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Evolution and Comparative Morphology of the Euglenophyte
EVOLUTION AND COMPARATIVE MORPHOLOGY OF THE EUGLENOPHYTE PLASTID by PATRICK JERRY PAUL BROWN (Under the Direction of Mark A. Farmer) ABSTRACT My doctoral research centered on understanding the evolution of the euglenophyte protists, with special attention paid to their plastids. The euglenophytes are a widely distributed group of euglenid protists that have acquired a chloroplast via secondary symbiogenesis. The goals of my research were to 1) test the efficacy of plastid morphological and ultrastructural characters in phylogenetic analysis; 2) understand the process of plastid development and partitioning in the euglenophytes; 3) to use a plastid- encoded protein gene to determine a euglenophyte phylogeny; and 4) to perform a multi- gene analysis to uncover clues about the origins of the euglenophyte plastid. My work began with an alpha-taxonomic study that redefined the rare euglenophyte Euglena rustica. This work not only validly circumscribed the species, but also noted novel features of its habitat, cyclic migration habits, and cellular biology. This was followed by a study of plastid morphology and development in a number of diverse euglenophytes. The results of this study showed that the plastids of euglenophytes undergo drastic changes in morphology and ultrastructure over the course of a single cell division cycle. I concluded that there are four main classes of plastid development and partitioning in the euglenophytes, and that the class a given species will use is dependant on its interphase plastid morphology and the rigidity of the cell. The discovery of the class IV partitioning strategy in which cells with only one or very few plastids fragment their plastids prior to cell division was very significant. -
Lateral Gene Transfer of Anion-Conducting Channelrhodopsins Between Green Algae and Giant Viruses
bioRxiv preprint doi: https://doi.org/10.1101/2020.04.15.042127; this version posted April 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 5 Lateral gene transfer of anion-conducting channelrhodopsins between green algae and giant viruses Andrey Rozenberg 1,5, Johannes Oppermann 2,5, Jonas Wietek 2,3, Rodrigo Gaston Fernandez Lahore 2, Ruth-Anne Sandaa 4, Gunnar Bratbak 4, Peter Hegemann 2,6, and Oded 10 Béjà 1,6 1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel. 2Institute for Biology, Experimental Biophysics, Humboldt-Universität zu Berlin, Invalidenstraße 42, Berlin 10115, Germany. 3Present address: Department of Neurobiology, Weizmann 15 Institute of Science, Rehovot 7610001, Israel. 4Department of Biological Sciences, University of Bergen, N-5020 Bergen, Norway. 5These authors contributed equally: Andrey Rozenberg, Johannes Oppermann. 6These authors jointly supervised this work: Peter Hegemann, Oded Béjà. e-mail: [email protected] ; [email protected] 20 ABSTRACT Channelrhodopsins (ChRs) are algal light-gated ion channels widely used as optogenetic tools for manipulating neuronal activity 1,2. Four ChR families are currently known. Green algal 3–5 and cryptophyte 6 cation-conducting ChRs (CCRs), cryptophyte anion-conducting ChRs (ACRs) 7, and the MerMAID ChRs 8. Here we 25 report the discovery of a new family of phylogenetically distinct ChRs encoded by marine giant viruses and acquired from their unicellular green algal prasinophyte hosts. -
Phylogenetic Analysis and Substitution Rate Estimation of Colonial Volvocine Algae Based on Mitochondrial Genomes
G C A T T A C G G C A T genes Article Phylogenetic Analysis and Substitution Rate Estimation of Colonial Volvocine Algae Based on Mitochondrial Genomes Yuxin Hu 1,2, Weiyue Xing 1,2, Zhengyu Hu 3 and Guoxiang Liu 1,* 1 Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; [email protected] (Y.H.); [email protected] (W.X.) 2 School of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China 3 State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; [email protected] * Correspondence: [email protected]; Tel.: +86-027-6878-0576 Received: 11 December 2019; Accepted: 15 January 2020; Published: 20 January 2020 Abstract: We sequenced the mitochondrial genome of six colonial volvocine algae, namely: Pandorina morum, Pandorina colemaniae, Volvulina compacta, Colemanosphaera angeleri, Colemanosphaera charkowiensi, and Yamagishiella unicocca. Previous studies have typically reconstructed the phylogenetic relationship between colonial volvocine algae based on chloroplast or nuclear genes. Here, we explore the validity of phylogenetic analysis based on mitochondrial protein-coding genes. Wefound phylogenetic incongruence of the genera Yamagishiella and Colemanosphaera. In Yamagishiella, the stochastic error and linkage group formed by the mitochondrial protein-coding genes prevent phylogenetic analyses from reflecting the true relationship. In Colemanosphaera, a different reconstruction approach revealed a different phylogenetic relationship. This incongruence may be because of the influence of biological factors, such as incomplete lineage sorting or horizontal gene transfer. We also analyzed the substitution rates in the mitochondrial and chloroplast genomes between colonial volvocine algae. -
Subunit Rrna Genes of the Genus Euglena Ehrenberg
International Journal of Systematic and Evolutionary Microbiology (2001), 51, 773–781 Printed in Great Britain Phylogenetic analysis of chloroplast small- subunit rRNA genes of the genus Euglena Ehrenberg Department of Plant Rafał Milanowski, Boz0 ena Zakrys! and Jan Kwiatowski Systematics and Geography, Warsaw University, Al. Ujazdowskie 4, PL-00-478 ! Warszawa, Poland Author for correspondence: Boz0 ena Zakrys. Tel: j48 22 628 2595. Fax: j48 22 622 6646. e-mail: zakrys!mercury.ci.uw.edu.pl Almost complete sequences of plastid SSU rDNA (16S rDNA) from 17 species ! belonging to the order Euglenales (sensu Nemeth, 1997; Shi et al., 1999) were determined and used to infer phylogenetic relationships between 10 species of Euglena, three of Phacus, and one of each of Colacium, Lepocinclis, Strombomonas, Trachelomonas and Eutreptia. The maximum-parsimony (MP), maximum-likelihood (ML) and distance analyses of the unambiguously aligned sequence fragments imply that the genus Euglena is not monophyletic. Parsimony and distance methods divide Euglenaceae into two sister groups. One comprises of representatives from the subgenera Phacus, Lepocinclis and ! Discoglena (sensu Zakrys, 1986), whereas the other includes members of ! Euglena and Calliglena subgenera (sensu Zakrys, 1986), intermixed with representatives of Colacium, Strombomonas and Trachelomonas. In all analyses subgenera Euglena – together with Euglena polymorpha (representative of the subgenus Calliglena) – and Discoglena – together with Phacus and Lepocinclis – form two well-defined clades. The data clearly indicate that a substantial revision of euglenoid systematics is very much required, nevertheless it must await while more information can be gathered, allowing resolution of outstanding relationships. Keywords: chloroplast SSU rDNA, Euglena, Calliglena, Discoglena, molecular phylogeny INTRODUCTION The genus Euglena consists of organisms highly diversified with respect to cell architecture. -
Phylogenetic Analysis of ''Volvocacae'
Phylogenetic analysis of ‘‘Volvocacae’’ for comparative genetic studies Annette W. Coleman† Division of Biology and Medicine, Brown University, Providence, RI 02912 Edited by Elisabeth Gantt, University of Maryland, College Park, MD, and approved September 28, 1999 (received for review June 30, 1999) Sequence analysis based on multiple isolates representing essen- most of those obtained previously with data for other DNA tially all genera and species of the classic family Volvocaeae has regions in identifying major clades and their relationships. clarified their phylogenetic relationships. Cloned internal tran- However, the expanded taxonomic coverage revealed additional scribed spacer sequences (ITS-1 and ITS-2, flanking the 5.8S gene of and unexpected relationships. the nuclear ribosomal gene cistrons) were aligned, guided by ITS transcript secondary structural features, and subjected to parsi- Materials and Methods mony and neighbor joining distance analysis. Results confirm the The algal isolates that form the basis of this study are listed below notion of a single common ancestor, and Chlamydomonas rein- and Volvocacean taxonomy is summarized in Table 1. The taxon harditii alone among all sequenced green unicells is most similar. names are those found in the culture collection listings. Included Interbreeding isolates were nearest neighbors on the evolutionary is the Culture Collection designation [University of Texas, tree in all cases. Some taxa, at whatever level, prove to be clades National Institute for Environmental Studies (Japan), A.W.C. or by sequence comparisons, but others provide striking exceptions. R. C. Starr collection], an abbreviated name, and the GenBank The morphological species Pandorina morum, known to be wide- accession number. -
"Phycology". In: Encyclopedia of Life Science
Phycology Introductory article Ralph A Lewin, University of California, La Jolla, California, USA Article Contents Michael A Borowitzka, Murdoch University, Perth, Australia . General Features . Uses The study of algae is generally called ‘phycology’, from the Greek word phykos meaning . Noxious Algae ‘seaweed’. Just what algae are is difficult to define, because they belong to many different . Classification and unrelated classes including both prokaryotic and eukaryotic representatives. Broadly . Evolution speaking, the algae comprise all, mainly aquatic, plants that can use light energy to fix carbon from atmospheric CO2 and evolve oxygen, but which are not specialized land doi: 10.1038/npg.els.0004234 plants like mosses, ferns, coniferous trees and flowering plants. This is a negative definition, but it serves its purpose. General Features Algae range in size from microscopic unicells less than 1 mm several species are also of economic importance. Some in diameter to kelps as long as 60 m. They can be found in kinds are consumed as food by humans. These include almost all aqueous or moist habitats; in marine and fresh- the red alga Porphyra (also known as nori or laver), an water environments they are the main photosynthetic or- important ingredient of Japanese foods such as sushi. ganisms. They are also common in soils, salt lakes and hot Other algae commonly eaten in the Orient are the brown springs, and some can grow in snow and on rocks and the algae Laminaria and Undaria and the green algae Caulerpa bark of trees. Most algae normally require light, but some and Monostroma. The new science of molecular biology species can also grow in the dark if a suitable organic carbon has depended largely on the use of algal polysaccharides, source is available for nutrition. -
Identification of Freshwater Invertebrates
Identification of Freshwater Invertebrates © 2008 Pennsylvania Sea Grant To request copies, please contact: Sara Grisé email: [email protected] Table of Contents A. Benthic Macroinvertebrates……………………….………………...........…………1 Arachnida………………………………..………………….............….…2 Bivalvia……………………...…………………….………….........…..…3 Clitellata……………………..………………….………………........…...5 Gastropoda………………………………………………………..............6 Hydrozoa………………………………………………….…………....…8 Insecta……………………..…………………….…………......…..……..9 Malacostraca………………………………………………....…….…....22 Turbellaria…………………………………………….….…..........…… 24 B. Plankton…………………………………………...……….………………............25 Phytoplankton Bacillariophyta……………………..……………………...……….........26 Chlorophyta………………………………………….....…………..........28 Cyanobacteria…...……………………………………………..…….…..32 Gamophyta…………………………………….…………...….…..…….35 Pyrrophycophyta………………………………………………………...36 Zooplankton Arthropoda……………………………………………………………....37 Ciliophora……………………………………………………………......41 Rotifera………………………………………………………………......43 References………………………………………………………….……………….....46 Taxonomy is the science of classifying and naming organisms according to their characteris- tics. All living organisms are classified into seven levels: Kingdom, Phylum, Class, Order, Family, Genus, and Species. This book classifies Benthic Macroinvertebrates by using their Class, Family, Genus, and Species. The Classes are the categories at the top of the page in colored text corresponding to the color of the page. The Family is listed below the common name, and the Genus and Spe- cies names -
Euglenophytes Reported from Karst Sink-Holes in the Malopolska Upland (Poland, Central Europe)
Ann. Limnol. - Int. J. Lim. 39 (4), 333 - 346 Euglenophytes reported from karst sink-holes in the Malopolska Upland (Poland, Central Europe) K. Wolowski Polish Academy of Sciences, W. Szafer Institute of Botany, Department of Phycology, ul. Lubicz 46, PL 31-512 Kraków, Poland. E-mail: [email protected] In karst sink-holes within the environs of Czajków, and in an old fishpond within the Skorocice gypsum valley, 24 taxa of eu- glenophytes, comprising Euglena (10), Phacus (9), Lepocinclis (2), and Trachelomonas (3) were found. Four of them, Phacus elegans Pochmann, Ph. arnoldii Swirenko var. ovatus Popova, Ph. plicatus Conrad, and Ph. curvicauda Swir. var. robusta Al- lorge & Lefèvre, are new for the Polish flora, and rarely reported worldwide. Keywords : Euglenophyta, Central Europe, Poland, Malopolska Upland, karst sink-holes, fishponds. Introduction Material and methods The investigated area lies in the eastern part of the One collection of material was made from each lo- Nida Basin, a wide depression within the ´Swietokrzys-, cation during summer (June 1986). Unfiltered water kie Mountains and Cracow-Czestochowa, Upland. The (containing plankton) along with the bottom of two origin of the karst sink-holes in this area is connected sink-holes at Czajków and two fishponds at Skorocice to the occurrence of varying ground water levels. The were sampled. The water temperature in the sink-holes highest level lies just below the surface of Quaternary at Czajków was 17°C and the pH value was 5.4 (area formation and the lowest is situated in the Tertiary ca. = 0.80 ha, depth = 40 cm). -
Studies on the Influence of Microcystis Aeruginosa on the Ecology and Fish Production of Carp Culture Ponds
African Journal of Biotechnology Vol. 8 (9), pp. 1911-1918, 4 May, 2009 Available online at http://www.academicjournals.org/AJB ISSN 1684–5315 © 2009 Academic Journals Full Length Research Paper Studies on the influence of Microcystis aeruginosa on the ecology and fish production of carp culture ponds P. Padmavathi* and K. Veeraiah Department of Zoology, Acharya Nagarjuna University, Nagarjuna Nagar - 522 510, A.P., India. Accepted 26 December, 2008 In many fish ponds, blue-green algae (Cyanobacteria) constitute the greater part of the phytoplankton. Of the blue-green algae common in fish ponds, Microcystis aeruginosa is said to be a noxious species. It sometimes forms spectacular water blooms, often with harmful consequences such as depletion of oxygen, poor growth of fish and even mass mortality among the fish. The present study was aimed at investigating the influence of different levels of M. aeruginosa on the water quality and fish production of carp culture ponds. For the present study, three carp culture ponds with high, moderate and low levels of M. aeruginosa were selected. In the three ponds, physico-chemical parameters of water, phyto- and zooplankton and fish production were studied. The results indicated that the fish yield was low with concomitant fish mortalities in the pond with high levels of M. aeruginosa compared to the other two ponds. The influence of the different levels of M. aeruginosa on other planktonic groups and in turn their effect on fish production were analyzed and discussed in the light of the existing literature. Key words: Cyanobacteria, algal blooms, Microcystis, phytoplankton, zooplankton, fish production, carp culture ponds. -
By Matthew George Heffel B.S., Kansas State University, 2019 A
Pandorina morum genome assembly, annotation, and analysis by Matthew George Heffel B.S., Kansas State University, 2019 A THESIS submitted in partial fulfillment of the requirements for the degree MASTER OF SCIENCE Department of Biology College of Arts and Sciences KANSAS STATE UNIVERSITY Manhattan, Kansas 2020 Approved by: Major Professor Bradly J. S. C. Olson Copyright © Matthew G. Heffel 2020. Abstract The evolution of multicellularity is a major evolutionary transition that leads to increased organismal complexity and has occurred various times in multiple domains of life. Despite its common occurrence, the evolution of multicellularity is not yet well understood largely due to genetic signatures being lost due to deep divergence between unicellular and multicellular lineages. The volvocine algae have recently made the transition to multicellularity (200 MYA) and cover a large range of morphologies, including unicellular Chlamydomonas, undifferentiated multicellular Gonium (8-16 cells), multicellular isogamous Pandorina (8-16 cells), multicellular isogamous Yamagishiella (32 cells), multicellular anisogamous Eudorina (32 cells), and multicellular differentiated Volvox with germ-soma division of labor (>500 cells). Using modern sequencing techniques, here, the genome of Pandorina morum is sequenced, assembled, and annotated. Brief comparative genomics work shows gene orthology to related volvocine species as well as a common trend of progressive gene loss occurring at a higher rate than gene gain and organismal complexity increases. -
Freshwater Algae in Britain and Ireland - Bibliography
Freshwater algae in Britain and Ireland - Bibliography Floras, monographs, articles with records and environmental information, together with papers dealing with taxonomic/nomenclatural changes since 2003 (previous update of ‘Coded List’) as well as those helpful for identification purposes. Theses are listed only where available online and include unpublished information. Useful websites are listed at the end of the bibliography. Further links to relevant information (catalogues, websites, photocatalogues) can be found on the site managed by the British Phycological Society (http://www.brphycsoc.org/links.lasso). Abbas A, Godward MBE (1964) Cytology in relation to taxonomy in Chaetophorales. Journal of the Linnean Society, Botany 58: 499–597. Abbott J, Emsley F, Hick T, Stubbins J, Turner WB, West W (1886) Contributions to a fauna and flora of West Yorkshire: algae (exclusive of Diatomaceae). Transactions of the Leeds Naturalists' Club and Scientific Association 1: 69–78, pl.1. Acton E (1909) Coccomyxa subellipsoidea, a new member of the Palmellaceae. Annals of Botany 23: 537–573. Acton E (1916a) On the structure and origin of Cladophora-balls. New Phytologist 15: 1–10. Acton E (1916b) On a new penetrating alga. New Phytologist 15: 97–102. Acton E (1916c) Studies on the nuclear division in desmids. 1. Hyalotheca dissiliens (Smith) Bréb. Annals of Botany 30: 379–382. Adams J (1908) A synopsis of Irish algae, freshwater and marine. Proceedings of the Royal Irish Academy 27B: 11–60. Ahmadjian V (1967) A guide to the algae occurring as lichen symbionts: isolation, culture, cultural physiology and identification. Phycologia 6: 127–166 Allanson BR (1973) The fine structure of the periphyton of Chara sp. -
Protists and Other Organisms on a Minute Snail Periostracum A
Brazilian Journal of Biology https://doi.org/10.1590/1519-6984.186837 ISSN 1519-6984 (Print) Original Article ISSN 1678-4375 (Online) Protists and other organisms on a minute snail periostracum A. López de la Fuentea*, R. J. Urcuyoa and G. H. Vegab aCentro de Malacología, Universidad Centroamericana – UCA, Rotonda Rubén Darío 150mt Oeste, Managua, Nicaragua bEstación Biológica Juan Roberto Zarruk, Universidad Centroamericana – UCA, Rotonda Rubén Darío 150 m Oeste, Managua, Nicaragua *e-mail: [email protected] Received: October 19, 2017 – Accepted: December 21, 2017 – Distributed: August 31, 2019 (With 11 figures) Abstract Since the foundation of the Malacological Center in 1980, Universidad Centro Americana (UCA), Managua-Nicaragua, has been monitoring and collecting the marine, terrestrial, fluvial and lake mollusk population of the country. Many specimens have been photographed by Scanning Electronic Microscope (SEM), and in one of these, observation of the hairy periostracum reveals a seemingly thriving population of minute protists in possible symbiosis with their host. Adequate magnification and comparison with previous studies allowed the determination of these hosts as diatoms, testaceous amoebae, yeast, phacus, spores and other undetermined organisms which occur in tropical forests on rocks, trees and leaves. Here illustrated are diatoms and other organisms detected for the first time on the periostracum of a tropical rainforest mollusk. Keywords: Nicaragua rainforest, minute snail, SEM electrograms, diatoms, protists. Protistas e outros organismos em um pequeno periostracum de caracol Resumo Desde a fundação do Centro Malacológico em 1980, a Universidad Central Americana (UCA), Manágua-Nicarágua, vem acompanhando e coletando a população de moluscos marinhos, terrestres, fluviais e lagoas do país.