Product Datasheet

IPO5 Antibody

Catalog No: #32535

Orders: [email protected] Description Support: [email protected]

Product Name IPO5 Antibody

Host Species Rabbit

Clonality Polyclonal

Purification Antibodies were purified by affinity purification using immunogen.

Applications WB,IF

Species Reactivity Human,Mouse

Specificity The antibody detects endogenous level of total IPO5 .

Immunogen Type Recombinant Protein

Immunogen Description Recombinant protein of human IPO5.

Target Name IPO5

Other Names IMB3; Pse1; imp5; KPNB3; RANBP5

Accession No. Swiss-Prot:O00410NCBI ID:3843

SDS-PAGE MW 123KD

Concentration 1.0mg/ml

Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02%

sodium azide and 50% glycerol.

Storage Store at -20°C

Application Details

WB 1:500 - 1:2000IF 1:50 - 1:200

Images

Immunofluorescence analysis of U2OS cells using IPO5 . Blue: DAPI for nuclear staining.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Western blot analysis of extracts of various cell lines, using IPO5 at 1:1000 dilution.

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein , the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2