PKC Alpha Rabbit Pab

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Leader in Biomolecular Solutions for Life Science PKC alpha Rabbit pAb Catalog No.: A13342 Basic Information Background Catalog No. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that A13342 can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved Observed MW in diverse cellular signaling pathways. PKC family members also serve as major 85kDa receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The Calculated MW protein encoded by this gene is one of the PKC family members. This kinase has been 76kDa reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice Category suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. Primary antibody Applications WB Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 5578 P17252 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 523-672 of human PKC alpha (NP_002728.1). Synonyms PRKCA;AAG6;PKC-alpha;PKCA;PRKACA;PKC alpha Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using PKC alpha antibody (A13342) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Anti-PRKCA / PKC-Alpha Antibody (Aa623-672) Rabbit Anti Human Polyclonal Antibody Catalog # ALS17942
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Anti-PRKCA / PKC-Alpha Antibody (aa623-672) Rabbit Anti Human Polyclonal Antibody Catalog # ALS17942 Specification Anti-PRKCA / PKC-Alpha Antibody (aa623-672) - Product Information Application IHC-P, E Primary Accession P17252 Predicted Human, Mouse, Rat Host Rabbit Clonality Polyclonal Isotype IgG Calculated MW 76750 Anti-PRKCA / PKC-Alpha Antibody (aa623-672) - Additional Information Gene ID 5578 Alias Symbol PRKCA Other Names PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha Target/Specificity PRKCA Antibody detects endogenous levels of total PRKCA protein. Reconstitution & Storage Immunoaffinity purified Precautions Anti-PRKCA / PKC-Alpha Antibody (aa623-672) is for research use only and not for use in diagnostic or therapeutic procedures. Anti-PRKCA / PKC-Alpha Antibody (aa623-672) - Protein Information Name PRKCA Synonyms PKCA, PRKACA Function Page 1/4 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Calcium-activated, phospholipid- and diacylglycerol (DAG)- dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells.
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AGC Kinases in Mtor Signaling, in Mike Hall and Fuyuhiko Tamanoi: the Enzymes, Vol
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