DOCSLIB.ORG

The Effects of Isolated Flavonoids From

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Effects of Isolated Flavonoids From THE EFFECTS OF ISOLATED FLAVONOIDS FROM THE TUBEROUS ROOTS OF RED KWAO KREU (Butea superba Roxb.) ON REPRODUCTIVE SYSTEM OF MALE MICE Wanatkamon Naknarong A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science in Environmental Biology Suranaree University of Technology Academic Year 2013 ผลของฟลาโวนอยด์ที่สกัดจากรากกวาวเครือแดง (Butea superba Roxb.) ต่อระบบสืบพนั ธ์ุหนูไมซ์เพศผู้ นางสาววณัทกมล นาคณรงค์ วทิ ยานิพนธ์นีเ้ ป็นส่วนหนึ่งของการศึกษาตามหลกั สูตรปริญญาวทิ ยาศาสตรมหาบัณฑิต สาขาวิชาชีววิทยาสิ่งแวดล้อม มหาวทิ ยาลยั เทคโนโลยสี ุรนารี ปีการศึกษา 2556 I วณัทกมล นาคณรงค์ : ผลของฟลาโวนอยด์ที่สกดั จากรากกวาวเครือแดงตอ่ ระบบสืบพนั ธุ์ หนูไมซ์เพศผู้ (THE EFFECTS OF ISOLATED FLAVONOIDS FROM THE TUBEROUS ROOTS OF RED KWAO KREU (Butea superba Roxb.) ON REPRODUCTIVE SYSTEM OF MALE MICE) อาจารย์ที่ปรึกษา : ผชู้ ่วยศาสตราจารย์ ดร.เกรียงศกั ด์ิ เอ้ือมเกบ็ , 105 หน้า. กวาวเครือแดงไดร้ ับการอา้ งวา่ สามารถใช้ในผู้ชายไทยเพื่อการกลบั สู่ภาวะหนุ่มในการ เพ่ิม สมรรถภาพทางเพศ ป้ องกนั มะเร็งตอ่ มลูกหมากและตอ่ มลูกหมากโต เพราะมีสารแอนโดรเจนจาก พืช ในความเป็นจริงการหยอ่ นสมรรถภาพทางเพศคือการที่ไมส่ ามารถรักษาการแข็งตัวของอวัยวะ เพศชายในการมีกิจกรรมทางเพศ ที่มีสาเหตุมาจากสภาพร่างกายและจิตใจ หลายประการ ยาในกลุ่ม ยบั ย้งั พีดีอี -5 ซิลเดนาฟิล ได้รับการรับรองสาหรับการรักษา อาการหยอ่ นสมรรถภาพทางเพศ นอกจากน้นั ยงั พบวา่ ยาน้ีสามารถเพ่ิมระดบั ฮอร์โมนเทสโทสเตอโรน และกระตุน้ เลดิกเซลลส์ ่งผล ใหเ้ กิดการกระตุน้ กระบวนการสร้างตวั อสุจิ อยา่ งไรกต็ าม รายงานผลข้างเคียง ที่พบบอ่ ย ไดแ้ ก่ อาการปวดศีรษะ หนา้ แดง อาหารไมย่ อ่ ย คดั จมูก และรบกวนการมองเห็นภาพสี ดงั น้นั จุดประสงค์ ของการศึกษาคร้ังน้ีเพื่อศึกษาผลของสารสกดั จากรากของกวาวเครือแดงเปรียบเทียบกบั ซิลเดนาฟิล ตอ่ ระบบสืบพันธุ์ของหนู ไมซ์เพศผู้ จากน้นั ผงแหง้ ของรากพืชกวาวเครือแดง จึงถูกสกดั ดว้ ยเอทา นอล และถูก แยกสารสกดั ออกจากกนั โดยซิลิกาเจลคอลมั น์ดว้ ยเทคนิค โครมาโทกราฟี และ สารประกอบที่ถูกแยก ถูกพิสูจน์เอกลักษณ์เพื่อให้ทราบชื่อโดยใช้เครื่องมือโครมาโทรกราฟีแบบ เฟสเคลื่อนที่เป็นของเหลวสมรรถนะสูง (เฮทพีแอลซี ) โดยการเปรียบเทียบกบั สารมาตรฐาน ตอ่ จากน้นั ไดน้ า สารที่สกดั ไดเ้ หล่าน้ีมา ป้อนหนูเพศผู้โดยให้ทางปากในขนาดที่ให้สารสกดั หยาบ ซิลเดนาฟิล แฟลกชันบี แฟลกชันซี และแฟลกชันอี ไดแ้ ก่ 1,250 10 40 50 และ 150 มิลลิกรัมตอ่ กิโลกรัมตอ่ น้า หนกั ตวั ตอ่ วนั ตามลาดับ ส่วนประกอบของสารสกดั เหล่าน้ีพบวา่ เป็นเจนิสติน และไบโอชานินเอ ทุกกลุ่มไดร้ ับการทดลองเป็นเวลา 14 วนั ติดตอ่ กนั เลือดและสเปิร์มได้ถูกเกบ็ เพื่อการวเิ คราะห์ก่อนและหลงั การ ทดลองในทุกกลุ่ม นอกจากน้ี เมื่อสิ้นสุดการทดลองได้ทาการ เกบ็ ส่วนตา่ งๆ ของอวัยวะสืบพันธุ์และอวัยวะที่สาคัญ เพื่อการเปรียบเทียบ การเปลี่ยนแปลงน้า หนกั ตัวสัมพัทธ์ได้ถูกนามาวิเคราะห์ด้วย ผลการศึกษาพบวา่ น้า หนกั ตวั สัมพทั ธ์ของหนูในกลุ่มที่ไดร้ ับ สารท้งั หมด ไมแ่ ตกตา่ งจากกลุ่มควบคุมอยา่ งมีนยั สา คญั ในขณะที่น้า หนกั มา้ มของหนูในกลุ่มที่ ได้รับ แฟลกชันบี ซี และอี จะมีน้า หนกั มากกวา่ กลุ่มควบคุม ในทางตรงขา้ มกบั น้า หนกั ขอ ง (p<0.01) อยา่ งไรกต็ าม น้า หนกั อณั ฑะในกลุ่มที่ไดร้ ับสารสกดั หยาบและกลุ่มไดร้ ับ ซิลเดนาฟิลน้นั ตลอดจนน้า หนกั ของเอพิดิไดมิส ของกลุ่มที่ไดร้ ับ แฟลกชันอีและซี มีน้า หนกั มากกวา่ กลุ่มควบคุม อยา่ งมีนยั สา คญั ทางสถิติ (p<0.01) ยง่ิ ไปกวา่ น้นั หนูที่ภายหลงั การทดสอบสารทุกกลุ่ม มีระดบั ของ เทสโทสเตอโรนสูงข้ึนอยา่ งมีนยั สา คญั เมื่อเทียบกบั ก่อนการใหส้ าร ยกเวน้ กลุ่มควบคุม II นอกจากน้นั ระดบั ของเทสโทสเตอโรนในกลุ่มที่ไดร้ ับแฟลกชนั ซีและอี ยงั สูงกวา่ กลุ่มอื่นๆอยา่ งมี นยั สา คญั และยงั พบวา่ กลุ่มที่ไดร้ ับแฟลกชนั ซีมีจา นวนตวั อสุจิสูงที่สุด การคน้ พบน้ีเป็นหลกั ฐานที่ แสดงวา่ เจนิสติน สารที่ยงั ไมท่ ราบชื่อ และไบโอคานินเอ อาจมีบทบาทสา คญั ในการเพ่ิมระดบั เทส โทสเทอโรน จา นวนตวั อสุจิ และเพ่ิมการเคลื่อนไหวของตวั อสุจิ หรือการรักษาภาวะการมีบุตรยาก ในผู้ชาย หลังจากที่ ได้วิจัยระดับของความปลอดภัยแล้ว ผลการวเิ คราะห์เลือดพบวา่ กลุ่มที่ไดร้ ับ แฟลกชนั ซี อีและซิลเดนาฟิล มีระดบั คอเลสเตอรอลสูงกวา่ กลุ่มควบคุมอยา่ งมีนยั สา คญั ยง่ิ ไปกวา่ น้นั ระดบั เฮโมโกลบินในกลุ่มที่ไดร้ ับแฟลกชนั อี สูงกวา่ กลุ่มที่ไดร้ ับสารสกดั หยาบและกลุ่ม ควบคุมอยา่ งมีนยั สา คญั (p<0.05) สาขาวิชา ชีววิทยา ลายมือชื่อนักศึกษา __________________________ ปีการศึกษา 2556 ลายมือชื่ออาจารย์ที่ปรึกษา ____________________ III WANATKAMON NAKNARONG : THE EFFECTS OF ISOLATED FLAVONOIDS FROM THE TUBEROUS ROOTS OF RED KWAO KREU (Butea superba Roxb.) ON REPRODUCTIVE SYSTEM OF MALE MICE. THESIS ADVISOR : ASST. PROF. GRIANGSAK EUMKEB, Ph.D. 105 PP. BUTEA SUPERBA ROXB, TESTOSTERONE, SPERM COUNT, SILDENAFIL, CHOLESTEROL Butea superba Roxb.(BS) has been claimed to use in Thai men for rejuvenation, improve sexual function, prevent prostate cancer and prostatic hyperplasia, because it contains Phytoandrogen. In fact, Erectile dysfunction (ED) is the inability to maintain penile erection for the successful performance of sexual activity which is caused by many physical and psychological factors. The PDE-5 inhibitors, sildenafil, is approved for the treatment of ED. In addition, sildenafil has increased testosterone level and stimulated Leydig cells resulting in an increase of spermatogenesis. However, the most frequent adverse effects reported for sildenafil is headache, flushing, dyspepsia, nasal congestion, and disturbances in color vision. Hence, the objective of this study was to investigate the effects of the tuberous root of Butea superba Roxb extract compared with sildenafil on the reproductive system of male mice. Then, the dried powder of the tuber roots of Butea superba Roxb. was extracted with ethanol and separated by silica gel column chromatography The chemical names of the isolated compounds were identified by High performance Liquid Chromatography (HPLC) compared with standard compounds. Then, these compounds were orally administered to male mice. The crude extract, sildanafil, fraction B, C and D were fed at the doses of 1,250, 10, 40, 50 and 150 mg /kg BW/day respectively. The majority of these extract compounds such as Genistein and IV Biochanin A were elucidated. All groups were treated for 14 consecutive days. Blood and sperms were collected for analysis before (pre-) and after (post-) treatment in all groups. Also, at the end of the experiments, the selected reproductive and vital organs were collected for comparative measurements. A relative change of body weight was also analyzed. The results showed that the relative body weights in all treated groups were not significant different from the control. Whereas, the spleen weight of fraction B, C and E treated groups were significantly heavier, and stomach weight of fraction E group was lighter than those of the control (p<0.01). However, the testes weight of crude extract and sildenafil treated groups, and the epididymis weight of fraction E and C treated group were significantly heavier than those of the control (p<0.01). In addition, the results exhibited that there were significant increases in testosterone levels of all post-treated groups compared to pre-treatment groups, except for that of the control (p<0.01). In addition, the testosterone levels of fraction C and E treated groups were significantly higher than those of other groups (p<0.01). Also, the sperm number of all treated groups were significantly higher than that of the control group (p<0.01) as well as the fraction C which showed the highest number. These findings provide evidence that Genistein, Unknown compound 1 and Biochanin A may play an important role in increasing of testosterone level, sperm number and motility or infertility treatment in men after safety level is investigated. The blood analysis showed that the cholesterol level of fraction C, E and sildenafil were significantly higher than those of the control (p<0.05). Morover, the hemoglobin level of fraction E treated group was significantly higher than those of the control and the crude extract groups (p<0.05). School of Biology Student’s Signature_____________________ Academic Year 2013 Advisor’s Signature_____________________ V ACKNOWLEDGEMENTS I would like to express my appreciation for Master degree Scholarship that Suranaree University of Technology offered in my thesis. I would like to thank my thesis advisor, Asst. Prof. Dr.Griangsak Eumkeb for giving me the opportunity to study on this thesis, and his valuable guidance and suggestions throughout my study. My appreciation also goes to the committee members, Asst. Prof. Dr. Nooduan Muangsan, Assoc. Prof. Dr.Sajeera Kupittayanant and Dr. Pongrit Krubphachaya for their helpful and useful suggestions. I would like to extend my sincere thanks to all staffs and my friends of the Center for Scientific Technology and Equipment at F1, F2 and Animal Care Building at Suranaree University of Technology, particularly in Biology laboratory. It was my great pleasure knowing all of them. I would also like to thank all of my good friends at the School of Biology, Suranaree University of Technology for their friendship and all the help they have provided. Most of all, my deepest gratitude is extended to my parents and cousins for their infinite love, understanding and constant encouragement throughout my study. Wanatkamon Naknarong VI CONTENTS Page ABSTRACT IN THAI ................................................................................................... I ABSTRACT IN ENGLISH ........................................................................................ III ACKNOWLEDGEMENTS .......................................................................................... V CONTENTS ................................................................................................................ VI LIST OF TABLES .....................................................................................................
Load more

Recommended publications
  • 192-208 (2011) Ewsletter Parashar and Dhamija
    Pharmacologyonline 3: 192-208 (2011) ewsletter Parashar and Dhamija BOTAICAL, PHYTOCHEMICAL AD BIOLOGICAL IVESTIGATIO OF BUTEA MOOAPERMA (LAM.) KUTZE Bharat Parashar, Hitesh Kumar Dhamija* Department of Pharmacy Manav Bharti University, Solan, H.P. (India) Corresponding Author: - Hitesh Kumar Dhamija Assistant Professor, Department of Pharmay Manav Bharti University, Village - Laddo, P.O. : - Sultanpur (Kumhar Hatti), Tehsil & Distt. Solan -173229 Himachal Pardesh (India) Contact No. 8894434403 E-Mail:- [email protected] [email protected] Summary In the last few decades there has been an exponential growth in the field of herbal medicine. It is getting popularized in developing and developed countries owing to its natural origin and lesser side effects. One such medicinal plant is Butea monosperma (Lam.) Kuntze ( Fabaceae ), which is commonly used as palas. It is a medium sized deciduous tree which is mainly found in ascending of Himalayas up to 900 m height and in peninsular India up to 1200 m height. All parts of plant are important viz . Bark, leaves, flowers, seed etc. This plant is used ethanobotanically in Ayurveda, Unani, Homeopathic and Allopathic system of medicines as astringent, aphrodisiac, antifertility, analgesic, burning sensation, diuretics, diarrhea, dysentery, depurative, filariasis, gout, helminthiasis, leprosy, night blindness, piles, skin diseases, sore throat, snake bite, tumours, ulcer etc. The plant is reported to possess anthelmintic, anti convulsion, anti diarrheal, anti fungal, anti diabetic, hypoglycemic, anti hyperlipidaemic, anti inflammatory, anti oxidant, wound healing, anti fertility and hepatoprotective Activity. All these uses are due to the present of certain phytoconstituent in the plant. The major phytoconstituent reported in this plant are Isobutrin, butrin, butin, butein, chalcones, aurones, isobutyine, palasitrin, 3',4',7- trihydroxyflavone, palsitrin, Coreopsin, isocoreopsin, sulphurein, monospermoside, isomonospermoside, quercetin, prunetin, Lupenone, lupeol.
    [Show full text]
  • Floristic and Phytoclimatic Study of an Indigenous Small Scale Natural Landscape Vegetation of Jhargram District, West Bengal, India
    DOI: https://doi.org/10.31357/jtfe.v10i1.4686 Sen and Bhakat/ Journal of Tropical Forestry and Environment Vol. 10 No. 01 (2020) 17-39 Floristic and Phytoclimatic Study of an Indigenous Small Scale Natural Landscape Vegetation of Jhargram District, West Bengal, India U.K. Sen* and R.K. Bhakat Ecology and Taxonomy Laboratory, Department of Botany and Forestry, Vidyasagar University, West Bengal, India Date Received: 29-09-2019 Date Accepted: 28-06-2020 Abstract Sacred groves are distinctive examples of biotic components as genetic resources being preserved in situ and serve as secure heavens for many endangered and endemic taxa. From this point of view, the biological spectrum, leaf spectrum and conservation status of the current sacred grove vegetation, SBT (Swarga Bauri Than) in Jhargram district of West Bengal, India, have been studied. The area's floristic study revealed that SBT‟s angiosperms were varied and consisted of 307 species belonging to 249 genera, distributed under 79 families of 36 orders as per APG IV. Fabales (12.05%) and Fabaceae (11.73%) are the dominant order and family in terms of species wealth. Biological spectrum indicates that the region enjoys “thero-chamae-cryptophytic” type of phytoclimate. With respect to the spectrum of the leaf size, mesophyll (14.05%) was found to be high followed by notophyll (7.84%), microphyll (7.19%), macrophyll (7.84%), nanophyll (6.86%), leptophyll (6.21%), and megaphyll (2.29%). The study area, being a sacred grove, it has a comparatively undisturbed status, and the protection of germplasm in the grove is based on traditional belief in the social system.
    [Show full text]
  • Butea Monosperma
    Available online on www.ijpcr.com International Journal of Pharmaceutical and Clinical Research 2010,2(2), 90-94 Review Article ISSN 0975 1556 PLANT REVIEW: Butea monosperma Sindhia V.R.1, Bairwa R.2 1Institute of Pharmacy, Bundelkhand University Jhansi (U.P.),India 2School of Pharmaceutical Sciences, Jaipur National University, Jaipur, India ABSTRACT Butea monosperma (Lam.) Kuntze (Syn. Butea frondosa; Family Fabaceae) , This is a moderate sized deciduous tree which is widely distributed throughout India , Burma and Ceylon, popularly known as 'dhak' or 'palas', commonly known as ‘Flame of forest’. The family Fabaceae compromises of 630 genera and 18,000 species. The crude extracts of various parts and pure isolates of Butea monosperma was reported to possess antibacterial, antifungal, hypoglycemic, anti-inflammatory activities. Butea monosperma have been found to possess tonic, astringent, aphrodisiac and diuretics properties. The widespread uses of Butea monosperma in traditional systems of medicine have resulted in their extensive chemical analysis for their bio-active principles. This article briefly reviews the botany, chemistry and pharmacology of Butea monosperma (Lam) Kuntze. Keywords: Butea monosperma, fabacear, dhak, palas. INTRODUCTION It is one of the most beautiful tree has been put to some It is evident that without nature human being life is useful purpose. Butea monosperma is extensibly used in impossible. There are three basic necessity of humans is Ayurveda, Unani and Homeopathic medicine and has food, clothes and shelter and now the fourth one is good become a cynosure of modern medicine. The plants of health, which provided by plant kingdom. Nature stands this genus are well known for their colouring matters.
    [Show full text]
  • Flemingia </I> Subgenus <I> Rhynchosioides</I>
    Blumea 64, 2019: 253–271 www.ingentaconnect.com/content/nhn/blumea RESEARCH ARTICLE https://doi.org/10.3767/blumea.2019.64.03.06 Taxonomic revision and molecular phylogeny of Flemingia subgenus Rhynchosioides (Leguminosae) S.K. Gavade1†, S. Surveswaran2†, L.J.G. van der Maesen3, M.M. Lekhak1* Key words Abstract A taxonomic revision of Flemingia subg. Rhynchosioides based on morphology and molecular information (matK and ITS) is presented. The subgenus comprises six herbaceous taxa (F. gracilis, F. mukerjeeana, F. nilgherien­ Cajaninae sis, F. rollae, F. tuberosa and F. vestita). All species except F. vestita are endemic to India. Morphological evidence endemism and molecular phylogeny revealed that the subgenus is monophyletic. Nevertheless, the systematic position of lateritic plateaus F. tuberosa remains unclear on account of its unique ecology and inflorescence. A new species, F. mukerjeeana, is molecular phylogeny described and four binomials, namely F. gracilis, F. nilgheriensis, F. tuberosa and F. vestita have been lectotypified. taxonomy Furthermore, all species have been described, illustrated and their ecology discussed. A taxonomic key including tuber crops the recently described species from Thailand, F. sirindhorniae, is also provided for easy identification. Published on 31 October 2019 INTRODUCTION scription by mentioning that the flowers are in corymbs or in long-peduncled heads. Baker (1876) included three taxa, Flemingia Roxb. ex W.T.Aiton (Leguminosae Juss., Papilio­ namely F. tuberosa Dalzell, F. vestita and F. vestita var. nilghe­ noideae DC.) is an old world genus. It is one of the members of riensis Baker (now F. nilgheriensis (Baker) Wight ex Cooke). the quaternary gene pool of Cajanus cajan (L.) Millsp.
    [Show full text]
  • Butea Superba Roxb
    ISSN 0100-879X Volume 43 (9) 812-913 September 2010 BIOMEDICAL SCIENCES AND www.bjournal.com.br CLINICAL INVESTIGATION Braz J Med Biol Res, September 2010, Volume 43(9) 843-852 doi: 10.1590/S0100-879X2010007500076 Luteinizing hormone reduction by the male potency herb, Butea superba Roxb. S. Malaivijitnond, A. Ketsuwan, G. Watanabe, K. Taya and W. Cherdshewasart The Brazilian Journal of Medical and Biological Research is partially financed by Institutional Sponsors Hotsite of proteomics metabolomics developped by: Campus Ribeirão Preto Faculdade de Medicina de Ribeirão Preto analiticaweb.com.br S C I E N T I F I C Brazilian Journal of Medical and Biological Research (2010) 43: 843-852 ISSN 0100-879X Luteinizing hormone reduction by the male potency herb, Butea superba Roxb. S. Malaivijitnond1, A. Ketsuwan1,2, G. Watanabe3,4, K. Taya3,4 and W. Cherdshewasart1 1Primate Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 2Interdisciplinary Program in Physiology, Graduate School, Chulalongkorn University, Bangkok, Thailand 3Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo, Japan 4Department of Basic Veterinary Science, the United Graduate School of Veterinary Science, Gifu University, Gifu, Japan Abstract To determine if Butea superba Roxb., a traditional Thai male potency herb, has androgenic activity in 60-day-old male Wistar rats, we measured its effects on the pituitary-testicular axis and sex organs. Intact and orchidectomized adult male rats were subdivided into five groups (10 rats/group): distilled water, Butea superba (BS)-10, BS-50, BS-250, and testosterone propi- onate (TP). They received 0, 10, 50, and 250 mg·kg body weight-1·day-1 BS in distilled water by gavage and 6 mg·kg body weight-1·day-1 TP sc, respectively, during the 30-day treatment period.
    [Show full text]
  • 22 a Valuable Insight Into the Phytochemistry, Pharmacognosy
    International Journal of Pharmaceutical Science and Research International Journal of Pharmaceutical Science and Research ISSN: 2455-4685, Impact Factor: RJIF 5.28 www.pharmacyjournal.net Volume 1; Issue 6; September 2016; Page No. 22-32 A valuable insight into the phytochemistry, Pharmacognosy and pharmacology of Palash Anshul Chawla CT Institute of Pharmaceutical Sciences, Jalandhar, Punjab, India Abstract India has one of the largest medicinal garden consists of millions of plants and their species. Nowadays, applications of herbal medicines and their scope is far most important for researchers. In leu of this the present discussion is based on one of the most precious plant named as Palash. Here some pharmacognostics and phytochemical characteristics of the plant are discussed with their appropriate references. This study also opens the door for further research in this direction which would be of our kind interest. Keywords: phytochemistry, Pharmacognosy, pharmacology, Palash 1. Introduction Butea monosperma is a moderate sized deciduous tree which is 1.2 Local names widely distributed throughout India, Burma and Ceylon, Bengali- palas, kinaka, peras, polashi; English- flame-of-the- popularly known as 'palash', commonly known as ‘Flame of forest, bastard teak; Hindi- chichratesu, polak, tellamoduga, forest’. The family Fabaceae compromises of 630 genera and dhak, palas, desukajhad, khankrei, chalcha; Urdu- palash,papra; 18,000 species. Different Species Available of Genus Butea: Sanskrit- brahmopadapa, lakshataru, palasha [42] Butte acuminata, Butea affinis, Butea Africana, Butea apoensis, Butea balansae, Butea braamiana, Butea bracteolate, Butea 1.3 Biological source cuneiforms, Butea crass folia, Butea dubia, Butea ferruginous, It is obtained from whole tree of Butea monosperma.
    [Show full text]
  • Qualitative Analysis of Inorganic Acid, Basic Radicals, and Estimation of Biomolecules in the Flower Extract of Butea Superba Roxb
    Online - 2455-3891 Vol 11, Issue 10, 2018 Print - 0974-2441 Research Article QUALITATIVE ANALYSIS OF INORGANIC ACID, BASIC RADICALS, AND ESTIMATION OF BIOMOLECULES IN THE FLOWER EXTRACT OF BUTEA SUPERBA ROXB. SEETHALAKSHMI B1*, KAVITHA K2 1 Assistant Professor, Department of Humanities and Science, Mugavari Eye Hospital and Research Institute, Medavakkam, Chennai - 600 100, Tamil Nadu, India. 2Research Scholar, Department of Zoology, Guru Nanak College, Velachery, Chennai - 600 042, Tamil Nadu, India. Email: [email protected] Received: 03 May 2018, Revised and Accepted: 12 June 2018 ABSTRACT Objective: Medicinal plants have different chemicals in their roots, stem, leaves, flowers, and fruits. In human history, medicinal plants play a vital role in the traditional medicine because of herbal plants have a great nutritive value, and some of them are also used in medicine. The efficacy of medicinal plants is mainly allied with their components such as secondary metabolites and inorganic acid, and basic radicals considered that prolonged intake can cause health problems if the heavy metals such as mercury, cadmium, and other impurities are the threshold concentrations. Methods: In the present study, the flower extract of Butea superba was analyzed for the inorganic acid and basic radicals and the estimation of total saponins, terpenoids, and alkaloids. The above protocols were done using standard methods. Results: The flower extract of B. superba revealed that the inorganic acid radicals such as chromate, phosphate, and sulfate and the basic radicals such as lead, arsenic, and ferrous showed that positive results. The percentage of total saponins, terpenoids, and alkaloids were compared in the following order, saponins<terpenoids<alkaloids as 4.181±0.017%<79.833±0.351%<107.823±0.105%, respectively.
    [Show full text]
  • Butea Monosperma
    Rai Geeta et al. IRJP 2 (7) 2011 98-108 INTERNATIONAL RESEARCH JOURNAL OF PHARMACY ISSN 2230 – 8407 Available online http://www.irjponline.com Review Article BUTEA MONOSPERMA (LAM.) KUNTZE: A REVIEW Rai Geeta*1, Rajak Prakash2, Sandhu Navgeet3, Vasudeva Neeru4, Jindal Sumit3 1United Institute of Pharmacy, Naini, Allahabad-211010, U.P., India 2Institute of Pharmacy and Emerging Sciences, Baddi University of Emerging Sciences and Technology, Makhnumajra, Baddi-173 205, H.P., India 3Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra – 13611, Haryana, India 4Department of Pharmaceutical Sciences, GJUS&T, Hissar-125001, Haryana, India Article Received on: 21/04/11 Revised on: 29/05/11 Approved for publication: 20/06/11 *Geeta Rai, United Institute of Pharmacy, Naini, Allahabad-211010, U.P., India ABSTRACT The traditional systems of medicine together with homoeopathy and folklore medicine continue to play a significant role largely in the health care system of the population. Butea monosperma (Palas) belonging to the family leguminaceae grown wildly in many parts of India. The plant is highly used by the rural and tribal people in curing various disorders. The present paper enumerates various traditional and medicinal utility of the plant and attempt was made to gather information about the chemical composition and pharmacological aspects of the plant. KEYWORDS: Butea monosperma, Palas, Phytochemistry, Medicinal uses INTRODUCTION on the wood, and forming very large racemose panicles Butea monosperma (Palas) is a medium-sized deciduous and two very small ones immediately beneath the tree belongs to family Leguminosae- Papilioneae. This flowers. Fruits are pods with a single seed in each. Pods tree is also called ‘Flame of the Forest’ and Bastard ripen in May-June.
    [Show full text]
  • Cambodian Journal of Natural History
    Cambodian Journal of Natural History New orchid records Ethnobotanical knowledge Carbon stocks and dynamics A homage to Pauline Dy Phon National Biodiversity Action Plan Movement of Siamese crocodiles Payments for Ecosystem Services Camera trapping of large mammals June 2017 Vol. 2017 No. 1 Cambodian Journal of Natural History Editors Email: [email protected] • Dr Neil M. Furey, Chief Editor, Fauna & Flora International, Cambodia. • Dr Jenny C. Daltry, Senior Conservation Biologist, Fauna & Flora International, UK. • Dr Nicholas J. Souter, Mekong Case Study Manager, Conservation International, Cambodia. • Dr Ith Saveng, Project Manager, University Capacity Building Project, Fauna & Flora International, Cambodia. International Editorial Board • Dr Stephen J. Browne, Fauna & Flora International, • Dr Sovanmoly Hul, Muséum National d’Histoire U.K. Naturelle, France. • Dr Martin Fisher, Editor of Oryx – The International • Dr Andy L. Maxwell, World Wide Fund for Nature, Journal of Conservation, U.K. Cambodia. • Dr L. Lee Grismer, La Sierra University, California, • Dr Brad Pett itt , Murdoch University, Australia. USA. • Dr Campbell O. Webb, Harvard University Herbaria, • Dr Knud E. Heller, Nykøbing Falster Zoo, Denmark. USA. Other peer reviewers • Prof. Henrik Balslev, Aarhus University, Denmark. • Dr Le Phat Quoi, Institute for Environment and Natural Resources, Ho Chi Minh National University, Vietnam. • Dr Chou Ly, Virginia Tech, USA. • Dr Benjamin Rawson, World Wide Fund For Nature, • Dr J.W. Duckworth, IUCN SSC Asian Species Action Vietnam. Partnership, UK. • Dr Sasaki Nophea, Asian Institute of Technology, • Jonathan Eames, BirdLife International Cambodia Thailand. Programme. • Dr André Schuiteman, Royal Botanic Gardens, Kew, • Dr Tracy Farrell, Conservation International, Cambodia. UK. • Paul Herbertson, Fauna & Flora International, UK.
    [Show full text]
  • Çòçà¤Ã×Íá´§ (Butea Superba Roxb
    การศึกษาเปรียบเทียบผลของกวาวเครือแดง (Butea superba Roxb.) ที่พบในพื้นที่ที่แตก ตางกันสองพื้นที่ตออวัยวะสืบพันธุ พฤติกรรมการสืบพันธุ และการแข็งตัว ของอวัยวะเพศในหนูขาวเพศผู (Rattus norvegicus) นาย สิทธิศักดิ์ ปนมงคลกุล วิทยานิพนธนี้เปนสวนหนึ่งของการศึกษาตามหลักสูตรปริญญาวิทยาศาสตรมหาบัณฑิต สาขาวิชา ชีววิทยาสิ่งแวดลอม มหาวิทยาลัยเทคโนโลยีสุรนารี ปการศึกษา 2544 ISBN 974 – 533 – 022 - 1 COMPARISON OF THE EFFECTS OF RED KWAO KREUR (BUTEA SUPERBA ROXB.) FROM TWO DIFFERENT AREAS ON REPRODUCTIVE ORGANS, REPRODUCTIVE BEHAVIOR AND ERECTION IN MALE ALBINO RAT (RATTUS NORVEGICUS) Mr. Sitthisak Pinmongkholgul A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science in Environmental Biology Suranaree University of Technology Academic Year 2001 ISBN 974 – 533 – 022 - 1 การศึกษาเปรียบเทียบผลของกวาวเครือแดง (Butea superba Roxb.) ที่พบในพื้นที่ที่แตกตางกันสอง พื้นที่ตออวัยวะสืบพันธุ พฤติกรรมการสืบพันธุ และการแข็งตัว ของอวัยวะเพศในหนูขาวเพศผู (Rattus norvegicus) สภามหาวิทยาลัย มหาวิทยาลัยเทคโนโลยีสุรนารี อนุมัติใหนับวิทยานิพนธฉบับนี้เปน สวนหนึ่งของการศึกษาตามหลักสูตรปริญญามหาบัณฑิต คณะกรรมการสอบวิทยานิพนธ ……………………………………….. ( รศ.ดร.สมพงษ ธรรมถาวร ) ประธานกรรมการ ……………………………………….. ( ดร. วารี วิดจายา ) อาจารยที่ปรึกษา ……………………………………….. ( รศ. ยุทธนา สมิตะสิริ ) กรรมการ ……………………………………….. ( รศ.น.สพ.ดร. เทอด เทศประทีป ) กรรมการ ………………………………………… …………………………………….…. ( รศ.ดร. ทวิช จิตรสมบูรณ ) ( รศ.ดร.ประสาท สืบคา ) รองอธิการบดีฝายวิชาการ คณบดีสํ านักวิชาวิทยาศาสตร สิทธิศักดิ์ ปนมงคลกุล : การศึกษาเปรียบเทียบผลของกวาวเครือแดง
    [Show full text]
  • PSYCHOACTIVE SUBSTANCES a Guide to Ethnobotanical Plants and Herbs, Synthetic Chemicals, Compounds and Products
    PSYCHOACTIVE SUBSTANCES A Guide to Ethnobotanical Plants and Herbs, Synthetic Chemicals, Compounds and Products. Acknowledgements: Acknowledgement: This Guide was compiled by Sinead O’Mahony Carey, Drug Education Officer, Substance Misuse Service South Tipperary. The Substance Misuse Service would like to thank Aine de Leastar, Anne-Marie Dunne and Karen O’Loughlin for their contribution and endless hours of research and referencing. Health Service Executive South Psychoactive Substances Manual (Edition 1.1) Compiled by: Sinead O’Mahony Carey Drug Education Officer Substance Misuse Service 11-12 Peter St., Clonmel. (052) 6177900 Graphic Design: Leo McCormack (Swift Design - 087 6499990) Introduction The ‘Guide to Ethnobotanical Plants and Herbs, Synthetic Chemicals, Compounds and Products’ available to the Irish Market has been collated and designed by the Drug Education Officer and team in the Substance Misuse Service HSE, South Tipperary. This edition is a follow on to ‘A Guide to Substance Misuse for Medical Professionals’ launched in November 2009. This Guide has been produced in response to requests from workers, users, medical professionals and academics for information on the emerging issue of legal herbal highs, plants and chemical alternatives available to buy in shops and online. Legal highs, legal alternatives and natural highs have become a world wide phenomenon in recent years both here in Ireland and on the International stage. The current concern for users, parents and professionals working with people using and misusing Psychoactive Substances is their after effects and how they can negatively impact on the body and mind. A Psychoactive Substance is a drug (any substance that changes the way a body and mind feels acts or thinks) that is not controlled under the Misuse of Drugs Act 1977-84 and is therefore legal to use and possess.
    [Show full text]
  • Research Journal of Pharmaceutical, Biological and Chemical Sciences
    ISSN: 0975-8585 Research Journal of Pharmaceutical, Biological and Chemical Sciences A Plant Review: Butea Monosperma (Lam.) Kuntze Ashish Mishra*, Saket Verma, , Abhinav Prasoon Mishra Advance Institute of Biotech and Paramedical Sciences, Kanpur, India. ABSTRACT Butea monosperma (Lam.) Kuntze (Syn. Butea frondosa; Family Fabaceae), This is a moderate sized deciduous tree which is widely distributed throughout India, Burma and Ceylon, popularly known as 'dhak' or 'palas', commonly known as ‘Flame of forest’. The family Fabaceae compromises of 630 genera and 18,000 species. It finds use both medicinally and commercially with each part of the plant having utility. This plant species has been found to display a wide variety of biological activities. The plant is traditionally reported to possess astringent, bitter, alterative, aphrodiasiac, anthelmintic, antibacterial and anti-asthamatic properties. Bark yields red juice known as ‘Butea gum’or‘Bengalkino’. Previous phytochemical examination of this plant indicated the presence of various constituents some of these are Cajanin and isoformononetin; Stigmasterol; Butin; two known flavonoids, isobutrin (3, 4, 2', 4'-tetrahydroxychalcone-3, 4'- diglucoside), and the less active butrin (7, 3', 4'- trihydroxyflavanone-7, 3'-diglucoside); free sugars and free amino acids and (-)-3-hydroxy-9- methoxypterocarpan (medicarpin) which were isolated from stem-bark extract; bark; seeds; flower; the petroleum ether extract of flowers and petroleum and ethyl acetate extract of stem bark. Its reported pharmacological properties include anthelmintic, anticonceptive, anticonvulsive, antidiabetic, antidiarrhoeal, antiestrogenic and antifertility, antiinflammatory, antimicrobial, antifungal, antibacterial, antistress, chemopreventive, haemaggultinating, hepatoprotective, radical scavenging, Thyroid inhibitory, antiperoxidative and hypoglycemic effects and wound healing activities. It is powerful astringent and is given in many forms of chronic diarrhoea.
    [Show full text]