G3: Genes|Genomes|Genetics Early Online, published on February 7, 2017 as doi:10.1534/g3.116.036327 1 Triploid Production from Interspecific Crosses of Two Diploid Perennial Helianthus 2 with Diploid Cultivated Sunflower (Helianthus annuus L.) 3 4 Zhao Liu1*, Gerald J. Seiler2, Thomas J. Gulya2, Jiuhuan Feng3, Khalid Y. Rashid4, Xiwen Cai1, 5 Chao-Chien Jan2* 6 7 1Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USA 8 2USDA-ARS, Northern Crop Science Laboratory, Fargo, ND 58102, USA 9 3Plant Science Department, South Dakota State University, Brookings, SD 57007, USA 10 4Agriculture and Agri-Food Canada, Morden, Manitoba, R6M 1Y5, Canada 11 12 Key words: Helianthus; triploids; genomic in situ hybridization (GISH); mitotic analysis; 13 meiotic analysis 14 15 Corresponding author: Chao-Chien Jan 16 Mailing address: USDA-ARS, NCSL, 1605 Albrecht Blvd N, Fargo, ND58102-2765, USA 17 Phone: 701-239-1319 18 E-mail:
[email protected] 19 20 Co-corresponding author: Zhao Liu 21 Mailing address: USDA-ARS, NCSL, 1605 Albrecht Blvd N, Fargo, ND58102-2765, USA 22 Phone: 701-239-1324 23 E-mail:
[email protected] 24 1 © The Author(s) 2013. Published by the Genetics Society of America. 25 Running title: Novel triploid production in Helianthus 2 26 ABSTRACT 27 Wild Helianthus species are a valuable genetic resource for the improvement of cultivated 28 sunflower. We report the discovery and characterization of a unique high frequency production 29 of triploids when cultivated sunflower was pollinated by specific accessions of diploid 30 Helianthus nuttallii T. &. G. and H.