Anti-NME2 (Aa 1-100) Polyclonal Antibody (DPABH- 15730) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

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Anti-NME2 (aa 1-100) polyclonal antibody (DPABH- 15730) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Antigen Description Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Specificity The peptide sequence for this antibody is 100% homologous with human NME1-NME2 (accession # Q32Q12). However, it has not been tested experimentally for reactivity to NME1. Immunogen Synthetic peptide conjugated to KLH, from within N terminal amino acids 1-100 of Human NME2. Please note that the immunogen used is 100% conserved in NME1 and it is likely that this antibody will alos recognise NME1 (not tested). Isotype IgG Source/Host Rabbit Species Reactivity Mouse, Human Purification Protein G purified Conjugate Unconjugated Applications ELISA, WB, IHC-P Format Liquid Size 100 μg Buffer Constituents: PBS Preservative 0.09% Sodium Azide Storage Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. GENE INFORMATION Gene Name NME2 non-metastatic cells 2, protein (NM23B) expressed in [ Homo sapiens ] 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Official Symbol NME2 Synonyms NME2; non-metastatic cells 2, protein (NM23B) expressed in; nucleoside diphosphate kinase B; NM23 H2; NDP kinase B; c-myc transcription factor; histidine protein kinase NDKB; nucleotide diphosphate kinase B; c-myc purine-binding transcription factor PUF; non-metastatic cells 2, protein (NM23) expressed in; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2; MGC2212; MGC111212; Entrez Gene ID 4831 Protein Refseq NP_001018147 UniProt ID P22392 Chromosome Location 17q21.33 Pathway Metabolic pathways; Metabolism; Metabolism of nucleotides; Nucleotide Metabolism; Purine metabolism; Pyrimidine metabolism; Function ATP binding; DNA binding; kinase activity; metal ion binding; nucleoside diphosphate kinase activity; nucleotide binding; protein binding 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.

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Datasheet: VMA00345KT Product Details
Datasheet: VMA00345KT Description: NUCLEOSIDE DIPHOSPHATE KINASE A ANTIBODY WITH CONTROL LYSATE Specificity: NUCLEOSIDE DIPHOSPHATE KINASE A Format: Purified Product Type: PrecisionAb™ Monoclonal Isotype: IgG2b Quantity: 2 Westerns Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Western Blotting 1/1000 PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type. Target Species Human Species Cross Reacts with: Mouse, Rat Reactivity N.B. Antibody reactivity and working conditions may vary between species. Product Form Purified IgG - liquid Preparation 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G Buffer Solution Phosphate buffered saline Preservative 0.09% Sodium Azide (NaN3) Stabilisers Immunogen Recombinant human nucleoside diphosphate kinase A External Database Links UniProt: P15531 Related reagents Entrez Gene: 4830 NME1 Related reagents Synonyms NDPKA, NM23 Page 1 of 3 Specificity Mouse anti Human nucleoside diphosphate kinase A antibody recognizes nucleoside diphosphate kinase A, also known as NDP kinase A, granzyme A-activated DNase, metastasis inhibition factor nm23, non-metastatic cells 1 and tumor metastatic process-associated protein. The NME1 gene was identified because of its reduced mRNA transcript levels in highly metastatic cells.
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Laboratory Investigation (2018) 98, 164–174 © 2018 USCAP, Inc All rights reserved 0023-6837/18 EDITORIAL The NDPK/NME superfamily: state of the art Mathieu Boissan1,2, Uwe Schlattner3 and Marie-Lise Lacombe1 Nucleoside diphosphate kinases (NDPK) are nucleotide metabolism enzymes encoded by NME genes (also called NM23). Given the fact that not all NME-encoded proteins are catalytically active NDPKs and that NM23 generally refers to clinical studies on metastasis, we use here NME/NDPK to denote the proteins. Since their discovery in the 1950’s, NMEs/NDPKs have been shown to be involved in multiple physiological and pathological cellular processes, but the molecular mechanisms have not been fully determined. Recent progress in elucidating these underlying mechanisms has been presented by experts in the field at the 10th International Congress on the NDPK/NME/AWD protein family in October 2016 in Dubrovnik, Croatia, and is summarized in review articles or original research in this and an upcoming issue of Laboratory Investigation. Within this editorial, we discuss three major cellular processes that involve members of the multi-functional NME/NDPK family: (i) cancer and metastasis dissemination, (ii) membrane remodeling and nucleotide channeling, and iii) protein histidine phosphorylation. Laboratory Investigation (2018) 98, 164–174; doi:10.1038/labinvest.2017.137 ucleoside diphosphate kinases mitochondria (Lacombe et al, upcoming issue8). (NDPK) are nucleotide metabolism NME1, NME2, NME3, and NME4 form hexamers enzymes encoded by NME (also as the catalytically active form (Ćetković et al, next named NM23) genes. NDPKs are issue9). Group II is comprised of more divergent ubiquitous enzymes catalyzing the proteins sharing only 22 to 44% identity with Ntransfer of a phosphate from nucleoside group I enzymes and between each other.
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Molecular Mechanisms Underlying the Metastasis Suppressor Activity of NME1 in Malignant Melanoma Item Type dissertation Authors Pamidimukkala, Nidhi Vela Publication Date 2019 Abstract Metastatic melanoma is exceedingly lethal and is responsible for the majority of skin cancer related deaths. Impactful research on melanoma metastasis is crucial to improving prognosis. The discovery of metastasis suppressor genes, genes that inhibit... Keywords ALDOC; NME1; Metastasis; Fructose-Bisphosphate Aldolase; Melanoma; Transcription, Genetic Download date 26/09/2021 16:56:05 Link to Item http://hdl.handle.net/10713/9611 Curriculum Vitae Nidhi V. Pamidimukkala [email protected] https://www.linkedin.com/in/nidhi-pamidimukkala Doctor of Philosophy, 2019 EDUCATION 2012-2019 Ph.D., Graduate Program in Molecular Medicine, Cancer Biology Tack University of Maryland School of Medicine – Baltimore, MD 2005-2009 B.S., Biological Sciences, Mellon College of Science Carnegie Mellon University - Pittsburgh, PA 2005-2009 Minor, Chemistry, Mellon College of Science Carnegie Mellon University - Pittsburgh, PA RESEARCH EXPERIENCE 2012-2019 Graduate Research Assistant, Mentor: David M. Kaetzel, Ph.D University of Maryland School of Medicine-Baltimore, MD 2009-2012 Biochemist-MBV Bioprocessing Group Merck & Co, Inc.-Union, NJ 2008-2008 Future Talent Intern-Drug Metabolism Group Merck & Co., Inc- Rahway, NJ 2007-2009 Undergraduate Research Assistant, Mentor: Gordon S. Rule, Ph.D Carnegie Mellon University-Pittsburgh, PA RESEARCH PUBLICATIONS Pamidimukkala, N.V , Leonard, M.K, Snyder, D., McCorkle, J.R., Kaetzel, D.M. (2018). Metastasis Suppressor NME1 Directly Activates Transcription of the ALDOC Gene in Melanoma Cells. Anticancer Research,38(11), 6059-6068. PMID: 30396920 Leonard, M.K, Novak, M., Snyder, D., Snow, G., Pamidimukkala, N.V , McCorkle, J.R., Yang, X.H., Kaetzel, D.M.
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