Vegetable Peptone Agar

VEGETABLE PEPTONE AGAR

INTENDED USE Remel Vegetable Peptone Agar is a solid medium recommended for use in qualitative procedures for the isolation and cultivation of a wide variety of microorganisms and for sterility testing.

SUMMARY AND EXPLANATION Vegetable Peptone Agar is made from raw materials free from animal-derived products and provides a meat-free alternative to tryptic soy broth. Epidemiological studies have suggested, cattle feed prepared from bovine tissues, specifically brain and spinal cord tissues may be sources of Bovine Spongiform Encephalopathy (BSE).1 Culture media prepared from raw materials free of animal-derived products is recommended for use in the pharmaceutical industry.2,3

PRINCIPLE Vegetable Peptone Agar is an excellent nutritional base for the growth of a wide range of organisms, including many fastidious species. Vegetable peptone provides nitrogenous compounds and amino acids essential for the growth of bacteria. Sodium chloride is a source of essential electrolytes and maintains osmotic equilibrium. Agar is a solidifying agent.

REAGENTS (CLASSICAL FORMULA)* Vegetable Peptone...... 20.0 g Sodium Chloride...... 5.0 g Agar...... 15.0 g pH 7.1 ± 0.2 @ 25°C

*Adjusted as required to meet performance standards.

PRECAUTIONS This product is For Laboratory Use only. It is not intended for use in the diagnosis of disease or other conditions.

PREPARATION OF DEHYDRATED CULTURE MEDIA 1. Suspend 40 g of medium in 1000 ml of demineralized water. 2. Heat with agitation to completely dissolve. 3. Sterilize by autoclaving for 15 minutes at 121°C or following established laboratory procedures. 4. Dispense into appropriate containers.

PROCEDURE 1. Consult current editions of appropriate references for the recommended procedure for sample preparation, inoculation, testing, and interpretation.

QUALITY CONTROL Each lot number of Vegetable Peptone Agar has been manufactured, packaged, and processed in accordance with current Good Manufacturing Practice regulations. All lot numbers have been tested using the following quality control organisms and have been found to be acceptable. Testing of control organisms should be performed in accordance with established laboratory quality control procedures. If aberrant quality control results are noted, sample results should not be reported.

CONTROL INCUBATION RESULTS Aspergillus brasiliensis ATCC® 16404 Ambient, up to 5 days @ 20-25°C Good growth Bacillus subtilis ATCC® 6633 Ambient, up to 5 days @ 20-25°C Good growth Candida albicans ATCC® 10231 Ambient, up to 5 days @ 20-25°C Good growth Enterococcus faecalis ATCC® 29212 Ambient, 18-24 h @ 33-37°C Good growth Escherichia coli ATCC® 8739 Ambient, up to 5 days @ 20-25°C Good growth Escherichia coli ATCC® 25922 Ambient, 18-24 h @ 33-37°C Good growth Listeria monocytogenes ATCC® 7646 Ambient, 18-24 h @ 33-37°C Good growth Pasteurella multocida ATCC® 43137 Ambient, 18-24 h @ 33-37°C Good growth Pseudomonas aeruginosa ATCC® 9027 Ambient, up to 5 days @ 20-25°C Good growth Pseudomonas aeruginosa ATCC® 27853 Ambient, 18-24 h @ 33-37°C Good growth Shigella sonnei ATCC® 9290 Ambient, 18-24 h @ 33-37°C Good growth Staphylococcus aureus ATCC® 6538 Ambient, up to 5 days @ 20-25°C Good growth Staphylococcus aureus ATCC® 25923 Ambient, 18-24 h @ 33-37°C Good growth Streptococcus agalactiae ATCC® 12386 Ambient, 18-24 h @ 33-37°C Good growth ® Streptococcus pneumoniae ATCC 6305 CO2, 18-24 h @ 33-37°C Good growth Streptococcus pyogenes ATCC® 19615 Ambient, 18-24 h @ 33-37°C Good growth

BIBLIOGRAPHY 1. World Health Organization. 2006. Retrieved on August 18, 2010 from: Health, Hazards and Public Debate: Lessons for risk communication from the BSE/CJD saga, Edited by Carlos Dora. http://whqlibdoc.who.int/euro/2006/9289010703_eng.pdf. 2. The United States Pharmacopeia. 2009. 32nd ed. United States Pharmacopeial Convention, Rockville, MD. 3. The European Pharmacopoeia. 2002 4th ed. European Department for the Quality of Medicines, Strasbourg, France.

Refer to the front of Remel Technical Manual of Microbiological Media for General Information regarding precautions, product storage and deterioration, sample collection, storage and transportation, materials required, quality control, and limitations.

ATCC is a registered trademark of American Type Culture Collection.

IFU 455262, Revised September 30, 2010 Printed in U.S.A.

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