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اندام زایی مستقیم از ریزنمونههای بخشهای رویشی L. Gerbera Jamesonii رقم
گل و گیاهان زینتی )1399(، 5 )1(: 1 - 12 مقاله پژوهشی Nilo Gerbera jamesonii L. اندام زایی مستقیم از ریزنمونههای بخشهای رویشی رقم *2 *1 1 مهدی ایزدی ، نیما احمدی ، پژمان آزادی 1. گروه علوم باغبانی دانشکده کشاورزی دانشگاه تربیت مدرس، تهران 2. بخش مهندسی ژنتیک و ایمنی زیستی، پژوهشگاه بیوتکنولوژی کشاورزی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج [email protected] تاریخ دریافت: 20/8/98، تاریخ پذیرش: 1400/2/13 چکیده برای موفقیت استفاده از فنون انتقال ژن، دستیابی به روش کارآمد باززایی ضروری است. در این پژوهش در آزمایش نخست، اندام زایی مستقیم ژربرا از ریزنمونههای برگ کامل، دمبرگ، دمبرگ خراشدار و ﻻیه یاخته ای نازک )Thin Cell ،TCL Layer( و ترکیب تنظیم کننده رشد BAP )0، 1، 2 و 3 میلی گرم در لیتر(، TDZ ) 0، 1/0، 5/0، 8/0 و 1 میلی گرم در لیتر( و IAA با غلظت 1/0 میلی گرم در لیتر در دو حالت نوری )تاریکی به مدت یک ماه و 16 ساعت روشنایی/8 ساعت تاریکی( مورد ارزیابی قرار گرفت. همچنین در آزمایش دوم، باززایی سه برش اول ریزنمونه ﻻیه یاختهای نازک از انتهای دمبرگ متصل به گیاه در ترکیب هورمونی BAP )0، 1 و 2 میلی گرم در لیتر(، TDZ ) 0 و 5/0 میلی گرم در لیتر( و IAA )0، 1/0 و 3/0 میلی گرم در لیتر( مورد ارزیابی قرار گرفت. در تمام آزمایش ها، محیط کشت MS استفاده شد. نتیجهها نشان داد که بهترین محیط باززایی برای ریزنمونههای برگ کامل )2/33% باززایی، 66/3 شاخه(، دمبرگ ) 66/86% باززایی، 33/33 شاخه(، دمبرگ خراش دار )2/53% باززایی، 33/10 شاخه( محیط دارای 2 میلی گرم در لیتر BAP در روشنایی و برای ریزنمونه TCL Downloaded from flowerjournal.ir at 16:56 +0330 on Tuesday September 28th 2021 )6/46% باززایی، 29 شاخه(، 2 میلی گرم در لیتر BAP به همراه 5/0 میلی گرم در لیتر TDZ در تاریکی میباشد. -
Phytosociology of the Upper Orange River Valley, South Africa
PHYTOSOCIOLOGY OF THE UPPER ORANGE RIVER VALLEY, SOUTH AFRICA A SYNTAXONOMICAL AND SYNECOLOGICAL STUDY M.J.A.WERGER PROMOTOR: Prof. Dr. V. WESTHOFF PHYTOSOCIOLOGY OF THE UPPER ORANGE RIVER VALLEY, SOUTH AFRICA A SYNTAXONOMICAL AND SYNECOLOGICAL STUDY PROEFSCHRIFT TER VERKRUGING VAN DE GRAAD VAN DOCTOR IN DE WISKUNDE EN NATUURWETENSCHAPPEN AAN DE KATHOLIEKE UNIVERSITEIT TE NIJMEGEN, OP GEZAG VAN DE RECTOR MAGNIFICUS PROF. MR. F J.F.M. DUYNSTEE VOLGENS BESLUIT VAN HET COLLEGE VAN DECANEN IN HET OPENBAAR TE VERDEDIGEN OP 10 MEI 1973 DES NAMIDDAGS TE 4.00 UUR. DOOR MARINUS JOHANNES ANTONIUS WERGER GEBOREN TE ENSCHEDE 1973 V&R PRETORIA aan mijn ouders Frontiepieae: Panorama drawn by R.J. GORDON when he discovered the Orange River at "De Fraaye Schoot" near the present Bethulie, probably on the 23rd December 1777. I. INTRODUCTION When the government of the Republic of South Africa in the early sixties decided to initiate a comprehensive water development scheme of its largest single water resource, the Orange River, this gave rise to a wide range of basic and applied scientific sur veys of that area. The reasons for these surveys were threefold: (1) The huge capital investment on such a water scheme can only be justified economically on a long term basis. Basic to this is that the waterworks be protected, over a long period of time, against inefficiency caused by for example silting. Therefore, management reports of the catchment area should.be produced. (2) In order to enable effective long term planning of the management and use of the natural resources in the area it is necessary to know the state of the local ecosystems before a major change is instituted. -
Die Plantfamilie ASTERACEAE: 6
ISSN 0254-3486 = SA Tydskrif vir Natuurwetenskap en Tegnologie 23, no. 1 & 2 2004 35 Algemene artikel Die plantfamilie ASTERACEAE: 6. Die subfamilie Asteroideae P.P.J. Herman Nasionale Botaniese Instituut, Privaat sak X101, Pretoria, 0001 e-pos: [email protected] UITTREKSEL Die tribusse van die subfamilie Asteroideae word meer volledig in hierdie artikel beskryf. Die genusse wat aan dié tribusse behoort word gelys en hulle verspreiding aangedui. ABSTRACT The plant family Asteraceae: 6. The subfamily Asteroideae. The tribes of the subfamily Asteroideae are described in this article. Genera belonging to the different tribes are listed and their distribution given. INLEIDING Tribus ANTHEMIDEAE Cass. Hierdie artikel is die laaste in die reeks oor die plantfamilie Verteenwoordigers van hierdie tribus is gewoonlik aromaties, Asteraceae.1-5 In die vorige artikel is die klassifikasie bokant byvoorbeeld Artemisia afra (wilde-als), Eriocephalus-soorte, familievlak asook die indeling van die familie Asteraceae in sub- Pentzia-soorte.4 Die feit dat hulle aromaties is, beteken dat hulle families en tribusse bespreek.5 Hierdie artikel handel oor die baie chemiese stowwe bevat. Hierdie stowwe word dikwels subfamilie Asteroideae van die familie Asteraceae, met ’n aangewend vir medisyne (Artemisia) of insekgif (Tanacetum).4 bespreking van die tribusse en die genusse wat aan die verskillende Verder is hulle blaartjies gewoonlik fyn verdeeld en selfs by dié tribusse behoort. Die ‘edelweiss’ wat in die musiekblyspel The met onverdeelde blaartjies, is die blaartjies klein en naaldvormig sound of music besing word, behoort aan die tribus Gnaphalieae (Erica-agtig). Die pappus bestaan gewoonlik uit vry of vergroeide van die subfamilie Asteroideae. -
Artemisia Afra: a Potential Flagship for African Medicinal Plants? ⁎ N.Q
Available online at www.sciencedirect.com South African Journal of Botany 75 (2009) 185–195 www.elsevier.com/locate/sajb Review Artemisia afra: A potential flagship for African medicinal plants? ⁎ N.Q. Liu, F. Van der Kooy , R. Verpoorte Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, PO Box 9502, 2300RA Leiden, The Netherlands Received 11 July 2008; received in revised form 4 November 2008; accepted 6 November 2008 Abstract The genus Artemisia consists of about 500 species, occurring throughout the world. Some very important drug leads have been discovered from this genus, notably artemisinin, the well known anti-malarial drug isolated from the Chinese herb Artemisia annua. The genus is also known for its aromatic nature and hence research has been focussed on the chemical compositions of the volatile secondary metabolites obtained from various Artemisia species. In the southern African region, A. afra is one of the most popular and commonly used herbal medicines. It is used to treat various ailments ranging from coughs and colds to malaria and diabetes. Although it is one of the most popular local herbal medicines, only limited scientific research, mainly focussing on the volatile secondary metabolites content, has been conducted on this species. The aim of this review was therefore to collect all available scientific literature published on A. afra and combine it into this paper. In this review, a general overview will be given on the morphology, taxonomy and geographical distribution of A. afra. The major focus will however be on the secondary metabolites, mainly the volatile secondary metabolites, which have been identified from this species. -
Dube Nontembeko 2019.Pdf (2.959Mb)
UNDERSTANDING THE FITNESS, PREFERENCE AND PERFORMANCE OF SPECIALIST HERBIVORES OF THE SOUTHERN AFRICAN BIOTYPE OF CHROMOLAENA ODORATA (ASTERACEAE), AND IMPACTS ON PHYTOCHEMISTRY AND GROWTH RATE OF THE PLANT By NONTEMBEKO DUBE Submitted in fulfillment of the academic requirement of Doctorate of Philosophy In The Discipline of Entomology School of Life Sciences College of Agriculture, Engineering and Science University of KwaZulu-Natal Pietermaritzburg South Africa 2019 PREFACE The research contained in this thesis was completed by the candidate while based in the Discipline of Entomology, School of Life Sciences of the College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Pietermaritzburg campus, South Africa, under the supervision of Dr Caswell Munyai, Dr Costas Zachariades, Dr Osariyekemwen Uyi and the guidance of Prof Fanie van Heerden. The research was financially supported by the Natural Resource Management Programmes of the Department of Environmental Affairs, and Plant Health and Protection of the Agricultural Research Council. The contents of this work have not been submitted in any form to another university and, except where the work of others is acknowledged in the text, the results reported are due to investigations by the candidate. _________________________ Signed: N. Dube (Candidate) Date: 08 August 2019 __________________________ Signed: C. Munyai (Supervisor) Date: 08August 8, 2019 ________________________________ Signed: C. Zachariades (Co-supervisor) Date: 08 August 2019 _________________________________ -
Genetic Diversity and Evolution in Lactuca L. (Asteraceae)
Genetic diversity and evolution in Lactuca L. (Asteraceae) from phylogeny to molecular breeding Zhen Wei Thesis committee Promotor Prof. Dr M.E. Schranz Professor of Biosystematics Wageningen University Other members Prof. Dr P.C. Struik, Wageningen University Dr N. Kilian, Free University of Berlin, Germany Dr R. van Treuren, Wageningen University Dr M.J.W. Jeuken, Wageningen University This research was conducted under the auspices of the Graduate School of Experimental Plant Sciences. Genetic diversity and evolution in Lactuca L. (Asteraceae) from phylogeny to molecular breeding Zhen Wei Thesis submitted in fulfilment of the requirements for the degree of doctor at Wageningen University by the authority of the Rector Magnificus Prof. Dr A.P.J. Mol, in the presence of the Thesis Committee appointed by the Academic Board to be defended in public on Monday 25 January 2016 at 1.30 p.m. in the Aula. Zhen Wei Genetic diversity and evolution in Lactuca L. (Asteraceae) - from phylogeny to molecular breeding, 210 pages. PhD thesis, Wageningen University, Wageningen, NL (2016) With references, with summary in Dutch and English ISBN 978-94-6257-614-8 Contents Chapter 1 General introduction 7 Chapter 2 Phylogenetic relationships within Lactuca L. (Asteraceae), including African species, based on chloroplast DNA sequence comparisons* 31 Chapter 3 Phylogenetic analysis of Lactuca L. and closely related genera (Asteraceae), using complete chloroplast genomes and nuclear rDNA sequences 99 Chapter 4 A mixed model QTL analysis for salt tolerance in -
Pteronia Camphorata) Page 1 of 9
Research Article Medicinal use and identity of ǀnhora (Pteronia camphorata) Page 1 of 9 San and Nama indigenous knowledge: The case of AUTHORS: nhora (Pteronia camphorata) and its medicinal use Isabel M. Hulley1 ǀ Patricia M. Tilney1 Sandy F. Van Vuuren2 A hitherto unidentified medicinal plant is here identified for the first time as Pteronia camphorata (L.) L., Guy P.P. Kamatou3 an aromatic shrub of the Asteraceae family endemic to the western and southern coastal region of South 1 Janneke M. Nortje1 Africa. The plant was described in this journal by Laidler in 1928 as ‘D/nhora buchu’, and is one of the Alvaro M. Viljoen3 important types of buchu used by the Nama people. We report the traditional medicinal uses among San Ben-Erik Van Wyk1 and Nama people, based on our interviews with rural participants. These include the treatment of colds, influenza, chest ailments and tuberculosis, as well as convulsions, haemorrhoids and inflammation of AFFILIATIONS: the neck. The major and minor chemical compounds of the essential oil that is produced by the plant are 1Department of Botany and Plant identified, together with the site of accumulation of this volatile oil within the leaf. We also investigated the Biotechnology, University of plant’s antimicrobial activity against a selection of a yeast and two Gram-negative and one Gram-positive Johannesburg, Johannesburg, bacteria, all of which are associated with respiratory infections. P. camphorata is scientifically poorly South Africa known but is an important San and Nama traditional remedy. Our study not only prevents the potential loss 2Department of Pharmacy and Pharmacology, University of the of historically important indigenous knowledge, but also provides the first scientific evidence to validate Witwatersrand, Johannesburg, the traditional use of ǀnhora against upper and lower respiratory tract infections, including tuberculosis. -
Opening the Curiosity Box
Opening the curiosity box: Botanical images as sites of transformation for the scientific practices of annotation and display in the seventeenth and eighteenth centuries Karen Stewart Thesis presented in partial fulfilment of the requirement for the degree of Masters of Philosophy, Visual Arts (Illustration) at Stellenbosch University Supervisor: Professor Keith Dietrich MARCH 2007 Stellenbosch University https://scholar.sun.ac.za Declaration: I, the undersigned declare that the work contained in this thesis is my own original work and that I have not previously in its entirety or in part submitted it at any university for a degree. Stellenbosch University https://scholar.sun.ac.za Summary This thesis investigates the hidden narratives of South African botanical images made in the late seventeenth to eighteenth century. Plant collecting and image making was part of early modernist scientific practice of collection and display. These images are examined from postmodern perspectives that treat them as "texts" that validated colonial botanical agendas. Botanical art objectified "nature" enforcing it into a textual code that sanitised it and made it suitable for study by Eurocentric natural philosophers. The impact of particular scientific agendas about "nature" can be linked to the stereotyping and subjugation of both indigenous knowledge systems and women. This thesis considers the impact that the complex historical and socio-political situations of the seventeenth and eighteenth centuries had to bear on the discursive formations associated with the botanical sciences, of which botanical art forms an integral part. The process whereby indigenous knowledge was effectively written out of acceptable botanical practice (a trend that persists today) is evaluated. -
Rife What Seeds Are to the Earth
1'ou say you donJt 6efieve? Wfiat do you caffit when you sow a tiny seedandare convincedthat a pfant wiffgrow? - Elizabeth York- Contents Abstract . , .. vii Declaration .. ,,., , ,........... .. ix Acknowledgements ,, ,, , .. , x Publications from this Thesis ,, , ", .. ,., , xii Patents from this Thesis ,,,'' ,, .. ',. xii Conference Contributions ' xiii Related Publications .................................................... .. xiv List of Figures , xv List of Tables , ,,,. xviii List of Abbreviations ,,, ,, ,,, ,. xix 1 Introduction ,,,, 1 1.1 SMOKE AS A GERMINATION CUE .. ,,,, .. ,,,,, .. , .. , , . , 1 1.2 AIMS AND OBJECTIVES , '.. , , . 1 1.3 GENERAL OVERVIEW ,, " , .. , .. , 2 2 Literature Review ,",,,,", 4 2.1 THE ROLE OF FIRE IN SEED GERMINATION .. ,,,,.,,,,. ,4 2.1.1 Fire in mediterranean-type regions ', .. ,, , , 4 2,1.2 Post-fire regeneration. ,,,, .. , , . , , , , 5 2,1.3 Effects of fire on germination .,,, , , . 7 2,1,3.1 Physical effects of fire on germination .. ,," .. ,.,. 8 2.1,3.2 Chemical effects of fire on germination ., ,, .. ,., 11 2.2 GERMINATION RESPONSES TO SMOKE., , '" ., , 16 2.2.1 The discovery of smoke as a germination cue, ,,., .. , , .. ,, 16 2.2.2 Studies on South African species. ,.,, .. , ,,,,., 17 2.2,3 Studies on Australian species "",., ,"," ".,." 20 2.2.4 StUdies on species from other regions. , ,,.,, 22 2.2.5 Responses of vegetable seeds ., .. ' .. , ,', , , 23 2.2.6 Responses of weed species .. ,,,.,, 24 2.2.7 General comments and considerations ., .. ,,, .. , .. ,,, 25 2.2.7.1 Concentration effects .. ,", ,., 25 2.2.7.2 Experimental considerations ,,,,,,, 26 2.2,7.3 Physiological and environmental effects ,,, .. ,, 27 2.2.8 The interaction of smoke and heat, ,, ,,,,,,, 29 \ 2.3 SOURCES OF SMOKE ., , .. , .. ,, .. ,., .. ,, 35 2.3,1 Chemical components of smoke ,, .. " ,, 35 iii Contents 2.3.2 Methods of smoke treatments 36 2.3.2.1 Aerosol smoke and smoked media . -
Anthemideae Christoph Oberprieler, Sven Himmelreich, Mari Källersjö, Joan Vallès, Linda E
Chapter38 Anthemideae Christoph Oberprieler, Sven Himmelreich, Mari Källersjö, Joan Vallès, Linda E. Watson and Robert Vogt HISTORICAL OVERVIEW The circumscription of Anthemideae remained relatively unchanged since the early artifi cial classifi cation systems According to the most recent generic conspectus of Com- of Lessing (1832), Hoff mann (1890–1894), and Bentham pos itae tribe Anthemideae (Oberprieler et al. 2007a), the (1873), and also in more recent ones (e.g., Reitbrecht 1974; tribe consists of 111 genera and ca. 1800 species. The Heywood and Humphries 1977; Bremer and Humphries main concentrations of members of Anthemideae are in 1993), with Cotula and Ursinia being included in the tribe Central Asia, the Mediterranean region, and southern despite extensive debate (Bentham 1873; Robinson and Africa. Members of the tribe are well known as aromatic Brettell 1973; Heywood and Humphries 1977; Jeff rey plants, and some are utilized for their pharmaceutical 1978; Gadek et al. 1989; Bruhl and Quinn 1990, 1991; and/or pesticidal value (Fig. 38.1). Bremer and Humphries 1993; Kim and Jansen 1995). The tribe Anthemideae was fi rst described by Cassini Subtribal classifi cation, however, has created considerable (1819: 192) as his eleventh tribe of Compositae. In a diffi culties throughout the taxonomic history of the tribe. later publication (Cassini 1823) he divided the tribe into Owing to the artifi ciality of a subtribal classifi cation based two major groups: “Anthémidées-Chrysanthémées” and on the presence vs. absence of paleae, numerous attempts “An thé midées-Prototypes”, based on the absence vs. have been made to develop a more satisfactory taxonomy presence of paleae (receptacular scales). -
Phylogeny of Hinterhubera, Novenia and Related
Louisiana State University LSU Digital Commons LSU Doctoral Dissertations Graduate School 2006 Phylogeny of Hinterhubera, Novenia and related genera based on the nuclear ribosomal (nr) DNA sequence data (Asteraceae: Astereae) Vesna Karaman Louisiana State University and Agricultural and Mechanical College, [email protected] Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_dissertations Recommended Citation Karaman, Vesna, "Phylogeny of Hinterhubera, Novenia and related genera based on the nuclear ribosomal (nr) DNA sequence data (Asteraceae: Astereae)" (2006). LSU Doctoral Dissertations. 2200. https://digitalcommons.lsu.edu/gradschool_dissertations/2200 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Doctoral Dissertations by an authorized graduate school editor of LSU Digital Commons. For more information, please [email protected]. PHYLOGENY OF HINTERHUBERA, NOVENIA AND RELATED GENERA BASED ON THE NUCLEAR RIBOSOMAL (nr) DNA SEQUENCE DATA (ASTERACEAE: ASTEREAE) A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Biological Sciences by Vesna Karaman B.S., University of Kiril and Metodij, 1992 M.S., University of Belgrade, 1997 May 2006 "Treat the earth well: it was not given to you by your parents, it was loaned to you by your children. We do not inherit the Earth from our Ancestors, we borrow it from our Children." Ancient Indian Proverb ii ACKNOWLEDGMENTS I am indebted to many people who have contributed to the work of this dissertation. -
Ecological Impact Assessment Proposed Saldanha Bay Network Strengthening Project, Saldanha Bay Local Municipality, Western Cape Province
ECOLOGICAL IMPACT ASSESSMENT PROPOSED SALDANHA BAY NETWORK STRENGTHENING PROJECT, SALDANHA BAY LOCAL MUNICIPALITY, WESTERN CAPE PROVINCE JANUARY 2017 Prepared by: Prepared for: Afzelia Environmental Consultants Savannah Environmental P.O. Box 37069, Tel: 011 656 3237 Overport, 4067 Fax: 086 684 0547 Tel: 031 303 2835 Fax: 086 692 2547 Email: [email protected] Email: [email protected] Declaration I, Leigh-Ann de Wet, declare that - • I act as an independent specialist in this application; • I do not have and will not have any vested interest (either business, financial, personal or other) in the undertaking of the proposed activity, other than remuneration for work performed in terms of the Environmental Impact Assessment Regulations, 2010 and 2014; • I will perform the work relating to the application in an objective manner, even if this results in views and findings that are not favourable to the applicant; • I declare that there are no circumstances that may compromise my objectivity in performing such work; • I have expertise in conducting the specialist report relevant to this application, including knowledge of the Act, regulations and any guidelines that have relevance to the proposed activity; • I will comply with the Act, regulations and all other applicable legislation; • I have not and will not engage in, conflicting interests in the undertaking of the activity; • I undertake to disclose to the applicant and the competent authority all material information in my possession that reasonably has or may have the potential of influencing any decision to be taken with respect to the application by the competent authority; and the objectivity of any report, plan or document to be prepared by myself for submission to the competent authority; • All the particulars furnished by me in this form are true and correct.