Ecosystem Services Provided by the Little Things That Run the World
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Microsatellite Loci Isolated from the Mediterranean Species Cicada Barbara (Stål) and C
Molecular Ecology Notes (2002) 2, 173–175 PRIMERBlackwell Science, Ltd NOTE Microsatellite loci isolated from the Mediterranean species Cicada barbara (Stål) and C. orni L. (Hemiptera, Cicadoidea) S. G. SEABRA,*† H. R. WILCOCK,* J. A. QUARTAU† and M. W. BRUFORD* *School of Biosciences, Cardiff University, Main Building, Park Place, Cardiff, CF10 3TL, UK, †Centro de Biologia Ambiental e Departamento de Zoologia e Antropologia, Bloco C2, 3° Piso, Faculdade de Ciências de Lisboa, Campo Grande 1700 Lisboa, Portugal Abstract We describe the isolation of six polymorphic microsatellites for Cicada barbara (Stål), four of which are also polymorphic for the closely related Cicada orni L. Cicadas from several sites in the Iberian Peninsula, North Africa (C. barbara) and Greece (C. orni) were genotyped at these loci. Polymorphism is higher than that previously obtained with allozymes for these species. One locus allows species diagnosis (nonoverlapping allele size ranges) between C. barbara and C. orni and the others have some exclusive alleles for each species. Keywords: cicadas, Cicada barbara, Cicada orni, insects, microsatellites Received 6 November 2001; revision received 21 December 2001; accepted 21 December 2001 The genus Cicada L. (Hemiptera, Cicadoidea) includes agarose gel by electroelution into dialysis tubing and some sibling species that are morphologically very similar purified using Centricon Microconcentrators (Amicon). but distinguishable by the specific calling songs produced The fragments were enriched for CA and GA repeats using by males for mate recognition. Cicada barbara and C. orni are biotin-labelled probes and then ligated into a pUC18 vector two of these species that exist in sympatry in some areas (Amersham Pharmacia Biotech) (Hammond et al. -
Are Biologicals Smart Mole Cricket Control?
Are biologicals smart mole cricket control? by HOWARD FRANK / University of Florida ost turf managers try to control mole faster than the biopesticides, but the biopesticides cricket pests with a bait, or granules or affect a narrower range of non-target organisms and liquid containing something that kills are more environmentally acceptable. The "biora- them. That "something" may be chemi- tional" chemicals are somewhere in between, be- M cause they tend to work more slowly than the tradi- cal materials (a chemical pesticide) or living biologi- cal materials (a biopesticide). tional chemicals, and to have less effect on animals Some of the newer chemical materials, called other than insects. "biorationals," are synthetic chemicals that, for ex- Natives not pests ample, mimic the action of insects' growth hor- The 10 mole cricket species in the U.S. and its mones to interfere with development. territories (including Puerto Rico and the Virgin Is- The biological materials may be insect-killing ne- lands) differ in appearance, distribution, behavior matodes (now available commercially) or fungal or and pest status. bacterial pathogens (being tested experimentally). In fact, the native mole crickets are not pests. These products can be placed exactly where they Our pest mole crickets are immigrant species. are needed. In general, the chemical pesticides work The three species that arouse the ire of turf man- agers in the southeastern states all belong Natural enemies to the genus Scapteriscus. They came from Introducing the specialist natural ene- South America, arriving at the turn of the mies from South America to the southeast- century in ships' ballast. -
Native and Invasive Ants Affect Floral Visits of Pollinating Honey Bees in Pumpkin Flowers (Cucurbita Maxima)
www.nature.com/scientificreports OPEN Native and invasive ants afect foral visits of pollinating honey bees in pumpkin fowers (Cucurbita maxima) Anjana Pisharody Unni1,3, Sajad Hussain Mir1,2, T. P. Rajesh1, U. Prashanth Ballullaya1, Thomas Jose1 & Palatty Allesh Sinu1* Global pollinator decline is a major concern. Several factors—climate change, land-use change, the reduction of fowers, pesticide use, and invasive species—have been suggested as the reasons. Despite being a potential reason, the efect of ants on fowers received less attention. The consequences of ants being attracted to nectar sources in plants vary depending upon factors like the nectar source’s position, ants’ identity, and other mutualists interacting with the plants. We studied the interaction between fower-visiting ants and pollinators in Cucurbita maxima and compared the competition exerted by native and invasive ants on its pollinators to examine the hypothesis that the invasive ants exacerbate more interference competition to pollinators than the native ants. We assessed the pollinator’s choice, visitation rate, and time spent/visit on the fowers. Regardless of species and nativity, ants negatively infuenced all the pollinator visitation traits, such as visitation rate and duration spent on fowers. The invasive ants exerted a higher interference competition on the pollinators than the native ants did. Despite performing pollination in fowers with generalist pollination syndrome, ants can threaten plant-pollinator mutualism in specialist plants like monoecious plants. A better understanding of factors infuencing pollination will help in implementing better management practices. Humans brought together what continents isolated. One very conspicuous evidence of this claim rests in the rearranged biota. -
Lesson: Key Details Lesson Topic: Identify and Explain Key Details Question 1
Lesson: Key Details Lesson Topic: Identify and explain key details Question 1: Read the text below. Sea Stars Written by Lindsey Crowe There are many different kinds of sea stars. For example, some sea stars have five legs and some have ten legs. Some have skinny arms and some have wide arms. Sea stars are found in the ocean. Some sea stars live in warm oceans. Some live in cold oceans. They can be found in deep and shallow waters. Some people call sea stars star fish. Sea stars are not fish. They do not have gills, fins, or bones. Which of the following key details supports the idea that there are many different kinds of sea stars? There are many different kinds of sea stars. Sea stars are found in the ocean. Some sea stars have five legs and some have ten legs. They do not have gills, fins, or bones. Question 2: Read the text below. There are many different kinds of sea stars. For example, some sea stars have five legs and some have ten legs. Some have skinny arms and some have wide arms. Why is the underlined sentence an important key detail? because it explains why the author took pictures and wrote a text about sea stars because it is an important fact that we need to know to understand about different kinds of sea stars because it explains what kind of sea star is in the photograph because it gives the names of different types of sea stars Question 3: Read the text below. -
Federal Register / Vol. 61, No. 42 / Friday, March 1, 1996 / Proposed
8014 Federal Register / Vol. 61, No. 42 / Friday, March 1, 1996 / Proposed Rules under CERCLA are appropriate at this FOR FURTHER INFORMATION CONTACT: is currently known only from Santa time. Consequently, U.S EPA proposed Leslie K. Shapiro, Mass Media Bureau, Cruz County, California. The five known to delete the site from the NPL. (202) 418±2180. populations may be threatened by the EPA, with concurrence from the State SUPPLEMENTARY INFORMATION: This is a following factors: habitat fragmentation of Minnesota, has determined that all synopsis of the Commission's Notice of and destruction due to urban appropriate Fund-financed responses Proposed Rule Making, MM Docket No. development, habitat degradation due to under CERCLA at the Kummer Sanitary 96±19, adopted February 6, 1996, and invasion of non-native vegetation, and Landfill Superfund Site have been released February 20, 1996. The full text vulnerability to stochastic local completed, and no further CERCLA of this Commission decision is available extirpations. However, the Service finds response is appropriate in order to for inspection and copying during that the information presented in the provide protection of human health and normal business hours in the FCC petition, in addition to information in the environment. Therefore, EPA Reference Center (Room 239), 1919 M the Service's files, does not provide proposes to delete the site from the NPL. Street, NW., Washington, DC. The conclusive data on biological vulnerability and threats to the species Dated: February 20, 1996. complete text of this decision may also be purchased from the Commission's and/or its habitat. Available information Valdas V. -
Recovery Plan for Northeastern Beach Tiger Beetle
Northeastern Beach Tiger Beetle, (Cincindela dorsalisdorsal/s Say) t1rtmow RECOVERY PLAN 4.- U.S. Fish and Wildlife Service SFAVI ? Hadley, Massachusetts September 1994 C'AZ7 r4S \01\ Cover illustration by Katherine Brown-Wing copyright 1993 NORTHEASTERN BEACH TIGER BEETLE (Cicindela dorsalis dorsalis Say) RECOVERY PLAN Prepared by: James M. Hill and C. Barry Knisley Department of Biology Randolph-Macon College Ashland, Virginia in cooperation with the Chesapeake Bay Field Office U.S. Fish and Wildlife Service and members of the Tiger Beetle Recovery Planning-Group Approved: . ILL Regi Director, Region Five U.S. Fish and Wildlife Service Date: 9 29- ~' TIGER BEETLE RECOVERY PLANNING GROUP James Hill Philip Nothnagle Route 1 Box 2746A RFD 1, Box 459 Reedville, VA Windsor, VT 05089 Judy Jacobs Steve Roble U.S. Fish and Wildlife Service VA Natural Heritage Program Annapolis Field Office Main Street Station 177 Admiral Cochrane Drive 1500 East Main Street Annapolis, MD 21401 Richmond, VA 23219 C. Barry Knisley Tim Simmons Biology Department The Nature Conservancy Massachusetts Randolph-Macon College Field Office Ashland, VA 23005 79 Milk Street Suite 300 Boston, MA 02109 Laurie MacIvor The Nature Conservancy Washington Monument State Park 6620 Monument Road Middletown, MD 21769 EXECUTIVE SUMMARY NORTHEASTERN BEACH TIGER BEETLE RECOVERY PLAN Current Status: This tiger beetle occurred historically "in great swarms" on beaches along the Atlantic Coast, from Cape Cod to central New Jersey, and along Chesapeake Bay beaches in Maryland and Virginia. Currently, only two small populations remain on the Atlantic Coast. The subspecies occurs at over 50 sites within the Chesapeake Bay region. -
Vol. 33, Number 3
NO.3 May/June 1991 eDITOR o( the LEPIDOPTERISTS' SOCIETY June Preston 832 Sunset Dr Lawrence. KS 66044 U.S.A. • •• • •• • • . -. •• .-.-. ...- . • • •• ASSOCIATE eDITOR ZONE COORDINATORS 1. Ken Philip 6. Ed Knudson 10. Dave Winter 2. Jon Shepard 7. Ross Layberry 11. J.e.E. Riotte Ripples 3. Bob Langston 8. Les Ferge 12. Eduardo Welling M. Jo Brewer 4. Ray Stanford 9. Andy Beck 13. Boyce Drummond 5. Ron A. Royer • •• I • ....__... ••_ ...._._....__~._••_ ... ••_._..._._....__....__~.__•••__...__...__• BUTIERFLY DIVERSITY AND CONSERVATION IN THE RONDONIAN RAIN FOREST OF BRAZIL: A PROGRESS REPORT ON THE NEW RESERVE The state of Rondonia in west central Brazil apparently 27 lepidopterists in November 1990 and another 26 has the highest butterfly diversity per square kilometer in the lepidopterists in December 1990 has added more than 200 world, with an estimated 1,500 to 1,600 species residing additional species in these two months to the identified list, within several square kilometers in the central part of that raising the total to over 1,060 identified species inhabiting state near Ariquemes. After a major March 1989 expedition the area around the Fazenda Rancho Grande field station. We to the Rondonian rain forest around the Fazenda Rancho Grande still project that the "final" list will amount to around 1,600 involving more than two dozen members of the Lepidopterists' butterfly species! Society, it was clear that this incredible diversity - first As mentioned in Emmel (1989) and Emmel and Austin noted two years earlier in March 1987 - was about to (1990), the sampling efforts have centered on the Fazenda disappear under the hand of man, through cutting and burning Rancho Grande, a 750-hectare tract (1,875 acres) owned by of the tropical rain forest to create temporary crop land and the Harald Schmitz family. -
Infectivity of Housefly, Musca Domestica
b r a z i l i a n j o u r n a l o f m i c r o b i o l o g y 4 7 (2 0 1 6) 807–816 ht tp://www.bjmicrobiol.com.br/ Environmental Microbiology Infectivity of housefly, Musca domestica (Diptera: Muscidae) to different entomopathogenic fungi ∗ Muzammil Farooq, Shoaib Freed Bahauddin Zakariya University, Faculty of Agricultural Sciences and Technology, Laboratory of Insect Microbiology and Biotechnology, Multan, Punjab, Pakistan a r t i c l e i n f o a b s t r a c t Article history: The housefly Musca domestica is a worldwide insect pest that acts as a vector for many Received 26 February 2014 pathogenic diseases in both people and animals. The present study was conducted to eval- Accepted 4 March 2016 uate the virulence of different local isolates of Beauveria bassiana, Metarhizium anisopliae and Available online 4 July 2016 Isaria fumosorosea on M. domestica using two bioassay techniques: (1) adult immersion and Associate Editor: Carlos Pelleschi (2) a bait method applied to both larvae and adults. The results showed evidence of a broad Taborda range of responses by both stages (larvae and adults) to the tested isolates of B. bassiana, M. anisopliae and I. fumosorosea. These responses were concentration-dependent, with mor- Keywords: tality percentages ranging from 53.00% to 96.00%. Because it resulted in lower LC50 values and a shorter lethal time, B. bassiana (Bb-01) proved to be the most virulent isolate against Entomopathogenic fungi Fecundity both housefly larvae and adults. -
Dragonflies and Damselflies in Your Garden
Natural England works for people, places and nature to conserve and enhance biodiversity, landscapes and wildlife in rural, urban, coastal and marine areas. Dragonflies and www.naturalengland.org.uk © Natural England 2007 damselflies in your garden ISBN 978-1-84754-015-7 Catalogue code NE21 Written by Caroline Daguet Designed by RR Donnelley Front cover photograph: A male southern hawker dragonfly. This species is the one most commonly seen in gardens. Steve Cham. www.naturalengland.org.uk Dragonflies and damselflies in your garden Dragonflies and damselflies are Modern dragonflies are tiny by amazing insects. They have a long comparison, but are still large and history and modern species are almost spectacular enough to capture the identical to ancestors that flew over attention of anyone walking along a prehistoric forests some 300 million river bank or enjoying a sunny years ago. Some of these ancient afternoon by the garden pond. dragonflies were giants, with This booklet will tell you about the wingspans of up to 70 cm. biology and life-cycles of dragonflies and damselflies, help you to identify some common species, and tell you how you can encourage these insects to visit your garden. Male common blue damselfly. Most damselflies hold their wings against their bodies when at rest. BDS Dragonflies and damselflies belong to Dragonflies the insect order known as Odonata, Dragonflies are usually larger than meaning ‘toothed jaws’. They are often damselflies. They are stronger fliers and referred to collectively as ‘dragonflies’, can often be found well away from but dragonflies and damselflies are two water. When at rest, they hold their distinct groups. -
Rapid and Simple Species Identification of Cicada Exuviae
insects Article Rapid and Simple Species Identification of Cicada Exuviae Using COI-Based SCAR Assay Pureum Noh, Wook Jin Kim, Jun-Ho Song , Inkyu Park , Goya Choi and Byeong Cheol Moon * Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea; [email protected] (P.N.); [email protected] (W.J.K.); [email protected] (J.-H.S.); [email protected] (I.P.); [email protected] (G.C.) * Correspondence: [email protected]; Tel.: +82-61-338-7100 Received: 5 February 2020; Accepted: 4 March 2020; Published: 6 March 2020 Abstract: Cicadidae periostracum (CP), the medicinal name of cicada exuviae, is well-known insect-derived traditional medicine with various pharmacological effects, e.g., anticonvulsive, anti-inflammatory, antitussive, and anticancer effects; it is also beneficial for the treatment of Parkinson’s disease. For appropriate CP application, accurate species identification is essential. The Korean pharmacopoeia and the pharmacopoeia of the People’s Republic of China define Cryptotympana atrata as the only authentic source of CP. Species identification of commercially distributed CP based on morphological features, however, is difficult because of the combined packaging of many cicada exuviae in markets, damage during distribution, and processing into powder form. DNA-based molecular markers are an excellent alternative to morphological detection. In this study, the mitochondrial cytochrome c oxidase subunit I sequences of C. atrata, Meimuna opalifera, Platypleura kaempferi, and Hyalessa maculaticollis were analyzed. On the basis of sequence alignments, we developed sequence-characterized amplified-region (SCAR) markers for efficient species identification. These markers successfully discriminated C. -
Mole Crickets Scapteriscus Spp
Mole Crickets Scapteriscus spp. Southern mole cricket, Scapteriscus borellii Tawny mole cricket, Scapteriscus vicinus DESCRIPTION OF INSECT All stages live in the soil and are rarely see on the surface. Immature stage Nymphs of both species are similar in appearance to adults, but lack wings. Nymphs proceed through 8-10 instars ranging in size from 0.2 to 1.25 inches in length. Each instar is progressively larger with wing buds apparent on later instars. Color varies from gray to brown. Pronotum (large shield behind head) with distinctive mottling or spots, depending on species and location. Mature stage Adults are somewhat cylindrically shaped, light colored crickets 1.26 to 1.38 inches in length. Adults have two pairs of wings, but only fly at night during two brief flight periods in fall and early spring. Spring flights are generally more extensive than fall flights. Damaging stage(s) Both nymphs and adults cause damage Predictive models (degree day, plant phenology, threat temperatures, other) Eggs being to hatch at threat temperatures of 65° F and higher (spring/early summer in most locations). Egg-laying and hatch timing are affected by soil moisture. Threat temperatures can be used to trigger preventive treatments. See the article, “Threat temperatures” for more information. Preventive treatments should be applied prior to egg-hatch (early June) or at the time of peak hatch (last week of June, first week of July in most years and locations). Weekly soap flushes in June and early July is the best method to determine when hatch is occurring, and the best time to treat. -
Coccidology. the Study of Scale Insects (Hemiptera: Sternorrhyncha: Coccoidea)
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Ciencia y Tecnología Agropecuaria (E-Journal) Revista Corpoica – Ciencia y Tecnología Agropecuaria (2008) 9(2), 55-61 RevIEW ARTICLE Coccidology. The study of scale insects (Hemiptera: Takumasa Kondo1, Penny J. Gullan2, Douglas J. Williams3 Sternorrhyncha: Coccoidea) Coccidología. El estudio de insectos ABSTRACT escama (Hemiptera: Sternorrhyncha: A brief introduction to the science of coccidology, and a synopsis of the history, Coccoidea) advances and challenges in this field of study are discussed. The changes in coccidology since the publication of the Systema Naturae by Carolus Linnaeus 250 years ago are RESUMEN Se presenta una breve introducción a la briefly reviewed. The economic importance, the phylogenetic relationships and the ciencia de la coccidología y se discute una application of DNA barcoding to scale insect identification are also considered in the sinopsis de la historia, avances y desafíos de discussion section. este campo de estudio. Se hace una breve revisión de los cambios de la coccidología Keywords: Scale, insects, coccidae, DNA, history. desde la publicación de Systema Naturae por Carolus Linnaeus hace 250 años. También se discuten la importancia económica, las INTRODUCTION Sternorrhyncha (Gullan & Martin, 2003). relaciones filogenéticas y la aplicación de These insects are usually less than 5 mm códigos de barras del ADN en la identificación occidology is the branch of in length. Their taxonomy is based mainly de insectos escama. C entomology that deals with the study of on the microscopic cuticular features of hemipterous insects of the superfamily Palabras clave: insectos, escama, coccidae, the adult female.