Tasmannia Lanceolata
ASPECTS OF LEAF AND EXTRACT PRODUCTION from Tasmannia lanceolata by Chris Read, B. Agr.Sc. Tas. Submitted in fulfillment of the requirements for the Degree of Doctor of Philosophy University of Tasmania, Hobart December 1995 ' s~, ... ~~ \ ·'(11 a_C\14 \t\J. \I ' This thesis contains no material which has been accepted for the award of any other degree or diploma in any University, and to the best of my knowledge, contains no copy or paraphrase of material previously written or published by any other person except where due reference is given in the text. University of Tasmania HOBART March 1996 This thesis may be made available for loan and limited copying in accordance with the Copyright Act 1968 University of Tasmania HOBART March 1996 Abstract This thesis examines several aspects of the preparation, extraction and analysis of solvent soluble compounds from leaf material of Tasmannia lanceolata and reports a preliminary survey of extracts of some members of the natural population of the species in Tasmania. A major constituent of these extracts, polygodial, was shown to be stored within specialised idioblastic structures scattered throughout the mesophyll, and characterised by distinctive size and shape, and a thickened wall. The contents of these cells were sampled directly, analysed and compared with the composition of extracts derived from ground, dry whole leaf. This result was supported by spectroscopic analysis of undisturbed oil cells in whole leaf tissue. In a two year field trial, the progressive accumulation of a number of leaf extract constituents (linalool, cubebene, caryophyllene, germacrene D, bicyclogermacrene, cadina-1,4 - diene, aristolone and polygodial) during the growth flush was followed by a slow decline during the subsequent dormant season.
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