서식처 유형에 따른 배스 Micropterus Salmoides 식성의 차이
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Characterization, Expression Profile, and Promoter Analysis of the Rhodeus Uyekii Vitellogenin Ao1 Gene
Int. J. Mol. Sci. 2014, 15, 18804-18818; doi:10.3390/ijms151018804 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Article Characterization, Expression Profile, and Promoter Analysis of the Rhodeus uyekii Vitellogenin Ao1 Gene Hee Jeong Kong 1, Ju Lan Kim 1, Ji Young Moon 1, Woo-Jin Kim 1, Hyung Soo Kim 1, Jung Youn Park 1, Hyun Kook Cho 2,* and Cheul Min An 1 1 Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea; E-Mails: [email protected] (H.J.K.); [email protected] (J.L.K.); [email protected] (J.Y.M.); [email protected] (W.-J.K.); [email protected] (H.S.K.); [email protected] (J.Y.P.); [email protected] (C.M.A.) 2 Department of Molecular Biology, Pusan National University, Busan 609-735, Korea * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +82-51-510-3671; Fax: +82-51-513-9258. External Editor: Xiaofeng Jia Received: 21 July 2014; in revised form: 24 September 2014 / Accepted: 11 October 2014 / Published: 17 October 2014 Abstract: The fish Vitellogenin (Vg) gene has been applied as a biomarker for exposure to estrogenic compounds in the aquatic environment. In this study, we cloned and characterized Vg cDNA from the Korean rose bitterling Rhodeus uyekii (Ru-Vg). The Ru-Vg cDNA encodes a 1424-amino-acid polypeptide that belongs to the VgAo1 family and contains a putative signal peptide, lipovitellin I, phosvitin, and lipovitellin II, but does not contain the vWFD domain or the C-terminal peptide. -
Sexual Selection for Male Dominance Reduces Opportunities for Female Mate Choice in the European Bitterling (Rhodeus Sericeus)
Molecular Ecology (2005) 14, 1533–1542 doi: 10.1111/j.1365-294X.2005.02534.x SexualBlackwell Publishing, Ltd. selection for male dominance reduces opportunities for female mate choice in the European bitterling (Rhodeus sericeus) M. REICHARD,* J. BRYJA,* M. ONDRA3KOVÁ,* M. DÁVIDOVÁ,† P. KANIEWSKA‡ and C. SMITH§ *Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno, Czech Republic; †Department of Zoology and Ecology, Faculty of Science, Masaryk University, Brno, Czech Republic; ‡Centre for Marine Studies, University of Queensland, Brisbane, Queensland 4072, Australia; §Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom Abstract Sexual selection involves two main mechanisms: intrasexual competition for mates and intersexual mate choice. We experimentally separated intrasexual (male–male interference competition) and intersexual (female choice) components of sexual selection in a freshwater fish, the European bitterling (Rhodeus sericeus). We compared the roles of multiple morphological and behavioural traits in male success in both components of sexual com- petition, and their relation to male reproductive success, measured as paternity of off- spring. Body size was important for both female choice and male–male competition, though females also preferred males that courted more vigorously. However, dominant males often monopolized females regardless of female preference. Subordinate males were not excluded from reproduction and sired some offspring, possibly through sneaked ejaculations. Male dominance and a greater intensity of carotenoid-based red colouration in their iris were the best predictors of male reproductive success. The extent of red iris colouration and parasite load did not have significant effects on female choice, male dominance or male reproductive success. -
Rhodeus Uyekii)
Dev. Reprod. Vol. 18, No. 4, 251~258, December, 2014 http://dx.doi.org/10.12717/DR.2014.18.4.251 ISSN 1226-6752 (Print) ISSN 2287-7967 (Online) Fish Myogenic Regulatory Protein LUC7L: Characterization and Expression Analysis in Korean Rose Bitterling (Rhodeus uyekii) Ju Lan Kim, †Hee Jeong Kong, Hyung Soo Kim, Woo-Jin Kim, Dong-Gyun Kim, Bo-Hye Nam, Young-Ok Kim and Cheul Min An Biotechnology Research Division, National Fisheries Research and Development Institute, Busan 619-705, Korea ABSTRACT : Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii. Key words : Development, Korean rose bitterling, Luc7l, Myogenesis, Rhodeus uyekii INTRODUCTION there are several common features in muscle development between teleost and mammals, myogenesis in teleost has Myogenesis (or muscle development) is composed of some unique features compared to mammals, which include the specification processes of the individual myogenic the early stage of muscle commitment, presence of adaxial progenitor cells according to myogenic lineage, proliferation cells, different proportions of slow and fast fibers, and and migration, differentiation, and fusion. -
Dong-Kyun KIM1, 2, Hyunbin JO1, Wan-Ok LEE1, Kiyun PARK1, and Ihn-Sil KWAK*1, 3
ACTA ICHTHYOLOGICA ET PISCATORIA (2020) 50 (2): 209–213 DOI: 10.3750/AIEP/02790 EVALUATION OF LENGTH–WEIGHT RELATIONS FOR 15 FISH SPECIES (ACTINOPTERYGII) FROM THE SEOMJIN RIVER BASIN IN SOUTH KOREA Dong-Kyun KIM1, 2, Hyunbin JO1, Wan-Ok LEE1, Kiyun PARK1, and Ihn-Sil KWAK*1, 3 1 Fisheries Science Institute, Chonnam National University, Yeosu, Republic of Korea 2 K-water Research Institute, Daejeon, Republic of Korea 3 Faculty of Marine Technology, Chonnam National University, Yeosu, Republic of Korea Kim D.-K., Jo H., Lee W.-O., Park K., Kwak I.-S. 2020. Evaluation of length–weight relations for 15 fish species (Actinopterygii) from the Seomjin River basin in South Korea. Acta Ichthyol. Piscat. 50 (2): 209–213. Abstract. This study demonstrates the estimation of length–weight relations (LWR) for freshwater fishes from the Seomjin River basin in South Korea. The LWR estimation is based on the 15 species representing Cyprinidae: Rhodeus uyekii (Mori, 1935), Rhodeus notatus Nichols, 1929, Tanakia koreensis (Kim et Kim, 1990), Acheilognathus rhombeus (Temminck et Schlegel, 1846), Pseudorasbora parva (Temminck et Schlegel, 1846), Coreoleuciscus aeruginos Song et Bang, 2015, Sarcocheilichthys nigripinnis (Günther, 1873), Squalidus gracilis majimae (Jordan et Hubbs, 1925), Squalidus chankaensis tsuchigae (Jordan et Hubbs, 1925), Hemibarbus longirostris (Regan, 1908), and Opsariichthys uncirostris (Temminck et Schlegel, 1846); Cobitidae: Cobitis longicorpus Kim, Choi et Nalbant, 1976 and Cobitis tetralineata (Kim, Park et Nalbant, 1999); Bagridae: Tachysurus ussuriensis (Dybowski, 1872); and Amblycipidae: Liobagrus somjinensis Park et Kim, 2011. Our study provides new information of LWRs for eight species. The LWRs for those species have not been reported yet in FishBase. -
Globally Important Agricultural Heritage Systems (GIAHS) Application
Globally Important Agricultural Heritage Systems (GIAHS) Application SUMMARY INFORMATION Name/Title of the Agricultural Heritage System: Osaki Kōdo‟s Traditional Water Management System for Sustainable Paddy Agriculture Requesting Agency: Osaki Region, Miyagi Prefecture (Osaki City, Shikama Town, Kami Town, Wakuya Town, Misato Town (one city, four towns) Requesting Organization: Osaki Region Committee for the Promotion of Globally Important Agricultural Heritage Systems Members of Organization: Osaki City, Shikama Town, Kami Town, Wakuya Town, Misato Town Miyagi Prefecture Furukawa Agricultural Cooperative Association, Kami Yotsuba Agricultural Cooperative Association, Iwadeyama Agricultural Cooperative Association, Midorino Agricultural Cooperative Association, Osaki Region Water Management Council NPO Ecopal Kejonuma, NPO Kabukuri Numakko Club, NPO Society for Shinaimotsugo Conservation , NPO Tambo, Japanese Association for Wild Geese Protection Tohoku University, Miyagi University of Education, Miyagi University, Chuo University Responsible Ministry (for the Government): Ministry of Agriculture, Forestry and Fisheries The geographical coordinates are: North latitude 38°26’18”~38°55’25” and east longitude 140°42’2”~141°7’43” Accessibility of the Site to Capital City of Major Cities ○Prefectural Capital: Sendai City (closest station: JR Sendai Station) ○Access to Prefectural Capital: ・by rail (Tokyo – Sendai) JR Tohoku Super Express (Shinkansen): approximately 2 hours ※Access to requesting area: ・by rail (closest station: JR Furukawa -
Amur Fish: Wealth and Crisis
Amur Fish: Wealth and Crisis ББК 28.693.32 Н 74 Amur Fish: Wealth and Crisis ISBN 5-98137-006-8 Authors: German Novomodny, Petr Sharov, Sergei Zolotukhin Translators: Sibyl Diver, Petr Sharov Editors: Xanthippe Augerot, Dave Martin, Petr Sharov Maps: Petr Sharov Photographs: German Novomodny, Sergei Zolotukhin Cover photographs: Petr Sharov, Igor Uchuev Design: Aleksey Ognev, Vladislav Sereda Reviewed by: Nikolai Romanov, Anatoly Semenchenko Published in 2004 by WWF RFE, Vladivostok, Russia Printed by: Publishing house Apelsin Co. Ltd. Any full or partial reproduction of this publication must include the title and give credit to the above-mentioned publisher as the copyright holder. No photographs from this publication may be reproduced without prior authorization from WWF Russia or authors of the photographs. © WWF, 2004 All rights reserved Distributed for free, no selling allowed Contents Introduction....................................................................................................................................... 5 Amur Fish Diversity and Research History ............................................................................. 6 Species Listed In Red Data Book of Russia ......................................................................... 13 Yellowcheek ................................................................................................................................... 13 Black Carp (Amur) ...................................................................................................................... -
Evaluación Del Gen (3-Actina En Arapaima Gigas
UNIVERSIDAD NACIONAL DE PIURA FACULTAD DE CIENCIAS ESCUELA PROFESIONAL DE CIENCIAS BIOLÓGICAS "Evaluación del gen (3-actina en Arapaima gigas "paiche" como control interno de PCR en tiempo real (qPCR)" Br. DANIELA GONZÁLEZ BARDALES TESIS PARA OPTAR EL TÍTULO PROFESIONAL DE BIÓLOGO Piura- Perú 2013 Br. DANIELA GONZÁLEZ BARDALES AUTORA Blgo - Mcblg JN FERNÁNDEZ PONCE ESOR Blgo - Mcblgo. CÉSA TORRES DÍAZ M.Sc COA SOR 721 - Dr. ROBERTO MENDOZA RENDON Blgo. ROBERT BARRIONUEVO GARCÍA PRESIDENTE SECRETARIO Blgo. RONALD MARCIAL RAMOS VOCAL A mi madre: 'Por todo el apoyo y el aliento brindado en los momentos de debilidad. ÍNDICE GENERAL Pág. INTRODUCCIÓN 1 MATERIAL Y MÉTODOS 12 Obtención de la muestra. 12 Identificación del gen I3-actina 12 Extracción de ARN total de tejido. 12 Transcripción reversa-PCR (RT-PCR). 13 Reacción en cadena de la polimerasa (PCR). 14 Secuenciación. 15 Análisis de secuencias e identificación del gen. 15 Evaluación del gen I3-actina 15 Purificación de productos de PCR. 15 Ligación del plásmido. 17 Competencia bacteriana. 18 Transformación de bacterias competentes. 19 Evaluación de bacterias transformadas. 19 Extracción de ADN plasmídico. 20 Primocultivos de células sanguíneas en A. gigas "paiche". 22 Inducción con lipopolísacáridos (LPS). 22 Extracción de ARN total a partir de cultivos de células sanguíneas 23 Reacción en cadena de la polimerasa en tiempo real (qPCR) 24 Creación de la curva estándar 25 RESULTADOS 26 Reacción en cadena de la polimerasa (PCR). 26 Análisis de secuencia e identificación del gen- 28 Transformación de bacterias competentes y obtención del ADN plasmídico- 30 PCR en tiempo real (qPCR). 32 DISCUSIÓN 35 CONCLUSIONES 38 REFERENCIAS BIBLIOGRÁFICAS 39 ANEXOS ÍNDICE DE FIGURAS Pág. -
옥정호에 도입된 배스 Micropterus Salmoides의 식성 및 어류상에
KOREAN JOURNAL OF ICHTHYOLOGY, Vol. 20, No. 1, 36-44, March 2008 Received : January 23, 2008 ISSN: 1225-8598 Accepted : March 10, 2008 옥정호에 도입된 배스 Micropterus salmoides의식성및 어류상에 미치는 영향 고명훈∙박종영*∙이용주1 전북대학교 자연과학대학 생물과학부, 1전주교육대학교 과학교육학과 Feeding Habits of an Introduced Large Mouth Bass, Micropterus salmoides (Perciformes; Centrachidae), and Its Influence on Ichthyofauna in the Lake Okjeong, Korea by Myeong-Hun Ko, Jong-Young Park* and Yong-Joo Lee1 (Faculty of Biological Science, College of Natural Science, Chonbuk National University, Jeonju 561-756, Korea; 1Department of Science Education, Jeonju National University of Education, Jeonju 560-757, Korea) ABSTRACT Feeding habits of Micropterus salmoides were investigated from the stomach contents of specimens ranging from 13 to 511 mm in standard length (n=419), which are collected in the Lake Okjeong, Korea, 2006 to 2007. M. salmoides is a very strong carnivorous fish eating living organisms such as lots of fishes, small amount of aquatic insects and shrimps. As they grow, the feeding habits changed : they fed mainly on Branchioda in 10 to 20 mm in SL., aquatic insects and small fishes in 30 to 50 mm in SL., fishes including Zacco platypus, Hemiculter eigenmanni and Rhinogobius brunneus over 50 mm. However, among the prey organisms, M. salmoides most preferred living fishes. Analy- sing ichthyofauna of the Lake Okjeong from 1981 to the present investigation, after and before intro- duction of M. salmoides, it was showed that there was a certain reduction in fish diversity. Consequent- ly, it is likely to let the strongly fish-eating habit to reduce a diversity of native fishes in the lake. -
MASARYKOVA UNIVERZITA Analýza Vybraných Ekologických a Evolučně-Behaviorálních Aspektů V Systému Parazit-Hostitel
MASARYKOVA UNIVERZITA Přírodovědecká fakulta Martina DÁVIDOVÁ Analýza vybraných ekologických a evolučně-behaviorálních aspektů v systému parazit-hostitel Disertační práce Školitel: doc. RNDr. Milan Gelnar, CSc. Brno, 2008 Bibliografická identifikace Jméno a příjmení autora: Martina Dávidová Název disertační práce: Analýza vybraných ekologických a evolučně-behaviorálních aspektů v systému parazit-hostitel Název disertační práce anglicky: Analysis of selected ecological and evolutionary-behavioural aspects of host-parasite systems Studijní program: Biologie Studijní obor (směr), kombinace oborů: Parazitologie Školitel: doc. RNDr. Milan Gelnar, CSc. Rok obhajoby: 2008 Klíčová slova v češtině: systém parazit-rybí hostitel, interakce, biotické a abiotické faktory, virulence, selektivní predace, pohlavní výběr Klíčová slova v angličtině: host fish-parasite system, interactions, biotic and abiotic factors, virulence, selective predation, sexual selection © Martina Dávidová, Masarykova univerzita, Brno, 2008 Poděkování Mé poděkování patří mému školiteli doc. RNDr. Milanu Gelnarovi, CSc. za poskytnutí zázemí, rad, trpělivosti a finanční podpory při zpracování této práce. Můj velký dík určitě patří Andree Šimkové, Markétě Ondračkové a Radimu Blažkovi za jejich podporu, pomoc a optimismus v každé situaci; Jirkovi Jarkovskému, Honzovi Mužíkovi a Katce Houdkové za pomoc při řešení statistických zapeklitostí; Ivetě Matějusové za korektury anglicky psaného textu; Pavlu Jurajdovi, Martinu Reichardovi, Michalu Janáčovi a Zdeničce Valové za jejich neocenitelnou pomoc v terénu a také všem z oddělení parazitologie, kteří se jakýmkoliv způsobem podíleli na sběru dat. Prof. Arnemu Skorpingovi děkuji za možnost vyzkoušet si „něco jiného“ na univerzitě v norském Bergenu. Především děkuji své rodině a přátelům za podporu a pochopení, které jsem často potřebovala. Abstrakt Studium vybraných interakcí v systému parazit-hostitel (model: ryba-mnohobuněčný cizopasník) bylo ústředním tématem předložené disertační práce. -
Seung-Ho BAEK 1, 2, Sang-Hyeon PARK 1, 3, Jeong-Suk MOON 4, and Jeong-Hui KIM*1
ACTA ICHTHYOLOGICA ET PISCATORIA (2020) 50 (2): 215–217 DOI: 10.3750/AIEP/02850 LENGTH–WEIGHT RELATIONS FOR 16 FRESHWATER FISH SPECIES (ACTINOPTERYGII: CYPRINIFORMES: CYPRINIDAE) IN THE HAN RIVER, SOUTH KOREA Seung-Ho BAEK 1, 2, Sang-Hyeon PARK 1, 3, Jeong-Suk MOON 4, and Jeong-Hui KIM *1 1 EcoResearch incorporated, Gongju, South Korea 2 Department of Environmental Engineering, Chungbuk National University, Cheongju, South Korea 3 Department of Marine Fisheries Resources, Mokpo National University, Mokpo, South Korea 4 National Institute of Environmental Research, Incheon, South Korea Baek S.-H., Park S.-H., Moon J.-S., Kim J.-H. 2020. Length–weight relations for 16 freshwater fish species in the Han River, South Korea. Acta Ichthyol. Piscat. 50 (2): 215–217. Abstract. Length–weight relations (LWRs) for 16 freshwater fish collected from June to October 2018 in the Han River, South Korea, were estimated. The following species, representing the family Cyprinidae, were studied: Abbottina rivularis (Basilewsky, 1855); Acanthorhodeus chankaensis (Dybowski, 1872); Tanakia lanceolata (Temminck et Schlegel, 1846); Acheilognathus rhombeus (Temminck et Schlegel, 1846); Acheilognathus yamatsutae Mori, 1928; Carassius auratus (Linnaeus, 1758); Chanodichthys erythropterus (Basilewsky, 1855); Gnathopogon strigatus (Regan, 1908); Hemibarbus labeo (Pallas, 1776); Pseudogobio esocinus (Temminck et Schlegel, 1846); Pseudorasbora parva (Temminck et Schlegel, 1846); Pungtungia herzi Herzenstein, 1892; Sarcocheilichthys nigripinnis (Günther, 1873); Squalidus chankaensis Dybowski, 1872; Squalidus japonicus (Sauvage, 1883); Opsariichthys uncirostris (Temminck et Schlegel, 1846). The values of parameter b for all species were within the expected 2.5–3.5 range according to Froese (2006). The LWRs data for two species in this study, Acheilognathus rhombeus and Gnathopogon strigatus, were not previously available in FishBase. -
Cyprinidae: Acheilognathinae) from Japan
Bull. Natl. Mus. Nat. Sci., Ser. A, Suppl. 1, pp. 1–28, March 22, 2007 Four New Subspecies of Acheilognathus Bitterlings (Cyprinidae: Acheilognathinae) from Japan Ryoichi Arai1, Hiroshi Fujikawa2 and Yoshikazu Nagata3 1Department of Zoology, University Museum, University of Tokyo, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan E-mail: [email protected] 2Osaka Prefectural Board of Education, Chuo-ku, Osaka 540–8571, Japan 3Department of Biology, Osaka Kyoiku University, 4–698–1 Asahigaoka, Kashiwara, Osaka 582–8582, Japan Abstract Four new bitterlings, Acheilognathus tabira erythropterus subsp. nov., Acheilognathus tabira tohokuensis subsp. nov., Acheilognathus tabira jordani subsp. nov. and Acheilognathus tabi- ra nakamurae subsp. nov., were described on the basis of more than 600 specimens from 26 locali- ties in Japan. Acheilognathus tabira erythropterus, A. t. tohokuensis and A. t. jordani, formerly all included in a single undescribed subspecies of Acheilognathus tabira, differ from other subspecies of A. tabira in having a red-edged anal fin in nuptial males. Acheilognathus t. erythropterus, dis- tributed on the Pacific Ocean side of eastern Honshu, is distinguished from A. t. tohokuensis and A. t. jordani by having shorter ellipsoidal eggs (ratio of major axis to minor axis: 1.4–2.2 vs. 2.0–3.3 in A. t. tohokuensis and A. t. jordani). Acheilognathus t. tohokuensis, distributed on the Japan Sea side of eastern Honshu, is distinguished from A. t. jordani in lacking a black blotch on the dorsal fin in juveniles. Acheilognathus t. jordani, distributed on the Japan Sea side of western Honshu, is distinguished from A. -
André Phillips Phd Thesis
THE MECHANISMS AND CONSEQUENCES OF OVIPOSITION DECISIONS IN THE EUROPEAN BITTERLING André Phillips A Thesis Submitted for the Degree of PhD at the University of St Andrews 2018 Full metadata for this item is available in St Andrews Research Repository at: http://research-repository.st-andrews.ac.uk/ Please use this identifier to cite or link to this item: http://hdl.handle.net/10023/15524 This item is protected by original copyright The mechanisms and consequences of oviposition decisions in the European bitterling André Phillips 1. Candidate’s declarations: I, André Phillips hereby certify that this thesis, which is approximately 38,000 words in length, has been written by me, and that it is the record of work carried out by me, or principally by myself in collaboration with others as acknowledged, and that it has not been submitted in any previous application for a higher degree. I was admitted as a research student in October, 2014 and as a candidate for the degree of PhD in October, 2014; the higher study for which this is a record was carried out in the University of St Andrews between 2014 and 2017. I, André Phillips, received assistance in the writing of this thesis in respect of grammar & spelling, which was provided by Helen Spence-Jones Date …… signature of candidate ……… 2. Supervisor’s declaration: I hereby certify that the candidate has fulfilled the conditions of the Resolution and Regulations appropriate for the degree of ……… in the University of St Andrews and that the candidate is qualified to submit this thesis in application for that degree.