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GANPAT UNIVERSITY FACULTY of SCIENCE TEACHING and EXAMINATION SCHEME Programme Bachelor of Science Branch/Spec GANPAT UNIVERSITY FACULTY OF SCIENCE TEACHING AND EXAMINATION SCHEME Programme Bachelor of Science Branch/Spec. Microbiology Semester I Effective from Academic Year 2015- Effective for the batch Admitted in June 2015 16 Teaching scheme Examination scheme (Marks) Subject Subject Name Credit Hours (per week) Theory Practical Code Lecture(DT) Practical(Lab.) Lecture(DT) Practical(Lab.) CE SEE Total CE SEE Total L TU Total P TW Total L TU Total P TW Total UMBA101FOM FUNDAMENTALS 04 - 04 - - - 04 - 04 - - - 40 60 100 - - - OF MICROBIOLOGY UCHA101GCH GENERAL 04 - 04 - - - 04 - 04 - - - 40 60 100 - - - CHEMISTRY-I UPHA101GPH GENERAL 04 - 04 - - - 04 - 04 - - - 40 60 100 - - - PHYSICS-I UENA101ENG ENGLISH-I 02 - 02 - - - 02 - 02 - - - 40 60 100 - - - OPEN SUBJECT – 1 02 - 02 - - - 02 - 02 - - - 40 60 100 - - - UPMA101PRA PRACTICAL - - - 02 - 02 - - - 04 - 04 - - - - 50 50 MODULE-I UPCA101PRA PRACTICAL - - - 02 - 02 - - - 04 - 04 - - - - 50 50 MODULE-I UPPA101PRA PRACTICAL - - - 02 - 02 - - - 04 - 04 - - - - 50 50 MODULE-I Total 16 - 16 06 - 06 16 - 16 12 - 12 200 300 500 - 150 150 GANPAT UNIVERSITY FACULTY OF SCIENCE Programme Bachelor of Science Branch/Spec. Microbiology Semester I Version 1.0.1.0 Effective from Academic Year 2015-16 Effective for the batch Admitted in July 2015 Subject code UMBA 101 Subject Name FUNDAMENTALS OF MICROBIOLOGY FOM Teaching scheme Examination scheme (Marks) (Per week) Lecture(DT) Practical(Lab.) Total CE SEE Total L TU P TW Credit 04 -- -- -- 04 Theory 40 60 100 Hours 04 -- -- -- 04 Practical -- -- -- Pre-requisites: Students should have basic knowledge of Microorganisms and microscopy of 10+2 level. Learning Outcome: The course will help the student to understand basic fundamentals of Microbiology and history of Microbiology. The basic fundamentals include various techniques related to microbes, their maintenance and control. Theory syllabus Unit Content Hrs 1 1.1. Discovery of Microbial World: Theories of Biogenesis and Abiogenesis; Discovery of virus. 15 Discovery and developments of vaccines and modern chemotherapy. 1.2. Contributions of scientists in the field of microbiology: Anton van Leeuwenhoek, Robert Koch, Iwanowsky, Winogradsky, Beijerinck, Alexander Fleming, Selman A. Waksmann, Paul Ehrlich and Golden era of Microbiology. 1.3. Importance and applications of Microbiology in various fields. 1.4. Establishment of germ theory of diseases and fermentation. Work of Lister and principles of aseptic surgery. 2 MICROBIOLOGICAL TECHNIQUES ‐ I 15 2.1. Sterilization and disinfection techniques: Principles and methods of sterilization. 2.2. Physical: Hot Air Oven, Pressure Cooker, Autoclave, Laminar Air Flow. 2.3. Chemical: Alcohol, Aldehyde, Phenol, Halogen, Hypochlorite, Fumigants, phenol coefficient. 2.4. Radiation methods: UV rays, gamma rays, ultrasonic methods. 3 MICROBIOLOGICAL TECHNIQUES ‐ II 15 3.1. Types of Stains. Definition, acidic basic dyes and leuco compounds. Types of staining techniques: Simple, Differential, Negative, Structural Stains (Gram’s, Spore, Capsule, Cell Wall, Metachromatic etc.). 3.2. Principles of Microscopy: magnification and resolving power. Light microscopy: simple and compound microscope. Bright field and dark field microscopy. 3.3. Principles and application of phase contrast and fluorescent microscopy. 3.4. Electron microscopy: general principles. Types of electron microscopy, their principles working and limitations. 4 PURE CULTURE ISOLATION AND PRESERVATION OF CULTURES 15 4.1. Pure culture techniques – Definition: Pure culture and axenic culture, Principles and methods of obtaining pure culture Enrichment culture, dilution plating, streak plate, spread plate and pour plate techniques, Micromanipulator. 4.2. Preservation of pure culture: Sub culturing, Oil overlay, Sand cultures, Storage at low temperature, Lyophilization, Liquid Nitrogen. 4.3. Microbiological Media: Simple, Complex, Defined, Differential, Selective, Special. 4.4. Role of culture collection centers. Text Books 1 Pelczar, M.J., Chan, E.C.S. and N.R. Kreig (1993). “Microbiology” 5th Edition, Tata Mc Graw Hill Publishing co. Ltd., New Delhi. 2 Dubey, R. C. and D. K. Maheshwari (2000). “General Microbiology”. S. Chand, New Delhi. Reference Books 1 Prescott, M. J., Harley, J.P. and D.A. Klein (2002). “Microbiology”, 5th Edition, WCB Mc GrawHill, New York. 2 Stanier, R.Y., Adelberg, E.A. and J.I. Ingram. (1991). “General Microbiology”, 5th Edition, Printice Hall of India Pvt. Ltd., New Delhi. 3 Black, J. G. (2005). “Microbiology : Principles and Explorations”. John Wiley, U.S.A. 4 Tortora, G.J., Funke, B. R. and C. L. Case (2004). “ Microbiology : An Introduction”, Pearson Education, Singapore. 5 A.S. Rao (1997). “ Introduction to Microbiology”. Printice‐Hall of India Pvt. Ltd., New Delhi. 6 Madigan M.T., Marinkl, J.M and J. Parkar(2000). “Brock Biology of Microorganisms”, 9th Edition, MacMillan Press, England. 7 Frobisher, H., Hinsdil, R.D., Crabtree, K. T. and D. R. Goodhert (2005). “ Fundamentals of Microbiology”, Saunder & Co., London. Note: Version 1.0.0.0 (First Digit= New syllabus/Revision in Full Syllabus, Second Digit=Revision in Teaching Scheme, Third Digit=Revision in Exam Scheme, Forth Digit= Content Revision) L=Lecture, TU=Tutorial, P= Practical/Lab., TW= Term work, DT= Direct Teaching, Lab.= Laboratory work CE= Continuous Evaluation, SEE= Semester End Examination. GANPAT UNIVERSITY FACULTY OF SCIENCE Programme Bachelor of Science Branch/Spec. Microbiology Semester I Version 1.0.1.0 Effective from Academic Year 2015-16 Effective for the batch Admitted in July 2015 Subject code UPMA 101 PRA Subject Name PRACTICAL MODULE - I Teaching scheme Examination scheme (Marks) (Per week) Lecture(DT) Practical(Lab.) Total CE SEE Total L TU P TW Credit -- -- 02 -- 02 Theory -- -- -- Hours -- -- 04 -- 04 Practical -- 50 50 Pre-requisites: Students should have basic knowledge of Microorganisms and microscopy of 10+2 level. Learning Outcome: The course will help the student to understand routine microbiological workout and various staining techniques. Practical contents Unit Content Hrs 1 Safety precautions to work in Microbiology Laboratory. 3 2 Study of principles and working of laboratory instruments Light microscope, Autoclave, Hot air 3 oven, Incubator, Bacteriological filter, Rotary shaker, pH meter, Spectrophotometer, Centrifuge. 3 Cleaning and preparation of glass ware for sterilization. 3 4 Preparation of culture media: Solid & Liquid. 3 5 Sterilization techniques: Autoclaving, hot air oven and filtration. 3 6 Isolation of single colony on solid media. 3 7 Enumeration of bacterial numbers by serial dilution and plating. 3 8 Light & compound microscopes and their handling. 3 9 Microscopic observation of bacteria for Morphological characters. 3 10 Calibrations of microscopic measurements (Ocular & stage micro-meters). 3 11 Simple and differential staining (Gram staining), negative staining. 3 12 Preservation of bacterial cultures. 3 Text Books 1 Experimental microbiology Vol. 1, Rakesh Patel. Note: Version 1.0.0.0 (First Digit= New syllabus/Revision in Full Syllabus, Second Digit=Revision in Teaching Scheme, Third Digit=Revision in Exam Scheme, Forth Digit= Content Revision) L=Lecture, TU=Tutorial, P= Practical/Lab., TW= Term work, DT= Direct Teaching, Lab.= Laboratory work CE= Continuous Evaluation, SEE= Semester End Examination. GANPAT UNIVERSITY FACULTY OF SCIENCE Programme Bachelor of SCIENCE Branch/Spec. MICROBIOLOGY Semester I Version 1.0.1.0 Effective from Academic Year 2015-16 Effective for the batch Admitted in July 2015 Subject code UCHA 101 GCH Subject Name GENERAL CHEMISTRY-I Teaching scheme Examination scheme (Marks) (Per week) Lecture(DT) Practical(Lab.) Total CE SEE Total L TU P TW Credit 04 -- -- -- 04 Theory 40 60 100 Hours 04 -- -- -- 04 Practical -- -- -- Pre-requisites: Students should have basic knowledge of chemistry up to 10+2 level. Learning Outcome: Basic knowledge of valence bond theory and its applications. Knowledge of f-block elements and their periodic properties. Understanding of the concept of lanthanide contraction. Understanding of the concepts of general organic chemistry. Knowledge of reactions and mechanisms in organic chemistry. Basic concepts and theories of thermodynamic chemistry. Introduction of analytical chemistry and related terms. Theory syllabus Unit Content Hrs 1 1.1 CHEMICAL BONDING Valence bond theory. Application of valancebond theory. Directional characteristics of covalent bond (s-s, s-p and p-p). Various types of hybridization and shape of simple inorganic molecules V.S.E.P.R. theory for NH3, H2O, CH4 . M.O. Theory-Energy level diagram for homo nucleus diatomic molecules (N2 and O2) and hetero diatomic molecule (CO and NO) 1.2 F–BLOCK ELEMENTS History of Lanthanides. Minerals of lanthanides. Electronic configuration of lanthanides. Oxidation state of lanthanides. Color of lanthanides. Magnetic properties of lanthanides. Lanthanide contraction, Effect of lanthanide contraction Separation method (1)Solvent extraction methods (2)Ion Exchange Method 02 2.1 STRUCTURE AND PROPERTIES Factors affecting to the properties of organic molecule Intermolecular forces Dipol-dipol interaction Vander waals forces Intramolecular forces Electromeric effect Inductive effect Resonance effect (draw resonating structures of Nitro benzene, Chlorobenzene, Phenoxide ion, Anillinium ion, Acetate ion) Hyperconjugation 2.2 REACTION MECHANISM Fission of Co-Valent bond ( With atleast one reaction as a example of each intermediates ) 1. Types of reagents. 2.
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