Genome Size Variation in a Hybrid Zone Between Homoploid Species in Anacyclus L
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Genome size variation in a hybrid zone between homoploid species in Anacyclus L. (Anthemideae, Asteraceae)
Presentation · November 2015 DOI: 10.13140/RG.2.2.10967.98727
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Bruno Agudo Rubén Torices King Juan Carlos University 20 PUBLICATIONS 29 CITATIONS 52 PUBLICATIONS 647 CITATIONS SEE PROFILE SEE PROFILE
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The user has requested enhancement of the downloaded file. Genome size variation in a hybrid zone between homoploid species in Anacyclus L. (Anthemideae, Asteraceae)
Alicia Agudo1, Rubén Torices2,3,4, João Loureiro4, Sílvia Castro4, Mariana Castro4 and Inés Álvarez1
The ADN The Genome The complete set of genes
only coding regions The Genome The complete set of genes
only coding regions
The total genetic content in a set of chromosomes coding regions + non-coding regions The Genome The complete set of genes
only coding regions
The total genetic content in a set of chromosomes coding regions + non-coding regions Genome Size
Gregory TR. 2004 Genome Size
Gregory TR. 2004 Genome Size
Gregory TR. 2004 Genome Size
Gregory TR. 2004 Genome Size
Gregory TR. 2004 Gregory TR. 2005 There are lots of interesting factors and processus concerning the genome size we could discuss about, but…
Gregory TR. 2005 There are lots of interesting factors and processus concerning the genome size we could discuss about, but…
Gregory TR. 2005 Genome keeps a constant size per species Genome Size Databases
( Genome keeps a constant size per species ) Genome Size Databases
( Genome keeps a constant size per species ) And we found constant sizes per species in Anacyclus too, but with a high range of variation in some cases And we found constant sizes per species in Anacyclus too, but with a high range of variation in some cases
Results And we found constant sizes per species in Anacyclus too, but with a high range of variation in some cases
(But these are already Results) Hybrid Zone
Anacyclus clavatus Anacyclus valentinus Distribution areas and sampling
A. clavatus
A. valentinus
A. homogamos Overlapping distribution areas
Where these species encounter and hybrid populations can be found A. clavatus
A. valentinus
A. homogamos Material & Methods
Sympatric populations analysed
A. clavatus
A. valentinus
A. homogamos
10 86 34 42 individuals / pop. Material & Methods
Populations where the donors for the experimental crosses came from (1 pop. /species)
A. clavatus
A. valentinus
A. homogamos Material & Methods
Genome size estimations using flow cytometry (FCM)
Nuclei were stained with propidium iodide (PI)
Histogram of nuclear DNA content
Flow cytometer Results
CxH F1 hybirds HxV CxV
Intrasp. crosses
V C H Results “Pure” populations Results
A. clavatus from overlapping areas Iberian Peninsula and Morocco Results
Sympatric populations v i c Results
Experimental crosses CxV HxV CxH Results Results Results Results Results Conclusions
Anacyclus hybrids show intermediate values between the parental species intercrossed (usually coincident with the arithmetical average)
Coincidences in genome size values of individuals from overlapping areas between A. clavatus and A. valentinus and experimental hybrids of these species support the hypothesis of a hybridization process taking place
The range of variation of A. clavatus outside overlapping areas is low, and it increases when individuals from overlapping areas are sampled
A high range of variation in the genome size of a species points out that gene flow might be occuring
Hybrids can express a “pure phenotypes” (we cannot trust them)
The genome size analysis is a useful and simple technique to characterize populations or species and experiment design
THANK YOU!
This study makes part of the proyect Analysis of the A. clavatus (Desf.) Pers. and A. valentinus L. contact zone”
With the financial support of the Ministerio de Economía y Competitividad (Ministry of Economy and Competiveness) for the project (CGL2010-18039) and my predoctoral grant FPI (BES-2011- 048197)
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