NME2 (NM 001018139) Human Recombinant Protein Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TP300680 NME2 (NM_001018139) Human Recombinant Protein Product data: Product Type: Recombinant Proteins Description: Recombinant protein of human non-metastatic cells 2, protein (NM23B) expressed in (NME2), transcript variant 4 Species: Human Expression Host: HEK293T Tag: C-Myc/DDK Predicted MW: 17.1 kDa Concentration: >50 ug/mL as determined by microplate BCA method Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Storage: Store at -80°C. Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. RefSeq: NP_001018149 Locus ID: 4831 UniProt ID: P22392, Q6FHN3 RefSeq Size: 682 Cytogenetics: 17q21.33 RefSeq ORF: 456 Synonyms: NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 NME2 (NM_001018139) Human Recombinant Protein – TP300680 Summary: Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010] Protein Families: Druggable Genome, Transcription Factors Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism Product images: Coomassie blue staining of purified NME2 protein (Cat# TP300680). The protein was produced from HEK293T cells transfected with NME2 cDNA clone (Cat# [RC200680]) using MegaTran 2.0 (Cat# [TT210002]). This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 2.

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Datasheet: VMA00345KT Product Details
Datasheet: VMA00345KT Description: NUCLEOSIDE DIPHOSPHATE KINASE A ANTIBODY WITH CONTROL LYSATE Specificity: NUCLEOSIDE DIPHOSPHATE KINASE A Format: Purified Product Type: PrecisionAb™ Monoclonal Isotype: IgG2b Quantity: 2 Westerns Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Western Blotting 1/1000 PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type. Target Species Human Species Cross Reacts with: Mouse, Rat Reactivity N.B. Antibody reactivity and working conditions may vary between species. Product Form Purified IgG - liquid Preparation 20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G Buffer Solution Phosphate buffered saline Preservative 0.09% Sodium Azide (NaN3) Stabilisers Immunogen Recombinant human nucleoside diphosphate kinase A External Database Links UniProt: P15531 Related reagents Entrez Gene: 4830 NME1 Related reagents Synonyms NDPKA, NM23 Page 1 of 3 Specificity Mouse anti Human nucleoside diphosphate kinase A antibody recognizes nucleoside diphosphate kinase A, also known as NDP kinase A, granzyme A-activated DNase, metastasis inhibition factor nm23, non-metastatic cells 1 and tumor metastatic process-associated protein. The NME1 gene was identified because of its reduced mRNA transcript levels in highly metastatic cells.
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Identi Cation of Crucial Genes in Metastatic Osteosarcoma And
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Laboratory Investigation (2018) 98, 164–174 © 2018 USCAP, Inc All rights reserved 0023-6837/18 EDITORIAL The NDPK/NME superfamily: state of the art Mathieu Boissan1,2, Uwe Schlattner3 and Marie-Lise Lacombe1 Nucleoside diphosphate kinases (NDPK) are nucleotide metabolism enzymes encoded by NME genes (also called NM23). Given the fact that not all NME-encoded proteins are catalytically active NDPKs and that NM23 generally refers to clinical studies on metastasis, we use here NME/NDPK to denote the proteins. Since their discovery in the 1950’s, NMEs/NDPKs have been shown to be involved in multiple physiological and pathological cellular processes, but the molecular mechanisms have not been fully determined. Recent progress in elucidating these underlying mechanisms has been presented by experts in the field at the 10th International Congress on the NDPK/NME/AWD protein family in October 2016 in Dubrovnik, Croatia, and is summarized in review articles or original research in this and an upcoming issue of Laboratory Investigation. Within this editorial, we discuss three major cellular processes that involve members of the multi-functional NME/NDPK family: (i) cancer and metastasis dissemination, (ii) membrane remodeling and nucleotide channeling, and iii) protein histidine phosphorylation. Laboratory Investigation (2018) 98, 164–174; doi:10.1038/labinvest.2017.137 ucleoside diphosphate kinases mitochondria (Lacombe et al, upcoming issue8). (NDPK) are nucleotide metabolism NME1, NME2, NME3, and NME4 form hexamers enzymes encoded by NME (also as the catalytically active form (Ćetković et al, next named NM23) genes. NDPKs are issue9). Group II is comprised of more divergent ubiquitous enzymes catalyzing the proteins sharing only 22 to 44% identity with Ntransfer of a phosphate from nucleoside group I enzymes and between each other.
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Molecular Mechanisms Underlying the Metastasis Suppressor Activity of NME1 in Malignant Melanoma Item Type dissertation Authors Pamidimukkala, Nidhi Vela Publication Date 2019 Abstract Metastatic melanoma is exceedingly lethal and is responsible for the majority of skin cancer related deaths. Impactful research on melanoma metastasis is crucial to improving prognosis. The discovery of metastasis suppressor genes, genes that inhibit... Keywords ALDOC; NME1; Metastasis; Fructose-Bisphosphate Aldolase; Melanoma; Transcription, Genetic Download date 26/09/2021 16:56:05 Link to Item http://hdl.handle.net/10713/9611 Curriculum Vitae Nidhi V. Pamidimukkala [email protected] https://www.linkedin.com/in/nidhi-pamidimukkala Doctor of Philosophy, 2019 EDUCATION 2012-2019 Ph.D., Graduate Program in Molecular Medicine, Cancer Biology Tack University of Maryland School of Medicine – Baltimore, MD 2005-2009 B.S., Biological Sciences, Mellon College of Science Carnegie Mellon University - Pittsburgh, PA 2005-2009 Minor, Chemistry, Mellon College of Science Carnegie Mellon University - Pittsburgh, PA RESEARCH EXPERIENCE 2012-2019 Graduate Research Assistant, Mentor: David M. Kaetzel, Ph.D University of Maryland School of Medicine-Baltimore, MD 2009-2012 Biochemist-MBV Bioprocessing Group Merck & Co, Inc.-Union, NJ 2008-2008 Future Talent Intern-Drug Metabolism Group Merck & Co., Inc- Rahway, NJ 2007-2009 Undergraduate Research Assistant, Mentor: Gordon S. Rule, Ph.D Carnegie Mellon University-Pittsburgh, PA RESEARCH PUBLICATIONS Pamidimukkala, N.V , Leonard, M.K, Snyder, D., McCorkle, J.R., Kaetzel, D.M. (2018). Metastasis Suppressor NME1 Directly Activates Transcription of the ALDOC Gene in Melanoma Cells. Anticancer Research,38(11), 6059-6068. PMID: 30396920 Leonard, M.K, Novak, M., Snyder, D., Snow, G., Pamidimukkala, N.V , McCorkle, J.R., Yang, X.H., Kaetzel, D.M.
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