Biodiv. Res. Conserv. 29: 1-23, 2013 BRC www.brc.amu.edu.pl DOI 10.2478/biorc-2013-0004 Submitted 27.01.2013, Accepted 31.03.2013 Reclassification of theAngraecum -alliance (Orchidaceae, Vandoideae) based on molecular and morphological data Dariusz L. Szlachetko*, Piotr Tukałło, Joanna Mytnik-Ejsmont & Elżbieta Grochocka Department of Plant Taxonomy and Nature Conservation, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland * corresponding author (e-mail:
[email protected]) Abstract: Results of molecular analysis compared with morphological studies were used for reclassification of theAngraecum - alliance (Orchidaceae). For the purpose of this study we sequenced the ITS region (ITS1-5.8S-ITS2) of nrDNA representing nuclear genome and the plastid region trnL-F (including intron of trnL gene and trnL-trnF intergenic spacer). The ITS matrix includes 97 samples representing 86 species and the trnL-F matrix includes 94 samples representing 86 species. We focus mainly on the genus Angraecum, however the other genera of Angraecinae are also included (Aeranthes, Campylocentrum, Dendrophylax, Cryptopus, Calyptrochilum, Lemurorchis, Jumellea, Neobathiea, Oeonia, Oeoniella, Sobennikoffia). Additional 43 sequences, including an outgroup (Polystachya modesta) and other representatives of the subtribes Aeridinae (Aerides) and Aerangidinae (Aerangis, Angraecopsis, Erasanthe, Solenangis), were obtained from NCBI resources. Bayesian analysis using MrBayes 3.1.2 on the combined ITS/trnL-F matrix were performed. The monophyly of Angraecinae with an inclusion of Aerangidinae is highly supported by both methods (93 BP/100 PP). The Angraecoid taxa formed two well supported clades, namely clade I (89 BP/100 PP) and clade II (84 BP/100 PP). New classification based on both molecular and classical tax- onomy studies is presented including a key to the genera.