Datasheet A05438-3 Anti-WASL Antibody

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Product datasheet Anti-WASL Antibody Catalog Number: A05438-3 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-WASL Antibody Gene Name WASL Source Rabbit IgG Species Reactivity human, mouse, rat Tested Application WB,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human N WASP/WASL recombinant protein (Position: Q5-H211). Purification Immunogen affinity purified. Observed MW 70KD Dilution Ratios Western blot: 1:500-2000 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. Reference Anti-WASL Antibody被引用在0文献中。暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-WASL Antibody Catalog Number: A05438-3 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot Figure 2. Flow cytometry analysis of anti- WASL analysis of HELA cells antibody (A05438-3). The (1x106) DyLight 488 sample well of each lane conjugated goat anti- was loaded with 50ug of rabbit IgG(blue) was used sample under reducing as secondary conditions. Lane 1: antibody.Isotype control human SH-SY5Y whole antibody (Green line) was cell lysates, Lane 2: rabbit IgG DyLight 488. human U87 whole cell Unlabelled sample (Red lysates, Lane 3: human line). HELA whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat liver tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysayes, Lane 10: mouse lung tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse Neuro-2a whole cell lysates. Use rabbit anti- WASL 1:1000, probed with a goat anti- rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for WASL at approximately 70KD. The expected band size for WASL at 70KD. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Powered by TCPDF (www.tcpdf.org).

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