DOCSLIB.ORG

Mouse Wipf1 Knockout Project (CRISPR/Cas9)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Mouse Wipf1 Knockout Project (CRISPR/Cas9) https://www.alphaknockout.com Mouse Wipf1 Knockout Project (CRISPR/Cas9) Objective: To create a Wipf1 knockout Mouse model (C57BL/6J) by CRISPR/Cas-mediated genome engineering. Strategy summary: The Wipf1 gene (NCBI Reference Sequence: NM_153138 ; Ensembl: ENSMUSG00000075284 ) is located on Mouse chromosome 2. 8 exons are identified, with the ATG start codon in exon 2 and the TGA stop codon in exon 8 (Transcript: ENSMUST00000094681). Exon 3~6 will be selected as target site. Cas9 and gRNA will be co-injected into fertilized eggs for KO Mouse production. The pups will be genotyped by PCR followed by sequencing analysis. Note: Homozygous mutants have immunological abnormalities, although lymphocyte development appears normal. Mutants show abnormal B and T cell proliferative responses, high serum immunoglobulin levels and impaired immunological synapse formation. Exon 3 starts from about 3.52% of the coding region. Exon 3~6 covers 85.26% of the coding region. The size of effective KO region: ~9629 bp. The KO region does not have any other known gene. Page 1 of 9 https://www.alphaknockout.com Overview of the Targeting Strategy Wildtype allele 5' gRNA region gRNA region 3' 1 3 4 5 6 8 Legends Exon of mouse Wipf1 Knockout region Page 2 of 9 https://www.alphaknockout.com Overview of the Dot Plot (up) Window size: 15 bp Forward Reverse Complement Sequence 12 Note: The 2000 bp section upstream of Exon 3 is aligned with itself to determine if there are tandem repeats. No significant tandem repeat is found in the dot plot matrix. So this region is suitable for PCR screening or sequencing analysis. Overview of the Dot Plot (down) Window size: 15 bp Forward Reverse Complement Sequence 12 Note: The 802 bp section downstream of Exon 6 is aligned with itself to determine if there are tandem repeats. No significant tandem repeat is found in the dot plot matrix. So this region is suitable for PCR screening or sequencing analysis. Page 3 of 9 https://www.alphaknockout.com Overview of the GC Content Distribution (up) Window size: 300 bp Sequence 12 Summary: Full Length(2000bp) | A(29.2% 584) | C(20.75% 415) | T(26.75% 535) | G(23.3% 466) Note: The 2000 bp section upstream of Exon 3 is analyzed to determine the GC content. No significant high GC-content region is found. So this region is suitable for PCR screening or sequencing analysis. Overview of the GC Content Distribution (down) Window size: 300 bp Sequence 12 Summary: Full Length(802bp) | A(26.43% 212) | C(24.69% 198) | T(30.42% 244) | G(18.45% 148) Note: The 802 bp section downstream of Exon 6 is analyzed to determine the GC content. No significant high GC-content region is found. So this region is suitable for PCR screening or sequencing analysis. Page 4 of 9 https://www.alphaknockout.com BLAT Search Results (up) QUERY SCORE START END QSIZE IDENTITY CHROM STRAND START END SPAN ----------------------------------------------------------------------------------------------- browser details YourSeq 2000 1 2000 2000 100.0% chr2 - 73444575 73446574 2000 browser details YourSeq 25 1025 1057 2000 87.9% chr6 + 133223149 133223181 33 browser details YourSeq 25 1792 1830 2000 82.1% chr1 + 74824080 74824118 39 browser details YourSeq 23 850 873 2000 100.0% chr9 + 58506331 58506356 26 browser details YourSeq 22 857 878 2000 100.0% chr9 - 98157020 98157041 22 browser details YourSeq 22 262 283 2000 100.0% chr5 - 118320931 118320952 22 Note: The 2000 bp section upstream of Exon 3 is BLAT searched against the genome. No significant similarity is found. BLAT Search Results (down) QUERY SCORE START END QSIZE IDENTITY CHROM STRAND START END SPAN ----------------------------------------------------------------------------------------------- browser details YourSeq 802 1 802 802 100.0% chr2 - 73434144 73434945 802 browser details YourSeq 108 300 422 802 95.2% chr13 - 52512843 52513020 178 browser details YourSeq 106 304 429 802 92.3% chr2 - 28697289 28697411 123 browser details YourSeq 106 300 427 802 90.6% chr14 + 99356327 99356450 124 browser details YourSeq 103 300 774 802 79.4% chr15 - 93727405 93727575 171 browser details YourSeq 102 300 427 802 87.2% chr19 - 46030559 46030676 118 browser details YourSeq 102 304 424 802 94.0% chr1 + 39804011 39804131 121 browser details YourSeq 101 305 427 802 92.0% chrX - 51751905 51752025 121 browser details YourSeq 101 54 419 802 89.9% chr12 - 10474974 10475527 554 browser details YourSeq 101 305 450 802 93.9% chr11 + 117785997 117786469 473 browser details YourSeq 100 300 420 802 92.2% chr11 - 50167736 50167855 120 browser details YourSeq 98 300 415 802 95.5% chr9 - 72754448 72754577 130 browser details YourSeq 98 300 426 802 85.4% chr14 + 122194552 122194667 116 browser details YourSeq 97 304 426 802 91.5% chr7 - 90877760 90877896 137 browser details YourSeq 97 305 414 802 92.6% chr19 - 56899878 56899985 108 browser details YourSeq 97 295 414 802 91.6% chr15 + 64973827 64973953 127 browser details YourSeq 96 304 427 802 87.8% chr2 - 123254262 123254381 120 browser details YourSeq 96 305 422 802 91.0% chr14 - 83596060 83596175 116 browser details YourSeq 96 305 415 802 94.6% chr15 + 81160573 81160702 130 browser details YourSeq 95 305 422 802 91.1% chr6 - 135534004 135534120 117 Note: The 802 bp section downstream of Exon 6 is BLAT searched against the genome. No significant similarity is found. Page 5 of 9 https://www.alphaknockout.com Gene and protein information: Wipf1 WAS/WASL interacting protein family, member 1 [ Mus musculus (house mouse) ] Gene ID: 215280, updated on 12-Aug-2019 Gene summary Official Symbol Wipf1 provided by MGI Official Full Name WAS/WASL interacting protein family, member 1 provided by MGI Primary source MGI:MGI:2178801 See related Ensembl:ENSMUSG00000075284 Gene type protein coding RefSeq status VALIDATED Organism Mus musculus Lineage Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus Also known as WIP; Waspip; AI115543; D2Ertd120e Expression Broad expression in spleen adult (RPKM 10.8), thymus adult (RPKM 7.3) and 25 other tissues See more Orthologs human all Genomic context Location: 2 C3; 2 43.68 cM See Wipf1 in Genome Data Viewer Exon count: 13 Annotation release Status Assembly Chr Location 108 current GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (73429610..73529487, complement) Build 37.2 previous assembly MGSCv37 (GCF_000001635.18) 2 NC_000068.6 (73267674..73367467, complement) Chromosome 2 - NC_000068.7 Page 6 of 9 https://www.alphaknockout.com Transcript information: This gene has 7 transcripts Gene: Wipf1 ENSMUSG00000075284 Description WAS/WASL interacting protein family, member 1 [Source:MGI Symbol;Acc:MGI:2178801] Gene Synonyms D2Ertd120e, WIP, Waspip Location Chromosome 2: 73,429,610-73,529,734 reverse strand. GRCm38:CM000995.2 About this gene This gene has 7 transcripts (splice variants), 175 orthologues, 2 paralogues, is a member of 1 Ensembl protein family and is associated with 39 phenotypes. Transcripts Name Transcript ID bp Protein Translation ID Biotype CCDS UniProt Flags Wipf1-201 ENSMUST00000094681.10 4728 493aa ENSMUSP00000092268.4 Protein coding CCDS16131 Q8K1I7 TSL:1 GENCODE basic APPRIS P1 Wipf1-203 ENSMUST00000102680.7 4032 493aa ENSMUSP00000099741.1 Protein coding CCDS16131 Q8K1I7 TSL:1 GENCODE basic APPRIS P1 Wipf1-202 ENSMUST00000102679.7 2039 493aa ENSMUSP00000099740.1 Protein coding CCDS16131 Q8K1I7 TSL:1 GENCODE basic APPRIS P1 Wipf1-205 ENSMUST00000141264.7 717 196aa ENSMUSP00000119190.1 Protein coding - F6QWW7 CDS 3' incomplete TSL:2 Wipf1-207 ENSMUST00000151939.1 532 58aa ENSMUSP00000121335.1 Protein coding - F6RQI2 CDS 3' incomplete TSL:3 Wipf1-206 ENSMUST00000143061.1 374 No protein - lncRNA - - TSL:5 Wipf1-204 ENSMUST00000129594.1 354 No protein - lncRNA - - TSL:5 Page 7 of 9 https://www.alphaknockout.com 120.12 kb Forward strand 73.42Mb 73.44Mb 73.46Mb 73.48Mb 73.50Mb 73.52Mb Genes D230022J07Rik-201 >lncRNA Gm13708-201 >lncRNA (Comprehensive set... Gm13707-201 >lncRNA Contigs AL928813.10 > Genes < Wipf1-201protein coding (Comprehensive set... < Wipf1-203protein coding < Wipf1-202protein coding < Wipf1-205protein coding < Wipf1-207protein coding < Wipf1-206lncRNA < Wipf1-204lncRNA Regulatory Build 73.42Mb 73.44Mb 73.46Mb 73.48Mb 73.50Mb 73.52Mb Reverse strand 120.12 kb Regulation Legend CTCF Enhancer Open Chromatin Promoter Promoter Flank Transcription Factor Binding Site Gene Legend Protein Coding merged Ensembl/Havana Ensembl protein coding Non-Protein Coding RNA gene Page 8 of 9 https://www.alphaknockout.com Transcript: ENSMUST00000094681 < Wipf1-201protein coding Reverse strand 100.12 kb ENSMUSP00000092... MobiDB lite Low complexity (Seg) SMART WH2 domain Pfam WH2 domain PROSITE profiles WH2 domain PANTHER PTHR23202 PTHR23202:SF32 Gene3D PH-like domain superfamily All sequence SNPs/i... Sequence variants (dbSNP and all other sources) Variant Legend inframe deletion missense variant synonymous variant Scale bar 0 60 120 180 240 300 360 420 493 We wish to acknowledge the following valuable scientific information resources: Ensembl, MGI, NCBI, UCSC. Page 9 of 9.
Load more

Recommended publications
  • Regulation of Cdc42 and Its Effectors in Epithelial Morphogenesis Franck Pichaud1,2,*, Rhian F
    © 2019. Published by The Company of Biologists Ltd | Journal of Cell Science (2019) 132, jcs217869. doi:10.1242/jcs.217869 REVIEW SUBJECT COLLECTION: ADHESION Regulation of Cdc42 and its effectors in epithelial morphogenesis Franck Pichaud1,2,*, Rhian F. Walther1 and Francisca Nunes de Almeida1 ABSTRACT An overview of Cdc42 Cdc42 – a member of the small Rho GTPase family – regulates cell Cdc42 was discovered in yeast and belongs to a large family of small – polarity across organisms from yeast to humans. It is an essential (20 30 kDa) GTP-binding proteins (Adams et al., 1990; Johnson regulator of polarized morphogenesis in epithelial cells, through and Pringle, 1990). It is part of the Ras-homologous Rho subfamily coordination of apical membrane morphogenesis, lumen formation and of GTPases, of which there are 20 members in humans, including junction maturation. In parallel, work in yeast and Caenorhabditis elegans the RhoA and Rac GTPases, (Hall, 2012). Rho, Rac and Cdc42 has provided important clues as to how this molecular switch can homologues are found in all eukaryotes, except for plants, which do generate and regulate polarity through localized activation or inhibition, not have a clear homologue for Cdc42. Together, the function of and cytoskeleton regulation. Recent studies have revealed how Rho GTPases influences most, if not all, cellular processes. important and complex these regulations can be during epithelial In the early 1990s, seminal work from Alan Hall and his morphogenesis. This complexity is mirrored by the fact that Cdc42 can collaborators identified Rho, Rac and Cdc42 as main regulators of exert its function through many effector proteins.
    [Show full text]
  • The Guanine Nucleotide Exchange Factor Arhgef5 Plays Crucial Roles in Src-Induced Podosome Formation
    1726 Research Article The guanine nucleotide exchange factor Arhgef5 plays crucial roles in Src-induced podosome formation Miho Kuroiwa, Chitose Oneyama, Shigeyuki Nada and Masato Okada* Department of Oncogene Research, Research institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan *Author for correspondence ([email protected]) Accepted 19 January 2011 Journal of Cell Science 124, 1726-1738 © 2011. Published by The Company of Biologists Ltd doi:10.1242/jcs.080291 Summary Podosomes and invadopodia are actin-rich membrane protrusions that play a crucial role in cell adhesion and migration, and extracellular matrix remodeling in normal and cancer cells. The formation of podosomes and invadopodia is promoted by upregulation of some oncogenic molecules and is closely related to the invasive potential of cancer cells. However, the molecular mechanisms underlying the podosome and invadopodium formation still remain unclear. Here, we show that a guanine nucleotide exchange factor (GEF) for Rho family GTPases (Arhgef5) is crucial for Src-induced podosome formation. Using an inducible system for Src activation, we found that Src-induced podosome formation depends upon the Src SH3 domain, and identified Arhgef5 as a Src SH3-binding protein. RNA interference (RNAi)-mediated depletion of Arhgef5 caused robust inhibition of Src-dependent podosome formation. Overexpression of Arhgef5 promoted actin stress fiber remodeling through activating RhoA, and the activation of RhoA or Cdc42 was required for Src-induced podosome formation. Arhgef5 was tyrosine-phosphorylated by Src and bound to Src to positively regulate its activity. Furthermore, the pleckstrin homology (PH) domain of Arhgef5 was required for podosome formation, and Arhgef5 formed a ternary complex with Src and phosphoinositide 3-kinase when Src and/or Arhgef5 were upregulated.
    [Show full text]
  • Microrna Regulatory Pathways in the Control of the Actin–Myosin Cytoskeleton
    cells Review MicroRNA Regulatory Pathways in the Control of the Actin–Myosin Cytoskeleton , , Karen Uray * y , Evelin Major and Beata Lontay * y Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary; [email protected] * Correspondence: [email protected] (K.U.); [email protected] (B.L.); Tel.: +36-52-412345 (K.U. & B.L.) The authors contributed equally to the manuscript. y Received: 11 June 2020; Accepted: 7 July 2020; Published: 9 July 2020 Abstract: MicroRNAs (miRNAs) are key modulators of post-transcriptional gene regulation in a plethora of processes, including actin–myosin cytoskeleton dynamics. Recent evidence points to the widespread effects of miRNAs on actin–myosin cytoskeleton dynamics, either directly on the expression of actin and myosin genes or indirectly on the diverse signaling cascades modulating cytoskeletal arrangement. Furthermore, studies from various human models indicate that miRNAs contribute to the development of various human disorders. The potentially huge impact of miRNA-based mechanisms on cytoskeletal elements is just starting to be recognized. In this review, we summarize recent knowledge about the importance of microRNA modulation of the actin–myosin cytoskeleton affecting physiological processes, including cardiovascular function, hematopoiesis, podocyte physiology, and osteogenesis. Keywords: miRNA; actin; myosin; actin–myosin complex; Rho kinase; cancer; smooth muscle; hematopoiesis; stress fiber; gene expression; cardiovascular system; striated muscle; muscle cell differentiation; therapy 1. Introduction Actin–myosin interactions are the primary source of force generation in mammalian cells. Actin forms a cytoskeletal network and the myosin motor proteins pull actin filaments to produce contractile force. All eukaryotic cells contain an actin–myosin network inferring contractile properties to these cells.
    [Show full text]
  • WASL Knockout Hela Cell Lysate
    Leader in Biomolecular Solutions for Life Science WASL Knockout HeLa Cell Lysate Catalog No.: RM02299 Basic Information Background Catalog No. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott- RM02299 Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed Category in neural tissues, and interacts with several proteins involved in cytoskeletal organization, Cell Lysate including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013] Description WASL Knockout HeLa Cell Line is engineered from HeLa cell line with CRISPR/Cas9 technology. Allele-1:1bp insertion in exon1 Allele-2:4bp deletion in exon1 Gene Information Product Information Gene Symbol Parental Cell line Amount Genotype WASL HeLa 50μL, 2μg/μL. Heterozygous knockout Species Packaging Human 1 vial parental cell Lysate and 1 vial knockout cell Lysate Gene ID Shipping Conditions 8976 4℃ Swiss Prot Application O00401 Western Blot Synonyms Storage N-WASP, NWASP, WASPB Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles. Protocol Contact To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). 400-999-6126 Lysate should be boiled for 3 - 5 minutes before loading onto gel. [email protected] www.abclonal.com.cn Sequencing data Genome sequence analysis of PCR products from parental (WT) and WASL knockout (KO) HeLa cells, using sanger sequencing.
    [Show full text]
  • Datasheet A05438-3 Anti-WASL Antibody
    Product datasheet Anti-WASL Antibody Catalog Number: A05438-3 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-WASL Antibody Gene Name WASL Source Rabbit IgG Species Reactivity human, mouse, rat Tested Application WB,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human N WASP/WASL recombinant protein (Position: Q5-H211). Purification Immunogen affinity purified. Observed MW 70KD Dilution Ratios Western blot: 1:500-2000 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. Reference Anti-WASL Antibody被引用在0文献中。 暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE.
    [Show full text]
  • The RNA-Protein Interactome of Differentiated Kidney Tubular Epithelial Cells
    BASIC RESEARCH www.jasn.org The RNA-Protein Interactome of Differentiated Kidney Tubular Epithelial Cells Michael Ignarski,1 Constantin Rill,1 Rainer W.J. Kaiser,1 Madlen Kaldirim,1 René Neuhaus,1 Reza Esmaillie,1 Xinping Li,2 Corinna Klein,3 Katrin Bohl,1 Maike Petersen,1 Christian K. Frese,3 Martin Höhne ,1 Ilian Atanassov,2 Markus M. Rinschen,1 Katja Höpker,1 Bernhard Schermer,1,4,5 Thomas Benzing,1,4,5 Christoph Dieterich,6,7 Francesca Fabretti,1 and Roman-Ulrich Müller 1,4,5 1Department II of Internal Medicine and Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital of Cologne, Cologne, Germany; 2Proteomics Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany; 3Proteomics Facility, Cologne Excellence Cluster on Cellular Stress Responses in Aging-associated Diseases, 4Nephrolab, Cologne Excellence Cluster on Cellular Stress Responses in Aging- associated Diseases, Faculty of Medicine and University Hospital Cologne, and 5Systems Biology of Ageing Cologne, University of Cologne, Cologne, Germany; 6Department of Internal Medicine III, Klaus Tschira Institute for Integrative Computational Cardiology, University Hospital Heidelberg, Heidelberg, Germany; and 7German Center for Cardiovascular Research (DZHK)–Partner site, Heidelberg/Mannheim, Germany ABSTRACT Background RNA-binding proteins (RBPs) are fundamental regulators of cellular biology that affect all steps in the generation and processing of RNA molecules. Recent evidence suggests that regulation of RBPs that modulate both RNA stability and translation may have a profound effect on the proteome. However, regulation of RBPs in clinically relevant experimental conditions has not been studied systematically. Methods We used RNA interactome capture, a method for the global identification of RBPs to characterize the global RNA‐binding proteome (RBPome) associated with polyA-tailed RNA species in murine ciliated epithelial cells of the inner medullary collecting duct.
    [Show full text]
  • Anti-WASL Antibody (ARG59532)
    Product datasheet [email protected] ARG59532 Package: 100 μl anti-WASL antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes WASL Tested Reactivity Ms, Rat Tested Application WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name WASL Antigen Species Human Immunogen KLH-conjugated synthetic peptide between aa. 165-198 of Human WASL. Conjugation Un-conjugated Alternate Names WASPB; N-WASP; NWASP; Neural Wiskott-Aldrich syndrome protein Application Instructions Application table Application Dilution WB 1:1000 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control C2C12 Calculated Mw 55 kDa Properties Form Liquid Purification Purification with Protein A and immunogen peptide. Buffer PBS and 0.09% (W/V) Sodium azide. Preservative 0.09% (W/V) Sodium azide. Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation www.arigobio.com 1/2 Gene Symbol WASL Gene Full Name Wiskott-Aldrich syndrome-like Background This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex.
    [Show full text]
  • WASL Polyclonal Antibody
    PRODUCT DATA SHEET Bioworld Technology,Inc. WASL polyclonal antibody Catalog: BS91449 Host: Rabbit Reactivity: Human, Mouse BackGround: Storage&Stability: This gene encodes a member of the Wiskott-Aldrich syn- Store at +4°C after thawing. Aliquot store at -20°C or drome (WAS) protein family. Wiskott-Aldrich syndrome -80°C. Avoid repeated freeze / thaw cycles. proteins share similar domain structure, and associate Specificity: with a variety of signaling molecules to alter the actin WASL polyclonal antibody detects endogenous levels of cytoskeleton. The encoded protein is highly expressed in WASL protein. neural tissues, and interacts with several proteins in- DATA: volved in cytoskeletal organization, including cell divi- sion control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to Western blot analysis of WASL on mouse Human serum lysates using HSF1/HSTF1 and forms a complex on heat shock pro- anti-Ubiquitin antibody at 1/500 dilution. moter elements (HSE) that negatively regulates HSP90 expression.. Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Molecular Weight: 55 kDa Swiss-Prot: ICC staining WASL in SK-Br-3 cells (green). The nuclear counter stain O00401(Human) Q91YD9(Mouse) is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised Purification&Purity: with 0.25% Triton X100/PBS.
    [Show full text]
  • 4036.Full.Pdf
    The Journal of Immunology Disruption of Thrombocyte and T Lymphocyte Development by a Mutation in ARPC1B Raz Somech,*,† Atar Lev,*,† Yu Nee Lee,*,† Amos J. Simon,*,†,‡ Ortal Barel,†,x Ginette Schiby,{ Camila Avivi,{ Iris Barshack,{ Michele Rhodes,‖ Jiejing Yin,‖ Minshi Wang,‖ Yibin Yang,‖ Jennifer Rhodes,‖ Nufar Marcus,# Ben-Zion Garty,# Jerry Stein,** Ninette Amariglio,†,‡,x,†† Gideon Rechavi,†,x David L. Wiest,‖,1 and Yong Zhang‖,1 Regulation of the actin cytoskeleton is crucial for normal development and function of the immune system, as evidenced by the severe immune abnormalities exhibited by patients bearing inactivating mutations in the Wiskott–Aldrich syndrome protein (WASP), a key regulator of actin dynamics. WASP exerts its effects on actin dynamics through a multisubunit complex termed Arp2/3. Despite the critical role played by Arp2/3 as an effector of WASP-mediated control over actin polymerization, mutations in protein components of the Arp2/3 complex had not previously been identified as a cause of immunodeficiency. Here, we describe two brothers with hematopoietic and immunologic symptoms reminiscent of Wiskott–Aldrich syndrome (WAS). However, these patients lacked mutations in any of the genes previously associated with WAS. Whole-exome sequencing revealed a homozygous 2 bp deletion, n.c.G623DEL-TC (p.V208VfsX20), in Arp2/3 complex component ARPC1B that causes a frame shift resulting in premature termination. Modeling of the disease in zebrafish revealed that ARPC1B plays a critical role in supporting T cell and thrombocyte development. Moreover, the defects in development caused by ARPC1B loss could be rescued by the intact human ARPC1B ortholog, but not by the p.V208VfsX20 variant identified in the patients.
    [Show full text]
  • Staphylococcus Aureus Recruits Cdc42gap Through Recycling
    © 2016. Published by The Company of Biologists Ltd | Journal of Cell Science (2016) 129, 2937-2949 doi:10.1242/jcs.186213 RESEARCH ARTICLE Staphylococcus aureus recruits Cdc42GAP through recycling endosomes and the exocyst to invade human endothelial cells Liane Rauch1,*, Kirsten Hennings1,*, Claudia Trasak1, Anja Röder1, Barbara Schröder2,3, Friedrich Koch-Nolte4, Felix Rivera-Molina5, Derek Toomre5 and Martin Aepfelbacher1,‡ ABSTRACT Staphylococcus aureus is a major agent of blood stream infection Activation and invasion of the vascular endothelium by and sepsis worldwide (Lowy, 1998). Activation and invasion of the Staphylococcus aureus is a major cause of sepsis and vascular endothelium is thought to underlie the main symptoms of S. aureus endocarditis. For endothelial cell invasion, S. aureus triggers actin sepsis (Kerrigan and McDonnell, 2015). Furthermore, S. aureus polymerization through Cdc42, N-WASp (also known as WASL) and has a propensity to invade the endothelial lining of heart the Arp2/3 complex to assemble a phagocytic cup-like structure. valves leading to valve colonization and bacterial endocarditis Here, we show that after stimulating actin polymerization (Chorianopoulos et al., 2009). Animal models have revealed that S. aureus staphylococci recruit Cdc42GAP (also known as ARHGAP1) which intravascular preferentially attaches to the endothelium of deactivates Cdc42 and terminates actin polymerization in the postcapillary venules (Laschke et al., 2005). In vitro in vivo S. aureus phagocytic cups. Cdc42GAP is delivered to the invading bacteria and invades endothelial cells through its on recycling endocytic vesicles in concert with the exocyst complex. surface-exposed fibronectin-binding proteins A and B (FnBPA and When Cdc42GAP recruitment by staphylococci was prevented by FnBPB) (Que et al., 2005; Schroder et al., 2006; Sinha et al., 2000).
    [Show full text]
  • Integration of Protein Binding Interfaces and Abundance
    INTEGRATION OF PROTEIN BINDING INTERFACES AND ABUNDANCE DATA REVEALS EVOLUTIONARY PRESSURES IN PROTEIN NETWORKS by David Orestis Holland A dissertation submitted to Johns Hopkins University in conformity with the requirements for the degree of Doctor of Philosophy. Baltimore, Maryland January, 2018 © David O. Holland 2018 All rights reserved Abstract Networks of protein-protein interactions have received considerable interest in the past two decades for their insights about protein function and evolution. Traditionally, these networks only map the functional partners of proteins; they lack further levels of data such as binding affinity, allosteric regulation, competitive vs noncompetitive binding, and protein abundance. Recent experiments have made such data on a network-wide scale available, and in this thesis I integrate two extra layers of data in particular: the binding sites that proteins use to interact with their partners, and the abundance or “copy numbers” of the proteins. By analyzing the networks for the clathrin-mediated endocytosis (CME) system in yeast and the ErbB signaling pathway in humans, I find that this extra data reveals new insights about the evolution of protein networks. The structure of the binding site or interface interaction network (IIN) is optimized to allow higher binding specificity; that is, a high gap in strength between functional binding and nonfunctional mis-binding. This strongly implies that mis-binding is an evolutionary error-load constraint shaping protein network structure. Another method to limit mis-binding is to balance protein copy numbers so that there are no “leftover” proteins available for mis-binding. By developing a new method to quantify balance in IINs, I show that ii the CME network is significantly balanced when compared to randomly sampled sets of copy numbers.
    [Show full text]
  • A Proximity-Labeling Proteomic Approach to Investigate
    A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells Sylvie Thuault, Claire Mamelonet, Joëlle Salameh, Kevin Ostacolo, Brice Chanez, Danièle Salaün, Emilie Baudelet, Stéphane Audebert, Luc Camoin, Ali Badache To cite this version: Sylvie Thuault, Claire Mamelonet, Joëlle Salameh, Kevin Ostacolo, Brice Chanez, et al.. A proximity- labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci- entific Reports, Nature Publishing Group, 2020, 10 (1), 10.1038/s41598-020-63926-4. hal-03174369 HAL Id: hal-03174369 https://hal-amu.archives-ouvertes.fr/hal-03174369 Submitted on 30 Mar 2021 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License www.nature.com/scientificreports OPEN A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells Sylvie Thuault1 ✉ , Claire Mamelonet1, Joëlle Salameh1,3, Kevin Ostacolo1,4, Brice Chanez1,5, Danièle Salaün1, Emilie Baudelet2, Stéphane Audebert2, Luc Camoin2 & Ali Badache1 Metastatic progression is the leading cause of mortality in breast cancer. Invasive tumor cells develop invadopodia to travel through basement membranes and the interstitial matrix. Substantial eforts have been made to characterize invadopodia molecular composition.
    [Show full text]