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A review International Journal of Sciences, Vol. 4 Issue 1 : 321-327 (January to June, 2009) Studies on tissue culture in KUNDAN KISHOR RAJAK AND HARSH KUMAR

Accepted : December, 2008

Key words : Tissue culture, Sapindaceae, In vitro, Micropropagation, Litchi

apindaceae is a family of flowering found in crop of North Bihar, is a member of the family. The Stemperate to tropical regions throughout the world. It members of the family have not been much exposed to includes many species of economically important tissue culture study. A compilation of tissue culture namely litchi, , pitomba, korlan, , responses of important members of Sapindaceae including mamoncillo, pulasan and ackee. Many species are the important fruit litchi has been attempted. lactiferous containing milky sap and saponins with shoap like qualities and yield products including guarana, Characteristics of sapindaceae: soapberries and maple syrup. Some species of maple and The members of the family Sapindaceae have many buckeye yield valuable , while others are popular common characters. The are normally alternate, ornamentals such as species of Koelreuteria, mostly pinately or palmately, sometimes single compound. Cardiospermum, , Xanthoceras and are usually unisexual, borne in racemes, panicles Ungnadia. Indian macassar oil is product of or corymbs. There are usually four to five free, sometimes Schlerichera trijuga. The family members have not been fused sepals and four to five (often with hairs), much exposed to tissue culture studies. Shoot tip culture which may be absent, and a well-marked disc between of longan cultivars resulted in multiple shoot formation the petals and stamens. There are eight to ten stamens in and regeneration of virus free plants. Callus formation two whorls. The filaments are free and often hairy. The and somatic embryogenesis were observed from the ovary is superior with two to four lobes, and the style is cultured and zygotic embryo explants of ackee. Tissue simple or divided. The fruits include capsules, nuts, berries, culture of litchi resulted in callus formation, somatic drupes, samaras or schizocarps, often red, containing embryogenesis, multiple shoot formation, plant . They lack endosperm, with the embryo folded or regeneration from different cultured explants including curved. , leaf, stem, shoot bud, anther and protoplast. Micropropagation techniques were developed for Important plants of sapindaceae: guarana, litchi, longan and rambutan. Many members of Sapindaceae are important plants The Sapindaceae is a family of flowering plants. The having broad range of uses. Some plants contain saponin species are found in temperate to tropical regions in their fruit, seed and other tissues. Sapindus saponaria throughout the world, but are widely distributed in warm and Sapindus trifoliatus are used as soap substitutes. sub-tropics and tropics. Most of the species are native of Schleichera trijuga is the source of macassar oil, used Asia but some have their origin in South America, in ointments. Maple syrup is yet another commercial and Australia. The family consists of more than 150 genera product. Paullinia cupana is a plant and is the source and 2000 species of mostly and , but rarely of guarana, a soft drink used in many countries. herbs also. The family includes unique un-branched palm Some members of the family are important timber like such as Talisia and woody climbers namely trees such as species of maple and buckeye, while others Sejania and Paullinia. Many of the largest trees grow are popular ornamentals. Among ornamentals Sapindus up to the height of more than 60m such as Schleschesa saponaria, Koelreuteria paniculata are small trees. oleasa and Pometia pinata. Litchi, the most important Xanthoceras sorbifolia is used for its attractive . Other ornamental plants are Ungnadia speciosa and Correspondence to: Cardiospermum halicacabum. KUNDAN KISHOR RAJAK, Department of Agricultural Biotechnology and Molecular Biology, Faculty of Basic Many economically important fruit plants are also Sciences and Humanities, Rajendra Agricultural University, included in this family namely sapida, ackee, a Pusa, SAMASTIPUR (BIHAR) fruit with edible ; Melicocca bijuga, mamoncillo or Authors’ affiliations: HARSH KUMAR, Department of Agricultural Spanish lime and Pometia pinnata, with an edible aril. Biotechnology and Molecular Biology, Faculty of Basic Other fruit species of the family worth mentioning are Sciences and Humanities, Rajendra Agricultural University, Pusa, SAMASTIPUR (BIHAR) Cubilia bancoi, Kubili; Diploglottis cunninghamii,

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