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Volume 24 Number 1 1983 Review Articles S. Kondo: Carcinogenesis in Relation to the Stem-Cell-Mutation Hypothesis 1 Original Articles Y. Kano, S. Natori: Change in Free Amino Acids and Phospholipids in the Head of Adult Sarcophaga peregrina with Age 9 R.T. Moon: Poly(A)-Containing Mesöenger Ribonucleoprotein Complexes from Sea Urchin Eggs and Embryos: Polypeptides Associated with Native and UV-Crosslinked mRNPs 13 H.R. Kobel, J. Wolff: Two Transitions of Haemoglobin Expression in Xenopus: from Embryonic to Larval and from Larval to Adult 24 G. V. Lopashov: Transdifferentiation of Pigmented Epithelium Induced by the Influence of Lens Epithelium in Frogs 27 LH. Errington, D.N. Cooper, R.M. Clayton: The Pattern of DNA Methylation in the <5-Crystallin Genes in Transdifferentiating Neural Retina Cultures 33 S. Tokunaka, T.M. Friedman, Y. Toyama, M. Pacifici, H. Holtzer: Taxol Induces Microtubule-Rough Endoplasmic Reticulum Complexes and Microtubule-Bundles in Cultured Chondroblasts 39 B.K. Grove, F.E. Stockdale: Regulation of Membrane Transport Sites for Amino Acids in Myogenic Cells. A Differentiation Dependent Phenomenon 48 T.-C. Wu, Y.-J. Wan, I. Damjanov: Fluorescein-Conjugated Bandeiraea simplicifolia Lectin as a Marker of Endodermal, Yolk Sac, and Trophoblastic Differentiation in the Mouse Embryo 55 T.D. Friedrich, U. Regenass, L.C. Stevens: Mouse Genital Ridges in Organ Culture: The Effects of Temperature on Maturation and Experimental Induction of Teratocarcinogenesis 60 H. Blüthmann, E. Vogt, P. Hösli, L.C. Stevens, K. Illmensee: Enzyme Activity Profiles in Mouse Teratocarcinomas. A Quantitative Ultramicroscale Analysis 65 P.E. Ball, M.C. Conroy, C.H. Heusser, J.M. Davis, J.-F. Conscience: Spontaneous, in vitro, Malignant Transformation of a Basophil/Mast Cell Line 74 J. Zlatanova, L. Srebreva, R. Tsanev: Possible Artifacts in the Electrophoretic Study of Histone H10 79 Published by Springer International Differentiation 258 ISSN 0301 -4681 DFFNAW 24 (1) 1 -82 (1983) April 1983 Springer Differentiation Founding Editor: DIMITRI VIZA International Editor-in-Chief: WERNER W. FRANKE The authors should consult the latest version of Information for Contributors, printed in the last issue of each volume, to make sure that their manuscript conforms to the style of the Journal and lies within its scope. Manuscripts should be addressed to one of the following editorial offices, or to any member of the Editorial Board. Regional Editorial Office Regional Editorial Office Central Editorial Office (Europe) (North America) KARLILLMENSEE G. BARRY PIERCE WERNER W. FRANKE Ecole de Medecine Department of Pathology Division of Membrane Biology and Biochemistry 20, rue de l'Ecole-de-Medecine University of Colorado Institute of Cell and Tumor Biology CH-1211 Geneva 4, Switzerland Health Sciences Center German Cancer Research Center 4200 East Ninth Avenue Im Neuenheimer Feld 280 Campus Box B 216 Postfach 101949 Denver, CO 80220, USA D-6900 Heidelberg 1 (FRG) Editorial Board R. AUERBACH Madison, WI, USA G. J.V. NOSSAL Victoria, Australia J.T. BONNER Princeton, NJ, USA S. OHNO Duarte, CA, USA J. BRÄCHET Rhode-St.-Genese, Belgium R.M. PRATT Research Triangle Park, NC, USA M. A. DiBERARDINO Philadelphia, PA, USA M. OSBORN Göttingen, Germany (FRG) L. DuPASQUIER Basel, Switzerland T. PUCK Denver, CO, USA G. GIUDICE Palermo, Italy M. RICCI Firenze, Italy H. GRUNZ Essen, Germany (FRG) M. RODBELL Bethesda, MD, USA M. HICKS London, England, U.K. C. ROSENFELD Villejuif (France) B. HOGAN London, England, U.K. A. RUTHMANN Bochum, Germany (FRG) H. HOLTZER Philadelphia, PA, USA T. SACHS Jerusalem, Israel Ph. JEANTEUR Montpellier, France K. SANDER Freiburg, Germany (FRG) H. R. KOBEL Geneva, Switzerland M. SCHACHNER Heidelberg, Germany (FRG) L. G. LAJTHA Manchester, England, U.K. H. SLAVKIN Los Angeles, CA, USA G. V. LOPASHOV Moscow, USSR M. SUSSMAN Pittsburgh, PA, USA P.D. MACLEAN Bethesda, MD, USA D. TARIN Oxford, England, U.K. C. L. MARKERT New Häven, CT, USA S.TOIVONEN Helsinki 10, Finland R.G. McKINNELL St. Paul, MN, USA T. YAMADA Epalinges s. Lausanne, Switzerland F. MEINS, Jr. Basel, Switzerland K. YAMANA Fukuoka, Japan A. MONROY Naples, Italy Assistant Editor: GISELA KROHNE-EHRICH, D ivision of Membrane Biology and Biochemistry, Institute of Cell and Tumor Biology, Feld 280, Postfach 101949, D-6900 Heidelberg 1 (FRG) German Cancer Research Center, Im Neuenheimer Copyright Supscription information It is a fundamental condition that submitted manuscripts have not been Volumes 23 - 24 (3 issues each) will appear in 1983. 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KG Verwertungsgesellschaft WORT, Abteilung Wissenschaft, Goethestraße 49, Berlin • Heidelberg 1983 D-8000 München 2, for detailed information. Printed in Germany Das Heft enthält eine Beilage des Springer-Verlages Berlin Heidelberg New York Tokyo Differentiation The Journal of the International Society of Differentiation Vol. 24 1983 Founding Editor G.V. Lopashov (Moscow) Dimitri Viza (Paris) P.D. MacLean (Bethesda) Editor-in-Chief C.L. Markert (New Häven) R.G. McKinnell (St. Paul, Minn.) W.W. Franke (Heidelberg) F. Meins, Jr. (Basel) A. Monroy (Naples) Regional Editor G. J.V. Nossal (Victoria) G.B. Pierce (Denver) S. Ohno (Duarte) M. Osborn (Göttingen) Editorial Board R.M. Pratt (Research Triangle Park) R. Auerbach (Madison) T. Puck (Denver) J.T. Bonner (Princeton) M. Ricci (Firenze) J. Brächet (Rhode-St. Genese) M. Rodbell (Bethesda) M.A. DiBerardino (Philadelphia) C. Rosenfeld (Villejuif) L. DuPasquier (Basel) A. Ruthmann (Bochum) G. Giudice (Palermo) T. Sachs (Jerusalem) H. 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  • HUMAN CYTOKERATINS Their Use As Targets in Cancer Management By

    HUMAN CYTOKERATINS Their Use As Targets in Cancer Management By

    UMEÅ UNIVERSITY MEDICAL DISSERTATIONS New series No 284 — ISSN 0346-6612 From the Department of Medical Biochemistry and Biophysics University of Umeå, Umeå, Sweden HUMAN CYTOKERATINS their use as targets in cancer management by Birgitta Sundström Umeå 1990 HUMAN CYTOKERATINS their use as targets in cancer management AKADEMISK AVHANDLING som med vederbörigt tillstånd av Rektorsämbetet vid Umeå universitet för avläggande av doktorsexamen i medicinsk vetenskap kommer att offentligen försvaras i sal B, LU 0 (Naturvetarhuset), Umeå universitet, fredagen den 5 oktober 1990, kl. 09.15 av Birgitta Sundström Umeå 1990 ABSTRACT HUMAN CYTOKERATINS - their use as targets in cancer management Birgitta Sundström, Department of Medical Biochemistry and Biophysics, University of Umeå, S 901 87 Umeå, Sweden. Cytokeratins, biochemically related to intermediate filaments (IF), form an intracellular network of filaments which contributes to the mechanical stabilizing of the cell. 19 individual polypeptides, divided into two groups, constitute the cytokeratin family. Each type of epithelial cell can be characterized by its content of cytokeratin polypeptides since the expression pattern varies with the type of epithelium. During the transformation of epithelial cells into tumours, the cytokeratin patterns are usually maintained. This property has enabled cytokeratins to be used as tumour markers, especially for tumours not easily classified. In order to evaluate cytokeratins as tumour markers, we have generated a battery of monoclonal antibodies (MAbs) against cytokeratins extracted from carcinomas. Five antibodies were further characterized. All reacted with cytokeratin 8 (CK 8) amongst others, but only one, TS 1, was specific for this keratin. CK 8 is o ne of the most abundant keratins in carcinomas.