Product datasheet Anti-WASL Antibody Catalog Number: A05438-3

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-WASL Antibody Name WASL Source Rabbit IgG Species Reactivity human, mouse, rat Tested Application WB,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human N WASP/WASL recombinant (Position: Q5-H211). Purification Immunogen affinity purified. Observed MW 70KD Dilution Ratios Western blot: 1:500-2000 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Neural Wiskott-Aldrich syndrome protein is a protein that in humans is encoded by the WASL gene. This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome share similar domain structure, and associate with a variety of signaling molecules to alter the . The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-WASL Antibody Catalog Number: A05438-3

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. Flow cytometry analysis of anti- WASL analysis of HELA cells antibody (A05438-3). The (1x106) DyLight 488 sample well of each lane conjugated goat anti- was loaded with 50ug of rabbit IgG(blue) was used sample under reducing as secondary conditions. Lane 1: antibody.Isotype control human SH-SY5Y whole antibody (Green line) was cell lysates, Lane 2: rabbit IgG DyLight 488. human U87 whole cell Unlabelled sample (Red lysates, Lane 3: human line). HELA whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat liver tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysayes, Lane 10: mouse lung tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse Neuro-2a whole cell lysates. Use rabbit anti- WASL 1:1000, probed with a goat anti- rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for WASL at approximately 70KD. The expected band size for WASL at 70KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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