THE JOHN CURTIN SCHOOL OF MEDICAL RESEARCH Annual Review 2013
ANU College of Medicine, Biology & Environment
CONTENTS
Annual Review 2013 The John Curtin School of Medical Research
From the Director 3 Staff and student lists 87
Department of Genome Biology 88 Organisation Chart 4 Department of Immunology 90 Committees 5 Eccles Institute of Neuroscience 92 Department of Molecular Bioscience 94 Community 7 Department of Translational Medicine 96 Department of Pathogens and Immunity 96 Centenary of Canberra 2013 Australian Phenomics Facility 97 14th Frank and Bobbie Fenner Conference 8 School Administration 98 The John Curtin Medical Research Foundation Dinner 9 School Services 98 David Curtis Reading Room 10 The ACRF Biomolecular Resource Facility Open Day 2013 11 and Genome Discovery Unit 99 Community Activities 12 Students 100
Research in Review 2013 15 Publications, presentations and Eccles Institute of Neuroscience 16 community outreach 103
Department of Genome Biology 30 Publications 104 Department of Immunology 38 Staff Presentations 110 Department of Pathogens and Immunity 48 Student Presentations 117 Department of Molecular Bioscience 52 Community Outreach 121 Department of Translational Medicine 58 Australian Phenomics Facility 60 Support to JCSMR 131
The Imaging and Cytometry Facility 62 Grants 132 The ACRF Biomolecular Resource Facility Financial Overview 136 and Genome Discovery Unit 63 Donors 137 Staff and student achievements 65 Sponsors 138
Graduates 66 Prizes and Awards 67
Visitors and collaborations 71
Visitors 72 Collaborations 74
Annual Review 2013 1 2 The John Curtin School of Medical Research FROM THE DIRECTOR
Following the opening of Stage 3 of our new building in 2012, JCSMR staff and students have enjoyed the opportunities provided by the new spaces, including the laboratories, and meeting rooms such as the Science Forum. In order to ensure that we do not lose sight of our history and past achievements, we have decided to name some of our rooms in honour of Emeritus staff members. We began this tradition in 2009, naming the Videoconferencing Room in memory of Professor Gordon Ada, a leading immunologist, and a laboratory in honour of Professor Frank Gibson, a pre-eminent biochemist. The first room to be officially named in Stage 3 is the David Curtis Reading Room, named in honour of Professor David Curtis. Professor Curtis was Director of JCSMR from 1989 until his retirement in 1992. Professor Curtis has generously donated his books and manuscripts to be housed in the Reading Room, and the inclusion of current journal issues and comfortable Fred Ward armchairs provides staff and students with a very pleasant quiet and sunny environment in which to catch up on their reading. Toward the end of 2013, a full Review of Medical and Health Canberra celebrated its Centenary during 2013, and throughout Sciences was undertaken at ANU. JCSMR will seek to the year The John Curtin School of Medical Research implement a number of the recommendations of the review over participated in those celebrations with our community. We the coming year, including restructuring of our Departments, and hosted conferences, invited the public to our School for lectures increasing support to our early and mid-career researchers. I and open days, heard presentations by Nobel Laureates, look forward to bringing you updates on our progress. congratulated the John Curtin Medical Research Foundation on its first year of operation, and continued to undertake I would like to mention a few very important Honours and ground breaking medical research. As an ongoing legacy Awards achieved by JCSMR staff and students in 2013. of the Centenary, the ACT Government partnered with The Congratulations to Professor Chris Goodnow, Head of the Australian National University to establish a Centenary Chair in Immunology Department, who was elected a Member of the Cancer Research at JCSMR, to strengthen and develop our US National Academy of Sciences in 2013, in recognition of cancer research program. We expect to appoint the successful distinguished and continuing achievements in original research. candidate in 2014. The Future Justice Prize, which recognises individuals and organisations that are making a significant contribution to In 2013, JCSMR Director Professor Julio Licinio left the School the advancement of the present generation whilst securing to join the South Australian Health and Medical Research and strengthening the life chances of future generations, was Institute (SAHMRI) as Deputy Director for Translational Medicine, awarded to Professor Jill Gready. Dr Anselm Enders, Leader of and Head of the Mind and Brain Theme. Staff and students the Ramaciotti Immunization Laboratory received the inaugural thanked him for his efforts at JCSMR at a farewell in April, and Gordon Ada Young Investigator Award, and presented the 2013 he has our continued thanks for his efforts in establishing both Gordon Ada Oration. The Finkel Prize, which recognizes research Translational Medicine at ANU, and The John Curtin Medical which leads to better health outcomes in developing countries, Research Foundation (JCMRF). This Foundation, established was awarded to Dr Dan Wijesundara. Congratulations to all in 2012, celebrated its First Anniversary in March with a Gala these scientists on the important work they have undertaken to Dinner at Old Parliament House. Nobel Laureate Professor Peter receive these awards. Doherty, and ACT Chief Minister Ms Katy Gallagher spoke to guests at the dinner about the importance of medical research in I hope that you enjoy reading this Review, which provides a day-to-day life, and ways in which the community could provide snapshot of the year 2013. For further details of our current their support. For more information about the work of JCMRF research projects, and the activities of our staff and students please see: jcmrf.anu.edu.au please visit: jcsmr.anu.edu.au JCSMR worked closely with the ACT Government Centenary Professor Christopher Parish Committee to celebrate World Day of Immunology in April, by bringing our two living Nobel Laureates back to Canberra. Both Director, The John Curtin School of Medical Research Professor Peter Doherty and Professor Rolf Zinkernagel participated August 2014 in the 14th Frank and Bobbie Fenner Conference, and each subsequently presented a public lecture in the series: Big Ideas, Big Talk. ANU students and researchers, as well as members of our local community, were delighted to have the opportunity to hear these two eminent scientists speak, and to meet with them individually over refreshments. Their presence certainly added to our celebration of 100 years of Science in Canberra.
Annual Review 2013 3 ORGANISATION CHART
ANU College of Medicine, Biology and Environment
The John Curtin School of Medical Research 2013
Director Professor Julio Licinio (until April) Professor Chris Parish (from April) Deputy Director Professor Phil Board DEPARTMENTS
Genome Immunology The Eccles Pathogens Molecular Translational Biology Institute of & Immunity Bioscience Medicine Neuroscience
Professor Professor Professor Professor Professor Professor David Chris Greg Carola Angela Dulhunty Julio Licinio Tremethick Goodnow Stuart Vinuesa (until April) Dr Claudio Mastronardi (from April)
ADMINISTRATION
School Manager Outreach & Education Communications
Mr Gerry Lillicrap Dr Madeleine Nicol Dr Anna Cowan Ms Laura Vitler
SERVICES AND SUPPORT
ACRF Australian Operations Microscopy & Technical Safety & Biomolecular Phenomics Cytometry Services Training Resource Facility Resource Facility Facility
Ms Stephanie Dr Steve Mr Denis Ms Cathy Mr Denis Ms Sonya Palmer Winslade Coombes Gillespie Coombes Glasson (until July) Dr Mark Hayes (acting from July)
4 The John Curtin School of Medical Research COMMITTEES
Executive Committee Operations Committee New Revenue Streams Professor Philip Board Dr Jennifer Clancy Committee (until April) Dr Anna Cowan Mr Denis Coombes Dr Vicki Athanasopoulos Professor Angela Dulhunty Professor Angela Dulhunty (Chair) Dr Ed Bertram Professor Chris Goodnow Dr Suzanne Fowler Professor Philip Board Professor Julio Licinio (Co-Chair) (until April) Ms Cathy Gillespie Ms Anneloes De Graeff Mr Gerard Lillicrap Ms Sonya Glasson (until June) Professor Angela Dulhunty Dr Claudio Mastronardi (from July) Dr Mark Hayes (from July) Professor Simon Easteal Dr Madeleine Nicol (from October) Professor Caryl Hill Dr Julia Ellyard Professor Chris Parish (Co-Chair) Mr Gerard Lillicrap Professor Julio Licinio Professor Greg Stuart Ms Stephanie Palmer Mr Gerard Lillicrap Professor David Tremethick Dr Charani Ranasinghe Professor Ted Maddess Professor Carola Vinuesa Ms Michelle Townsend Dr Madeleine Nicol Mr Dennis Vukoja Professor Chris Parish External Relations Mrs Esmee Weil Mr Geoff Sjollema Committee Professor Greg Stuart Safety Committee Dr Steve Winslade (Chair) Professor Philip Board (Chair) Dr Anna Cowan Professor Philip Board (Chair) (until August) Dr Anselm Enders Ms Megan Clydesdale Workshop Committee Ms Anneloes De Graeff (until September) Mr Mick Devoy Professor Angela Dulhunty (Chair) Professor Julio Licinio (until April) Dr Renae Domaschenz Professor Caryl Hill Mr Gerard Lillicrap Dr Julia Ellyard Mr Denis Coombes Dr Riccardo Natoli (from February) Dr Sonya Glasson (until June) Ms Michelle Townsend Dr Madeleine Nicol Dr Mark Hayes (from July) Mr Gerard Lillicrap Professor Chris Parish Mrs Angela Higgins Professor Thomas Preiss Mr Gerard Lillicrap Gender Equity Committee Dr Peter Milburn (from September) Group Leaders Forum Ms Suzy Pace Ms Jackie Stenhouse Associate Professor Ehsan Arabzadeh Professor Carola Vinuesa (Chair) Dr Krisztina Valter (Chair) (from September) Associate Professor Ian Cockburn All Group Leaders and Laboratory Leaders Dr Lucy Coupland belong to the Group Leaders Forum, Dr Julia Ellyard (Chair) which meets monthly to discuss matters of Animal Users Committee Dr Rebecca Haddock importance relating to JCSMR Associate Professor Ruth Arkell (RSB) Associate Professor Brett Lidbury Dr Anneke Blackburn Dr Lisa Miosge Education Committee Mr Anthony Barker (ex officio) (from October) Dr Madeleine Nicol Professor Philip Board Associate Professor John Bekkers Dr Ian Parish Ms Katrina Bowditch (ex officio) Dr Anneke Blackburn Dr Rebecca Sweet Ms Megan Clydesdale (ex officio) (until Dr Anna Cowan (Chair) Professor Carola Vinuesa September) Professor Simon Easteal Dr Lucy Coupland Miss Preethi Eldi Professor Angela Dulhunty Professor Chris Parish Dr Suzanne Fowler (ex officio) Mr Roy Ramiscal Associate Professor Guna Karupiah Professor Ma-Li Wong (until April) Associate Professor Brett Lidbury Professor Klaus Matthaei Dr Riccardo Natoli Dr Ian Parish Dr Charani Ranasinghe Dr Charmaine Simeonovic Associate Professor Christian Stricker (Chair) Dr Narci Teoh (Canberra Hospital)
Annual Review 2013 5 6 The John Curtin School of Medical Research Community
Centenary of Canberra 2013 14th Frank and Bobbie Fenner Conference The John Curtin Medical Research Foundation 1st Anniversary Dinner The David Curtis Reading Room Our Community Open Day
Annual Review 2013 7 CENTENARY CELEBRATIONS 2013 14TH FRANK AND BOBBIE FENNER CONFERENCE
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In conjunction with celebrations of the Centenary of Canberra 1. Nobel Laureate Professor Rolf Zinkernagel presents his Keynote address in 2013, and timed to coincide with World Day of Immunology, 2. Professor Chris Goodnow and Professor Chris Parish at the Fenner Conference 3. Professor Rolf Zinkernagel and Dr Peter Jeffrey The John Curtin School of Medical Research hosted the 4. Dr Jane Dixon, Nobel Laureate Professor Peter Doherty, Dr Misty Jenkins 14th Frank and Bobbie Fenner Conference on Immunological and Dr Geeta Chaudhri 5. Professor Ian Fraser, Dr Gabrielle Belz and Professor Peter Doherty Research in April. Support from the Centenary committee allowed 6. Invited Speakers at The 14th Frank and Bobbie Fenner Conference us to bring two of our world-renowned scientists, Nobel Laureates Professor Peter Doherty and Professor Rolf Zinkernagel back to Canberra for the event. Both presented a keynote seminar at the conference, and also gave a public lecture to which the whole Canberra community was invited, in the series: Big Ideas, Big Talk. Both the conference and the public events were exceptionally well attended, and JCSMR received congratulatory messages to say how wonderful it was that in Canberra, we have the opportunity to hear such eminent speakers.
8 The John Curtin School of Medical Research THE JOHN CURTIN MEDICAL RESEARCH FOUNDATION DINNER
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The John Curtin Medical Research Foundation was 1. ANU Vice-Chancellor and President Professor Ian Young established in 2012 to raise the public profile of medical 2. ACT Chief Minister Ms Katy Gallagher 3. Nobel Laureate Professor Peter Doherty research at The Australian National University. 4. MC for the event Mr Hugh Riminton, Nobel Laureate and guest speaker Professor Peter Doherty and Foundation Chair Dr Cameron Webber Through the tireless efforts of the Board Members, much 5. Guests at the Foundation Dinner needed funds are being raised to support young researchers 6. The Members Dining Room, Old Parliament House through the early stages of their academic career, and to purchase important items of equipment required to ensure we continue our cutting edge research projects. The Foundation Board held a Gala Dinner at Old Parliament House in March 2013 to celebrate their 1st Anniversary. For more information about the Foundation visit: jcmrf.anu.edu.au
Annual Review 2013 9 THE DAVID CURTIS READING ROOM
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On Friday 12 July 2013, family, friends and colleagues gathered 1. Professor Chris Parish with the Curtis Family 2. Professor Chris Parish and Emeritus Professor David Curtis at the dedication at a small ceremony to dedicate the David Curtis Reading Room of the Reading Room on level 3 of the JCSMR Building. 3. The commemorative plaque 4. Mrs Joanna Webber, Professor Curtis and Dr Cameron Webber The room is named in honour of Emeritus Professor 5. Emeritus Professor William Levick and Emeritus Professor David Curtis in the newly David R Curtis AC MBBS PhD FAAS, Head of the JCSMR dedicated Reading Room Department of Pharmacology (1973 – 1989) 6. Ms Belinda Curtis and Professor David Curtis and Director of the School (1989-1992). Our thanks to Professor Curtis for his very kind gift to the School of his many books and manuscripts, which are now housed in this room for the benefit of all our staff and students.
10 The John Curtin School of Medical Research OPEN DAY 2013
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We invite our local community in to see through The 1. Tiffany Cripps describing the work of the Biomolecular Resource Facility to Open Day visitors John Curtin School of Medical Research on our Annual Open 2. Dr Jason Potas and a young visitor to JCSMR Open Day Days. We warmly invite you to attend our next Open Day, speak 3. Hands on science experiments on Open Day with a scientist, take a Discovery Tour of our building, and hear 4. Dr Angelo Theodoratos explaining his research 5. Tatiana Vassilieva speaking about research on crop improvements about the important research we undertake in fields including to feed the world as the population increases cancer, diabetes, vision, and autoimmune disease. 6. Open Days offer the opportunity for a closer look at JCSMR research! For information about our next Open Day, please visit jcsmr.anu.edu.au
Annual Review 2013 11 COMMUNITY ACTIVITIES
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1. The Sarah Grace Sarcoma Foundation presenting a cheque to Professor Chris Parish and Dr Lucy Coupland to support a PhD Scholarship in Sarcoma research at JCSMR 2. His Excellency Mr Raul Gangotena, Mr Gerry Lillicrap, Professor Chris Parish and Mr Jeremy Hansen at a Christmas morning tea to celebrate a successful year at JCSMR 3. Mr John Milne and Professor Chris Goodnow with Dr John Altin who was awarded the Dewar-Milne Prize in Immunology 4. Mrs Nancy Ganter, Ms Janet Henry and Mrs Helen Benneworth at The JCSMR Director’s Morning Tea in December 5. Ms Diana Lipscombe, Dr Rose Mason, Ms Belinda Curtis and Ms Marilyn Fenner at JCSMR to celebrate the 50th Anniversary of the award of the Nobel Prize to Sir John Eccles in 1963 6. Professor John Bekkers' laboratory hosted National Youth Science Forum visitors in January 7. National Youth Science Forum students visit the School 8. Professor Peter Doherty, Mr Neil Ada and Ms Louise Spencer congratulate Dr Anselm Enders, recipient of the inaugural Gordon Ada Early Career Researcher Award
Annual Review 2013 13 14 The John Curtin School of Medical Research Research in Review 2013
Eccles Institute of Neuroscience Department of Genome Biology Department of Immunology Department of Molecular Bioscience Department of Pathogens and Immunity Department of Translational Medicine The Australian Phenomics Facility The Imaging and Cytometry Facility Australian Cancer Research Foundation Biomolecular Resource Facility
Annual Review 2013 15 Eccles Institute of Neuroscience Head of Department Professor Greg Stuart
Group Leader Blood Vessel Group Professor Caryl Hill Neuroimmunology & Cardiovascular Dysfunction Laboratory Dr Rebecca Haddock Cerebral Cortex Group Associate Professor John Bekkers Diagnostics for Eye Diseases Group Professor Ted Maddess Neural Coding Group Associate Professor Ehsan Arabzadeh Neuronal Network Group Associate Professor Christian Stricker Neurophotonics Laboratory Dr Vincent Daria Neuronal Signalling Group Professor Greg Stuart Brain Development Laboratory Dr Zan-Min Song Neuronal Integration Laboratory Professor Greg Stuart Retinal Cell Damage and Repair Group Dr Krisztina Valter-Kocsi Retinal Development and Aging Group Professor Jan Provis Neural Control Systems Laboratory Dr Jason Potas Synapse and Hearing Group Professor Bruce Walmsley Visual Neuroscience Group Professor Trevor Lamb
16 The John Curtin School of Medical Research NEW TREATMENTS FOR HIGH BLOOD PRESSURE
Blood vessels constrict due to the activity In the muscle cells, we have found that Eccles Institute of muscle cells in their walls and relax the oxidative stress which accompanies due to factors released by the layer of cardiovascular disease is responsible for of Neuroscience cells which line them; the endothelium. changes in the mechanisms by which Our studies have shown that small small resistance vessels constrict. In our arteries are subject to multiple diverse animal models of cardiovascular disease, Blood Vessel Group stimuli that can cause relaxation or we find that calcium influx, which is constriction. Importantly, blood vessels essential for vascular constriction, now Professor Caryl E Hill need to integrate these opposing stimuli involves an additional ion channel in the Hypertension is a major risk factor for to produce a coordinated response and cell membrane, which was not previously cardiovascular disease, which itself is maintain adequate tissue perfusion. This considered to play this role and we have the leading cause of premature death coordination results from the coupling identified the enzyme which causes worldwide. However, some 30% of of cell activity within the vascular wall by these changes. Our current studies are hypertensive patients exhibit a therapy channels called gap junctions. aimed at testing whether hypertension resistant form which does not respond to can be ameliorated by pharmacological current clinical treatments. We have tested the importance of the endothelium and gap junctions by antagonism of these targets. The regulation of blood pressure depends developing genetically modified mice Our studies are providing and testing on the ability of the heart to pump blood with endothelial-selective defects in their new targets which impact on the and on the function of the smallest blood gap junctions. We have found that these peripheral vasculature and its role in the vessels within tissues and organs around mice develop arterial stiffening and mild regulation of blood pressure. our body. These small vessels provide hypertension, characteristics which occur the greatest resistance to blood flow and jcsmr.anu.edu.au/research/eccles- during ageing and cardiovascular disease. institute-neuroscience/blood-vessel efficient tissue oxygenation. We propose Our results demonstrate that the proteins that many adverse cardiovascular which are important for coordination of the events result from alterations to the endothelium are crucial to the maintenance function of these microvessels and that of peripheral vascular function. studying these changes will identify novel therapeutic targets for the treatment of therapy resistant hypertension.
Annual Review 2013 17 NEUROIMMUNOLOGY & CARDIOVASCULAR DYSFUNCTION
is considered neurogenic. Work in our Oxidative stress and the Eccles Institute laboratory has revealed that increases sympathetic nervous system in blood pressure in obesity occur as a Elevation in the production of reactive of Neuroscience consequence of an increase in the growth oxygen species (ROS) in the vascular of sympathetic nerves that cover the wall is a key feature of cardiovascular surface of blood vessels important for diseases, including hypertension and Neuroimmunology & controlling blood pressure. This increased obesity. Excessive ROS production in Cardiovascular Dysfunction innervation leads to increased nerve- obesity is known to lead to increased mediated vasoconstriction, increased Laboratory oxidative stress and inflammation; as well peripheral resistance and increased as to dysregulation of the sympathetic blood pressure. The focus of our group Dr Rebecca Haddock nervous system and control of blood is therefore to understand the pathways Cardiovascular disease is the leading pressure. We have been investigating the that control these processes with a view cause of death in Australia and high blood link between oxidative stress and changes to improving pharmaceutical treatment pressure, or hypertension, is the single in the vascular sympathetic nerve of hypertension in the overweight and biggest risk factor for developing this network that occur in obesity-related obese. disease. The causes of hypertension are hypertension. This research increases our both biomedical and lifestyle oriented; Interaction between the immune understanding of the interaction between with the probability of developing the immune and sympathetic nervous and sympathetic nervous systems hypertension being significantly increased systems in this disease and provides in individuals who are overweight or Increasing evidence suggests that new therapeutic targets for reducing the obese. Today over 60% of the Australian cells of the immune and sympathetic more harmful effects of obesity on the adult population is considered overweight. nervous systems ’talk’ with each other cardiovascular system. in order to maintain proper blood Clinically, successful treatment of pressure regulation. Given that obesity jcsmr.anu.edu.au/research/ obesity-related hypertension is often is recognized as a lowgrade, chronic eccles-institute-neuroscience/ problematical, with many overweight inflammatory disease, this interaction neuroimmunology-cardiovascular- patients being resistant to first-line is likely disturbed under hypertensive dysfunction anti-hypertensive treatments. This is conditions that occur in the overweight further complicated by the fact that and obese. We have shown that some treatments are associated with increased infiltration of nerve growth factor unwanted side effects on the metabolic (NGF)- producing immune cells into the abnormalities that occur in the overweight vascular wall is not only crucial, but also a and the obese. prerequisite, to pathological sympathetic The incidence of hypertension and nerve growth and the development of obesity is linked to increased activity hypertension in the obese. of the sympathetic nervous system. Therefore obesity-related hypertension
18 The John Curtin School of Medical Research USING SMELL AS A GATEWAY TO BRAIN CIRCUITS
Eccles Institute of Neuroscience
Cerebral Cortex Group
Associate Professor John Bekkers Brain diseases come in many forms, but roughly speaking there are two broad types. The first type are diseases that cause the death or degeneration of nerve cells (neurons) in the brain. These are called neurodegenerative By understanding how this particular In another experiment we have been disorders and include Alzheimer’s brain circuit makes sense of the world using a laser microscope to image Disease and Parkinson’s Disease, of odours, we hope to uncover general individual neurons in the olfactory cortex, which mainly affect older people. The principles about the operation of brain both under normal conditions and when second type are diseases that occur circuits that will help us to understand the cortex is experiencing an epileptic when neurons are healthy but are wired what goes wrong in circuit disorders. seizure. The figure shows an example up incorrectly to other neurons. These Our strategy has been to take a bottom- from this experiment. The green ovals are are called circuit disorders and include dye-filled neurons, which change their schizophrenia, autism and epilepsy, up approach, first by working out the circuit diagram for the olfactory cortex brightness when they are active during which occur in young as well as older a seizure (coloured traces on right). This people. Unfortunately, our understanding of mice by studying small pieces of mouse brain in a dish, and second by method allows us to study how seizures of circuit disorders lags well behind that spread through this particular brain circuit. of neurodegenerative disorders, in part measuring what this circuit actually does because our understanding of brain in the whole animal. During the past Although the brain is an exceptionally circuits is so primitive. In my laboratory we year we have made excellent progress complex organ, we are optimistic that our are trying to fill this gap in our knowledge towards achieving our aims. For example, work will reveal much about brain circuits of circuits by studying one of the simplest a PhD student in the group has found and why they go wrong in diseases. brain regions, the primary olfactory cortex. that one type of neuron in the olfactory jcsmr.anu.edu.au/research/eccles- cortex responds to fewer odours than institute-neuroscience/cerebral-cortex The olfactory cortex is located at the base other types of neurons, supporting a of the front of the brain, just behind the hypothesis we developed from our earlier nose. Its job is to enable us to recognise experiments in the dish. By analysing the and remember odours by building up an pattern of responses we can work out ‘odour image’ when we sniff the bouquet how different types of neurons process of chemicals that comprise an aroma. odour information.
Annual Review 2013 19 DISEASES OF THE VISUAL SYSTEM
involves developing a new automated Eccles Institute perimeter, which displays multiple stimuli to both eyes at the same time, and also of Neuroscience objectively measures the responses of the two eyes at the same time. No button presses are needed because the Diagnostics for Eye responses of the eyes are determined by Diseases Group automatically tracking the diameter of the test subject’s pupils. That also makes the Professor Ted Maddess new test non-contact. Overall the new Our lab attempts to understand vision test is thus potentially faster and more and also to find ways to quantify accurate. After 8 years work we now have functional impairment of our visual a well-developed prototype instrument. system. That work is aided by our new We are currently seeking investment set of labs that opened last year at partners to bring the instrument to JCSMR. The new labs are collectively market. In 2013 we completed projects known as the JCSMR Clinical Suites. that used the prototype to investigate: Much of our research is about trying diabetic retinopathy, glaucoma, epilepsy, to develop better ways to quantify macular degeneration, migraine, and the patchy blindness that occurs in a multiple sclerosis. The basic findings were number of eye diseases and neurological that our standard tests did not cause problems. The distribution and extent of seizures or migraine (but could detect if a such patchy blindness is conventionally person had had a recent migraine), and mapped by devices called perimeters. was very good at accurately diagnosing Our past research led to the development early-stage retina disease. In other studies of the FDT/Matrix perimeters sold by Carl we have investigated limitations of some Zeiss, which have been on the market standard ophthalmic test instruments. We for several years, thus earning the ANU also completed studies on the basis of some income. Perimeters conventionally normal visual function. involve presenting light stimuli at a set of jcsmr.anu.edu.au/research/eccles- standard test locations in front of subject, institute-neuroscience/diagnostics-eye- i.e. at locations across their field of view. diseases Each time a stimulus is presented the subject presses a button to report that they saw it. That process is slow and prone to errors. Much of our current work
20 The John Curtin School of Medical Research DECIPHERING THE LANGUAGE OF BRAIN CELLS
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Associate Professor Ehsan Arabzadeh An important challenge to Neuroscience is to understand how the brain generates a reliable representation of the world around us. Our group focuses on an “expert” system, the rodent whiskers, which are used to sense the world around the rat. We investigate how the elegant from cortex and deep brain structures in The system is well suited to examining cortical circuitry underpins efficient anaesthetised as well as awake behaving neural coding issues because of its processing of the sensory signals that rodents, and apply methods such as functional efficiency and its elegant can guide the animal’s decisions. Over information theory to quantify the way by structural organisation. The whisker area the last decade, new electrophysiological which neurons represent sensory stimuli of somatosensory cortex (known as barrel and imaging methods have emerged for or control the animal's behaviour. cortex) is arranged as a topographic observation of neuronal activity. These map of the whiskers. This means that methods allow us to relate a detailed and The rodent whisker system sensory signals arising in one whisker are quantitative characterisation of sensation channelled through a restricted population and behaviour to the underlying cellular The rodent whisker-barrel pathway is of cells and can be characterised by and molecular mechanisms at work our model system of choice. It is also recording electrodes or through optical in the brain. The lab has a broad interest the rat's sensory system of choice for imaging. in systems neuroscience spanning areas exploring the environment and collecting jcsmr.anu.edu.au/research/eccles- such as sensory coding and adaptation. information about the location, shape, institute-neuroscience/neural-coding We characterise the activity of brain cells size and texture of objects around it.
Annual Review 2013 21 COMMUNICATION BETWEEN NERVE CELLS
Noradrenaline and serotonin are two Dissecting the molecular mechanism, Eccles Institute neuromodulators in the brain, which we have found that the depression of are released diffusely by cells located in action potential mediated release is of Neuroscience specific nuclei. Both have the ability to caused by a signalling step immediately signal via G protein-coupled receptors, after G protein activation, but before which can lead to IP3 production. Drugs phospholipase C activation. This Neural Network Group that mimic their action are known for suggests that the depression by their abuse potential (amphetamines, noradrenaline and serotonin is most likely Associate Professor LSD). In addition, many antipsychotic caused by Gβγ directly affecting the Christian Stricker drugs have a strong anti-adrenergic/ release machinery. Release of neurotransmitter in the serotonergic effect. Yet the way by which The fact that both neuromodulators form of vesicles is the means by which both affect communication between cause increased spontaneous, but nerve cells communicate with each neurons is poorly understood. The aim of depressed action potential mediated other. There are two modes by which our research is to unravel the molecular release, points to the fact that either transmitter is released: spontaneous mechanisms involved in modulation of there are two distinct mechanisms of and action potential mediated release. neuronal networks. vesicle release or that there are two The former is due to random release Surprisingly, we have found that both populations of vesicles that can be of vesicles of transmitter, the latter noradrenaline and serotonin increase released in nerve terminals. These due to calcium-mediated release of spontaneous transmitter release via findings shed new light on the molecular synchronised vesicles. In recent years, their action to increase IP3 production. mechanisms underlying transmitter we have found evidence that release However, action potential evoked release. of calcium from calcium stores in transmitter release was depressed. nerve endings increases the release jcsmr.anu.edu.au/research/eccles- This reduction is caused downstream of transmitter. In particular, signalling institute-neuroscience/neuronal-network of the same receptor activation. In fact, via receptors on the nerve endings spontaneous release remains elevated that produce the molecule IP3 causes while action potential mediated release increased spontaneous release. Using is depressed. This depression happens calcium imaging directly from individual despite increased calcium release from nerve terminals, we now have direct stores. evidence that spontaneous release of calcium occurs in these nerve terminals.
22 The John Curtin School of Medical Research OPTICAL TECHNIQUES TO ANALYSE THE BRAIN
focus to monitor changes in fluorescence, Eccles Institute enabling optical recording of neuronal of Neuroscience activity. How a neuron processes all these input signals, ultimately leading to an output Neurophotonics Laboratory (or action potential), is an important problem in neuroscience that is not yet Dr Vincent Daria fully understood. Such venture could The neurophotonics group is involved in provide us with a better understanding of a multi-disciplinary collaborative venture how these neurons process information between physicists and neuroscientists thereby extending our capacity to to make use of novel optical techniques understand neuronal dysfunction, e.g. to analyse the brain. In this collaboration, neuropsychiatric and neurodegenerative we develop a two-photon microscope, diseases. The dynamic properties of using which incorporates a 3D holographic light induced generation and recording of laser projector. The microscope allows neuronal signals have clear advantages us to render the 3D morphology of a over conventional electrical stimulation neuron while the holographic projector using micropipettes. This will significantly produces multiple laser foci projected speed up the study of neuronal circuits, along the neuron’s dendritic tree. Each which brings us closer to understanding focus functions as a probe capable of how the brain works. manipulating and recording neuronal jcsmr.anu.edu.au/research/eccles- activity. To manipulate neuronal institute-neuroscience/neurophotonics activity, we introduce chemically caged neurotransmitters, which can be uncaged by light. Using the holographic laser projector, we can dynamically configure the light pattern to uncage the neurotransmitter and locally stimulate synapses at multiple sites along the dendritic tree of a neuron. This process emulates the many synapses neurons receive from neighbouring neurons. In addition, using calcium sensitive and voltage-sensitive dyes, we can use each
Annual Review 2013 23 UNDERSTANDING THE BRAIN NEURON BY NEURON
different cell types, and can be regulated Signalling in dendritic Eccles Institute by external pH. We hypothesis this may help to maintain inhibition at a constant spines of Neuroscience level during hypoxia. This research has Input onto many neurons in the brain relevance to stroke. occurs onto specialized projections on Neuronal Signalling Group dendrites called dendritic spines. Calcium Coding of visual signaling in dendritic spines is known to be Professor Greg Stuart critical of induction of synaptic plasticity, information which is thought to underlie memory The Neuronal Signalling Laboratory formation in the brain. We have recently within the Eccles Institute conducts basic Visual information received by our eyes shown that calcium signaling in dendritic research on how nerve cells in the brain is combined in the brain to form a single spines during action potentials is regulated process information. This work involves view of the outside world. In order to by calcium-activated potassium channels recording activity from single nerve cells generate a single coherent map of localized within spines. This research has (technically called neurons) using both visual space, input from the two eyes is relevance to diseases associated with loss electrical and optical techniques. Over combined in the brain. This convergence of memory such as Alzheimer's disease. the past year we have been investigating occurs at the level of the cortex. How the following issues: the cortex integrates visual information jcsmr.anu.edu.au/research/eccles- from the two eyes is unclear. In this institute-neuroscience/neuronal- project we have been investigating how signalling Inhibition in the brain single neurons in primary visual cortex There are two main types of neurons process information from the two eyes. in the brain: Those that increase nerve This research has relevance to diseases impulse generation (excitatory cells) of the visual system as occurs in various and those that reduce nerve impulse forms of blindness. generation (inhibitory cells). These different cell types work in balance to regulate brain activity. The main chemical transmitter used by inhibitory neurons is the amino acid gamma aminobutyric acid, or GABA for short. In this project we have investigated the mechanism of action of a particular type of GABA receptor called the GABA_B receptor. In contrast to earlier work we find that the coupling of this receptor to the pathway that ultimately leads to inhibition is different in
24 The John Curtin School of Medical Research STUDIES ON HIRSCHSPRUNG’S DISEASE
There is a strain of rat with Hirschsprung’s Since this rat model has a mutation in Eccles Institute disease, which has a mutation in the the same gene as some patients with same gene (endothelin receptor B) and Hirschsprung’s disease, this study will of Neuroscience has the same phenotype as humans with shed light on brain abnormalities in Hirschsprung’s disease. Our recent study human Hirschsprung’s disease and will in the neonatal rats with Hirschsprung’s provide a scientific basis for future clinical Brain Development disease showed an increased cell death intervention. More generally in describing Laboratory and decreased nerve cell production the effects of a mutation in this gene in in several brain regions including the Hirschsprung’s rats, we are describing the Dr Zan-Min Song cerebellum and the hippocampus. effect of a gene on general neurological The large intestine is controlled by the The cerebellum is responsible for motor development, which may have effects nerve cells within the gut wall. Hence, coordination and the hippocampus is relevant to the general population in the lack of these neurons has serious involved in learning and memory. To health and ageing. consequences from bowel obstruction overcome the problem of premature jcsmr.anu.edu.au/research/eccles- to death. About 1/5000 infants are born death of mutated rats, we developed a institute-neuroscience/neuronal- with a blocked gut due to the lack of surgical procedure (colostomy) to make signalling/brain-development nerve cells in the wall of the large bowel, the gut to open on the abdominal wall a condition known as Hirschsprung’s in neonatal rats, which allows the rats disease. Although surgical removal of to survive into adulthood (6 weeks). affected gut is a life saving procedure, This operation makes our study of the some patients are left with other structural and molecular changes in neurological disorders, including seizures, brains and associated functional changes mental retardation and brain related in adult Hirschsprung’s rats possible for breathing problem or deafness. The the first time. structural and molecular changes in the We hypothesize that the brain changes brains of these patients are unknown that we observed in neonatal mutant rats due to the lack of brain tissues. will extend into adulthood and create functional deficits. We will analyse the brains of adult mutant rats in three major aspects: structural changes, molecular changes and functional changes.
Annual Review 2013 25 RETINAL DISEASES AND INJURY
The retina is the outpost of the brain, Eccles Institute containing mainly nervous tissue, but there is another important group of cells of Neuroscience also present in the tissue, glia. One such cell is the Muller cell, the principal support structures in the retina. As part Retinal Cell Damage and of their role, they respond to injury by Repair Group activating an immune response, produce neuroprotection and in the later stages Dr Krisztina Valter-Kocsi of the disease, by the formation of glial Our laboratory is looking at the processes scars to ‘heal’ the injured tissue. To that occur during retinal injury or disease, modulate these responses and minimise in order to understand how the retina may scar formation offers another important be healed. Ultimately, our goal is to identify therapeutic target. Our results so far and test therapies to provide benefit to have shown that treatment with 670nm sufferers of some retinal diseases. light reduces the Muller cell reaction and proliferation. Our laboratory provided scientific data to suggest that 670nm red light irradiation jcsmr.anu.edu.au/research/eccles- might be a useful treatment against the institute-neuroscience/retinal-cell- loss of photoreceptors, the principal vision damage-and-repair cells in the retina. We have shown the protective effect of 670nm light in retinal damage caused by bright light or high oxygen concentration. Our latest findings in models of retinopathy of prematurity (ROP) were the first to demonstrate its potential benefit in this condition. This therapy is non-invasive and may prove to be an inexpensive preventative treatment for ROP and chronic lung disease associated with prematurity. Encouraged by the success of these results, a Phase I clinical trial was initiated in the Canberra Hospital this year.
26 The John Curtin School of Medical Research MACULAR DEGENERATION
jcsmr.anu.edu.au/research/eccles- Eccles Institute institute-neuroscience/retinal- of Neuroscience development-and-aging
Retinal Development and Aging Group
Professor Jan Provis We are interested in development and aging of the retina, especially the macula. The macula is responsible for all of our ‘useful’ vision, mediated by very high densities of neurons and photoreceptors needed to process visual information with a high degree of accuracy. As we age, and depending on genetic predisposition and lifestyle choices (eg, smoking), this high level of metabolic activity often leads to localized oxidative stress and onset of low level / para-inflammatory processes. We have been using a bright light-induced rat model of retinal degeneration to better understand the progression of oxidative stress towards inflammation and degeneration. We find a range of genes expressed that is strikingly similar to those identified in human retinas affected by age-related macular degeneration, including chemokines that attract macrophages into the retina from the periphery. Developing a better understanding of the role of inflammatory process in retinal degeneration, and how to control them is the core business of our experimental work.
Annual Review 2013 27 PROCESSING INFORMATION IN THE BRAIN
One of our investigations examines We are also investigating the use of Eccles Institute sensory pathways with the long-term view light therapy and nanofibre scaffolds for of developing a sensory prosthesis. Our promoting recovery following peripheral of Neuroscience future goal is to one day decode sensory nerve and spinal cord injuries. We have information from below a spinal cord demonstrated that the use of red light lesion and artificially relay this to higher (670 nm) following contusion to the Neural Control Systems brain centres where sensory perception spinal cord can improve a variety of Laboratory could be experienced in the absence of parameters following spinal cord injury intact spinal pathways following injury. such as the improvement of behavioural Dr Jason Potas We aim to decode electrical signatures and electrophysiological outcomes, The central nervous system is a in the brainstem to extract information the promotion of anti-inflammatory cell command centre that controls that can tell us what part of the body is populations, reduction of cell death various aspects of our everyday lives experiencing sensory input as well as the and increasing neural sprouting. In using autonomic and conscious quality or nature of that sensation. The collaboration with Engineering (ANU), control systems. We are interested figure below demonstrates a functional we have developed novel nanofibre in understanding how information is map generated by recording responses scaffolds that have been able to alter coded, relayed and processed by the at different locations (black circles) on the the inflammatory response following peripheral and central nervous systems, surface of the brainstem that were evoked peripheral nerve injury. Not only does this and how this information is utilised and by stimulation of two different nerves. We have potential therapeutic value, but it integrated. Also of interest are how believe these electrical signatures can also offers novel ways to investigate the these systems are affected by injury be decoded to predict the anatomical role on inflammation in nervous tissue and disease, how they adapt or are location and sensory quality of the source recovery. modified and the mechanisms involved. input. If successful, we will be able to jcsmr.anu.edu.au/research/eccles- A better understanding of how the brain extract information from these signatures institute-neuroscience/neural-control- processes information will provide us to tell us what part of the body is systems with new strategies for tackling the experiencing sensory input as well as the Sural nerve evoked response 2 3 challenges associated with neural injury quality of that sensation. While a tactile Peroneal nerve evoked response and disease as well as provide us with sensory prosthetic device is currently 1.0 mm new insight into designing novel artificial futuristic, data obtained from our basic 1 Obex dorsal surface of medulla control systems. research is improving our understanding 4 of sensory information processing. 9 1 2 3 4 5 8
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28 The John Curtin School of Medical Research HOW EVOLUTION SHAPED PHOTORECEPTORS AND THE RETINA
of a mutation in the gene for a power within the retina, and dedicated Eccles Institute conventional G protein-coupled visual processing regions in the brain. receptor. >> In this way, the earliest fishes already of Neuroscience >> By the time that deuterostomes (our had eyes remarkably similar to those lineage) diverged from protostomes of modern vertebrates. Indeed, the Visual Neuroscience Group (the majority of invertebrates), around subsequent evolutionary changes to 600 million years ago, two distinct vertebrate eyes that have occurred Professor Trevor Lamb classes of photoreceptor, called C over the last 400–500 million years (ciliary) and R (rhabdomeric) had can be viewed as ‘fine-tuning’ of an In order to understand the structural evolved. Both classes are still present already excellent pair of visual organs. arrangement of the vertebrate retina, in almost all animals living today. and why it appears to be built ‘inside- Professor Lamb’s synthesis has permitted >> In the retinas of chordates (animals out’, with its photoreceptors on the a deeper understanding of why our retina with a dorsal nerve cord), the C side furthest away from the light, it is is arranged in the way that it is. Moreover, photoreceptors became cones, necessary to appreciate how the eye it suggests possible future experiments to and later also produced rods. In evolved. test and refine this astonishing scenario. contrast, the role of R cells changed
Professor Trevor Lamb has collected and in chordates, so that they became Early chordate Microvillar PRC analysed a broad array of findings that the retinal ganglion cells that transmit A Ciliary PRC bear on this issue. The findings that he signals to the brain. Pigment cell synthesised came from diverse fields: >> By about 550 million years ago, the Tissue expansion the molecular genetics of opsin genes, ancestors of vertebrates had evolved B C View of eye vesicle and of the genes for phototransduction a pair of light-sensitive organs on from the exterior Vitreal surface (the process that transforms captured either side of the head, but these of the retina light into neural responses); the organs were not yet ‘eyes’, as they embryological development of the did not possess a lens. Instead, they retina; the comparative morphology of probably played a ‘time-keeping’ role, photoreceptors and retinas across many reacting to cycles of day and night, Groove Early vertebrate under optic stalk living species; and, finally, evidence from just like the pineal organ does in the fossil record. today’s non-mammalian vertebrates. Scenario for the evolutionary changes that converted a light-sensitive region in an early chordate animal The results of this far-ranging work, >> By 500 million years ago, in the (similar to the living Amphioxus) into paired infolded published in Progress in Retinal and Eye lateral retinas and a dorsal pineal organ in an ancestral earliest vertebrates, those organs had Research, support the following scenario vertebrate, more than 500 million years ago. evolved a lens and had become true for the evolution of our photoreceptors eyes. In addition, numerous other jcsmr.anu.edu.au/research/eccles- and retina: changes had occurred, such as the institute-neuroscience/visual- >> The first rhodopsin arose a little development of extra- and intra-ocular neuroscience over 700 million years ago, a result muscles, increased computational
Annual Review 2013 29 Department of Genome Biology Head of Department Professor David Tremethick
Group Leader Alternatives to Animal Research through Associate Professor Brett Lidbury Bioinformatics Group Chromatin and Transcriptional Regulation Group Professor David Tremethick Computational and Conceptual Biology Group Professor Jill Gready Computational Genomics Group Associate Professor Gavin Huttley Genome Diversity and Health Group Professor Simon Easteal Genomics and Predictive Medicine Group Associate Professor Mauricio Arcos- Burgos RNA Biology Group Professor Thomas Preiss
30 The John Curtin School of Medical Research ALTERNATIVES TO ANIMALS IN BIOMEDICAL RESEARCH VIA THE INTEGRATION OF THE COMPUTER AND THE LABORATORY
desired research outcomes, the group algorithms to identify data networks that Department of has expertise in advanced statistics and in detect potential CFS/ME biomarkers, silico machine learning, as well as virology, to assist diagnosis and monitoring, and Genome Biology laboratory medicine and molecular furthermore, enhance prediction of CFS/ genetics. This style of investigation will ME severity and outcome. The Alison also rely upon biological validation in the Hunter Memorial Foundation (AHMF) Alternatives to Animal laboratory, thus blending in silico methods provided funding support. Research through with “wet lab” results and genetic In summary, AARB advanced fundamental Bioinformatics Group data, and hence developing a systems biomedical science without reliance on approach. animals, or products derived directly Associate Professor Brett Lidbury Of particular note to overcoming human from animals. Specific aims include the AARB was established to develop complexity, AARB uses sophisticated deeper understanding of pathology, and alternative methods and strategies to machine learning techniques, for example as an applied aspect to such fundamental replace animals and animal products, support vector machines (SVM), on discovery, advance medical diagnostics while achieving health progress through human data (Figure 1). This algorithm and disease prediction. In the lead up to fundamental biomedical research. allows the modelling of spaces between 2014, AARB also established the JCSMR As required by alternative research points (kernels) in high dimensional space, research support unit Innovative Methods approaches, the nature of our research providing a powerful method for detecting and Alternatives to Animal Research. is focused entirely on human biology disease or infection associated patterns Dr Alice Richardson is a visiting Fellow and disease, with the inherent challenge in complex data. In concert with SVMs, at JCSMR and from June to December of how to overcome the variation and recursive partitioning, or “tree” methods, 2013 she was present in person at the diversity of humans while pursuing are also used to untangle human data. School, being on study leave. Her home biomedical discovery. The development of a possible animal institution is the Faculty of Education, Our primary research interests include replacement alternative for the study Science, Technology & Mathematics at questions on infectious disease, of CFS/ME was completed during late the University of Canberra. She is an particularly the genetic basis of resistance 2013, and combined a human volunteer Assistant Professor in the Mathematics or susceptibility to disease post-infection, study with the pattern recognition and & Statistics Academic Program. During the role of infection and immunity in statistical methods described above. her time at the School she contributed to chronic fatigue syndrome (CFS/ME), as An experimental cohort that fulfilled the the research efforts in the Alternative to well as issues in laboratory medicine Canadian Criteria for chronic fatigue, Animal Research through Bioinformatics associated with enhanced diagnosis and and a non-fatigued control cohort, was group (Genome Biology), hosted by assay redundancy. recruited for this study. From these human Associate Professor Brett Lidbury. cohorts pathology blood test results, As an alternative to animal models, jcsmr.anu.edu.au/research/genome- genetic, serum cytokine, gut microbiology the group relied primarily on pattern biology/alternatives-animal-research- and participant clinical data were recognition methods from the field of through-bioinformatics collected. This extensive data collection knowledge discovery, applied to mass was interrogated via machine learning human data sources. To obtain the
Annual Review 2013 31 THE EPIGENETIC CODE: THE LINK BETWEEN CHROMATIN STRUCTURE AND FUNCTION DURING DEVELOPMENT
function but is also dependent upon the that lacks this acidic patch which we Department of composition of the underlying nucleosome. have designated H2A.Lap1 (Lack of Genome Biology The key way the biochemical composition an acidic patch), and consistent with of the nucleosome is altered by the our model, incorporation of this variant substitution of one or more of the core into nucleosomes inhibits chromatin Chromatin & Transcriptional histones with the corresponding histone condensation, in contrast to H2A.Z. variant. Our studies have focused on a Excitingly, using a high-throughput, deep- Regulation Group major essential variant of H2A, H2A.Z, sequencing approach, we have identified and have demonstrated that one of its this histone variant as a novel chromatin Professor David Tremethick fundamental functions is to maintain the component of the transcriptional start site It is now clearly established that the stability of chromosomes. Specifically, it (TSS) of active genes expressed during central regulator of eukaryotic gene maintains the integrity of the centromere spermatogenesis, including those genes transcription is the organization of the and surrounding heterochromatin where that escape X chromosome inactivation. genome into chromatin. Chromatin it assembles, surprisingly, compacted We therefore propose a novel model performs this crucial function by domains using both active and inactive whereby H2A.Lap1 coordinately regulates partitioning the genome into domains that histone modifications. Most recently, we gene expression by directly opening the are either open and transcriptionally active have discovered a new type of nucleosomal chromatin structure of the TSS at genes or closed and repressed. particle that contains not two molecules regulated during spermatogenesis. Excitingly, we also detect heterotypic Chromatin is built from nucleosomes of H2A.Z per nucleosome (as found at the H2A.Z/H2A nucleosomes at active (histones plus DNA), the universal centromere) but only one (i.e. a heterotypic promoters during spermatogenesis repeating protein-DNA complex in all nucleosome containing both H2A/H2A.Z demonstrating that there is a novel eukaryotic cells. Significantly, the tail of rather than H2A.Z/H2A.Z). Significantly, this process of histone variant selectivity each histone protrudes on the outside new type of nucleosome is highly unstable whereby either H2A.Lap1 or H2A.Z/H2A of the nucleosome and is subject to an and found at active promoters and other is targeted to a specific promoter. Genes extensive range of enzyme-catalysed open regions of chromatin. specific for spermatogenesis appear to modifications of site-specific amino acid In an attempt to provide a dynamic receive H2A.Lap1 while H2A.Z is targeted residues in response to intrinsic and link between chromatin structure and to house keeping genes. Most recently, we external signals. Functionally, it has function, our in vitro structural studies have have exciting new data implicating H2A. been argued that the combination of discovered a specific macromolecular Lap1 in a novel function, a role in testis such modifications is recognized or read determinant (an acidic patch) located on and brain-specific mRNA splicing. This by specific chromatin-binding proteins the surface of a nucleosome responsible suggests that H2A.Lap1 may not only involved in regulating transcription and/ for the ability of H2A.Z to promote regulate how the genome is expressed but or chromatin structure. However, our the compaction of chromatin and the also how it is spliced. recent work has shown that the functional formation of heterochromatin. Most readout of specific histone modifications is interestingly, we have recently discovered jcsmr.anu.edu.au/research/genome- not sufficient to control chromatin a new H2A histone variant in the mouse biology/chromatin-transcriptional- regulation
32 The John Curtin School of Medical Research A CONCEPTUAL BIOLOGY APPROACH TO UNDERSTANDING PROTEIN FUNCTION IN ANIMALS AND PLANTS
The effectiveness of this approach is alternative amino acid. This overcomes Department of illustrated by our rapid progress in current the otherwise limiting combinatorial research on improving photosynthetic problem of too many mutational Genome Biology efficiency for increased crop yield. possibilities. Photosynthesis in plants converts The program is being undertaken within Computational and energy from sunlight and carbon from a large conceptual, computer-based and atmospheric CO2 into energy-rich experimental program with collaborators Conceptual Biology Group biological sugars and O2 required for in the Research School of Biology, the all life – plant and animal. The enzyme commercial (Bayer Bioscience) and not- Professor Jill Gready Rubisco captures the CO2 into sugars, for-profit (GRDC) agbiotech industries We are interested in understanding how but paradoxically it is quite slow and and external testing facilities. Although proteins carry out their functions in the inefficient. Two approaches for improving our technology has many potential cell and how they have been sculpted by Rubisco performance in plants are applications (material crops, biofuel evolution to do so. Our findings provide re-engineering the enzyme itself and production, carbon sequestration) our the basic knowledge to understand introducing the modified gene into the current focus is on food production to proteins’ normal functions in plants and plant by genetic transformation, or by address the critical problem of global animals, their dysfunctions in disease and introducing naturally occurring ‘better’ food security. We have structured how they might be improved by protein variant Rubiscos into the plant by handling of intellectual property to allow re-engineering. breeding. concurrent commercial and public-good Rapid, free online access to huge We have developed new unique, and applications so that the new technology amounts of biological data in international complementary, technologies for both will be available at no cost to subsistence databanks – especially nucleotide and approaches, and validated them in farmers in lesser-developed countries, protein sequences and structures– as plants. Both use the conceptual biology assisting in improving food security and well as multiple sources of scientific approach to exploit available sequence reducing poverty. information provide new means for posing and functional knowledge, modern jcsmr.anu.edu.au/research/genome- and answering research questions. capabilities for gene sequencing and biology/computational-conceptual- We employ an approach, christened other molecular and cellular methods, biology conceptual biology, which integrates data the vast resources in seed banks and and information to create hypotheses supercomputing power. For example for and initially test them. In essence, we Rubisco re-engineering, we combined re-purpose results generated by others, insights from computational studies of and create novel links (“maps”) between the enzyme’s reaction mechanism with them. We then interrogate the combined phylogenetic analyses on public structural data “matrix” to make predictions of and sequence data, to make precise predictions of which protein residues protein structure and function for test by External testing of the mutant model plants at experiment and computation. (amino acids) to mutate and to what The Plant Accelerator facility in Adelaide.
Annual Review 2013 33 THE EVOLUTION OF HUMAN DIVERSITY AND ITS IMPACT ON HEALTH AND DISEASE
The extensive changes to our biology By studying variation in DNA sequences Department of and psychology brought about by the in human genomes we characterize complex evolutionary dynamic between how evolutionary forces, such as natural Genome Biology our ancestors and their environments selection, have shaped it. We use this have resulted in vulnerabilities, trade- information to design studies of how offs and imbalances, which now affect the evolutionary history of genetic Genome Diversity and our health and make us susceptible to variation influences how it contributes to Health Group disease. physiological and psychological diversity In our research we ask ‘Why are people and affects mental and physical health, Professor Simon Easteal so different from each other?’ ‘How have particularly in old age. As humans, we have experienced these differences between people arisen jcsmr.anu.edu.au/research/genome- substantial evolutionary change over during human evolution?’ and ‘How biology/genome-diversity-health the past few hundred thousand years. do these differences affect health and Our social and cultural environments susceptibility to disease?’ Our overall aim is have played a predominant role and to develop better approaches to managing psychological and neurological change health and intervening to prevent disease has been particularly important. One of the that incorporate knowledge of our peculiar surprising features of this process is that it evolutionary past and the biological and has led, not to an optimal ‘type’ of human, psychological differences between people but to extensive physical, psychological and that it has produced. cognitive diversity. Some of this diversity has evolved by natural selection, but some of it is an unintended side effect of the evolutionary process.
34 The John Curtin School of Medical Research THE EVOLUTION OF HUMAN DIVERSITY AND ITS HUMAN DISEASES WITH GENETIC PREDISPOSITION IMPACT ON HEALTH AND DISEASE
loci associated to devastating conditions Department of and provided the framework for defining biomarkers, prognostic assays, and Genome Biology personalized treatment options for ADHD, AD, MDD, and Membranous Translational Nephropathy. Our mission is to create a wide spectrum Genomics Group of clinical, demographic, and genomic data about complex disorders to improve Associate Professor human health. Our values are guided by Mauricio Arcos-Burgos Dobzhansky’s outline of genetic diversity The goal of our research is to identify and human equality, transparency, equal genetic variations underpinning footing, and maximal networking between inherited disorders as a tool of genetic- researchers and participants. Our vision epidemiological intervention. Our hypothesizes that genomic information in studies led to the discovery of genes/ the context of our everyday to day living loci predisposing to several complex will be fundamental to maximize your life genetic disorders, i.e. attention deficit/ quality and expectations of wellbeing. hyperactivity disorder (ADHD), Alzheimer's http://jcsmr.anu.edu.au/research/ disease (AD), membranous nephropathy, genome-biology/genomics-and- non-syndromic cleft lip with or without predictive-medicine cleft palate (NS-CL/P), major depressive disorder (MDD), among other mendelian disorders. We do maintain a large international collaboration, involving research groups from the Americas, Europe, and Australia, providing a unique source of genomic, clinical, environmental, and demographic information from thousands of patients of different ethnicities. This body of collaborative work described new genes/
Annual Review 2013 35 THE DISCOVERY OF RNAMERICA
messenger RNAs. This surprising addition ribosomes, and is aided by numerous Department of of a whole new ‘continent of noncoding accessory factors. Control at this RNA’ to the map of our genome is translational level and its dysregulation is Genome Biology somewhat reminiscent to Columbus’ increasingly recognised as an important discovery of America while searching for a factor in human health and disease. RNA Biology Group new sea route to Asia (see figure). Pathologic mechanisms may perturb The RNA Biology Group is studying the the entire cellular translation program or Professor Thomas Preiss role of coding and noncoding RNA in some specific aspect of it, leading to, for instance, malignant cell proliferation in Our genome is a complex machine the process of ‘reading’ the information cancer and inappropriate cell death or churning out a myriad of different RNA contained in our genes and interpreting growth in cardiac disease. molecules representing short-lived it to build and maintain a functioning ‘working’ copies of individual genomic organism. At the core of this process is The RNA Biology Group further engages DNA segments, while the genome itself the ‘translation’ of the code of messenger in collaborative research with medical is kept safely in the nucleus of each RNAs into proteins. The RNA Biology scientists around the world. They apply of our cells. A subset of these RNA Group studies the mechanisms and their RNA Biology in the elucidation of molecules, called messenger RNAs, patterns of this mRNA translation and processes such as the generation of encodes information for the production its regulation by RNA-binding proteins induced pluripotent cells for stem cell of proteins, the entities in cells that carry and noncoding RNAs as a means of therapy, or study the molecular pathology out most of the cellular functions. Overall, controlling gene activity. These studies of important human diseases such as the flow of genetic information from DNA continue a long tradition that began cancer, diabetes and obesity, Alzheimer’s via RNA to protein is called the ‘central with ground-breaking work performed and heart disease. dogma’ of Molecular Biology and is key by ANU scientists John Shine and Lynn Dalgarno in 1975. Translation takes jcsmr.anu.edu.au/research/genome- to all life on earth. One of the motivations biology/rna-biology of the Human Genome Project was place on cellular nano-devices called then, to discover the additional protein coding genes that separate us from other species and might explain our unique human characteristics. Surprisingly, such genes were not found and instead new research has brought to the fore that most regions of our genome actually do not carry information for the production of proteins but instead give rise to ‘noncoding’ RNAs, many of which are probably regulating the expression and utilisation of the protein-coding genes and
36 The John Curtin School of Medical Research Annual Review 2013 37 Department of Immunology Head of Department Professor Chris Goodnow
Group Leader Cancer and Vascular Biology Group Professor Chris Parish Cell Biology Laboratory Professor Chris Parish Diabetes/Transplantation Dr Charmaine Simeonovic Immunobiology Laboratory Immunogenomics Group Professor Chris Goodnow Immunogenomics Bioinformatics Laboratory Dr Thomas Dan Andrews Immune Tolerance & Signalling Laboratory Professor Chris Goodnow Ramaciotti Immunization Genomics Laboratory Dr Anselm Enders Infection and Immunity Group Associate Professor Guna Karupiah Host Defence Laboratory Associate Professor Guna Karupiah Inflammation and Viral Immunopathology Laboratory Dr Geeta Chaudhri Molecular Mucosal Vaccine Immunology Group Dr Charani Ranasinghe Translational Research Unit Dr Matthew Cook (jointly with Canberra Hospital)
38 The John Curtin School of Medical Research NEW APPROACHES TO CONTROLLING CANCER AND AUTOIMMUNITY
patients. The Group has also shown vaccines completed a promising Phase Department of that platelets play a crucial role in cancer I clinical trials in melanoma patients in spread (metastasis) and represent a 2011 and is being further perfected. Immunology target for the development of new anti- Finally, basic immunological research by metastatic drugs. Heparanase inhibitors the Cancer and Vascular Biology Group have also being investigated as potential has resulted in important advances. First, Cancer & Vascular new anti-inflammatory agents and have a blood borne protein, called histidine- Biology Group been shown to control the development rich glycoprotein, has been identified of Type 1 diabetes in mice. The diabetes which may play an important role in Professor Christopher Parish studies have also revealed that heparan controlling autoimmune diseases, such as The Cancer and Vascular Biology Group sulfate is expressed at extraordinarily systemic lupus, by aiding the elimination is particularly interested in identifying high levels by the insulin-producing cells of dead and dying cells. Second, the novel approaches to controlling cancer in the pancreas and is, in fact, essential Group has discovered a novel form of and autoimmune diseases, such as Type for the survival of these cells. Indeed, membrane exchange between cells I diabetes. When primary cancers arise loss of heparan sulfate from the insulin- of the immune system, which has the they cannot grow beyond 1-2 millimetres producing cells is associated with both potential to dramatically enhance the in diameter unless they induce the growth Type 1 and Type 2 diabetes, although immune response to both pathogens of new blood vessels that supply them via different mechanisms, immune cell- and cancer. Third, recent studies suggest with oxygen and nutrients and remove derived heparanase clearly being involved that heparan sulfate, expressed at high toxic byproducts of metabolism. Similarly, in heparan sulfate loss in Type 1 diabetes. levels by a small subset of stromal cells blood vessels play an important role in the These studies have raised the interesting in the thymus, plays a crucial role in T cell spread of cancer cells to other organs, possibility that heparanase inhibition/ development in the thymus. the cancer cells having to use a range of heparan sulfate replacement therapy jcsmr.anu.edu.au/research/immunology/ degradative enzymes to digest their way could be used as a treatment for Type cancer-vascular-biology through blood vessel walls, particularly 1 and Type 2 diabetes. In fact, heparan when they lodge in distant organs. sulfate-like drugs have been developed that can inhibit diabetes progression and The Cancer and Vascular Biology Group are being prepared for clinical trials in has been able to develop relatively simple Type 1 diabetes patients. sugar-based drugs that inhibit blood vessel growth in tumours and block a In parallel studies the Group is key enzyme, called heparanase, that investigating, in collaboration with the degrades the complex sugar molecule biotechnology company Lipotek, a heparan sulfate and aids cancer spread number of clever vaccination strategies (metastasis). One of these drugs that stimulate the immune system to (Muparfostat) is being currently tested recognise cancer cells as foreign and in a Phase III clinical trial in liver cancer eliminate them. One of these cancer
Annual Review 2013 39 BETA CELL FAILURE IN TYPE 2 DIABETES IS CHARACTERIZED BY LOSS OF INTRACELLULAR CORE PROTEINS
is essential for their survival. Our studies leading to diminished HS synthesis and Department of have also shown that normal mouse islet beta cell failure. These findings constitute beta cells strongly express a number of a new paradigm for the molecular Immunology intracellular HSPG core proteins (including processes leading to beta cell death in collagen type XVIII, syndecan-1 and T2D. HS replacement using HS mimetics CD44). This panel of HSPGs therefore early in T2D disease could potentially play Diabetes/ Transplantation represents the source of HS inside a vital role in preserving beta cell survival, Immunobiology Laboratory beta cells. The intracellular localisation thereby preserving the insulin-secreting of HSPG core proteins in beta cells is function of islet beta cells and obviating Dr Charmaine Simeonovic particularly unusual, because collagen the need for insulin therapy. Beta cell failure in Type 2 diabetes is type XVIII is conventionally localised in characterized by loss of intracellular extracellular matrix i.e., not inside cells, heparan sulfate (HS) and heparan sulfate and syndecan-1 and CD44 core proteins proteoglycan (HSPG) core proteins are normally found on the cell surface. HS is a complex polysaccharide which T2D in the db/db mouse, like T1D, is is synthesised directly onto HSPG characterised by a significant loss of core proteins. Type 2 diabetes (T2D) intra-islet HS. In db/db mice, however, is a “metabolic syndrome” disease the intra-islet levels of the HSPG core characterized by insulin resistance, proteins for collagen type XVIII, CD44 Normal db/+ islet lipolysis and hyperglycaemia. To and syndecan-1 also show a significant compensate for insulin resistance, decline prior to overt obesity and the pancreatic islet beta cells substantially onset of hyperglycaemia. These findings increase their production of insulin, raise the possibility that beta cell death inducing endoplasmic reticulum stress in T2D may result from a defect in HS (ER stress). To relieve ER stress, the synthesis, primarily resulting from a unfolded protein response (UPR) decline in HSPG core protein synthesis. pathway dampens protein production, In vitro we have found that the induction Diabetic db/db islet exacerbating insulin insufficiency. Failure of ER stress in MIN-6 beta cells (a cell Alcian blue histochemical staining shows (a) extensive of the UPR pathway to compensate for line), using treatment with thapsigargin, intra-islet HS in a normal db/+ pancreatic islet and (b) ER stress ultimately results in beta cell also leads to a significant decline in the low levels of intra-islet HS in a diabetic db/db mouse. death, necessitating insulin therapy in intracellular levels of HSPG core proteins jcsmr.anu.edu.au/research/ humans. and a significant increase in apopotosis. immunology/cancer-vascular-biology/ diabetestransplantation-immunology We have previously demonstrated that Our studies indicate that unlike T1D normal insulin-producing islet beta where beta cell HS is degraded by cells in the pancreas contain very high heparanase, ER stress in T2D down- intracellular levels of HS, a property which regulates HSPG core protein levels,
40 The John Curtin School of Medical Research UNDERSTANDING THE GENETIC BASIS OF DISEASE
High performance computing now has a Two project areas that are a current Department of central place in biological science, and we focus of our activities are analysis of are biologists-turned-computer-scientists a) human genomes to identify genetic Immunology that control and analyse genomic data determinants of autoimmune diseases, to create information. Our team has a in collaboration with our colleagues multidisciplinary background and draws in the Immunogenomics Laboratory, Immunogenomics experience from large, international and the recently established Centre Bioinformatics Laboratory public genome projects. Our systems for Personalised Immunology, and run in a high-performance computing b) matched tumour and germline Dr T Dan Andrews infrastructure provided by the National human genomes to identify genetic A data revolution is underway in Computation Infrastructure, based in part changes associated with melanoma, in modern biological science, now that at the ANU campus. collaboration with the Melanoma Institute obtaining the genome sequence for Our initial experience with high- of Australia and BioPlatforms Australia. an individual organism has become throughput genome sequence data Our focus remains on developing routine and increasingly affordable due derives not from human genomes, but information systems that allow to exponential advances in sequencing almost 2000 individual genomes of processing of, often massive, genomic technologies. As biology, and the mouse. Through analysis of these data to produce targeted information increasingly medicine, become data- mouse genomes, we have gained an to better understand the genetic basis rich disciplines, the challenge becomes undertanding of the genetic basis of of disease. We are collaborative by how to use this abundant information dozens of mouse diseases that provide a nature, as our work is a component to produce knowledge. A great many model for human diseases. of large, multidisciplinary projects that human diseases, including cancer, have Our work with the mouse genome has bring together researchers working in a genetic basis - which will be better medicine and genomics, data scientists, understood through mining of genomic proven a good training ground for our current work with human genomes. computational biologists, clinicians and information. Understanding the molecular patients. pathogenesis of these diseases – and the The genome of the laboratory mouse is new and precision treatments of these almost identical between different mice, jcsmr.anu.edu.au/research/immunology/ diseases - will be the major dividend of however, the genomes of people differ immunogenomics/immunogenomics- the genomic era. considerably from person to person – and bioinformatics how we all differ genetically from one another explains much about our differing susceptibilities to disease and cancer.
Annual Review 2013 41 IMMUNE REGULATION
Highlights in 2013 include the discovery by Another 2013 highlight was the discovery Department of PhD student James Wang with Research by Research Fellow Stephen Daley that Fellow Dr Keisuke Horikawa that common a massive number of T lymphocytes Immunology mutations in human lymphoma re-wire the developing in the thymus gland are way B lymphocytes respond to normal normally culled in two sequential waves components of our body. Normally, B because their receptor for antigen (“T Immunogenomics Group cells carrying antibodies that react with cell receptors, TCR) binds too strongly self – so-called “autoantibodies” – are to normal self-components. Until now, Professor Chris Goodnow stimulated to commit cellular suicide, evidence that we avoid developing The group discovers molecular and cellular ensuring the immune system does not autoimmune diseases by clonal deletion mechanisms and genes regulating the attack our own body. But when these of self-reactive T cells has rested on study immune response: B cells acquired a mutant form of the of individual TCRs with narrow properties, but it had not been possible to measure >> to prevent autoimmunity against self MYD88 gene, as often occurs in the antigens (eg lupus, diabetes, rheumatoid development of human B cell non- the extent, stage and mechanism of clonal arthritis, thyroiditis) and allergy; Hodgkin’s lymphoma and Waldenstrom’s deletion in the normal immune repertoire Macroglobulinemia, the “forbidden clones” comprising millions of different TCRs. >> to prevent uncontrolled growth of switched their response: instead of dying Published in a 17 page landmark article lymphoid malignancies (leukemia, they responded by growing, copying in the Journal of Experimental Medicine, lymphoma and myeloma); themselves over and over. However James Dr Daley revealed a way to do this by >> to promote immunity and and his colleagues discovered that nature measuring each of the millions of cells for immunological memory against viruses has evolved provisions for this violation their expression of a protein, Helios, that is and bacteria (immunization and of the normal rules of cell behaviour: the selectively induced when their TCR binds immunological memory). effects of MYD88 mutation are rapidly too strongly to self. This revealed that We aim to answer central questions about countermanded by cell suicide. Only when one third of maturing thymus T cells were the mechanisms of tolerance and immunity the suicide process is corrupted by a culled for self-reactivity when they were in B and T cells, and to create a collaborative second commonly occurring lymphoma semi-mature, in line with earlier predictions, environment where the next generation of mutation, such as inactivation of TNFAIP3 but that over half were culled at an earlier scientific leaders can establish independent or enforced expression of BCL2, does stage when they had first tested their TCR lines of research while drawing upon shared the MYD88 mutation fully switch the way and established it would fit the body’s expertise and resources. To achieve these the cell responds to self. Published in histocompatibility molecules, because the aims, we develop and use state-of-the-art the prestigious Journal of Experimental fit with self was too strong. These findings methods from mouse and human molecular Medicine, these findings illuminate how reveal how hard the immune system must genetics and genomics, biochemistry the body normally avoids autoimmunity, work to avoid autoimmune diseases like and cell biology, cell signalling, cellular how lymphoma develops, and a potential Type 1 diabetes, and open up new ways to immunology and clinical immunology. explanation for the co-occurrence of understand how disease-inducing T cells autoimmune disease and lymphoma. slip through these safeguards. jcsmr.anu.edu.au/research/immunology/ immunogenomics
42 The John Curtin School of Medical Research STUDIES OF IMMUNISATION AND INFECTION
Despite these efforts, key steps required We also discovered that a protein (called Department of to shape the mature B-lymphocyte SPPL2A) of previously unknown in vivo compartment are yet to be discovered. function is essential for the normal survival Immunology To identify novel genes and pathways of mature B cells. At the moment we try in B-lymphocyte development and to understand what causes the survival function we screen mice after random defect and if this dependence of mature B Ramaciotti Immunization ENU mutagenesis for defects in the cells on SPPL2A could be used for future Genomics Laboratory development of B-lymphocytes, or the treatments of B cell malignancies or B generation of an immune response cell mediated autoimmune diseases. A Dr Anselm Enders after immunization. Once a strain is further focus of the lab is the investigation The Ramaciotti Immunization Genomics identified we find the causative mutation if genes identified in the ENU screens Laboratory was established in 2008 by sequencing all exons in an affected contribute to the disease of patients through a Major Initiative Award from the animal. Over the last few years this with immunodeficiencies. We also try Clive and Vera Ramaciotti Foundation to approach has led to the establishment to better understand the phenotype of Dr Anselm Enders and Professor Chris of more than 30 different strains, and the patients by careful analysis of appropriate Goodnow. The laboratory focuses on unexpected discovery that a defect in a mouse models. These studies are done elucidating the genes and mechanisms widely expressed transporter of specific in cooperation with clinicians from the controlling the development and function phospholipids called ATP11C leads to University of Freiburg, Germany. of the immune system with a special a defect in the early development of jcsmr.anu.edu.au/ramaciotti- focus on B-lymphocytes. Normal B-lymphocytes. Further defects found immunization-genomics development of B-lymphocytes and in these mice included a defect in the the production of antibodies by them is survival of red blood cells and also the essential for protection against recurrent development of liver tumors. We are infections, and the protection provided currently following up on this discovery by most currently employed vaccines. to identify the molecular pathways that Any defect in the genetic control of are dependent on the phospholipid B-lymphocyte development or function composition of the cell membrane. can lead either to immunodeficiency, autoimmunity or B cell malignancy. Because of this importance, the generation of B-lymphocytes is one of the most thoroughly studied mammalian cell developmental systems.
Annual Review 2013 43 NEW STRATEGIES TO FIGHT VIRAL DISEASES
Much of our current understanding of the We are using a systems biology approach Department of induction and maintenance of long-lived to understand immune regulatory antibody response is based on studies networks in antiviral immunity. Novel Immunology using non-replicating model antigens. regulators of the innate and adaptive We are therefore using a well-established immune responses to poxviruses are model of orthopoxvirus infection in being elucidated using forward genetics, Host Defence Laboratory its natural host to characterize critical ENU mutagenesis and systems biology. features of long-term humoral immunity The aim is to reveal the signaling and Associate Professor to virus. We are defining the attributes of gene regulatory networks that underpin Guna Karupiah viral antigen that elicit the most efficacious the immune response to infection. Our research program uses mouse antibody responses, and which lead to The overall aim of our research program models of viral infections, which are optimal induction and maintenance of is to gain a better understanding of the validated to predict human immune long-term humoral immunity. immune response to viral infections, mechanisms, to study both innate and As the outcome of an infection is which will be key to developing novel adaptive immunity to infection. Such dependent on the interaction between therapeutic and vaccination strategies to an approach enables us to establish viral and host genes, we are also combat viral diseases. strong causal relationships and map the investigating how some virus-encoded requirements for protection against viral jcsmr.anu.edu.au/research/immunology/ immune evasion molecules subvert or infections in a way that is not possible infection-immunity/host-defence dampen the host antiviral response. in humans. We are studying innate, These studies will not only provide a cell-mediated and antibody responses better understanding of the workings of to both primary and secondary viral the immune system but will potentially infection with the aim of determining the pave the way for utilization of recombinant correlates of protective immunity. This virus-encoded proteins to treat certain is done using wildtype and recombinant chronic inflammatory diseases. viruses (orthopox, orthomyxo, herpes) in combination with wildtype, gene We are investigating the contribution knockout, gene knockin, TCR transgenic, of actin-based motility to (i) virus BCR transgenic and ENU mutant pathogenesis and (ii) viral spread within mouse models. This year we have made a natural host. We are targeting the progress in a number of areas, of which Abl-family kinases to assess these four are highlighted. processes. Our goal is to assess whether well-established anti-cancer drugs can be used as antiviral agents.
44 The John Curtin School of Medical Research THE RACE TO FIGHT INFECTION
Department of It is well accepted that both resident and receptors (TNFR) bind to membrane TNF. recruited macrophages are involved in Of the strategies used by viruses to Immunology initiating and maintaining inflammation attenuate the host response, many are in response to infection. More recent directed toward interfering with TNF studies point to a role for these cells in function, thus implicating this cytokine Inflammation and Viral regulating the switching off mechanisms as an important player in virus-host in the resolution of inflammation, once interactions. Poxviruses, including Immunopathology infection has cleared. Thus resolution ectromelia virus, encode molecules Laboratory of inflammation and return to tissue homologous to TNFR. Using this homeostasis is now thought to be an model, we have found that host TNF is Dr Geeta Chaudhri active and coordinated process which up regulated during infection and that terminates the processes involved Our research is directed towards a better membrane TNF plays a role distinct in the inflammatory response. There understanding of virus-host interactions from soluble TNF in the response to is now growing evidence that the and the immune response to infection infection. This is due, in part, to the ability of macrophages to modify their with a view to develop more effective ability of membrane TNF to participate in function based on the changing signals vaccines and selective treatments that reverse signalling. Further, virus encoded from their environment, and switch would minimise the damaging effects of an TNFR utilises the same pathways from M1 to M2, is an essential step established infection. We are pursuing this as soluble host TNFR to modulate in the resolution of inflammation and goal using a range of viral (for example, immunopathogenesis, during this pathology. We are investigating this pox and influenza A) and animal models. infection. process and the mechanisms involved in Our studies allow us to dissect the roles immunopathology as well as resolution of We are investigating the possibility of leukocyte subsets, cytokines, antibody viral disease. that recombinant viral proteins, which and a number of signalling molecules in have been engineered by nature viral infection and disease. The immune A major area of focus has been the through evolution to limit the immune effector mechanisms that are generated cytokine tumour necrosis factor (TNF); response and inflammation, may be to control and clear virus instead often expressed in both soluble and membrane used as therapeutic agents to combat cause immunopathology that has serious, forms. We have demonstrated that diseases where inflammation and sometimes lethal, consequences for the membrane TNF participates in reverse immunopathology are a problem. host. We have therefore directed our signalling, a process whereby interaction research effort toward dissecting out the with its receptor leads to the transmission jcsmr.anu.edu.au/research/immunology/ immunological parameters that allow the of signal in the direction of the cell infection-immunity/inflammation-viral- rapid resolution of virus infection with expressing membrane TNF. The biological immunopathology minimum pathology. These studies are outcomes, resulting from activation of this being carried out in parallel with others that pathway are currently being elucidated attempt to reveal the many strategies that in our laboratory and we believe it viruses have evolved to subvert the host constitutes a major route through which immune response. information transfer occurs when TNF
Annual Review 2013 45 MUCOSAL/ SYSTEMIC VACCINE AGAINST HIV-1
HIV-1 is first encountered at the genito- can transiently block IL-4/IL-13 activity Department of rectal tract, and primary CD4+ T depletion at the vaccination site and induce triple occurs in the gut mucosa. Hence, a action immunity. For example: i) excellent Immunology vaccine that can induce immunity at high “quality” mucosal and systemic these primary sites of infection (genito- CD8 T cell immunity with excellent T cell rectal tract) is thought to be the best mediated protective immunity, ii) excellent Molecular Mucosal Vaccine strategy in disease prevention. It is now gag-specific antibody class switching and Immunology Group well established that to induce sustained also iii) Env-specific B cell immunity, which mucosal immunity, a vaccine should be are hallmarks of protective efficacy in HIV Dr Charani Ranasinghe delivered to the mucosae (i.e. intranasal, controllers. We are currently assessing Globally there are approximately 34 intra rectal, oral routes). Although HIV is mechanisms of these vaccines and we million people infected with HIV-1 with the a disease of the mucosae all the HIV-1 intend to translate these vaccines into a majority of infected individuals currently clinical vaccine trials tested to date have clinical trial in the near future. living in sub-Saharan Africa and Asia, been systemic vaccine trials (delivered jcsmr.anu.edu.au/research/immunology/ reflecting the need for HIV treatment/ to the blood compartment), and these molecular-mucosal-vaccine- prevention in low-income countries. The have yielded poor outcomes in humans. immunology populations of infected individuals in Interestingly, no mucosal vaccine strategy developed countries are also considerable has yet entered into a clinical trial. with significant costs for clinical care. Our studies have revealed that mucosal Thus, it is thought that a vaccine is the delivery can induce CD8 T cells of higher best strategy and also likely the only long- quality with better protection compared term cost effective solution to contain to systemic delivery, and this is related to the HIV-1 epidemic and to reduce the the activity of cytokines IL-4 and IL-13. financial burden of the disease in both We have now developed a series of novel developing and developed countries. mucosal/systemic vaccine strategies that
46 The John Curtin School of Medical Research Annual Review 2013 47 Department of Pathogens & Immunity Head of Department Professor Carola Vinuesa
Group Leader Humoral Immunity and Autoimmunity Group Professor Carola Vinuesa The Malaria Immunology Group Associate Professor Ian Cockburn
48 The John Curtin School of Medical Research CONTROLLING THE QUALITY AND QUANTITY OF ANTIBODY RESPONSES TO PREVENT AUTOIMMUNITY
These diseases include lupus, Type 2) Identifying the genetic lesions that Department of 1 diabetes, rheumatoid arthritis, and cause autoimmune diseases in typically occur when the immune system patients. Currently, autoimmune Pathogens & cannot differentiate between invading diseases are chronic and incurable, pathogens and the body’s own cells, and existing treatments mainly consist Immunity resulting in the destruction of tissues. of non-specific inhibition of the immune Our research aims to understand system, causing serious side-effects. Humoral Immunity & autoimmune disease pathogenesis By precise identification of gene utilizing two different but complementary variants responsible for disease in each Autoimmunity Group approaches: patient, we can begin to understand disease pathogenesis, and take an Professor Carola Vinuesa 1) Unraveling the complex interactions individualized approach to therapy. Long-lasting antibody responses are between Tfh and B cells, and their regulation. Our team is deciphering Current projects are focused at identifying a key component of the mammalian rare gene variants in patients with immune system; they protect us from molecules and pathways that regulate T:B interactions and ultimately the systemic and organ-specific autoimmune the constant challenge of pathogenic diseases through exome sequencing, bacteria and viruses. The quality of the production of potent, long-lasting immunity. proving causality via CRISPR/Cas9 DNA antibodies that B cells produce matters: editing, and establishing precise mouse effective protection requires production On-going projects in the laboratory are models of each patient’s disease so we of antibodies with high affinity. Affinity focusing on the role of microRNAs, RNA- can understand how disease develops improvement occurs as a consequence binding proteins, neuropeptides, soluble and identify the most effective treatment. of random mutation targeted at the factors, and novel surface receptors genes that encode for the B cell receptor. on Tfh cells and germinal center B cell jcsmr.anu.edu.au/research/pathogens- Mutated B cells can be selected by a selection. immunity/humoral-immunity- subset of T cells known as T follicular autoimmunity helper (Tfh) cells to become long-lived plasma cells that will secrete high affinity antibody for decades. However, this ability to produce long-lasting high affinity antibodies is a double-edged sword: perturbations can result in over 80 different autoimmune diseases that collectively affect 3-5% of the population.
Annual Review 2013 49 A VACCINE TO PREVENT MALARIA
The group is focused on understanding This year the group has also begun Department of immune responses to the Plasmodium a totally new research direction - parasite that causes the disease malaria. investigating how Plasmodium-specific B Pathogens & Malaria kills 0.5–2 million people each cells and antibodies develop in response year, mainly in Africa. A safe and effective to vaccination. We are developing a Immunity vaccine is likely needed for the control or range of tools to track and analyse these eradication of this disease. Immunization cells. In the coming years we aim to use by irradiated malaria-infected mosquitoes these tools to understand how our most The Malaria Immunology has been shown to protect people promising vaccination strategies work and Group against malaria. Irradiated parasites do how we can make them better. not divide and cause infection but are jcsmr.anu.edu.au/research/pathogens- Associate Professor capable of activating the immune system immunity/malaria-immunology Ian Cockburn to make protective responses against infection. The parts of the immune system 2013 saw the establishment of the that contribute the most to protection Malaria Immunology research group are B cells, which make antibodies, and within the Department of Pathogens and CD8+ T cells – specialized cells capable Immunity. This was made possible by of killing parasite infected cells in the liver. the recruitment of Associate Professor Ian Cockburn to the school from Johns Research highlights this year include the Hopkins University in Baltimore, USA. first direct observations of T cells killing Associate Professor Ian Cockburn was parasites in the infected liver. This was joined by a post-doctoral fellow – Dr made possible by the use of intra-vital Johanna Dups – from the Australian imaging technology. Using this the group Animal Health Laboratories and a was able to begin to address a key research assistant – Yeping Cai from the question in T cell biology – how do these Department of Translational Medicine, cells find and eliminate their targets. JCSMR. It was found that CD8+ T cells were capable of forming large clusters around parasite-infected cells. Surprisingly killing appeared to be a protracted process, an observation that contrasts with data from in vitro suggesting that a single activated CD8+ T cells can rapidly kill multiple targets. The group will continue to investigate the molecular basis of our observations using a newly purchased 2-photon microscope at The John Curtin School of Medical Research.
50 The John Curtin School of Medical Research Annual Review 2013 51 Department of Molecular Biosciences Head of Department Professor Angela Dulhunty
Group Leader Biomolecular Interactions Group Dr Marco Casarotto Cancer Metabolism and Therapy Group Dr Anneke Blackburn Cardiac and Skeletal Proteomics Group Dr Nicole Beard Cytokine Molecular Biology and Signalling Group Professor Ian Young Epigenetics and Genome Stability Group Dr Danny Rangasamy Molecular Genetics Group Professor Phil Board Muscle Research Group Professor Angela Dulhunty Stem Cells and Gene Targeting Group Professor Klaus Matthaei
52 The John Curtin School of Medical Research DRUG DESIGN
pharmacology, drug design/delivery development of new therapies. We aim Department and molecular biophysics. Listed below to combine our expertise in epigenetics, are some of the projects that are being cancer, pathology, structural biology and of Molecular studied. drug design to demonstrate that various Bioscience The dihydropyridine and ryanodine enzymes are novel epigenetic regulator receptors (DHPR &RyR) play essential of human cancers, and propose a clear roles in muscle function and my and integrated translational strategy to Biomolecular contribution has been to determine treat cancers with novel small molecule how these proteins signal to each other inhibitors. Using a multidisciplinary Interactions Group in skeletal excitation-contraction (EC) approach underpinned by internationally coupling. Because the protein machinery competitive basic science, we will be able Associate Professor present in skeletal and cardiac muscle to apply our novel studies on epigenetic Marco Casarotto is very similar, it is important to identify regulation to personalised approaches The main focus of the work in my key differences in the makeup and to therapy. laboratory has been to explore the diverse function of these two systems. The 15 Virions or virus ion channels are small structural and physical properties of kD intracellular II-III loop region of the membrane proteins capable of forming biomolecules in order to gain a greater DHPR is implicated in skeletal muscle multimeric ion channels and they are understanding of the physiological roles function and we have been responsible expressed in a number of viruses they play in the body. A number of diverse for elucidating the first high resolution including influenza A (M2), HIV-1 (Vpu) structure-based strategies including structure study of this region. We and Hepatitis-C (P7). We have developed nuclear magnetic resonance (NMR) have also determined that the skeletal a strategy that employs surface plasmon spectroscopy, molecular biology, protein- b-subunit of DHPR has distinct interactive resonance techniques to measure the engineering and rational drug design properties compared with its cardiac affinity of a number of antiviral drugs to techniques are employed to address counterpart. This work has laid the these ion channels and in the case of important biological questions. A wide foundation for us to propose a series of the M2 and Vpu ion channels, have used range of medically relevant projects are novel hypotheses that are currently being this technology to elucidate the mode of carried out, all designed to provide a evaluated not only in my laboratory but binding of various drug compounds. unique insight into the role that specific world-wide. jcsmr.anu.edu.au/research/molecular- biomolecules play in a number of disease Epigenetic Enzymes activate or silence bioscience/biomolecular-interactions states. By understanding these roles it genes by epigenetically ‘tagging’ the is then possible to identify and design amino terminal tails of histone proteins novel therapeutic agents for particular and are critical molecular mediators of diseases. This work has been published a number of biological processes. The in diverse biological and chemical journals contribution of epigenetic enzymes that reflect the broad application of our in the regulation of human cancers research and encompass areas such as is an exciting area and critical to the physiology, microbiology, biochemistry,
Annual Review 2013 53 TARGETING THE SWEET TOOTH OF CANCER
for use in cancer treatment. We have Department demonstrated that dichloroacetate is able to stop the growth of metastatic breast of Molecular cancer cells both in vitro and in vivo in Bioscience animal models. While dichloroacetate alone does not kill breast cancer cells, taking away the drive Cancer Metabolism to grow makes the cells more likely to die when co-treated with existing anti- and Genetics Group cancer drugs. This could mean the dose of current anti-cancer drugs could be Dr Anneke Blackburn lowered, thus reducing toxic side effects It was first recognized in the 1930s that but without loss of effectiveness. We are cancer cells use glucose differently to currently investigating which drugs DCA normal cells. Normal cells use oxygen to works best with. metabolize glucose for energy production, whereas cancer cells preferentially We are also interested in the genetic metabolize glucose for the production factors that contribute to breast cancer of cellular building blocks via a pathway susceptibility, and in the outcomes that does not require oxygen and results for women identified with very high in the accumulation of lactic acid. Other predisposition for developing breast aspects of metabolism in cancer cells cancer. We are investigating the possible also differ from normal tissues, and role of the vitamin D / calcium pathway these differences are yet to be exploited in breast cancer in mice genetically for therapeutic purposes. The Cancer predisposed to developing cancer. These Metabolism and Genetics Group is mice carry a mutation in the tumour studying the effect of redirecting cancer suppressor gene p53 and are a model metabolism on the growth and death of of the multiple tumour syndrome, Li- breast cancer cells. We are particularly Fraumeni Syndrome. Experiments such interested in dichloroacetate (DCA), as this take several years to complete, but an old drug that inhibits the anaerobic the discovery of modifiable risk factors for metabolism of glucose to lactic acid. This breast cancer prevention would greatly drug has been used for many years in improve the outlook and quality of life patients with a rare metabolic disorder for women carrying mutations in breast and so it is known to be safe and have cancer predisposition genes. minimal side effects, and therefore has jcsmr.anu.edu.au/research/molecular- potential to move rapidly into the clinic bioscience/cancer-metabolism-genetics
54 The John Curtin School of Medical Research UNDERSTANDING HOW BLOOD CELL DEVELOPMENT IS REGULATED BY CYTOKINES: IMPLICATIONS FOR ALLERGY, ASTHMA AND LEUKEMIA
crystallography and more recently have Department determined the structure of the mouse beta-IL-3 receptor (collaboration with of Molecular Paul Carr, Colin Jackson and David Ollis, ANU and James Murphy, Walter & Bioscience Eliza Hall Institute of Medical Research, Melbourne). We are continuing with the structure of cytokine-receptor complexes. Cytokine Molecular Biology Our aim is to understand the two and Signalling Group different modes of receptor activation which influence whether blood cell- Professor Ian Young forming stem cells undergo maturation We are studying the role of three or self-renewal in response to IL-3. By hormone-like proteins (cytokines; IL-3, elucidating the mechanisms controlling IL-5 and GM-CSF) which stimulate the blood cell maturation we aim to develop expansion of inflammatory cells in asthma new treatments to control inflammation and may also have a role in leukemia. The in asthma (collaboration with Paul cytokines bind to cell surface receptors Foster, University of Newcastle) and to and influence the growth, maturation understand the involvement of the IL-3 and activation state of blood cells. In receptor system in myeloid leukemia asthma, the symptoms are caused by (collaboration with Professor Y Chen an allergic reaction in the lung and the and Dr Y Wu, Fujian Medical University, production of inflammatory cells. The China). + inflammatory cells include CD4 T helper-2 jcsmr.anu.edu.au/research/molecular- lymphocytes (Th2 cells), eosinophils, bioscience/cytokine-molecular-biology- mast cells, basophils, neutrophils and signalling macrophages, all of which have all been linked to the pathogenesis of asthma with different cell subsets predominating in specific asthma subtypes. Our aim is to elucidate the molecular details of the cytokine-receptor interactions and how these interactions initiate signals across the cellular membranes. We have previously determined the 3D structure of the main signalling receptor (hbetac) shared by these cytokines using X-ray
Annual Review 2013 55 DISCOVERY OF HOW RETROVIRAL RELICS COULD LEAD TO THE GENOMIC CHAOS OF CANCER
In contrast, it is overexpressed in patients Given the deleterious nature of Department with breast cancer. The unchecked retrotransposon activity in cancer LINE-1 activity would create havoc on development, we are interested in the of Molecular the genome, initially through insertional mechanisms by which the retroviral mutations and later by genomic relics are regulated in somatic cells. Bioscience instability through high levels of double- Using deep-sequencing technology strand DNA breaks, deletions, and combined with microarray analysis, genomic rearrangements. Although we have recently identified a class of Epigenetics and Genome the mechanistic pathways that activate small non-coding endo-siRNAs as a Stability Group LINE-1 expression are not known, its key regulator of retrovirus in the human expression and its effect on genomic genome. Currently, we are testing the Dr Danny Rangasamy instability have given rise to suggestion epigenetic role of endo-siRNAs including Breast cancer is the most common that it may serve as useful biomarkers DNA methylation and RNAi-mediated malignant disease in women. The in the early diagnosis of breast cancer silencing pathways. This research project worldwide incidence of breast cancer or in the prediction of cancer onset. will shed new light into how and why continues to rise, and has become Specifically, LINE-1 biomarker is useful disease states arise in breast tissues a leading cause of cancer deaths. in the prediction of genetic changes and how the expressions of retroviral More than 90% of breast cancers are associated with the transition from relics activate the process of cancer sporadic or acquired somatic mutations. normal to hyperplasia to insitu breast development. Unravelling the relationship It is believed that genomic chaos carcinoma to invasive breast cancer. between small regulatory endo-siRNAs might promote the accumulation of With collaboration of the Department of and DNA methylation in the development genetic changes in apparently normal Pathology, the Canberra Hospital, we are of cancers may provide new strategies breast tissue, even before histological currently testing this biomarker using a for future therapeutic and diagnostic abnormalities are detectable. Although panel of breast cancer tissues and serum approaches. genomic chaos is commonly found in samples. http://jcsmr.anu.edu.au/research/ invasive carcinomas, little is known about molecular-bioscience/epigenetics- the timing of critical changes in early genome-stability tumorigenesis or changes associated with the transition from normal cells to primary breast carcinoma. We have recently identified retroviral relics “LINE-1 retrotransposons” that are differentially expressed in normal and breast cancer tissue. In healthy tissues, the expression of LINE-1 is completely absent throughout the cell cycle.
56 The John Curtin School of Medical Research MUSCLE RECEPTOR PHYSIOLOGY
The research of the Muscle Group falls The third area is the elusive problem of Department into three areas. The first is excitation- how proteins that are located right inside contraction (EC) coupling which allows the calcium ion store can regulate RyR of Molecular an electrical signal on the cell surface activity in a manner that depends on to release calcium from its internal store the amount of calcium stored, to ensure Bioscience though the RyR. EC coupling depends that fewer calcium ions are released if on interactions between the RyR and a the store is depleted. To understand this dihydropyridine receptor (DHPR) calcium problem, we isolate the individual proteins Muscle Research Group channel in the surface membrane. In and then reconstruct the protein complex the heart this enables RyR activation by that they form with the RyR, at the same Professor Angela Dulhunty calcium ions that enter from outside the time controlling calcium concentration in The Muscle Research Group studies cell through the DHPR. In skeletal muscle the store and measuring the ionic current molecular interactions between proteins there is a depolarisation-dependent signal flowing through the RyR ion channel. that reside inside muscle cells and that transmitted from the DHPR to the RyR by come together to form a molecular http://jcsmr.anu.edu.au/research/ direct interactions between the proteins. molecular-bioscience/muscle-research machine which provides fuel in the Despite years of investigation, we still form of calcium ions to drive skeletal do not know which parts of the proteins muscle contraction and cardiac output. interact to achieve the coupling. The central protein in this machine is a calcium ion channel known as The second area is the changes in the a ryanodine receptor (RyR) which is ryanodine receptor that lead to heart and located in the membrane of a calcium ion skeletal muscle disease. There are many store deep inside the muscle cells. The risk factors for these disorders including calcium ion channel is essential for life smoking, diet, obesity and exercise as because contractile force generated by well as chemotherapy. In addition, there the muscle depends on highly regulated are risk factors that we are born with, cyclical changes in the calcium ions which include genetic changes in the RyR that are available to bind to contractile and the molecules that control its ability proteins to allow them to generate muscle to open and release calcium ions. We force. The operation of this calcium are working to understanding how these ion channel is compromised in skeletal factors impact on RyR function. We have muscle myopathies and in acquired heart discovered that a natural enzyme which and skeletal muscle disease, as well as is present in all muscle cells is a potential with genetic defects that severely affect pharmacological agent for treating heart muscle function. failure and we are refining the molecule for better therapeutic potential.
Annual Review 2013 57 Department of Translational Medicine
Head of Department Professor Julio Licinio (until April) Dr Claudio Mastronardi (from April)
Group Leader Pharmacogenomics Group Professor Ma-Li Wong Translational Medicine Group Professor Julio Licinio Translational Endocrinology Team Dr Gilberto Paz-Filho Translational Psychiatry Team Dr Claudio A Mastronardi
58 The John Curtin School of Medical Research TRANSLATIONAL MEDICINE
Our research aims at understanding the Department of endocrine-neuroimmune-gene interactions of relevance to human disease, particularly Translational obesity and depression, both as separate entities and interfacing disorders. We aim at Medicine leading by example by engaging with basic, clinical and population researchers. Because of our broad interest, we are well suited to Translational Medicine interact with researchers working at different Body weight comparison between a leptin-deficient Group levels (basic science, clinical and population (right) and leptin-sufficient mice. research), as well as private companies. Dr Claudio Mastronardi This line of work includes studies aimed after fat transplantation, by performing Dr Gilberto Paz-Filho at identifying molecular pathways and metabolic, histologic, hormonal, genomic In 2013, the Department of Translational regulatory mechanisms that are activated and flow cytometry analyses. Our model will Medicine steadily developed the areas in the brain and peripheral tissues by demonstrate the interactions between the of education, community outreach inflammatory stress. By studying the adipose tissue and distant organs, providing and research. We successfully hosted changes in gene expression in the the foundation for the development of novel educational activities at the international hypothalamus, adrenals and spleen, therapies based on fat transplantation and national levels, convening the Graduate before and after the induction of systemic against diabetes and obesity. Certificate and Master of Translational inflammatory response syndrome In our clinical studies, we aim at Medicine Programs, along with related (SIRS) through the injection of bacterial understanding how body fat, high blood courses. The Department also promoted lipopolysaccharides, we have identified glucose, high blood lipids and high blood the 4th Bootes Course on Translational genes that could aid in the development of pressure affect mood, quality of life and Medicine, with the theme Stem Cells in biomarkers or therapeutic agents against overall brain function (such as memory, logic Human Health, hosting speakers from SIRS and sepsis. and ability to solve puzzles). In addition, by the ANU, CSIRO, Monash University and By transplanting fat from normal mice into employing whole exome sequencing, we University of Melbourne, and attracting genetically obese, leptin-deficient mice (ob/ have been able to identify novel genes that close to 40 registered attendees. ob) (See Figure), we have shown that cells might be associated with severe early-onset In our community outreach initiatives, we from the implant migrate into distant organs obesity in children. In one particular case hosted a Clinical Research Open Day on of the recipient mice. This suggests that of a severely obese child, we were able 1 June, attended by almost 200 members the distant infiltration of graft-originated, to identify the first human case of a novel of the community, who learned about our leptin-producing cells contributes to the mutation in a gene that is closely related research into obesity and hypertension. improvement of diabetes and to remarkable to the regulation of body weight, through We also participated in ANU Open Day. weight loss in those genetically obese the leptin-melanocortin pathway in the We took these opportunities to expand mice. Our ongoing studies will characterise hypothalamus. public awareness, to foster avenues for the effects and nature of those adipose- jcsmr.anu.edu.au/translational-medicine philanthropic funding and to recruit research derived cells that infiltrate distant organs participants for our clinical projects.
Annual Review 2013 59 AUSTRALIAN PHENOMICS FACILITY
The Australian Phenomics Facility (APF) is The year focused on introducing a The APF continued to support the the School’s nationally accessible service systems approach and improved client Australian Phenome Bank (APB) – a organisation for creating, characterising services. Towards this end the facility centralised repository for all genetically and curating mouse models of human developed the APF Client Service Charter, modified mouse strains held in Australia disease. Biomedical researchers use the released a new unified sample storage either live or as cryopreserved material. APF’s large-scale research infrastructure database and created new reporting The associated puvblic database, now to investigate a range of biological functions within the APF’s animal containing a total of 3,499 strains, allows questions to better understand the management database. researchers to search for murine strains, genetic components underlying a wide Throughout 2013 the APF continued to housed or archived in Australia, carrying range of human diseases and access expand the Missense Mutation Library mutations in particular genes, strains with disease models for the development (MML) to a capacity of 12,833 Single transgenic alterations and for mice with of new therapies. In conjunction with Nucleotide Variants (SNVs) publically particular phenotypes, http://pb.apf.edu. the activities located in the Hugh Ennor listed and providing the Australian au/phenbank. Of these strains, 1,961 are Building the APF also manages the research community with thousands of contained within the frozen archive and after resources in the School’s containment mutant mice for use in understanding successfully implementing improved mouse suites, the Director’s Lab and the Stage 3 disease biology, databases.apf.edu. IVF techniques the team successfully re- animal facilities. au/mutations. The mutations listed on animated 65 strains from the cryopreserved During 2013 the facility continued to the MML have been integrated with sperm stock. receive Federal Government support from third-party databases to enhance their the Education Investment Fund Super content value which include Mutagenetix, Science Program and the newly created Orphanet rare diseases, novel genes, Collaborative Research Infrastructure protein domains, SIFT predictions and Scheme. PubMed. The release of the ‘Gene Watch’ feature also resulted in an expanding list of subscribers monitoring close to 1000 different genes.
60 The John Curtin School of Medical Research International highpoints came in the visits Highlights >> Facility Outreach - APF databases for to Rome, Spain, China, Singapore, Japan the MML and APB were revamped >> Colony Management - In addition and Korea where APF staff presented on with a new look-and-feel for a to regular animal care each week, the significant mouse resources available stronger online presence, resulting (managing around 9000 cages), the through the facility and associated in 2071 and 1596 unique visitors team freed up valuable researcher collaborative partners. The facility was to the websites respectively. They time by performing various tasks welcomed as a new member of the Asian also gained additional international including breeding management, Mouse Mutagenesis Resource (AMMRA) exposure through connection to other routine examinations, injections, and Phenotyping Consortium (AMPC). popular research groups websites: genotyping and sample collection, This facilitates researcher access to International Mouse Phenotyping as well as providing comprehensive mouse resources in Asia and standardise Consortium (mousephenotype. training for new students and org) and International Mouse Strain phenotyping protocols and genetic researchers utilising the facility. background controls that are crucial for Resource (findmice.org). >> Genome Sequencing - The APF comparing data from different research www.apf.edu.au laboratories. As part of the new alliance, acquired new automated high throughput equipment to remove in 2014, the APF will host the Annual pb.apf.edu.au the reliance on manual processing Meeting of the AMPC alongside presenting of samples and streamlines the the School’s 16th Frank & Bobbie Fenner enrichment of DNA libraries for the Conference “Towards precision medicine: exome sequencing pipeline. This Phenotyping human diseases in mice. equipment and was funded by an ANU Major Equipment Grant and contributions from several research groups within the ANU.
Annual Review 2013 61 THE IMAGING & CYTOMETRY FACILITY
The Imaging and Cytometry Facility cell and tissue fluorescence and light The Quantum FX micro CT is a fast, low provides access to imaging and flow microscopy. Analysis software is available dose instrument with a resolution to 10 cytometry equipment and a histology for quantification of images including microns. With fast imaging speeds as service as well as technical expertise, Imaris, Mediacy ImagePro and Auto short as 17 seconds the instrument is support and training. The facility’s Deblur and Leica Deconvolution. suited for projects with a large number services are available to all researchers This year saw the setup of a new small of samples as well as in-vivo imaging. and students and other interested parties animal imaging suite in the Facility. The Images from both instruments can be across ACT. Perkin Elmer IVIS Spectrum and Quantum easily co-registered i.e. an optical 3D The flow cytometry lab supports a wide FX micro CT were installed in April 2013. tomographic image can be merged with range of flow cytometry techniques This instrumentation was previously the micro computed tomographic image including full service multi-parametric cell unavailable in the ACT and is used for to show functional optical signals with sorting of up to 30,000 per second and non-invasive longitudinal monitoring of anatomical detail. A number of training single cell sorting as well as supported disease progression, cell trafficking and sessions have been run since April this self-service analysis of complex cell gene expression pattern in living animals. year to introduce this equipment to researchers. populations using benchtop flow The IVIS Spectrum optical imaging cytometers. The latest versions of analysis system is able to image, with high- The Histology lab is a full service, fast software, FlowJo and BD Diva, are sensitivity, in-vivo fluorescence and turnaround area with a very experienced available to use with training and support. bioluminescence at a resolution to 20 histologist who provides advice to both A 561nm laser was added this year to the microns. It features 28 high efficiency experienced researchers and novice BD Fortessa benchtop flow cytometer filters spanning the range of 430 – users. Along with routine stains and which has increased the number of 850nm, can image in epi- or trans- techniques, a large number of special parameters to 18 for this instrument. illumination fluorescence mode, create a stains and techniques are available on Further upgrades to other instruments are 3D tomographic reconstruction for both request. planned for early 2014. fluorescence and bioluminescence and http://jcsmr.anu.edu.au/research/ The imaging labs provide access to allows a high throughput of up to five research-facilities/microscopy- high end confocal and multi-photon mice per image. cytometry-resource-facility-mcrf microscopy as well as live and fixed
62 The John Curtin School of Medical Research THE AUSTRALIAN CANCER RESEARCH FOUNDATION BIOMOLECULAR RESOURCE FACILITY AND GENOME DISCOVERY UNIT
The Australian Cancer Research samples is being done at JCSMR and was expression and miRNA expression with Foundation‚ Biomolecular Resource ongoing throughout 2013. 12,000 data points per run enabling Facility (BRF) provides consultancy Other large-scale projects include large scale population studies looking at and service provision in the areas of providing sequencing for the Australian a large number of genes. For the protein genetic sequencing, peptide synthesis, Phenomics Facility at JCSMR for biologists, we now house the Millipore mass spectrometry, tetramer synthesis, identification of single nucleotide Direct Detect system which is an infrared chromatography and antibody purification polymorphisms in mutant mouse strains. based technology allowing accurate to ANU researchers, as well as the wider quantitation of biomolecular samples scientific community. The facility acquired significant new such as proteins and peptides, lipids and equipment during 2013. Major High Throughput DNA sequencing (HTS) carbohydrates. This platform is distinct Equipment Committee funds were because only small volumes of protein are continues to constitute a significant awarded for a new Illumina MiSeq. proportion of the BRF workload. The BRF required and measurements can occur in This is a benchtop next generation complex mixtures and buffers. is a service node of Genomics Australia sequencer made to have smaller output (part of Bioplatforms Australia). As part of compared with our larger instrumentation. brf.anu.edu.au this consortium, we have been sequencing Its applications include sequencing genomes and biopsies from a large small bacterial genomes, or targeted cohort of melanoma patients. We finished amplification on regions of interest, sequencing all samples in the middle of suitable for the clinical setting. On the year. Data analysis for all consortium the theme of large-scale analysis, we acquired a Perkin Elmer LabChip GX which is a high throughput microfluidics platform which can assess the quality and quantitation of DNA and RNA in a high throughput manner, being able to analyse 96 samples in less than an hour. Similarly, we were able to add functionality to one of our Realtime PCR instruments with an Open Array platform. This allows real-time PCR assays for SNP genotyping, gene
Annual Review 2013 63 64 The John Curtin School of Medical Research Staff and Student achievements
Graduates Prizes and Awards
Annual Review 2013 65 GRADUATES
PhD Sau Kuen Lee MNeuroscience (Research) Understanding Pathogenic and Mark James Abraham Physiological T Follicular Helper Cell Daniela Andrea Espinoza Oyarce Formation Challenges and Progress with Molecular Eva Pillai Dynamics Simulation of Disordered Peptides Ryan Robert Taylor Neural Computation of Statistical MTranslational Medicine Animesh Agrawal Structure Rubisco Sequence‑Structure‑Function: Zheyi Li Yang Xi Coevolution and Codon Bias of rbcL Shali Zhao Gene Adjuvant Activity of Interferon Epsilon and Toll‑Like Receptor‑9 to Enhance Long Chen HIV‑Specific Mucosal Immunity Neuroscience Honours Understanding the Mechanism that Haihan Jiao Regulates LINE‑1 Retrotransposons in MNeuroscience Breast Cancer Cells Andrew Micallef Praveen Mathew Alexander Hugh French Tanja Racic The Influence of Genetic Variation on Byunghee Choi Inducible Gene Expression Iris Zhu Kritika Toraskar Alexander Hadjinicolaou Henrik Mårtenzon Optimal Electrical Activation of Retinal Ganglion Cells Amgalan Zinamidar
66 The John Curtin School of Medical Research PRIZES AND AWARDS
Mr Hannes Bergmann Mr Daniel Chaston Dr Anselm Enders Travel award to attend the 2nd IFReC‑SIgN American Physiological Society, The inaugural Gordon Ada Early Career Winter School on Advanced Immunology, Cardiovascular Research Recognition Researcher Award – presented to an early Sentosa Island, Singapore Award career researcher at JCSMR who has Best Oral Presentation 2nd IFReC‑SIgN made major contributions to biomedical Winter School on Advanced Immunology, Ms Fui Jiun Choong research Sentosa Island, Singapore TTS/IPITA Young Investigator Travel rd Nilisha Fernando ASI Travel Bursary to attend the 43 Award to attend the 14th World Congress Annual Meeting of the Australasian of International Pancreas and Islet Best student poster presentation: 3rd Society For Immunology, Wellington, NZ Transplantation Association (IPITA) International Conference on Clinical and Monterey, CA, USA Experimental Ophthalmology, Chicago, Dr Edward Bertram Transplantation Society of Australia and IL, USA JCSMR Director’s Travel Award to New Zealand Travel Award attend the 2013 Translational Research Transplantation Society of Australia and Professor Chris C Goodnow and Entrepreneurship Short Course at New Zealand Young Investigator Book Elected as a member of the US National Indiana University – Purdue University Prize Academy of Sciences Indianapolis, Indianapolis, IN, USA
Annual Review 2013 67 Professor Jill E Gready Mr Shaun Lehmann Dr Gilberto Paz–Filho The Future Justice Prize ANU Chancellor’s Letter of The Endocrine Society Promotion and Commendation for outstanding Tenure Workshop Travel Grant Award Dr Rebecca Haddock academic performance Professor Waldemar Berardinelli Award for best Clinical Research Article Young Investigator Award: Joint Australian Dr Yalin Liao published in 2012 in the Brazilian Archives and New Zealand Microcirculation of Endocrinology and Metabolism: Society, Australian Vascular Biology Awarded The John Stocker Postdoctoral Bornschein A, Paz-Filho G, Graf H, Society Meeting, Barossa Valley, SA Fellowship 2014‑2017 de Carvalho G (2012) Treating primary Radiation Oncology Private Practice Trust Conference Travel Grant to attend Global hypothyroidism with weekly doses Fund Award: Best Laboratory Research Young Scientist Summit, One North, of levothyroxine: a randomized, Oral Presentation, Canberra Hospital Singapore single‑blinded, cross‑over study. Arq Bras Annual Research Meeting, Canberra, ACT Endocrinol Metabol 56:250‑8 Ms Susan Morton Mr Yogesh Jeelall Mr Alvin Pratama Best Oral Presentation Prize: Now Lorne Cancer Conference Travel Bursary you see it, now you don’t: Switchable Student Presentation Prize: ACT and to attend 2013 meeting, Lorne, VIC Transgenic Mice, The Australian Society NSW Australasian Society of Immunology for Medical Research (ASMR), ACT Annual Branch Retreat, Bowral, NSW Mr Mayank Khanna Branch New Investigators Forum, Canberra, ACT Professor Jan Provis JCSMR Director’s Travel Award to Best Oral Presentation involving attend the 2013 Translational Research Biochemistry (ASBMB) Prize: Now ANU College of Science Award: and Entrepreneurship Short Course at you see it, now you don’t: Switchable Outstanding Contribution to Student Indiana University – Purdue University Transgenic Mice. Learning Indianapolis, Indianapolis, IN, USA Professor Christopher Ms Jennifer Robertson Ms Candice Lee R Parish Winner: 3‑Minute Thesis competition, JCSMR Director’s Travel Award to The John Curtin School of Medical attend the 2013 Translational Research Named Director of The John Curtin Research, ANU, Canberra, ACT School of Medical Research and Entrepreneurship Short Course at Winner, 3‑Minute Thesis competition, rd Indiana University – Purdue University The 3 Annual John Curtin Lecture: ANU College of Medicine, Biology and Indianapolis, Indianapolis, IN, USA Cancer: Is a vaccine possible? Environment, ANU, Canberra, ACT Runner‑up: 3‑Minute Thesis competition, Ms Suhyun Lee The Australian National University, Invited Guest Speaker Award– Oral Canberra, ACT presentation and Press Conference through the Featured Poster Session, The Endocrine Society Meeting, San Francisco, CA, USA
68 The John Curtin School of Medical Research Dr Zahra Sabouri Dr Rebecca Sweet Dr Harpreet Vohra Poster prize: The Australian Society for American Association of Immunologists Australian Endeavour Fellowship from Medical Research (ASMR), ACT Branch Travel Grant to attend the 15th The Australian Government for travel to New Investigators Forum, Canberra, ACT International Congress of Immunology, Stanford University ‘to develop knowledge Milan, Italy and skills in flow cytometry using new Ms Tennille Sibbritt cutting edge technology currently Professor David Tremethick unavailable in Australia’ Promega Student Award for Research Excellence: Gene expression and The inaugural ANU College of Medicine, Mr James Q Wang Organisation, 34th Annual Lorne Genome Biology and Environment Prize for Conference, Lorne, VIC Outstanding Research Achievement, Lorne Cancer Conference Travel Bursary Vice‑Chancellor’s Higher Degree awarded in recognition of a particularly to attend 2013 meeting, Lorne, VIC Research Travel Grant to attend the 18th meritorious paper or papers that were Annual Meeting of the RNA Society, published in the preceding calendar year Mr Danushka K Wijesundara Davos, Switzerland The Alan and Elizabeth Finkel Prize RNA 2013 Travel Fellowship: 18th Annual Ms Shubhanshi Trivedi Meeting of the RNA Society, Davos, Travel Bursary to attend Keystone ANU Vice‑Chancellor’s High Degree Symposium: HIV Vaccines (X2), CO, USA Switzerland Research Travel Grant Award to attend the International Congress of Mucosal Dr Charmaine Simeonovic Immunology meeting, Vancouver, BC, Mr Mehmet Yabas Canada rd Transplantation Society of Australia and ASI Travel Bursary to attend the 43 New Zealand (TSANZ) Travel Award to Annual Meeting of the Australasian attend the 14th World Congress of the Dr Krisztina Valter-Kocsi Society For Immunology, Wellington, NZ International Pancreas and Islet Transplant Awarded a Senior Fellowship in The ASI Postgraduate International travel Association Meeting, Monterey, CA, USA Higher Education Academy, UK Award to attend Keystone Symposium: B Cell Development and Function, Ms Monika Srivastava Keystone, CO, USA Australasian Society of Immunology International Post‑graduate Travel Award to attend the EMBL Conference, Heidelberg, Germany
Annual Review 2013 69 70 The John Curtin School of Medical Research Visitors and Collaborations
Research Collaborations and Visitors to the School Visitors to JCSMR present their research findings through Group, Department or School Seminars and workshops, and collaborate with our staff and students in our world‑class research facilities. Staff and students at JCSMR continue to enjoy close collaborative ties with other researchers at The Australian National University. In addition, they enjoy scientific associations with colleagues locally, regionally, nationally and internationally.
Annual Review 2013 71 VISITORS
Dr John Altin Associate Professor Richard Professor Robert French Applications Development, Prognosys Callaghan Department of Physiology and Biosciences, San Diego, CA, USA Human Disease and Membrane Transport Pharmacology, Medicine, University of Laboratory, Research School of Biology, Calgary, Calgary, Alberta, Canada Dr Claire Anderson The Australian National University, Division of Plant Science, Research Canberra, ACT Dr Thaddeus C George School of Biology, The Australian National DVS Sciences, Sunnyvale, CA, US University, Canberra, ACT Dr Rafael Casellas Genomics and Immunity Section and Dr Bronwyn Gunn Professor Kaarin Anstey Laboratory of Molecular Immunogenetics, Department of Microbiology and Centre for Research on Ageing, Health The National Institute of Arthritis and Immunology, University of North Carolina and Wellbeing, Research School of Musculoskeletal and Skin Diseases, School of Medicine, Chapel Hill, NC, USA Population Health, The Australian National National Institutes of Health, Bethesda, University, Canberra, ACT MD, USA and Center for Cancer Professor Glenda Halliday Research, National Cancer Institute, Neuroscience Research Australia, Sydney, Dr Tal Arnon Bethesda, MD, USA NSW Department of Microbiology & Immunology, University of California, San Professor Rhodri Ceredig Professor Douglas Hanahan Francisco, CA, USA Regenerative Medicine Institute, National Swiss Institute for Experimental Cancer University of Ireland, Galway, Ireland Research, Lausanne, Switzerland and Assistant Professor Regan Ashby School of Life Sciences, The Swiss The Faculty of Education, Science, Professor Macdonald Christie Federal Institute of Technology, Lausanne, Technology and Mathematics, University Pharmacology, School of Medical Switzerland of Canberra, Bruce, ACT Sciences, Sydney Medical School, The University of Sydney, Sydney, NSW Professor Philip Hogg Professor Emily Banks Lowy Cancer Research Centre, Sydney, National Centre for Epidemiology and Dr Rebecca Coll NSW Population Health, The Australian National School of Biochemistry & Immunology, University, Canberra, ACT Trinity Biomedical Sciences Institute, Dr Jeff Holst Dublin, Ireland Centenary Institute of Cancer Medicine Professor Mark Bellgrove and Cell Biology, Camperdown, NSW School of Psychology and Psychiatry, Dr Lyndsey Collins-Praino Monash University, Clayton, VIC Taub Institute for Research on Alzheimer’s Professor Marc Jenkins Disease and the Aging Brian, Columbia Department of Microbiology, University of Dr Rachel Bashford-Rogers University Medical Center, New York, NY, Minnesota, Minneapolis, MN, USA Wellcome Trust Sanger Institute, USA Cambridge, UK Dr Victoria Johnston Professor Michael Cowley Department of Physiology, Monash Dr Purmina Bhat Department of Physiology, Monash University, Clayton, VIC University of Queensland Diamantina University, Clayton, VIC Institute, Brisbane, QLD Dr Axel Kallies Dr Amanda Craig Division of Molecular Immunology, Dr Brian Billups Translational Neuroscience Facility and Walter and Eliza Hall Institute of Medical Department of Pharmacology, University Department of Physiology, University of Research, Melbourne, VIC of Cambridge, Cambridge, UK New South Wales, Randwick, NSW Professor Moustapha Kassem Professor Roland Brandt Mr Alex Delbridge Laboratory of Molecular Endocrinology, Department of Neurobiology, University of Molecular Genetics of Cancer Division, Medical Biotechnology Centre, University Osnabrück, Osnabrück, Germany The Walter and Eliza Hall Institute of of Southern Denmark, Odense, Denmark Medical Research, Melbourne, VIC Assistant Professor Timothy Professor Rajiv Khanna Bredy Professor Geoff Farrell Australian Centre for Vaccine Queensland Brain Institute, The University ACT Health, ANU Medical School and Development, Queensland Institute of of Queensland, Brisbane, QLD Liver Research Unit, Canberra Hospital, Medical Research, Brisbane, QLD Canberra, ACT Dr Anne Bruestle Campbell Family Cancer Research Professor Mark A Febbraio Institute, Toronto, ON, Canada Cellular and Molecular Metabolism Laboratory, Baker IDI Heart & Diabetes Institute, Melbourne, VIC
72 The John Curtin School of Medical Research Assistant Professor Evelyn Lambe Dr James Murphy Dr Nikolay Shirokikh Department of Physiology, University of The Walter and Eliza Hall Institute of Moscow Regional State Institute of Toronto, Toronto, ON, Canada Medical Research, Melbourne, VIC and Humanities and Social Studies, Ministry of Department of Medical Biology, University Education of Moscow Region, Kolomna, Associate Professor of Melbourne, Melbourne, VIC Moscow Region, Russia Mong-Hong Lee Professor Daniel Osorio Dr Avinash R Shenoy Department of Molecular and Cellular Oncology, The University of Texas MD School of Life Sciences, University of Faculty of Medicine, Department of Anderson Cancer Center and The Sussex, Sussex, UK Medicine, Imperial College, London, UK University of Texas Graduate School of Biomedical Sciences, Houston, TX, USA Dr Justin O’Sullivan Professor Rob Shepherd The Liggin Institute, University of Bionics Institute, Melbourne, VIC Professor Gordon Lynch Auckland, Auckland, New Zealand Department of Physiology, The University Associate Professor Barry of Melbourne, Melbourne, VIC Dr Weiming Ouyang Slobedman US Food and Drug Administration, USA Cytomegalovirus Research Group, Professor Vaughan Macefield Westmead Millennium Institute of Medical Integrative Physiology, School of Professor Ed Palmer Research, Sydney, NSW Medicine, University of Western Sydney, Laboratory of Transplantation Immunology Campbelltown, NSW & Nephrology, The Basel Institute for Dr Kosuke Takemura Immunology Basel, Switzerland Department of Human Pathology, Tokyo Professor Charles Mackay Medical and Dental University, Tokyo, Department of Immunology, Monash Dr Jose Polo Japan University, Clayton, VIC Reprogramming and Epigenetics Laboratory, Monash University, Clayton, VIC Professor Patrick Tam Associate Professor Alexander Embryology Research Unit, Children’s Maier Dr Satyajit Rath Medical Research Institute, Westmead, Biomedical Sciences and Biochemistry, National Institute of Immunology, Sydney, NSW Research School of Biology, The New Delhi, India Australian National University, Canberra, Professor Narci Teoh Professor Linda Richards ACT School of Clinical Medicine, ANU Medical Queensland Brain Institute, The University School and Gastroenterology and Dr Judith Mandl of Queensland, Brisbane, QLD Hepatology Research Unit, Canberra Lymphocyte Biology Section, Laboratory Hospital, Canberra, ACT Dr Alice Richardson of Systems Biology, National Institute of Allergy and Infectious Diseases, National Mathematics & Statistics Academic Associate Professor Michael Institutes of Health, Bethesda, MD, USA Program, The University of Canberra, Valenzuela Bruce, ACT Regenerative Neuroscience Group, Brain Professor Sonya Marshall- & Mind Research Institute, University of Dr Samantha Salvage Gradisnik Sydney, Sydney, NSW National Centre for Neuroimmunology Department of Physiology, Development and Emerging Diseases, Griffith Health and Neuroscience, University of Scientia Professor Fred Institute, Griffith University, Gold Coast, Cambridge, Cambridge, UK Westbrook QLD Professor Tim Senden School of Psychology, University of New South Wales, Randwick, NSW Professor David McConkey Department of Applied Mathematics, Department of Cancer Biology and Research School of Physics and Dr Murray Whitelaw Engineering, The Australian National Department of Urology, University of School of Molecular and Biomedical University, Canberra, ACT Texas MD Anderson Cancer Center, Science, University of Adelaide, Adelaide, Houston, TX, USA Professor Frances Separovic SA Professor Eileen McLaughlin School of Chemistry, Bio21 Institute, The Dr Vihandha Wickramasinghe University of Melbourne, Melbourne, VIC School of Environmental and Life MRC Cancer Unit, University of Sciences, The University of Newcastle, Dr R Shankar Cambridge, Cambridge, UK Newcastle, NSW CSIR Institute of Himalayan Resource Technology, Palampur, India
Annual Review 2013 73 COLLABORATIONS
Dr T Dan Andrews Associate Professor Associate Professor Professor N Hayward Queensland Mauricio Arcos-Burgos John M Bekkers Institute of Medical Research, Brisbane, Genetics of ADHD and disruptive Excitability and hyperexcitability of neural QLD Professor G Mann Westmead behaviours circuits in the rodent piriform cortex Hospital, Sydney, NSW BioPlatforms Australia, Sydney, NSW Dr M Muenke National Human Genome Professor S Nelson Brandeis Research Institute, National Institutes of University, Waltham, MA, US Professor Health, Bethesda, MD, USA G Augustine National University of Dr T Dan Andrews and Dr M Casas, Dr J-A Ramos-Quiroga Singapore, Singapore and Korea Institute Professor Chris C Goodnow and Dr M Ribases Vall d’Hebron of Science and Technology, Seoul, Republic of Korea Genetic determinants of clinical response Institut de Recerca (VHIR) through the to TNF antagonist drugs in rheumatoid International Multicentre Persistent ADHD Neural circuits for odour-processing in the arthritis Collaboration Consortium (IMPACT), rodent piriform cortex ‘in vivo’ Barcelona, Spain Dr C Perera, Canberra Hospital, Professor M Larkum Charité‑Humboldt Canberra, ACT Dr MT Acosta Children’s National University, Neuroscience Research Hospital, Children’s Research Institute Center, Berlin, Germany (CRI), Washington, DC, USA Ms Nurdiana Anuar, Persistent firing in cortical interneurons: Genetics of autoimmune disorders Mechanisms and potential anticonvulsant Professor David Tremethick Dr J-M Anaya Centre for Autoimmune role and Dr Tatiana A Soboleva Diseases Research, University of Rosario, Dr M McDonnell Institute of An investigation on the role of histone Bogota, Colombia Telecommunications Research, University variant H2A.Lap1 Genetics of ADHD of South Australia, Adelaide, SA Professor P Koopman, Dr J Bowles and Dr M Bellgrove School of Psychological Test of novel anticonvulsants for clinical Ms T-L Davidson Institute for Molecular Sciences, Monash University, Melbourne, applications Bioscience, The University of Queensland, VIC Dr J Daniel Children’s Medical Research Brisbane, QLD Dr PD Gregory and Dr Genetics of Major Depressive Disorder Institute, University of Sydney, Sydney, L Zhang Sangamo BioSciences Inc., and genetics of Morbid Obesity NSW Richmond, CA, USA Dr R Arkell Research School of Biology, The Australian National Professor J Licinio and Professor M-L University, Canberra, ACT Wong Translational Medicine, Flinders Dr Andrew Bell University, Adelaide, SA and South Studies of otoacoustic emissions from the Australian Health and Medical Research human cochlea Dr Ehsan Arabzadeh Institute, Adelaide, SA Dr W Jedrzejczak Institute of Physiology Whisker sensory system, from receptor to Genetics of ADHD and substance use and Pathology of Hearing, Warsaw, behaviour disorders Poland Professor M Diamond International Dr E Proal Neuroingenia, Mexico City, School for Advanced Studies, Trieste, Italy Mexico Dr Edward M Bertram Informational basis of sensory adaptation Genetics of substance use disorders Role of TNF family member LIGHT in EAE Professor C Clifford Department of Dr J DeLeon Department of Psychiatry, Dr M Staykova, Dr D Linares and S Psychology, University of Sydney, Sydney, University of Kentucky, Lexington, KY, Fordham Neurosciences Research Unit, NSW USA The Canberra Hospital, Canberra, ACT Neuronal and behavioural correlates of Genetics of ADHD Dr K Pfeffer and Dr S Scheu Institute choice Dr F Levy School of Psychiatry, University of Medical Microbiology and Hospital Professor F Westbrook Department of New South Wales, Randwick, NSW Hygiene, University of Dusseldorf, of Psychology, University of New South Dusseldorf, Germany Wales, Sydney, NSW Genetics of Alzheimer’s Disease and mild cognitive impartment, and genetics Identifying genes involved in Sensory and The role of context in sensory processing of disphemia Metabolic pathways Dr N Price Department of Physiology, Neurosciences Group University of Professor JK Seong, Seoul National Monash University, Clayton, VIC Antioquia, Medellín, Antioquia, Colombia University, Seoul, Republic of Korea
74 The John Curtin School of Medical Research Dr Edward M Bertram and Genetics Laboratory, Queensland Institute Structure and function of lysine Professor Chris C Goodnow of Medical Research, Brisbane, QLD demethylase enzymes Understanding anthracycline induced Dr S Rao Department of Applied China-Australia Centre for Phenomics cardiotoxicity Science, University of Canberra, Research Dr N Beard, University of Canberra, Canberra, ACT Professor H Tang Centre for Infection Bruce, ACT Effects of drugs that block Vpu ion and Immunity, Chinese Academy channels of Sciences, Institute of Biophysics, China Professor Philip G Board Professor W Fischer School of Biomedical Science and Engineering, Dr Edward M Bertram, Determination of glutathione transferase National Yang‑Ming University, Taipei, Ms Belinda Whittle, structures Taiwan Dr T Dan Andrews and Professor M Parker St Vincent’s Design and development of M2 influenza Medical Research Institute, Melbourne, A inhibitors Mr Matthew Field VIC Professor W Hu Guangzhou Institute Identifying genes involved in EAE and Function of Zeta and Omega class GSTs of Biomedicine and Health, Chinese immunity Professor MW Anders Department of Academy of Sciences, Science Park, Dr J Gommerman and Dr L Osborne Pharmacology and Physiology, University Guangzhou, China University of Toronto, ON, Canada of Rochester Medical Centre, Rochester, Identifying rare disease genes NY, US Mr Aaron Chuah Structure of γ-glutamyl cyclotransferase Dr T Dudding, Dr B Kamien, Dr H Goel Alpine Frog and Bettong Genotyping by and Dr A Ronan Hunter Genetics, Associate Professor A Oakley School Sequencing Newcastle, NSW of Chemistry, University of Wollongong, Wollongong, NSW Dr S Banks Fenner School of Environment and Society, The Australian Dr Anneke C Blackburn The expression of γ-glutamyl National University, Canberra, ACT cyclotransferase in human cancer Mouse mammary tumour susceptibility loci Genotyping by Sequencing Professor Y Eishi and Dr K Takemura Professor DJ Jerry Veterinary Department of Human Pathology, Tokyo Dr J Borevitz Research School of and Animal Sciences, University of Medical and Dental University, Tokyo, Biology, The Australian National University, Massachusetts, Amherst, MA, US Japan Canberra, ACT Targeting breast cancer with The role of Omega class GSTs in TLR4 Tomato RNA-seq Differential Expression dichloroacetate and the mitochondrial signalling Analysis toxin PENAO Professor L Neill and Dr R Coll School Dr A-M Catanzariti Research School of Professor PJ Hogg and Dr PJ Dilda of Biochemistry and Immunology, Trinity Biology, The Australian National University, Lowy Cancer Research Centre, University Biomedical Sciences Institute, Trinity Canberra, ACT of New South Wales, Sydney, NSW College, Dublin, Ireland Arabidopsis RNA-Seq Differential Targeting of Two Aspects of Metabolism Expression and Degradome Investigation (TOTAM) for Cancer Therapy Dr Marco G Casarotto Dr C Helliwell CSIRO Plant Industry, Professor JE Dahlstrom Anatomical Canberra, ACT Pathology, ACT Pathology, Canberra Molecular recognition of the ryanodine receptor Genotyping by Sequencing Australian Hospital and ANU Medical School, The Native Plants Australian National University, Canberra, Dr M Samso Department of Physiology ACT and Associate Professor D Yip and Biophysics, VCU School of Medicine, Dr E James Royal Botanic Gardens, Medical Oncology, Canberra Hospital, Richmond, VA, US Melbourne, VIC Canberra, ACT and ANU Medical School, Determination of glutathione transferase Abalone and Alpine Plant Genotyping By The Australian National University, structures Sequencing Canberra, ACT Professor M Parker Biota Structural Dr A Miller Departments of Zoology Human breast cancer modifier gene Biology Laboratory, St Vincent’s Institute, and Genetics, Faculty of Science, The discovery through the Kathleen Cuningham Melbourne, VIC University of Melbourne, Melbourne, VIC Foundation Consortium for Research into Transcriptome sequencing and differential Familial Breast Cancer (kConFab) Interactions between the alpha and beta subunits of the dihydropyridine receptor expression analysis of four Arabidopsis Dr A Spurdle The Molecular Cancer cultivars Epidemiology Laboratory, Queensland Associate Professor A Oakley Dr Iain Searle School of Molecular Institute of Medical Research, Brisbane, Department of Chemistry, University of and Biomedical Science, University of QLD Dr G Chenevix-Trench Cancer Wollongong, Wollongong, NSW Adelaide, SA
Annual Review 2013 75 Mr Aaron Chuah and Genomic medicine for human immune Concord, NSW Dr D Fulcher Professor Trevor Lamb deficiency Department of Immunopathology, Dr D Tscharke Research School of The Children’s Hospital at Westmead, TriPyGDU: Transcriptome-RNAseq Biology, The Australian National University, Westmead, NSW Interactive Python Graphical Data Unifier Canberra, ACT Dr D Fulcher Department Dr D Hunt Lions Eye Institute & University of Immunopathology, The Children’s Dr Lucy Coupland of Western Australia Oceans Institute, The Hospital at Westmead, Westmead, NSW Mechanisms and novel treatment University of Western Australia, Perth, WA A randomised trial to assess antibody and avenues for auto-immune cytopenias Associate Professor Ian cellular responses to hepatitis B vaccine in patients with chronic kidney disease Dr J D’Rozario and Dr P Crispin Cockburn (VaccineCKD) Haematology Department, Canberra Hospital, Canberra, ACT Imaging the CD8+ T cell response to Dr K Karp Canberra Hospital, Canberra, malaria parasites in the liver ACT Characterisation of erythrocyte abnormalities in hemoxygenase-1 Dr R Amino and Dr R Menard Institut CCR5 expression on B cells in immune deficient mice Pasteur, Paris, France Assistant deficiency Professor V Ganusov Theoretical Professor R Stocker, Dr R Winter Professor H Korner University of Immunology Laboratory, University of and Dr S Fraser Sydney Medical School, Tasmania, Hobart, TAS Tennessee, Knoxville, TN, USA The University of Sydney, Sydney, NSW STAT3 and hyper IgE syndrome Understanding B cell responses to malaria vaccine antigens at the single cell Dr S Tangye Garvan Institute of Medical Dr Stephen Daley level Research, Sydney, NSW Pulmonary hypertension caused by a Dr R Seder Vaccine Research Centre, novel ENU-induced variant of Tomm40 National Institute of Allergy and Infectious Professor Matthew C Cook Professor L Arnolda The Canberra Diseases, National Institutes of Health, and Dr Stephen Daley Hospital, Canberra, ACT and ANU Bethesda, MD, USA Dr Francis Ndungu Medical School, The Australian National Kenya Medical Research Institute, KEMRI- Immune response after adult thymectomy University, Canberra, ACT Wellcome Trust Research Programme, Kilifi, Professor G Chong Canberra Hospital, Kenya Professor Kevin Marsh Nuffield Canberra, ACT Department of Medicine, University of Dr Vincent Daria Oxford, Oxford, UK Professor Matthew C Cook Adaptive wavefront correction of efficient delivery of light through brain tissues and Professor Chris C Professor Matthew C Cook Dr S Lee Research School of Australian and New Zealand Antibody Goodnow Engineering, The Australian National Deficiency Allele (ANZADA) study Cell of origin of B cell Non-Hodgkin’s University, Canberra, ACT Dr S Adelstein and Dr R Garsia lymphoma Synthesising photo-switchable Department of Immunology, Royal Prince Dr D Talaulikar Canberra Hospital, nanoparticle agents for cell imaging and Alfred Hospital, Camperdown, NSW Dr Canberra, ACT analysis T DeMalmanche HAPS Immunology, Dr A Tricoli Research School of Newcastle, NSW Dr P Hissaria Royal Professor Matthew C Cook, Engineering, The Australian National Adelaide Hospital, Adelaide, SA Dr University, Canberra, ACT M French School of Pathology and Professor Chris C Goodnow Analysing Soft-matter interactions using Laboratory Medicine, The University of and Professor Carola G Western Australia, Perth, WA Dr S Choo multiple beam optical tweezers Royal Melbourne Children’s Hospital, Vinuesa Professor E Sevick Research School Melbourne, VIC Professor D Campbell Australian point mutation in systemic of Chemistry, The Australian National and Dr S Mehr The Children’s Hospital lupus erythematosus (APOSLE) study University, Canberra, ACT at Westmead, Westmead, NSW Dr S Riminton Department of Immunology, Professor P Gatenby Canberra Concord Repatriation General Hospital, Hospital, Canberra, ACT Dr S Adelstein Concord, NSW Dr D Fulcher and Dr R Garsia Department of Department of Immunopathology, Immunology, Royal Prince Alfred Hospital, The Children’s Hospital at Westmead, Camperdown, NSW Dr D Mallon Westmead, NSW Fremantle Hospital, Fremantle, WA Dr S Alexander The Children’s Hospital at Westmead, Westmead, NSW Dr S Riminton Department of Immunology, Concord Repatriation General Hospital,
76 The John Curtin School of Medical Research Professor Angela Dulhunty Single channel activity of RyRs from Dr Julia Ellyard and normal human and DM1 muscle Ryanodine receptor regulation in health Professor Carola Vinuesa and disease Professor K North and Dr N Clarke Discipline of Paediatrics and Child Health, Loss-of-fuctional variants in AIRE Dr NA Beard Centre for Research in The Children’s Hospital at Westmead, The Professor P Peterson and Dr M Therapeutic Solutions, University of University of Sydney, Sydney, NSW Saare Institute of Molecular and Cell Canberra, ACT New cardiac ryanodine receptor inhibitors Biology, University of Tartu, Estonia Various aspects of ryanodine receptor for the treatment of heart failure IFN-alpha secretion in SLE physiology Professor L Arnolda Canberra Hospital, Professor P Hertzog Centre for Innate Dr D Laver Hunter Medical Research Canberra, ACT and ANU Medical School, Immunity and Infectious Diseases, Institute, University of Newcastle, The Australian National University, Monash Institute of Medical Research, Newcastle, NSW Canberra, ACT Melbourne VIC Contribution of splicing defects and Mutations in CLIC-2 proteins leading to mutations on ryanodine receptors ryanodine receptor channelopathy heart Dr Anselm Enders function failure and intellectual deficit Professor RT Dirksen and Dr L Wei Analysis of the putative phospholipid Professor C Schwartz and Dr E Axelov transporter ATP11C Department of Pharmacology and JC Self Research Institute of Human Physiology, University of Rochester Genetics, Greenwood Genetic Center, Professor S Broer Research School Medical Centre, Rochester, NY, USA Greenwood, SC, USA and Department of Biology, The Australian National Actions of triadin and calsequestrin on of Physics, Computational Biophysics University,Canberra, ACT excitation-contraction coupling and Bioinformatics, Clemson University, Analysis of liver tumor development in Clemson, SC, USA Professor RT Dirksen and Dr L Wei ATP11C deficient mice Department of Pharmacology and Actions of ϕ-LITX-Lw1a on ryanodine Professor N Teoh Canberra Hospital, Physiology, University of Rochester receptors Canberra, ACT Medical Centre, Rochester, NY, USA Dr J Smith and Professor P Alewood Analysis of NKT development and CPVT mutations in the cardiac RyR Institute for Molecular Biosciences, The function in mouse strains with ENU University of Queensland, St Lucia, QLD Professor M Varsanyi Institut für induced point mutations Physiologische Chemie, Ruhr Universität, The roles of calsequestrin in fast and slow Professor D Godfrey Department of Bochum, Germany twitch skeletal muscle Microbiology and Immunology, University FRET analysis of DHPR beta subunit Professor F Protasi CeSI, Center for of Melbourne, Melbourne, VIC binding to the skeletal RyR Research on Ageing, Department of Analysis of MHC invariant chain Neuroscience and Imaging, Università Professor B Fruen Biochemistry, processing and development of dendritic Gabriele d’Annunzio, Chieti, Italy Molecular Biology and Biophysics, cells in SPPL2A deficient mice University of Minnesota, Minneapolis, MN, Effects of aging on the distribution of Professor J Villadangos Department of USA excitation-contraction coupling proteins in Microbiology and Immunology, University human skeletal muscle Accessory protein binding sites on the 3D of Melbourne, Melbourne, VIC profile of RyR1 and RyR2 Professor Paul Smith Trauma and Genetic analysis of humans with Orthopaedic Research Unit, Canberra Professor T Wagenknecht and Dr Z immunodeficiency of unknown aetiology Hospital, Canberra, ACT Liu Wadsworth Center, New York State Professor H Eibel and Professor S Ehl Department of Health, Albany, NY, USA Effects of the beta subunit of the DHPR Centre for Chronic Immunodeficiency, on excitation-contraction coupling The 3D location of the binding domains Freiburg, Germany, Professor K in RyR1 Professor M Schneider Biochemistry Schwarz University of Ulm, Ulm, and Molecular Biology, University of Germany Assistant Professor M Samso Maryland School of Medicine, Baltimore, Department of Physiology and MD, USA Biophysics, Virginia Commonwealth University, Richmond, VA, USA Abnormalities in ryanodine receptor activity in human heart failure Professor C dos Remedios Department of Anatomy, The University of Sydney, Sydney, NSW
Annual Review 2013 77 Dr Anselm Enders and Professor Chris C Goodnow, Professor N Dear Research and Biomedical Services, South Australian Dr Edward M Bertram Dr Edward M Bertram, Health and Medical Research Institute, Broad based phenotyping of genes that Dr Katrina Randall and Dr Adelaide, SA affect the Immune system Stephen Daley Australian Phenomics Network Project: Australian Phenome Bank Professor M Hrabe De Angelis, Dr H Identifying genes for immunity and Cryopreservation Fuchs and Dr V Gailus-Durner and the tolerance members of the German Mouse Clinic, Dr P Sharp Animal Resources Centre, Dr J Cyster, Professor L Lanier, Helmholtz Zentrum, Munich, Germany Murdoch University, Canningvale, WA Professor A Weiss and Dr Jeroen Roose University of California, San Australian Phenomics Network Project: Professor Chris C Goodnow Francisco, CA, USA RNAi technologies Human antibody repertoires Associate Professor R Teasdale Dr D Dunn-Walters King’s College, Professor Chris C Goodnow Molecular Cell Biology Division, Institute London, UK Professor D Kipling Cardiff and Professor Matthew C for Molecular Bioscience, Brisbane, University, Cardiff, UK QLD Professor R Johnstone Gene Cook Regulation Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC Dr L Professor Chris C Goodnow Ndfip1 aborts tolerogen-induced division and differentiation of CD4+ T Winteringham Leukaemia Research and Dr Edward M Bertram cells to prevent Th2 inflammation and Group, The Harry Perkins Institute autoimmunity of Medical Research, Nedlands, WA Role of hnRNPLL in generating protective Dr R Dickins Molecular Medicine immunity to mycobacteria tuberculosis Professor S Tan and Dr J Howitt Florey Division, Walter and Eliza Hall Institute Dr B Saunders and Professor W Neurosciences Institute, The University of of Medical Research, Melbourne, VIC Britton Mycobacterial Research Group, Melbourne, Melbourne, VIC Dr S Kumar Centre for Cancer Biology, SA Pathology, Centenary Institute of Cancer Medicine Professor Chris C Goodnow and Cell Biology, Sydney, NSW Adelaide, SA Dr L Wu Department of Immunology, Genentech, San Francisco, and Dr Anselm Enders Mutagenetix – A database of mutations CA, USA and phenotypes induced by ENU NKT cell mutations Professor B Beutler University of Texas Professor Chris C Goodnow Professor D Godfrey Department of Southwestern Medical Center, Dallas, TX, Immunology, The University of Melbourne, USA and Dr Michael Dobbie Melbourne, VIC International Mouse Phenotyping Australian Phenomics Network Project: Consortium ES cell to Mouse Service Professor Chris C Goodnow, Professor S Brown MRC Harwell, Associate Professor I Smyth Dr Keiskuke Horikawa, Oxford, UK Department of Biochemistry & Molecular Mr Yogesh Jeelall and Biology Faculty of Medicine, Nursing Associate Professor Professor Chris C Goodnow, & Health Sciences, Monash University, Clayton, VIC Matthew C Cook Dr Edward M Bertram, Australian Phenomics Network Project: Human lymphoma mutations Associate Professor Guna ENU Variant Collection Dr D Talaulikar Canberra Hospital, Karupiah, Dr Anselm Enders Professor B Kile Chemical Biology Canberra, ACT and Dr Katrina Randall Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC Systems approach to Inflammation and Professor Chris C Goodnow Professor S Foote Australian School Immunity of Advanced Medicine, Faculty of Human and Dr Ian Parish Professor R Ulevitch, Dr L Teyton The Sciences, Macquarie University, Imaging proximal T cell signalling Scripps Research Institute, La Jolla, CA, Sydney, NSW USA Professor B Beutler University Professor K Gaus Centre for Australian Phenomics Network Project: of Texas Southwestern Medical Center, Vascular Research, University of New Mouse Pathology Dallas, Texas, USA Dr A Aderem and South Wales, Sydney, NSW Dr I Shmulevic Institute for Systems Professor John Furness Autonomic Biology, Seattle, WA, USA Dr G Nolan and Sensory Neuroscience Laboratory, Department of Microbiology and University of Melbourne, Melbourne, VIC Immunology, Stanford University, Palo Alto, CA, USA
78 The John Curtin School of Medical Research Professor Chris C Goodnow Professor Jill E Gready Modulation of hemi channel function by mutations in connexin40 and Professor Carola G Characterization of photosynthetic Vinuesa efficiency and nutritional quality of the Professor K Machaca Department of “Inca” superfoods, potato and quinoa, of Physiology and Biophysics, Weill Cornell Immunity and Infection Genomics Andean for breeding improved cultivars with Medical College‑Qatar, Education City, Consortium increase productivity and nutritional benefits Doha, Qatar Professor S Broer Research School of Biology, The Australian National Dr R Cornall Nuffield Department of Dr C Rodriguez Universidad Técnica de University, Canberra, ACT Clinical Medicine, Oxford University, Ambato, Ambato, Ecuador Oxford, UK Professor J Bell The Modulation of gap junctional coupling in Weatherall Institute of Molecular Development of ANU technology for the oocytes by mutations in connexin40 Medicine, Oxford University, Oxford, design of variant Rubisco proteins with improved catalytic activity Professor BJ Nicholson Department of UK Professor W Britton and Dr B Biochemistry, University of Texas Health Saunders Centenary Institute of Cancer Professor JE Evans Research School of Sciences Center, San Antonio, TX, US Medicine and Cell Biology, The University Biology, The Australian National University, of Sydney, Sydney, NSW Professor M Canberra, ACT Dr J van Rie and Dr A Lathrop National Centre for Genotyping, Gallé Bayer Bioscience, Ghent, Belgium Associate Professor Evry, France Gavin Huttley Dr Rebecca Haddock Reconstruction of coral phylogeny Professor Chris C Goodnow, Role of NOX-2 in obesity-related Dr S Forêt Research School of Biological Professor Carola G Vinuesa hypertension Sciences, The Australian National and Professor Matthew C Associate Professor GG Drummond University, Canberra, ACT Cook Department of Pharmacology, Monash Mathematical models of sequence University, Melbourne, VIC divergence Molecular and cellular studies of the adaptive immune response in health and Dr C Burden Mathematical Sciences disease Professor Caryl E Hill Institute, The Australian National University, Canberra, ACT Professor C Mackay and Professor Subcellular localisation of voltage F Mackay-Fisson, Monash University, dependent calcium channels and gap Annotation of novel genomes; and, Clayton, VIC Professor J Sprent, junctions in the vasculature identifying genes undergoing natural selection Emeritus Professor A Basten, Dr S Dr SL Sandow Department of Tangye, Dr R Brink and Dr Daniel Pharmacology, School of Medical Dr A Papanicolaou CSIRO Ecosystem Christ Garvan Institute of Medical Sciences, The University of New South Sciences, Canberra, ACT Research, Sydney, NSW Wales, Sydney, NSW Development of a software library for Blood pressure recordings in freely genomic biology; numerous other Professor Chris C Goodnow moving animals projects related to the evolutionary dynamics of sequence divergence and Ms Belinda Whittle Dr R Brown Department of Physiology, Professor R Knight Department of Melanoma Project and SNV Detection Monash University, Melbourne, VIC Chemistry and Biochemistry, University of Mr C Broadley Australian Genome Modulation of gap junctional coupling by Colorado, Boulder, CO, USA Research Facility, Westmead, NSW mutations in connexin40 Development of Markov-process models Dr A Ashton Division of Perinatal to measure the influence of sequence- Professor Chris C Goodnow, Research, Kolling Institute of Medical neighbourhoods on mutation dynamics Research, Royal North Shore Hospital, Dr Steve WInslade and University of Sydney, NSW Associate Professor VB Yap Dr T Dan Andrews Department of Statistics and Applied Role of T-type calcium channels in Probability, National University of Framework Data Sets vasoconstriction and blood pressure Singapore, Singapore Mr A Gilbert Bioplatforms Australia, Professor PB Hansen Department of Sydney, NSW Cardiovascular and Renal Research, University of Southern Denmark, Odense, Denmark
Annual Review 2013 79 Associate Professor Gavin Actin-based motility as a virulence of Health Sciences and Medicine, Bond Huttley and Dr Åsa Pérez- mechanism and potential as an antiviral University, Gold Coast, QLD target Viral diseases and genetics of the host Bercoff Dr T Newsome School of Molecular response Understanding the genetic causes of Bioscience, University of Sydney, Sydney, Associate Professor M Heise virulence in fungal pathogens NSW Department of Genetics, University of Professor W Meyer Molecular Mycology Pathophysiological significance of reverse North Carolina, Chapel Hill, NC, USA Research Laboratory, Centre for Infectious signalling through membrane TNF Metabolomic profiles and clinical Disease and Microbiology, Westmead Professor A Alcami Centro de Biología microbiology of ME-CFS Millennium Institute, Westmead Hospital, Molecular Severo Ochoa, Madrid, Spain Sydney Medical School Westmead, The Dr H Butt, Associate Professor P Gooley University of Sydney, Westmead, NSW Associate Professor and C Armstrong Bio21 Institute and University of Melbourne, Melbourne, VIC Professor H Nevalainen and Professor Guna Karupiah, Dr Geeta I Paulsen Department of Chemistry Knowledge discovery in pathology and Biomolecular Sciences, Macquarie Chaudhri and Professor databases for enhanced laboratory University, Sydney, NSW Chris C Goodnow diagnosis Systems approach to immunity and G Koerbin ACT Pathology, Canberra Dr Andrew James inflammation Hospital, Canberra, ACT MRI and source modelling Professor R Ulevitch The Scripps Immunology of ME-CFS Research Institute, La Jolla, CA, USA Dr S Inverso Pasteur Institute, Paris, Professor S Marshall Griffith Health Professor B Beutler University of France Institute, Griffith University, Gold Coast, Texas Southwestern Medical Center, QLD Modelling of cochlear mechanics Dallas, Texas, USA Professor A Biomedical and clinical study of ME-CFS Professor HP Wit University of Aderem Seattle Biomedical Research patients Groningen, Groningen, The Netherlands Institute, Seattle, WA, USA Professor G Nolan Department of Microbiology and Dr D Lewis CFS Discovery, Donvale Immunology, Stanford University, Palo Specialist Medical Centre, Donvale, VIC Dr Ben Kaehler Alto, CA, USA Genetic distance for a general non- Professor Ted Maddess stationary Markov Substitution Process Professor Trevor D Lamb Multifocal visual evoked potentials Associate Professor VB Yap Evolution of the vertebrate eye Department of Statistics and Applied Dr SN Abdullah Raja Isteri Pengiran Probability, National University of Professor SP Collin, Professor D Anak Saleha (RIPAS) Hospital, Bandar Singapore, SIngapore Hunt and Associate Professor N Hart Seri Begawan, Brunei School of Animal Biology, University of Sources of triphasic waves in hepatic Lessons from Nature Western Australia, Perth, WA encephalopathy Dr H Trueman CSIRO Ecosystem Recovery of human cone photoreceptors Dr I Al Homoud King Faisal Specialist Sciences, Canberra, ACT following bleaching Hospital and Research Centre, Saudi Dr O Mahroo Department of Physiology, Arabia Associate Professor Guna Development and Neuroscience, Polarisation sensitivity in squid eyes Karupiah and Dr Geeta University of Cambridge, Cambridge, UK Professor Y Nagai Kokushikan Chaudhri University, Tokyo, Japan Associate Professor Brett Modulation of the immune response by Multifocal visual evoked potentials and virus-encoded cytokine homologs Lidbury visual attention in multifocal pupillography Professor M Buller St Louis University, Systematic review and meta-analyses as Associate Professor Y Rosli Universiti St Louis, MO, USA Professor A Alcami a complementary technique to enhance Kebangsaan Malaysia, Bangi Selangor, Centro de Biología Molecular Severo predictions from pattern recognition Malaysia Ochoa, Madrid, Spain involved in a novel animal replacement Causes of glaucoma Induction of long-lived antiviral humoral system Professor M Ritkes-Hoitinga immunity and Dr K Wever SYRCLE, Radboud Professor W E Sponsel Department University Nijmegen, The Netherlands of Biomedical Engineering, San Antonio, Dr T Newsome School of Molecular Pattern recognition in pathology data TX, USA Professor JD Victor Brain and Bioscience, University of Sydney, Sydney, Mind Research Institute and Department NSW Professor R Brink Garvan Institute and in vitro validation of diagnostic data networks of Neurology, Cornell Weill Medical for Medical Research, Sydney, NSW College, New York, NY, USA Associate Professor T Badrick Faculty
80 The John Curtin School of Medical Research Diabetic retinopathy and macular Development of a liposome-based TB Dr Hardip Patel, Dr degeneration vaccine Gilberto Paz-Filho, Claudio Professor C Nolan and Dr RW Essex Associate Professor J Triccas ANU Medical School, The Australian Department of Infectious Diseases Mastronardi, Mr Angad National University, Canberra, ACT and Immunology, University of Sydney, Johar, Mr Aaron Chuah, Sydney, NSW Associate Professor Gavin Dr Claudio A Mastronardi Heparan sulfate and Alzheimer’s disease Huttley and Professor Translational studies in obesity Professor D Small Menzies Research Mauricio Arcos-Burgos Institute, University of Tasmania, Hobart, TAS Professor S Bornstein and Dr M Whole exome sequencing of Ehrhart – Bornstein University of Novel angiogenesis inhibitors extreme morbid obese patients: Dresden, Dresden, Germany Dr P Karuso Department of Chemistry Translational implications for obesity and Translational studies in bone metabolism and Biomolecular Sciences, Macquarie related disorders University, Sydney, NSW Dr M Kelso Professor Rachel Li Trauma and Professor M Boguszewski and School of Chemistry, University of Professor C Boguszewski Universidade Orthopedic Research Laboratory, Wollongong, NSW ANU College of Medicine, Biology and Federal do Paraná, Department of Environment, The Australian National Islet heparan sulfate and type I diabetes Pediatrics, Brazil Professor M-L Wong and Professor J Licinio South Australian University, Canberra, ACT Professor R Rodgers Department of Health and Medical Research Institute, Obstetrics and Gynaecology, University of Adelaide, SA Dr Maxim Nekrasov Adelaide, Adelaide, SA Professor T Kay St Vincent’s Institute, Melbourne, VIC CINDy – Chromosome Interaction Targeted mistletoe tumour therapy Dr Gilberto Paz-Filho Network DYnamics in adipose stem cells Associate Professor L Simson Congenital leptin deficiency Professor P Collas Department of Faculty of Applied Science, University of Biochemistry, Institute of Basic Medical Dr T Delibasi Department of Canberra, Bruce, ACT Sciences, University of Oslo, Oslo, Endocrinology, Ankara Hospital, Ankara, Norway Development of heparan sulfate mimetics Turkey for type 1 diabetes treatment The role of ?? Histone variants Clinical Endocrinology: Multinodular in formation of fear memory Professor M von Itzstein Institute for goitre, metabolic syndrome, obesity and Glycomics, Griffith University, Gold Coast, thyroid disorders Dr T Bredy Queensland Brain Institute, QLD The University of Queensland, Brisbane, Professor H Graf, Professor C QLD Boguszewski, Professor GA de Dr Hardip Patel Carvalho and Dr V Borba Department of Endocrinology of the Federal University Professor Effect of placental gene expression on of Parana, Curitiba, Brazil cord blood during asthma Christopher Parish Translational studies in obesity Associate Professor V Clifton Role of platelets in tumour metastasis Professor S Bornstein and Dr M Robinson Research Institute, University of Ehrhart – Bornstein University of Professor B Chong School of Adelaide, Adelaide, SA Pathology, The University of New South Dresden, Dresden, Germany Physical mapping and assembly of the Wales, Sydney, NSW Pogona vitticeps genome Studies of immune thrombocytopenia Dr Jason Potas Professor A Georges and Associate Professor B Chong School of Professor J Deakin Institute for Applied 670 nm light reduces allodynia following Pathology, The University of New Ecology, University of Canberra, Bruce, spinal cord injury South Wales, Sydney, NSW Professor ACT Associate Professor L Henderson L Khachigian Centre for Vascular Dosage compensation of sex Anatomy & Histology, School of Medical Research, The University of New South chromosomes in snakes Sciences, The University of Sydney, Wales, Sydney, NSW Sydney, NSW Dr P Waters Research School of Biology, Nuclear localisation of heparanase and The Australian National University, The use of nanofibre material for nervous other proteins Canberra, ACT Dr D O’Meally Institute tissue regeneration Professor R Stocker Department of for Applied Ecology, University of Dr D Nisbet Engineering, The Australian Pathology, The University of Sydney, Canberra, Bruce, ACT National University, Canberra, ACT Sydney, NSW Professor L Khachigian Centre for Vascular Research, The University of New South Wales, Sydney, NSW Associate Professor S Rao Faculty of Applied Science, University of Canberra, Bruce, ACT
Annual Review 2013 81 New pharmacological agents that isolate Sequence diversity in the murine Professor Jan Provis individual fibre types in the peripheral nerve cardiomyocyte miRNA population Modelling and managing retinopathy of Professor RT Sudo Universidade Federal Associate Professor D Fatkin and prematurity do Rio de Janeiro, Rio de Janeiro, Brazil Associate Professor C Suter The Victor Chang Cardiac Research Institute, Professor T Chan-Ling University of Sydney, Sydney, NSW Dr Jason Potas, Professor Sydney, NSW Greg Stuart and Professor Exploring the role of microRNA and target processing variability in cardiac Dr Charani Ranasinghe Ted Maddess hypertrophy HIV envelope antibody studies Functional mapping of the dorsal column Professor B Graham The Victor Chang Dr R Center Department of Microbiology nucleus: A new approach to sensory Cardiac Research Institute, Sydney, NSW and Immunology, The University of pathway evaluation following spinal cord Identifying the Targets of miRNA Melbourne, Melbourne, VIC injury Regulation in Cancer HIV-1 Pox-viral vaccine vectors Professor J Morley University of Dr T Beilharz Monash University, Dr D Boyle CSIRO Livestock Industries, Western Sydney, Campbelltown, NSW, Melbourne, VIC Dr S Redmond Graduate School of Australian Animal Health Laboratory, Biomedical Engineering, The University of Yeast hEST1A/B (SMG5/6)-like proteins Geelong, VIC New South Wales, Sydney, NSW contribute to environment-sensing HIV-1 mucosal vaccines and murine adaptive gene expression responses The use of nanofibre material for nervous influenza-HIV-1 studies tissue regeneration Associate Professor J Heierhorst Dr J Stambas Deakin University and St Vincent’s Institute of Medical Research, CSIRO Animal Health Laboratories, Dr D Nisbet Engineering, The Australian Melbourne, VIC National University, Canberra, ACT Geelong, VIC Translational control of the EBV-encoded TNF family members and immune nuclear antigen, EBNA1 Mr Alvin Pratama responses to viral infection and tetramer Dr J Tellam Queensland Institute of studies MicroRNA-146a represses T follicular Medical Research, Brisbane, QLD Dr L Sedger School of Medical and helper cell accumulation Developmental defects of the Molecular Biosciences, The University of Dr R Casellas Genomics and Immunity gastrointestinal tract caused by Technology, Sydney, NSW Section, National Institute of Arthritis dysfunction of a RNA binding protein Mucosal HIV, TB vaccines and CD8 T-cell and Musculoskeletal and Skin Diseases, Professor P Tam Children’s Medical avidity National Institutes of Health, Bethesda, Research Institute, Sydney, NSW MD, USA Dr Andreas Hutloff Chronic Professor A Ramsay Gene Therapy Immune Reactions Group, German Exploring the interface between Program, Louisiana Vaccine Centre, Rheumatism Research Centre Berlin, RNA, enzymes and metabolites in Louisiana State University Health Berlin, Germany cardiomyocytes Sciences Centre, New Orleans, LA, USA Professor M Hentze European Human HIV-specific T cell immunity & T Professor Thomas Preiss Molecular Biology Laboratory (EMBL), cell avidity studies Heidelberg, Germany A sustained dietary change increases Professor A Kelleher Immunovirology epigenetic variation in isogenic mice The role of miRNAs in the generation of and Pathogenesis Program NCHECR, iPS cells The University of New South Wales, Associate Professor C Suter The Sydney NSW and St Vincent’s Centre for Professor A Nagy Lunenfeld- Victor Chang Cardiac Research Institute, Applied Medical Research, Sydney, NSW Sydney, NSW Professor David Martin Tanenbaum Research Institute, Toronto, Children’s Hospital Oakland Research ON, Canada Professor S Grimmond HIV/SIV macaque vaccine studies Institute, Oakland, CA, US Institute for Molecular Bioscience, The Professor S Kent and Dr R De University of Queensland, Brisbane, QLD The prevalence and roles of Rose Department of Microbiology and 5-methylcytosine in eukaryotic Post-transcritional control of gene Immunology, The University of Melbourne, transcriptomes expression in inflammation Melbourne, VIC Associate Professor C Suter The Victor Professor A Ostareck- Mucosal vaccine strategies against Chang Cardiac Research Institute, Sydney, Lederer and PD Dr D Ostareck Chlamydia NSW Professor S Clark and Professor University Hospital Aachen, Professor K Beagley Institute of Health J Mattick The Garvan Institute of Medical RWTH Aachen University, Aachen, and Biomedical Innovation, Queensland Research, Sydney, NSW Germany University of Technology, Brisbane, QLD
82 The John Curtin School of Medical Research Dr Katrina Randall Acetylation of histone H3 at lysine 64 Dr Charmaine J Simeonovic, regulates nucleosome dynamics and DOCK8 in T cells facilitates transcription Professor Christopher R Dr J Oliaro Peter MacCallum Cancer Dr R Schneider Department of Parish and Ms Fui Jiun Centre, Melbourne, VIC Functional Genomics, Institut de Choong Génétique et de Biologie Moléculaire Remodelling of the islet BM after islet et Cellulaire (IGBMC), CNRS UMR, Dr Katrina Randall, Dr isolation and transplantation Strasbourg, France Stephen Daley, Mr Daniel Professor R Rodgers Department Hu and Professor Chris C of Obstetrics and Gynaecology, The Dr Charmaine J Simeonovic Goodnow University of Adelaide, Adelaide, SA Dr Heparan sulfate expression in isolated H Irving-Rodgers School of Medical The Anaef mutation in T cell development human islets and islet transplants Science, Griffith University, QLD Dr J Roose University of California San Professor S Bornstein and Francisco, San Francisco, CA, USA Dr B Ludwig University Clinic Dr Zan-Min Song Carl‑Gustav‑Carus, Technical University, Hirschsprung’s Disease and its Dresden, Germany Dr Danny Rangasamy relationship to immunity Heparan sulfate and heparanase Toward repurposing antiviral drugs in the Associate Professor David Croaker expression in human pancreas treatment of psoriasis Canberra Hospital, Canberra, ACT Clinical Associate Professor JD Professor J-P Molès Institut national Stem cell implantation as a possible future Wilson Department of Endocrinology, de la santé et de la recherche médicale, treatment for Hirschsprung’s disease Canberra Hospital, Canberra, ACT Montpellier, France Professor Heather Young and Exploring the role of L1 retrotransposon Professor John Furness Department of and Rad21 in CRC Cancer Dr Charmaine J Simeonovic, Anatomy and Neuroscience, University of Melbourne, Melbourne, VIC Dr X Huiling Peter MacCallum Cancer Ms S Dhounchak and Centre, Melbourne, VIC Professor Christopher R Prognostic value of LINE-1 Parish Associate Professor retrotransposon expression in breast Loss of islet heparan sulfate in T2D in db/ Christian Stricker cancer db mice Short-term dynamics in small networks Professor JE Dahlstrom Anatomical Professor T Biden and Dr R Laybutt, Pathology, ACT Pathology, Canberra Dr M McDonnell Institute of Garvan Institute of Medical Research, Hospital and ANU Medical School, The Telecommunications Research, University Sydney, NSW Australian National University, Canberra, of South Australia, Adelaide, SA ACT Analysis of epigenetic factors in mouse Dr Charmaine J Simeonovic Professor Greg J Stuart embryonic neural stem cells exposed to and Professor Christopher Action potential initiation and interaction hyperglycemia R Parish with inhibitory synaptic events Professor ST Dheen Department Protection of human islet beta cells by Professor M Häusser The Wolfson of Anatomy, National University of heparin and HS-mimetics Institute for Biomedical Research, Singapore, Singapore University College, London, UK Professor T Kay, Dr H Thomas and Dr Insertional mutagenesis system to identify T Loudovaris Immunology and Diabetes Role of HCN channels in absence epilepsy epigenetic factors that control embryonic Unit, St Vincent’s Institute, Melbourne, stem cells differentiation Dr S Petrou Howard Florey Institute, The VIC University of Melbourne, Melbourne, VIC Dr N Lenka National Centre for Cell Mechanism(s) by which HS preserves Professor S Berkovic Department of Science, Pune University Campus, beta cell viability and protect(s) against Medicine, Austin Health, The University of Ganeshkhind, Pune, India oxidant damage Melbourne, Melbourne, VIC Associate Professor T O’Brien Department of Professor R Stocker and Dr G Dr Daniel Ryan Medicine, The University of Melbourne, Magzhal Victor Chang Cardiac Research Melbourne, VIC Structural and functional analysis of a Institute, Sydney, NSW cancer-linked co-regulator complex Professor Joel Mackay School of Molecular Biosciences, University of Sydney, Sydney, NSW
Annual Review 2013 83 Professor David Tremethick Oxygen-induced retinopathy and 670 nm Analysis of human Tfr cells light The role of histone variants in modulating Professor S Sakaguchi Immunology chromatin fibre dynamics Professor JE Dahlstrom Anatomical Frontier Research Center, Osaka Pathology, ACT Pathology, Canberra University, Osaka, Japan Dr K Luger Department of Biochemistry Hospital and ANU Medical School, The Role of mTOR in Tfh cell metabolism and Molecular Biology, Colorado State Australian National University, Canberra, University, Fort Collins, CO, USA ACT Professor M Boothby Vanderbilt Chromatin remodeling during early University School of Medicine, Nashville, Dr A Kent, Dr R Essex and Dr A-L TN, USA development Mohamed ANU Medical School, The Dr A Peters Friedrich Miescher Institute Australian National University, Canberra, Control of RNA and miRNA homeostasis for Biomedical Research, Basel, ACT and ACT Health, The Canberra Dr R Casellas Genomics and Immunity Switzerland Hospital, Canberra, ACT Section, National Institute of Arthritis Chromatin remodeling during oogenesis and Musculoskeletal and Skin Diseases, Professor Carola G Vinuesa National Institutes of Health, Bethesda, Dr J Bowles Institute for Molecular MD, USA Bioscience, University of Queensland, Role of single nucleotide polymorphisms Brisbane, QLD in the development of systemic lupus Professor Bruce Walmsley Chromatin function in differentiation and erythematosus cancer Dr D Gale Imperial College, London, Mechanisms of central neuronal integration Professor SA Grigoryev Department UK Dr A Bowie Trinity College, Dublin, Professor REW Fyffe and Mr AS of Biochemistry and Molecular Biology, Ireland Dr A Weber The University of Deardorff Boonshoft School of Medicine, Milton S Hershey Medical Centre, Penn Tübingen, Tübingen, Germany Professor Wright State University, Dayton, OH, USA State University College of Medicine, R Brink Immunology Program, Garvan Hershey, PA, USA Institute of Medical Research, Sydney, NSW Associate Professor S Alexander Mr Hao Yang Westmead Hospital, Westmead, NSW Characterization of the interactions Dr Krisztina Valter Dr M Alarcon-Riquelme University of between RNA and ‘moonlighting’ Slow-release treatment options in drug Granada, Granada, Spain Professor P metabolic enzymes in rodent delivery to the retina Petersen Molecular Pathology, University cardiomyocytes and their change in of Tartu, Tartu, Estonia response to stress Professor S Bisti University of L’Aquila, L’Aquila, Italy Neural-like signaling in germinal centre Professor M Hentze European response Molecular Biology Laboratory (EMBL), The effects of VCP treatment in the Heidelberg, Germany degenerative retina Professor C Doglioni Department of Pathology, San Raffaele Hospital, Milan, Professor GJ Kotwal Kotwal Italy Bioconsulting and St Kitts Medical Professor Ian G Young Regulation of Tfh cells by microRNA-146a School, WI, USA Professor GJ Arason Role of IL-3 receptor in myeloid leukaemia University of Iceland, Reykjavik, Iceland Dr D Baltimore Biology and Biological Professor YZ Chen and Dr Y Wu Fujian Engineering, California Institute of Muller cell reactive gliosis and its control, Medical University, Fuzhou, China using in vivo and in vitro models Technology, Pasadena, CA, USA Cytokine receptors Dr M Madigan Save Sight Institute, Roquin and RNA regulation Dr J Murphy Molecular Medicine University of Sydney, Sydney, NSW and Dr J Babon The Walter and Eliza Division, Walter and Eliza Hall Institute of Department of Optometry, University of Hall Institute for Medical Research, Medical Research, Melbourne, VIC NSW, Sydney, NSW Melbourne, VIC Role of cytokines in allergic inflammation 670 nm red light treatment in retinal Tfh metabolism degenerations Professor PS Foster School of Dr J Babon The Walter and Eliza Biomedical Sciences and Pharmacy, Professor JT Eells University of Hall Institute for Medical Research, University of Newcastle, Newcastle, NSW Wisconsin, Milwaukee, WI, USA Melbourne, VIC Professor M Febbraio The effects on 670 nm light in retinal Baker IDI Heart and Diabetes Institutes, damage Melbourne, VIC Assistant Professor RG Jones Department of Physiology, McGill Dr R Essex ANU Medical School, The University, Montreal, QC, Canada Dr A Australian National University, Canberra, Kallies Walter and Eliza Hall Institute of ACT Medical Research, Melbourne, VIC
84 The John Curtin School of Medical Research AUSTRALIAN PHENOMICS FACILITY COLLABORATIONS
Australian Phenomics Australian Phenomics China- Australia Centre of Network Project: ES cell to Network Project: Phenomics Research Mouse Service Australian Phenome Bank Professor H Tang Chinese Academy of Professor I Smyth Department of Cryopreservation Sciences, Institute of Biophysics, Beijing, China Biochemistry and Molecular Biology Dr D Hopwood Animal Resources and Department of Anatomy and Centre, Murdoch, WA Developmental Biology, Monash International Mouse University, Melbourne, VIC Australian Phenomics Phenotyping Consortium Australian Phenomics Network Project: RNAi Professor S Brown (Chair), Harwell MRC, Harwell Science and Innovation Network Project: ENU technologies Campus Oxfordshire, UK Variant Collection Professor R Teasdale Institute for Molecular Bioscience, The University of Melanoma Project and SNV Dr B Kile Walter and Eliza Hall Institute of Queensland, Brisbane, QLD Medical Research, Melbourne, VIC Detection Professor R Johnstone Peter Professor S Foote Menzies Research MacCallum Cancer Centre, Melbourne, Mr C Broadley Australian Genome Institute Tasmania, Hobart, TAS and VIC Research Facility Macquarie University, Sydney, NSW Dr L Winteringham Western Australian Institute for Medical Research, Perth, WA Developing a new imaging Australian Phenomics and brain atlas tool Network Project: Mouse Systems Approach to Professor G Galloway and Dr A Janke Pathology Immunity and Inflammation Centre for Advanced Imaging, National Imaging Facility, University of Queensland, Professor J Furness University of Professor R Ulevitch The Scripps Brisbane, QLD Melbourne, Melbourne, VIC Research Institute, La Jolla, CA, USA Dr T Kuchel South Australian Health and Medical Research Institute, Adelaide, SA Mutagenetix – A database of Framework Data Sets mutations and phenotypes Mr A Gilbert Bioplatforms Australia, Sydney, NSW induced by ENU Professor B Beutler University of Texas Southwestern Medical Center, Dallas, TX, USA
Annual Review 2013 85 86 The John Curtin School of Medical Research Staff and Student lists
Department of Genome Biology Department of Immunology Eccles Institute of Neuroscience Department of Molecular Bioscience Department of Translational Medicine Department of Pathogens and Immunity Australian Phenomics Facility School Administration School Services The ACRF Biomolecular Resource Facility and Genome Discovery Centre Students
Annual Review 2013 87 GENOME BIOLOGY
Professor and Head of Chromatin and Computational Genomics Department Transcriptional Regulation Group Tremethick DJ, BSc(Hons), (USyd), PhD Group Associate Professor and Leader (MacqU) Professor and Leader Huttley GA, BSc(Hons) (MacqU), PhD (UC Departmental Administrator Riverside) Tremethick, DJ, BSc(Hons), (USyd), PhD Stenhouse J, BA (MacqU) Postdoctoral Fellows Research Fellow Kaehler B, BSc (UNSW), BEng(Hons) Alternatives to Animal (UNSW), MQuantitative Finance (UTS), Soboleva T, MSc (MSU), PhD (MSU) Research Through PhD Postdoctoral Fellows Bioinformatics Group Pérez‑Bercoff Å, PhD (Dublin) Cook AJL, BMedSci(Hons) (USyd), PhD Visiting Fellow Associate Professor and Leader (USyd) (until June) Verbyla K, BSc(Hons) (Adel), PhD (UMelb) Lidbury BA, BSc(Hons) (Newcastle), PhD Domaschenz RM, BSc(Hons) (Adel), PhD (until January) (Cambridge) (until February) Visiting Fellows Visiting Student Nekrasov M, PhD (Heidelberg) Gahan M, BA/BSc(Hons), Postgraduate Zhu Y, BSc(Hons), B Comm Diploma in Forensic Science (LaTrobe), Ryan DP, BSc(Hons) (USyd), PhD PhD (Monash) Westman B, BSc(Hons) (USyd), PhD (until Computational and Richardson AM, BA(Hons) (Well), MStats, PhD January) Conceptual Biology Group Visiting Student Visiting Fellows Shang GF, Master of Medical Immunology Cook AJL, BMedSci(Hons) (USyd), Professor and Leader (Tongji Medical University, China) PhD (USyd) (from July) Gready JE, BSc(Hons), PhD (USyd), School Visitor (Statistical Consulting Hapel A FRACI Unit, ANU) Shannon MF, BSc(Hons), PhD (National Senior Research Officers Neeman T, BA (Harvard), MA, PhD (Virginia) University of Ireland) Cummins PL, BSc(Hons), PhD (USyd) Domaschenz RM, BSc(Hons) (Adel), PhD Kannappan B, BSc (Madras), MSc, PhD (Cambridge) (from March) (Pune) Westman B, BSc(Hons) (USyd), PhD Matthews PR, BSc(Hons), PhD (ANU) (from February) Research Officer Howles P, BA (USyd), BSc (Adel), BSc(Hons), PhD (part‑time) Postdoctoral Fellows Alonso‑Cantabrana H, BSc(Hons), PhD (U Alicante) (until January) Vassilieva T, MSc (Novosibirsk) Research Assistants Good G, BSc (MacqU) (part‑time) (until January) Mani‑George A, BSc, (Mahatma Gandhi, India), MSc (Bharathiar, India) Wallace MJ, BSc (Wollongong) Visiting Fellow Armarego W, PhD, DSc (ULond), FRSC, FRACI
88 The John Curtin School of Medical Research Genome Diversity and RNA Biology Group Translational Genomics Health Group Professor and Leader Group (from March) Professor and Leader Preiss T, Dipl Chem (Philipps University, Associate Professor and Leader Easteal, S, BSc(Hons) (St Andrews), Marburg Germany), PhD (Newcastle upon Arcos‑Burgos M, MD, PhD (University of MBA, PhD (Griffith) Tyne), Habil (Heidelberg) Cauca, Colombia) Postdoctoral Fellow Research Fellows Research Assistant Das D, BSc(Hons) (Presidency College, Archer SK, BSc(Hons), PhD Cai Y, BMed (Bejing Medical University, Kolkata), MSc (Madurai Kamaraj), PhD Clancy JL, BSc(Hons) (UNSW), PhD China) (JNCASR, Bangalore) (from April) (UNSW) Senior Technical Officer Postdoctoral Fellows Departmental Visiting Tan X, BSc (Shandong), MSc (China Beveridge N, (UNewcastle) Fellows Pharmaceutical) Liao Y, PhD (UC Davis) Barlin G, PhD, DSc (USyd), FRACI Research Assistants Patel H Cox G, BSc, PhD (UMelb), FAA Prichard Z, BSc(Hons) (UNSW), PhD (until Senior Technical Officer October) Denborough M, MD, ChB (Cape Town), Pagler E, BSc (Santo Tomas) MD (UMelb), DPhil (Oxon), DSc (UMelb), Pryce, K, BAppSci (UHAN, Nijmegen), FRCP BTech (Gilde College, Venlo) (from May) Visiting Fellows Morrison JE, BSc (USyd), MSc (UQ), Shankar R (from July) DPhil (Oxon), DSc Shirokikh N (July‑December) Rodriguez M‑C (September – November)
Annual Review 2013 89 IMMUNOLOGY
Professor and Head of Diabetes/Transplantation Immune Tolerance and Department Immunobiology Laboratory Signalling Laboratory
Goodnow, CC, Goodnow CC, BSc(Vet) Fellow and Laboratory Leader Professor and Laboratory Leader (USyd), BVSc(Hons (USyd), PhD (USyd), FAA, FRS, NAS Simeonovic CJ, BSc(Hons), PhD Goodnow CC, BVSc (USyd), BScVet(Hons) (USyd), PhD (USyd), FAA, Departmental Administrator Visiting Fellows FRS, NAS Weil ETF McCullagh P, MBBS (UMelb), DPhil (Oxon), MRCP (Lond), MD (UMelb) Research Fellow Bertram E, BSc(Hons) (Adel), PhD (Adel) Cancer and Vascular Wilson JD, BSc(Hons), MBBCh, BAO(Hons), MD (Queens, Belfast), MRCP CJ Martin Fellows Biology Group (UK), FRACP Parish IA, BSc(Hons), PhD (WEHI) Professor and Leader Technical Officers Reed JH, BSc(Hons) (Flinders), PhD Parish CR, BAgrSc (UMelb), PhD (UMelb) Brown D, AssDipAppPath (CIT) (casual) (Flinders) (from August)
Research Fellow Hamilton P, Certificate II (Animal Tech) Research Fellows (CIT) (part‑time) Quah B, BSc, PhD Daley S, BVSc (UQ), DPhil (Oxon) Popp SK, BSc, AssDipAppSci (Biol) (CIT) Postdoctoral Fellow (part‑time) Horikawa K, MD (Chiba), PhD (Tokyo) Coupland L, BSc(Hons), PhD, RN Visiting Scholar Visiting Fellows Visiting Fellows Hayashi K (from October) Cornall R, PhD Chong G, MBBS (Hons), BMedSc Fahrer A, BSc(Hons) (UMelb), PhD (Monash) FRACS, FRCS (C) FRCS (E), Immunogenomics Group (UMelb) Diplomate, American Board of Surgery Randall K, MB (UNSW), BS (UNSW), BSc (USA) Professor and Leader (UNSW), FRACP, FRCPA, PhD Cooper PD, BSc (Hons) (ULond), PhD Goodnow CC, BSc(Vet) (USyd), Wilson A, BSc(Hons) (UC), RN (ULond), DSc (ULond) BVSc(Hons) (USyd), PhD (USyd), FAA, Freeman C, BSc(Hons) (Adel), PhD (Adel) FRS, NAS Research Technicians Balakishnan B, BSc(Hons) Hindmarsh EJ, BSc(Hons) (USyd), PhD Laboratory Manager Howard D, BSc Li RW, MD (China Medical University), Townsend M, PathTech Cert (TAFE), PhD (Southern Cross) AssDipAppPath (Bruce TAFE) Law H, BSc Price J, BSc(Hons) (UMelb), PhD (UMelb) Miosge L, BSc(Hons), PhD Staykova M, PhD (USofia) Bioinformatics Laboratory Rayner J, BSc (until December) Warren HS, BSc(Hons), PhD (UQ) Bioinformatics Fellow Sontani Y, BSc(Hons) (Murdoch), PhD Zhang DH, BMed (2nd Military Medical Andrews TD, BSc(Hons), PhD Laboratory Assistant University, PR China) Bioinformaticians Kanazawa Y (casual) Senior Technical Officer Cho V, BSc (Auck), PhD Bezos A, BSc (USyd), MSc (USyd) Field M, BSc (Biol)/BSc (Computer Sci) Technical Officer (UBC) Browne A, BA (ANU), Dip Ed (UNE) Johnson S, BCompSci (part‑time) (from (part‑time) February) Shao Y, BSc(Hons) (until January School Visitor Wang J, MSc (UNSW)
Editorial/Administrative Assistant Parish B, BSc (Madras), BSc, MSc, GradDipCompStudies (UC) (part‑time)
90 The John Curtin School of Medical Research Ramaciotti Immunization Molecular Mucosal Vaccine Translational Research Unit Genomics Group Immunology Group (joint with The Canberra Hospital) Senior Research Fellow and Fellow and Leader Leader Ranasinghe C, BSc (Uni Rouen, France), Professor and Leader Enders A, MD, PhD (Freiburg) MPhil (Uni Colombo), PhD (UWA) Cook M, MB, BS (USyd), FRACP, FRCPA, PhD (USyd) Research Technician Research Fellow Barthel N, DipBiol (Freie Univ, Berlin) Jackson R, BSc(Hons) (Monash), PhD Research Assistants (Edin) Chand R, BSc(Hons) (UMelb) Infection and Immunity Adjunct Fellow Srivastava U, MSc Group Sedger L, BAppSci(Hons) (UTS), PhD Wilson A, BSc(Hons) (UC), RN (part‑time)
Associate Professor and Leader Visiting Fellows Karupiah G, BSc (Hons), MSc (Malaya), Mullbacher A, BSc, MSc (Auck), PhD PhD Stambas J, BSc(Hons) (UMelb), PhD (UMelb) Host Defence Laboratory Technical Officer Associate Professor and Ravichandran J, BSc (India), BSc(Hons) Laboratory Leader (UMelb) (part‑time) Karupiah G, BSc (Hons), MSc (Malaya), Visiting Technical Officer PhD Buchanan A, BHealthSci (UC) Inflammation and Viral Immunopathology Laboratory
Fellow and Laboratory Leader Chaudhri G, BSc(Hons), PhD
Visiting Fellows Belz G, BVBiol (UQ), BVSc(Hons) (UQ), PhD (UQ) (until February) Newsome TP, BSc(Hons) (UMelb), PhD (Inst Molecular Pathology, Vienna) Scalzo A, BSc (Hons) (UMelb), PhD (UMelb) (until March)
Senior Technical Officer Eldi P, MBBS (India), MSc (UQ) (part‑time)
Technical Officer Patel V, PhD (until February)
Annual Review 2013 91 ECCLES INSTITUTE OF NEUROSCIENCE
Professor and Head Development of Visual Human Neuroimaging Stuart GJ, BSc(Hons) (Monash), PhD, Diagnostics of Eye Disease Group FAA Group Associate Professor and Leader Departmental Administrator Professor and Leader James AC, BSc(Hons) (Adelaide), PhD Khalidi D Maddess T, BSc (Hons) (UBC), PhD (ANU) Senior Technical Officer Research Fellows Neural Coding Group Rodda GR, PTC Barbosa M, BSc, MSc, PhD (São Paulo) Carle C, BSc(Hons), PhD Associate Professor and Leader Blood Vessel Group Arabzadeh E, MD, PhD Sabeti F, BOptometry(Hons), Professor and Leader GradCertOcuThera, PhD Postdoctoral Fellow Hill CE, BSc(Hons), PhD, DSc (UMelb) Centre Manager Adibi M, PhD Postdoctoral Fellow Webster B, BSc Research Assistant Howitt L, BSc (USyd), PhD (UNSW) Visiting Fellows Sizemova A Bell A Neuroimmunology & Hughes A Neuronal Network Group Cardiovascular Dysfunction Levick W Associate Professor and Leader Laboratory Mallikarjunan R Stricker C, MD (Zurich), PhD (Bern) Penfold P Leader Research Fellow Haddock RE, BSc (Wollongong), Sarac Ö Cowan AI, BSc(Hons), PhD BSc(Hons), PhD Stronks C Vidovic M Neurophotonics Laboratory Cerebral Cortex Group Xiang F Leader Associate Professor and Leader University Fellow and Daria V, PhD (Osaka University) Bekkers JM, BSc(Hons) (Griffith), MSc Emeritus Professor (Manchester), PhD (Cambridge) Research Assistant Horridge A Sane S (from May) Postdoctoral Fellows Ibbotson M Choy J, BSc (UMS), PhD Suzuki N, BSc (Tsukuba), MMedSci (Tsukuba), PhD (Tokyo)
Research Assistant Tang S‑M (until February)
92 The John Curtin School of Medical Research Neuronal Signalling Group Retinal Cell Damage and Neural Control Systems Repair Group Laboratory Professor and Leader Stuart GJ, BSc (Hons) (Monash), PhD, Professor and Leader Leader FAA Valter K, MD, PhD, GradCert HE Potas JR, BMedSc(Hons) (USyd), PhD (USyd)
Postdoctoral Fellow Brain Development Synapse and Hearing Group Laboratory Biswas S, PhD (from November) Visiting Fellows Professor and Leader Leader Walmsley B, BE, PhD (Monash), DSc Madigan M Song Z‑M, MMSc (Jiamusi), PhD (UNSW) (Flinders) Retinal Development and Visual Neuroscience Group Neuronal Signalling Aging Group Professor and Leader Laboratory Professor and Leader Lamb TD, BE (UMelb), ScD (Cambridge), Professor and Leader Provis J, BSc(Hons) (UNSW) PhD (UNSW) FRS, FAA Stuart GJ, BSc (Hons) (Monash), PhD, Postdoctoral Fellow Visiting Fellow FAA Rutar M, BMedSc (Hons), PhD Jarvinen JLP, MSc (Helsinki), PhD Postdoctoral Fellows (Cambridge) Research Co-ordinator Breton J‑D, MSc, PhD (Strasbourg) Natoli R, BSc (Hons) (USyd), PhD Ikeda K, BA (UC Boulder), PhD University Fellow and Keshavarzi S (from May) Emeritus Professor Curtis DR, AC, MBBS (UMelb), PhD, Visiting Fellows FRACP, FAA, FRS Gabrei S To M‑S
Annual Review 2013 93 MOLECULAR BIOSCIENCE
Professor and Head of Biomolecular Interactions Cancer Metabolism and Department Group Genetics Group
Dulhunty AF, BSc (USyd), PhD, DSc Fellow and Leader Fellow and Leader (UNSW) Casarotto MG, BSc(Hons) (UMelb), PhD Blackburn A, BSc(Hons) (UNSW), PhD Departmental Administrator (UMelb) Laboratory Technician Mitigas R Postdoctoral Fellow Rooke M, BMedSci (Pharm Sci) (CSU) Norris NC, BSc (Adv)(Hons) (USyd), PhD (York) Cardiac and Skeletal Muscle Technical Officer Proteomics Group Aditya S, BBiotech(Hons) Visiting Fellows Leader Beard NA, BAppSci(Hons) (LaTrobe), PhD Fan J, BSc (Fudan), MSc (Fudan), PhD (Auck) Laboratory Technician Lim PS, BMedSci, PhD Janczura, M Zafar A, PhD Technical Assistants Visitor Ayad S, BSc (Assuit, Egypt) (from April) Rosenberg M, PhD Kerdo E, BSc Thekkedam C, BScBioTech(Hons) (Wollongong)
94 The John Curtin School of Medical Research Cytokine Molecular Biology Visiting Fellows Muscle Research Group and Signalling Group Baker R, BSc(Hons) (UNSW), PhD Professor and Leader Coggan M Professor and Leader Dulhunty AF, BSc (USyd), PhD, DSc Dahlstrom J, MBBS(Hons), FPAC, PhD, (UNSW) Young IG, MSc (UMelb), PhD FRCPA, FFOP, FFSc, GradCertEdSt (HigherEd), SFHEA Postdoctoral Fellow Senior Research Advisor Abdus F, BSc(Hons) (Bangladesh), Hayes MT, BSc (USyd), MSc (USyd), Gallant E, PhD (Minnesota) MSc (UWSyd), PhD DipEd (Tert) (UNE), PhD (QUT) (until October) Postdoctoral Fellows Visiting Fellows Lee E, BSc(Hons) PhD Karunasekara YA, MD (USSR), PhD Chen J, BSc, (China), MMed (China), Liu D, PhD (USyd) (from March) Mirza S, BSc(Hons) (Dhaka), PhD (Flinders) MSc(BioTech) (UNSW), PhD Shield A, BBiotech(Hons) (Flinders), PhD Murphy J, BSc, PhD (Flinders) Talukder S, BSc(Hons) (Dhaka), MSc(Dhaka), PhD (Tokushima) (until Visiting Scholar Ramshaw IA, MSc (Brunel), PhD February) Yong W, MMed (China), MD (China) Visiting Scholar Visiting Fellow Technical Officers Takemura K, PhD Tierney L, BSc, MSc (Otago), PhD Dai J, BSc, MBiotech Laboratory Technicians Senior Technical Officer Ewens C Cappello J, BSc (UC), AD AppSciAnSci Pace S, BSc (UTS) Wen B, MB (China), MSc (China), PhD (CIT) Tummala P, BSc (Nagarjuna U) MSc Laboratory Technician Epigenetics and Genome (MSU), PhD (OU) Stivala J Stability Group Stem Cells and Gene Fellow and Leader Targeting Group Rangasamy D, MSc, MTech (India), PhD (UK) Professor and Leader Matthaei KI, BSc(Hons) (UNSW), PhD Molecular Genetics Group Visiting Fellows Professor and Leader Barharvand H Board PG, BSc(Hons), PhD (UNE) Frese M, BSc (Osnabrück), PhD (Freiburg) Postdoctoral Fellow Theodoratos A, BSc(Hons) (USyd) PhD
Annual Review 2013 95 TRANSLATIONAL MEDICINE
Professor and Head of Research Fellows Technical Officer Department Mastronardi CA, PhD (University of Cai Y, BMed (Bejing Medical University, Buenos Aires, Argentina) China) (until September) Licinio J, MD (Universidade Federal da Bahia, Brazil), FAPA (until April) Paz‑Filho G, MD (Universidade Federal do Paraná, Brazil) Translational Genomics Head of Department Technical Officers Group Mastronardi CA, PhD (University of Buenos Aires, Argentina) (from April) Cai Y, BMed (Bejing Medical University, Associate Professor and Group China) (until September) Leader Departmental Administrator Koskinen A (until March) Arcos‑Burgos M, MD PhD (University of Vitler L Visiting Fellows Cauca, Colombia) Translational Medicine Boguszewski CL Technical Officer Group Rettori V Cai Y, BMed (Bejing Medical University, China) (until September) Professor and Group Leader Pharmacogenomics Group Licinio J, MD (Universidade Federal da Bahia, Brazil), FAPA, FRANZCP (until April) Professor and Group Leader Mastronardi CA, PhD (University of Wong M‑L, MD (São Paulo, Brazil), Buenos Aires, Argentina) (from April) FRANZCP
PATHOGENS AND IMMUNITY
Professor and Head of Post-doctoral Fellows Malaria Immunology Group Department Athanasopoulos V, BSc(Hons), PhD (UMelb) Group Leader Vinuesa CG, LMS(MBBS) (Madrid), Cockburn I, MBiochem (Oxon), PhD (Edin) DRCOG (Lond), MSc, PhD (Birmingham) Duan T, MBBS (Nanjing), PhD Sweet R, BSc (UCLA), MSc (Yale), MPhil Postdoctoral Fellow Departmental Administrator (Yale), PhD (Yale) Dups J, BVSc (UQ), PhD (UMelb) (from Morales D Visiting Fellows October) Humoral Immunity and Good M, AO, BSc, MBBS, PhD, MD, DSc Laboratory Technician Autoimmunity Group Walter G, BA (Cantab), MBChB Cai Y, BMed (Beijing Med) (from (Edinburgh), MRCP (UK), MD September)
Elizabeth Blackburn NHMRC Laboratory Technical Staff Research Fellow Cheng T (until October) Vinuesa CG, LMS(MBBS) (Madrid), DRCOG (Lond), MSc, PhD (Birmingham) Williams N
CJ Martin Fellow Research Assistant Ellyard J, BAs, BSc(Hons), PhD Lee SK, MBioTech (Flinders) (April – October)
96 The John Curtin School of Medical Research AUSTRALIAN PHENOMICS FACILITY AND ANIMAL SERVICES
Director Quarantine Coordinator Animal Services Coordinators Winslade SL, BA(Hons) (UNE), PhD (UNE) Ross N, RLAT, Dip AppSci Animal Tech (CIT) Barker A Chief Scientific Officer Quarantine Technician Bolton S‑M Goodnow CC, BVSc(Hons) (USyd), Hunt B Burke H, BBehaveStudies (Swinburne) BScVet (Hons) (USyd), PhD (USyd), Gooding D, Cert IV Training FAA, FRS, NAS Information Technology and Assessment Chief Operating Officer Technicians Sjollema GE, BSc(Hons), MFM IT Manager Allsop I, Cert III Animal Tech (CIT) Wu P, BSc(Hons), BCompSci (Canada) Head of Animal Services (from November) Fowler S, BSc, BVMS (Murdoch) IT Officers Aquino W, BAniSci (UWS) (from Fang Y (until November) December) Head of International Programs Hongyu M Arthur M Bertram E, BSc(Hons) (Adel), PhD (Adel) Quinn G Barker A Boljun M Scientific Business Manager Shaw F Burke J Dobbie MS, BAppSc (UTS), MSc (USyd), Tan J, BCompSci (UA), MComp (from Bush T (from September) PhD (Lond) September) Chau K, Dip Animal Tech (CIT) Liaison Officer Genomics Cover G Hewitt L, BMedSci (UC) Figueroa S (until February) Head of Genomics Research Fowlie C Departmental Administrator Whittle B, BSc(Hons) Haas P O’Keefe R Genomics Coordinators Hamilton R Mann D, BSc(Hons) Hartley S Scientific Programs Hebda M (until July) Zhang Y, BSc, MSc (China) Scientific Programs Manager Irvin M (until November) Balakishnan B, BSc(Hons) Genomics Technical Officers Jarvis L Agrawal P, BSc, MSc, PhD (India) (until Kaya MS, BSc (U Newcastle) Scientific Project Coordinators September) Lin Q Kofler J, DipAnTech (CIT), BSc Armstrong M (from November) Lockley J Morris L, BSc(Hons) (Lond), MSc(Res) Fitzgerald L, AssocDip Animal Sci, Cert IV Lorenzi L Vet Nursing (CIT) (Edin), PhD (Edin) (from December) McWilliam D Gao J, BSc (China), MBiotech(Hons) Tunningley R, BMedSci Petty K (until October) Liang R, BSc, MSc (China) Pobjie A, DipAniTech (CIT) (from July) Scientific Project Technician Liu M, BSc, MSc (China) Portway C (from July) Lorenzo A, BSc Biol (RP) Palkova A, BBus (QUT), BSc(Hons) (UQ) Prewett B Patel V (from July) Reid C (until June) Sakthivel G, BMedSci(Hons) (from Australian Phenome Bank Sibley B November) Sparrow M Phenome Bank Curator Sayeed S, BSc, MSc (Bangladesh), PhD Read SH, BSc(Hons) (Adel), PhD (Adel) (UK) Spencer C Schoning J, BBiotech(Hons) (from July) Steen E (from November) Cryopreservation and IVF Talbot S, Dip AppSci (Animal Tech) (CIT) Coordinator Animal Services (from December) du Boulay C, Cert IV Vet Nursing (CIT), Vithanage U (from November) Dip AppSci (CIT) Animal Services Managers Watson D Bowditch K IVF and Cryopreservation Young S Clydesdale M Technician Building Maintenance Supervisor Thomsen N, BSc(Hons) (UQ) (from April) Hosking A Storeperson/Maintenance Assistant Smith D
Annual Review 2013 97 SCHOOL ADMINISTRATION
Director Outreach and Purchasing Licinio J, MD (Universidade Federal da Communications Unit Dowling E (November – December) Bahia, Brazil) FAPA (until April) Enright L (until April) Parish CR, BAgrSc (UMelb), PhD (UMelb) Manager (from April) Nicol MJ, BSc (Wollongong), BSc(Hons), Townsend K (May – November) PhD Wozniak P, Cert IV GPC Deputy Director Board PG, BSc(Hons), PhD (UNE) Senior Multimedia Officer Edwards K, PhotCert Safety & Training Business Manager Advisors Lillicrap G, FCPA, FIIA (Aust) Education Glasson S (until October) Executive Assistant to Director Associate Director Education Hayes MT, BSc (USyd), MSc (USyd), Vitler L DipEd (Tert) (UNE), PhD (QUT) (from and Medical Sciences Graduate October) Reception Convener Azzopardi N (from January) Cowan A, BSc(Hons), PhD, GradCertHE Patel A (until January) Student Administrator Riley W
SCHOOL SERVICES
Facilities and Services Microscopy and Cytometry Technical Services Resource Facility (MCRF) Manager Manager Coombes D Head Coombes D Operations Assistant Gillespie CM, GradCertMic (USyd) Technical Officers Ciuffetelli L Histologist Cremer P Senior Storeperson Prins AS, BAppSci (RMIT) Gair L Clements R Flow Cytometry Specialist Jordan T Kynoch M Storeperson Vohra H, MSc(Hons) (Punjab), PhD (PGIMER) Percival M Talbot A Flow Cytometry and Microscopy Rhall G Media & Washup Operator Scharrer E Devoy M, BSc(Hons) Administrative Assistant Manager Dowling E Coombes D
Supervisor and Technical Officer Gilmartin L, Cert III Lab Skills (CIT) Technician Munday K
98 The John Curtin School of Medical Research THE ACRF BIOMOLECULAR RESOURCE FACILITY AND GENOME DISCOVERY UNIT
Manager Parker B, BSc (UQ), MBBS (UQ), PhD Palmer S, BSc(Hons), MBA (Tech (USyd) Management) (La Trobe) Patel H, BPharm, MBiotech, PhD
Technical Specialist Honorary Visitors Milburn P, BSc(Hons), PhD (Sheffield) Gock A, BAppSci (CSU) Genomics Team Rodriguez M‑C, (September‑November) Cripps T, BSc(Hons) Administrative Assistants Higgins A, CBLT (USQ) Stenhouse J, BA (until March) Ohms S, MBChB, ME, PhD (Auck) Jack M, BA(Hons) (Wellington) (April – Peng K, PhD (Wuhan) October) Zhang K, MSc (Fudan), PhD
Proteomics Team McAndrew K, AssDipAppSci (UC) Zhang K, MSc (Fudan), PhD
Genome Discovery Unit Buckley B, BA(Hons) (UQ) Chuah A, BEng(Hons) (NUS) Jack C, BSc (Wellington)
Annual Review 2013 99 STUDENTS
PhD Students Huang H, BBiomedSci(Hons) (Otago) Samarasinghe K Achuthan S Hussain M, BSc(Hons) Saxena K, BBiotech Agahari F, BSc(Hons) (Japan) Hynes C, BSc(Hons) Seamons J Albarracin R (until September) Jeelall Y, BBiomedSci(Hons) (UMelb) Shafik A Ali E Ji J, BSc(Hons) (USyd) (until September) Shen Q, MBiochem Al Rumaih Z, MSc (Biotech) Jiang S, MBBS FRACP Sibbritt T, BSc(Hons) Alshekaili J, BSc, MD Johnson-Saliba M, PhD Simon Davis DA, BSc(Hons) (Adel) Agrawal A, BSc, MSc (JNKVV, Jabalpur) Jones S, BMedSci(Hons) (UNSW) Singh M, BSc(Hons) (Otago) Dipl Bioinformatics (BAB, Bangalore) Khanna M, BBiomedSci(Hons) (Victoria, Sinha D, BSc, MSc (India) Andrews, S Wellington) Soetanto, R Anuar ND, BSc(Hons), MSc (Malaysia) Kingston Z Srivastava M, MSc (Rewa) Bell S, BSc(Hons) Kolic M Stojakovic A (until October) Bergmann H, D.Vet.Med. (Hanover, Lee C Tan A, BBiotech(Hons) (until October) Germany) Lee E, BSc, MSc (UC) Tan X, BSc, MSc Bock T, BSc(Hons) Lee S, BBiomedSci, MSc (Auck) Townsend D, BAppSci(Hons) (UC), Chan S-Y, BBiotech(Hons) (until May) Li J, BMedSci(Hons) (until November) BForensicSci (UC) Chaston D, BSc(Hons) Li L, BSc(Biotech) Tran L Cho E, BSc (Auck), GradDipSci (Auck) Mapp S, MBBS (UMelb), FRACP, FRCPA Trivedi S, BBiotech, MBiotech (Research) Choong FJ, BMedSci(Hons) (until July) (until August) Tuazon J, BSc, MD (until February) Dalefield M Mei Y, BSc(Hons) (Ottawa) Vassilieva T, MSc (Novosibirsk) (until June) Da Silva E Menon D, BBiotech, MBiotech Velez J Dave P Mishra A, BSc, MSc (Nepal) Wang J, BBiomedSc(Hons) (UMelb), Eldi P, MBBS (India) MSc (UQ) Mohan A, BTech (India) BSc(Hons) (UMelb) Fernando N Morse A Wijesundara D, BBiotech(Hons) Galgamuwe R, MBBS, MSc (Sri Lanka) Morton S, BSc(Hons) Willemse H, BSc(Hons), MSc Gang B, BBiomedSci (Otago) Newman S, BSc(Hons) Wium E, BMedSci(Hons) Go M-A BSc, MSc (U Philippines) Papa I Wong R (until May) Han SY, BSc(Hons) Pratama A, PhB(Hons) Xi Y, BEng (Jilin, China), MSc (Biotech) (Flinders) (until February) Hadjincalou A (until March) Ramiscal R, BSc(Hons) Yabas M, MPhil Hanna A, BSc(Hons) Ratnadikawara M Yang H Hausner S Rebbeck R, BMedSci(Hons) Yap J, MBioTech(Hons) Hu Daniel, BMedSci(Hons) (USyd) Robertson J, BSc(Adv)(Hons) Yoon J Hu Di Rudinski S, BSc(Hons) Sabouri Z, MD
100 The John Curtin School of Medical Research Honours Students MNeuroscience Students MTranslational Medicine Brookes T Burton C Students Chen T Choi B Alexander P Cook S Espinoza-Oyarce D Barry P El Eryani G Hammado N Dunn C Emerson K Haque F Forster R Hasan M Kaide J Li Z Jiao H Martenzon H Rodriguez A Johar A Mehdi A Zhao S Lee J Owens-Walton C Micallef A Pillai E Research Placement Moran I Rajput S Aw V Racic T Ravi I Bruggeman K Sane S Senga S Fam H Spierings E Sharp G Fernandez P Steele S Zhang T Kaya S Wallace D Zinamidar A Li J Worley M Shallal M Zhu I Silva A Sofoulis L Ugool M
MPhil Students Choi S (until August) Li Z Pearce J Zhang Y
MBiotech (Research) Student Dhounchak S
Annual Review 2013 101 102 The John Curtin School of Medical Research Publications, Presentations and Community Outreach 2013
The 2013 list of publications from The John Curtin School of Medical Research includes peer reviewed journal articles reviews, short communications and book chapters. Listed here are the presentations made by staff and students of JCSMR at local, national and international research institutions, conferences and workshops throughout the year. JCSMR staff and students continue to sit on numerous editorial boards, to assist with national and international granting bodies as expert assessors, and to belong to many scientific societies. They are also involved with community activities outside the School. Here we list some of these activities.
Annual Review 2013 103 PUBLICATIONS
Adibi, M, Clifford, CWG and Arabzadeh, Berglund, LJ, Avery, DT, Ma, CS, Moens, Carle, CF, James, AC and Maddess, E (2013) Informational basis of sensory L, Deenick, EK, Bustamante, J, Boisson- T (2013) The pupillary response to color adaptation: Entropy and single-spike Dupuis, S, Wong, M, Adelstein, S, and luminance variant multifocal stimuli. efficiency in rat barrel cortex. Journal of Arkwright, PD, Bacchetta, R, Bezrodnik, Investigative Ophthalmology & Visual Neuroscience 33(37):14921-14926 L, Dadi, H, Roifman, CM, Fulcher, DA, Science 54(1):467-475 Adibi, M, McDonald, JS, Clifford, CWG Ziegler, JB, Smart, JM, Kobayashi, M, Caruana, G, Farlie, PG, Hart, AH, and Arabzadeh, E (2013) Adaptation Picard, C, Durandy, A, Cook, MC, Bagheri-Fam, S, Wallace, MJ, Dobbie, improves neural coding efficiency despite Casanova, JL, Uzel, G and Tangye, SG MS, Gordon, CT, Miller, KA, Whittle, increasing correlations in variability. (2013) IL-21 signalling via STAT3 primes B, Abud, HE, Arkell, RM, Cole, TJ, Journal of Neuroscience 33(5):2108- human naive B cells to respond to IL-2 Harley, VR, Smyth, IM, and Bertram, JF 2120A to enhance their differentiation into (2013) Genome-wide ENU mutagenesis plasmablasts. Blood 122(24):3940-3950 Albarracin, R, Natoli, R, Rutar, M, in combination with high density SNP Valter, K and Provis, J (2013) 670 Bergmann, H, Yabas, M, Short, A, analysis and exome sequencing provides nm light mitigates oxygen-induced Miosge, L, Barthel, N, Teh, CE, Roots, rapid identification of novel mouse models degeneration in C57BL/6J mouse retina. CM, Bull, KR, Jeelall, Y, Horikawa, K, of developmental disease. PLoS ONE BMC Neuroscience 14:125 Whittle, B, Balakishnan, B, Sjollema, 8(3):e55429 G, Bertram, EM, Mackay, F, Rimmer, AJ, Aldahmash, A, Atteya, M, Elsafadi, Castello, A, Fischer, B, Hentze, MW and Cornall, RJ, Field, MA, Andrews, TD, Preiss, T (2013) RNA-binding proteins M, Al-Nbaheen, M, Al-Mubarak, HA, Goodnow, CC and Enders, A (2013) Vishnubalaji, R, Al-Roalle, A, Al-Harbi, in Mendelian disease. Trends in Genetics B cell survival, surface BCR and BAFFR 29(5):318-327 S, Manikandan, M, Matthaei, KI and expression, CD74 metabolism, and CD8 – Mahmood, A (2013) Teratoma formation dendritic cells require the intramembrane Castiblanco, J, Arcos-Burgos, M and in immunocompetent mice after endopeptidase SPPL2A. The Journal of Anaya, JM (2013) What is next after the syngeneic and allogeneic implantation of Experimental Medicine 210(1):31-40 genes for autoimmunity? BMC Medicine germline capable mouse embryonic stem 11:197 Board, PG and Menon, D (2013) cells. Asian Pacific Journal of Cancer Chaston, DJ, Baillie, BK, Grayson, Prevention 14(10):5705-5711 Glutathione transferases, regulators of cellular metabolism and physiology. TH, Courjaret, RJ, Heisler, JM, Lau, KA, Alsharifi, M, Koskinen, A, Biochimica et Biophysica Acta Machaca, K, Nicholson, BJ, Ashton, Wijesundara, DK, Bettadapura, 1830(5):3267-3288 A, Matthaei, KI and Hill, CE (2013) J and Mullbacher, A (2013) MHC Polymorphism in endothelial connexin40 Class II-Alpha chain knockout mice Bocedi, A, Fabrini, R, Farrotti, A, Stella, L, enhances sensitivity to intraluminal support increased viral replication that is Ketterman, AJ, Pedersen, JZ, Allocati, N, pressure and increases arterial stiffness. independent of their lack of MHC Class Lau, PCK, Grosse, S, Eltis, LD, Ruzzini, Arteriosclerosis Thrombosis and Vascular II cell surface expression and associated A, Edwards, TE, Morici, L, Del Grosso, Biology 33(5):962-970 E, Guidoni, L, Bovi, D, Lo Bello, M, immune function deficiencies. PLoS One Chen, GB and Shannon, MF (2013) 8(6):e68458 Federici, G, Parker, MW, Board, PG and Ricci, G (2013) The impact of nitric oxide Transcription factors and Th17 cell Andrews, TD, Sjollema, G and toxicity on the evolution of the glutathione development in experimental autoimmune Goodnow, CC (2013) Understanding transferase superfamily. A proposal for encephalomyelitis. Critical Reviews in the immunological impact of the human an evolutionary driving force. Journal of Immunology 33(2):165-182 mutation explosion. 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Wiley Interdisciplinary (JE-ADVAX) formulated with delta inulin hepatitis virus immunoassay results from Reviews-RNA 4(4):397-422 adjuvant provides robust heterologous routine pathology laboratory assays in Simeonovic, CJ and Parish, CR (2013) protection against West Nile encephalitis unbalanced data. BMC Bioinformatics Comment on: Korpos et al. The Peri- via cross-protective memory B cells and 14:206 islet Basement Membrane, a Barrier neutralizing antibody. Journal of Virology Rodríguez, AJ, Mastronardi, C and to Infiltrating Leukocytes in Type 1 87(18):10324-10333 Paz-Filho, G (2013) Leptin as a risk Diabetes in Mouse and Human. Diabetes Pratama, A, Ramiscal, RR, Silva, DG, factor for the development of colorectal 62(2):531-542 Das, SK, Athanasopoulos, V, Fitch, cancer. Translational Gastrointestinal Simeonovic, CJ, Ziolkowski, AF, J, Botelho, NK, Chang, PP, Hu, X, Cancer 2(4):211-222 Wu, Z, Choong, FJ, Freeman, C Hogan, JJ, Mana, P, Bernal, D, Korner, Sabeti, F, James, AC, Essex, RW and Parish, CR (2013) Heparanase H, Yu, D, Goodnow, CC, Cook, MC and Maddess, T (2013) Dichoptic and autoimmune diabetes. Frontiers in and Vinuesa, CG (2013) Roquin-2 shares multifocal visual evoked potentials identify Immunology 4:471 functions with its paralog Roquin-1 in local retinal dysfunction in age-related Simon Davis, DA and Parish, CR the repression of mRNAs controlling macular degeneration. Documenta (2013) Heparan sulfate: a ubiquitous T follicular helper cells and systemic Ophthalmologica 126(2):125-136 glycosaminoglycan with multiple roles in inflammation. Immunity 38(4):669-680 Sabeti, F, James, AC, Essex, RW immunity. Frontiers in Immunology 4:470 Provis, JM, Dubis, AM, Maddess, T and and Maddess, T (2013) Multifocal Smith, JJ, Vetter, I, Lewis, RJ, Peigneur, Carroll, J (2013) Adaptation of the central pupillography identifies retinal S, Tytgat, J, Lam, A, Gallant, EM, Beard, retina for high acuity vision: Cones, the dysfunction in early age-related macular NA, Alewood, PF and Dulhunty, AF (2013) fovea and the avascular zone. Progress in degeneration. Graefes Archive for Multiple actions of {varphi}-LITX-Lw1a on Retinal and Eye Research 35:63-81 Clinical and Experimental Ophthalmology ryanodine receptors reveal a functional link 251(7):1707-1716 between scorpion DDH and ICK toxins. Proceedings of the National Academy of Sciences of the United States of America 110(22):8906-8911
108 The John Curtin School of Medical Research Sosa-Macias, M, Lazalde-Ramos, BP, Vinuesa, CG and Chang, PP (2013) Xu, Y, Ohms, SJ, Li, Z, Wang, Q, Gong, Galaviz-Hernandez, C, Rangel-Villalobos, Innate B cell helpers reveal novel types of GM, Hu, YQ, Mao, ZY, Shannon, MF H, Salazar-Flores, J, Martinez-Sevilla, VM, antibody responses. Nature Immunology and Fan, JY (2013) Changes in the Martinez-Fierro, ML, Dorado, P, Wong, 14(2):119-126 expression of miR-381 and miR-495 are ML, Licinio, J and LLerena, A (2013) Vinuesa, CG, Fagarasan, S and Dong, C inversely associated with the expression Influence of admixture components (2013) New territory for T follicular helper of the MDR1 gene and development of on CYP2C9*2 allele frequency in eight cells. Immunity 39(3):417-420 multi-drug resistance. PLoS One 8(11): indigenous populations from Northwest e82062 Wang, J, Jeelall, Y, and Horikawa, Mexico. Pharmacogenomics Journal Ye, YXH, Woolfit, M,Huttley, GA, 13(6):567-572 K (2013) Emerging targets in human lymphoma: targeting the MYD88 Rances, E, Caragata, EP, Popovici, J, Stepensky, P, Keller, B, Buchta, M, mutation. Blood and Lymphatic Cancer: O’Neill, SL and McGraw, EA (2013) Kienzler, AK, Elpeleg, O, Somech, R, Targets and Therapy 3:53-61 Infection with a virulent strain of wolbachia Cohen, S, Shachar, I, Miosge, LA, disrupts genome wide-patterns of Schlesier, M, Fuchs, I, Enders, A, Eibel, Wang, ND, Yuan, AW, Deng, ZB, Yang, cytosine methylation in the mosquito H, Grimbacher, B and Warnatz, K (2013) Q, Ma, J, Tan, QH, Zhang, SZ, Xue, Aedes aegypti. PLoS One 8(6):e66482 Deficiency of caspase recruitment domain LQ and Cui, SL (2013) Engineering the male-specificity of Fab against SDM Yin, L, Song, J, Board, PG, Yu, Y, Han, family, member 11 (card11), causes X and Wei, J (2013) Characterization profound combined immunodeficiency in antigen by chain shuffling. Theriogenology 79(8):1162-1170 of selenium-containing glutathione human subjects. Journal of Allergy and transferase zeta1-1 with high GPX activity Clinical Immunology 131(2):477 Warren, HS, Wu, F, Horn, PL, Pyne, prepared in eukaryotic cells. Journal of Sudta, P, Kirk, N, Bezos, A, Gurlica, DB, West, NP and Cripps, AW (2013) Molecular Recognition 26(1):38-45 A, Mitchell, R, Weber, T, Willis, AC, Peripheral blood natural killer (NK) cell Prabpai, S, Kongsaeree, P, Parish, CR, function in healthy adults assessed using Suksamrarn, S and Kelso, MJ (2013) the target-induced NK loss (TINKL) assay. Synthesis, structural characterisation, and Journal of Immunological Methods preliminary evaluation of non-indolin-2- 392(1-2):68-70 one-based angiogenesis inhibitors related Watson, CA, Vine, KL, Locke, JM, to Sunitinib (Sutent (R)). Australian Journal Bezos, A, Parish, CR and Ranson, M of Chemistry 66(8):864-873 (2013) The antiangiogenic properties of Tahiliani, V, Chaudhri, G, Eldi, P and sulfated – cyclodextrins in anticancer Karupiah, G (2013) The orchestrated formulations incorporating 5-fluorouracil. functions of innate leukocytes and Anti-Cancer Drugs 24(7):704-714 T cell subsets contribute to humoral Wiede, F, Fromm, PD, Comerford, I, Kara, immunity, virus control, and recovery from E, Bannan, J, Schuh, W, Ranasinghe, secondary poxvirus challenge. Journal of C, Tarlinton, D, Winkler, T, McColl, SR Virology 87(7):3852-3861 and Korner, H (2013) CCR6 is transiently Teh, CE, Horikawa, K, Arnold, CN, upregulated on B cells after activation and Beutler, B, Kucharska, EM, Vinuesa, modulates the germinal center reaction in CG, Bertram, EM, Goodnow, CC and the mouse. Immunology and Cell Biology Enders, A (2013) Heterozygous mis- 91(5):335-339 sense mutations in Prkcb as a critical Wijesundara, DK, Jackson, RJ, determinant of anti-polysaccharide Tscharke, DC and Ranasinghe, C (2013) antibody formation. Genes and Immunity IL-4 and IL-13 mediated down-regulation 14(4):223-233 of CD8 expression levels can dampen Tse SW, Cockburn IA, Zhang H, anti-viral CD8(+) T cell avidity following Scott AL and Zavala F (2013) Unique HIV-1 recombinant pox viral vaccination. transcriptional profile of liver-resident Vaccine 31(41):4548-4555 memory CD8+ T cells induced by Wijesundara, DK, Tscharke, DC, immunization with malaria sporozoites. Jackson, RJ and Ranasinghe, C (2013) Genes and Immunity 14(5):302-9 Reduced interleukin-4 receptor alpha Verbyla, KL, Yap, VB, Pahwa, A, expression on cd8(+) T cells correlates Shao, YL and Huttley, GA (2013) The with higher quality anti-viral immunity. embedding problem for Markov models PLoS One 8(1):11 of nucleotide substitution. PLoS One 8(7):e69187
Annual Review 2013 109 PRESENTATIONS
Dr Ehsan Arabzadeh Associate Professor Phenotyping Pipeline at the Australian Phenomics Facility Population coding and sensory John M Bekkers th adaptation 60 Japanese Society for Laboratory Odor-evoked responses of identified Animal Science, Tsukuba, Japan Keynote Speaker: International interneurons in the piriform cortex in vivo Brain Research Organisation (IBRO) Commercialisation – An Australian Gordon Conference: Inhibition in the Perspective Neuroscience Summer School, Tehran, Central Nervous System, Les Diablerets, Iran Switzerland Translational Research and Neural coding and efficiency in rats Entrepreneurship Short Course at Indiana Smells interesting: The surprising University, Indianapolis, IN, USA Computational and Systems complexity of the piriform cortex The Australian Missense Mutation Library: Neuroscience (COSYNE) Workshops, Salt Center for Neuroscience, University of Lake City, UT, USA an immediate source of thousands of new California Davis, Davis, CA, USA mouse models to study human disease Neural coding in the rat whisker barrel system Infrafrontier/ International Mouse Dr Andrew Bell Phenotyping Meeting, CNR JCSMR School Seminar Series, The John Headquarters, Rome, Italy Curtin School of Medical Research, ANU, Resonance or travelling wave? An Canberra, ACT alternative interpretation of cochlear mechanics Dr Anneke Blackburn Neuronal encoding in rat whisker-barrel system – from sensory representation to Eccles Institute of Neuroscience Seminar Dichloroacetate: from environmental behaviour Series, The John Curtin School of Medical hazard to cancer therapy Research, The Australian National th QBI Neuroscience Seminar, Queensland University, Canberra, ACT 6 Annual Scientific Meeting of the Brain Institute, Brisbane, QLD Australasian College of Toxicology and Theories of cochlear mechanics Risk Assessment, Canberra, ACT Associate Professor Live video presentation: Institute of Invited speaker Physiology and Pathology of Hearing, Mauricio Arcos-Burgos Warsaw, Poland Calvary ACT MRI Health Forum, Calvary Hospital, Bruce, ACT Translational Genomics: On the Right Track Dr Edward M Bertram Targeting cancer metabolism with Key Note Speaker: Colombian Congress dichloroacetate of the Biology Society, Colombia Next-Gen Mouse Models for Fred Hutchison Cancer Research Center, Understanding Human Disease Genetic Isolates & Human Disease Seattle, WA, USA Joint Conference of Human Genetics Department of Anthropology, The Australian Targeting cancer metabolism with Meeting / International Congress of National University, Canberra, ACT dichloroacetate Genetics: Genetics and Genomics of Global Health and Sustainability, School of Biology University of Dr Vicki Athanasopoulos Singapore Massachusetts, Amherst, MA, USA Functional overlap of ROQUIN-1 and Next-gen ENU Mouse Models for ROQUIN-2 in the repression of mRNAs understanding human disease Mr Bob Buckley controlling TfH cell accumulation and systemic inflammation Korean Institute of Science and Data describing Autism Spectrum Technology, Seoul, Republic of Korea Disorder in Australia: information relating th 15 International Congress of to diagnoses, prevalence, service access Immunology, Milan, Italy Next-gen ENU Mouse Models for understanding human disease and outcomes Dr Marconi Barbosa Yonsei University College of Medicine, APAC’13 The Asia Pacific Autism Conference, Adelaide, SA Locally countable properties and the Seoul, Republic of Korea perceptual salience of textures Next-gen ENU Mouse Models for Introducing Bioinformatics Australasian Ophthalmology and Vision understanding human disease The John Curtin School of Medical Science Meeting, Hobart, TAS Korean Research institute of Bioscience Research, The Australian National and Biotechnology, Ochang, Republic of University, Canberra, ACT Korea
110 The John Curtin School of Medical Research Dr Corinne Carle Dr Adam Cook Dr Vincent Daria Variation in pupillary responsiveness with A balancing act between the chaperone Advancing neurophotonics using 4D age and visual field location NASP and lysosomal degradation to fine- photostimulation Australian Ophthalmic and Visual tune histone supply and maintain genome Medical University of Innsbruck, Sciences Meeting (AOVSM), Hobart, TAS integrity Innsbruck, Austria It’s not what you show, it’s the way that ComBio 2013, Perth, WA Brain oxygenation and vascular imaging you show it: Stabilising midbrain gain- Session Chair: European control in multifocal pupil perimetry Professor Matthew C Cook Conferences on Biomedical Optics: Canberra Health Annual Research Genetics of primary antibody deficiency Neurophotonics, Munich Germany Meeting (CHARM), Canberra, ACT The Royal College of Pathologists of Efficient neuronal excitation using Australasia Pathology Update, Melbourne, wavefront-corrected holographic Dr Julian Choy VIC photostimulation Mapping of intracortical circuitry in the Germinal centres European Conferences on Biomedical Optics: Neurophotonics, Munich, anterior piriform cortex using optogenetics th 19 Annual Institute for Clinical Pathology Germany Sensory Neuroscience Symposium, and Medical Research Immunology University of Western Sydney, Workshop, The Royal College of Spatio-temporal light modulation for Campbelltown, NSW Pathologists of Australasia, Westmead neurophotonics Hospital, Sydney, NSW Seminar Speaker: European Laboratory Mr Aaron Chuah for Non-linear Spectroscopy, Florence, Ital Dr Lucy Coupland Spatio-temporal light modulation for TriPyGDU: Transcriptome-RNAseq neurophotonics and quantum microscopy Interactive Python Graphical Data Unifier Manipulation of self-recognition Seminar Speaker: Technical University of Lorne Genomics Conference, Lorne, VIC systems to prevent antibody-mediated thrombocytopenias Denmark, Lyngby, Denmark th Quantum probing of living cells Associate Professor The 4 Bootes Course on Translational Medicine: The Pathway from Dicovery to Asia Communications and Photonics Ian Cockburn Healthcare, The John Curtin School of Conference, Beijing, China Visualizing Immunity to Plasmodium liver Medical Research, The Australian National University, Canberra, ACT Four-dimensional probing of neurons in stages brain tissue 14th Frank and Bobbie Fenner Dr Stephen Daley Shaping the Waves: Engineering Optical Conference: Perspectives on Immune Waveform for Biomedical imaging, Recognition, The John Curtin School of Delineating clonal deletion from Howard Hughes Medical Research Medical Research, The Australian National T-regulatory cell differentiation in CD4+ Institute, Janelia Farm, VA, USA University, Canberra, ACT thymocytes It’s moving… it’s alive: What movies can 43rd Annual Scientific Meeting, Dr Michael Dobbie tell us about immunity to malaria Australasian Society for Immunology, The Australian Phenomics Network: School Seminar, The John Curtin School Wellington, New Zealand Creating and Delivering New Research of Medical Research, The Australian Foxp3+ T-reg cells are a cytokine- Tools and Resources National University, Canberra, ACT dependent bi-product of the CCR7+ 11th Transgenic Technology Meeting, Immunity to Malaria – from immunization wave of thymic clonal deletion Guangzhou, PR China to basic science and back again ACT and NSW Australasian Society of Phenomics - Ways and Means Plenary Speaker ACT and NSW Immunology Annual Branch Retreat, Australasian Society of Immunology Bowral, NSW European Molecular Biology Laboratory Annual Branch Retreat, Bowral, NSW Australia PhD Course, Melbourne, VIC In vivo imaging reveals destruction of What is the APN? malaria liver-stages by local action of South Australian Health and Medical CD8+ T cells Research Institute, Adelaide, SA rd 43 Annual Scientific Meeting, The Next-Gen Mouse and the Missense Australasian Society for Immunology, Mutation Library Delivering New Wellington, New Zealand Resources for Understanding Human Disease 27th International Mammalian Genome Conference, Salamanca, Spain
Annual Review 2013 111 Professor Simon Easteal The IgD Enigma: Removal of self-reactivity Mrs Angela Higgins from antibodies on IgD+ IgMlow anergic The National Centre for Indigenous B cells Sequencing trouble shooting Genomics 14th Frank and Bobbie Fenner CSIRO Plant Industry, Canberra, ACT Life Sciences Institute, National University Conference: Perspectives on Immune of Singapore, Singapore Recognition, The John Curtin School of Professor Caryl Hill The National Centre for Indigenous Medical Research, The Australian National Genomics University, Canberra, ACT T-type calcium channels and the vasculature Wellcome Trust Centre for Human IgD, anergy and the germinal center vs Genetics, University of Oxford, Oxford, UK plasma cell decision Danish Cardiovascular Research Academy Annual Symposium, The The American Association of Sandbjerg Estate, Sønderborg, Denmark Dr Anselm Enders Immunologists Annual Meeting - Immunology 2013 NIAID Symposium: Endothelial gap junctions contribute to the The intramembrane protease SPPL2A Current Progress: Development and pathogenesis of arterial disease promotes B cell and CD8- DC survival by Maintenance of Long-Lived Plasma Cells, Keynote speaker: Professorial cleavage of MHC invariant chain Honolulu, HI, USA Inauguration Symposium, University of th 14 Frank and Bobbie Fenner The IgD Enigma: Removal of self-reactivity Southern Denmark, Odense, Denmark Conference: Perspectives on Immune from antibodies on IgD+ IgMlow anergic Nitric oxide deficit elicits oxidative stress Recognition, The John Curtin School of B cells and augments T-type calcium channel Medical Research, The Australian National th contribution to vascular tone University, Canberra, ACT 15 International Congress of Immunology, Milan, Italy Experimental Biology 2013 Symposium: Using mice to understand the human Oxidative Stress, Boston, MA, USA immune system Non-Burnetian mechanisms for actively acquiring tolerance after exposure to Blood flow and tissue perfusion: Cunning th The 4 Bootes Course on Translational foreign antigens control through electromechanical Medicine: The Pathway from Dicovery to coupling Healthcare, The John Curtin School of 2013 Fall Conference of the Korean Medical Research, The Australian National Association of Immunologists, Seoul, Eccles Institute of Neuroscience Seminar University, Canberra, ACT Republic of Korea Series, The John Curtin School of Medical Chris Parish: Chemistry and immunology Research, Australian National University, B-cell survival, surface BCR and BAFFR Canberra, ACT expression, CD74 metabolism and Symposium: Chris Parish ASI life CD8- DCs require the intramembrane membership: A celebration, The John Towards treatment of therapy resistant endopeptidase SPPL2A Curtin School of Medical Research, The hypertension Keystone Symposium: B Cell Australian National University, Canberra, Heart Foundation Willing Hearts Club Development and Function, Keystone, ACT Event, Phillip, ACT CO, USA Professor Jill E Gready Dr Keisuke Horikawa Professor Chris C Goodnow Improving Rubisco performance Single amino acid change in Tnfaip3 IgD and removal of self cross-reactivity ANU Photosynthesis Initiative International inactivates its DUB activity and from antibodies Advisory Group Workshop, The Australian dysregulates B cell responses Institute for Immunity, Transplantation and National University, Canberra, ACT Keystone Symposium: B Cell Infection, Stanford, CA, USA Development and Function, Keystone, CO, USA Clonal Anergy, the Function of IgD, and Dr Rebecca Haddock Autoimmunity and Lymphoma Redemption of Self-Reactive Antibodies in The role of NOX2 in sympathetic Germinal Centers hyperinnervation and underlying obesity- Cancer Collaborative Group Seminar, Keystone Symposium: B Cell Development related hypertension Translational Research Institute, Brisbane, QLD and Function, Keystone, CO, USA Australian & New Zealand Microcirculation Society / Australian Vascular Biology Society Joint Scientific Meeting, Barossa Valley, SA NGF-producing immune cells drive sympathetic neurogenic hypertension Canberra Hospital Annual Research Meeting, Canberra, ACT
112 The John Curtin School of Medical Research Associate Professor Life Exists Beyond GTR Bioinfosummer From lab coat to high dimension feature 2013 Workshop: Flexible Molecular space – An example of bioinformatics in Gavin Huttley Evolution Modelling in Python, The biomedical research Sequence, Assemble, Annotate, Align ... University of Adelaide, Adelaide, SA Continuing Education series, IP Australia, Species Tree? Woden, ACT Keynote Presentation; Genetics Society of Professor Trevor Lamb Australasia Conference, The University of The origin and evolution of vertebrate Dr Yalin Liao New South Wales, Randwick, NSW photoreceptors Impact of growth factors on early Sequence, Assemble, Annotate, Align ... FASEB Summer Research Conference: development Species Tree? Biology and Chemistry of Vision, Faculty of Health Seminar, University of Australasian Mycological Society Steamboat Springs, Colorado, USA Canberra, Bruce, ACT Conference, Adelaide Convention Centre, Adelaide, SA Associate Professor Introduction to programming for scientists Professor Ted Maddess Brett Lidbury using Python Novel diagnostic targets in glaucoma: TIC Presentation, The John Curtin School The 3Rs in Australia and New Zealand: Targeting multifocal pupil perimetry in of Medical Research, The Australian Places to explore and some surprises glaucoma National University, Canberra, ACT EU Science: Global Challenges & World Glaucoma Congress, Vancouver, Extracting biological information from Global Cooperation meeting: Worldwide BC, Canada Implementation of the 3Rs in regulatory sequence variation ORIA plenary: Highlights of Australian toxicology: What are the leadership EMBL Australia PhD program, Walter and research, on Professor Peter Bishop and challenges and opportunities? EU Eliza Hall Institute of Medical Research, ORIA Parliament, Brussels, Belgium Melbourne, VIC 45th Royal Australian and New Zealand Replacing animals in fundamental medical Extracting information from DNA College of Ophthalmologists Congress, research: Will big data and complex sequences using models of sequence Hobart, TAS computer models provide solutions? evolution World Health Organisation (WHO), BioInfoSummer 2013, University of Medicines Programme seminar series, Dr Claudio Mastronardi Adelaide, Adelaide, SA Geneva, Switzerland Encapsulated cell therapies for insulin- The state of reproducible computation in Knowledge discovery from pathology dependent diabetes: What role the stem genomic biology data via machine-learning to enhance cell? th Invited Keynote: Maths of Planet Earth, laboratory diagnosis and provide The 4 Bootes Course on Translational Australian Mathematical Sciences an alternative to animal models in Medicine: The Pathway from Discovery Institute, Melbourne, VIC fundamental research and toxicology to Healthcare, The John Curtin School of Medical Research, The Australian National rd 3 International Conference on University, Canberra, ACT Mr Cameron Jack Proteomics & Bioinformatics, Philadelphia, PA, USA Biology of Systemic Inflammatory Introductory Python Response Syndrome (SIRS) Virus-human interactions demonstrated th The John Curtin School of Medical The 4 Bootes Course on Translational by Ross River virus illustrate a molecular Medicine: The Pathway from Discovery Research, The Australian National basis for pathogen spread, and guides University, Canberra, ACT to Healthcare, The John Curtin School of mathematical insights for the early Medical Research, The Australian National detection of bio-invasion threats University, Canberra, ACT Dr Ben Kaehler Maths for Planet Earth (MPE) BioInvasion Estimating Genetic Distances Using Non- and BioSecurity Workshop, CSIRO Dr Peter Milburn Stationary Processes Discovery Centre, Canberra, ACT Investingating the enteric microbiome Non-animal alternatives for biomedical Genetics Society of Australasia of Native Australian Lizards with Roche research Conference, The University of New South 454 FLX 16S rRNA Gene Amplicon Wales, Randwick, NSW Introduction to Animal Research Workshop, Sequencing University of Sydney, Sydney, NSW Estimating Genetic Distances Using Non- Advances in Genome Biology, Miami, FL, Stationary Processes In silico pattern recognition as a basis USA TIC Presentation, The John Curtin School for animal replacement alternatives in of Medical Research, The Australian fundamental biomedical research National University, Canberra, ACT CReST Seminar series, University of Canberra, Bruce, ACT
Annual Review 2013 113 Dr Maxim Nekrasov Dr Ian Parish Dr Åsa Pérez-Bercoff Different strategies of chromatin Effector T cells self-regulate during Function and evolution of genes in the remodelling at the transcription start sites chronic viral infection by IL-10 over- human protein interaction network Practical Workshop on High-Throughput production” TIC Presentation, The John Curtin School Sequencing Data Analysis, Okinawa OIST, Centre for Biodiscovery & Molecular of Medical Research, The Australian Japan Development of Therapeutics, James National University, Canberra, ACT Cook University, Cairns, QLD Understanding virulence in fungal Professor Chris R Parish Blimp-1 triggers the formation of pathogens immunoregulatory IL-10 producing Th1 Australian Mycology Society (AMS) Joint New insights into Type 1 diabetes cells during chronic viral infection development and therapy Conference, Adelaide, SA 15th International Congress of th Understanding virulence in fungal 15 International Congress of Immunology, Milan, Italy Immunology, Milan, Italy pathogens Multifocal defects in transcription factor Carbohydrate-based therapeutic as anti- Genetics Society AustralAsia (GSA) expression occur during peripheral CD8+ conference, Sydney, NSW cancer drugs T cell tolerance
Lowy Cancer Symposium: Discovering rd 43 Annual Scientific Meeting, Professor Thomas Preiss Cancer Therapeutics, The Lowy Cancer Australasian Society for Immunology, Research Center, The University of New Wellington, New Zealand Principles of gene regulation at the RNA level South Wales, Randwick, NSW EMBL Australia PhD program, Walter and Novel role for heparan sulfate in thymic Dr Hardip Patel Eliza Hall Institute of Medical Research, development of CD8+ T cells Melbourne, VIC 14th Frank and Bobby Fenner Conference Mechanisms of dosage compensation and Z inactivation in snakes RBP atlas: an exploration of interactions - Perspectives on Immune Recognition, between mRNA and proteins and their The John Curtin School of Medical Genetics Society of AustralAsia Annual impact on cardiomyocyte biology Research, The Australian National Meeting, Sydney, NSW ComBio 2013, Perth, WA University, Canberra, ACT Dosage compensation and Z inactivation Translation from scientific discovery to in snakes Gene regulation and the discovery of RNAmerica clinical application Genome Biodiversity Conference, ACT Annual Scientific Meeting of the Canberra, ACT University of Canberra, Bruce, ACT College of Surgeons, Canberra, ACT microRNA changes en route to distinct New insights into Type 1 diabetes Dr Gilberto Paz-Filho cellular states of induced pluripotency development and therapy 36th Annual Meeting of the Molecular Molecular pathways involved in the Biology Society of Japan, Kobe, Japan James Cook University, Cairns, QLD improvement of nonalcoholic fatty liver Heparan sulfate: A multifunctional disease polysaccharide with considerable International Endocrine Scholars Oral Professor Jan Provis therapeutic potential th Session, 95 Annual Meeting of The The macula, the fovea and bloody supply: Institute for Glycomics, Griffith University, Endocrine Society, San Francisco, CA, USA What do we know and how does it help? Gold Coast, QLD Overview of Translational Medicine Ida Mann Plenary Lecture: 45th Royal Cancer: Is a vaccine possible? The 4th Bootes Course on Translational Australian and New Zealand College of The 3rd Annual John Curtin Lecture, The Medicine: The Pathway from Discovery Ophthalmologists Congress, Hobart, TAS John Curtin School of Medical Research, to Healthcare, The John Curtin School of Using light to model and manage AMD The Australian National University, Medical Research, The Australian National 6th Asia and Oceania Conference on Canberra, ACT University, Canberra, ACT Photobiology, Sydney, NSW The macular, vascular patterning and the fovea The Bosch Institute Annual Scientific Meeting on Neuroscience: Modern Challenges and Australian Pioneers, University of Sydney, Sydney, NSW
114 The John Curtin School of Medical Research Dr Charani Ranasinghe Dr Katrina L Randall Dr Charmaine J Simeonovic Mucosal HIV-1 vaccines A missense variant of RasGRP1 increases Islet heparan sulfate is a critical biomarker Australian Institute of Medical Scientists tonic mTOR signaling resulting in aberrant for Type 1 and Type 2 diabetes NSW South West Division Annual accumulation of helper T cells and Australian Institute of Medical Scientists Conference, Canberra, ACT production of autoantibodies – NSW South West Division Annual th HIV-1 pox viral-based vaccination: The 5 International conference on B cells and Conference, Canberra, ACT role of IL-10 vs IL-13 transient inhibition in Autoimmunity, Como, Italy Cell migration and inflammation in Type 1 modulating CD8 T cell immunity DOCK8 is critical for the survival and diabetes: a critical role for heparanase Australian Centre for Hepatitis and HIV function of NKT cells 31st Annual Scientific Meeting, Virology Research Conference, Blue 43rd Annual Scientific Meeting, Transplantation Society of Australia and Mountains, NSW Australasian Society for Immunology, New Zealand, Canberra, ACT Novel vaccine strategies to enhance Wellington, New Zealand Cell migration and inflammation in Type 1 protective mucosal immunity against HIV diabetes: a critical role for heparanase The 4th Bootes Course on Translational Dr Joanne Reed Benaroya Research Institute, Seattle, WA, Medicine: The Pathway from Discovery CR3 Negatively Regulates Toll-like USA to Healthcare, The John Curtin School of Receptor Signaling via the Degradation of Islet isolation results in heparanase- Medical Research, The Australian National MyD88 independent loss of beta cell heparan University, Canberra, ACT ACT and NSW Australasian Society of sulfate (HS) and increased susceptibility How do novel IL-13Ra2 adjuvented Immunology Annual Branch Retreat, to oxidant-mediated damage vaccines modulate HIV-specific mucosal Bowral, NSW 5th International Meeting of the Islet immunity, CD8 T cell avidity and Society, Vancouver, BC, Canada protective immunity Loss of beta cell heparan sulfate th Dr Matthew Rutar 16 International Congress of Mucosal (HS) during islet isolation increases Macrophages and microglia in AMD Immunology, Vancouver, BC, Canada susceptibility to oxidant-mediated IL-4 and IL-13 regulates CD8 co-receptor Panellist: Association for Research in damage Vision and Ophthalmology, Special expression to dampen the quality of anti- Annual Scientific Meeting of the Australian Interest Group (SIG) Seattle, WA, USA viral CD8+ T cell immunity following HIV-1 Diabetes Society and the Australian prime-boost Diabetes Educators Association, Sydney, 15th International Congress of Immunology Dr Faran Sabeti NSW Milan, Italy Diagnostic accuracy of multifocal Islet isolation results in loss of beta HIV-1 IL-13Ra2 adjuvanted vaccines pupillographic objective perimetry in the cell heparin sulfate (HS) and increased modulate antigen-presenting cell subsets age-related eye disease study cohort susceptibility to oxidant-mediated at the lung mucosae and induce high damage World Ophthalmology Congress 2013, avidity HIV-specific CD8 T cells Tokyo, Japan 14th World Congress of the International 43rd Annual Scientific Meeting, Pancreas and Islet Transplant Association, Australasian Society for Immunology, Monterey, CA, USA Wellington, New Zealand Heparan sulfate (HS) deficiency in isolated islets is due to oxidative damage and not heparanase (Hpse)-mediated degradation Immunology of Diabetes Society 13th International Congress, Lorne, VIC Heparan sulfate (HS ) status of human islet beta cells is a sensitive marker of beta cell integrity Immunology of Diabetes Society 13th International Congress, Lorne, VIC Type 1 diabetes: Discovery of a new therapy The Australian National University Innovation Showcase, Sydney, NSW
Annual Review 2013 115 Dr Tatiana Soboleva Dr Rebecca Sweet Professor Carola Vinuesa A new function for histone variants in the B cell extrinsic MyD88 and FcR common Regulation of Tfh cell-mediated selection tissue-specific regulation of pre-mRNA gamma chain control contraction of the in germinal centers splicing autoreactive B cell response Keystone Symposium: B Cell The Annual Scientific Meeting of the Lorne Conference on Infection and Development and Function, Keystone, Endocrine Society of Australia and the Immunity, Lorne, VIC CO, USA Society for Reproductive Biology, Sydney, NSW Control of Tfh Cells Professor David Tremethick Keystone Symposium: on Advances Dr Zan-Min Song H2A.Z inheritance during the cell cycle in the Knowledge and Treatment of and its impact on promoter organisation Autoimmunity, Whistler, BC, Canada Reduced neural proliferation and and dynamics Selection of memory B cells by T cells increased cell death in the hippocampus and dentate gyrus of a rat model of Gordon Conference on Chromatin Department Biochemistry and Molecular Hirschsprung’s disease 3rd Structure and Function, Lucca (Barga), Biology, Monash University, Clayton, VIC Italy International Neural Regeneration Novel layers of regulation of Tfh cell Symposium, Shengyang, China H2A.Z inheritance during the cell cycle biology and pathology and its impact on promoter organisation Intracranial imaging of the spotting lethal The 78th Cold Spring Harbor Laboratory and dynamics rat using Micro CT scanning Symposium on Quantitative Biology 26th International Symposium of Pediatric Cold Spring Harbor Laboratories meeting Immunity and Tolerance, Cold Spring Surgical Research, Cape Town, South on Epigenetics and Chromatin, Cold Harbor, NY, USA Spring Harbor, NY, USA Africa Invited speaker A unique H2A histone variant occupies Seminar Program, The University of the transcriptional start site of active Professor Greg Stuart Adelaide, Adelaide, SA genes Single neuron computation Regulation of Tfh cells Lorne Genome Conference, Lorne, VIC Telluride Neuromorphic Cognition T Follicular Helper Cell Gordon The link between chromatin structure and Engineering Workshop, Telluride, CO, USA Conference, Chinese University of Hong function during early development Dendritic spines Kong, China, Peter Macallum Cancer Institute, Leaders in Science and Medicine Seminar Marine Biology Laboratory, Woods Hole, Melbourne, VIC MA, USA Garvan Institute, Sydney, NSW The link between chromatin structure and Synaptic integration in binocular visual function during early development How T cells control the quality of antibody cortex responses Department of Biochemistry & Molecular Howard Hughes Medical Research Biology, Monash University, CLayton, VIC 4th International Symposium Regulators of Institute, Janelia Farm, VA, USA Adaptive Immunity, Erlangen, Germany, The role of histone variants in regulating Session Chair differentiation and development Regulation of T cell help for B cells Gordon Conference: Inhibition in the Murdoch Children’s Medical Research First Argentinean Spring Course on Central Nervous System, Les Diablerets, Institute, Melbourne, VIC Advanced Immunology, Los Cocos, Switzerland Cordoba Province, Argentina Novel mechanisms controlling mouse and human follicular T cell homeostasis 43rd Annual Scientific Meeting, Australasian Society for Immunology, Wellington, New Zealand
116 The John Curtin School of Medical Research STUDENT PRESENTATIONS
S Achuthan and AC T Bock N Fernando, G Kotwal and K Blackburn The impact of SK channels on dendritic Valter Targeting glucose metabolism - a strategy spikes in cortical L5 pyramidal neurons The protective effect of VCP against light- for overcoming drug resistance in breast Gordon Research Seminar and induced retinal degeneration cancer Conference on Dendrites: Molecules, 3rd International Conference on Clinical 25th Lorne Cancer Conference, Lorne, VIC Structure and Function, Les Diablerts, and Experimental Ophthalmology, Switzerland Chicago, IL, USA R Albarracin The protective effect of the complement D Chaston inhibitor VCP against light-induced retinal Therapeutic potential of 670 nm light in degeneration retinal disorders Expression of dysfunctional connexin40 in vascular endothelium enhances The Australian Society for Medical rd 3 International Conference on Clinical & myogenic constriction and reduces artery Research (ASMR, ACT Branch) New Experimental Ophthalmology, Chicago, distensibility Investigator’s Forum, Canberra, ACT IL, USA Experimental Biology, Boston, MA, USA N Fernando, D Garnett, H Bergmann T Chen, Y Zhu and GA G Kotwal and K Valter B-cell survival, surface BCR and BAFF-R Huttley expression and CD74 metabolism require VCP as a potential treatment for the the intramembrane endopeptidase Do nucleosome-flanking sequences retina: evidence from an AMD animal SPPL2A influence the recruitment of specific model th 2nd IFReC-SIgN Winter School on histone variants? 15 International Congress of Advanced Immunology, Sentosa Island, “Science Passport” presentation, The Immunology, Milan, Italy Singapore John Curtin School of Medical Research, Intramembrane endopeptidase The Australian National University, A Go, MS To, C Stricker, SPPL2A regulates MHC class II antigen Canberra, ACT S Redman, HA Bachor, G Presentation Pathway Do nucleosome-flanking sequences Stuart and VR Daria ACT and NSW Australasian Society of influence the recruitment of specific Immunology Annual Branch Retreat, histone variants? Four-dimensional photostimulation: Bowral, NSW The Australian Society for Medical Stimulating neurons in space and time B-cell survival, surface BCR and BAFF-R Research (ASMR, ACT Branch) New Janelia Farm Shaping the Waves expression and CD74 metabolism require Investigator’s Forum, Canberra, ACT Conference, Ashburn, VA, USA the intramembrane endopeptidase A new alignment algorithm for motif Four-dimensional photostimulation: SPPL2A discovery (MIRE) Stimulating neurons in space and time 43nd Annual Scientific Meeting, Techniques in Computational Genomics Society for Neuroscience Annual Meeting, Australasian Society for Immunology, seminar series, The John Curtin School of San Diego, CA, USA Wellington, New Zealand Medical Research, The Australian National Four-dimensional photostimulation for University, Canberra, ACT studying dendritic integration S Biswas-Legrand, Gordon Research Seminar and M Madigan and K Valter FJ Choong Conference on Dendrites: Molecules, Structure and Function, Les Diablerts, Development of an in vitro human Muller Roles for heparan sulfate and heparanase Switzerland cell model to mimic gliosis in response in islet transplantation to retinal detachment: a platform to 14th World Congress of International Simultaneous 3D multisite investigate the effects of 670nm red light Pancreas and Islet Transplantation photostimulation with high-speed treatment Association (IPITA) Monterey, CA, USA switching The Australian Society for Medical Heparan sulfate and heparanase: European Conference on Biomedical Research (ASMR, ACT Branch) New Biomarkers of islet integrity and damage Optics (ECBO), Munich, Germany Investigator’s Forum, Canberra, ACT in islet transplantation 3D multi-site photostimulation with high- The Transplantation Society of Australia speed switching and New Zealand (TSANZ), Annual 33rd Annual Meeting of the Australasian Scientific Meeting, Canberra, ACT Neuroscience Society, Melbourne, VIC
Annual Review 2013 117 BP Gang, PJ Hogg, PJ Dilda D (Di) Hu, S Zhu and J Potas M Kolic, AYH Chain, AC and AC Blackburn The effect of 670nm light treatment on James, T Maddess and CF Dichloroacetate sensitizes cancer cells recovery of spinal cord injury Carle th towards apoptosis 4 Australian Neurotrauma Symposium, Structure and function in multifocal Keystone symposium: Tumor Metabolism, Hobart, TAS pupillographic objective perimetry Keystone, CO, USA (mfPOP) H Huang Association for Research in Vision and F Haque, F Maclean, D Functional differences between two Ophthalmology, Seattle, WA, USA Nisbet and J Potas classes of layer 2 principal neurons in the piriform cortex in vivo M Kolic, AC James, R Essex, In vivo macrophage polarisation using rd electrospun nanofibres functionalised with 33 Annual Meeting of the Australasian T Maddess and CF Carle Neuroscience Society, Melbourne, VIC interleukin-10 in the peripheral nervous The effect of iris colour on pupil response system Differential odour-processing by two with multifocal pupil objective perimeter 4th Australian Neurotrauma Symposium, classes of principal neurons in the piriform cortex in vivo Australasian Ophthalmology and Vision Hobart, TAS Science Meeting, Hobart, TAS Society for Neuroscience Annual Meeting, San Diego, CA, USA D (Daniel) Hu Y Mei, L Tze, A Enders, EM Odour-processing by principal neurons in Increased mTOR activity and naïve-to- the piriform cortex in vivo Bertram and CC Goodnow memory cell conversion in Rasgrp1Anaef CD4+ T cells Sensory Neuroscience Symposium, The CD8 T cell deficiency in ‘Duan’ mice University of Western Sydney, ACT and NSW Australasian Society of ACT and NSW Australasian Society of Campbelltown, NSW Immunology Annual Branch Retreat, Immunology Annual Branch Retreat, Bowral, NSW Differential odour-processing by two Bowral, NSW classes of principal neurons in the piriform cortex in vivo D (Di) Hu and J Potas A Mishra, AC Blackburn, The Australian Society for Medical C Parish, M-L Wong and Effect of 670 nm photobiomodulation on Research (ASMR, ACT Branch) New Spinal Cord Injury Investigator’s Forum, Canberra, ACT J Licinio Canberra Health and Research Meeting Leptin increases metastatic potential of (CHARM), Canberra Hospital, Canberra, Y Jeelall breast cancer cells ACT Investigating the effects of lymphoma AACR Tumor Invasion and Metastasis Effect of 670 nm photobiomodulation on Conference, San Diego, CA, USA mutations enhancing NFKB signalling spinal cord injury Lorne Cancer Conference, Lorne, VIC Kioloa at Newcastle Neuroscience Colloquium, Newcastle, NSW Investigating the potential overlapping pathogenesis of autoimmunity and cancer D (Di) Hu, J Potas and S Zhu Australian Society for Medical Research New Investigator Forum, Canberra, ACT Effects of 670 nm photobiomodulation on Investigating the potential overlapping recovery following hemi-contusion spinal pathogenesis of autoimmunity and cancer cord injury Canberra Health Annual Research XXXVII Annual Meeting of the Brazilian Meeting (CHARM), Canberra, ACT Society of Neuroscience and Behaviour Belo Horizonte, MG - Brazil
118 The John Curtin School of Medical Research S Morton M Ratnadiwakara, R JWG Seamons, A Bubna- Impairment of endothelial Connexin40 Williams, M Rooke and AC Litic, MS Barbosa and T causes hypertension in mice via an EDH- Blackburn Maddess independent pathway Vitamin D metabolism, PTH and breast Isotrigon texture discrimination by a small Joint meeting of Australian Vascular Biology cancer susceptibility in mice number of underlying neural mechnisms Society (AVBS) and Australian and New Zealand Microcirculatory Society (ANZMS), European Conference on Cancer Australasian Ophthalmology and Vision Barossa Valley, SA Genomics, Heidelburg, Germany Science Meeting, Hobart, TAS Interference with endothelial gap junctional coupling via Connexin40 produces J Robertson T Sibbritt, BJ Parker, DT hypertension in mice Electrical stimulation alters patterns of Humphreys, HR Patel, AL Canberra Health and Research Meeting epileptiform activity in the piriform cortex Statham, SJ Clark and T Preiss (CHARM), Canberra Hospital, Canberra, ACT The Australian Society for Medical The association of RNA cytosine Now you see it, now you don’t: Switchable Research (ASMR, ACT Branch) New methylation with post-transcriptional Transgenic Mice Investigator’s Forum, Canberra, ACT regulation and cancer Canberra Health and Research Meeting RNA Network of Australasia: RNA Society (CHARM), Canberra Hospital, Canberra, ACT Z Sabouri, K Horikawa, K Seminar, Research School of Biology, The Now you see it, now you don’t: Switchable Randall, R Brink and CC Australian National University, Canberra, Transgenic Mice Goodnow ACT The Australian Society for Medical Research (ASMR, ACT Branch) New Investigator’s Antibody redemption: removal of self- T Sibbritt, BJ Parker, HR Forum, Canberra, ACT reactivity from IgD+ anergic B cells by purifying selection in germinal centers Patel, DT Humphreys, JE Interference with endothelial gap junctional Squires, NJ Beveridge, SJ coupling via Connexin40 produces Duke Human Vaccine Institute, Duke hypertension in mice University, Durham, NC, USA Clark and T Preiss The Australian Society for Medical Research Antibody redemption: removal of self- RNA methylation: a mechanism for post- (ASMR, ACT Branch) New Investigator’s reactivity from IgD+ anergic B cells by transcriptional control that is deregulated Forum, Canberra, ACT purifying selection in germinal centers in cancer? University of California San Diego, San 18th Annual Meeting of the RNA Society, A Pratama Diego, CA, USA Davos, Switzerland Antibody redemption: removal of self- MicroRNA-146a represses Tfh cell reactivity from IgD+ anergic B cells by T Sibbritt, HR Patel, DT program purifying selection in germinal centers Humphreys, JE Squires, SJ ACT and NSW Australasian Society of Vaccine Research Center of the National Immunology Annual Branch Retreat, Institutes of Health, Washington DC, USA Clark and T Preiss Bowral, NSW Antibody redemption: removal of self- Investigating the role of RNA methylation reactivity from IgD+ anergic B cells by in post-transcriptional regulation using a T Racic and K Valter purifying selection in germinal centers prostate cancer cell model Can 670nm red light treatment ameliorate Keystone Symposium: B Cell 34th Annual Lorne Genome Conference, damage following retinal detachment? Development and Function, Keystone, Mantra Lorne, Lorne, VIC Observations from a rodent model CO, USA Australasian Ophthalmology and Vision Science Meeting, Hobart, TAS S Sane Wavefront correction for optimised light M Ratnadiwakara, R delivery into brain tissue Williams and AC Blackburn The ANZCOP (Australian and New Vitamin D, parathyroid hormone, Cyp2r1 Zealand Conference on Optics and and breast cancer susceptibility in mice Photonics) Conference, Fremantle, WA American Association for Cancer Research: Breast Cancer Research Conference, San Diego, CA, USA
Annual Review 2013 119 M Singh JQ Wang, Y Jeelall, K M Yabas Tracking and identifying autoimmune- Horikawa, and CC Goodnow ATP11C controls early B cell development inducing T cell clones in Aire-/-Cblb-/- MYD88 mutations in B cells – effects on but not their function mice NFkappaB activity, B cell survival and University of Chicago, Chicago, IL, USA rd proliferation 43 Annual Scientific Meeting, ATP11C controls early B cell development Australasian Society for Immunology, Lorne Cancer Conference, Lorne, VIC by blocking pre-BCR signaling Wellington, New Zealand University of Texas Southwestern Medical Tracking and identifying autoimmune- DK Wijesundara, RJ Center, Dallas, TX, USA inducing T cell clones in Aire-/-Cblb-/- mice Jackson, DC Tscharke and ATP11C controls early B cell development Keystone Symposium: Advances but not their function in the Knowledge and Treatment of C Ranasinghe Keystone Symposium: B Cell Autoimmunity, Whistler, BC, Canada IL-4 and IL-13 regulate CD8 co-receptor Development and Function, Keystone, expression to dampen the quality of anti- Tracking and identifying autoimmune- CO, USA inducing T cell clones in Aire-/-Cblb-/- mice viral CD8+ T cell immunity following HIV-1 prime-boost vaccination Deficiency of the P4-type ATPase RCAI International Summer Program, ATP11C in mice causes stomatocyte 15th International Congress of Yokohama, Japan formation and anemia due to a shortened Immunology, Milan, Italy erythrocyte lifespan A Theodoratos, JE Canberra Health Annual Research DK Wijesundara, DC Dahlstrom, R Galgamuwa, Meeting, Canberra, ACT Tscharke, RJ Jackson, and JA Smiles, L Tian, AC The putative aminophospholipid C Ranasinghe translocase ATP11C controls early B-cell Blackburn and PG Board development but not their function Dynamic regulation of IL-4 receptor alpha Prevention of cisplatin-induced rd (IL-4Ra) following viral infections and 43 Annual Scientific Meeting, nephrotoxicity by dichloroacetate HIV-1 vaccination correlates with anti-viral Australasian Society for Immunology, 10th International ISSX (International CD8+ T cell quality Wellington, New Zealand Society for the Study of Xenobiotics) Keystone Symposium: HIV Vaccines (X2), Meeting, Toronto, ON, Canada Keystone, CO, USA H Yang Characterization of the interactions S Trivedi, R Jackson, L between RNA and ‘moonlighting’ Sedger, J Stambas and C metabolic enzymes in rodent Ranasinghe cardiomyocytes and their change in response to stress Intranasal delivery of recombinant HIV- The Australian Society for Medical 1 fowl poxvirus vaccines can induce Research (ASMR, ACT Branch) New excellent high avidity CD8 T immunity by Investigator’s Forum, Canberra, ACT recruiting unique antigen presenting cell subsets to the lung mucosae 16th International Congress of Mucosal JY Yap Immunology, Vancouver, BC, Canada Thymic epithelial cells can delete the majority of strongly self-reactive CD4+ J Vélez thymocytes in the natural TCR repertoire ACT and NSW Australasian Society of Translational genomics of complex traits: Immunology Annual Branch Retreat, Attention Deficit/Hyperactivity Disorder Bowral, NSW and Alzheimer’s disease Program in Human Genetics, University of Chile School of Medicine, Santiago, Chile
120 The John Curtin School of Medical Research COMMUNITY
Ms Nur Diana Anuar Associate Professor Dr Anneke Blackburn Member: Australia-Malaysia Muslim John M Bekkers Coordinator: Cancer Biology Forum, Family Association (AMMF) Director: Australian Course in Advanced JCSMR, ANU, Canberra, ACT Member: Malaysian Postgraduate Neuroscience (ACAN), Moreton Bay Presenter and Host: JCSMR Outreach Student Association (MyPSA) Research Station, North Stradbroke Program, Canberra, ACT Island, QLD Organizing committee member: MyPSA Member: American Association for for GAZA Fundraiser, Canberra, ACT Reviewing Editor: Neuroscience Letters; Cancer Research Frontiers in Neuroscience; BMC Member: Australian Society for Medical Neuroscience Dr Stuart Archer Research (ASMR) Chair: JCSMR Scholarships Committee Grant-Assessor: NHMRC Member: Kathleen Cunningham Convenor: JCSMR School Seminar Consortium for Research into Familial Reviewer: PLoS One program Breast Cancer (kConFaB) Member: JCSMR Education Committee Invited Speaker: ACT Cancer Council Professor Mauricio Member: ANU Neuroscience Pink Ribbon Breakfast: Targeting cancer Arcos-Burgos Management Committee metabolism with dichloroacetate Editorial Board Member: Molecular Member: Eccles Institute of Neuroscience National Breast Cancer Foundation Genetics and Genomics Medicine Director Search Committee National Research Roadshow: Targeting cancer metabolism with dichloroacetate Editorial Reviewer: Nature Genetics; Council Member: Australasian Molecular Psychiatry; Human Genetics, Neuroscience Society Invited speaker: Zonta Club of Canberra Nephron; Bioinformatics; Translational Breakfast meeting: Targeting cancer Ex officio Member: ACAN Management Psychiatry, Annals of Human Genetics; metabolism with dichloroacetate Committee of the Australasian The ANU Undergraduate Research Neuroscience Society Journal Professor Philip G Board Member: Australasian Neuroscience Society Member: ACT Gene Technology Advisory Ms Bhavani Balakishnan Council Member: Australian Physiological Society Presenter and Host: Academic visit from Awards Committee Member: International Member: Society for Neuroscience US Indiana University Purdue University Society for the Study of Xenobiotics (IUPUI) Member: International Brain Research Editorial Board Member: The Open Drug Organization (IBRO) Workshop Presentation: Missense Metabolism Journal; Drug Metabolism Mutation Project, a gene driven approach Presenter and Participant: National Youth Reviews; The Sirraj Medical Journal for generating mouse models for cancer Science Forum, JCSMR, ANU, Canberra, research, Lorne Cancer Conference, ACT Dr Jean-Didier Breton Lorne, VIC Presenter and Host: JCSMR Outreach Program, Canberra, ACT Chair: Neuroscience Seminar series, JCSMR, ANU, Canberra, ACT Dr Nicole Beard Judge: Australasian Brain Bee Challenge Co-organiser: Annual Neuroscience Presenter and Participant: National Youth Final, Australasian Neuroscience Society, Kioloa Colloquium, ANU Coastal Campus, Science Forum, JCSMR, ANU, Canberra, Gold Coast, QLD Kioloa, NSW ACT Presenter and Participant: Australian Chair: Organising committee: Gage Dr Edward M Bertram Brain Bee Challenge, ACT Regional Conference on Muscle Excitation- Steering Committee Member: Finals, JCSMR, Canberra, ACT Contraction Coupling International Mouse Phenotyping Member: Australasian Neuroscience Secretary: Gage Conference Society Consortium Society Academic Editor: PLoS One Finance Sub-Committee Member: Member: Society for Neuroscience US International Mouse Phenotyping Council Member: Australian Physiological Consortium Member: Federation of European Society Neuroscience Society Participant: Australian Government- Member: Cardiac Society of Australia and European Union 2nd Workshop on Member: French Neuroscience Society New Zealand Research Infrastructure Member: Biophysical Society USA
Annual Review 2013 121 Mr Bob Buckley Dr Geeta Chaudhri Associate Professor Member: The Australasian Genomics Member: American Society Virology Matthew Cook Technologies Association (AMATA) Member: Federation of Clinical Chairman: Royal Australasian College Immunological Societies of Physicians, Joint Specialist Advisory Committee, Immunology and Allergy Dr Corinne Carle Member: Australasian Society for Member: Australasian Neuroscience Immunology Member: Royal Australasian College of Physicians Curriculum committee Society (ANS) Member: International Society for Member: Association for Research in Cytokine and Interferon Research Member: Royal Australasian College of Vision and Ophthalmology Physicians College education committee Committee Member: Australian Society Dr Julian Choy Examiner: Royal College of Pathologists of Australasia (Immunopathology) for Vision Research Presenter and Participant: JCSMR Open Presenter and Participant: JCSMR Open Day, The John Curtin School of Medical Member: NHMRC Grant review panel Day, The John Curtin School of Medical Research, ANU, Canberra, ACT Editorial Board Member: Clinical and Research, ANU, Canberra, ACT Presenter and Participant: National Youth Translational Immunology Presenter and Participant: National Youth Science Forum, JCSMR, ANU, Canberra, Science Forum, JCSMR, ANU, Canberra, ACT Ms Tiffany Cripps ACT Presenter and Participant: Australian Member: The Australasian Genomics Presenter and Participant: Clinical Brain Bee Challenge, ACT Regional Technologies Association (AMATA) Research Suites Open Day, The John Finals, JCSMR, Canberra, ACT Curtin School of Medical Research, ANU, Member: Australasian Neuroscience Dr Stephen Daley Canberra, ACT Society Member: Society for Neuroscience USA ACT Branch Councillor: Australasian Dr Marco G Casarotto Society for Immunology Presenter and Host: JCSMR Outreach Mr Aaron Chuah Editor: News and Commentary section: Program, Canberra, ACT Immunology and Cell Biology Member: The Australasian Genomics Convenor: Celebration of Professor Chris Council Member: Australian Society for Technologies Association (AMATA) Biochemistry and Molecular Biology Parish’s Honorary Life Membership of (ASBMB) the Australasian Society for Immunology, Dr Jennifer Clancy JCSMR, Canberra, ACT Member: Australian Society for Medical Research (ASMR) Member: Australian Society for Dr Vincent Daria Member: Australian Biophysics Society Biochemistry and Molecular Biology (ABS) Member: Australian Society for Medical Co-Chair: Neurophotonics Session, Member: Biophysical Society US Research European Conferences on Biomedical Optics, Munich, Germany Member: NHMRC Grant Review Panel: Member: RNA Network of Australasia Presenter and Participant: National Youth Biochemistry and Cell Biology Member: JCSMR Operations Committee Science Forum, JCSMR, ANU, Canberra, Grant-Assessor: NHMRC, ARC ACT Reviewer: Circulation Research; PLoS Presenter: Australian Course in Advanced One; microRNA; F1000Research Neuroscience (ACAN), North Stradbroke Island, QLD Editorial Board: ISRN Optics Member: Australasian Neuroscience Society Member: Optical Society of America Member: The International Society for Optics and Photonics Member: Australian Optical Society
122 The John Curtin School of Medical Research Dr Debjani Das Dr Anselm Enders Professor Jill Gready Member: Cognitive Neuroscience Society ACT Branch Councilor: Australasian Presenter and Participant: JCSMR Open Member: International Behavioural and Society of Immunology Day, The John Curtin School of Medical Neural Genetic Society Member: CMBE Research Committee Research, ANU, Canberra, ACT Member: Society for Social Neuroscience Member: RSB Honours Convening Editorial Board Member: Bioinformatics and Biology Insights Member: Australian Society for Psychiatric committee Research Member: Australasian Society for Member: ANU Supercomputing Time Allocation Committee Reviewer: Addiction; Addiction Biology; Immunology PLoS ONE; Journal of Attention Member: European Society for Co-convenor: ANU Photosynthesis Disorders; Psychological Medicine; Immunodeficiencies Initiative Scientific Committee Developmental Psychobiology; Social Fellow: Royal Australian Chemical Institute Psychiatry and Psychiatric Epidemiology Professor Christopher Member: Australian Society for C Goodnow Biochemistry and Molecular Biology Dr Michael Dobbie (ASBMB) Member: Australian Academy of Science Member: Australian Society for Biophysics Exhibition Booth Host: Research Special Elections Committee Infrastructure, Annual Australian Science Communicators National Conference, Medical Science Review Board Member: Dr Rebecca Haddock Sydney, NSW Juvenile Diabetes Research Foundation Member: Infection and Immunity Strategic Panel Member: NHMRC Early Career Fellowships Dr Guowen Duan Advisory Committee, The Wellcome Trust, UK Committee Chair: JCSMR Scholarships Honorary Fellowship: CSIRO Editorial Board Member: Immunity; Committee The Journal of Experimental Medicine; Committee Member: Level A/B Professor Angela F Dulhunty Mammalian Genome; Journal of representative, JCSMR Executive Autoimmunity; BMC Biology Committee Immediate Past President: Australian Society for Biophysics Communicating Editor: International ACT Representative: Australian and New Immunology Zealand Microcirculation Society Organiser: Curtin Conference on Muscle, Canberra, ACT Council Member: EMBL Australia Member: National Association of Research Fellows Member: Australian Physiological Society Member: Australasian Society for Immunology Member: Australian Physiological Society Member of the National Committee for Biomedical Sciences Member: American Association for the Member: Australian Society of Advancement of Science Pharmacologists and Toxicologists Member: ARC College Member: American Association of (Cardiovascular SIG Committee member) Member: Biophysical Society US Immunologists Editorial Board Member: Calcium Binding Proteins; Ion Channels; The Biochemical Journal Grant Assessor: ARC AusReader, NHMRC, European, USA and NZ Granting agencies
Annual Review 2013 123 Ms Angela Higgins Associate Professor Gavin Mr Cameron Jack Member: The Australasian Genomics Huttley Member: The Australasian Genomics Technologies Association (AMATA) Convenor: Techniques in Computational Technologies Association (AMATA) Genomics Seminar Series Professor Caryl E Hill Convenor: Bioinformatics drop-ins Dr Ben Kaehler Guest Reviewing Editor: Journal of Organising committee chair: Workshop Member: Genetics Society of AustralAsia Physiology (London) on Bioinformatics for High Throughput Committee Member: Cardiovascular Sequencing Associate Professor Special Interest Group, Australian Academic Head: Genome Discovery Unit Guna Karupiah Physiological and Pharmacological Chair: Genome Discovery Unit Operations Society Vice-President: Federation of Committee Committee Member: NHMRC Early Immunological Societies of Asia Oceania Member: Genetics Society of AustralAsia Career Researcher Awards Committee (FIMSA) Member: Society for Molecular Biology Presenter and Participant: National Youth Vice-President - Federation of Clinical and Evolution Science Forum, JCSMR, ANU, Canberra, Immunological Societies (FOCIS) ACT Reviewer: PLoS Genetics; PLoS ONE Member: American Association of Committee Member: JCSMR Operations Grant Reviewer: ARC, NHMRC program Immunologists Committee grants Member: American Society for Management Committee Member: Media interviews: The medical Microbiology Histology, Electron Microscopy and significance of snake venom, ABC Radio, Member: American Society Virology AFP, 3AW FACS, JCSMR Member: International Society for Member: JCSMR Workshop Users Editorial Board Member: Frontiers in Cytokine and Interferon Research Evolutionary and Population Genetics; Committee Member: Australasian Society for Biology Direct; PLoS ONE Management Committee Member: ANU Immunology Centre for Advanced Microscopy Member: Australian Society for Medical Member: Australian Physiological Society Ms Carly Hynes Research (ASMR) Member: Australian Neuroscience Society Organiser: NSW miRNA Users Workshop, RNA network of Australasia and ASBMB Member: ANZ Microcirculation Society Professor Trevor D Lamb Reviewer: PLoS ONE Member: American Physiological Society Consulting Editor: Journal of Physiology Member: The RNA Society Member: Society for Neuroscience USA Member: The Australian Society for Dr Yalin Liao Medical Research (ASMR) Dr Lauren Howitt Member: Nutrition Society of Australia Member: Australian Society for Member: Canberra Society of Chinese Presenter and Participant: National Youth Biochemistry and Molecular Biology Scholars Science Forum, JCSMR, ANU, Canberra, (ASBMB) ACT Participant: National Scientists and Member: Australasian Microarray and Mathematicians in Schools Program, in Member: Cardiovascular SIG Committee, Associated Technologies Association association with Canberra Girls Grammar Australasian Society of Clinical and (AMATA) Experimental Pharmacologists and School, Deakin, ACT Member: Golden Key International Toxicologists Honour Society Member: Australian and New Zealand Microcirculation Society
124 The John Curtin School of Medical Research Professor Julio Licinio Member: Society of Biological Psychiatry Associate Professor Review Panel Member: US National Fellow: The Royal Australian and New Brett Lidbury Zealand College of Psychiatrists Institute of Mental Health, National Public lecture: Replacing Animals in Institutes of Health Interventions Co-Chair: ANU College of Medicine, Medical Research - A World View and the Committee for Adult Mood and Anxiety Biology & Environment, Committee for Australian Perspective Disorders (ITMA) Academic Health Science Centre and Manning Clark House, Forrest, ACT Member: United States Department of Translational Research Health and Human Services, Secretary Editor-in Chief: Molecular Psychiatry Dr Fiona Lithander Advisory Committee on Genetics, Health Editor-in Chief: The Pharmacogenomics and Society Member: Year 1 and Year 2 Examination Journal Scientific Program Committee Chair: Committees, ANU Medical School, ANU, Editor-in Chief: Translational Psychiatry American Psychiatric Association Canberra, ACT Editorial Board Member: Australian and Member, Membership and Scientific Member: Phase 1 Committee, ANU New Zealand Journal of Psychiatry Program Committees: American College Medical School of Neuropsychopharmacology Member: National Selection Committee: Council Member: ACT Branch, Nutrition Australian-American Fulbright Course Co-Convener: The Bootes Course Society of Australia Commission on Translational Medicine, JCSMR, Member: Nutrition Society Australia Committee Member: ANU Genome Canberra, ACT Member: Australia New Zealand Obesity Discovery Advisory Committee Member: Scientific Program Committee: Society Member: NHMRC - Promoting & American College of Psychiatrists Member: Nutrition Society, UK & Ireland Maintaining Good Health Expert Working Member: Communications Committee: Group Member: Association for the Study of Association for Clinical Research Training Obesity, UK President: International Society of Founding President: International Society Translational Medicine Registered Nutritionist; Association for of Pharmacogenomics (ISP) Nutrition, UK Member: National Science Colloquium Member: American Society of Human Judge: ASMR New Investigator Forum, Review Panel Member: National Institute Genetics Canberra, ACT for Health Research Biomedical Research Member: Association for Patient-oriented Centres and Biomedical Research Units, Presenter and Participant: Clinical Investigation United Kingdom Research Suites Open Day, The John Member: Collegium Internationale Neuro- Curtin School of Medical Research, ANU, Member: NHMRC Genetics Review Panel Psychopharmacologicum (CINP) Canberra, ACT Organising Committee Member: Sarah Member: Society for Neuroscience USA Grace Sarcoma Foundation Member: The Endocrine Society Dr Fabio Longordo Member: Australasian Society for Member: American Federation for Medical Psychiatry Research Presenter and Participant: JCSMR Open Research Day, The John Curtin School of Medical Editorial Board Member: Translational Research, ANU, Canberra, ACT Medicine: Current Research Journal Member: Australasian Neuroscience Society Member: Swiss Neuroscience Society Member: Society for Neuroscience USA
Annual Review 2013 125 Professor Ted Maddess Professor Klaus I Matthaei Professor Christopher R Director: ARC Centre of Excellence in Deputy Chair: ANU Institutional Parish Vision Science Recombinant DNA Biosafety Committee Medical Research Advisory Committee Section Editor: Clinical and Experimental Reviewer: Royan Institute International Member: The Australian Cancer Research Ophthalmology Research Awards Foundation Session Chair: International Imaging and Reviewer: National Health & Medical Committee Member: Melbourne Perimetry Society meeting Research Council International Congress of Immunology 2016 Bid Chair: Organising Committee: AOVSM Reviewer: AUSReader Australian meeting, Melbourne, VIC Research Council Founding Member: World AllergoOncology Task Force (Vienna- Member: American Academy of Affiliate Member: Centre for the Molecular based) Neurology (AAN) Genetics of Development Member: American Academy of Member: Australian Physiological Society Dr Ian Parish Ophthalmology (AAO) Member: ANZ Microcirculation Society Member: Association for Vision Research Member: Australasian Society for Immunology and Ophthalmology (ARVO) Dr Peter Milburn Member: Imaging and Perimetry Society News and Commentary Editor: (IPS) Member: The Association of Biomolecular Immunology and Cell Biology Resource Facilities (ABRF) (USA) Member: International Society for Clinical Electrophysiology of Vision (ISCEV) Dr Hardip Patel Dr Teresa Neeman Member: Royal Australian & NZ College of Member: The Australasian Genomics Ophthalmology (RANZCO) Member: Safety monitoring committee: Technologies Association (AMATA) Otsuka clinical trial in patients with COPD Member: Optical Society of America Member: Genetics Society of Australasia (OSA), Vision Sciences Society (VSS) Member: Safety monitoring committee for Immunotherapeutics clinical trial of Member: Board of Administration: monoclonal antibody in patients with Dr Gilberto Paz-Filho National Vision Research Institute multiple myeloma (Melbourne) Editorial Board Member: Brazilian Statistical reviewer: Cochrane Archives of Endocrinology and Member: Scientific Advisory Board of Collaborative Acute Respiratory Infections Metabolism EyeCo Pty Ltd Group Member: Latin American Thyroid Society Organising Committee Member: Statistical reviewer: Cochrane International Imaging and Perimetry Member: Brazilian Society of Collaborative Breast Cancer Group Society, Melbourne, VIC Endocrinology and Metabolism Statistical consultant: MS Australia Member: Eccles Institute of Neuroscience Member: The Endocrine Society Director Search Committee Dr Maxim Nekrasov Committee Member: The Endocrine Member: Zinkernagel/Doherty Fellowship Society’s International Endocrine Scholars Selection Committee, JCSMR Workshop host and Presenter: Chromatin Program Task Force techniques in Plants, Monash University, Committee Member: The Endocrine Member: Board of Directors, Australian Melbourne, VIC College of Optometry Society’s Trainee & Career Development Core Committee Member: Board of Administration, National Vision Research Institute (NVRI) Ms Stephanie Palmer Committee Member: The Endocrine Society’s Awards Review Committee Member: NHMRC Assigners Academy Member: The Australasian Genomics Technologies Association (AMATA) External Assessor: National Health and Medical Research Council Project Grants Dr Claudio Mastronardi Organising Committee Member: The Member: Society for Neuroscience USA 3rd Bootes Course on Translational Member: The Endocrine Society Medicine: The Pathway from Discovery to Healthcare, The John Curtin School of Organising Committee Member: Clinical Medical Research, The Australian National Research Suites Open Day, The John University, Canberra, ACT Curtin School of Medical Research, ANU, Canberra, ACT Co-convenor: Masters of Translational Medicine program at The Australian National University, Canberra, ACT
126 The John Curtin School of Medical Research Organising Committee Member and Host: Member: Medical Science Theme CAP committee/ (PhD) thesis examiner: Clinical Research Suites Open Day, The Committee, ANU Medical School, The UNSW, University of Sydney, University John Curtin School of Medical Research, Australian National University, Canberra, of Newcastle, UQ, UWA, University ANU, Canberra, ACT ACT of Adelaide, Swiss Federal Institute of Member: Professional Behavioural Technology Zurich, Centre de Regulació Dr Kaiman Peng Committee, ANU Medical School, The Genòmica Barcelona (Spain) Australian National University, Canberra, Editorial Board Member: PLoS ONE Member: The Australasian Genomics ACT Technologies Association (AMATA) Reviewer: Cell; Science; Nat Cell Biol; Member: Honours Neuroscience PLoS Biol; EMBO J; TIBS; Genome Biol; Committee, JCSMR, ANU NAR; RNA Ms Eva Pillai Representative: Brazilian Higher Equity Officer: ANU Postgraduate and Education Delegation to Australia, ANU Mrs Anne Prins Research Student Association (PARSA) Medical School, ANU, Canberra, ACT Presenter and Participant: National Youth PARSA Representative: ANU College of Science Forum, JCSMR, ANU, Canberra, Medicine, Biology and the Environment Professor Thomas Preiss ACT on the Postgraduate Representative Council Member: Australian Society for Presenter and Host: JCSMR Outreach Biochemistry and Molecular Biology Program, Canberra, ACT Postgraduate Representative: ANU Education Committee, HDR Sub- Chair: RNA network Australasia, an Presenter and Co-organiser: Surgical Cut committee ASBMB SIG Up Workshops, CIT, Bruce, ACT Member: ANU Access and Equity Member: Australasian Microarray & Presenter and Co-organiser: Surgical Cut Committee Associated Technologies Association Up Workshops, Launceston, TAS Senior Residential Scholar: Toad Hall, Member: German Society for Demonstrator: Anatomical Pathology, CIT Postgraduate Residence Biochemistry and Molecular Biology and University of Canberra, Bruce, ACT Board Member: Lorne Genome Inc Dr Jason Potas Member: Australian Genome Conference Professor Jan Provis Organising Committee 2012, Lorne, VIC Member: Australasian Neuroscience Member: Scientific Advisory of the Society Symposium co-chair: ComBio Ophthalmic Research Institute of Australia Conference 2012, Adelaide, SA (ORIA) Member: Brazilian Society of Neuroscience and Behaviour SBNeC ANU Representative: University round Chair: Research Grants Advisory Group, table of Research Australia Retina Australia Presenter and Participant: JCSMR Open Day, The John Curtin School of Medical Deputy Chair: Research Committee Associate Director: ARC Centre of Research, ANU, Canberra, ACT CMBE/ANU Excellence in Vision Science Presenter and Participant: Australian Member: ANU Major Equipment Member: Scientific Review of Progress – Brain Bee Challenge, ACT Regional Committee ClemCell Project, Bond University Finals, JCSMR, Canberra, ACT Member: JCSMR External Relations Member: Bendigo Bank Scholarship Presenter and Participant: National Youth Committee Advisory Group, Braidwood-Bungendore District. These scholarships are for Science Forum, JCSMR, ANU, Canberra, Member: JCSMR Internal Grant Review students entering tertiary education and ACT Committee are in need of and eligible for financial Member: Year 1 and Year 2 Examination Member: JCSMR Group Leaders’ Forum support. Committees, ANU Medical School, The Australian National University, Canberra, Grant-Assessor: NHMRC, ARC, National ACT Heart Foundation, Clive and Vera Mr Roy Ramiscal Ramaciotti Foundation, Sylvia and Charles Member: Phase 1 Curriculum Committee, Viertel Foundation, Cure Cancer Australia Member: Australasian Society for ANU Medical School, The Australian Foundation, Children’s, Youth and Immunology National University, Canberra, ACT Women’s Health Service, South Australia, Member: JCSMR Education Committee Marsden Fund (NZ), Université de Liège Member: Phase 1 Assessment Member: JCSMR Social Committee Committee, ANU Medical School, The Concerted Research Actions (Belgium), Australian National University, Canberra, Swiss National Science Foundation, ACT Austrian Science Fund (FWF), Cancer Research UK, Wellcome Trust UK, NSF (USA), Foundation for Polish Science, French National Research Agency (ANR), and European Research Council
Annual Review 2013 127 Dr Charani Ranasinghe Dr Charmaine Simeonovic Associate Professor Member and Volunteer worker: Editorial Board Member: Cell Christian Stricker Belconnen Arts Centre Transplantation Chair: Medical Science Committee, ANU Member and Volunteer worker: AIDS Member: The Australasian Society for Medical School, ANU, Canberra, ACT Action Council ACT Immunology Chair: Year 2 Examination Committee, Member: Australasian Society for Member: The Transplantation Society of ANU Medical School, ANU, Canberra, Immunology Australia and New Zealand. ACT Member: Society for Mucosal Member: The Transplantation Society Presenter and Participant: National Youth Immunology (International) Science Forum, JCSMR, ANU, Canberra, ACT ACT representative: ASI Mucosal Member: The International Pancreas and Immunology Special Interest Group Islet Transplant Association Presenter and Participant: Indigenous Day, ANU Medical School, ANU, Editorial Board: Journal of Sexually Member: The Immunology of Diabetes Canberra, ACT Transmitted Diseases Society (International) Reference Group Member: Independent Organising Committee: Australian Society Member: The International Management Group, National Baboon for HIV Medicine (ASHM) Conference, Xenotransplantation Association Colony, Camperdown, NSW Melbourne, VIC Member: The Cell Transplantation Society Member: ANU Neuroscience Member: The Australian Diabetes Society Dr Danny Rangasamy Management Committee Member: Australasian Neuroscience Grant Assessor: NHMRC Mr Geoff Sjollema Society Associate Editor: Biotechnology Letters Workshop Presentation: Missense Member: Society for Neuroscience USA Member: Australian Society for Mutation Project, a gene driven approach Biochemistry and Molecular Biology for generating mouse models for cancer Professor Greg J Stuart (ASBMB) research, Lorne Cancer Conference, Lorne, VIC Instructor and presenter: Australian Ms Belinda Ryan Course in Advanced Neuroscience Dr Tanya Soboleva (ACAN), North Stradbroke Island, QLD Member: ASBMB Convenor: Australian Brain Bee Participant: Scientists in Schools Challenge, ACT Regional Finals, JCSMR, Ms Tennille Sibbritt program, in association with Telopea Park Canberra, ACT School, Barton, ACT Member: Australian Epigenetic Alliance Organiser: Kioloa Neuroscience Member: Society of Reproductive Biology Colloquium, ANU Coastal Campus, Member: Australian Society for Kioloa, NSW Biochemistry and Molecular Biology Dr Zan-Ming Song (ASBMB) Associate Editor: Frontiers in Cellular Neuroscience Member: Golden Key International Editorial: Journal of Community Medicine Honour Society and Health Education Advisory Editorial Board: Trends in Editorial: Scientific World Journal Neurosciences Member: The RNA Society Member of Council: Sydney Chapter of the US Society of Neuroscience Member: Australasian Neuroscience Society Member: Australian Academy of Science Member: Society for Neuroscience USA Member: Australian Neuroscience Society Symposium Programming and Advisory Group (SPAG)
128 The John Curtin School of Medical Research Dr Norimitsu Suzuki Member: International Society of Ocular Member: Education Committee, JCSMR Cell Biology (ISOCB) Presenter and Participant: National Youth Member: Postdoc selection committee, Science Forum, JCSMR, ANU, Canberra, Member: Australasian Neuroscience JCSMR Department of Genome Biology, ACT Society Computational Genomics Group Member: Australasian Neuroscience Member of Australia and New Zealand Member: Animal Services Review Society Association for Clinical Anatomists Committee (ANZACA) Member: Society for Neuroscience USA Member: Zinkernagel/Doherty Fellowship Member: CUBE NET Selection Committee, JCSMR Dr Rebecca Sweet Presenter and Participant: National Youth Member: Curriculum Planning Day, Science Forum, JCSMR, ANU, Canberra, Academic Unit of Psychiatry & Addiction Presenter and Participant: JCSMR Open ACT Medicine, ANU Medical School Day, The John Curtin School of Medical Participant: Scientists and Member: American Association for Research, ANU, Canberra, ACT Mathematicians in Schools Advancement of Science Member: Australasian Society for Member: American College of Immunology Mr Jorge Vélez Neuropsychopharmacology Member: American Psychiatric Professor David Tremethick Member: Grupo de Neurociencias, University of Antioquia, Medellín, Association Committee Member: ACT branch of the Colombia Member: International Society for Australian Society for Medical Research Pharmacogenomics ACT representative: The Australian Professor Carola Vinuesa Member: The Endocrine Society Society of Biochemistry and Molecular Member: Society for Neuroscience USA Biology Member: MHMRC Women in Science Working Committee Member: The Australian Society for Editorial Board Member: Chromosoma; Psychiatric Research Epigenetics; Epigenetics & Chromatin; Member: Australasian Society for Open Biology Immunology Member: Collegium Internationale Neuro- Psychopharmacologicum (CINP) Member: Lorne Genome society Member: American Association of Immunologists Member: Australian Society for Biochemistry & Molecular Biology Member: JCSMR Executive Committee Dr Kerong Zhang Member: American Society for Member: The Australasian Genomics Microbiology Professor Bruce Walmsley Technologies Association (AMATA) Session Chair: ComBio Conference, Editorial Board Member: Biology Image Adelaide, SA Library Yicheng Zhu Presenter: Hearing Awareness Week ACT Member: The Australian Society for Dr Krisztina Valter Expo, Canberra, ACT Medical Research (ASMR) Co-Chair: Block1 ANU Medical School, Member: Australasian Neuroscience Member: Genetics Society of AustralAsia ANU, Canberra, ACT Society (GSA) Member: Year 1 and Year 2 Examination Member: Society for Neuroscience USA Committees, ANU Medical School, ANU, Canberra, ACT Professor Ma-Li Wong Member: Phase 1 Committee, ANU Associate Editor: Molecular Psychiatry Medical School, ANU, Canberra, ACT Editorial Board Member: The Member: Medical Science Committee, Pharmacogenomics Journal; Clinical ANU Medical School, ANU, Canberra, Pharmacology, Advances and ACT Applications; Journal of Experimental Member: Neuroscience Honours Pharmacology. Committee, JCSMR, ANU, Canberra, Reviewer: Melbourne Health, Royal ACT Melbourne Hospital, Melbourne, VIC Member: Association for Research in Fellow: The Royal Australian and New Vision and Ophthalmology (ARVO) Zealand College of Psychiatrists Member: European Vision and Eye Member: PhD Scholarship Committee, Research (EVER) JCSMR
Annual Review 2013 129 130 The John Curtin School of Medical Research Support to the School
Grants Financial Overview Donors Corporate Sponsorship
Annual Review 2013 131 GRANTS
Agency for Science Australian Research Council Professor T Preiss and Dr T Technology and Research Centre of Excellence in Beilharz (A* Star) – Singapore Vision Science Role of mRNA polyadenylation control in gene expression Dr A Tan Professor T Maddess and Dr A $35,000 Post-Doctoral Support James Professor CG Vinuesa $20,839 Advanced multifocal analysis of blinding diseases The role of Roquin in microRNA function and decay $273,000 American Australian $170,000 Association Dr J Paris and Dr K Valter-Kocsi Structure and function of primate macula Dr R Sweet Australian Research Council $168,000 US to Australia Fellowship LIEF Grant $30,000 Australian Research Council Professor L Botten, Professor D Leinweber, Professor L Radom, Discovery Project Grants Australian Centre for HIV Professor J Gready et al. and Hepatitis Virology Dr NA Beard, Professor AF Strengthening merit-based access and Research Grant Dulhunty and Professor R Dirksen support at the new National Computing Infrastructure petascale supercomputing How triadin and junctin communicate facility Dr C Ranasinghe and Dr R with ryanodine receptors deep within Jackson a calcium store to determine skeletal $650,000 Design an effective poxvirus based muscle contraction vaccine for HIV-1 by modulating immune $100,000 Bayer Bioscience Research cell milieu using an IL-10 antagonist Co-operative Grant $136,000 Dr VR Daria, Associate Professor C Stricker, Professor S Redman, Professor J Gready ANU Discovery Translation Professor H Bachor and Professor Design of variant Ribulose-1 G Stuart Fund 5-bisphosphate caboxylase/oxygenase Using light to probe brain activity in 3D proteins with improved catalytic efficiency and substrate specificity Professor M-L Wong and $143,000 Professor J Licinio $415,092 Professor S Easteal, Dr R Williams, Evaluating a novel pharmacological Dr LS Jermiin and Dr DG MacArthur treatment for obesity and metabolic Beta Therapeutics Pty Ltd syndrome The role of short tandem repeat DNA Discovery Translation Fund variation in the evolution of human $81,836 psychological diversity Project Grant $82,000 ANU Major Equipment Professor C Parish and Dr C Committee Associate Professor G Huttley and Simeonovic Dr VB Yap $114,669 Professor T Preiss Improving the accuracy of phylogenetic Understanding transcriptome dynamics: reconstruction by improving models of Bioplatforms Australia Ltd/ quantitating changes in cellular RNA sequence divergence Education Investment Fund $100,000 $80,000 Professor S Easteal Professor T Lamb, Professor I Professor D Tremethick $200,000 A high-capacity high-throughput Potter, Associate Professor N Hart, superspeed centrifuge Professor S Collin and Professor D $49,000 Hunt The evolution of light detection and its impact on early vertebrate evolution $10,000
132 The John Curtin School of Medical Research Cancer Australia Garnett Passe and National Health & Medical Dr A Blackburn and Professor P Rodney Williams Memorial Research Council Australia Board Foundation Fellowship Targeting of two aspects of metabolism (TOTAM) for cancer therapy Professor B Walmsley Professor CC Goodnow $20,000 How does sound-induced quantal New strategies to reveal the genetic and neurochemical release in the cochlea cellular basis of immunological disease generate impulses in the auditory nerve? Commonwealth Department $800,000 $96,246 of Regional Australia Local National Health & Medical Government Arts and Sport Grains Research and Research Council Career Anti-Doping Research Development Corporation Development Fellowships Program Professor J Gready Dr N Beard Professor S Easteal, Dr J Identifying Wheat Germplasm with Control of cardiac and skeletal Henderson, Dr R Williams, Ms S Superior Rubiscos for Breeding for contractility by luminal calcium store load Tan, Dr C Gore and Dr M Ashenden Increased Drought Tolerance in health and disease Novel high throughput c-DNA sequencing $349,990 $96,040 to identify a genomic signature for autologous blood transfusion Human Frontier Science Dr A Enders $17,016 Program Investigating B cell development maintenance and high-affinity antibody Department of Industry, Dr R Sweet production by ENU mutagenesis Innovation, Climate The role of T cell derived interferon $97,769 Change Science Research gamma in lupus associated anxiety and depression National Health & Medical and Tertiary Education $36,150 Research Council (DIICCSRTE) Australia-India Development Grant Strategic Research Fund Juvenile Diabetes Research Dr C Ranasinghe and Professor I Dr D Rangasamy, Professor P Foundation – National Ramshaw Board and Dr N Lenka Health and Medical A novel strategy to enhance T cell- An insertational mutagenesis approach Research Council Special mediated immunity to vaccine antigens to identify epigenetic factors that control Program Grant embryonic stem cell differentiation $76,834 $90,000 Professor C Parish, Dr C Simeonovic, Dr C Freeman and Dr National Health & Medical Department of Industry, G Hoyne Research Council Early Innovation, Climate Role of heparan sulfate, heparanase and Career Fellowship heparanase inhibitors in the development Change Science Research and prevention of Type I diabetes Dr D Ryan and Tertiary Education $600,000 Investigations into the mechanism and (DIICCSRTE) National function of the chromatin remodelling complex SWI-SNF Collaborative Research Medical Advances Without Infrastructure Strategy Animals Trust $68,723
Dr S Winslade, Professor C Associate Professor B Lidbury Goodnow and Ms A McKenzie Sponsorship ANU Hosted Fellowship Australian Phenomics Network Super Program Science Project $151,885 $4,000,000
Annual Review 2013 133 National Health & Medical National Health & Medical Professor AF Dulhunty, Dr NA Research Council Research Council Program Beard and Dr DR Laver Equipment Grant Grant Regulation of calcium release channels (RyR2) in healthy and failing hearts Dr A Cook Professor C MacKay, Professor J $108,525 Countess cell counter Sprent, Professor CC Goodnow, Professor AF Dulhunty, Dr MG Dr F MacKay, Professor CG $7,199 Casarotto and Professor PG Vinuesa, Professor A Basten, Board Professor A Dulhunty Professor B Fazekas de St Groth, Dr Prep Cell Pump and Fraction Collector S Tangye and Dr R Brink DHPR β subunit binding to a variably spliced region of RyR1: A role in EC $20,725 Molecular and cellular studies of the coupling and myotonic dystrophy adaptive immune response in health and Professor S Easteal disease $178,902 MultiNA MCE-202 microchip $1,086,841 Dr A Enders and Professor C electrophoresis system for DNA/RNA analysis Goodnow National Health & Medical Understanding the pathogenesis and $6,515 Research Council Project heterogeneity of autoimmunity as failure of multiple steps Professor C Goodnow Grants ELISA Plate Reader $162,209 Dr NA Beard, Professor AF $42,780 Associate Professor G Huttley and Dulhunty and Professor S Gyorke Dr W Meyer Dr D Ryan Unique isoform-specific regulation of Uncovering the genetic basis of fungal Infors MultiTron2 Temperature Controlled cardiac ryanodine receptors by calcium virulence Shaking Incubator store proteins $82,893 $25,130 $135,375
Professor D Tremethick Associate Professor JM Bekkers Associate Professor G Karupiah and Dr G Chaudhri New “HyD” high sensitivity detector for and Dr M Larkum Understanding the key attributes of CD8 Leica confocal microscope Neural circuits for odour-processing in the T cell receptor transfer as an antiviral $27,681 rodent piriform cortex in vitro strategy and harnessing the process to $173,902 combat persistent viral infections National Health & Medical Associate Professor JM Bekkers $197,244 Research Council and Professor S Nelson Associate Professor G Karupiah Postdoctoral Training Excitability and hyperexcitability of neural and Dr T Newsome circuits in the rodent piriform cortex Fellowships Actin-based motility as a virulence $113,250 mechanism and potential as an antiviral Dr R Haddock target Obesity and diabetes: Understanding Professor PG Board, Professor AF cardiovascular risk factors and Dulhunty, Dr MG Casarotto and $75,000 identification of novel targets for treatment Professor L Arnolda Professor T Maddess, Dr A $71,812 New cardiac ryanodine receptor inhibitors James, Dr C Carle, Professor C for the treatment of heart failure Dr I Parish Nolan and Dr R Essex $197,244 Regulation of CD8+ T cell dysfunction Novel functional testing for early diabetic retinopathy during tolerance and chronic viral infection Professor AF Dulhunty, Professor L $71,250 Arnolda, Dr A Blackburn and Dr N $232,761 Beard Professor C Parish and Dr B Quah Anthracyclines disrupt Ca2+ signalling in Analysis of antigen receptor sharing by T cardiomyocytes: a contribution to cardiac and B lymphocytes toxicity $173,902 $143,775
134 The John Curtin School of Medical Research Professor T Preiss and Dr J Professor CG Vinuesa, Dr D Yu and Professor C Goodnow, Associate Clancy Associate Professor A Liston Professor G Karupiah, Dr R Exploring the role of miRNA and Specialised subsets of T follicular helper Bertram and Professor R Ulevitch target processing variability in cardiac cells in the control of infection and Control systems governing short vs long hypertrophy immune pathology term humoral and cellular immunity $194,895 $166,130 $802,954 (in conjunction with The Scripps Research Institute, USA) Professor T Preiss and Dr J Professor I Young and Professor P Mackay Foster Professor C Goodnow, Professor R Designer RNA-binding proteins for Targeting miRNA to inhibit leukocyte Ulevitch and Professor B Beutler research and therapeutic purposes differentiation: a novel anti-inflammatory Systems approach to immunity and $25,000 approach for the treatment of asthma inflammation $96,835 Professor T Preiss and Dr N $630,442 (in conjunction with The Scripps Research Institute, USA) McMillan Professor I Young, Professor D Ollis RNA interference and the immune system and Dr J Murphy Professor C Goodnow, Professor R $36,750 Cytokine-driven allergic inflammation: Ulevitch and Professor B Beutler characterization of two isoform-specific Systems approach to immunity and Dr C Ranasinghe modes of IL-3 receptor activation and inflammation - Annotation investigation of new receptor-associated Enhancement of mucosal immunity and $128,418 (in conjunction with The signalling partners CTL avidity against HIV-1 Scripps Research Institute, USA) $82,951 $199,895
Dr K Randall Clive and Vera Ramaciotti National Health & Medical Foundation Major Research Investigation of cellular abnormalities Research Council Research and synapse formation in DOCK8 Award immunodeficiency Fellowships $102,500 Professor CC Goodnow and Dr A Professor T Preiss Enders Professor GJ Stuart $128,371 Ramaciotti Immunization Genomics Properties of dendritic spines and their Professor C Vinuesa Laboratory role in synaptic plasticity $128,371 $200,000 $108,250
Professor GJ Stuart National Health & Medical Cortical mechanisms underlying binocular Research Council Senior vision Research Fellowship $119,736 Associate Professor G Karupiah Professor GJ Stuart $128, 371 Regulation of cortical excitability by GABAB receptors National Institutes of Health $109,736 (USA) Project Grants Professor DJ Tremethick Professor AF Dulhunty and How chromatin compaction is regulated and its link with disease Professor R Dirksen $158,902 Control of calcium movements in muscle $19,278 (in conjunction with The Professor DJ Tremethick University of Rochester, NY, USA) Mechanisms underpinning the epigenetic code and the role of histone variants $181,130
Annual Review 2013 135 FINANCIAL OVERVIEW
Staff numbers 2012 2013 Academic staff * 77.66 75.1 General staff — administration, technical & support *º 139.73 140.45 HDR students (EFTSL) 80.5 81.0 Postgraduate coursework students (EFTSL) 1.0 11.1 Undergraduate Students 23.9
* The figures are for Full Time Equivalents, as at 31 December, and exclude casuals º Includes Animal Service Facility staff Recurrent and Special Purpose Funds $ Total Income 2012 2013 Student Fees 256,498 464,760 Other Income 22,539,733 23,595,143 Internal Sales 4,824,596 3,986,467 Transfer from other 13,609,314 6,419,273 Internal Allocations 374,239 2,495,286 Operating Grant 17,754,231 17,930,737 Investment Income 167,841 59,501 Total Income 59,190,771 54,951,167
Total Expenditure Salaries & Related Costs 25,904,582 24,999,564 Equipment - Capital 1,585,596 4,309,383 Equipment - Non-Capital 633,287 533,048 Scholars Expenses 931,822 902,531 Utilities & Maintenance 2,080,295 2,766,480 Travel Field & Survey Expenses 900,232 789,586 Expendable Research Materials 10,434,045 8,788,626 Other Expenses 8,526,002 8,238,979 Transfer to other 9,100,251 7,661,040 Total Expenditure 60,096,112 58,989,238
Net Current Year Operating Result 905,342 4,038, 070 Prior Year Cash Result 19,674,634 21,225,223 Net Operating Position 18,769,292 17,187,153
136 The John Curtin School of Medical Research DONORS
The Director, staff and students of Mrs Mary Appleby Mrs Thelma Maurice JCSMR are extremely grateful for the Mr Jock Bartram Mr John McConville continued generosity and support of our friends and donors. Ms Meryl Bartram Ms Joan McDonald Gifts and bequests to the School are used Ms Pam Bartram Ms Jennifer McEniery to fund vital medical research projects Mr Gary Borradale Mrs Lyn McKnight as well as providing scholarships and Mr Ron Brown Ms Jenni McMullan purchasing specialised equipment. Your support can be provided in a number Mrs Joan Byrne Mr John Milne of ways including a gift or bequest that Ms J Cawthray Mr Phil Muttukumaru may assist in funding a particular area of research, or a scholarship or prize. Mr Norman Clough J Newton If you would like to discuss options for Ms Jennifer Copley Mr John O’Dor supporting JCSMR, please contact: Ms Lyn Dalton Mrs L O’Sullivan Dr Madeleine Nicol Dr Maria Dano Ms Rosslyn Paul T 02 6125 2577 Mrs Daphne Douglas Ms Glenis Pearson E [email protected] Mr Keith Dunstan Mr Philip Pickersgill The Tom Efkarpidis Foundation Mr Neil Power Our thanks go to those who have so Mrs Judith Ford Research Australia generously supported The John Curtin Medical Research Foundation at ANU Mr Jock Gavel Mr Denis Regan during 2013. Mr Albert Green 3 RNSWR Association Details can be found on their website: Mr Peter Griffith Mrs Rhonda Robinson jcmrf.anu.edu.au Mrs Bev Harvey Mr Gordon Rogers Mr Bradley Harvey The Sarah-Grace Sarcoma Foundation Ms Cynthia Harvey Mrs Gwendolyn Schulz Mr Kieren Harvey Mr Felix Scudamore Mr Robin Harvey Ms Lea Sublett Mr Leslie Hawkes Ms Sue Sullivan Mr Ross Holmes Mr John Tuck The John James Foundation Mr Robert Tupper Staff of Joint Operations Command, Mr Thomas Tyrrell Department of Defence Ms Debbie Vipond Mrs Robyn Jones Mr Gary Vipond Mr David Kennemore Mr Brett Walker Mr Ellis Lalor Mr Steven Warren Mr Gordon Lauder Mr Jeff West Mr John Lee Mrs Susan West Ms Louise Lee Mr Robin Yule Miss Lydia Lobb Professor Teddy Maddess Ms Dorothy Mangos
Annual Review 2013 137 CORPORATE SPONSORS
138 The John Curtin School of Medical Research
The John Curtin School of Medical Research ANU College of Medicine, Biology and Environment 131 Garran Road Acton ACT 2601 Australia T +61 2 6125 2550 F +61 2 6125 2337 E [email protected] W jcsmr.anu.edu.au CRICOS #00120C MO_140180