Recombinant Human ARFIP2 Protein Catalog Number: ATGP1695

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Recombinant human ARFIP2 protein Catalog Number: ATGP1695 PRODUCT INPORMATION Expression system E.coli Domain 1-341aa UniProt No. P53365 NCBI Accession No. NP_036534 Alternative Names Arfaptin 2, POR1 PRODUCT SPECIFICATION Molecular Weight 40.2 kDa (364aa) confirmed by MALDI-TOF Concentration 0.25mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 1mM DTT Purity > 90% by SDS-PAGE Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description Arfaptin 2, also known as ARFIP2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. ARFIP2 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. Arfaptin 2 also regulates the aggregation of mutant Huntingtin protein by possibly impairing proteasome function. Expression of ARFIP2 was shown to be increased at sites of neurodegeneration. Recombinant human ARFIP2 protein, fused to His-tag at N-terminus, was expressed in E. coli 1 Recombinant human ARFIP2 protein Catalog Number: ATGP1695 and purified by using conventional chromatography techniques. Amino acid Sequence MGSSHHHHHH SSGLVPRGSH MGSMTDGILG KAATMEIPIH GNGEARQLPE DDGLEQDLQQ VMVSGPNLNE TSIVSGGYGG SGDGLIPTGS GRHPSHSTTP SGPGDEVARG IAGEKFDIVK KWGINTYKCT KQLLSERFGR GSRTVDLELE LQIELLRETK RKYESVLQLG RALTAHLYSL LQTQHALGDA FADLSQKSPE LQEEFGYNAE TQKLLCKNGE TLLGAVNFFV SSINTLVTKT MEDTLMTVKQ YEAARLEYDA YRTDLEELSL GPRDAGTRGR LESAQATFQA HRDKYEKLRG DVAIKLKFLE ENKIKVMHKQ LLLFHNAVSA YFAGNQKQLE QTLQQFNIKL RPPGAEKPSW LEEQ General References D'Souza Schorey C., et al. (1997) EMBO J. 16: 5445-5454 Joneson T., et al. (1996) Science. 274: 1374-1376. DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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Supplemental information Dissection of the genomic structure of the miR-183/96/182 gene. Previously, we showed that the miR-183/96/182 cluster is an intergenic miRNA cluster, located in a ~60-kb interval between the genes encoding nuclear respiratory factor-1 (Nrf1) and ubiquitin-conjugating enzyme E2H (Ube2h) on mouse chr6qA3.3 (1). To start to uncover the genomic structure of the miR- 183/96/182 gene, we first studied genomic features around miR-183/96/182 in the UCSC genome browser (http://genome.UCSC.edu/), and identified two CpG islands 3.4-6.5 kb 5’ of pre-miR-183, the most 5’ miRNA of the cluster (Fig. 1A; Fig. S1 and Seq. S1). A cDNA clone, AK044220, located at 3.2-4.6 kb 5’ to pre-miR-183, encompasses the second CpG island (Fig. 1A; Fig. S1). We hypothesized that this cDNA clone was derived from 5’ exon(s) of the primary transcript of the miR-183/96/182 gene, as CpG islands are often associated with promoters (2). Supporting this hypothesis, multiple expressed sequences detected by gene-trap clones, including clone D016D06 (3, 4), were co-localized with the cDNA clone AK044220 (Fig. 1A; Fig. S1). Clone D016D06, deposited by the German GeneTrap Consortium (GGTC) (http://tikus.gsf.de) (3, 4), was derived from insertion of a retroviral construct, rFlpROSAβgeo in 129S2 ES cells (Fig. 1A and C). The rFlpROSAβgeo construct carries a promoterless reporter gene, the β−geo cassette - an in-frame fusion of the β-galactosidase and neomycin resistance (Neor) gene (5), with a splicing acceptor (SA) immediately upstream, and a polyA signal downstream of the β−geo cassette (Fig.
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Entrez ID 1 Symbol 1 Entrez ID 2 Symbol 2 Data Source (R
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Supplementary Data
SUPPLEMENTAL INFORMATION A study restricted to chemokine receptors as well as a genome-wide transcript analysis uncovered CXCR4 as preferentially expressed in Ewing's sarcoma (Ewing's sarcoma) cells of metastatic origin (Figure 4). Transcriptome analyses showed that in addition to CXCR4, genes known to support cell motility and invasion topped the list of genes preferentially expressed in metastasis-derived cells (Figure 4D). These included kynurenine 3-monooxygenase (KMO), galectin-1 (LGALS1), gastrin-releasing peptide (GRP), procollagen C-endopeptidase enhancer (PCOLCE), and ephrin receptor B (EPHB3). KMO, a key enzyme of tryptophan catabolism, has not been linked to metastasis. Tryptophan and its catabolites, however, are involved in immune evasion by tumors, a process that can assist in tumor progression and metastasis (1). LGALS1, GRP, PCOLCE and EPHB3 have been linked to tumor progression and metastasis of several cancers (2-4). Top genes preferentially expressed in L-EDCL included genes that suppress cell motility and/or potentiate cell adhesion such as plakophilin 1 (PKP1), neuropeptide Y (NPY), or the metastasis suppressor TXNIP (5-7) (Figure 4D). Overall, L-EDCL were enriched in gene sets geared at optimizing nutrient transport and usage (Figure 4D; Supplementary Table 3), a state that may support the early stages of tumor growth. Once tumor growth outpaces nutrient and oxygen supplies, gene expression programs are usually switched to hypoxic response and neoangiogenesis, which ultimately lead to tumor egress and metastasis. Accordingly, gene sets involved in extracellular matrix remodeling, MAPK signaling, and response to hypoxia were up-regulated in M-EDCL (Figure 4D; Supplementary Table 4), consistent with their association to metastasis in other cancers (8, 9).
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Table 6A. PAG Delta Correlation Gene Networks Network 2 Network 3
Table 6A. PAG Delta Correlation Gene Networks Focus ID Molecules in Network Score Molecules Top Functions ANXA6, Ap1, B4GALT1, CALM3, Calmodulin, Caspase, CDH22, CNR1, DAPK1, DLEU2, E2f, EGF, F Actin, 1 FCGR2A, GAD1, H19, HBA2, Histone h3, Hsp70, Insulin, Lipid Metabolism, Small Jnk, NFkB, Ntrk1 dimer, PI3K, Pkc(s), Pld, PLIN, PRKCB1, Molecule Biochemistry, Skeletal Ras, RPA2, RUNX1, SHC1, TTK, TXN, UBE2C 44 19 and Muscular Disorders AKAP12, ASXL1, CCL27, CKS2 (includes EG:1164), CTCF, DAD1, DUSP5, EEF1G, ELK3, ETV1, HIPK1, 2 HIPK3, HOXB5, ITGB6, KIF23, KRT15, LDB1, NCAN, PCK2, PLIN, PPARG, PRC1, RACGAP1, retinoic acid, RNF111, SKIL, SMURF2, SOD3, TGFB1, Timp, TNF, TP53, Cancer, Cell Cycle, UBE2C, UBE2D3, VEGFA 17 9 Hematological Disease AKT3, amino acids, beta-estradiol, CBLC, CHGB, CKS1B, COL6A2, COL6A3, EIF4E, glutamic acid, GP5, hydrogen peroxide, IL4, IL6, LGR4, LSM2, LSM4, LSM5, LSM6, 3 LSM7, MTMR7, MX2, NPY1R, PIM2, PLG, PPP5C, Gene Expression, Cell Death, PRDX5, RLBP1, SGK3, SLC17A, SLC17A7, SV2B, SYT11, Nervous System Development Timp, TXNIP 17 9 and Function PAG Delta Corr Networks Network 1 Network 2 Network 3 Table 6B. PAG Saline Correlation Gene Networks Focus ID Molecules in Network Score Molecules Top Functions Akt, ARHGEF4, B4GALT1, Calpain, CASP3, E2f, H19, HMOX1, Hsp27, Hsp70, Insulin, Jnk, LDL, MAP2, Mapk, Cell Morphology, Nervous 1 MAPT, MCM6, NFkB, NFKB2, P38 MAPK, Pdgf, Pdgf Ab, System Development and PDGF BB, PI3K, Pkc(s), PP2A, PPP2R2C, PRDX2, PTN, Function, Cardiovascular Ras, RPS14, SERPINF1, Tgf beta, TMOD2,
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Autocrine IFN Signaling Inducing Profibrotic Fibroblast Responses by a Synthetic TLR3 Ligand Mitigates
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