Hindawi BioMed Research International Volume 2021, Article ID 5544264, 17 pages https://doi.org/10.1155/2021/5544264 Research Article Marker Genes Change of Synovial Fibroblasts in Rheumatoid Arthritis Patients Lifen Liao,1 Ke Liang,2 Lan Lan,1 Jinheng Wang,1 and Jun Guo 3 1Department of Laboratory, Affiliated Hospital of Guilin Medical University, Xiufeng District, Guilin, 541001 Guangxi, China 2Department of Laboratory, Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Xiangshan District, Guilin, 541002 Guangxi, China 3Department of Hematology, Rizhao People’s Hospital, Donggang District, Rizhao, 276800 Shandong, China Correspondence should be addressed to Jun Guo;
[email protected] Received 27 February 2021; Revised 14 May 2021; Accepted 24 May 2021; Published 7 June 2021 Academic Editor: Tao Huang Copyright © 2021 Lifen Liao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Rheumatoid arthritis (RA) is a chronic condition that manifests as inflammation of synovial joints, leading to joint destruction and deformity. Methods. We identified single-cell RNA-seq data of synovial fibroblasts from RA and osteoarthritis (OA) patients in GSE109449 dataset. RA- and OA-specific cellular subpopulations were identified, and enrichment analysis was performed. Further, key genes for RA and OA were obtained by combined analysis with differentially expressed genes (DEGs) between RA and OA in GSE56409 dataset. The diagnostic role of key genes for RA was predicted using receiver operating characteristic (ROC) curve. Finally, we identified differences in immune cell infiltration between RA and OA patients, and utilized flow cytometry, qRT-PCR, and Western blot were used to examine the immune cell and key genes in RA patients.