGKTVTQHQLAKYADADIIVYVGCGERGNEM TEVIEDFPELEDPANGNPLMARTSLIANTSNMPVAARESCIYTGITIAEY YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLAQFY 64Thermosediminibacter_oceani_DSM_16646/1587/1-587 DTFFPLAKGGTAAIPGGFGTGKTITQHQLAKWSDADIIVYIGCGERGNEM TEVLEDFPKLQDPRTGKPLMDRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYNVALMADSTSRWAEALREIAGRLEEMPAEEGYPAYLASRLAQFY 144Streptococcus_gallolyticus_UCN34/1591/1-591 DTFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEM TDVLNEFPELIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYY 165Thermosediminibacter_oceani_DSM_16646/1590/1-590 DTLFPVTKGGTACIPGPFGSGKTVVQHQLAKWADAEIVVYIGCGERGNEM TDVLLEFPELKDPKTGEPLMKRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLARRLAEFY 172Clostridium_cellulovorans_743B/1591/1-591 DTFFPVAKGGAAAVPGPFGAGKTVVQHQIAKWGDAEIVVYVGCGERGNEM TDVLNEFPELIDPNTGKSIMNRTVLIANTSNMPVAAREASIYTGITIAEY YRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRLAEYY 201Natronomonas_pharaonis_DSM_2160/1585/1-585 DGLFPIAKGGTAAIPGPFGSGKTVTQHQLAKWADADIVVYVGCGERGNEM TEVIEDFPELEDPKTGKPLMSRTCLIANTSNMPVAARESCVYTGITIAEY YRDMGYDVALMADSTSRWAEAMREISSRLEEMPGEEGYPAYLAARLSEFY 178Clostridium_novyi_NT/1591/1-591 DTFFPVAKGGAAAVPGPFGSGKTVVQHQLAKWGDAQIVVYIGCGERGNEM TDVLNEFPELKDPKTGKSIMERTVLIANTSNMPVAAREACIYTGITIAEY FRDMGYSVALMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRLADFY 141Streptococcus_mitis_B6/1591/1-591 DTFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEM TDVLNEFPELIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEY FRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYY 109Methanospirillum_hungatei_JF1/1588/1-588 DTLFPLARGGTAAIPGPFGSGKTVVQQQLAKWVNADIIIYIGCGERGNEM ADVLEQFPTLKDPRTGHALSSRMVLIANTSNMPVAAREASVYTGITIAEY YRDMGYHVALMADSTSRWAEAMREISGRLEEMPGEEGYPAYLSSRLADFY 49Aminobacterium_colombiense_DSM_12261/1588/1-588 DTLFPIAIGGAAVLPGGFGTGKTVTQQSLAKWCNAEIIIYIGCGERGNEM TEVLEEFPELSDPFHNAPLMERMVLIANTSNMPVAAREASVYLGMTLAEY FRDMGYNTAIMADSTSRWAEALREIGGRLEEMPGEEGYPAYLGTRLAQYY 118Thermoplasma_acidophilum_DSM_1728/1764/1-590 DALFPVAKGGTAAVPGPFGSGKTVIQHQLAKWSDANIVVYIGCGERGNEM TEILTTFPELKDPNTGQPLMDRTVLIANTSNMPVAAREASIYTGITIAEY YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRVSEFY 94Sulfolobus_tokodaii_str._7/1595/1-595 DTVFPIAKGGTAAIPGPFGSGKTVTLQSLAKWSAAKVVIYVGCGERGNEM TDELRSFPKLKDPWTGKPLLLRTILVANTSNMPVAARESSIYVGVTMAEY FRDQGYDVLLVADSTSRWAEALRDLGGRMEEMPAEEGFPSYLPSRLAEYY 102Sulfolobus_islandicus_M.16.27/1592/1-592 DTIFPIAKGGTAAIPGPFGSGKTVTLQSLAKWSAAKIVIYVGCGERGNEM TDELRQFPSLKDPWTGRPLLERTILVANTSNMPVAAREASIYVGITMAEY FRDQGYDTLLVADSTSRWAEALRDLGGRMEEMPAEEGFPSYLPSRLAEYY 53Treponema_pallidum_subsp._pallidum_str._Nichols/1605/1-605 DVFFPLSKGGTAAIPGGFGTGKTMTQHAVAKWCDADIIVYIGCGERGNEM TDVLSEFPKLIDPRTGRSLMERTILIANTSNMPVSAREVSLYSGITLAEY YRDMGMHVAIMADSTSRWAEALRELSGRMEEMPAEEGFPAYLPTRLAEFY 106Hyperthermus_butylicus_DSM_5456/1601/1-601 DTMFPMAKGGTGAIPGPFGSGKTVTLQSLAKWSAAKVVIYIGCGERGNEM TEVLETFPKLTDPWTGKPMMERTILIANTSNMPVAAREASIYTGITLAEY YRDMGYDVLLVADSTSRWAEALREIAGRLEEMPAEEGYPSYLASRLAEFY 104Acidilobus_saccharovorans_34515/1601/1-601 DLLFPMAKGGTGMIPGGFGTGKTVTLHNLAQWSSANVIIYIGCGERGNEM TEVLDKFPTYKDPWTGRPLMERSILVANTSNMPVAAREASIYVGITMGEY YRDMGYDVLLVADSTSRWAEALREMAGRLEEMPAEEGYPSYLASRLAEFY 58Acholeplasma_laidlawii_PG8A/1583/1-583 DTLFPIAKGGTAAIPGGFGTGKTMLQHQLAKWSDADIIVYVGCGERGNEM TQVLEEFTKLVDPRTNQTLMDRTILIANTSNMPVAAREASIYTGLTVAEY YRDMGYHVAIMADSTSRWAEALREISARLEEIPAEEGYPSYLPSRISKFY 130Aminobacterium_colombiense_DSM_12261/1598/1-598 DAFFPIAKGGTACVPGPFGSGKTVIQHQLAKWAESDIVVYVGCGERGNEM TDVLLEFPELEDPRSGEPLMKRTVLIANTSNMPVAAREASIYTAITMAEY YRDMGYSVALMADSTSRWAEALREMSGRLEEMPGEEGYPAYLGTRLASFY 243Methanococcus_vannielii_SB/1586/1-586 DTFFGLAKGGASAIPGPFGSGKTVTQHQLAKWSDVDVVVYIGCGERGNEM TEVIEEFPHLDDIKTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEY FRDQGLGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLSSKLAQFY 207Archaeoglobus_profundus_DSM_5631/1580/1-580 DTFFPVAKGGTAAIPGPFGSGKTVTQHQLAKWADAQVIVYIGCGERGNEM TEVLEEFPELEDPRTGKPLMERTILIANTSNMPVAAREASVYTGITIAEY FRDMGYDVAIQADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRIAEFY 999Ferroplasma_acidarmanus/1589/1-589 DSLFPVAKGGTVAVPGPFGSGKTVIQHQLSKWSDADITVYVGCGERGNEM TEILSTFPELQDPKSGKPLMEKTILIANTSNMPVAAREASIYTGVTIAEY YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLGRRISEFY 116Meiothermus_ruber_DSM_1279/1578/1-578 DVLFPLAAGGTAAIPGPFGSGKTVTQQSIAKYGDANIVVYVGCGERGNEM TDVLVEFPELEDPQTGRPLMERTILVANTSNMPVAAREASLYTGITLAEY FRDQGYKVSLMADSTSRWAEALREIASRLEEMPAEEGYPPYLASRLSSFY 123Methanospirillum_hungatei_JF1/1584/1-584 DTMFPLVKGGTAMIPGGFGTGKTVSEQTLAKWADTQVVVYIGCGERGNEM TDVLTEFPELTDPRTGYPLIERTIMIANTSNMPVAAREASIYTGITIAEY YRDMGMDVALLADSTSRWGEALREVSGRLEEMPGEEGYPAYLATRLAAFY 226Methanothermobacter_thermautotrophicus_str._Delta_H/1584/1-584 DTFFSVAKGGTAAIPGPFGSGKTVTQQQLAKWADADIIVYVGCGERGNEM TEVLKEFPELEDPKTGNPLMDRTVLIANTSNMPVAAREACVYTGITIAEY FRDMGYDVALMADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFY 241Methanococcus_aeolicus_Nankai3/1586/1-586 DTFFGLAKGGASAIPGPFGSGKTVTQHQLAKWSDADIVVYIGCGERGNEM TEVIEEFPHLDDLKTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEY FRDMGYGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLSSRLAQFY 234Thermococcus_gammatolerans_EJ3/1585/1-585 DTFFSIAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVVYIGCGERGNEM TDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDQGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKIAEFY 224Methanosphaera_stadtmanae_DSM_3091/1585/1-585 DTFFCVAKGGTSAMPGPFGSGKTVTQQQLAKWADADIVVYIGCGERGNEM TEVLTEFPELEDPKTGNPLMDRTVLIANTSNMPVAAREACVYTGITIAEY FRDMGYDVALMADSTSRWAEAMRELSGRLEEMPGEEGYPAYLASRLAQFY 233Thermococcus_onnurineus_NA1/1585/1-585 DTFFSQAKGGTAAIPGPFGSGKTVTQHQLAKWSDAQVVVYIGCGERGNEM TDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASKIAEFY 66Eubacterium_limosum_KIST612/1595/1-595 DFFFPIAKGGTAAIPGGFGTGKTTTQHQLAKWSDADIIIYIGCGERGNEM TEVIEDFPGLIDPKSGESIMERTVMIANTSNMPVAAREASIYTGITMAEY FRDMGYDVAMMADSTSRWAEALREISGRLEEMPAEEGYPAYLSSRIAEFY 188Streptobacillus_moniliformis_DSM_12112/1588/1-588 DTFFPVSKGGTACVPGPFGSGKTVVQHQFAKWGDAQVVVYVGCGERGNEM TDVLMEFPEIIDPLTGKSLMQRTVLIANTSNMPVAAREASIYTGITIAEY FRDMGYSVSIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLSSRAADFY 140Streptococcus_pneumoniae_TIGR4/1591/1-591 DAFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEM TDVLNEFPELIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEY FRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGDEGYPAYLGSRIAEYY 60Clostridium_phytofermentans_ISDg/1588/1-588 DSLFPLAKGGTAAIPGGFGTGKTMTQHQLAKWSDADIIVYIGCGERGNEM TEVLEDFSKLVDPKSGNLLLDRTVLIANTSNMPVAAREASIYTGITLAEY YRDMGYHVAIMADSTSRWAEALRELSGRLEEMPAEEGFPAYLASRLSSFY 451 4 25Chlamydia_trachomatis_B/TZ1A828/OT/1591/1-591 ERGGAVKMKDGSE------GSLTICGAVSPAGGNFEEPVTQATLSVVGAF CGLSKARADARRYPSIDPMISWSKYLD--SVAEILEKKV-PGWGESVKQA SRFLEEGAEIGKRIEVVGEEGISMEDMEIFLKSELYDFCYLQQNAFDAED 801Micrarchaeum/1586/1-586 ERAGRVHILAGGV------GSVTAIGAVSPPGGDISEPVSQNTLRVTRVF WALDAALANSKHFPSINWLNSYSLYFE--DMKKWYVENVSKDWPELYLRA MQTLQKEAEINEIVQLVGYDALPEADKLTLDVAKSIREDYLQQSAFDDVD 222Aciduliprofundum_boonei_T469/1582/1-582 ERAGYVEVAGSEK----KYGSVTVVGAVSPPGGDFSEPVTQNTLRIVKVF WALDAELAHKRHFPSINWLRSYSLYLN--SVSSWWSKNVAKDWYELRREA MGILQREAELQEIVQLVGPDALPAKEQALLETARSLREDFLQQNAFHDVD 127Acholeplasma_laidlawii_PG8A/1592/1-592 ERAGLVETLGSKA----RTGAITAIGAVSPPGGDTSEPVSQATLRIVKVF WGLSASLAYRRHFPAIDWLKSYSLYDE--QIRDFFNTNVDKNWTHHVSFV STLLQEEASLQEIVRLVGVDSLGASDRLKLLVAQMIREDYLHQNAFDPID 85Vulcanisaeta_distributa_DSM_14429/1603/1-603 ERAGRVVTLGKGN----RLGSVTIAASVSPPGGDFTEPVTSHTLRFIGAF WPLDAKLAYSRHYPAINWLMGFSRYVD--TVAEWYQKNVAPDWRELRDEA VRILTREAELQEVVRILGTEALSEQEKHLLNAAFLIREGFLKQDAYHPVD 210Methanosarcina_mazei_Go1/1578/1-578 ERAGVAETLCGEK------GSITAIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQKRHFPAINWLNSYSLYKE--DLNDWFTENVAPDYVPMRERA MDMLQTESELQEIVQLVGSDALPEEQQLLLEITRMIREIFLQQNAFHPID 8Porphyromonas_gingivalis_ATCC_33277/1584/1-584 ARAGYVYLNNGSA------GSVTFIGTVSPAGGNLKEPVTENTKKVARCF YALEQNRADRKRYPAVNPIDSYSKYIEYPEFESYISNHISADWTTKVNEL KMRLHQGKEIAEQINILGDDGVPVSYHVVFWKAELIDFVILQQDAFDDVD 216Methanocorpusculum_labreanum_Z/1581/1-581 ERAGLVEPLAGGS------GSVSVIGAVSPAGGDFSEPVTQNTLRIVKVF WALDANLSRRRHFPAINWLQSYSLYMA--QLNDYYDEKVSPDWNKLRSWF MEVLQKEAELQEIVQLVGSDALPETEQITIEVARMMRELFLQQNGFDPVD 212Methanococcoides_burtonii_DSM_6242/1578/1-578 ERAGAVNSLAGLD------GSITVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQRRHFPSINWLTSYSLYTQ--GLADWYSENVGADWTQLRDDA MDLLQQESELQEIVQLVGSDALPEDQQLTLEVARMVREYFLQQNAFHPVD 242Methanococcus_voltae_A3/1585/1-585 ERAGRVDCLGSDD----RKGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVF WALDANLARRRHFPAINWLTSYSLYVD--DIAGWWKENTGADWRALRDEA MNLLQKEAELQEIVQLVGPDALPERERVILEIARMLREDFLQQDAFHEID 132Enterococcus_faecalis_V583/1595/1-595 ERAGQVIALGKDH----REGSITAISAVSPSGGDISEPVTQNTLRVVKVF WGLDSQLAQKRHFPSINWLQSYSLYST--EVGQYLDLELQGNWAAMVAEG MRILQEESQLEEIVRLVGIDSLSDKDRLTLETAKSLREDYLQQNAFDDVD 63Clostridium_sticklandii_DSM_519/1594/1-594 ERAGFVRSLSGVE------GSVTIIGAVSPPGGDFSEPVTENTKRFVNAF LALDRKLAYARHYPAIGYLTSYSGYSK--SLRNWYYENVSEDVLEIREKM LFILHEEEKLNSIVQLVGEDVLPDDQRLTLEIAKVIKRGFLQQNAMHKDD 215uncultured_methanogenic_archaeon_RCI/1579/1-579 ERAGRVITPIGKE------GSVTVIGAVSPAGGDISEPVTQNTLRIVKVF WALDAKLAQRRHFPSINWLNSYSLYQD--SLKDWYDKNISPEWNQLKAES MELLQRESELQEIVQLVGSDALPEDQQLTIEIARMIREIFLQQNAYHEVD 121Picrophilus_torridus_DSM_9790/1922/1-589 ERSGNAQIIAEDQ----RTGSVTLIGAVSPPGGDLSDPVVQNTLRVTRVF WALDASLASRRHFPSINWLTSYSLYTN--NLSKWYTENVGPDWPEIYKTM MDLLEKESELQEIVQLVGYDALPEKEKNVLDIAKMIREDFLQQNAFDDID 209Methanosarcina_acetivorans_C2A/1578/1-578 ERAGVAETLCGEK------GSITAIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQRRHFPAINWLNSYSLYKE--DLNDWFTENVAPDYVAMREKA MDMLQTESELQEIVQLVGSDALPEEQQLLLEITRMIREIYLQQNAFHPID 128Acidaminococcus_fermentans_DSM_20731/1590/1-590 ERAGEVECLGQED----RVGAVSAIGAVSPPGGDISEPVSQATLRIVKVF WCLSSALAYARHFPAIDWLQSYSLYSD--RLRAYYDAKVAPDWSKLVGTA MEILQEESNLNEIVRLVGIDALGFKDRITMECARSIREDFLHQNSFHEVD 5Bacteroides_fragilis_NCTC_9343/1585/1-585 GRAGYVKLNNGES------GSITFIGTVSPAGGNLKEPVTENTKKVARCF YALEQDRADKKRYPAVNPIDSYSKYIEYPEFEAYIAEHINDEWIGKVNEI KTRLQRGKEIAEQINILGDDGVPVEYHVIFWKSELIDFVILQQDAFDEID 96Sulfolobus_solfataricus_P2/1592/1-592 ERAGRVKTIGNPE----RFGSVTVASAVSPPGGDFTEPVTSQTLRFVKVF WPLDVSLAQARHYPAINWLQGFSAYVD--LVANWWNTNVDPKWREMRDMM VRTLIREDELRQIVRLVGPESLAEKDKLILETARLIKEAFLKQNAYDDID 75Anaeromyxobacter_dehalogenans_2CP1/1579/1-579 ERAGRVETLGGAE------GAVTMVGAVSPPGGDLSEPVTQCSLRATGAL WALSADLAHRRHYPAVDWSVSFTLEGD--RLAGWFEREAGDGFGALRDEA RKLLQRERELAEVAELVGTESLQDAERLVLESARLLREGFLRQSALDPAD 235Thermococcus_kodakarensis_KOD1/1585/1-585 ERAGRVITLGSDE----RVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLRSYSLYID--AIQDWWHKNVDPEWRKMRDTA MALLQKEAELQEIVRIVGPDALPDREKAILIVTRMLREDYLQQDAFDEVD 92Nitrosopumilus_maritimus_SCM1/1592/1-592 ERAGRVRAAGSPD----RDGSVTLIGAVSPSGGDFTEPVTTHTMRFIKTF WALDAKLAYSRHYPSINWMNSYSGYLA--DIAKWWGENINEDWLSLRSEV YGVLQREDTLKEIVRLLGPEALPDEEKLILEVARMVKIGLLQQNSFDDVD 190Finegoldia_magna_ATCC_29328/1587/1-587 ERAGKVKCLGSDG----RDGSLTVIGAVSPPGGDISEPVTQSTLRIVKVF WGLDYALSYMRHFPAINWINSYSLYQD--KIDEYLDANVDSKFSDYRKQS MKLLQEESSLLEIVRLVGRDSLSDEDQIKLETSKSLREDFLQQNAFHETD 999Nanosalinarum_sp._J07AB56/1584/1-584 ERGGRVRPLGPEE-----PGSVSIIGAVSPPGGDFSEPVTQNTLRVVKNF FALDKDLAEKRHFPSINWNDSYSGYSE--TLSDYWQEEVDEDWNQNVQRL RDLLQESDDLEETVQLVGKDALPDRDRLTLEIGDMLKEFYLQQNAFHPVD 50Nautilia_profundicola_AmH/1571/1-571 ERASIVETFASNI------GSLSIIGAVSPAGGDFSEPVTIHTKRFTSVF WALDKSLANARFYPAINYLNSYSNY----ELNEWWEEK--GPYIEIKQFF NDILQKDASLQKIVKLLGVGALPEEEKITLYTAELIKEAFLQQNAFDETD 82Pyrobaculum_aerophilum_str._IM2/1593/1-593 ERAGRVVLYGSKE----RVGSLTIAASVSPPGGDFTEPVTSNTLRFIGAF WPLSPRLAYSRHYPAIDWLVAFSRYVD--TVEVWWSKNVSPEWRRIRDAL QSILVKEAELQEIVRILGTEALSEYEKHILNVAFMIREGFLKQDAYNPVD 68Allochromatium_vinosum_DSM_180/1598/1-598 ERAGRVETFGAGS------GSVTLIGAVSPPGGDFSEPVTSHTKDIIETF WALSKELADARHYPSIDWVTSFSGHVK--TAAEWWHKEIDPDWERRRGAA LALLARDAELSRIVNLVGPEALSDAQRWELEGASLIKEGVLQQSALDEID 89Staphylothermus_marinus_F1/1592/1-592 ERSGYVKTLGRPD----RTGSLTIMGAVSPPGADFSEPVTQATLRIVRAL YALDVNLAYRRHYPAINWLISYSLYVD--NVTDWWHKNIDPSWRELREKA LAILQKEAELEELVRLVGAEALPEEDKLLLEVARMIREDFLQQNAFHEID 184Clostridium_botulinum_A_str._ATCC_3502/1592/1-592 ERAGNVVSIGSEE----REGALTVIGAVSPPGGDLSEPVTQATLRIVKVF WGLDAQLAYRRHFPAINWLNSYSLYIE--KISPWMDENVASDWTALRIKA MSLLQEEASLEEIVRLVGIDALSEKDRLKLEVAKSLREDYLQQNAFHEVD 205Ferroglobus_placidus_DSM_10642/1573/1-573 ERAGRVRTLNGNI------GSVTIIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLAARRHFPAINWLQSYSLYAD--TLAEWFNKNVAEDWSELRRWA MEVLQEEANLQEIVQLVGSDALPESQRILLEVARLIREVYLQQYAYHPVD 87Ignisphaera_aggregans_DSM_17230/1591/1-591 ERAGRVEALGRPQ----RYGSLTIFGAVSPPGGDFAEPVVRATVRYVQSF YALDYGLATRRHFPAINWLTSYSMYVP--YVAKWWDSLTNGEWSIFRGEA IRILQREAELSDIVRLVGPEALPEEDKLILEIARMIREDFLQQSAYVPAD 206Archaeoglobus_fulgidus_DSM_4304/1581/1-581 ERAGRVKTLAGNI------GSVTVVGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLAARRHFPAINWLQSYSLYVD--TLKDWFAENVSEEWNELRRWA MEVLQEEANLQEIVQLVGSDALPESQRVLLEVARIIREVYLIQYAYHPVD 108Coprothermobacter_proteolyticus_DSM_5265/1589/1-589 ERSGFVKCLGSPE----RTGSVTVIGAVSPPGGDLSDPVVQATLRSVQVF WGLDQDLAFARHFPAINWLNSYSLYLD--RIGEYYNKSFAPDWLKKRNEA MVILQRESELLELVRLVGYDALSTEEQLFLETARSLREDFLQQDAFDDVD 91Thermosphaera_aggregans_DSM_11486/1584/1-584 ERSGLVKTIGEPP----RVGSLTIIGAVSPPGADFSEPVTQATLRVVRAL YALDVNLAYRRHYPAINWLTSYSLYVE--HVKEWWSEQ-GGDYGLLREKL MSILQKEAELEELVRLVGTEALPAEDKLTLEVARMVREDFLQQDAFSPVD 246Methanococcus_maripaludis_C6/1586/1-586 ERAGRVECLGSEN----KQGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVF WALDANLARRRHFPAINWLTSYSLYID--DIAGWWQQNTAADWRSLRDEA MSLLQKEAELQEIVQLVGPDALPDRERVILEIARMLREDFLQQDAYHEVD 135Streptococcus_pneumoniae_CGSP14/1599/1-599 ERAGRSQVLGIPE----REGTITAIGAVSPPGGDISEPVTQNTLRIVKVF WGLDAPLAQRRHFPAINWLTSYSLYKD--SVGIYIDGKEKTDWNSKITRA MNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFDSVD 125Dictyoglomus_turgidum_DSM_6724/1589/1-589 ERAGLVKCLGKEN----KIGSVSIIGAVSPPGGDLSDPVVQATLRVTKVF WGLDARLAFSRHFPAVDWLVSYSLYLE--PVSRYWKENVAEDFYDLRQEA MAILQREAELQDIVRLVGMEALSPQDRLTLETARSIREDFLHQNALHEVD 11Halomonas_elongata_DSM_2581/1627/1-627 ERAGILDMHGGQC------GSLTLIGTVSPAGGNFEEPVTQGTLNTVKTF LGLSYDRAYKRFYPAVDPLISWSRYPE--QLSGWFRRTMGEHWQADVDRL KALLHEGDEIERMMQVTGEEGISDADFLSQQKATLVDMVYLQQDAFDKVD 159Clostridium_difficile_630/1592/1-592 ERAGKVNCLGMDE----REGTLTAIGAVSPPGGDTSEPVSQATLRIVKVF WGLDASLAYKRHFPAINWLTSYSLYQE--KVDVWADKNVNDNFSAQRAQA MKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHEVD 800Nanosalina/1586/1-586 ERAGRVEPLGPED-----PGSVTVIGAVSPPGGDFSEPVTQNTLRVVKNF WALDKDLAEKRHFPSINWNDSYSGYEN--TLSDYWQENVDEEWNENIQRM KDLLQKDGELQETVQLVGKDALPDRERLTLEIGTLIKQFYLQQNAFHPVD 996Pyrococcus_sp._NA2/1588/1-588 ERAGRVITLGSDN----RVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLTSYSLYTD--AVKDWWHKNVDPEWKKMRDEA MALLQKEAELQEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVD 219Methanoculleus_marisnigri_JR1/1584/1-584 ERAGRVISLNGEG------GSVSVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQRRHFPAINWLNSYSLYLD--ALNEWYDKEVSPEWNPLRAWA MGVLQKEAELQEIVQLVGSDALPDEEQVTIEVARMIREIFLQQNAYDAVD 65Finegoldia_magna_ATCC_29328/1588/1-588 ERAGIVKTLNDNE------GSVTIIGAVSPAGGDFSEPVTENTKRFVNVF LGLDKDLAYSRHYPAINWMSSYSSYVE--KLRDYYESKLGEDVVDLRNQM LKLLYEEDKLKEIVMLVGEDVLPDDQRLILDISNVMKLGFLQQNAFSKVD 38Borrelia_recurrentis_A1/1576/1-576 ERAGVVVLNNGSV------GSVTVGGSVSPAGGNFEEPVTQATLKVVGAF HGLTRERSDARKFPAINPLDSWSKYRG--VVEY------EATEYA RAFLVKGNEVNQMMRVVGEDGVSIDDFVVYLKSELLDSCYLQQNSFDSVD 32Desulfohalobium_retbaense_DSM_5692/1589/1-589 ERAGTIRLHSGDA------GSVTIGGTVSPAGGNFEEPVTQSTLKVVGAF HGLSRQRSDARRYPAIDPLDSWSTYPG--QVDR------EQVQAM HELLQHSNDIDQMMKVVGEEGTPLEDFVIYLKGEFLDQVYLQQDAFDPVD 163Mahella_australiensis_501_BON/1587/1-587 ERTGYVKCMGSQE----REGTLTAVGAVSPPGGDISEPVTQATLRIVKVF WSLDAQLARARHFPAINWLLSYSLYID--NIRDWMNENVAPDWMDLRMQA MRLLQEESSLEEIVRLVGIDAISIRDRWVLEAARSIREDFLHQVAFDEVD 129Eubacterium_limosum_KIST612/1601/1-601 ERAGFVKCLGSED----RYGAISAIGAVSPPGGDISEPVSQATLRIVKVF WSLNANLAYKRHFPAIDWLQSYSLYSD--KMNVYFDKSVETDWSVHVRQT MQILQEEAELDEIVRLVGVDALGFKDRLTLECAQSIREDYLHQSAFDDID 69Sideroxydans_lithotrophicus_ES1/1599/1-599 ERAGRVTTLSGNS------GSITLIGAVSPPGGDFSEPVTSHTKEITKTF WALSKELADARHYPAVDWVESFSGYVA--SCSGWWAENVDPRWESHRATA LGILSQADELARIVNLVGPEALSPQQRWALEGVALFKEGVLQQSALDPVD 214Methanocella_paludicola_SANAE/1579/1-579 ERAGRVITNMGKE------GSVSVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLAQRRHFPAINWLNSYSLYQD--SLKEWYTKNIGGDWNTLKAEA MELLQRESELQEIVQLVGSDALPEDQQLTLEIARMIREYFLQQNAYHEVD 70Nitrosococcus_halophilus_Nc4/1593/1-593 ERAGRVQALGGKV------GSVTLIGAVSPPGGDFSEPVTSHTKEIVGTF WALSKELADARHYPAVSWTESFSDDIP--VAARWWAEHIDKRWQAERAEA MTLLTQAEELSRIVNLVGPEALSGTQRWVLEGAALIKEGVLQQSALDPVD 229Thermococcus_sibiricus_MM_739/1585/1-585 ERAGRVRTLGSDD----RIGSVSVIGAVSPPGGDLSDPVVQNTLRVVKVF WALDADLARRRHFPAINWLTSYSLYVD--SIKDWWQNNVDPEWKAMRDEA MALLQKESELEEIVRIVGPDALPEREKAILLVARMLREDYLQQDAFHEVD 175Clostridium_perfringens_SM101/1591/1-591 ERAGKVVALGKDG----REGAVTAIGAVSPPGGDISEPVTQSTLRIVKVF WGLDAQLAYKRHFPSINWLTSYSLYLE--KMGEWMDAHVADDWSALRTEA MALLQEEANLEEIVRLVGMDALSEGDRLKLEVAKSIREDYLQQNAFHEND 179Clostridium_tetani_E88/1592/1-592 ERAGNVICLGQDG----REGALTAIGAVSPPGGDLSEPVTQATLRIVKVF WGLDSQLAYRRHFPAINWLNSYSLYLD--KVGPWMNENVAEDWVELRQKA MALLQEEANLQEIARLVGIDALSEEDRLKLEVAKSLREDYLQQNAFHDVD 107Aeropyrum_pernix_K1/1597/1-597 ERAGRVKALGSPE----RSGSVTVVGAVSPPGGDFSEPVTSHTTRFIRVF WALDTKLAYSRHYPAINWLMSYSAYVD--LVTQWWHENVDKRWREYRDEA MDILLRESELQEIVRLVGTEGLDEKDKMVLETARLIKDGFLKQNAFDPID 105Ignicoccus_hospitalis_KIN4/I/1596/1-596 ERAGRARIPGRPE----RVGSVTIASAVSPPGGDFSEPVTSHTRRFVRVF WALDASLAYARHYPAINWLVSYSLYVD--TVAKWWHENISPKWKEYRDEM MSILLKEDELKEIVRLVGPESLSEPDKLIIETARIIKEAFLQQNAFDPID 301Thetis_Sea/1-587/1-751 ERAGRVITSGGGD----RKGSITVIGAVSPPGGDFSEPVTQNTLRIIKTF WALDSDLADKRHFPAINWLDSYSGYID--RIEDWWEEEVGANWREYRDKA VSILQEEDELREIVQLVGPDALPDRDQAVLEAARVIREDFLQQNAMHEVD 31Desulfarculus_baarsii_DSM_2075/1581/1-581 ERAGFVRLKSGET------GSVSVIGTVSPAGGNFEEPVTQGTLAVVGAF LGLTWARSNARRFPAIDPLISWSKYLD--QMRDSLDK-LDPAWIASVAKA QRMIFDGNEIKKRMDVVGEEGTSISDFIIYLKAEFLDSVYLQQNAFDKVD 158Clostridium_phytofermentans_ISDg/1589/1-589 ERAGRVISLGSNH----REGALSVIGAVSPPGGDISEPVSQATLRIVKVF WGLDANLAYRRHFPAINWLTSYSLYAD--KMGNWFNSEVNENWTSLRLKI VKLLHDESELEEIVKLVGMDALSSEDRLKLEAARSIREDYLHQDAFHEVD 193Halobacterium_salinarum_R1/1585/1-585 ERAGYFENFNGTE------GSISVIGAVSPPGGDFSEPVTQNTLRIVKTF WALDSDLAERRHFPAINWDESYSLYKD--QLDPWFTDNVVDDWAEQRQWA VDILDEESELEEIVQLVGKDALPEDQQLTLEVARYIREAWLQQNALHDVD 46Spirochaeta_thermophila_DSM_6192/1591/1-591 ERAGVVRLKDGSL------GSVTIGGTVSPAGGNFEEPVTQATLKVVGAF HGLSRERSDARKYPAIHPLESWSKYRG--VTDP------AKTEYA RKVLFDGNEVAQMMKVVGEEGTTLEDFVTYLKAEFLDNVYLQQDAFDPVD 196Halorhabdus_utahensis_DSM_12940/1587/1-587 ERAGYYQNANGTE------GSVTVVGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLADRRHFPAINWDESYSLYRD--QLDPWFEAEVADDWAEIRQWV IDVLDEESDLQEIVQLVGQDALPADQQLTLEVARYIRESYLQQNALHDVD 12Legionella_longbeachae_NSW150/1603/1-603 ERAGIVRCCDQKI------GSLTIVGTVSPAGGNFEEPVTQSTLNAVKTF LGLSYDRAYKRFYPAVDPLISWSRYFE--QLKPWFESNLEPMWITKVMEI TKLLHQGQEIFNMMQVTGEEGITMRDYISYQKSLLFDMTYLQQDAYDSID 236Methanocaldococcus_infernus_ME/1589/1-589 ERAGRVVTLGKDENKKDKIGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVF WALDANLARRRHFPAINWLMSYSLYID--EVTDWWHKNTGPDWRMLRDTA MNLLQKEAELQEIVQLVGPDALPDRERVILEVARMLREDFLQQDAFDDVD 300Thermococcus_litoralis_DSM_5473]/1585/1-585 ERAGRVRTLGSDG----RIGSVSVIGAVSPPGGDLSDPVVQNTLRVVKVF WALDADLARRRHFPAINWLTSYSLYVD--SIKDWWHKNVDPEWKAMRDEA MALLQKESELEEIVRIVGPDALPERERAILLVARMIREDYLQQDAFHEVD 231Pyrococcus_horikoshii_OT3/1964/1-588 ERAGRVVTLGSDY----RVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLTSYSLYVD--AVKDWWHKNIDPEWKAMRDKA MALLQKESELQEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVD 73Anaeromyxobacter_sp._Fw1095/1578/1-578 ERAGRVETLGCGE------GAVTIVGAVSPPGGDLSEPVTQCSLRATGAL WALSADLAHRRHYPAVDWAISFSLEAE--RLRGWFEREAGEGFGALRDEA MKLLQRERELVEVAELVGTESLQDAERLVLESARLLREGFLRQSAYDPVD 194Halalkalicoccus_jeotgali_B3/1587/1-587 ERAGLFDNLNGSQ------GSISAVGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPSINWNESYSLYKD--QLDSWFVENVAGDWPETRQWA VDVLDEESELQEIVQLVGKDALPDDQQLTLEVARYLREGFLQQNAFHDVD 202Haloterrigena_turkmenica_DSM_5511/1587/1-587 ERAGKFQLMNGGE------GSISVVGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPSINWNESYSLYKD--QLDPWWESNVAGDWAETRQWA VDVLDEEGELQEIVQLVGKDALPEDQQLTLEVARFLREAWLQQNALHDVD 997Methanothermus_fervidus_DSM_2088/1585/1-585 ERAGRVITLGSED----KVASVSVIGAVSPPGGDFSEPVTQNTLRICKVF WALDSSLADRRHFPAIDWLQSYSLYVD--SVKDWWKKKIGKDWKAVRDEA MALLQKEAELQEIVQLVGPDALPDIERLTLETARMIREDFLQQNAYHEID 802Parvarchaeum/1583/1-583 ERAGRARILSG------KIGAATIIGAVSPPGGDISEPVSQNTLRVTRVF WALDASLANARHFPSINWLNSYSLYTD--GLNQWYRKNIAEDWPELRNKS LATLQKEAEINEIVQLVGYDALPESDKLILDVAKMLREDYLQQNAFDDID 208Methanosarcina_barkeri_str._Fusaro/1578/1-578 ERAGVAESLCGET------GSITVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQRRHFPAINWLNSYSLYKD--SLNDWFADNVAPDYVPLRERA MEMLQTESELQEIVQLVGSDALPDDQQLLLEITRMLREIFLQQNAFHPVD Ferroplasma_sp._Type_II ERSGNAIISGNPD----RNGSITIVGAVSPPGGDISEPVSQNTLRVTRVF WALDASLASRRHFPSINWLNSYSLYES--ELLPWFSENISKDWPTIYSKM MNILQKESELQEVVQLVGYDALPDREKSVLDVAKIIREDYLQQSAFDDVD 84Caldivirga_maquilingensis_IC167/1595/1-595 ERAGKVKLLNGGL------GSLTIAASVSPPGGDFTEPVTSHTLRFIGAF WPLDARLAYSRHYPAINWLQGFSRYVD--SVASWYSKTVGEDWVELRRIA IEVLTREAELSEIVRILGSEALSEQEKHILNVASMIREGFLKQDAFNPVD 197Haloquadratum_walsbyi_DSM_16790/1585/1-585 ERAGYFENVNGTE------GSVSAIGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPSINWDESYSLYKE--QLDPWFQENVEDDWPEERQWA VDVLDEESELQEIVQLVGKDALPDDQQLTLEVARYLRESYLQQNAFHPVD 232Pyrococcus_abyssi_GE5/11017/1-588 ERAGRVVTLGSDY----RVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLTSYSLYVD--AVKDWWHKNVDPEWKAMRDKA MELLQKESELQEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVD 200Halomicrobium_mukohataei_DSM_12286/1586/1-586 ERAGYFQNINGSE------GSVSVIGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPSINWNESYSLYRD--QLDPWFVENVRDDWPEKRQWG IDTLDEEAELQEIVQLVGKDALPEDQQLTLEVARYLREAWLQQNAFHPTD 143Streptococcus_sanguinis_SK36/1623/1-623 ERAGRVKTLGSTA----REGSITAIGAVSPPGGDISEPVTQNTLRIVKVF WGLDAQLAQRRHFPAINWLSSYSLYLD--EVGAYIDQHEKIAWAEKVTKA MNILQKESELQEIVRLVGLDSLSEKDRLTMNAAKMIREDYLQQNAFDDVD 30Waddlia_chondrophila_WSU_861044/1596/1-596 ERSGVVSLDDGKT------GSVTVGGAVSPAGGNFEEPVTQATLSVVGAF HALSRERSDARRYPAIDPLLSWSKYIK--DVGRKLESQA-EGWSEMVEKA NWFLRNGNEIGKRMEVVGEEGTAIDDMVVYLKAELYDFSYLQQNAFDKED 45Spirochaeta_smaragdinae_DSM_11293/1588/1-588 ERAGVVRLKDGSI------GSVTIGGTVSPAGGNFEEPVTQATLKVVGAF HGLSRERSDARKYPAIHPLDSWSKYSG--VIEP------QKTEYA RQVLHRGDEVGQMMKVVGEEGTSIDDYIIYLKSDFIDYVYLQQNSFDPVD 131Thermanaerovibrio_acidaminovorans_DSM_6589/1569/1-569 ERAGRAICLGSDS----REGAVSIIGAVSPPGGDLSEPVTQNTLRVTKVF WGLDANLAYQRHFPAINWLTSYSLYAE--KLGEYWDNLYDGEWSAYRTEA MGLLEDEDRLKEIVRLVGIDSLSKEERMTLETAKSLREDFLHQNAFHEVD 42Treponema_pallidum_subsp._pallidum_str._Nichols/1589/1-589 ERAGVVRLRSGEK------GSVTIGGTVSPAGGNFEEPVTQATLKVVGAF HGLSRERSDARRYPAVHPLDSWSKYPS--VLDA------RAVAYG RSFLRRGAEVEQMMRVVGEEGTSMEDFLVYLKGSFLDSVYLQQNSFDTVD 77Thermofilum_pendens_Hrk_5/1601/1-601 ERGGRVKALGSPE----RSGSVTVLGAVSPPGGDYNEPVTIHTLRFVGTM WALDTDLAYRRHFPAINWLKSFSQYAD--IIERWWVKNVSPEFPKYRRRA LRLLTVASEIEAIASVVGEGALPDDQRLILLTSEIIKEGFLRQTALSGED 110Thermus_thermophilus_HB27/1578/1-578 ERAGKVITLGGEE------GAVTIVGAVSPPGGDMSEPVTQSTLRIVGAF WRLDASLAFRRHFPAINWNGSYSLFTS--ALDPWYRENVAEDYPELRDAI SELLQREAGLQEIVQLVGPDALQDAERLVIEVGRIIREDFLQQNAFHEVD 203Natrialba_magadii_ATCC_43099/1587/1-587 ERAGKFQNINDTE------GSVTAIGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPSINWNESYSLYRQ--QLDPWFRDNVADDWPEVRQWA VDVLDEEDELQEIVQLVGKDALPEDQQLTLEVARYLREAWLQQNAFHDVD 93Sulfolobus_acidocaldarius_DSM_639/1592/1-592 ERAGRVIALGKPE----RFGSVSIASAVSPPGGDFTEPVTSNTLRFVRVF WPLDVSLAQARHYPAINWIQGFSAYVD--LVASWWHKNVDPTWFEMRSVL VKILLREDELRQIVRLVGPESLSDKDKLILEASKLIRDAFLKQNAFDDID 35Borrelia_burgdorferi_B31/1575/1-575 ERAGIVVLNNGDI------GSVTVGGSVSPAGGNFEEPVTQATLKVVGAF HGLTRERSDARKFPAISPLESWSKYKG--VIDQ------KKTEYA RSFLVKGNEINQMMKVVGEEGISNDDFLIYLKSELLDSCYLQQNSFDSID 55Spirochaeta_smaragdinae_DSM_11293/1592/1-592 ERAGYVDTLSGSD------GSVSIIGAVSPPGGDFSEPVTQHTKRFVRCF WGLDRQLANARHYPAISWLESYSEYLD--EITSWWERRVGEEWTAERREM MDLLQREVRLQQVVKLVGPDALPDSQRFIIEVCTLFKNAFLQQNAFDDVD 29Candidatus_Protochlamydia_amoebophila_UWE25/1593/1-593 ERSGVVSLRHGKP------GSITIGGAVSPAGGNFEEPVTQATLSVVGAF LGLSRARSDSRRYPAIDPLLSWSKYVD--TVGNELSHQV-DGWDQMVKRA RHILFNGNEIGKRMEVVGEEGISMEDMLTYLKAELYDFSYLQQNAFDKED 198Haloferax_volcanii_DS2/1586/1-586 ERAGYFTTVNGEE------GSVSVIGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPAINWNESYSLYQE--QLDPWFVENVEDDWAEERQWA VDVLDEENELQEIVQLVGKDALPEDQQLTLEIARYLREAYLQQNAFHPTD 302Thermoplasmatales_archaeon/1-575 ERAGNSVVLGQKQ----RTGSVTIIGAVSPPGGDISEPVSQNTLRVTRVF WGLDASLAQRRHFPSVNWLTSYSLYSE--DVRKWFEKNVSQEWHSQYLRS MEILQEESELQEVAQLVGYDALPENEKEVLDVARAIREYFLQQSAFDDVD 62Halothermothrix_orenii_H_168/1590/1-590 ERAGYVKTLGQD-----REGSISLIGAVSPPGGDFSEPVTQNTKRYVRCF WALDKSLASARHFPAVNWLESYSEYLE--DLSGWLKENINEDWIKLRNMA MELLKEEDRLQEIVKLVGEDVLPDNQRLILEVARLIKIGFLQQNAFSKVD 115Truepera_radiovictrix_DSM_17093/1579/1-579 ERGGRVTTLAGEQ------GAVSIIGAVSPAGGDFSEPVTQSTLRITGCF WALDASLARRRHFPAINWNRSYTLFAD--ILEPWYRENVAEDYPELRAQA VALLQREAELQEVVQLVGPDALQDNERLIIEIGRILRQDFLQQNGFDPVD 16Chlamydophila_pneumoniae_CWL029/1591/1-591 ERGGAITTKDGSE------GSLTICGAVSPAGGNFEEPVTQSTLAVVGAF CGLSKARADARRYPSIDPLISWSKYLN--QVGQILEEKV-SGWGGAVKKA AQFLEKGSEIGKRMEVVGEEGVSMEDMEIYLKAELYDFCYLQQNAFDPVD 213Methanohalophilus_mahii_DSM_5219/1576/1-576 ERAGAVHSLAGLD------GSITVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQKRHFPAINWLTSYSLYND--RLAGWFTENVGSDWNELRDNA MDILQEEAELQEIVQLVGSDALPEDQQLTLEIARMLREYFLQQNAFHPVD 78Candidatus_Korarchaeum_cryptofilum_OPF8/1595/1-595 ERAGRVKVIGNRD----EIGSIAIIGAVSPSGGDLNEPVTQHTMRYVGAF WALDKDLAFKRHFPSINWLRSFSKYAP--ALKNWWINATGHDMVEYRDRA MSLLQAASSLEEVARVVGEAALSDENRLVLLSAELIKEGFLVQSAYHEVD 19Chlamydia_muridarum_Nigg/1591/1-591 ERGGAVKMKDGSE------GSLTICGAVSPAGGNFEEPVTQATLSVVGAF CGLSKARADARRYPSIDPMISWSKYLD--SVAEILEKKV-PGWGDSVKKA SRFLEEGAEIGKRIEVVGEEGISMEDIEIFLKSELYDFCYLQQNAFDAED 52Thermanaerovibrio_acidaminovorans_DSM_6589/1594/1-594 ERAGRVITLGGET------GSVTIIGAVSPPGGDFTEPVTRHTKRYIRCF WGLDKSLANARHFPAISWLDSYSEYAR--EVEDWFAAQVDPRWKDLRTRA SQILAEDNKIQQIIRLVGEDVLPDEQKLVAFTAFLIKNGYLQQSAFGP-D 223Methanopyrus_kandleri_AV19/1592/1-592 ERAGRVVCLGSDD----RVGSVTVVGAVSPPGGDFSEPVTQNTLRIVKVF WALDSKLADRRHFPAINWLQSYSLYLD--DVEKWWHEEIGGDWRELRDEA MEILQRESELEEIVQLVGPDALPESERLILEVARMIREDFLQQNAFHEVD 189Leptotrichia_buccalis_DSM_1135/1597/1-597 ERAGKVICLGKDG----REGALTVIGAVSPPGGDISEPVSQATLRIVKVF WGLDANLAYRRHFPAINWLNSYSLYQG--KVDNWMDQNVGTKFSKNRARA ISLLQEENSLQEIVRLVGKDTLSEKDQLKLEIAKSIREDYLQQNAFMESD 191Fusobacterium_nucleatum_subsp._nucleatum_ATCC_25586/1382/1-584 ERAGLVECLGNGE-----EGALTVIGAVSPPGGDISEPVSQSTLRIAKVF WGLDYALSYRRHFPAINWLNSYSLYQA--KMDKYKEEEIDTDFPKFRIEA MALLQEEAKLQEIVRLVGRDSLSELDQLKLEITKSLREDFLQQNAFHEVD 4Bacteroides_vulgatus_ATCC_8482/1584/1-584 GRAGYVHLNNGET------GSITFIGTVSPAGGNLKEPVTENTKKVARCF YALEQERADRKRYPAVNPIDSYSKYLEYPEFEEYIAKRINGEWIGKVNEI KTRLLRGKEIAEQINILGDDGVPVEYHVIFWKSELIDFVILQQDAFDEVD 95Metallosphaera_sedula_DSM_5348/1591/1-591 ERAGRVRTLGNPE----RYGSVTLASAVSPPGGDFTEPVTSTTLRFVRVF WPLDVSLAQARHYPAINWLQGFSSYVD--LVSDWWIKNVDPDWRIMRDFM VRTLLREDELKQIVRLVGPESLAEKDKLTLEVARLIKEAFLKQNAYDDID 44Treponema_denticola_ATCC_35405/1589/1-589 ERAGIVRLSDGSK------GSVTIGGTVSPAGGNFEEPVTQATLKVVGAF HGLSRERSDARKYPAIHPLDSWSKYPS--VLPL------EQVKYG RSFLRRGTEVEQMMKVVGEEGTSIEDFIIYLKGDLLDAVYLQQNSFDKVD 90Desulfurococcus_kamchatkensis_1221n/1584/1-584 ERSGYVKTIGSAE----RSGSLTIMGAVSPPGADFSEPVTQSTLRLIRAL YALDVNLAYRRHYPSINWLTSFSLYID--NVTEWWSK-ISREYPYLRERF MKILQRETELEELVRLVGADALPVEDRLTLEVARIIREDFLQQDAFSLVD 57Thermotoga_sp._RQ2/1586/1-586 ERAGLCENLNGTT------GSVTIIGAVSPPGGDFSEPVTQHTKRFVRCF WALDRNLAYSRHYPSINWITSYSEYAE--VLEDWFKENVNEKWPEYRQEA IDILVEDDRLQQIIKIMGEDVLPDDQKLTVLVARIIKVGVLQQNAFSEVD 803Pyrococcus_yayanosii/1587/1-587 ERAGRVVTLGSDY----RVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLTSYSLYVD--SIKDWWHQNVDPEWKSMRDWA MALLQKEAELQEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVD 228Methanobrevibacter_smithii_ATCC_35061/1584/1-584 ERAGRVNTIGTTS----DVASITVVGAVSPPGGDLSEPVTQNTLRICKVF WALDASLADKRHFPSIDWLQSYSLYVD--SIEGWWAENVAADWRETRDQA MILLQKESELQEIVQLVGPDALPEADQATLETTRMLREDFLQQNAFDDID 13Cyanothece_sp._PCC_8801/1607/1-607 ERAGMIRTNDGSI------GSLTMIGTVSPAGGNFEEPVTQSTLSTVKAF LGLSAERAYKRFYPAVDILISWSRYLQ--QLAPWFEKNVSPGWVKRVEEM IDLLKEGHRIDQMMQVTGEEGVTLEDFITSQKALFIDMVYLQQDAFDEVD 168Thermoanaerobacter_pseudethanolicus_ATCC_33223/1590/1-590 ERAGRVICLGSDN----REGALTVVGAVSPPGGDLSEPVTQATLRVVKVF WALDSELAYARHFPAINWLTSYSLYSD--VVEDYMNKNVSSDWGELRSEA MRLLQEEASLQEIVRLVGIDALSTRDRLVLEVARSIREDFLHQNAFHEVD 26Chlamydophila_felis_Fe/C56/1588/1-588 ERGGAVRMKDGSE------GSLTICGAVSPAGGNFEEPVTQATLSVVGAF CGLSKARADARRYPSIDPMISWSKYLD--QVGEILENKV-HGWGESVKKA NHFLREGSEIGKRMEVVGEEGISMEDMEIYLKAELYDFCYLQQNAFDAVD 80Thermoproteus_neutrophilus_V24Sta/1594/1-594 ERAGRVVLIGSKE----RVGSLTIAASVSPPGGDFTEPVTSNTLRFIGAF WPLSPRLAYSRHYPAIDWLAAFSRYVD--TVEVWWSKNISTEWRRIRDTL QSLLVKEAELQEIVRILGTEALSEYEKHVLNVAFMIREGFLKQDAFNPVD 114Deinococcus_radiodurans_R1/1582/1-582 ERAGAVKTLAGED------GAVSVIGAVSPAGGDMSEPVTQATLRITGAF WRLDAGLARRRHFPAINWNGSYSLFTP--ILDKWYRENVGPDFPELRQRI GSLLQQEAALQEVVQLVGPDALQDNERLIIETGRMLRQDFLQQNGFDPVD 122Methanocella_paludicola_SANAE/1579/1-579 ERAGRMVCLGSGD----RVGSVTVVGAVSPPGGDFSEPITQNTLRTVGTF WALDTSLAYRRHFPSVNWIKSYSLYLD--GVEDWYVENVSREWRSLRDKT MYLLQKEVELQEIVQLVGPDALPEGEKAILEVTRMIREDFLQQSAYSDSD 217Methanoplanus_petrolearius_DSM_11571/1582/1-582 ERAGRVETLNHDF------GSITVIGAVSPPGGDFSEPVTQNTLRIVKCF WALDAKLSQRRHFPAINWLNSYSLYLD--SLSSYYDEKISPEWNPLRTWA MAVLQKESELQEIVQLVGSDALPDEEQVTIEVARLLREVFLQQNAFDPVD 998Thermoplasma_acidophilum_1221B2/1590/1-590 ERSGRARLVSPDE----RYGSITVIGAVSPPGGDISEPVSQNTLRVTRVF WALDAALANRRHFPSINWLNSYSLYTE--DLRSWYDKNVSSEWSALRERA MEILQRESELQEVAQLVGYDAMPEKEKSILDVARIIREDFLQQSAFDEID 204Methanosaeta_thermophila_PT/1576/1-576 ERAGRAEVLAGGE------GSVAVVGAVSPPGGDFTEPVTQNTLRIVKVF WALDSRLTQRRHFPSINWLDSYSLYEK--DLESWYAENVAPDWNQLKRRA MAILQENAELEEIVMLVGSDALPEDQQLTLEVARMIINFWLAQSAFHPVD 218Methanospirillum_hungatei_JF1/1582/1-582 ERAGRVNTLNKDF------GSVTVIGAVSPPGGDFSEPVTQNTLRIVKCF WALDAKLSQRRHFPAINWLNSYSLYLD--TLSQYYDENVSPEWNPLRTWA MEVLQKEAELQEIVQLVGSDALPDEEQVTIEVARMLREIFLQQNAFDPVD 221Candidatus_Methanosphaerula_palustris_E19c/1588/1-588 ERAGLVKTLNGQE------GSVSVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQRRHFPAINWLDSYSLYLD--TLHDWYDREVSPEWNKIRSWA MEILQKEAELQEIVQLVGSDALPEAEQITIEVARMIREIFLQQNAYDAVD 171Anaerococcus_prevotii_DSM_20548/1585/1-585 ERAGKVEVLGSRN----DIGSLSVIGAVSPPGGDLSEPVTQATLRIVKVF WGLDYDLSYQRHFPAINWLSSYSLYQD--KMDKYIDSEVDEEFSKNRKRS MSLLQQESGLQEVVRLVGRDALSDDDKLKLDVTKSIREDYLQQNAFHDVD 120Ferroplasma_acidarmanus_fer1/1589/1-589 ERSGNAQIIAQDE----RTGSITLIGAVSPPGGDLSDPVVQNTLRVTRAF WALDASLASRRHFPSINWLNSYSLYLD--SLSGWFKSNVNPEWPQMHSLM MGILQKESELQEIVQLVGYDSLPENQKNVLDIAKIIREDFLQQNAFDDTD 119Thermoplasma_volcanium_GSS1/1776/1-590 ERSGRARLVSPED----RFGSITVIGAVSPPGGDISEPVSQNTLRVTRVF WALDASLANRRHFPSINWLNSYSLYTE--DLRHWYDENVAKDWGSLRSQA MDILQRESELQEVAQLVGYDAMPEKEKSILDVARIIREDFLQQSAFDEID 3Brachyspira_murdochii_DSM_12563/1587/1-587 ARAGFVYLNNGET------GSITFIGTVSPAGGNLKEPVTESTRKAARCF YALSQKRADSKRYPAVDPLDSYSKYIEYPEFIEFSDTNIEKGWSEKVIKA KDIARRGQEANDQISILGDDAVPLEYHQRLWKAELIDFVILQQDAFDKVD 59Anaerococcus_prevotii_DSM_20548/1582/1-582 ERAGYFKNLNGSE------GSVTLIGAVSPSGGDFSEPVTENTRRCVNVF LGLDRKLAYSRHYPAINWLSSYSNYIE--KIRAYYEERLGKDIIAIRNEL MNVLLEEDEVRSIMMLVGEDALSNSQKNILDVSGLIRNGFLQQNAYNDID 164Clostridium_thermocellum_ATCC_27405/1589/1-589 ERAGRVVCLGSDG----REGALTAIGAVSPPGGDLSEPVTQATLRIIKVF WGLDSSLAYRRHFPAINWLQSYSLYLD--IIGKWISENISRDWETLRSDT MRILQEEAELEEIVRLVGVDALSPSDRLTLEAAKSIREDYLHQNAFHEVD 237Methanocaldococcus_vulcanius_M7/1587/1-587 ERAGRVITLGKDN----RQGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVF WALDANLARRRHFPAINWLQSYSLYID--DVTDWWLTNTGPDWRQLRDEA MGLLQKEAELQEIVQLVGPDALPDRERVILEVARMLREDFLQQDAFDEVD 124Dictyoglomus_thermophilum_H612/1589/1-589 ERAGLVKCLGKED----KIGSVSIIGAVSPPGGDLSDPVVQATLRVTKVF WGLDARLAFSRHFPAVDWLVSYSLYLE--PVSKFWKENVAEDFYDLRQEA MAILQREAELQDIVRLVGMEALSPQDRLTLETARSIREDFLHQNALHEVD 153Streptococcus_pyogenes_M1_GAS/1591/1-591 ERAGRVRTLGSQE----REGTITAIGAVSPPGGDISEPVTQNTLRIVKVF WGLDAPLAQRRHFPAINWLTSYSLYQD--DVGSYIDRKQQSNWSNKVTRA MAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFDSVD 41Fibrobacter_succinogenes_subsp._succinogenes_S85/1585/1-585 ERGGVVRLKDGST------GSVTICGSVSPAGGNFEEPVTQATLKVVGAF LGLSRERSDQRRFPAIHPLDSWSKYEG--IIDS------KKVAQA RSILANGVDVNNMMKVVGEEGTSIEDFIVYLKSEYLDAVYLQQDAYNEID 220Candidatus_Methanoregula_boonei_6A8/1588/1-588 ERAGLVETLNHQS------GSVSVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQRRHFPAINWLNSYSLYLD--ALHDWYDKNVSPDWNKLRSWA MGVLQKEAELQEIVQLVGSDALPETEQITIEVARMIREIFLQQNAYDAVD 79Pyrobaculum_islandicum_DSM_4184/1593/1-593 ERAGRVVLMGSKE----RIGSLTIAASVSPPGGDFTEPVTSNTLRFIGAF WPLSPRLAYSRHYPAIDWLLAFSRYVD--TVEVWWSKNISPDWRKIRDTL QSILVKEAELQEIVRILGTEALSEYEKHILNVAYMIREGFLKQDAYNPVD 177Clostridium_botulinum_E3_str._Alaska_E43/1592/1-592 ERAGKVECLGNDG----RIGSITAIGAVSPPGGDISEPVSQSTLRIVKVF WGLDAQLAYQRHFPTINWLTSYSLYAD--TIDKWMNGNVAENWGALRLEA MTILQDEAQLQEIVRLVGIDALSEKDRLKLDVAKSIREDYLQQNGFHEID 48Geobacter_uraniireducens_Rf4/1524/1-524 ERAGVVETMNCGI------GSLSMILSVSPPGGDFTEPVTQACLRTAGAF FMLDTALAHRRHFPAINWFQSYSLYER--NIVRHFTAEISPRWEELLRRC REMLQREESLREVAEIVGIEGLQDADRLLMKVAERIRLEFLCQNAYTE-D 211Methanohalobium_evestigatum_Z7303/1578/1-578 ERAGYVKSNSGYD------GSITVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLSQRRHFPAINWLTSYSLYTE--SLSDWLSENVASDWIDLRNYA MDILEEESELQEIVQLVGSDALPESQQLKLEVARMFREYFLQQNAFHEID 230Pyrococcus_furiosus_DSM_3638/11013/1-588 ERAGRVVTLGSDY----RVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLTSYSLYVD--AVQDWWHKNVDPEWRRMRDKA MELLQKEAELQEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVD 47Stackebrandtia_nassauensis_DSM_44728/1575/1-575 ERAGVVETLGGRI------GSVTVVGAVSPPGGDLTEPVTAYTERFVRCR WTLDRDLAYARHYPSVSWSGSFCRDAE--TIGAWHAAHGDRAWWARRERL TTVLSEADRLSALAELVGTTALPPHERVILLGGRLIREAVLQQSALSATD 199Haloarcula_marismortui_ATCC_43049/1586/1-586 ERAGYFENINGTE------GSVSVIGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPSINWNESYSLYRD--QLDPWFEDNVRADWPETRQWA IDTLDEEAELQEIVQLVGKDALPEDQQLTLEIARYLREAWLQQNAFHDVD 227Methanobrevibacter_ruminantium_M1/1584/1-584 ERAGRVITLGQDP----KESSVTVVGAVSPPGGDLSEPVTQNTLRICKVF WALDASLADKRHFPSINWLQSYSLYVE--SITNWWQENASEEWRANRDQA MVLLQKESELEEIVQLVGPDALPDSEQVVLETTRMLREDFLQQNAYDDVD 10Parabacteroides_distasonis_ATCC_8503/1585/1-585 ARAGFVHLNNNAT------GSVTFIGTVSPAGGNLKEPVTENTKKVARCF YALEQERADRKRYPAVNPIESYSKYIEYPEFQEYIAKHISPEWIEKVNEI KTRMLRGKEISEQINILGDDGVPVEYHVTFWKSELIDFVILQQDAFDAID 195Halorubrum_lacusprofundi_ATCC_49239/1591/1-591 ERAGYFENINGTE------GSVSAIGAVSPPGGDFSEPVTQNTLRIVKTF WALDADLAERRHFPSINWNESYSLYKD--QLDPWFENEVADDWAEKRQWA VDVLDEETELQEIVQLVGKDALPDDQQLTLEVARYLREAYLQQNAFHPVD 64Thermosediminibacter_oceani_DSM_16646/1587/1-587 ERAGYFKNLNGSE------GSITIIGAVSPAGGDFSEPVTQATKRVVGAF LALDKELAYARHFPSINWLLSYSGYVK--MLEDWFSDNVAPDLLELRAKM LGLLREENKLMDIVKLVGEDVLPDDQRLVLEIARVLKMAYLQQNAYHKED 144Streptococcus_gallolyticus_UCN34/1591/1-591 ERAGRARVLGQEG----REGTITAIGAVSPPGGDISEPVTQNTLRIVKVF WGLDAPLAQRRHFPAINWLTSYSLYKD--EVGKYVDKVQKTNWAAKVTQA MNILQRESSLEEIVRLVGIDSLSEADRLTMEIAKQIREDYLQQNAFDSVD 165Thermosediminibacter_oceani_DSM_16646/1590/1-590 ERAGRVICLGSDN----REGALTVVGAVSPPGGDLSEPVTQATLRVVKVF WALDSQLAYARHFPAINWLTSYSLYSD--VVENYMNKNVSSDWGELRSEA MKLLQEEASLQEIVRLVGIDALSTRDRLVLEVARSIREDFLHQNAFHEVD 172Clostridium_cellulovorans_743B/1591/1-591 ERAGKVVCLGKDE----RIGTITAIGAVSPAGGDVSEPVTQSTLRIVKVY WGLDAQLAYRRHFPSINWLSSYSLYAD--RIGNYLDEVVKGNWSNLRTRA MAILQEEANLEEIVRLVGIDALSEKDRLTLEVAKSIREDYLQQNAFHEID 201Natronomonas_pharaonis_DSM_2160/1585/1-585 ERAGKFQNMNGSE------GSISVIGAVSPPGGDFSEPVTQNTLRIVKCF WALDADLAERRHFPSINWNESYSLYKE--QLDPWWRENVHEEFPEVRQWA VDVLDEEDELQEIVQLVGKDALPEDQQLTLEVARYIREAWLQQNAFHDVD 178Clostridium_novyi_NT/1591/1-591 ERAGKVVCLGDDE----REGAITAIGAVSPPGGDLSEPVTQATLRIVKVF WGLDAQLAYRRHFPAINWLNSYSLYLD--SIGRWMDRNVSEEWVDLRTRA MTILQEEANLEEIVRLVGIDALSESDRLKLEVAKSIREDYLMQNAFHDVD 141Streptococcus_mitis_B6/1591/1-591 ERAGRSQVLGLPE----REGTITAIGAVSPPGGDISEPVTQNTLRIVKVF WGLDAPLAQRRHFPAINWLTSYSLYKD--SVGTYIDGKEKTDWNSKITRA MNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFDSVD 109Methanospirillum_hungatei_JF1/1588/1-588 ERAGRVSLLGSGD----HEGSISVIGAVSPPGGDFSEPVTQNTLRIVKVF WALDADLAYQRHFPAINWLMSYSLYSP--IAGIWWEEHIGPEFIRMKQEM MEILQRENELEEIIRLVGPETLPESDRLLLLKAEILRESYLMQYAFDEFD 49Aminobacterium_colombiense_DSM_12261/1588/1-588 ERAGRAKLLGLPE----REGSVTVINAVSPAGGDFSEPVTQASLRLSGAF WALDKRLAQQRHFPAINWQQSYTLYEG--SLASVFERELGSQWKVLKSYL KEMLDREKVLTDLVQLVGRDGLSEEDKWLLAHVETIKVVFLQQNAYSDAD 118Thermoplasma_acidophilum_DSM_1728/1764/1-590 ERSGRARLVSPDE----RYGSITVIGAVSPPGGDISEPVSQNTLRVTRVF WALDAALANRRHFPSINWLNSYSLYTE--DLRSWYDKNVSSEWSALRERA MEILQRESELQEVAQLVGYDAMPEKEKSILDVARIIREDFLQQSAFDEID 94Sulfolobus_tokodaii_str._7/1595/1-595 ERAGRVIALGNPE----RYGSVTIASAVSPPGGDFTEPVTSNTLRFVRVF WPLDVSLAQARHYPAINWIQGFSAYVD--LVAQWWHKNVDPNWKEMRDTM MKVLIREDELRQIVRLVGPESLAEKDKLVLEAAKLIKDAFLKQNAYDDID 102Sulfolobus_islandicus_M.16.27/1592/1-592 ERAGRVKTVGKPE----RFGSVTVASAVSPPGGDFTEPVTSQTLRFVKVF WPLDVSLAQARHYPAINWLQGFSAYVD--LVANWWNTNVDPKWREMRDMM VRTLIREDELRQIVRLVGPESLAEKDKLVLETARLIKEAFLKQNAYDDID 53Treponema_pallidum_subsp._pallidum_str._Nichols/1605/1-605 ERAGRVETCVARE------GSVSIIGAVSPLGGDFSEPVTQHTKRFIRCF WALDRELAHARHYPAIGWIDSYSEYAQ--EVSAWWSK-YDPRAGALRAAA LDLLRKEQRLQQIVRLVGPDALPGEDRLVLMVCEMIKGGFLQQNAFDPTD 106Hyperthermus_butylicus_DSM_5456/1601/1-601 ARAGRVKVPGRPE----RLGSVTIVGAVSPPGGDFTEPVTANTKRFIRVF WALDARLAYSRHYPAINWLVSYSAYVE--TVAKWWHENISPKWLEYRNEA YEILLREDELREIVRLVGTEGLSEKDKLILEIANIIKTGFLQQNAFDPVD 104Acidilobus_saccharovorans_34515/1601/1-601 ERAGRVRTLGEPS----RFGSVTVIGAVSPPGGDFTEPVTTHTRRFTKVF WALDTALAYSRHYPAINWITSYSAYSD--TVAEWWHKNVDPRWSEYRQEV MNILIRENELREIVRLIGTEGLSEQDKLILETARLIKEGLLKQNAFDPID 58Acholeplasma_laidlawii_PG8A/1583/1-583 ERAGMMINQNRST------GSVSIIGAVSPQGADFSEPVTQNTKRFVRAF WALDKSLAHQRHFAAINWNLSYSEYIY--DLSSWYDNTLDPKFLKYRSRI LSILAEEYKLNEIASLMGSDVLPDNQKLTIEIGRVIRTGFLQQNAFNAVD 130Aminobacterium_colombiense_DSM_12261/1598/1-598 ERAGRAICLGSDG----REGSVTAIGAVSPPGGDLSEPVSQNTLRVTKVF WGLDASLAYQRHFPAINWLLSYSLYTD--TLDKYWDSQFDDEWSALRVEG MSLLEEEDQLREIVRLVGVDALSKEERMVLETSKSLREDFLHQNAFHEVD 243Methanococcus_vannielii_SB/1586/1-586 ERAGRVDCLGSED----RQGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVF WALDANLARRRHFPAINWLTSYSLYIN--DIAGWWKKNTGEDWRVLRDEA MGLLQKEAELQEIVQLVGPDALPDRERVILEIARILREDFLQQDAYHEVD 207Archaeoglobus_profundus_DSM_5631/1580/1-580 ERAGRVKTLSGHI------GSVTVVGAVSPPGGDFSEPVTQNTLRIVKVF WALDAKLAARRHFPAINWLQSYSLYAD--ILRPWFEQNVAPDWGELRRWA MEVLQEEANLQEIVQLVGSDALPDAQRVLLEVARMIREIYLIQYAYDPVD 999Ferroplasma_acidarmanus/1589/1-589 ERSGNAQIIPQDE----RTGSITLIGAVSPPGGDLSDPVVQNTLRVTRVF WALDASLASRRHFPSINWLNSYSLYLD--SLSGWFKSNVNTEWLQAHSLI MGILQKEAELQEIVQLVGYDSLPEKQKNILDIAKIIREDFLQQNAFDDTD 116Meiothermus_ruber_DSM_1279/1578/1-578 ERAGKVITLAGEQ------GAVSVIGAVSPAGGDFSEPVTQSTLRITGGF WALDAQLARARHFPAINWARSYSLFLN--ILEPWYRENVAPDYPEVRTQI ITLLQREAELQQVVQLVGPDALQDAERLALEIGRIAREDFLQQNGFDPVD 123Methanospirillum_hungatei_JF1/1584/1-584 ERAGRVICAGSEE----KTGSVTIIGAVSPPGGDFSEPITQNTLRIAGTF WALDANLAYRRHYPSVNWIRSYSLYLE--DVEDWFSEKVARDWYQFRGRA MYILQKEVELQEIVQLVGPDALPDKEKVVLEIAKIIREDFLQQSAYSDDD 226Methanothermobacter_thermautotrophicus_str._Delta_H/1584/1-584 ERAGRVTTIGSED----KIASVSVVGAVSPPGGDLSEPVTQNTLRICKVF WALDASLADKRHFPSIDWLQSYSLYID--SVQEWWASNVDPEWRKFRDEA MALLQKEAELQEIVQLVGPDALPDRERITLETTRMIREDFLQQNAYHEVD 241Methanococcus_aeolicus_Nankai3/1586/1-586 ERAGRVACMGHDE----KQGFVCIVGAVSPPGGDFSEPVTSNTLRIVKVF WALDANLARRRHFPAINWLQSYSLYLD--DIEDWWKENTAEDWREIRDEA MNLLQKEAELQEIVQLVGPDALPDKERVILEVSRMLREDFLQQDAFSDID 234Thermococcus_gammatolerans_EJ3/1585/1-585 ERAGRVVTLGSEP----RVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLRSYSLYLD--AVQDWWHKNVDPEWRKMRDTA MALLQKEAELQEIVRIVGPDALPDREKAILIVTRMLREDYLQQDAFDEVD 224Methanosphaera_stadtmanae_DSM_3091/1585/1-585 ERAGRVTTIGSHK----AEASVTVVGAVSPPGGDLSEPVTQNTLRIAKVF WALDASLADRRHFPSINWLNSYSLYVD--SITNWWNSQIGSDWRDLRNTA MALLQKESELNEIVQLVGPDALPQKDRVTLESARMLREDFLQQNAFDDTD 233Thermococcus_onnurineus_NA1/1585/1-585 ERAGRVITLGSDE----RIGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVF WALDADLARRRHFPAINWLRSYSLYID--SIQDWWHKNVDPEWRAMRDRA MELLQKEAELQEIVRIVGPDALPDREKAVLIVARMLREDYLQQDAFDEVD 66Eubacterium_limosum_KIST612/1595/1-595 ERAGSVDTLCGEE------GSISIVGAVSPAGGDFSEPVTENTKRYVNTF LALDKNLAYARHYPAINWMESYSGYVK--LLTDWYEDNVAEDSIQLRNKM LDLLFQENKLQEMVMLIGEDALPDDQRWVLSIAKLIRVGFLQQNTFDDTD 188Streptobacillus_moniliformis_DSM_12112/1588/1-588 ERAGKVVCLGEDE----RIGALTVIGAVSPPGGDISEPVSQSTLRIVKVF WGLDAQLAYRRHFPAINWLNSYSLYQT--KVDEWMDKNVDEKFSANRTMA MKLLQEEAGLQEIVRLVGKDTLSFEDQLKLEAAKSIREDYLQQNAFHEQD 140Streptococcus_pneumoniae_TIGR4/1591/1-591 ERAGRSQVLGLPE----REGTITAIGAVSPPGGDISEPVTQNTLRIVKVF WGLDAPLAQRRHFPAINWLTSYSLYKD--SVGTYIDGKEKTDWNSKITRA MNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFDSVD 60Clostridium_phytofermentans_ISDg/1588/1-588 ERAGYVQNLNGSE------GSVSIIGAVSPQGGDFSEPVTQNTKRFVRCF FALDKSLAYARHFPAIQWLTSYSEYLN--DLAPWYAKNVGNNFMDYRNEI LRILTEESKLNEIVKLIGSDVLPDDQKLTLEIARVIRLGFIQQNAYHASD 601 25Chlamydia_trachomatis_B/TZ1A828/OT/1591/1-591 CYCPFDRQIELFSLMNHIFNSRFCF-DCPDNARSFFLE--LQSKIKTLNG QKFL------SEEYQKGLEVIYKLLESKMVQTV------5 801Micrarchaeum/1586/1-586 TYTSMHKQYLMLSSIMELAAEQAKS-LERGVTLEQLSK--L-DVRQKIAR MKYVKDGDI-DAYYKDVMDSIAK-IKGMQPAVK------222Aciduliprofundum_boonei_T469/1582/1-582 TYCPADKQYLMLKLILKFHSLITRA-VESGVPMDKIRK--L-SVKEDLAY MGRIPNETY-KEEFAKIEEKIESEIDRLIREVK------127Acholeplasma_laidlawii_PG8A/1592/1-592 TYSSLDKQFRLLDMIILFNDLAVHA-IEHEMSFKDIER--L-AVKEQIGR FKYVEEKDM-LNTYEEVVKEMHQQFSGLRKE------85Vulcanisaeta_distributa_DSM_14429/1603/1-603 VKSLPPKQYWLLRLIITFYRKGLEA-INRGVPASALRE--L-DVVKRLAR LRMEVRNEE-YKKLVDYEQELISAIENLVKQYETQRVTATA------210Methanosarcina_mazei_Go1/1578/1-578 TYSPFEKQYKIMKAIMKWGDAAMDA-LKSGVLSSEILK--M-QSKDELPK VKFEEDF---EGSLNAVLAKMDKEFAALGGK------8Porphyromonas_gingivalis_ATCC_33277/1584/1-584 CNSSLERQELMLSRITDICRTEFDF-EDFLEVSDYFKR--MINTFKQVNY SQFK------SAEFDKYNKELDAILAERIK------216Methanocorpusculum_labreanum_Z/1581/1-581 TYCDLPKQLDMFKMIRTYADLAYAA-QAAGVPPSQILA--V-KAKNEMPQ VKFTKDY---KPVLDKIYAGMEAEFKALRA------212Methanococcoides_burtonii_DSM_6242/1578/1-578 TYCPFDKQYKLLKSITRYGELATAA-LESGVPMNKIIT--I-KSKDELAK VKFEENF---DAALDVVMKKMDEEFAQIGGN------242Methanococcus_voltae_A3/1585/1-585 SYCSPAKQYNMLKVIMTYYNKALEV-VAKGANPADISN--V-SVKGDIAR MKYVEEQEFINTKAPEMISKMDAELNKLI------132Enterococcus_faecalis_V583/1595/1-595 TFTSRTKQAKMLQLILTFGEEGQKA-LSLGTYFSELMAGTV-EIRDRIAR SKYLPEEEL-EK-LDRLQAEIKTTIKEIIAEGGMTND------63Clostridium_sticklandii_DSM_519/1594/1-594 TFVPILKQYEMLKVIYHLHTRALAA-VKANLPLSEIRN--P-EIFDEVIK MKYTVPNED-LSLIDDLHYRIDSYFDELATRYASITEVI------215uncultured_methanogenic_archaeon_RCI/1579/1-579 TYCSLDKQLKMLKSIMQFGAYARTA-LASGVPMSKILN--L-NSKNDLAK VKFEANY---DAYLTKVNDDMKKEFKSLEAA------121Picrophilus_torridus_DSM_9790/1922/1-589 TYCSIKKQYMMLKIIKTVYEMQMNA-LNHGMKISQITS--I-PARSKISR MKEVSEQDF-PAFYKNIIKEINDEYNSMIEVGGVNA------209Methanosarcina_acetivorans_C2A/1578/1-578 TYSPFAKQYKIMQAIMKYGDAAMDA-LKSGVPASEIIK--M-ESKDELPK VKFEEDF---DGSLSAVLAKMDKEFAALGGR------128Acidaminococcus_fermentans_DSM_20731/1590/1-590 TYTSLEKQYDMLKLIITWYEKALDA-MTKHVKLDKLID--M-PIREQIGR AKYIPEKER-KEKFGQLNRDLEQAFAELTEEGEENA------5Bacteroides_fragilis_NCTC_9343/1585/1-585 AVTPMERQEAILNMVIDICHTEFEF-DNFNEVMDYFKK--MINICKQMNY SKFK------SEQYEGFQKQLEELVAERSVK------96Sulfolobus_solfataricus_P2/1592/1-592 AFSSPQKQARIMRLIYLFNTYASKL-VERGIPTKKIVDSMG-QLLPEIIR SKAAIKNDE-LNRYDELERKLITVFENLEKEAGT------75Anaeromyxobacter_dehalogenans_2CP1/1579/1-579 ATCPPAKAFEMLRLFLEWHRRAGAA-VGAGVPLRSILD--T-GLGARLLR LAQLPAAEV-PGAAAALRADLSEALARLEAE------235Thermococcus_kodakarensis_KOD1/1585/1-585 TYCPPKKQVTMMRVILNFYEKTMQA-VDRGVPVDEIAK--L-PVREKIGR MKFEPD----VEKVRALIDETNQQFEELFKKYGA------92Nitrosopumilus_maritimus_SCM1/1592/1-592 TYCSPEKQYKLMKLLVDFYKKGQQA-IKEGTPLADIRA--M-KSITTLLK ARMDVKDDE-MPKLDQLDADMQEEFKSITGVKVSN------190Finegoldia_magna_ATCC_29328/1587/1-587 TFCSLDKQAKMLDLILTFHKESLEA-LQEGAYVSEISK--L-PVREKITR AKNIPEDEL-QR-FDEIKQEIKSQITDLVNGGER------999Nanosalinarum_sp._J07AB56/1584/1-584 QYSSPQKTFDMLEVILEYADHAYEA-LDEGALVEDIVS--L-NSRAEIGR IKTAEDH---REKLEEVREQMQDEFGEVAEQ------50Nautilia_profundicola_AmH/1571/1-571 AFCTPEKQIEMAKTVVLIHDLWRKAFLTKQIPVDILKN--Q-PVISEFIR AKYEIKNEE-YEKYIDLRKKIESYYNEFIKNYGE------82Pyrobaculum_aerophilum_str._IM2/1593/1-593 TPSAPIKQFLLMKAIYTYYEEGLKA-IESGVPASVLRE--L-ETVKRLPR LRMEVTNDVAKEELTKFIESLVAEIRATTNRRG------68Allochromatium_vinosum_DSM_180/1598/1-598 TFCSPAKQYALLDLAITLYKRGEAL-VKLGVPVTELQR--L-PLLAKMRR IKSMYSSEQ-LEQIQAFRQEVDQAMEAIRIEYAKQGEAV------89Staphylothermus_marinus_F1/1592/1-592 TYCPPRKAVLMMKAIMLFYELGLEA-IKRGISMEKIRE--L-KSRVKIAR MKEIPNIDF-EDAFKSLFEDIRRDFESLMKEAETIAEL------184Clostridium_botulinum_A_str._ATCC_3502/1592/1-592 TYASLGKQYKMLKLVLFFYDEAQRA-LNAGVYLKELLD--L-EVRDKIAR AKYISEENI-EN-IDAIFNELSEVIDQLISKGGIMNA------205Ferroglobus_placidus_DSM_10642/1573/1-573 TYCDLKKQYDLLKAIKQVADMFYKA-LEAGKLVEEIIN--V-PGKDEFAR AKFEENY---KEALEAAMKKMKEALGV------87Ignisphaera_aggregans_DSM_17230/1591/1-591 AYSPPEKGHMIMKAIMTFYRYAREA-ISKGIPVSRIRE--L-KSRTLIAR TKHHTIDELKTKVFTEIMNTIEQEFKTLLSGE------206Archaeoglobus_fulgidus_DSM_4304/1581/1-581 TYCSVQKQYDMLKAIKQINDWFYQA-LEAGKTIDEIAG--V-EGLEEFAR AKFEEDY---KPAMEAALEKIRKNLLGE------108Coprothermobacter_proteolyticus_DSM_5265/1589/1-589 SYCSLRKQYVILSSILLVHEIILDR-IRKGEDYGSLIA--N-PIREHISQ LRWLKEEEV--PDWKQMQDSILKSFSEEVKAHD------91Thermosphaera_aggregans_DSM_11486/1584/1-584 AFSPPLKTLYMAKVISLFHDYAFDA-LRKGVALEKILT--M-KVKEKIAR MKDIPVFDY-EKAFQEIMDDIKKEFELLLQEVG------246Methanococcus_maripaludis_C6/1586/1-586 SYCSPIKQYHMLKIIMTFYKKGLDA-VAKGADPAGISA--V-TVKGDIAR MKYLVEDEFVNTKVPEIINKMESELGALIK------135Streptococcus_pneumoniae_CGSP14/1599/1-599 TFTSFAKQEAMLSNILTFADQANHA-LELGSYFTEIMEGTV-AVRDRMAR SKYVSEDRL-DE-IKIISNEITHQIHLILETGGL------125Dictyoglomus_turgidum_DSM_6724/1589/1-589 TYTSPYKQYLMLKNIMTFHSIAQSL-IEEGYPLEDILR--L-PEREKIAR MKFVPEDRI-NE-LEALLMEIKKRLEGIKEGTREYA------11Halomonas_elongata_DSM_2581/1627/1-627 ASTSLNRQKAAMALLTRIVDAPLSF-GGKQDIRDVFTR--LTDLFRNLNY AEFE------SDNYWKIRHSIDELLKQYAREASRTAAYPASTP----- 159Clostridium_difficile_630/1592/1-592 TYTSLHKQYRMMGLILKFYKSAQDA-IKQNVTINDIIK--L-SVLEKIGR AKYVEEGDI-DAAYDSIEDEIDNELADLIKKRGAEEL------800Nanosalina/1586/1-586 QYSSPQKTFEVLQLILDWGDSAYEA-LEDGAVVEDIIS--L-SCKNEISK VKTSEDY---EQKIEEVREQMQEEFTELDK------996Pyrococcus_sp._NA2/1588/1-588 TYCPPEKQVTMMRVLLNFYHKTMEA-INRGIPLEEIAK--L-PVREEIGR MKFERD----IEKIRELIDKTNEQFEELFKKYGA------219Methanoculleus_marisnigri_JR1/1584/1-584 TFCPMSKQYDMMKAIKHYADLARTA-QTGGATPQQVIG--I-RSKNELPQ IKFIRDY---EPELAKIMKDMEAEFDAMRAV------65Finegoldia_magna_ATCC_29328/1588/1-588 TYVPLKKQVEMLKTIKLLYEEGRKA-IKEQIPISQVKN--A-DLYSKVIA MKNTISNED-LSGIKDLNDEIKEFYSDLISKYEK------38Borrelia_recurrentis_A1/1576/1-576 AAVSFERQNYMFNILYKILQSDFKF-ENKLEARSFIND--LRQNILDMNL APFK------QEKFNKLEINLKNLLRSKKLDF------32Desulfohalobium_retbaense_DSM_5692/1589/1-589 GATTLKRQSYVFDRLHSFLQTSMTF-ADKDAARSFFQK--LTQATIDWNR LAMD------SDDFHHQEKRLEQLVADVTDYA------163Mahella_australiensis_501_BON/1587/1-587 TYTSMNKQYRMLKIIMDYYKRGQQA-LDEGVELDAITN--L-PVRERIDR AKYIKEENM-NQ-FDDIEKQMAEQFAQLLESSKA------129Eubacterium_limosum_KIST612/1601/1-601 TYSSLEKQYAMLSMILAWYEKGVAA-LELNVPFAKIIT--M-DIREKIAR MKYVPEDQK-EERFPAIAAEMEAEFLALMEGSGDDE------69Sideroxydans_lithotrophicus_ES1/1599/1-599 SYCSPKKQFALLDQMLAIYQDGLKL-LEQGVPVQELLR--M-PVLAQAQR LKSSYGNDK-LDGLREFAEKTRSEFHRVSDEYAQHGKATTTDGDRT-- 214Methanocella_paludicola_SANAE/1579/1-579 TFCSLKKQFMMMNEIMAFGRLAKRA-LAAGVPMPKILG--L-KSKTDLAK VKFEADF---EKYLGNVDNEMKAEFKALEAA------70Nitrosococcus_halophilus_Nc4/1593/1-593 SFCTPEKQFALLELMLQLYHRGTEL-LERGVPVQELLG--L-PILARAKR CKSDYDNTK-VEKLRDFAKTIREEFDRLGMEYAEAGKHSE------229Thermococcus_sibiricus_MM_739/1585/1-585 TYCLPKKQVTMMRVILNFYRHTMRA-IDAGIPVEEIAK--L-PVREEIGR MKYNPN----IEEIAVLMEKTKEQFEELFKKYGE------175Clostridium_perfringens_SM101/1591/1-591 TYTSLNKQYKMLNLILSFRHEAEKA-LEAGVYLDKVLK--L-PVRDRIAR SKYISEEEI-SK-MDDILVELKSEMNKLISEGGVLNA------179Clostridium_tetani_E88/1592/1-592 TYAPLNKQYRMLKAVLQFGDEARKA-LESGVYLKDILN--L-PVRDKIAR AKYIDEKDI-LS-IDEISKELTKDIEDLISKGGILDA------107Aeropyrum_pernix_K1/1597/1-597 AFATPQKQFRLLKMIMDIHRKSLEL-IEKGVTTQQILQALG-RLYIDIVK AKFTIPNDQ-LEKIDEIRDRALKALDELVSG------105Ignicoccus_hospitalis_KIN4/I/1596/1-596 AFCSPKKQFLMMKIIIDFYRKAKEL-VNAGVPVATIREAVK-EEVAELIR SRFTVRNEE-LEKLEDLYARFMEKLSSLSP------301Thetis_Sea/1-587/1-751 TYCSVEKQLRMFEIVLDFYDETVGA-VEEGGFGRGGHS--A-PVRRNIAR MKTIPDEKF-MEQADEIEDEIDDQIEEVMEEGD------31Desulfarculus_baarsii_DSM_2075/1581/1-581 AYNTMERQVYTFKKVLELMDKEIKV-TDKDEARDLFFR--LAALFKNMNA SAMQ------SEEFAKFESQINDFIGS------158Clostridium_phytofermentans_ISDg/1589/1-589 TYTTLEKQFRMMELVLYFYDASLAA-LKKGASIDALVK--V-PVREQIGR FKYEKEENL-QEVYGAIKHQIMEQAEQLVGREDA------193Halobacterium_salinarum_R1/1585/1-585 RYCPPEKTYAILSGIKTLHEESFEA-LDAGVPVEEITS--I-DAAPRLNR LGTTPDDEH-EAEVAEIKQQITEQLRELY------46Spirochaeta_thermophila_DSM_6192/1591/1-591 AAVGVERQRHTFDIVFDILSALYSF-TSKEEARRFFAL--LTQKFKDYNT SEWD------SADFRRLESEIRAMVEEKTTGRDEEAEYILSRKVESHA 196Halorhabdus_utahensis_DSM_12940/1587/1-587 TYCEPEKTYRMMEAIRTFNDEAFDA-LDAGVPVDEITD--I-DAAPRLNR IGTTEEY---DEFVAELEDDIESQLRGTYQ------12Legionella_longbeachae_NSW150/1603/1-603 VSVPINRQKEFFNLLHDIITKDFEF-TEKEQARNFFVK--LTGLLKNLNY SPLN------SDAFINYYNNIIQLTQL------236Methanocaldococcus_infernus_ME/1589/1-589 TYCPPMKQYLMLKIIMTFYEEALKA-VERGVEPSKILK--V-SVKQDIAR MKYMPHDEFINVKSKEILEKIKKELSSLT------300Thermococcus_litoralis_DSM_5473]/1585/1-585 TYCPPKKQITMMKVILNFYHYTMRA-IDMGVPVEEIAK--L-PVREEIGR MKYNPN----VEEIASLMKKTEEQFEELFKKYGE------231Pyrococcus_horikoshii_OT3/1964/1-588 TYCPPEKQVTMMRVLLNFYDKTMEA-INRGVPLEEIAK--L-PVREEIGR MKFERD----VSKIRSLIDKTNEQFEELFKKYGA------73Anaeromyxobacter_sp._Fw1095/1578/1-578 ASCPPRKALEMLRLHLELHGRAVNA-IAGGVPLHSILE--A-GLGARLLR LGSLPAEAI-ARGAEALRAELEGLLARLEAE------194Halalkalicoccus_jeotgali_B3/1587/1-587 QFSPPEKSYLIWKAIKTFNDEAFAA-LEAGVPVEEITA--I-DAAPRLNR IGTQEDY---EAYVEDLQSDIEDQLREKY------202Haloterrigena_turkmenica_DSM_5511/1587/1-587 TYCEPEKTYRMLEAIKTFNDEAFNA-LEAGVPVEEITD--V-DAAPRLNR MGTAEEW---ESFIEEIENDLQEQLRALY------997Methanothermus_fervidus_DSM_2088/1585/1-585 TYCPPEKQFKMLETILLFNRETKKA-LKKGAPIDKLTQ--L-PVKEDIAR MKYIPPDEF-EEKVKEIQDKIIKQCEEVLK------802Parvarchaeum/1583/1-583 TYASVNKQYKMLKLINQVFDAEKAA-LDRNVPIEKIQS--V-QIKPKIAR LRYTSEEEL-DGKLKEIEKDIEE-IKKINAEK------208Methanosarcina_barkeri_str._Fusaro/1578/1-578 AYSPFAQQYRILKAIMKWGDAAMEA-LKSGVPVPEILK--L-ESKNVLAK VKYEEKF---DESMNAVLTQMDKEFASLRGR------Ferroplasma_sp._Type_II QFCSLNKQIVMMKLIMHYSNEIEKT-IDQGFSMSQIEN--I-PSKEKISR IKEVRDPDL-EKYFNETVTSIDSDLKRIRGEK------84Caldivirga_maquilingensis_IC167/1595/1-595 TPSAPEKQYWLLRLMITYYRVGSEA-INAGVPANAIRE--L-DSVRRLIR LKMEVKSSD-YKVLADYERKLIDDIRGLMAKFSK------197Haloquadratum_walsbyi_DSM_16790/1585/1-585 TYCSPEKTYLTVTAIHQFNDEAFEA-LDAGVPVDEIIS--I-DAAPRLNR IGVQDDY---EEYIATLKEDIATQLQELY------232Pyrococcus_abyssi_GE5/11017/1-588 TYCPPEKQVTMMRVLLNFYDKTMEA-ISRGVPLEEIAK--L-PVREEIGR MKFEPD----VGKIKALIDKTNEQFEELFKKYGA------200Halomicrobium_mukohataei_DSM_12286/1586/1-586 TYCEPEKTYRILDAIKTYNDEAFDA-LDAGVPVEELTD--L-ETVPRLNR IGVQEEY---NEYIDELEADIESEIQELY------143Streptococcus_sanguinis_SK36/1623/1-623 TYTSFKKQVALLSNILTFDAEANRA-LELGAYFREIMEGTV-ELRDRIAR SKFIHEDQL-GK-IQALSQTIEETLHQILAQGGLDNERH------30Waddlia_chondrophila_WSU_861044/1596/1-596 AYCPLPRQIAQFSLINKVFDTEFDF-KTHDEAREFFLS--LQNQVKNLNY MTFD------SGEYRSAYEKLVELIQSREKQEVLA------45Spirochaeta_smaragdinae_DSM_11293/1588/1-588 AAVGVERQTYVFNLLFEIIGSNYDL-SQKDEARTYFNQ--LRQRFLDFNG SEWK------SDEFVAQEKMIRQMFDEKKTGFQEDAKRLLKQA----- 131Thermanaerovibrio_acidaminovorans_DSM_6589/1569/1-569 TYASMNKQFKMLRNILTFHHLAMEA-LQRGALLKDVLE--L-PIREEIAR MRYLDEKEI-SK-LDELEDRIKQVLGQLSASGGDEDVA------42Treponema_pallidum_subsp._pallidum_str._Nichols/1589/1-589 SAVPVARQKHCYAIVMRVLGSVLAF-ESKDDARAYFSK--LGHMFIDYNC CAWN------SEAFVEKEKEIRAFLQGESTKIDSEAEGIIRGME---- 77Thermofilum_pendens_Hrk_5/1601/1-601 VFCKPEKQYWLLKMMMDFFDKSYEL-IRKRVSIEEILR--M-PEIYEMMR VKEDERG---LQAVKELYERVMAKLDEIAQRHGVTLAVEAEEVVA--- 110Thermus_thermophilus_HB27/1578/1-578 AYCSMRKAYGIMKMILAFYKEAEAA-IKRGVSIDEILQ--L-PVVERIGR ARYVSEEEF-PAYFEEAMKEIQGAFKALA------203Natrialba_magadii_ATCC_43099/1587/1-587 TYCDPKKTYRMLLAIKTFNDEAFDA-LEAGVPVEEITD--V-DATPRLNR MNTSDEW---NEFIDELESELEEQIRSLY------93Sulfolobus_acidocaldarius_DSM_639/1592/1-592 AFSSPQKQAKIMRLIYDFYTNASQL-LDKGLTLKKILEKVG-SFEPDIVR VKYTVKNDE-LNKIDELDNKLKEAFDSLLKEVA------35Borrelia_burgdorferi_B31/1575/1-575 AAVSSERQNYMFDIVYNILKTNFEF-SDKLQARDFINE--LRQNLLDMNL SSFK------DHKFNKLEHALGELINFKKVI------55Spirochaeta_smaragdinae_DSM_11293/1592/1-592 RYSTVEKQARMLSLIITYWRKGREA-IKRGATLSKVKR--L-KAVQELVK MKFTIPNED-TKEFDKLGARLERAFDQMESMYAAR------29Candidatus_Protochlamydia_amoebophila_UWE25/1593/1-593 AYCPLKRQIALFQLINQIFDTTFDF-HTHDQAREFFLD--LQNRIKNMNF ISFD------TEQYRKVFAEIKSIIEQQSRK------198Haloferax_volcanii_DS2/1586/1-586 TYCSPEKTYGILTAIHAFNDEAFKA-LEAGVPVEEIQA--I-EAAPRLNR IGVQEDW---EAYIEDLKAEITEQLRELY------302Thermoplasmatales_archaeon/1-575 TYCSLKKQFLILKSILQMDQLEKYA-VSKGAAVSDLNS--L-SYREKLSR FKEVKEGDV-EQYFTSLSKLMED------62Halothermothrix_orenii_H_168/1590/1-590 RFATPEKQYWMLKIIMFLYEKARPL-IKNNIPISRVKN--N-ELFSEVIK MKENIPNGD-LDKFKDLMARIEEYYNRLWENYQE------115Truepera_radiovictrix_DSM_17093/1579/1-579 ASCSLSKAYGMLQMIIALNDQARRA-LDAGATVDDVLK--S-PVIERVNR ARYVPEDEF-AAYKEATLKEIAQGFAVAA------16Chlamydophila_pneumoniae_CWL029/1591/1-591 CYCPFERQIELFSLISRIFDAKFVF-DSPDDARSFFLE--LQSKIKTLNG LKFL------SEEYHESKEVIVRLLEKTMVQMA------213Methanohalophilus_mahii_DSM_5219/1576/1-576 TFCPFDRQYKLLKSVSTFGEKATEK-MENGVPITKIME--M-KSKDELAK VKYEEDF---DAALEAIMAKMDEEFTQIGGN------78Candidatus_Korarchaeum_cryptofilum_OPF8/1595/1-595 TYCSPKKQAILLRTLLEFYERAKPL-IEAGVPMSKIAE--M-RSLGLLRR LKFVPEKEF-EAAARDAISQLEAEISSLLAEVR------19Chlamydia_muridarum_Nigg/1591/1-591 CYCPFDRQIELFSLMSHIFSSRFCF-DCPDNARSFFLE--LQSKIKTLNG QKFL------SEDYQKGLEVIYKLLESKMVQTA------52Thermanaerovibrio_acidaminovorans_DSM_6589/1594/1-594 AYSPPSKGFAILDRIIYFHDRCEGL-IKKGVPLSLIKG--H-ESVEAMAH LRELPADD--IRAFDDHKRRLDAHIERVARERTAASTGR------223Methanopyrus_kandleri_AV19/1592/1-592 TYCPPEKQYEMLKTILHFKERAEEA-VDKGVPVDEILK--L-DVIDDIAR MKVIPNEEA-KEKIQEIRKKIDEQFEELIEEAS------189Leptotrichia_buccalis_DSM_1135/1597/1-597 TYTSLEKQDKMLDIVLKFYDEGLRG-LENGAYLNEIIA--M-PVRERIAR AKYLPEAEL-GK-IEEIAKELEKEIDELVNKGGVANA------191Fusobacterium_nucleatum_subsp._nucleatum_ATCC_25586/1382/1-584 TYCSLTKQFKMLKLILSFYDEAQRG-IKEGVYLDEILA--L-PAREKITR AKNISEKEL-DS-FDKIEEEIKEVISKLIAEGGKPNA------4Bacteroides_vulgatus_ATCC_8482/1584/1-584 SVTPMERQEEILNMVIDICHTEFKF-DTFIEVMDYFKK--MINLCKQMNY AKYK------SEQYEDFKKQLQELVAERSV------95Metallosphaera_sedula_DSM_5348/1591/1-591 AFSSPQKQARIMKLIYHYNQYATNA-VERGIPVKKIVDKI--TVVPDIIR SKATIKNNE-LQKYDELENKLKAQFDELLKEAGA------44Treponema_denticola_ATCC_35405/1589/1-589 DAVSVERQQHIYNILIEILGTSFKF-VSKDEARSYFSK--LKLMFIDYNY SPWG------SDAFKSHEDGIKKHIAEKADSLDERAKKLLKQAV---- 90Desulfurococcus_kamchatkensis_1221n/1584/1-584 VFSHPVKTVKMATIIDKFYEKALEA-IKLGVSFEKIRG--M-KIRERIAR MKHIPFGDN-TKEFDSILIEMEEEFKNLREES------57Thermotoga_sp._RQ2/1586/1-586 AFCPPEKQFRILKLIVDTYRSARKF-IEKSIPVGKILP--P-EIVSKLST LKESRNFE---EEIVKTEKIIKDHFEQLEKLYATGTR------803Pyrococcus_yayanosii/1587/1-587 TYCPPKKQVTMMRVLLNFYEKTMQA-IDRGIPIEEIAK--L-PVREEIGR MKFEPN----VEKIAALIDKTNEQFEELFSRYG------228Methanobrevibacter_smithii_ATCC_35061/1584/1-584 TYCPPVKQYNMLKTILLFHKEALAA-VGRGVPIQNIVA--L-PVKEEIGK MKYIPQDEF-AAKCEEIQAAITKQCSEA------13Cyanothece_sp._PCC_8801/1607/1-607 ASAPLQRQKETFTLVYNIVKREYKF-PDKSAVRDYFTR--LTNLFKNLNY AHPD------SNERANYFKQINEFVESMTNLERG------168Thermoanaerobacter_pseudethanolicus_ATCC_33223/1590/1-590 TYSSMEKQYRMLKLIMIFYQEAQKA-LEKGVPFSEIEK--H-PVREKIAR AKYVEESKL-TV-FDEIEKEIKKAMQGLIEGGAADA------26Chlamydophila_felis_Fe/C56/1588/1-588 CYCPFDRQIELFSLMRRIFDAKFSF-DCPDNARSFFLE--LQSKIKTLNG RKFL------SDEYKEGMEVVLRLIETKKI------80Thermoproteus_neutrophilus_V24Sta/1594/1-594 TPSSPIKQFLLMKAIYAYYEEGMKA-IEAGVPAAVLRE--L-ETVKRLPR LRMEVTNDVAKEELTKFIESLVSEIRSVLAARKQ------114Deinococcus_radiodurans_R1/1582/1-582 ASASMPKNYGLMKMFLKFYDEAEAA-LRNGLTIDEIIQ--N-PVIEKLAR ARYTPENEF-MAYGEGVMDELDQTFKAVKA------122Methanocella_paludicola_SANAE/1579/1-579 SFCPLDKQYYMLKAIIAFHNATVHA-INRGVPLKKVMD--L-PLKAEIGR MKEIKEADR----IKSMVDDIGGRIGALEADK------217Methanoplanus_petrolearius_DSM_11571/1582/1-582 TYCSMEKQFDIMKAIKKYADLGYDA-QKSGAPIAAVVG--V-KAKNELAQ IKFIAEY---KPELGKILKQMDEEFAKVKEA------998Thermoplasma_acidophilum_1221B2/1590/1-590 AYCSLKKQYLMLKAIMEIDTYQNKA-LDSGATMDNLAS--L-AVREKLSR MKIVPEAQV-ESYYNDLVEEIHKEYGNFIGEKNAEASL------204Methanosaeta_thermophila_PT/1576/1-576 TFCPYKKQYDLLKAILTYRDYAFDA-LRRGVAVDQIKS--V-PSKDALAK LRMVEDY---EPDLKKVMDQMKAEFEALK------218Methanospirillum_hungatei_JF1/1582/1-582 TYCDMTKQFDILKAIRFYSDQAYAA-LKAGVITSQITG--L-KAKNDLPQ IKYVKEY---KPEIERIVKTMESEFTKLREAA------221Candidatus_Methanosphaerula_palustris_E19c/1588/1-588 TFCSMKKQYDMLKSIKTFSDLSYAA-QTVGVSPQQIIA--V-KSKNELSQ VKFTADY---EPLLEKILKDMEAEFNALRAGA------171Anaerococcus_prevotii_DSM_20548/1585/1-585 TYCSLRKQDKMLATILYNYDKSLEA-LSAGVELEEIEK--L-PVREKITR LKLISEDDL-GN-IEEIRSEIDKQIEELIREA------120Ferroplasma_acidarmanus_fer1/1589/1-589 TYCSIKKQYEMLTIIKTLNEMQEKS-IASGLKLTQTAT--L-PVRMKISR MKEIPENDF-EKFYNDVIKEINNEYDNIMEGVESV------119Thermoplasma_volcanium_GSS1/1776/1-590 SYCSLRKQYLMLKAIMELNSYQSMA-IDHGVTMDNLSS--L-PVREKLSR MKIVPEDQV-ESYYSSIIKEIHKEYTSFIGEKNAEANI------3Brachyspira_murdochii_DSM_12563/1587/1-587 KNCPIERQKELLNQVMKVVEADYRF-DDYSEVGTYFKR--LINAFKQMNY SVYQ------SDDHKKYTAEMESIFAERSITHA------59Anaerococcus_prevotii_DSM_20548/1582/1-582 KYVPLEKQVKMLDIIYNYYQKSKAA-ISGGLSHKAIYD--S-NLINEITQ MKYNIKNDE-LDKFIDLNSKINNHFVSVKEGL------164Clostridium_thermocellum_ATCC_27405/1589/1-589 TYTSLNKQYRMLKLILGFYYSGKKA-LEAGVSIKELFE--L-PVREKIGR AKYTPEDQV-NSHFNEIEKELNEQIEALIAKEVQ------237Methanocaldococcus_vulcanius_M7/1587/1-587 TYCPPMKQYLMLKIIMTFYQEALKA-VERGVEPAKILK--V-SVKQDIAR MKYIPHDEFINVKSKEIMEKIKNELGSLN------124Dictyoglomus_thermophilum_H612/1589/1-589 TYTSPYKQYLMLKNIISFHRIAQSL-IEEGYPLEDILR--L-PEREKIAR MKFVPEDRI-NE-LEALLVEIKQRLEGIKEGTREYA------153Streptococcus_pyogenes_M1_GAS/1591/1-591 TFTSFPKQEAMLTNILTFNEEASKA-LSLGAYFNEIMEGTA-QVRDRIAR SKFIPEENL-EQ-IKGLTQKVTKEIHHVLAKGGI------41Fibrobacter_succinogenes_subsp._succinogenes_S85/1585/1-585 AACSAERQKYVFDKIYTILMTPMTF-TEKDVARTFFLK--LTQATKDWNR VAFD------SQEFKDLESSIFASVKEVSANA------220Candidatus_Methanoregula_boonei_6A8/1588/1-588 TFCDMQKQYDMMKAIRLYSDLANTA-QAAGVSPAQITT--I-KAKNELPQ IKFVKDY---KQPLAKIEKDMDAEFNALRSAA------79Pyrobaculum_islandicum_DSM_4184/1593/1-593 TPSSPIKQYLLMKAIYAYYEEGLKA-IEAGIPASVLRE--L-ETVKRLPR LRMEITNDVAKEELTKFIEMLISEIRTTISKRQ------177Clostridium_botulinum_E3_str._Alaska_E43/1592/1-592 TYTSLKKQYKMLSLILGYRKEAERA-LEAGVYLNDILA--MEDLKDRIAR SKYIHEDDL-EK-MDQIVVDLKNAIDNLINKGGVANA------48Geobacter_uraniireducens_Rf4/1524/1-524 AFSTPEQTVAKIGEILEFHDRAAAR-LKEGVLLDEVLK--S------211Methanohalobium_evestigatum_Z7303/1578/1-578 RYCPFYKQYKLVQAIHKFSETANSK-LEAGYSINDIMA--M-ESKDELAK VRYEEDF---DGALNKVLSKMDDEFSQIGG------230Pyrococcus_furiosus_DSM_3638/11013/1-588 TYCPPQKQVTMMRVLMTFYERTMDA-ISRGVPLEEIAK--L-PVREEIGR MKFEPD----IEKIRALIDKTNEQFDELLKKYGA------47Stackebrandtia_nassauensis_DSM_44728/1575/1-575 AHCTTAKTVALAEAVIAVIDAAQTA-VTTGVPAADVEG----ADYSPLVR AREESGPDD-VEGVRARRDVVLERLKELAS------199Haloarcula_marismortui_ATCC_43049/1586/1-586 TFCEPEKTYRIMDAAKTYNDAAFEA-LDAGVPVEEITD--I-DAAPRLNR IGVQEDY---NEYIDDLEDDIESQLRELY------227Methanobrevibacter_ruminantium_M1/1584/1-584 TYCSPIKQAGMLETILKFHEAASAA-VTRGADVKEIAA--L-TVKEDIAR MKYIPEDEF-EARVKEIQEKVVTQCAEV------10Parabacteroides_distasonis_ATCC_8503/1585/1-585 AVTPLRRQEFMLDRVVNVCRSEFKF-DNFLEVMDYFKK--MINIFKQMNY SEYE------SDQFKKFNTELDELLKERIEN------195Halorubrum_lacusprofundi_ATCC_49239/1591/1-591 TFCPPEKTYLMLTTIETFNDEAFDA-LEAGVPVEDLVD--T-EAAPGINR IGVQEDY---EEYIEELKAEISEELRSLY------64Thermosediminibacter_oceani_DSM_16646/1587/1-587 SYVPLDRQYTMLKVIDRLYERALSS-VKKGIPIAKVKN--E-KIFNDVIM MKYTLN------FDELIKQIDEYFDSLESMYKEGVFYEG------144Streptococcus_gallolyticus_UCN34/1591/1-591 TFTSFAKQEAMLNNILNFADEAKKA-LELGAYFTEIMDGTV-EVRDRMAR SKYIAEENI-DQ-IKALSEQAQEAIHQVLAKGGI------165Thermosediminibacter_oceani_DSM_16646/1590/1-590 TYSSMNKQYRMLKLILMFYEEAQKA-LEKGALFSEIEK--H-PVRERIAR AKFIEESNL-AE-FDEIEKEIKKAMQDLAEGGGADA------172Clostridium_cellulovorans_743B/1591/1-591 AYSSLNKQFIMMQIILAFQDEAQRA-LKAGVYLDKILK--L-PVREKIAR SKYISEDEI-NK-ISDIKNQLVSEIDKLIEEGGIM------201Natronomonas_pharaonis_DSM_2160/1585/1-585 TYCEPEKTYQMLTAIQHFNDEAFEA-LDAGVPVEEIID--V-DAVPQLNR MNTQEDY---EEYIEELKGDLTDQLRELY------178Clostridium_novyi_NT/1591/1-591 TYSSLEKQYKMLKLVLSFQDEAERA-LKAGVYLEKITS--MVELRDKIAR AKFIPEEEM-GR-IDEIGEELRREIDKLIAEEGVINA------141Streptococcus_mitis_B6/1591/1-591 TFTSFAKQEAMLSNILTFADQANHA-LELGSYFTEIMEGTV-AVRDRMAR SKYVSEDRL-DE-IKIISNEITHQIHLILETGGL------109Methanospirillum_hungatei_JF1/1588/1-588 TFTGPKKQYRMLKAIFTFFSQAERA-LETGISVSRLRG--L-PVIESFAR MGTASPEEE-DPLFESIARDTDA-IRDLKEVL------49Aminobacterium_colombiense_DSM_12261/1588/1-588 ASCSMKKQFILLKLLYDLDNLMRQR-IESGLLYDQVAD--Y-PLRVELLR LREKPEIEL-EQQGNQWLKNFDDKLESLVVIPE------118Thermoplasma_acidophilum_DSM_1728/1764/1-590 AYCSLKKQYLMLKAIMEIDTYQNKA-LDSGATMDNLAS--L-AVREKLSR MKIVPEAQV-ESYYNDLVEEIHKEYGNFIGEKNAEASL------94Sulfolobus_tokodaii_str._7/1595/1-595 AFSSPQKQVRIMRLIYIFYNQSQDL-ISKGVPLKKILDKVG-PIEPEIIR IKYTIKNDE-LNKIDEIENKLKATFDSLLKEVS------102Sulfolobus_islandicus_M.16.27/1592/1-592 AFSSPQKQARVMRLIYLFNTHASRL-VERGIPTKKIVDSMG-QLLPEIIR SKAAIKNDE-LNKYDELERKLISVFENLEKEAGT------53Treponema_pallidum_subsp._pallidum_str._Nichols/1605/1-605 VFSCPEKQVQILRTIVDFHERAVVL-LRAGISLSALSQ--L-SCRELIVR MKTTYGNED-VHKMQKVYDTMCTEFDQLSVCAAARTQGGEKVE----- 106Hyperthermus_butylicus_DSM_5456/1601/1-601 AFATPQKQWKQLETIIDFYHAALEA-IKRGVTVKEIREKLA-PKIRELIL ARYNVPNDQ-LEKLDKLKQELLQALQELIEAKAGRASAAT------104Acidilobus_saccharovorans_34515/1601/1-601 TFTTVQKQMALMRMFVEFYRAASDA-VRRNVPVNKIKDSLG-RMYSYMMR ARFTIPNDK-IDEIDKMTRDIVNIINNIGGGQA------58Acholeplasma_laidlawii_PG8A/1583/1-583 TFVPLHKQLNMIETILYLYDSSLRF-IEKGKALSLILK--S-GIFDQVIQ MKYEIGNEP-TDQFDTLKHLIDQTIDSIF------130Aminobacterium_colombiense_DSM_12261/1598/1-598 TYASMEKQFKMLKNILIFHHLGMDA-LKRGASMSEVFN--L-PVREKIAR MRYIDEKEI-SQ-LDNLDGDMKAEINALIPVGGDSDAA------243Methanococcus_vannielii_SB/1586/1-586 SYCSPKKQYNMLKVIMTFYKKALDA-VAKGADPAKLSA--V-SVKGDIAR MKYMPEEEFIKTKVPEMIKKMESELGALIK------207Archaeoglobus_profundus_DSM_5631/1580/1-580 TYCPLEKQYDMLKGIKMINDWMFQA-LERGKPVEEIIA--V-KGKEKFAR VKFEKDY---KPLLQEALEEIRKNLLGE------999Ferroplasma_acidarmanus/1589/1-589 TYCSIKKQYEMLMIIKTLNEMQEKA-IDAGLKVIQTAT--L-PVRMKISR MKEITENDF-ERFYNEVIKEINNEYDNIMEGVENV------116Meiothermus_ruber_DSM_1279/1578/1-578 ASCSMAKAYGIMQMILAAYKQGELA-LAKGATIADFLN--D-PVIEKIGR ARYVPEEEF-PAYKAEFDQMIKTAFLGAVKA------123Methanospirillum_hungatei_JF1/1584/1-584 SFCPLEKQYWMLKIIIWYYDAIRAA-MRRNVPLRQLLS--I-PARSEIAR MKEQRDLDR----LKRLSDIVWEQTENLEVQTCSTAA------226Methanothermobacter_thermautotrophicus_str._Delta_H/1584/1-584 TYCSPSKQFEMLRTIIMFHRNATAA-LEKGAPAADIIS--L-PVKEDIGR MKYIPEEEF-PARIKEIQERIVKECSEV------241Methanococcus_aeolicus_Nankai3/1586/1-586 SYCSPMKQYTMLKTIMTFYSKAILA-VEKGADPADIAK--V-SVKQDVAK MKYTPEEEFLNKLAPAIVEKISKELDALV------234Thermococcus_gammatolerans_EJ3/1585/1-585 TYCPPKKQVTMMRVILNFYNRTMEA-VDRGVPVDEIAR--L-PVREKIGR MKFEPD----VEKIRALIDETNAQFEELFKKYGV------224Methanosphaera_stadtmanae_DSM_3091/1585/1-585 TYCSPSKQYNMLKTILLYNTTAQSA-LADGADINKLVN--L-DVRVDLGK MKYIPEDEF-EAKVEDIRNRITKECNEAGQ------233Thermococcus_onnurineus_NA1/1585/1-585 TYCPPKKQVTMMRVILNFYERTMEA-VDRGVPVDEIAK--L-PVREKIGR MKFEPE----IEKVAALIDETNAQFEELFKKYGA------66Eubacterium_limosum_KIST612/1595/1-595 TYVPLEKQYRMLKIMDYLYEKGLVG-LKQGIPTSVLYN--E-KIFDDVIK MKYNIPNDD-FSGIDVLRQDIDEYYDDLMRRYQD------188Streptobacillus_moniliformis_DSM_12112/1588/1-588 TYTSLDKQNKMLNMVLHFYNQAQKA-LKKNVYVNDILE--L-EVREKIAR AKYLSEEHI-YK-IDEINKEVEEKINELISRQGEVM------140Streptococcus_pneumoniae_TIGR4/1591/1-591 TFTSFAKQEAMLSNILTFADQANHA-LELGSYFTEIMEGTV-AVRDRMAR SKYVSEDRL-DE-IKIISNEITHQIHLILETGGL------60Clostridium_phytofermentans_ISDg/1588/1-588 TYVPLPKQMKMMEVILYLYEKAKSL-VEMNMPMSLLRE--N-PIFDHVIS MKYDVGNEE-LAKFDTYKTEIDKFYQHFIETNA------Alignment: /Users/jpgogarten/Dropbox/supplementary material for upload/sate_alignment_of_Extein_and_Intein_sequences(withoutHE).fasta 6 Seaview [blocks=130 fontsize=7 LETTER-landscape] on Fri Jul 19 09:49:28 2013 1 Pyrococcus_furiosus_DSM_3638 M-----PAKGRIIRVTGPLVIADGMKGAKMYEVVRVGELGLIGEIIRLEGDKAVIQVYEETAGLKPGEPVEGTGSSLSVELGPGLLTSIYDGIQRPLEVLREKSGHFIARGISAPALPRDKKWHFTPK-V Pyrococcus_abyssi_GE5 M-----VAKGRIIRVTGPLVVADGMKGAKMYEVVRVGELGLIGEIIRLEGDKAVIQVYEETAGVRPGEPVIGTGSSLSVELGPGLLTSIYDGIQRPLEVIREKTGDFIARGVTAPALPRDKKWHFIPK-V Pyrococcus_horikoshii_OT3 M-----VAKGRIIRVTGPLVVADGMKGAKMYEVVRVGELGLIGEIIRLEGDKAVIQVYEETAGVRPGEPVVGTGASLSVELGPGLLTSIYDGIQRPLEVIREKTGDFIARGVTAPALPRDKKWHFIPK-A Pyrococcus_sp._NA2 M-----PVKGEIIRVTGPLVVAKGMKGAKMYEVVRVGELGLIGEIIRLEGDKAVIQVYEETAGVRPGEPVIGTGSSLSVELGPGLLTSIYDGIQRPLEVIREKTGDFITRGVTAPALPRDKKWHFIPK-V Thermococcus_litoralis_DSM_5473 M------GRIIRVTGPLVVADEMRGSRMYEVVRVGELGLIGEIIRLEGDKAVIQVYEETAGVKPGEPVVGTGASLSVELGPGLLTSIYDGIQRPLEILREKSGDFIGRGITAPALPRDKKWHFTSK-V Thermoplasma_acidophilum_DSM_1728 M------GKIIRISGPVVVAEDVEDAKMYDVVKVGEMGLIGEIIKIEGNRSTIQVYEDTAGIRPDEKVENTRRPLSVELGPGILKSIYDGIQRPLDVIKITSGDFIARGLNPPALDRQKKWEFVPA-V Thermoplasma_volcanium_GSS1 M------GKIVRISGPVVVAEDIENAKMYDVVKVGEMGLIGEIIRIEGNRSTIQVYEDTAGIRPDEKVENTMRPLSVELGPGLLKSIYDGIQRPLDVIKETSGDFIARGLNPPPLDRKKEWDFVPA-V Thermoplasma_acidophilum M------GKIIRISGPVVVAEDVEDAKMYDVVKVGEMGLIGEIIKIEGNRSTIQVYEDTAGIRPDEKVENTRRPLSVELGPGILKSIYDGIQRPLDVIKITSGDFIARGLNPPALDRQKKWEFVPA-V Thermoplasmatales_archaeon_I-plasma, M------GKILRVAGPVVIGEDVEGAKMYDVVRVGEMGLVGEIIRMEGDKATIQVYEDTSGIKPGDKVESTLRPLSVLLAPGVLKSIYDGIQRPLDVIRAQTGDFIKRGLYPDPVDLKKKWKFVPS-V Methanothermus_fervidus_DSM_2088 M-----TGEKRIIRVSGPVVVAEGMKGSQMYEMVRVGEEKLIGEIIELEGDKATIQVYEDTTGLKPGEKVETTGGPLSVELGPGVLGEIFDGIQRPLDKIKAMTGEFIRRGVDVPSLPREKKWKFKPT-V Mahella_australiensis_501_BON M------SQGSIVKVSGPLVVAQGMGDANMYDVVRVSGMGLIGEIVEIHGDMAYIQVYEETSGLGPGEPVESTGQPLSVELGPGLIEAIYDGIQRPLNAIREQAGDRIARGVRAPSLDRDKKWHFTPV-I Candidatus_Nanosalinarum_sp._J07AB56 MTQETIESEGEIYKITGPVVVAEDL-DCQMNDVVYVGGEELLGEVIQIEGGQAYIQVYEETTGVSPGEPVKNTGEPLSVELGPGLLGSIYDGLQRPLPELEEQMGSFIQRGEDAPGINPEEEYSFEPA-V Thetis_Sea_contig_00086 M------GRIIRVAGPVVTADGMRGTEMHEITRVGDEELIGEVIELEEDKATIQVYEETSGLQPGEKVDRTDEPLSVELGPGMIGTVFDGIQRPLPKIREESGSFIDRGVQTNPLPRDKEWTFEPADL Ferroplasma_sp._Type_II M-----KDKGTIYSISGPVVIATDL-DGKMFDVVRVGNMGLVGEVIKIVGNKFTIQVYEDTSGLKPGEPVRSTGKPLSVELGPGLLKSIYDGIQRPLDIIQSETGDFIVRGATAPSLDEEKEWNFTPI-L Picrophilus_torridus_DSM_9790 M------SGSIYSVSGPVVIAQDIENAKMFDVVRVGELGLIGEIIRISGNKATIQVYEDTSGLRPGEKVYSTGKPLSVELGPGLLSSIYDGIQRPLDVIRAKTGDFIAKGVNIPPLNEEKLWDFKPL-V

131 Extein Intein boundary Pyrococcus_furiosus_DSM_3638 KVGDKVVGGDIIGEVPETSIIVHKIMVPPGI-EGEIVEIADEGEYTIEEVIAKVKTPSGEIKELKMYQRWPVRVKRPYKEKLPPEVPLVTGQRVIDTFFPQAKGGTAAIPGPFGSGKCVDGDTLILTKEF Pyrococcus_abyssi_GE5 KVGDKVVGGDIIGEVPETSIITHKIMVPPGI-EGEIVEIAEEGEYTIEEVIAKVKTPSGEIKELKMYQRWPVRVKRPYKEKLPPEVPLITGQRVIDTFFPQAKGGTAAIPGPFGSGKCVDGDTLVLTKEF Pyrococcus_horikoshii_OT3 KVGDKVVGGDIIGEVPETSIIVHKIMVPPGI-EGEIVEIAEEGDYTIEEVIAKVKTPSGEIKELKMYQRWPVRVKRPYKEKLPPEVPLITGQRVIDTFFPQAKGGTAAIPGPFGSGKCVDGDTLVLTKEF Pyrococcus_sp._NA2 KVGDKVVGGDIIGEVPETSIIVHKIMVPPGI-EGEIVEIAEEGDYTIEEVIAKVKTPSGEIKELKMYQRWPVRVKRPYKEKLPPEVPLITGQRVIDTFFPQAKGGTAAIPGPFGSGKCVDGDTLVLTKEF Thermococcus_litoralis_DSM_5473 KVGDKVVSGDIIGVVPETSIIEHKIMVPPGI-EGEILEIAEEGDYTIEEVIAKVKTPDGEIKELKMYQRWPVRVKRPYKEKLPPEVPLITGQRTIDTFFPQAKGGTAAIPGPFGSGKCVDGNTLVLTEEF Thermoplasma_acidophilum_DSM_1728 KKGETVFPGQILGTVQETSLITHRIMVPEGI-SGKVTMI-ADGEHRVEDVIATVSGNGKS-YDIQMMTTWPVRKARRVQRKLPPEIPLVTGQRVIDALFPVAKGGTAAVPGPFGSGKCVSGDTPVLL-DA Thermoplasma_volcanium_GSS1 KKNDIVYPGQVIGTVQETSLITHRIIVPDGV-SGKIKSI-YEGKRTVEDVVCTISTEHGD-VDVNLMTTWPVRKARRVVRKLPPEIPLVTGQRVIDALFPVAKGGTAAVPGPFGSGKCVSGETPVYLADG Thermoplasma_acidophilum KKGETVFPGQILGTVQETSLITHRIMVPEGI-SGKVTMI-ADGEHRVEDVIATVSGNGKS-YDIQMMTTWPVRKARRVQRKLLSRDPLVTAQSGNRCAFPVAEAANCRVPGPFGSGKCVSGDTPVLL-DA Thermoplasmatales_archaeon_I-plasma, KMGESVTAGDVIGTVQETSIIEHRIMVPPGM-EGVIADI-SEGQFGPVETVAHLSSKSGS-REVSMGQYWPVRSPRPVVRKLPPEIPLITGQRVIDTFFPVAKGGTVAVPGPFGSGKCVTGDTPVLLRDG Methanothermus_fervidus_DSM_2088 DVGDEVVGGDIIGEVQETKSIVHKIMVPPNI-SGSIENI-EEGKFKVDEKIAEIKTDDSV-EELKMMQKWPVRKSRPYKKKLDPDVPLITGQRVQDTFFPIAKGGTAAIPGPFGSGKCVSGDTPILLGDG Mahella_australiensis_501_BON EKGAHVTAGDIIGVVQETPIVEHRIMVPYGI-EGTVEEI-YEGDFTVEDTVARVAVKGGI-KEITMMQHWPVRRGRPYKRKLPPDRPLITGQRVIDTLFPVAKGGTACIPGPFGSGKCVSGDTPIVLADG Candidatus_Nanosalinarum_sp._J07AB56 EEGDHVEPGDVIGTVQ-VSYGEHKVLLPPHSDGGEVEEV-REGNYTVTDHVAELDTG----EKVSMRQEVPIRDQRPAEEQLPPEVPLITGQRVFDGLFPLAKGGTAAVPGGFGTGKCVVGDTPVALADG Thetis_Sea_contig_00086 DEGEEVEPGDVLGKVDETTLVEHKIMVPPGV-SGELKELVGRAEYTITDMIATIQTEDGE-EEVQMLQEWPVREPRPYDDKLAPEIPLISGQRIMDTFFPVAKGGTASIPGGFGTGKCVTGETPVLLSDG Ferroplasma_sp._Type_II KDGDEVEQGYILGTVQETDIIVHKIMVPYGV-NGIIKNI-KSGKFRVSDTVCTIDSGNVK-HEIKLKQIWPVRQARKVFLKFAPEIPLITGQRVIDSLFPVAKGGTVAVPGPFGSGKCVAGDTPLLLADS Picrophilus_torridus_DSM_9790 NEGQQVKSNFIIGEVDETEIIKNKIMVPYGV-EGTVKSI-KSGKFKVSDTVAIIETKNGD-YEIKLKQIWPVREARRVFHKFPPEIPLITGQRVIDAFFPVAKGGTVAVPGPFGSGKCVTGDTPVLLADG 261 Location of HE domain Pyrococcus_furiosus_DSM_3638 GLIKIKDLYEKLDGKGRKT--VEGNEEWTELEEPITVYGYKNGKIVEIKATHVYKGASSGMIEIKTRTGRKIKVTPIHKLFTGRVTKDGLVLEEVMAMHIKPGDRIAVVKKIDGGEYILFDEVVEVNYIS Pyrococcus_abyssi_GE5 GLIKIKDLYKILDGKGKKT--VNGNEEWTELERPITLYGYKDGKIVEIKATHVYKGFSAGMIEIRTRTGRKIKVTPIHKLFTGRVTKNGLEIREVMAKDLKKGDRIIVAKKIDGGERVLFDEIVEIRYIS Pyrococcus_horikoshii_OT3 GLIKIKELYEKLDGKGRKI--VEGNEEWTELEKPITVYGYKDGKIVEIKATHVYKGVSSGMVEIRTRTGRKIKVTPIHRLFTGRVTKDGLILKEVMAMHVKPGDRIAVVKKIDGGEYIIFDEVIDVRYIP Pyrococcus_sp._NA2 GLIKIRDLYKILDGKGKKT--INGNEEWTELDNPITLYGYKNGKIVEIKATHIYKGFSAGMIEIKTRTGRKIRVTPIHKLFTGRVTKNGLEIKEVMAMDLKKGDRIIVAKKIDGGERVLFDEIVEIRYIE Thermococcus_litoralis_DSM_5473 GLVKIKELYEKLDGKGRKT--VEGNEEWTELETPVTVYGYRNGRIVGIKATHIYKGISSGMIEIRTRTGRKIKVTPIHKLFTGRVTKDGLALEEVMAMHIKPGDRIAVVKKIDGGEYILFDEVVEVKYIP Thermoplasma_acidophilum_DSM_1728 GERRIGDLFMEAIQDQKNAVEIGQNEEIVRLHDPLRIYSMVGSEIVESVSHAIYHGKSNAIVTVRTENGREVRVTPVHKLF--VKIGN--SVIERPASEVNEGDEIACASVSENGDSLTFDRVVSKEMHS Thermoplasma_volcanium_GSS1 KTIKIKDLYSSERKKEDNIVEAGSGEEIIHLKDPIQIYSYVDGTIVRSRSRLLYKGKSSYLVRIETIGGRSVSVTPVHKLF--VLTEK--GIEEVMASNLKVGDMIAAVAESESEARATFDRVKSIAYEK Thermoplasma_acidophilum GERRIGDLFMEAIRPKERG-EIGQNEEIVRLHDSWRIYSMVGSEIVETVSHAIYHGKSNAIVNVRTENGREVRVTPVHKLF--VKIGN--SVIERPASEVNEGDEIAWPSVSENGDSLTFDRVVSKEMHS Thermoplasmatales_archaeon_I-plasma, SVVKIEELYEKSRVTGKRT--VGIGEEYIETSEPVELFSYQDGEIVESESNLIYKGRSDTVVKIRTATGREVRVTPVHKLF--RVNEKG-DISETMSSHLTKGDRILCTKNRIGNNGVFADEIVETELQY Methanothermus_fervidus_DSM_2088 SLITMEELYKKSLENGKVI-EENEHEEIIKLDKPITVYSFDGSKIIKTKTDIFYKGKSDSLIHIKTKSGREVKVTPIHILF--KINENG-EIVETMAKDLNEGDYIVAARKIDINNEIFADQIVEIREIT Mahella_australiensis_501_BON TLTTMDELFENALCRGKVQ--MQGCETLVELDQPLSVLTMDNGVGIEAQAPIVYKGKSDTLLCVRTHSGRSVKVTPVHKLF--RIDDSG-HIIETQAGDLKPGEFIAAPRRLKADAGFFCDEIVAIDEME Candidatus_Nanosalinarum_sp._J07AB56 SKRAIEDIYHDYEGRGDQT--QRENERWTDLDNGPRVLSRKKGEIVEKQVSTVYKGKTDSTVSVGTRSGREVELTPVHRLF--VLTPEM-EIEEREAQNLQKGDTLLVPRNLPVDSSFHPDRVASIETHQ Thetis_Sea_contig_00086 SKRPI-ELYEEKKGEGERT--EKVREEWTELDRPIGVLSMDDGELKEKEATHVYKGKTRSTIKIKTESGRDIELTPVHKLF--VLTPEM-EILEEKAEDIEEGDTLLLPRKLPIKGRFLPDPVKSVDVEE Ferroplasma_sp._Type_II SIKTIEEVYKEAESEGEIE-YHDENETLIKLKSPLKIYSFYDGKVSESKSDYIYKGKSDSLLKIKTRTGKQVKVTPIHKLF--RLDAEG-NIVETEAEYLKAGDYIVSIRKFEAEEEIYFDRIDSIEYMP Picrophilus_torridus_DSM_9790 TVMSIEDIYNKS--SGTVE-YKNENETLIRLDEPLRLYSFYNGHVNESTSNYIYKGKSDSIIKIRTASGREVKVTPVHKLF--RFVDD--KIIETEARYLNTGDFIASIKRFNNKDELYPDEIESIEILP 391 Intein Extein boundary Pyrococcus_furiosus_DSM_3638 EPQEVYDITTE--THNFVGGNMPTLLHNTVTQHQLAKWSDAQVVVYIGCGERGNEMTDVLEEFPKLKDPNTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVALMADSTSRWAEALRE Pyrococcus_abyssi_GE5 EGQEVYDVTTE--THNFIGGNMPTLLHNTVTQHQLAKWSDAQVVIYIGCGERGNEMTDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVALMADSTSRWAEALRE Pyrococcus_horikoshii_OT3 EPQEVYDVTTE--THNFVGGNMPTLLHNTVTQHQLAKWSDAQVVIYIGCGERGNEMTDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVALMADSTSRWAEALRE Pyrococcus_sp._NA2 MGQEVYDVTTE--THNFVGGNMPTLLHNTVTQHQLAKWSDAQVVVYIGCGERGNEMTDVLEEFPKLKDPKTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVALMADSTSRWAEALRE Thermococcus_litoralis_DSM_5473 EPQEVYDITTE--THNFVGGNMPTLLHNTVTQHQLAKWSDAEVVVYIGCGERGNEMTDVLEEFPKLKDPRTGKPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYNVALMADSTSRWAEALRE Thermoplasma_acidophilum_DSM_1728 GVFDVYDLMVPDYGYNFIGGNGLIVLHNTVIQHQLAKWSDANIVVYIGCGERGNEMTEILTTFPELKDPNTGQPLMDRTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTSRWAEALRE Thermoplasma_volcanium_GSS1 GDFDVYDLSVPEYGRNFIGGEGLLVLHNTVIQHQLAKWSDANIVVYIGCGERGNEMTEILTTFPELKDPVSGQPLMDRTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTSRWAEALRE Thermoplasma_acidophilum GVFDVYDLMVPDYGYNFIGGNGLIVLHNTVIQHQLAKWSDANIVVYIGCGERGNEMTEILTTFPELKDPNTGQPLMTGLSFIANTSNMPVAAREASIYTGITIAEYYRDMGYDVALMADSTSRWAEALRE Thermoplasmatales_archaeon_I-plasma, GPFDVFDLSVPDYGRNFIGGYGGIVLHNTVVQHQLAKWADSEVVIYVGCGERGNEMTEILTTFPELTDPKTGKPLMERTILIANTSNMPVAAREASIYTGVTMAEYFRDMGYNVALMADSTSRWAEALRE Methanothermus_fervidus_DSM_2088 GPFDVYDISIPG-IENFFGGFGAILLHNTVTQQQLAKWADADIVIYVGCGERGNEMTEVLEEFPKLEDPRTGRPLMERTVLIANTSNMPVAAREACVYTGITIAEYFRDMGYDVALMADSTSRWAEAMRE Mahella_australiensis_501_BON GPFDVYDVTVPE-THNFIGGTGALVLHNTVVQHQLAKWADADIIVYVGCGERGNEMTDVLMEFPELKDPRSGQPLMKRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRWAEALRE Candidatus_Nanosalinarum_sp._J07AB56 ETKEVYDLTVPD-THNFVGGNAPMLLHNTVTQQSLAKFSDADVIVYIGCGERGNEMTEVLEEFPELEDPQTGEKLIDRTVLIANTSNMPVAAREASIYTGVTIAEYFRDQGLDVALMADSTSRWAEAMRE Thetis_Sea_contig_00086 EEKEVYDLTVPE-THNFVGGNAPMILHNTVTLHQLAKWSDTQIVVYVGCGERGNEMTDVLLHFPELEDPRTGEPLMDRTALVANTSNMPVAAREASIYTGITIAEYFRDMGYDIALMADSTSRWAEALRE Ferroplasma_sp._Type_II GPFDVYDVTTPDFGSNFVGGEGAILLHNTVIQHQLSKWSDADITVYVGCGERGNEMTEILSTFPELQDPKSGKPLMEKTILIANTSNMPVAAREASIYTGVTIAEYYRDMGYDVALMADSTSRWAEALRE Picrophilus_torridus_DSM_9790 GPFDVYDVTTPDFGSNFVGGYGAILLHNTVIQHQLSKWSDSDIVVYVGCGERGNEMTEILSTFPELMDPKTGKPIMQRTVLIANTSNMPVAAREASIYTGVTIAEYYRDMGYNVALMADSTSRWAEALRE 521 7 Pyrococcus_furiosus_DSM_3638 ISGRLEEMPGEEGYPAYLASRLAEFYERAGRVVTLGSDYRVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVFWALDADLARRRHFPAINWLTSYSLYVDAVQDWWHKNVDPEWRRMRDKAMELLQKEAEL Pyrococcus_abyssi_GE5 ISGRLEEMPGEEGYPAYLASKLAEFYERAGRVVTLGSDYRVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVFWALDADLARRRHFPAINWLTSYSLYVDAVKDWWHKNVDPEWKAMRDKAMELLQKESEL Pyrococcus_horikoshii_OT3 ISGRLEEMPGEEGYPAYLASKLAEFYERAGRVVTLGSDYRVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVFWALDADLARRRHFPAINWLTSYSLYVDAVKDWWHKNIDPEWKAMRDKAMALLQKESEL Pyrococcus_sp._NA2 ISGRLEEMPGEEGYPAYLASKLAEFYERAGRVITLGSDNRVGSVSVIGAVSPPGGDFSEPVVQNTLRVVKVFWALDADLARRRHFPAINWLTSYSLYTDAVKDWWHKNVDPEWKKMRDEAMALLQKEAEL Thermococcus_litoralis_DSM_5473 ISGRLEEMPGEEGYPAYLASKIAEFYERAGRVRTLGSDGRIGSVSVIGAVSPPGGDLSDPVVQNTLRVVKVFWALDADLARRRHFPAINWLTSYSLYVDSIKDWWHKNVDPEWKAMRDEAMALLQKESEL Thermoplasma_acidophilum_DSM_1728 ISGRLEEMPGEEGYPAYLGRRVSEFYERSGRARLVSPDERYGSITVIGAVSPPGGDISEPVSQNTLRVTRVFWALDAALANRRHFPSINWLNSYSLYTEDLRSWYDKNVSSEWSALRERAMEILQRESEL Thermoplasma_volcanium_GSS1 ISGRLEEMPGEEGYPAYLGRRISEFYERSGRARLVSPEDRFGSITVIGAVSPPGGDISEPVSQNTLRVTRVFWALDASLANRRHFPSINWLNSYSLYTEDLRHWYDENVAKDWGSLRSQAMDILQRESEL Thermoplasma_acidophilum ISGRLEEMPGEEGYPAYLGRRVSEFYERSGRARLVSPDERYGSITVIGAVSPPGGDISEPVSQNTLRVTRVFWALDAALANRRHFPSINWLNSYSLYTEDLRSWYDKNVSSEWSALRERAMEILQRESEL Thermoplasmatales_archaeon_I-plasma, ISGRLEEMPGEEGYPAYLGRRVAEFYERAGNSVVLGQKQRTGSVTIIGAVSPPGGDISEPVSQNTLRVTRVFWGLDASLAQRRHFPSVNWLTSYSLYSEDVRKWFEKNVSQEWHSQYLRSMEILQEESEL Methanothermus_fervidus_DSM_2088 ISGRLEEMPGEEGYPAYLASRLAQFYERAGRVITLGSEDKVASVSVIGAVSPPGGDFSEPVTQNTLRICKVFWALDSSLADRRHFPAIDWLQSYSLYVDSVKDWWKKKIGKDWKAVRDEAMALLQKEAEL Mahella_australiensis_501_BON MSGRLEEMPGEEGYPAYLSSRLAEFYERTGYVKCMGSQEREGTLTAVGAVSPPGGDISEPVTQATLRIVKVFWSLDAQLARARHFPAINWLLSYSLYIDNIRDWMNENVAPDWMDLRMQAMRLLQEESSL Candidatus_Nanosalinarum_sp._J07AB56 VSARLEEMPGERGYPAYLASRLAEFYERGGRVRPLGPEE-PGSVSIIGAVSPPGGDFSEPVTQNTLRVVKNFFALDKDLAEKRHFPSINWNDSYSGYSETLSDYWQEEVDEDWNQNVQRLRDLLQESDDL Thetis_Sea_contig_00086 ISGRLEEMPSEEGYPAYLGSRLAEFYERAGRVITSGGGDRKGSITVIGAVSPPGGDFSEPVTQNTLRIIKTFWALDSDLADKRHFPAINWLDSYSGYIDRIEDWWEEEVGANWREYRDKAVSILQEEDEL Ferroplasma_sp._Type_II ISGRLEEMPGEEGYPAYLGRRISEFYERSGNAQIIPQDERTGSITLIGAVSPPGGDLSDPVVQNTLRVTRVFWALDASLASRRHFPSINWLNSYSLYLDSLSGWFKSNVNTEWLQAHSLIMGILQKEAEL Picrophilus_torridus_DSM_9790 ISGRLEEMPGEEGYPAYLGRRISEFYERSGNAQIIAEDQRTGSVTLIGAVSPPGGDLSDPVVQNTLRVTRVFWALDASLASRRHFPSINWLTSYSLYTNNLSKWYTENVGPDWPEIYKTMMDLLEKESEL

651 Pyrococcus_furiosus_DSM_3638 QEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVDTYCPPQKQVTMMRVLMTFYERTMDAISRGVPLEEIAKLPVREEIGRMKF--EPDIEKI-RALIDKTNEQFDELLKKYG--A-- Pyrococcus_abyssi_GE5 QEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVDTYCPPEKQVTMMRVLLNFYDKTMEAISRGVPLEEIAKLPVREEIGRMKF--EPDVGKI-KALIDKTNEQFEELFKKYG--A-- Pyrococcus_horikoshii_OT3 QEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVDTYCPPEKQVTMMRVLLNFYDKTMEAINRGVPLEEIAKLPVREEIGRMKF--ERDVSKI-RSLIDKTNEQFEELFKKYG--A-- Pyrococcus_sp._NA2 QEIVRIVGPDALPERERAILLVARMLREDYLQQDAFDEVDTYCPPEKQVTMMRVLLNFYHKTMEAINRGIPLEEIAKLPVREEIGRMKF--ERDIEKI-RELIDKTNEQFEELFKKYG--A-- Thermococcus_litoralis_DSM_5473 EEIVRIVGPDALPERERAILLVARMIREDYLQQDAFHEVDTYCPPKKQITMMKVILNFYHYTMRAIDMGVPVEEIAKLPVREEIGRMKY--NPNVEEI-ASLMKKTEEQFEELFKKYG--E-- Thermoplasma_acidophilum_DSM_1728 QEVAQLVGYDAMPEKEKSILDVARIIREDFLQQSAFDEIDAYCSLKKQYLMLKAIMEIDTYQNKALDSGATMDNLASLAVREKLSRMKIVPEAQVESYYNDLVEEIHKEYGNFIGEKNAEASL Thermoplasma_volcanium_GSS1 QEVAQLVGYDAMPEKEKSILDVARIIREDFLQQSAFDEIDSYCSLRKQYLMLKAIMELNSYQSMAIDHGVTMDNLSSLPVREKLSRMKIVPEDQVESYYSSIIKEIHKEYTSFIGEKNAEANI Thermoplasma_acidophilum QEVAQLVGYDAMPEKEKSILDVARIIREDFLQQSAFDEIDAYCSLKKQYLMLKAIMEIDTYQNKALDSGATMDNLASLAVREKLSRMKIVPEAQVESYYNDLVEEIHKEYGNFIGEKNAEASL Thermoplasmatales_archaeon_I-plasma, QEVAQLVGYDALPENEKEVLDVARAIREYFLQQSAFDDVDTYCSLKKQFLILKSILQMDQLEKYAVSKGAAVSDLNSLSYREKLSRFKEVKEGDVEQYFTSLSKLMEDE------Methanothermus_fervidus_DSM_2088 QEIVQLVGPDALPDIERLTLETARMIREDFLQQNAYHEIDTYCPPEKQFKMLETILLFNRETKKALKKGAPIDKLTQLPVKEDIARMKYIPPDEFEEKVKEIQDKIIKQCEEVLK------Mahella_australiensis_501_BON EEIVRLVGIDAISIRDRWVLEAARSIREDFLHQVAFDEVDTYTSMNKQYRMLKIIMDYYKRGQQALDEGVELDAITNLPVRERIDRAKYIKEENMNQF-DDIEKQMAEQFAQLLESSK--A-- Candidatus_Nanosalinarum_sp._J07AB56 EETVQLVGKDALPDRDRLTLEIGDMLKEFYLQQNAFHPVDQYSSPQKTFDMLEVILEYADHAYEALDEGALVEDIVSLNSRAEIGRIKT--AEDHREKLEEVREQMQDEFGEVAEQ------Thetis_Sea_contig_00086 REIVQLVGPDALPDRDQAVLEAARVIREDFLQQNAMHEVDTYCSVEKQLRMFEIVLDFYDETVGAVEEGGFGRGGHSAPVRRNIARMKTIPDEKFMEQADEIEDEIDDQIEEVMEEGD----- Ferroplasma_sp._Type_II QEIVQLVGYDSLPEKQKNILDIAKIIREDFLQQNAFDDTDTYCSIKKQYEMLMIIKTLNEMQEKAIDAGLKVIQTATLPVRMKISRMKEITENDFERFYNEVIKEINNEYDNIMEGVE---NV Picrophilus_torridus_DSM_9790 QEIVQLVGYDALPEKEKNVLDIAKMIREDFLQQNAFDDIDTYCSIKKQYMMLKIIKTVYEMQMNALNHGMKISQITSIPARSKISRMKEVSEQDFPAFYKNIIKEINDEYNSMIEVGG--VNA Alignment of Intein Sequences

** *:* : : : ::: * : . .*:* : : : * *:.: :**:* ** . : * : : *: : Pyrococcus_furiosus_DSM_3638 CVDGDTLILTKEFGLIKIKDLYEKLDGKGRKT--VEGNEEWTELEEPITVYGYKNGKIVEIKATHVYKGASSGMIEIKTRTGRKIKVTPIHKLFTGRVTKDGLVLEEVMAMHIKPGDRIAVVKKIDGGEY------128 Pyrococcus_abyssi_GE5 CVDGDTLVLTKEFGLIKIKDLYKILDGKGKKT--VNGNEEWTELERPITLYGYKDGKIVEIKATHVYKGFSAGMIEIRTRTGRKIKVTPIHKLFTGRVTKNGLEIREVMAKDLKKGDRIIVAKKIDGGER------128 Pyrococcus_horikoshii_OT3 CVDGDTLVLTKEFGLIKIKELYEKLDGKGRKI--VEGNEEWTELEKPITVYGYKDGKIVEIKATHVYKGVSSGMVEIRTRTGRKIKVTPIHRLFTGRVTKDGLILKEVMAMHVKPGDRIAVVKKIDGGEY------128 Pyrococcus_sp._NA2 CVDGDTLVLTKEFGLIKIRDLYKILDGKGKKT--INGNEEWTELDNPITLYGYKNGKIVEIKATHIYKGFSAGMIEIKTRTGRKIRVTPIHKLFTGRVTKNGLEIKEVMAMDLKKGDRIIVAKKIDGGER------128 Thermococcus_litoralis_DSM_5473 CVDGNTLVLTEEFGLVKIKELYEKLDGKGRKT--VEGNEEWTELETPVTVYGYRNGRIVGIKATHIYKGISSGMIEIRTRTGRKIKVTPIHKLFTGRVTKDGLALEEVMAMHIKPGDRIAVVKKIDGGEY------128 Thermoplasma_acidophilum_DSM_1728 CVSGDTPVLL-DAGERRIGDLFMEAIQDQKNAVEIGQNEEIVRLHDPLRIYSMVGSEIVESVSHAIYHGKSNAIVTVRTENGREVRVTPVHKLF--VKIGN--SVIERPASEVNEGDEIACASVSENGDS------125 Thermoplasma_volcanium_GSS1 CVSGETPVYLADGKTIKIKDLYSSERKKEDNIVEAGSGEEIIHLKDPIQIYSYVDGTIVRSRSRLLYKGKSSYLVRIETIGGRSVSVTPVHKLF--VLTEK--GIEEVMASNLKVGDMIAAVAESESEARDCGM------130 Thermoplasma_acidophilum CVSGDTPVLL-DAGERRIGDLFMEAIRPKERG-EIGQNEEIVRLHDSWRIYSMVGSEIVETVSHAIYHGKSNAIVNVRTENGREVRVTPVHKLF--VKIGN--SVIERPASEVNEGDEIAWPSVSENGDS------124 Thermoplasmatales_archaeon_I-plasma CVTGDTPVLLRDGSVVKIEELYEKSRVTGKRT--VGIGEEYIETSEPVELFSYQDGEIVESESNLIYKGRSDTVVKIRTATGREVRVTPVHKLF--RVNEKG-DISETMSSHLTKGDRILCTKNRIGNNG------125 Methanothermus_fervidus_DSM_2088 CVSGDTPILLGDGSLITMEELYKKSLENGKVI-EENEHEEIIKLDKPITVYSFDGSKIIKTKTDIFYKGKSDSLIHIKTKSGREVKVTPIHILF--KINENG-EIVETMAKDLNEGDYIVAARKIDINNEDEKIDLSKF------135 Mahella_australiensis_50-1_BON CVSGDTPIVLADGTLTTMDELFENALCRGKVQ--MQGCETLVELDQPLSVLTMDNGVGIEAQAPIVYKGKSDTLLCVRTHSGRSVKVTPVHKLF--RIDDSG-HIIETQAGDLKPGEFIAAPRRLKADAGDAAFEL-EISPNVRVMDDD- 143 Candidatus_Nanosalinarum_sp._J07AB56 CVVGDTPVALADGSKRAIEDIYHDYEGRGDQT--QRENERWTDLDNGPRVLSRKKGEIVEKQVSTVYKGKTDSTVSVGTRSGREVELTPVHRLF--VLTPEM-EIEEREAQNLQKGDTLLVPRNLPVDSSNVEIEVAEALPEKRVVGNGF 145 Thetis_Sea_contig_00086_gi|3548555569/238-759 CVTGETPVLLSDGSKRPI-ELYEEKKGEGERT--EKVREEWTELDRPIGVLSMDDGELKEKEATHVYKGKTRSTIKIKTESGRDIELTPVHKLF--VLTPEM-EILEEKAEDIEEGDTLLLPRKLPIKGRREEIEAERLVPEKRVCGEDF 144 Ferroplasma_sp._Type_II CVAGDTPLLLADSSIKTIEEVYKEAESEGEIE-YHDENETLIKLKSPLKIYSFYDGKVSESKSDYIYKGKSDSLLKIKTRTGKQVKVTPIHKLF--RLDAEG-NIVETEAEYLKAGDYIVSIRKFEAEEEYKHIDAYSLFGDARATSNEI 146 Picrophilus_torridus_DSM_9790 CVTGDTPVLLADGTVMSIEDIYNKS--SGTVE-YKNENETLIRLDEPLRLYSFYNGHVNESTSNYIYKGKSDSIIKIRTASGREVKVTPVHKLF--RFVDD--KIIETEARYLNTGDFIASIKRFNNKDE------123 1...... 10...... 20...... 30...... 40...... 50...... 60...... 70...... 80...... 90...... 100...... 110...... 120...... 130...... 140...... 150

Pyrococcus_furiosus_DSM_3638 ------VKL----DTSSVTKIKVPEVLNEELAEFLGYVIGDGTLKPRT--VAIYNNDESLL-KRANFLAMKLFGVSGKI--VQERTVKALLIHSKYLVD 212 Pyrococcus_abyssi_GE5 ------VKLNIRVEQKRGKKIRIPDVLDEKLAEFLGYLIADGTLKPRT--VAIYNNDESLL-RRANELANELFNIEGKI--VKGRTVKALLIHSKALVE 216 Pyrococcus_horikoshii_OT3 ------IKL----DSSNVGEIKVPEILNEELAEFLGYLMANGTLKSGI--IEIYCDDESLL-ERVNSLSLKLFGVGGRI--VQKVDGKALVIQSKPLVD 212 Pyrococcus_sp._NA2 ------VKLNIKVDQKRGKKIKIPEVLDEKLAEFLGYLIADGTLKPRT--VAIYNNDESIL-KRANELAKDLFNVEGKI--IKEKTVKALLIHSKALVE 216 Thermococcus_litoralis_DSM_5473 ------VKLTTSPDFRKSRKIKVPEVLDEDLAEFLGYLIADGTLKPRT--VAIYNNDESLL-KRANFLSTKLFGINGKI--VQERTVKALLIHSKPLVD 216 Thermoplasma_acidophilum_DSM_1728 ------125 Thermoplasma_volcanium_GSS1 ------130 Thermoplasma_acidophilum ------124 Thermoplasmatales_archaeon_I-plasma ------AGSSGMMMAQ------135 Methanothermus_fervidus_DSM_2088 ------NEKEIKKLPDKMSPELAEFLGLLFAAGQLDENKLILKIENGNKDIL-ERFSELSLKLFGVECKI--LENKAI----IENKLIVK 212 Mahella_australiensis_50-1_BON --VRRNISVILRNMRANGTLDSVVGLSRASAESIIYGKLTASVETVGNIYAAAELELPRFNNLRGARAGHIVRLPQKVTSELAELAGLFIAEGHIRDEG--TVIFTNSEARLRQRFKDLIMKVFGIPCKDV-LQSGKTPSVAVFSTTLVC 288 Candidatus_Nanosalinarum_sp._J07AB56 EAVREAITVLEENHGTRKAAAEHLGIDDHRMDAYATGRNRPRVADATAILEEAG-KTHKLRCVKGEKQSKPTRIPEEVDEDFAELLGLLLGDGTVKPRS--VQFYNNNERLL-DRVEHLAEKLFGLKSAR--TTANTVESVRVDSKVLRD 289 Thetis_Sea_contig_00086_gi|3548555569/238-759 EKVRGSIKKLEKEFETRKELAEELGVTEEVLTEYSLGRNRPRVKLASKIIEMSG-EEHSIRNIKGENHAKRIKLPEKMDEELAELLGFLLGDGSLKPKS--VCFYNNDEELL-SRVEELLEEIFEVQAKR--EYASTVKSVRVGSKALRD 288 Ferroplasma_sp._Type_II KNKLKEVTK-----ENRKLLKNIINERILDQITRNEYNPAPKLNWIKTIYDELNLQRPEIKCLRGDRNGNIVTVPDYVSEDLSELLGFYVAEGYIRGKS--TFVITDSDEKIIGRVKYLLKKVFNLNGKI-EIQKNKTNNLIVNSIILSE 288 Picrophilus_torridus_DSM_9790 ------NYLSGDESELLGLYASYGSIEDGILI------DASIKDRFINLAMNIFKLKTIKIEYRN---DRVLIKNDGLKD 188 ...... 160...... 170...... 180...... 190...... 200...... 210...... 220...... 230...... 240...... 250...... 260...... 270...... 280...... 290...... 300

Pyrococcus_furiosus_DSM_3638 FLKKLGIPGNKKARTWKVPKELLLSPPSVVKAFINAYIACDGYYNKEKGEIEIVTASEEGAYGLTYLLAKLGIYATIRRKT-INGREYYRVVISGKANLEKL------GVKREARGYTSIDVVPVDVESIYEAL 339 Pyrococcus_abyssi_GE5 FFSKLGVPRNKKARTWKVPKELLISEPEVVKAFIKAYIMCDGYYDENKGEIEIVTASEEAAYGFSYLLAKLGIYAIIREKI-IGDKVYYRVVISGESNLEKL------GIERVGRGYTSYDIVPVEVEELYNAL 343 Pyrococcus_horikoshii_OT3 VLRRLGVPEDKKVENWKVPRELLLSPSNVVRAFVNAYI------KGKEEVEITLASEEGAYELSYLFAKLGIYVTISKSG-----EYYKVRVSRRGNL------DTIPVEVNGMPKVL 313 Pyrococcus_sp._NA2 FFSMLGVPRSKKARTWKVPKELLISEPEVVKAFIKAYIMCDGHYHEKKGEIEIVTASEEAAYGLSYLLAKLGIYAIIREKV-IKDRTYYRVIISGKSNLEKL------GIARMGRGYTSFDVVPMDVEELYDAI 343 Thermococcus_litoralis_DSM_5473 FFRKLGIPESKKARNWKVPRELLLSPPSVVKAFINAYIVCDGYYHERKGEIEITTASEEGAYGLSYLLAKLGIYATFRKKQ-IKGKEYYRIAISGKTNLEKL------GIKRETRGYTNIDIVPVEVESIYNAL 343 Thermoplasma_acidophilum_DSM_1728 ------125 Thermoplasma_volcanium_GSS1 ------130 Thermoplasma_acidophilum ------124 Thermoplasmatales_archaeon_I-plasma ------135 Methanothermus_fervidus_DSM_2088 FVKTLGILES------KIPKIILKSKNKCIARFIDGYINASASNN------VLSIEDKDLKVQLSYLLTRAGIYHEISHDG------IFLHLDNSL------LTSVEYGDEVVSVAISSC 308 Mahella_australiensis_50-1_BON IFEAMGMAGNSKHK--SVPPVIMRSSDKALAAFLRGYYLGDGSFS--EGEIEFSTASPRLQIAVSYALTRLGIFHTMANSG-----RRHRIFVRGRDNLRAFYEFTAGGFADHLKFNAIMEYVNSKKTTYTAHDIVPVGPELIERVYEAA 429 Candidatus_Nanosalinarum_sp._J07AB56 LLVYLGFPETEKSLNCSVPETVMKGSEDVVAAFVRGYFLADGHFS--EYEAELSTSSRRMQEDLAYALTRIGITPRVSEKQ-TDKNPHFRVRFSGDQ----LIEFHRKLEADYDKFEQIEEYLDRVEDHFRGTESVDIAPETVRSAFESS 432 Thetis_Sea_contig_00086_gi|3548555569/238-759 FLAALGFPCEKKSKNCFVPEKVMKGDEDVSASFLRGYYLADGSFN--KYEVEISTSSKEMSEDLAYLLTKIGVSSRIGSRE-TKASRSYRVRISGEE----LEEFYDKTSSDHIKYEEIEQYLEESSGHFKGVDSVEVSPDLVMDSFENA 431 Ferroplasma_sp._Type_II FIRKLI--PGKLASHKDIPQIIMKSKDKVIWAFLKGYYLGDGSYY--NGNIELSTASKKLSISLSYLLTRLGILHTIKNDKSLNKNYRYRTFIRGLNELKKFYLNIAPGNENFQKIDNIKKYTDSKNSTYTAIDLVPLGAQKIDDLYR-T 433 Picrophilus_torridus_DSM_9790 FIARMI------SSGIPSEVMRSR-ACAASFINGYLYGKLYH---DDVIKLHDNEQNI-LKISYMLTGLGIIHSIRNNL------256 ...... 310...... 320...... 330...... 340...... 350...... 360...... 370...... 380...... 390...... 400...... 410...... 420...... 430...... 440...... 450

* : :*:*: **.** :*** Pyrococcus_furiosus_DSM_3638 GRPYSELKKEGIEIHNYL-SGENMSYETFRKFAKVVG-----LEEIA------ENHLQHILFDEVVEVNYISEPQEVYDITTE--THNFVGGNMPTLLHN 425 Pyrococcus_abyssi_GE5 GRPYAELKRAGIEIHNYL-SGENMSYEMFRKFAKFVG-----MEEIA------ENHLTHVLFDEIVEIRYISEGQEVYDVTTE--THNFIGGNMPTLLHN 429 Pyrococcus_horikoshii_OT3 ------PYEDFRKFAKSIG-----LEEVA------ENHLQHIIFDEVIDVRYIPEPQEVYDVTTE--THNFVGGNMPTLLHN 376 Pyrococcus_sp._NA2 GRSYAELKKAGIEIHNYL-SGENMSYEMFKRFAKFVG-----MEEIA------ENHLSHVLFDEIVEIRYIEMGQEVYDVTTE--THNFVGGNMPTLLHN 429 Thermococcus_litoralis_DSM_5473 GRPYSELKGEGIEIHNYL-NGENMTYETFRKFAKLVG-----LEEVA------ENHLKHILFDEVVEVKYIPEPQEVYDITTE--THNFVGGNMPTLLHN 429 Thermoplasma_acidophilum_DSM_1728 ------QTVTTTLV------LTFDRVVSKEMHSGVFDVYDLMVPDYGYNFIGGNGLIVLHN 174 Thermoplasma_volcanium_GSS1 ------SEECVMEAEVYTSLE------ATFDRVKSIAYEKGDFDVYDLSVPEYGRNFIGGEGLLVLHN 186 Thermoplasma_acidophilum ------QTVTTTLV------LTFDRVVSKEMHSGVFDVYDLMVPDYGYNFIGGNGLIVLHN 173 Thermoplasmatales_archaeon_I-plasma ------QINEVFADEIVETELQYGPFDVFDLSVPDYGRNFIGGYGGIVLHN 180 Methanothermus_fervidus_DSM_2088 GE------TNYPNEIFADQIVEIREITGPFDVYDISIPG-IENFFGGFGAILLHN 356 Mahella_australiensis_50-1_BON GRPYARLKKAGIEITNYTRNGERMSADKFKKFVEITGDPTGELEQLC------LYLDDFFCDEIVAIDEMEGPFDVYDVTVPE-THNFIGGTGALVLHN 521 Candidatus_Nanosalinarum_sp._J07AB56 EATRADLKSAGAKLSNYETQEERISVPALQNFADVTK--NKHLAEMA------GNHLEHFHPDRVASIETHQETKEVYDLTVPD-THNFVGGNAPMLLHN 523 Thetis_Sea_contig_00086_gi|3548555569/238-759 SLTRESFKERGIKIANYTTQEERMSVPTLQKFSSLAG--NEKMQKIA------QNHLTHFLPDPVKSVDVEEEEKEVYDLTVPE-THNFVGGNAPMILHN 522 Ferroplasma_sp._Type_II HPSYNKLKSNEVEITNYTGQNEIMSVSSFNKFARTILLNNKEEEDNQDINTLSHLSEYLDFIYFDRIDSIEYMPGPFDVYDVTTPDFGSNFVGGEGAILLHN 535 Picrophilus_torridus_DSM_9790 ----IEIKAENMKILNSMENE------LIDNNETLLISNNANDDFD------LYPDEIESIEILPGPFDVYDVTTPDFGSNFVGGYGAILLHN 333 ...... 460...... 470...... 480...... 490...... 500...... 510...... 520...... 530...... 540...... 550..

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