IPO5 Antibody

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Product Datasheet IPO5 Antibody Catalog No: #32535 Orders: [email protected] Description Support: [email protected] Product Name IPO5 Antibody Host Species Rabbit Clonality Polyclonal Purification Antibodies were purified by affinity purification using immunogen. Applications WB,IF Species Reactivity Human,Mouse Specificity The antibody detects endogenous level of total IPO5 protein. Immunogen Type Recombinant Protein Immunogen Description Recombinant protein of human IPO5. Target Name IPO5 Other Names IMB3; Pse1; imp5; KPNB3; RANBP5 Accession No. Swiss-Prot:O00410NCBI Gene ID:3843 SDS-PAGE MW 123KD Concentration 1.0mg/ml Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Store at -20°C Application Details WB 1:500 - 1:2000IF 1:50 - 1:200 Images Immunofluorescence analysis of U2OS cells using IPO5 . Blue: DAPI for nuclear staining. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Western blot analysis of extracts of various cell lines, using IPO5 at 1:1000 dilution. Background Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2.

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KETCH1 Imports HYL1 to Nucleus for Mirna Biogenesis in Arabidopsis
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8484–8498 Nucleic Acids Research, 2012, Vol. 40, No. 17 Published online 22 June 2012 doi:10.1093/nar/gks598 NMDAR signaling facilitates the IPO5-mediated nuclear import of CPEB3 Hsu-Wen Chao1, Yen-Ting Lai1,2, Yi-Ling Lu1, Chi-long Lin3, Wei Mai3 and Yi-Shuian Huang1,2,* 1Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan, 2Graduate Institute of Microbiology, National Taiwan University, Taipei 10051, Taiwan and 3National RNAi Core Facility, Academia Sinica, Taipei 11529, Taiwan Downloaded from https://academic.oup.com/nar/article/40/17/8484/2411581 by guest on 01 October 2021 Received May 1, 2012; Revised May 23, 2012; Accepted May 28, 2012 ABSTRACT controlling gene expression, scaffold molecules, and the proteolyzed intracellular domains of synaptic receptors Cytoplasmic polyadenylation element-binding (4,5). Transcription factors, such as cyclic AMP respon- protein (CPEB)3 is a nucleocytoplasm-shuttling sive element-binding protein (CREB)2, and translation RNA-binding protein and predominantly resides in regulators, such as cytoplasmic polyadenylation element- the cytoplasm where it represses target RNA trans- binding protein (CPEB)3 and CPEB4, move to the nucleus lation. When translocated into the nucleus, CPEB3 after activation of the N-methyl-D-aspartate receptor binds to Stat5b and downregulates Stat5b-depen- (NMDAR) (6–9), suggesting that the nucleocytoplasmic dent transcription. In neurons, the activation of partition of these proteins by speciﬁc neuronal signaling N-methyl-D-aspartate receptors (NMDARs) accumu- determines their role in gene expression. lates CPEB3 in the nucleus and redistributes CPEB3 Synapse-to-nucleus communication (i.e. retrograde in the nucleocytoplasmic compartments to control trafﬁcking of protein molecules from synaptic areas to the nucleus) plays an important role in synaptic plasti- gene expression.
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