Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG AARSL alanyl-tRNA synthetase like 672 +/- 50 780 +/- 24 666 +/- 26 777 +/- 38 -0.959643 0.0904189 0.04684 0.0097575 0.0922137 AASDH 2-aminoadipic 6-semialdehyde dehydrogenase 587 +/- 19 771 +/- 21 501 +/- 29 664 +/- 26 -6.83E-05 8.644E-05 0.002318 0.0046175 0.0347906 ABCG2 ATP-binding cassette, sub-family G (WHITE), member 2 437 +/- 47 523 +/- 31 335 +/- 10 398 +/- 11 -0.001844 0.1582204 0.002045 0.1826101 0.0544598 ABHD14A abhydrolase domain containing 14A 1760 +/- 56 1515 +/- 91 1973 +/- 103 1818 +/- 29 0.010547 -0.048824 -0.200935 0.0371071 0.3262792 ABP1 Amiloride binding protein 1 (amine oxidase (copper-containing)) 105 +/- 10 165 +/- 23 154 +/- 17 181 +/- 18 0.021482 0.0527017 0.302632 5.18E-05 0.0770133 ABTB2 Ankyrin repeat and BTB (POZ) domain containing 2 654 +/- 122 485 +/- 39 505 +/- 32 342 +/- 18 -0.030184 -0.236531 -0.002265 0.5021444 0.0832426 ACAA2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) 589 +/- 56 854 +/- 62 529 +/- 31 645 +/- 33 -0.03897 0.0097889 0.030153 0.000594 0.1078687 ACACB acetyl-Coenzyme A carboxylase beta 124 +/- 17 175 +/- 13 138 +/- 12 164 +/- 12 0.943167 0.0376355 0.152219 0.0293754 0.3358733 ACADL acyl-Coenzyme A dehydrogenase, long chain 234 +/- 12 184 +/- 12 226 +/- 9 179 +/- 19 -0.359297 -0.013599 -0.065477 0.0959391 0.0845069 ACAT1 acetyl-Coenzyme A acetyltransferase 1 (acetoacetyl Coenzyme A thiolase) 4099 +/- 188 3515 +/- 106 3250 +/- 137 3238 +/- 137 -0.007467 -0.027046 -0.954273 0.1128106 0.9705821 ACCN1 amiloride-sensitive cation channel 1, neuronal (degenerin) 1925 +/- 136 1421 +/- 164 1179 +/- 59 900 +/- 42 -0.000254 -0.040656 -0.004392 0.0273253 0.0121481 ACIN1 apoptotic chromatin condensation inducer 1 1859 +/- 171 1790 +/- 102 2092 +/- 94 1822 +/- 45 0.172929 -0.735092 -0.03529 0.8562892 0.0993413 ACO1 aconitase 1, soluble 964 +/- 82 811 +/- 43 950 +/- 45 803 +/- 46 -0.754764 -0.139533 -0.048938 0.1183283 0.0289747 ACOT9 acyl-CoA thioesterase 9 370 +/- 33 344 +/- 40 284 +/- 9 218 +/- 20 -0.001741 -0.63831 -0.024981 0.6715461 0.2839666 ACP1 acid phosphatase 1, soluble 2644 +/- 165 2074 +/- 157 2357 +/- 91 2031 +/- 147 -0.085298 -0.031306 -0.10182 0.009087 0.0106821 ACP6 acid phosphatase 6, lysophosphatidic 881 +/- 49 984 +/- 51 742 +/- 28 837 +/- 8 -0.002935 0.171551 0.018151 0.0355159 0.0710112 ACSS1 acyl-CoA synthetase short-chain family member 1 229 +/- 21 311 +/- 28 196 +/- 16 228 +/- 23 -0.013777 0.0442198 0.295382 0.092173 0.4151929 ACTC actin, alpha, cardiac muscle 84 +/- 15 206 +/- 35 98 +/- 11 212 +/- 35 0.021907 0.0155645 0.029736 0.0980108 0.1657953 ACTR10 actin-related protein 10 homolog (S. cerevisiae) 6172 +/- 108 6822 +/- 248 6301 +/- 210 7510 +/- 229 0.046595 0.0483003 0.003988 0.5901922 0.0845271 ACTR1A ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) 3191 +/- 134 2571 +/- 136 2763 +/- 211 2529 +/- 81 -0.241727 -0.008683 -0.339256 0.0015617 0.5937013 ACVR1B activin A receptor, type IB 1767 +/- 140 1532 +/- 52 1675 +/- 66 1338 +/- 98 -0.117997 -0.163381 -0.023761 0.2017275 0.249096 ACY1 aminoacylase 1 493 +/- 28 523 +/- 20 435 +/- 30 520 +/- 22 -0.072713 0.3972113 0.048724 0.6064414 0.155183 ACY1L2 aminoacylase 1-like 2 224 +/- 39 230 +/- 24 450 +/- 41 321 +/- 40 0.001236 0.8951819 -0.050237 0.5937618 0.3209364 ADA adenosine deaminase 265 +/- 19 352 +/- 29 341 +/- 51 373 +/- 25 0.130605 0.0331452 0.593305 0.0330362 0.5864965 ADAM10 ADAM metallopeptidase domain 10 1557 +/- 99 1808 +/- 84 1420 +/- 70 1612 +/- 46 -0.053577 0.0837127 0.049117 0.0627916 0.3649355 ADAM15 ADAM metallopeptidase domain 15 (metargidin) 1022 +/- 24 1299 +/- 94 1046 +/- 106 1001 +/- 50 -0.089311 0.0308546 -0.711056 0.1417549 0.7624836 ADCK4 aarF domain containing kinase 4 430 +/- 24 546 +/- 40 383 +/- 31 503 +/- 38 -0.139029 0.0374119 0.039519 0.2730918 0.306371 ADD1 adducin 1 (alpha) 4262 +/- 135 3716 +/- 140 3921 +/- 116 3527 +/- 163 -0.000231 -0.018613 -0.086088 0.0313122 0.2337141 ADD3 adducin 3 (gamma) 2311 +/- 330 3058 +/- 364 3069 +/- 265 3733 +/- 91 0.020001 0.1598374 0.054267 0.1569602 0.2721863 ADI1 acireductone dioxygenase 1 841 +/- 61 1306 +/- 144 924 +/- 99 1052 +/- 80 -0.633769 0.0213659 0.342331 0.0411467 0.8502112 ADPRH ADP-ribosylarginine hydrolase 189 +/- 23 309 +/- 20 168 +/- 16 281 +/- 20 -0.015382 0.0030101 0.00234 0.0028139 0.0090525 AFF3 AF4/FMR2 family, member 3 128 +/- 17 237 +/- 14 224 +/- 20 358 +/- 44 0.000454 0.0007359 0.034613 0.0013776 0.0003071 AFTIPHILINaftiphilin protein 689 +/- 9 552 +/- 54 739 +/- 50 699 +/- 28 0.034714 -0.050685 -0.502967 0.1353892 0.6185892 AGPAT3 1-acylglycerol-3-phosphate O-acyltransferase 3 124 +/- 10 226 +/- 20 169 +/- 8 232 +/- 26 0.32873 0.0024393 0.066576 0.0077687 0.0848176 AGXT2L1 alanine-glyoxylate aminotransferase 2-like 1 3918 +/- 414 5586 +/- 247 3574 +/- 289 4607 +/- 398 -0.017396 0.0082839 0.070131 0.0534143 0.1478785 AHCYL1 S-adenosylhomocysteine hydrolase-like 1 5935 +/- 600 7815 +/- 704 6059 +/- 511 8209 +/- 330 0.89046 0.0702645 0.007299 0.178801 0.1949046 AHSA1 AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) 3672 +/- 96 3351 +/- 98 3165 +/- 122 3121 +/- 103 -0.010338 -0.04181 -0.788981 0.0745863 0.7081707 AIM1 absent in melanoma 1 368 +/- 97 238 +/- 44 114 +/- 15 175 +/- 22 -0.04556 -0.265509 0.050898 0.4169037 0.3555645 AKAP2 A kinase (PRKA) anchor protein 2 119 +/- 16 187 +/- 25 136 +/- 11 180 +/- 30 0.449089 0.048877 0.230583 0.1043416 0.7033942 AKAP8L A kinase (PRKA) anchor protein 8-like 115 +/- 15 71 +/- 9 120 +/- 16 99 +/- 3 0.145062 -0.038473 -0.237324 0.0940107 0.464845 AKR7A2 aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) 1175 +/- 77 1379 +/- 69 853 +/- 46 1153 +/- 50 -0.000463 0.0781494 0.001841 0.3070654 0.0892846 AKR7A3 aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) 722 +/- 43 917 +/- 15 718 +/- 9 959 +/- 30 0.634132 0.0049548 0.000763 0.0088467 0.0075985 AKT3 V-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) 4120 +/- 100 3342 +/- 250 3311 +/- 184 3045 +/- 90 -0.019216 -0.025143 -0.234272 0.0121962 0.4146659 ALDH3A1 aldehyde dehydrogenase 3 family, memberA1 99 +/- 11 267 +/- 57 115 +/- 22 357 +/- 54 0.192629 0.0317146 0.008136 0.1405832 0.1298736

S1- alphabetical list of aging-related genes.xls Page 1 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG ALDH3B1 aldehyde dehydrogenase 3 family, member B1 147 +/- 9 263 +/- 37 91 +/- 15 124 +/- 5 -0.002691 0.024391 0.084644 0.0284083 0.1508073 ALG5 asparagine-linked glycosylation 5 homolog (S. cerevisiae, dolichyl-phosphate beta-glucosylt 601 +/- 24 662 +/- 22 529 +/- 14 623 +/- 25 -0.004397 0.089144 0.015616 0.0442575 0.0523303 ALKBH8 alkB, alkylation repair homolog 8 (E. coli) 473 +/- 41 658 +/- 40 571 +/- 39 657 +/- 38 0.100357 0.009148 0.144295 0.026047 0.1802512 ALS2CR7 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7 167 +/- 14 134 +/- 8 171 +/- 14 126 +/- 13 -0.89375 -0.070817 -0.048406 0.0693331 0.0090109 AMY1A amylase, alpha 1A; salivary 217 +/- 46 706 +/- 123 210 +/- 34 692 +/- 108 -0.311506 0.0086487 0.008796 0.20853 0.1553613 ANAPC1 anaphase promoting complex subunit 1 942 +/- 48 810 +/- 32 776 +/- 30 736 +/- 25 -0.002459 -0.048252 -0.328608 0.0962622 0.410849 ANAPC10 anaphase promoting complex subunit 10 140 +/- 9 189 +/- 9 212 +/- 8 217 +/- 21 0.001658 0.003833 0.804792 0.0042811 0.1671211 ANAPC11 APC11 anaphase promoting complex subunit 11 homolog (yeast) 1418 +/- 65 1478 +/- 46 1036 +/- 22 1185 +/- 40 -2.42E-05 0.4651226 0.015793 0.9601436 0.2593954 ANAPC13 anaphase promoting complex subunit 13 1133 +/- 61 1351 +/- 62 1288 +/- 122 1286 +/- 100 0.659716 0.0312862 -0.989156 0.0454783 0.4677743 ANGPT1 angiopoietin 1 1046 +/- 93 1388 +/- 95 1366 +/- 231 1407 +/- 71 0.128906 0.0281217 0.870727 0.0085604 0.9933055 ANKH ankylosis, progressive homolog (mouse) 2365 +/- 119 2794 +/- 121 2527 +/- 92 2834 +/- 84 0.309715 0.0303359 0.035778 0.1664892 0.3065043 ANKHD1 ankyrin repeat and KH domain containing 1 870 +/- 60 1083 +/- 30 945 +/- 57 1001 +/- 44 -0.975161 0.0145608 0.45834 0.0060792 0.436999 ANKIB1 ankyrin repeat and IBR domain containing 1 1928 +/- 106 2973 +/- 269 1694 +/- 82 1917 +/- 65 -0.007949 0.009743 0.063334 9E-05 0.0839082 ANKMY1 ankyrin repeat and MYND domain containing 1 80 +/- 6 123 +/- 12 89 +/- 10 110 +/- 19 -0.871513 0.0124915 0.377353 0.0331304 0.1804862 ANKRD10 ankyrin repeat domain 10 912 +/- 51 1028 +/- 46 829 +/- 38 1027 +/- 65 -0.235634 0.116825 0.035519 0.1665156 0.2166634 ANKRD11 ankyrin repeat domain 11 147 +/- 24 240 +/- 28 262 +/- 22 250 +/- 72 0.044845 0.0307121 -0.879094 0.0139103 0.3395989 ANKRD16 ankyrin repeat domain 16 117 +/- 11 155 +/- 15 124 +/- 12 175 +/- 8 0.207757 0.078603 0.005667 0.4099566 0.0824376 ANKRD20Aankyrin repeat domain 20 family, member A1 143 +/- 19 247 +/- 40 57 +/- 20 106 +/- 21 -0.004106 0.0508737 0.130608 0.0214372 0.1929429 ANKRD34 ankyrin repeat domain 34 1681 +/- 72 1443 +/- 63 1781 +/- 88 1714 +/- 34 0.039068 -0.031915 -0.501221 0.0025436 0.8755633 ANKRD37 ankyrin repeat domain 37 571 +/- 66 630 +/- 24 455 +/- 38 593 +/- 37 -0.099949 0.4327419 0.027995 0.4128942 0.0187851 ANKRD43 ankyrin repeat domain 43 1626 +/- 115 1329 +/- 135 1930 +/- 112 1590 +/- 92 0.041881 -0.125069 -0.044003 0.1207345 0.1464073 ANKRD52 ankyrin repeat domain 52 1020 +/- 66 863 +/- 45 1073 +/- 53 917 +/- 18 0.324193 -0.080713 -0.030652 0.1436219 0.1729886 ANKS1B ankyrin repeat and sterile alpha motif domain containing 1B 7067 +/- 478 8852 +/- 493 7058 +/- 279 8534 +/- 485 -0.731046 0.0265886 0.035709 0.0075347 0.1131341 ANP32B acidic (leucine-rich) nuclear phosphoprotein 32 family, member B 5261 +/- 630 6605 +/- 629 4342 +/- 135 4863 +/- 137 -0.020829 0.1620245 0.024182 0.065918 0.0327325 AP2B1 adaptor-related protein complex 2, beta 1 subunit 7691 +/- 354 5572 +/- 446 5527 +/- 121 4060 +/- 189 -0.000127 -0.004297 -0.000323 0.0287975 0.0273872 AP4E1 adaptor-related protein complex 4, epsilon 1 subunit 302 +/- 25 267 +/- 10 347 +/- 7 261 +/- 13 0.134526 -0.231272 -0.000873 0.8974693 0.010348 AP4S1 adaptor-related protein complex 4, sigma 1 subunit 336 +/- 50 195 +/- 7 440 +/- 40 279 +/- 34 0.006951 -0.035594 -0.012958 0.0223472 0.0475868 APBB1 amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) 5027 +/- 303 5203 +/- 381 4570 +/- 115 4883 +/- 59 -0.153539 0.7245922 0.044144 0.539062 0.0316903 APBB1IP amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein 146 +/- 16 200 +/- 16 146 +/- 13 166 +/- 11 -0.405789 0.0351104 0.285013 0.014208 0.6673145 API5 apoptosis inhibitor 5 1659 +/- 24 1825 +/- 54 1812 +/- 42 1906 +/- 76 0.037835 0.0265735 0.322472 0.0189811 0.0551939 APLP1 amyloid beta (A4) precursor-like protein 1 12980 +/- 687 13451 +/- 820 11777 +/- 440 13170 +/- 402 -0.27261 0.6699599 0.044232 0.6211934 0.2032392 APLP2 Amyloid beta (A4) precursor-like protein 2 397 +/- 24 319 +/- 21 448 +/- 28 403 +/- 22 0.010772 -0.037329 -0.240439 0.077951 0.5159072 APOA1BP apolipoprotein A-I binding protein 3444 +/- 146 3736 +/- 191 2627 +/- 74 3301 +/- 117 -0.000886 0.2539505 0.001861 0.2150369 0.0332909 APOD apolipoprotein D 1694 +/- 156 2698 +/- 413 1143 +/- 60 1879 +/- 176 -0.002071 0.0605189 0.011046 0.0199683 0.0099519 APRIN androgen-induced proliferation inhibitor 2075 +/- 34 1723 +/- 57 2125 +/- 61 1961 +/- 40 0.027176 -0.000695 -0.054459 7.079E-06 0.033862 AQP4 aquaporin 4 3429 +/- 247 4405 +/- 244 3780 +/- 277 3499 +/- 199 -0.351243 0.0182687 -0.431325 0.0137531 0.4163361 ARFIP2 ADP-ribosylation factor interacting protein 2 (arfaptin 2) 1246 +/- 34 1120 +/- 36 1244 +/- 43 1245 +/- 60 0.241294 -0.029165 0.986526 0.0071719 0.5121562 ARHGAP1 Rho GTPase activating protein 1 2732 +/- 133 3160 +/- 91 2618 +/- 114 2959 +/- 91 -0.087418 0.0261302 0.045362 0.1377545 0.4245211 ARHGAP2 Rho GTPase activating protein 21 696 +/- 46 796 +/- 50 840 +/- 34 715 +/- 22 0.242334 0.1755294 -0.014326 0.1245424 0.0339893 ARHGAP26Rho GTPase activating protein 26 1897 +/- 171 1395 +/- 80 1867 +/- 154 1491 +/- 130 0.927036 -0.031968 -0.095828 0.0959248 0.1306623 ARHGEF11Rho guanine nucleotide exchange factor (GEF) 11 658 +/- 70 564 +/- 45 969 +/- 43 769 +/- 57 0.000579 -0.290212 -0.023698 0.2544702 0.0498946 ARHGEF12Rho guanine nucleotide exchange factor (GEF) 12 5718 +/- 162 5133 +/- 235 5709 +/- 82 5041 +/- 202 -0.824157 -0.070928 -0.025744 0.01647 0.0003521 ARHGEF2 rho/rac guanine nucleotide exchange factor (GEF) 2 667 +/- 82 848 +/- 50 714 +/- 18 958 +/- 49 0.26721 0.093234 0.005343 0.1031341 0.0226937 ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7 3467 +/- 223 2866 +/- 102 2911 +/- 102 2790 +/- 61 -0.02838 -0.043674 -0.336368 0.0223283 0.410873

S1- alphabetical list of aging-related genes.xls Page 2 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG ARID1B AT rich interactive domain 1B (SWI1-like) 517 +/- 50 742 +/- 58 815 +/- 107 980 +/- 67 0.000873 0.0153366 0.224649 0.1181921 0.401326 ARIH1 Ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) 340 +/- 7 374 +/- 9 372 +/- 17 385 +/- 27 0.253827 0.0120777 0.684705 0.0779694 0.2231039 ARIH2 ariadne homolog 2 (Drosophila) 1295 +/- 30 1073 +/- 49 1029 +/- 41 1044 +/- 54 -0.015864 -0.004513 0.829441 0.1147651 0.7756638 ARL6IP ADP-ribosylation factor-like 6 interacting protein 153 +/- 21 148 +/- 10 183 +/- 10 128 +/- 9 0.490568 -0.810586 -0.002748 0.8348318 0.1231773 ARMC2 armadillo repeat containing 2 247 +/- 26 192 +/- 9 249 +/- 6 220 +/- 6 0.279862 -0.097351 -0.008671 0.1105013 0.0027694 ARMC6 armadillo repeat containing 6 565 +/- 25 466 +/- 18 510 +/- 19 463 +/- 34 -0.266613 -0.009367 -0.270282 0.0009704 0.4644186 ARMCX1 armadillo repeat containing, X-linked 1 1267 +/- 41 1222 +/- 26 1385 +/- 38 1242 +/- 47 0.066419 -0.3784 -0.043796 0.506433 0.3617033 ARPC1B actin related protein 2/3 complex, subunit 1B, 41kDa 265 +/- 16 338 +/- 19 206 +/- 20 235 +/- 20 -0.001183 0.0141484 0.321994 0.0021732 0.7072972 ARPC5L actin related protein 2/3 complex, subunit 5-like 2033 +/- 80 1711 +/- 88 1873 +/- 143 1847 +/- 133 -0.931367 -0.022096 -0.8971 0.0104216 0.878848 ARRB1 arrestin, beta 1 380 +/- 17 456 +/- 27 349 +/- 27 389 +/- 28 -0.156395 0.0424103 0.331062 0.0404324 0.6828249 ARSD arylsulfatase D 461 +/- 56 623 +/- 41 464 +/- 64 612 +/- 50 -0.824537 0.043787 0.101284 0.1024168 0.15605 ASAHL N-acylsphingosine amidohydrolase (acid ceramidase)-like 157 +/- 9 116 +/- 12 192 +/- 21 128 +/- 16 0.066622 -0.026696 -0.038097 0.0595455 0.0329058 ASB6 ankyrin repeat and SOCS box-containing 6 176 +/- 8 150 +/- 8 238 +/- 19 208 +/- 4 6.79E-05 -0.052557 -0.185877 0.1854066 0.3284327 ASB8 ankyrin repeat and SOCS box-containing 8 524 +/- 37 698 +/- 46 630 +/- 54 667 +/- 53 0.160758 0.015618 0.635976 0.0172022 0.3534385 ASF1A ASF1 anti-silencing function 1 homolog A (S. cerevisiae) 389 +/- 32 477 +/- 23 500 +/- 19 435 +/- 10 0.20467 0.0535443 -0.016733 0.0253333 0.0195744 ASPHD2 aspartate beta-hydroxylase domain containing 2 919 +/- 80 730 +/- 79 1141 +/- 46 992 +/- 32 0.002157 -0.122917 -0.027085 0.0684548 0.0557951 ASTN2 Astrotactin 2 149 +/- 10 185 +/- 27 178 +/- 20 260 +/- 21 0.037549 0.2540618 0.021935 0.4993867 0.2857922 ATAD2 ATPase family, AAA domain containing 2 97 +/- 15 148 +/- 7 117 +/- 7 127 +/- 8 -0.891597 0.0181211 0.41145 0.0864308 0.2851105 ATF1 Activating transcription factor 1 660 +/- 47 833 +/- 36 824 +/- 43 894 +/- 69 0.085587 0.0165201 0.413932 0.0171804 0.3602559 ATG12 ATG12 autophagy related 12 homolog (S. cerevisiae) 1361 +/- 96 1868 +/- 140 1316 +/- 125 1632 +/- 120 -0.087718 0.0157577 0.101921 0.0006093 0.020707 ATG4B ATG4 autophagy related 4 homolog B (S. cerevisiae) 489 +/- 25 632 +/- 27 517 +/- 23 734 +/- 11 0.023955 0.0030799 5.64E-05 0.0439593 0.0065325 ATP2C1 ATPase, Ca++ transporting, type 2C, member 1 2029 +/- 118 1514 +/- 111 1497 +/- 112 1332 +/- 42 -0.003401 -0.009807 -0.213381 0.0042259 0.2469229 ATP5D ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit 1719 +/- 90 1425 +/- 89 2012 +/- 103 1836 +/- 84 0.000518 -0.042683 -0.215555 0.1947563 0.6087121 ATP6V1C1ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1 998 +/- 141 706 +/- 35 1092 +/- 143 659 +/- 56 0.489304 -0.094192 -0.027765 0.1672911 0.1605408 ATP6V1D ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D 208 +/- 7 103 +/- 11 218 +/- 21 236 +/- 128 0.419591 -0.000226 0.891766 0.0042581 0.6526729 ATP6V1H ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H 5128 +/- 416 3241 +/- 499 4550 +/- 162 3067 +/- 564 -0.083263 -0.016199 -0.056256 0.0124313 0.0555702 ATP8B2 ATPase, Class I, type 8B, member 2 1260 +/- 36 1102 +/- 43 1508 +/- 74 1279 +/- 39 0.000662 -0.018718 -0.027391 0.0716008 0.1292793 ATPBD1B ATP binding domain 1 family, member B 517 +/- 51 773 +/- 37 492 +/- 40 837 +/- 27 0.822615 0.0029109 7.1E-05 0.0124511 0.0230446 ATXN2 ataxin 2 1690 +/- 104 2226 +/- 102 1668 +/- 61 2193 +/- 86 -0.80154 0.0043761 0.001322 0.0391748 0.0196687 ATXN7L1 ataxin 7-like 1 462 +/- 25 555 +/- 22 612 +/- 31 626 +/- 34 0.003912 0.0190188 0.771269 0.0840158 0.431721 ATXN7L3 ataxin 7-like 3 4182 +/- 141 3742 +/- 124 3753 +/- 122 3589 +/- 83 -0.010413 -0.041159 -0.297757 0.0055341 0.7757953 AURKA aurora kinase A 143 +/- 8 167 +/- 14 108 +/- 7 135 +/- 5 -0.003063 0.1749027 0.017575 0.0328976 0.0511261 AURKAIP1 aurora kinase A interacting protein 1 1086 +/- 137 1595 +/- 169 1097 +/- 103 1678 +/- 137 0.160723 0.0426736 0.009992 0.1630108 0.0713341 AXL AXL receptor tyrosine kinase 951 +/- 51 1108 +/- 50 906 +/- 51 754 +/- 67 -0.01455 0.054106 -0.110761 0.2028006 0.0169621 AYP1 AYP1 protein 1952 +/- 156 2320 +/- 203 1716 +/- 121 2044 +/- 75 -0.127408 0.1834171 0.049916 0.0808976 0.0674348 AZI2 5-azacytidine induced 2 394 +/- 23 458 +/- 16 409 +/- 24 439 +/- 10 -0.660114 0.0482444 0.292643 0.0336147 0.3530425 B2M beta-2-microglobulin 7946 +/- 519 10622 +/- 678 6616 +/- 572 9837 +/- 646 -0.020796 0.0114966 0.005147 0.0423626 0.1051803 B3GNT1 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 7168 +/- 286 6332 +/- 368 7310 +/- 119 6286 +/- 200 0.467301 -0.105163 -0.003595 0.0523104 0.0044193 B3GNTL1 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 215 +/- 11 174 +/- 8 238 +/- 21 202 +/- 7 0.016043 -0.011299 -0.156849 0.0343221 0.3937487 B4GALT3 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3 1509 +/- 46 1313 +/- 50 1304 +/- 35 1196 +/- 29 -0.000177 -0.016366 -0.043079 0.0059715 0.0109205 B4GALT5 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 2214 +/- 209 1568 +/- 99 1896 +/- 165 1568 +/- 81 -0.03683 -0.026376 -0.117469 0.0216119 0.1666359 bA16L21.2 DnaJ-like protein 465 +/- 29 601 +/- 37 472 +/- 48 628 +/- 64 0.563406 0.0162922 0.086858 0.0074342 0.0212148 BAALC brain and acute leukemia, cytoplasmic 5677 +/- 195 4724 +/- 234 5418 +/- 180 4924 +/- 267 -0.971717 -0.01105 -0.167427 0.0070715 0.0303023 BAG1 BCL2-associated athanogene 642 +/- 54 817 +/- 54 829 +/- 57 905 +/- 31 0.029825 0.0459185 0.271393 0.2549285 0.394043

S1- alphabetical list of aging-related genes.xls Page 3 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG BAG5 BCL2-associated athanogene 5 1207 +/- 46 1153 +/- 44 1176 +/- 30 1285 +/- 20 0.249987 -0.41745 0.015127 0.4431129 0.0787352 BAX BCL2-associated X protein 831 +/- 23 877 +/- 24 728 +/- 13 807 +/- 21 -0.000268 0.188443 0.01614 0.3452937 0.1605293 BBS10 Bardet-Biedl syndrome 10 654 +/- 36 800 +/- 32 792 +/- 37 735 +/- 48 0.282967 0.0127777 -0.374216 0.0041161 0.3153397 BBX bobby sox homolog (Drosophila) 1408 +/- 90 1558 +/- 89 1131 +/- 18 1251 +/- 43 -0.00139 0.2635379 0.045523 0.1257724 0.0998663 BCAP31 B-cell receptor-associated protein 31 1029 +/- 88 1543 +/- 54 860 +/- 54 1436 +/- 92 -0.001081 0.0009896 0.001211 0.004477 0.0133562 BCL10 B-cell CLL/lymphoma 10 367 +/- 32 332 +/- 22 459 +/- 20 380 +/- 15 0.010289 -0.395955 -0.011414 0.3654709 0.0460348 BCL9L B-cell CLL/lymphoma 9-like 590 +/- 55 534 +/- 38 851 +/- 35 651 +/- 50 0.002662 -0.417435 -0.012774 0.3931402 0.0874792 BHLHB9 basic helix-loop-helix domain containing, class B, 9 1246 +/- 54 1079 +/- 55 1348 +/- 40 1378 +/- 74 0.00898 -0.054366 0.738951 0.0366155 0.1918358 BICC1 Bicaudal C homolog 1 (Drosophila) 484 +/- 64 390 +/- 42 763 +/- 49 466 +/- 75 0.010564 -0.249579 -0.012458 0.4693602 0.1136697 BICD2 bicaudal D homolog 2 (Drosophila) 1123 +/- 23 818 +/- 85 1051 +/- 110 902 +/- 129 0.056606 -0.014435 -0.404725 0.3535027 0.7955801 BIRC6 baculoviral IAP repeat-containing 6 (apollon) 348 +/- 15 458 +/- 21 431 +/- 7 524 +/- 30 4.21E-05 0.0020667 0.034166 0.000872 0.0185995 BOP1 block of proliferation 1 292 +/- 18 210 +/- 26 357 +/- 15 258 +/- 9 0.005846 -0.026404 -0.000564 0.0936834 0.0022434 BPNT1 3'(2'), 5'-bisphosphate nucleotidase 1 161 +/- 18 269 +/- 23 210 +/- 23 267 +/- 28 0.158484 0.0048843 0.156536 0.0169403 0.0348519 BRCC2 BRCC2 260 +/- 26 370 +/- 35 220 +/- 11 242 +/- 10 -0.012762 0.0323446 0.172532 0.0087585 0.3163604 BRD2 bromodomain containing 2 683 +/- 52 469 +/- 53 945 +/- 41 572 +/- 72 0.004681 -0.016525 -0.003474 0.2800474 0.0970544 BRD8 bromodomain containing 8 2792 +/- 155 3452 +/- 144 2758 +/- 92 3162 +/- 229 -0.266079 0.0110736 0.159508 0.0100204 0.057173 BRWD2 bromodomain and WD repeat domain containing 2 564 +/- 69 382 +/- 3 593 +/- 41 404 +/- 22 0.615269 -0.046177 -0.004233 0.1376207 0.0357696 BTBD12 BTB (POZ) domain containing 12 437 +/- 23 371 +/- 26 440 +/- 20 356 +/- 19 -0.804196 -0.083174 -0.013972 0.1339189 0.2063703 BTBD2 BTB (POZ) domain containing 2 1567 +/- 138 1823 +/- 101 1620 +/- 100 2016 +/- 105 0.152705 0.1687539 0.023564 0.4501454 0.0686026 BTBD6 BTB (POZ) domain containing 6 118 +/- 4 104 +/- 8 134 +/- 8 104 +/- 6 0.312173 -0.15831 -0.015357 0.0606079 0.014503 BTF3 basic transcription factor 3 1055 +/- 87 1438 +/- 101 1121 +/- 86 1351 +/- 104 -0.899068 0.0168246 0.126083 0.001568 0.0303016 BTG4 B-cell translocation gene 4 106 +/- 9 193 +/- 31 105 +/- 8 196 +/- 11 0.585522 0.035256 0.000213 0.0939422 0.0050279 BTN2A2 butyrophilin, subfamily 2, member A2 158 +/- 20 239 +/- 24 168 +/- 18 199 +/- 14 -0.434283 0.0262197 0.201096 0.0493648 0.6504022 BTN3A1 butyrophilin, subfamily 3, member A1 194 +/- 23 323 +/- 36 209 +/- 19 260 +/- 29 -0.381616 0.0163828 0.183902 0.0577965 0.1481924 BUB3 BUB3 budding uninhibited by benzimidazoles 3 homolog (yeast) 4140 +/- 127 3656 +/- 142 3469 +/- 169 3052 +/- 117 -0.000718 -0.02942 -0.074618 0.2495534 0.1460212 BXDC5 brix domain containing 5 1465 +/- 71 1335 +/- 55 1579 +/- 58 1297 +/- 45 0.533917 -0.17978 -0.004051 0.3902844 0.0388022 BZRP benzodiazapine receptor (peripheral) 565 +/- 74 798 +/- 62 374 +/- 21 504 +/- 36 -0.003355 0.0377401 0.018487 0.0214934 0.1014083 BZW1 basic leucine zipper and W2 domains 1 5216 +/- 116 4624 +/- 192 4100 +/- 120 3941 +/- 258 -0.000248 -0.028896 -0.596853 0.0047402 0.2150581 C1QC complement component 1, q subcomponent, C chain 541 +/- 44 951 +/- 109 482 +/- 52 755 +/- 55 -0.028928 0.0110178 0.006202 0.0003612 0.0024066 CA10 carbonic anhydrase X 1854 +/- 361 1852 +/- 301 4062 +/- 174 3455 +/- 172 3.99E-05 -0.996745 -0.035214 0.9563537 0.1060229 CABP4 calcium binding protein 4 167 +/- 42 189 +/- 30 193 +/- 12 142 +/- 16 -0.901635 0.6781443 -0.035053 0.3978992 0.0971859 CABYR calcium binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) 307 +/- 34 240 +/- 11 269 +/- 21 207 +/- 8 -0.089852 -0.109651 -0.032139 0.1049094 0.0171144 CACNA1A calcium channel, voltage-dependent, P/Q type, alpha 1A subunit 961 +/- 89 664 +/- 44 1617 +/- 110 993 +/- 119 0.000194 -0.018645 -0.004193 0.0747641 0.1216223 CACNB1 calcium channel, voltage-dependent, beta 1 subunit 1084 +/- 79 825 +/- 44 1214 +/- 70 1044 +/- 36 0.009523 -0.021979 -0.065895 0.0056008 0.145536 CALCOCO calcium binding and coiled-coil domain 1 1651 +/- 65 1696 +/- 88 1450 +/- 9 1669 +/- 54 -0.151045 0.6944536 0.014307 0.3147448 0.1743436 CALCOCO calcium binding and coiled-coil domain 2 135 +/- 14 182 +/- 16 231 +/- 15 267 +/- 26 0.000718 0.0542604 0.274321 0.0064576 0.2571646 CALD1 caldesmon 1 84 +/- 6 130 +/- 15 132 +/- 8 136 +/- 6 0.005626 0.0251705 0.710226 0.0022 0.752043 CAMKK2 calcium/calmodulin-dependent protein kinase kinase 2, beta 2835 +/- 306 2109 +/- 116 2217 +/- 96 1716 +/- 125 -0.024082 -0.065712 -0.012947 0.0168465 0.0277705 CAMSAP1 calmodulin regulated spectrin-associated protein 1 1864 +/- 55 1556 +/- 68 1653 +/- 70 1442 +/- 53 -0.000838 -0.005929 -0.039507 0.036372 0.1589263 CAMTA1 calmodulin binding transcription activator 1 3071 +/- 288 2648 +/- 114 3284 +/- 169 2656 +/- 62 0.5964 -0.218065 -0.011943 0.1678939 0.0245317 CAPN2 calpain 2, (m/II) large subunit 2440 +/- 204 3016 +/- 132 1931 +/- 137 2492 +/- 70 -0.000223 0.0430506 0.00757 0.0562623 0.0767471 CAPN7 calpain 7 580 +/- 29 674 +/- 27 693 +/- 37 800 +/- 39 0.004627 0.039422 0.081336 0.0466246 0.266771 CAPNS1 calpain, small subunit 1 7708 +/- 486 6177 +/- 323 7211 +/- 399 6252 +/- 282 -0.353239 -0.028383 -0.082827 0.0128554 0.156197 CAPZB capping protein (actin filament) muscle Z-line, beta 8247 +/- 582 6857 +/- 252 6783 +/- 248 5959 +/- 245 -0.006127 -0.065542 -0.0425 0.1260651 0.0938284

S1- alphabetical list of aging-related genes.xls Page 4 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG CARD9 caspase recruitment domain family, member 9 119 +/- 10 107 +/- 15 126 +/- 10 91 +/- 8 -0.759742 -0.509165 -0.024558 0.6904611 0.1781829 CARF collaborates/cooperates with ARF (alternate reading frame) protein 269 +/- 10 254 +/- 13 331 +/- 17 273 +/- 18 0.037888 -0.405664 -0.042435 0.9848762 0.2328383 CASP6 Caspase 6, apoptosis-related cysteine peptidase 345 +/- 21 260 +/- 29 301 +/- 24 283 +/- 18 -0.233104 -0.040368 -0.566645 0.0229999 0.2626178 CAST calpastatin 990 +/- 127 1424 +/- 112 822 +/- 41 1048 +/- 19 -0.011634 0.0287645 0.00158 0.0161794 0.0076301 CAST1 CAZ-associated structural protein 3281 +/- 360 2648 +/- 269 4391 +/- 181 3526 +/- 194 0.000442 -0.19195 -0.010196 0.208326 0.0276119 CBARA1 calcium binding atopy-related autoantigen 1 1698 +/- 52 1549 +/- 37 1445 +/- 72 1507 +/- 58 -0.017109 -0.04407 0.517737 0.1406721 0.701145 CBL Cas-Br-M (murine) ecotropic retroviral transforming sequence 1129 +/- 118 865 +/- 92 1148 +/- 81 844 +/- 74 -0.828443 -0.109053 -0.021736 0.1314337 0.0102932 CBR4 Carbonyl reductase 4 86 +/- 10 138 +/- 8 105 +/- 19 109 +/- 10 -0.882959 0.0021832 0.871819 0.0006885 0.9016726 CBX3 chromobox homolog 3 (HP1 gamma homolog, Drosophila) 2091 +/- 138 2460 +/- 88 2478 +/- 74 2807 +/- 90 0.000466 0.0528484 0.021609 0.0976501 0.0574181 CCAR1 Cell division cycle and apoptosis regulator 1 149 +/- 19 225 +/- 17 200 +/- 14 216 +/- 12 0.277002 0.0143201 0.403649 0.0202498 0.8580945 CCDC109Acoiled-coil domain containing 109A 569 +/- 44 445 +/- 19 621 +/- 31 560 +/- 29 0.009885 -0.036178 -0.189661 0.0297814 0.12063 CCDC28A coiled-coil domain containing 28A 2372 +/- 140 2508 +/- 155 2361 +/- 84 2730 +/- 55 0.347593 0.5291304 0.005847 0.829815 0.0865784 CCDC44 coiled-coil domain containing 44 792 +/- 36 669 +/- 27 578 +/- 22 598 +/- 31 -0.00364 -0.021618 0.607391 0.0106531 0.7380396 CCDC46 coiled-coil domain containing 46 98 +/- 13 159 +/- 31 208 +/- 13 282 +/- 17 0.000554 0.1194535 0.009056 0.2527666 0.1569679 CCDC49 coiled-coil domain containing 49 276 +/- 18 313 +/- 20 246 +/- 12 319 +/- 21 -0.229076 0.2021114 0.019118 0.0975286 0.0471031 CCDC72 coiled-coil domain containing 72 4579 +/- 407 4039 +/- 229 3214 +/- 212 2593 +/- 139 -0.000493 -0.281026 -0.038621 0.5045182 0.0746598 CCDC74A coiled-coil domain containing 74A 613 +/- 51 646 +/- 93 640 +/- 36 1150 +/- 118 0.030966 0.7647319 0.010155 0.4369118 0.0075341 CCDC8 coiled-coil domain containing 8 394 +/- 24 308 +/- 20 374 +/- 23 310 +/- 19 -0.800822 -0.020152 -0.059179 0.1357534 0.1935142 CCDC84 coiled-coil domain containing 84 574 +/- 64 492 +/- 41 476 +/- 29 359 +/- 41 -0.004905 -0.311384 -0.049202 0.5339314 0.2964875 CCDC85A coiled-coil domain containing 85A 92 +/- 15 41 +/- 10 189 +/- 13 169 +/- 26 9.68E-05 -0.0203 -0.516734 0.0450543 0.794482 CCDC86 coiled-coil domain containing 86 471 +/- 31 440 +/- 12 478 +/- 9 411 +/- 12 -0.745525 -0.386079 -0.002237 0.3407222 0.0276324 CCDC95 coiled-coil domain containing 95 936 +/- 37 859 +/- 28 1082 +/- 28 950 +/- 21 0.002123 -0.12682 -0.004763 0.0487537 0.0046589 CCDC98 coiled-coil domain containing 98 121 +/- 10 163 +/- 15 95 +/- 14 126 +/- 13 -0.036337 0.0442752 0.141438 0.0179696 0.319486 CCDC99 Coiled-coil domain containing 99 843 +/- 108 1220 +/- 192 518 +/- 27 688 +/- 24 -0.003641 0.1255292 0.001168 0.0422772 0.0247073 CCL27 chemokine (C-C motif) ligand 27 350 +/- 36 242 +/- 23 335 +/- 21 227 +/- 16 -0.621527 -0.034547 -0.002895 0.0368565 0.0037534 CCNA1 cyclin A1 165 +/- 10 276 +/- 37 154 +/- 14 177 +/- 31 -0.03939 0.0301126 0.505994 0.0100349 0.5542809 CCNDBP1 cyclin D-type binding-protein 1 1466 +/- 70 1579 +/- 106 1288 +/- 57 1562 +/- 89 -0.009416 0.3968733 0.035355 0.750233 0.1385804 CCNF cyclin F 133 +/- 13 125 +/- 9 143 +/- 17 196 +/- 13 0.025337 -0.623944 0.034926 0.3664781 0.1488046 CCRN4L CCR4 carbon catabolite repression 4-like (S. cerevisiae) 357 +/- 52 222 +/- 9 332 +/- 36 323 +/- 35 0.395085 -0.048061 -0.855937 0.0351474 0.6185892 CCT3 chaperonin containing TCP1, subunit 3 (gamma) 2068 +/- 138 2872 +/- 102 1999 +/- 97 2727 +/- 72 -0.051148 0.0010854 0.000218 0.0758965 0.0618846 CCT7 chaperonin containing TCP1, subunit 7 (eta) 926 +/- 48 785 +/- 35 940 +/- 51 822 +/- 54 0.409169 -0.039817 -0.146651 0.0916866 0.8382603 CD2BP2 CD2 (cytoplasmic tail) binding protein 2 434 +/- 27 533 +/- 24 382 +/- 9 508 +/- 28 -0.052734 0.0224622 0.007719 0.1918141 0.0223613 CD44 CD44 molecule (Indian blood group) 390 +/- 49 579 +/- 97 642 +/- 74 919 +/- 44 0.000529 0.1243777 0.012964 0.2130658 0.0386421 CD47 CD47 molecule 2438 +/- 231 1877 +/- 56 2572 +/- 107 2131 +/- 78 0.068634 -0.059507 -0.009186 0.1748846 0.0110473 CD5 CD5 molecule 143 +/- 6 90 +/- 18 125 +/- 19 122 +/- 17 0.702718 -0.034031 -0.906423 0.2326028 0.6804172 CD52 CD52 molecule 303 +/- 58 621 +/- 52 251 +/- 51 388 +/- 37 -0.01515 0.0023325 0.057454 0.0100072 0.2205889 CD58 CD58 molecule 179 +/- 17 277 +/- 45 140 +/- 9 195 +/- 13 -0.021073 0.0842023 0.010655 0.0174593 0.0055731 CD63 CD63 molecule 3961 +/- 565 4903 +/- 297 3173 +/- 139 3664 +/- 165 -0.011074 0.1803626 0.050996 0.198092 0.4036024 CD74 CD74 molecule, major histocompatibility complex, class II invariant chain 1810 +/- 283 3714 +/- 547 1215 +/- 171 2317 +/- 340 -0.016033 0.016153 0.027629 0.0079133 0.1049361 CD81 CD81 molecule 13811 +/- 551 15425 +/- 255 12732 +/- 477 14927 +/- 743 -0.099974 0.0323751 0.041803 0.1944335 0.4250111 CD99 CD99 molecule 895 +/- 110 1907 +/- 171 1138 +/- 143 2051 +/- 28 0.143353 0.0009003 0.001141 0.0008603 0.0179078 CDC16 CDC16 cell division cycle 16 homolog (S. cerevisiae) 1071 +/- 33 1180 +/- 23 991 +/- 39 1063 +/- 17 -8.94E-05 0.0248663 0.13443 0.1310045 0.324503 CDC25A cell division cycle 25A 235 +/- 35 330 +/- 18 224 +/- 25 264 +/- 25 -0.041807 0.0419309 0.285905 0.2069823 0.6622406 CDC42EP1CDC42 effector protein (Rho GTPase binding) 1 483 +/- 70 667 +/- 95 446 +/- 30 548 +/- 33 -0.229542 0.1502334 0.047948 0.0722411 0.0967348

S1- alphabetical list of aging-related genes.xls Page 5 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG CDH24 cadherin-like 24 969 +/- 98 925 +/- 70 890 +/- 56 690 +/- 25 -0.060269 -0.726266 -0.014013 0.6436917 0.0086368 CDIPT CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase) 3358 +/- 206 3149 +/- 168 3343 +/- 117 2839 +/- 95 -0.40566 -0.451126 -0.008778 0.3661446 0.0872679 CDK5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35) 3649 +/- 206 3034 +/- 85 4001 +/- 116 3504 +/- 169 0.04031 -0.029702 -0.043974 0.0157937 0.3073854 CDK5RAP3CDK5 regulatory subunit associated protein 3 2040 +/- 139 2326 +/- 68 1983 +/- 100 2344 +/- 76 -0.912757 0.1059012 0.018858 0.2761314 0.1281429 CDKL1 Cyclin-dependent kinase-like 1 (CDC2-related kinase) 246 +/- 38 126 +/- 18 235 +/- 31 154 +/- 10 0.544474 -0.02498 -0.045552 0.0374047 0.079404 CDKN2D cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) 1595 +/- 115 1240 +/- 71 1433 +/- 53 1337 +/- 51 -0.531545 -0.029208 -0.225029 0.0016399 0.2668989 CDO1 cysteine dioxygenase, type I 3039 +/- 285 2226 +/- 153 2318 +/- 87 2399 +/- 90 -0.153675 -0.037331 0.537165 0.0164488 0.9143342 CDS1 CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 2137 +/- 201 1588 +/- 146 1255 +/- 83 1183 +/- 110 -0.001244 -0.054361 -0.618529 0.0098995 0.1633426 CENPJ centromere protein J 96 +/- 18 264 +/- 61 97 +/- 12 311 +/- 82 0.110635 0.0401782 0.058315 0.0072099 0.0049012 CEP170 centrosomal protein 170kDa 1001 +/- 123 1338 +/- 117 981 +/- 72 1209 +/- 49 -0.23448 0.0753206 0.029525 0.1319576 0.3466277 CEP27 centrosomal protein 27kDa 292 +/- 19 350 +/- 15 317 +/- 12 384 +/- 28 0.123998 0.0392293 0.075453 0.0522933 0.4491202 CEP63 centrosomal protein 63kDa 153 +/- 12 107 +/- 16 136 +/- 25 82 +/- 13 -0.08455 -0.045694 -0.097117 0.4379545 0.3680895 CEPT1 choline/ethanolamine phosphotransferase 1 157 +/- 10 233 +/- 10 158 +/- 12 186 +/- 14 -0.098759 0.0003531 0.177277 0.0027786 0.2984887 CES2 carboxylesterase 2 (intestine, liver) 171 +/- 23 239 +/- 16 196 +/- 22 188 +/- 19 -0.767725 0.0366105 -0.781637 0.006473 0.9958296 CETN3 centrin, EF-hand protein, 3 (CDC31 homolog, yeast) 1063 +/- 34 1071 +/- 46 1089 +/- 38 984 +/- 18 -0.731738 0.8895652 -0.040433 0.2173904 0.2261738 CFL2 cofilin 2 (muscle) 1198 +/- 120 1807 +/- 334 1128 +/- 106 1503 +/- 117 -0.245685 0.1350858 0.042518 0.017426 0.0658383 CGI-38 brain specific protein 1185 +/- 160 1693 +/- 328 699 +/- 44 901 +/- 69 -0.01281 0.2043037 0.04339 0.1728503 0.1358712 CHCHD3 coiled-coil-helix-coiled-coil-helix domain containing 3 2026 +/- 56 2199 +/- 30 2231 +/- 90 2360 +/- 104 0.004669 0.0263417 0.377636 0.0127559 0.5219367 CHCHD4 coiled-coil-helix-coiled-coil-helix domain containing 4 566 +/- 39 497 +/- 32 476 +/- 27 398 +/- 11 -9.49E-05 -0.201579 -0.032704 0.2495831 0.0645877 CHCHD7 coiled-coil-helix-coiled-coil-helix domain containing 7 1443 +/- 50 1660 +/- 71 1485 +/- 74 1658 +/- 34 0.228951 0.0341582 0.071556 0.1225051 0.1170426 ChGn Chondroitin beta1,4 N-acetylgalactosaminyltransferase 117 +/- 12 160 +/- 11 80 +/- 9 120 +/- 16 -0.010554 0.0304215 0.074225 0.0509525 0.0348366 CHKA choline kinase alpha 607 +/- 41 586 +/- 44 764 +/- 36 646 +/- 32 0.030471 -0.738949 -0.035292 0.8615465 0.0564405 CHL1 cell adhesion molecule with homology to L1CAM (close homolog of L1) 5666 +/- 319 6673 +/- 332 5043 +/- 360 5085 +/- 214 -0.004389 0.0537679 0.922984 0.26906 0.9666862 CHM choroideremia (Rab escort protein 1) 1904 +/- 130 2177 +/- 95 1862 +/- 132 2272 +/- 98 0.989552 0.1232673 0.035472 0.0876603 0.0370802 CHST3 carbohydrate (chondroitin 6) sulfotransferase 3 639 +/- 84 773 +/- 125 385 +/- 5 482 +/- 26 -0.005146 0.3983593 0.019465 0.1758969 0.0445424 CHSY1 carbohydrate (chondroitin) synthase 1 921 +/- 62 844 +/- 26 927 +/- 31 833 +/- 17 -0.880999 -0.289489 -0.031812 0.4493441 0.0670205 CHURC1 churchill domain containing 1 2879 +/- 78 3384 +/- 118 2472 +/- 75 2873 +/- 90 -0.000854 0.006514 0.008507 0.0013983 0.0100486 CIC capicua homolog (Drosophila) 2030 +/- 162 2055 +/- 125 3575 +/- 166 3066 +/- 138 3.82E-05 0.9079011 -0.042879 0.6272452 0.1751269 CKAP1 cytoskeleton associated protein 1 1486 +/- 142 1105 +/- 70 1406 +/- 119 962 +/- 130 -0.374152 -0.045645 -0.033776 0.2838992 0.2017971 CKLF chemokine-like factor 686 +/- 32 579 +/- 45 611 +/- 22 492 +/- 40 -0.011824 -0.086706 -0.037465 0.2042547 0.0197104 CKMT1B creatine kinase, mitochondrial 1B 6947 +/- 213 5592 +/- 354 5522 +/- 246 4526 +/- 212 -0.00021 -0.010828 -0.013362 0.013891 0.1270608 CKS1B CDC28 protein kinase regulatory subunit 1B 822 +/- 24 945 +/- 35 686 +/- 28 763 +/- 14 -0.000153 0.0180419 0.041455 0.0451127 0.2475416 CLCC1 chloride channel CLIC-like 1 332 +/- 29 441 +/- 25 310 +/- 14 395 +/- 5 -0.051789 0.0179946 0.001145 0.066525 0.011344 CLDN15 claudin 15 352 +/- 48 618 +/- 92 272 +/- 21 393 +/- 56 -0.005536 0.0355177 0.099515 0.0144859 0.0386216 CLEC14A C-type lectin domain family 14, member A 139 +/- 9 108 +/- 10 129 +/- 11 111 +/- 13 -0.617085 -0.040997 -0.334514 0.2255612 0.8329779 CLEC4A C-type lectin domain family 4, member A 101 +/- 9 155 +/- 10 108 +/- 13 131 +/- 18 -0.499229 0.0027728 0.334357 0.0041785 0.8807759 CLK4 CDC-like kinase 4 2680 +/- 52 2262 +/- 87 2336 +/- 119 1847 +/- 72 -1.37E-05 -0.003237 -0.00787 0.0890823 0.0935707 CLN3 ceroid-lipofuscinosis, neuronal 3, juvenile (Batten, Spielmeyer-Vogt disease) 69 +/- 19 166 +/- 24 72 +/- 18 167 +/- 36 0.962825 0.0104739 0.059108 0.1621091 0.4560878 CLUAP1 clusterin associated protein 1 364 +/- 28 271 +/- 19 359 +/- 25 394 +/- 15 0.11188 -0.021404 0.266993 0.0150513 0.3768423 CMTM3 CKLF-like MARVEL transmembrane domain containing 3 684 +/- 73 783 +/- 21 553 +/- 20 645 +/- 18 -0.004933 0.2413487 0.007506 0.1880707 0.0672783 CMTM5 CKLF-like MARVEL transmembrane domain containing 5 2631 +/- 293 3487 +/- 483 1346 +/- 59 1861 +/- 191 -0.000853 0.1669512 0.051693 0.069623 0.0709191 CNDP1 carnosine dipeptidase 1 (metallopeptidase M20 family) 1872 +/- 548 3446 +/- 569 974 +/- 183 2032 +/- 172 -0.014765 0.0742997 0.002279 0.0431478 0.006867 CNNM3 Cyclin M3 133 +/- 9 173 +/- 11 136 +/- 17 194 +/- 15 0.464066 0.0157123 0.030455 0.0124335 0.0476581 CNOT1 CCR4-NOT transcription complex, subunit 1 385 +/- 33 315 +/- 9 372 +/- 26 289 +/- 15 -0.133131 -0.091373 -0.022823 0.1665445 0.0437044

S1- alphabetical list of aging-related genes.xls Page 6 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG CNP 2',3'-cyclic nucleotide 3' phosphodiesterase 8116 +/- 1232 11141 +/- 2317 4506 +/- 307 5837 +/- 379 -0.0058 0.2839787 0.025495 0.0954527 0.0080065 CNTN1 contactin 1 1106 +/- 69 1435 +/- 87 1088 +/- 58 1302 +/- 69 -0.267859 0.0149104 0.043579 0.0089787 0.2068081 CNTNAP2 contactin associated protein-like 2 4333 +/- 298 3178 +/- 404 3580 +/- 316 3606 +/- 414 -0.141527 -0.046274 0.961217 0.0642769 0.6630139 CNTNAP4 contactin associated protein-like 4 870 +/- 94 1300 +/- 106 849 +/- 67 946 +/- 93 -0.138096 0.0126049 0.424398 0.0022063 0.720818 COG2 Component of oligomeric golgi complex 2 120 +/- 4 171 +/- 15 148 +/- 17 181 +/- 22 0.046117 0.0163734 0.26106 0.035707 0.4117899 COL11A2 collagen, type XI, alpha 2 262 +/- 34 231 +/- 38 308 +/- 19 229 +/- 25 0.500008 -0.556716 -0.038334 0.7123472 0.2080446 COL1A2 collagen, type I, alpha 2 214 +/- 68 127 +/- 38 73 +/- 10 162 +/- 21 -0.348965 -0.29204 0.010048 0.1030026 0.0184288 COL4A3 collagen, type IV, alpha 3 (Goodpasture antigen) 155 +/- 10 113 +/- 13 122 +/- 13 100 +/- 15 -0.148307 -0.027497 -0.29067 0.0468679 0.1000085 COL8A1 Collagen, type VIII, alpha 1 97 +/- 13 150 +/- 14 69 +/- 14 113 +/- 15 -0.023159 0.0164826 0.061623 0.0556603 0.0055949 COMT catechol-O-methyltransferase 2761 +/- 143 3402 +/- 147 2879 +/- 124 3569 +/- 121 0.377137 0.0106563 0.003185 0.0050392 0.0255997 COPB coatomer protein complex, subunit beta 2361 +/- 106 2533 +/- 108 2354 +/- 49 2670 +/- 101 0.534294 0.2794018 0.030977 0.6419051 0.2110135 COPS6 COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) 4767 +/- 72 4463 +/- 89 4638 +/- 109 4477 +/- 61 -0.502475 -0.024833 -0.232491 0.0048395 0.3377716 COPS7A COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis) 2132 +/- 50 2760 +/- 207 2135 +/- 183 2910 +/- 174 0.733772 0.0276805 0.013514 0.3529859 0.252015 COPS8 COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis) 3844 +/- 259 2904 +/- 248 3403 +/- 232 3169 +/- 263 -0.338675 -0.025702 -0.521506 0.0037733 0.1955366 COQ3 coenzyme Q3 homolog, methyltransferase (S. cerevisiae) 962 +/- 18 1137 +/- 65 967 +/- 22 1098 +/- 25 -0.817125 0.0430001 0.003824 0.3347873 0.1330829 CORO7 coronin 7 1060 +/- 57 830 +/- 78 1137 +/- 43 970 +/- 97 0.053474 -0.040219 -0.170909 0.1279173 0.5328214 COTL1 coactosin-like 1 (Dictyostelium) 2231 +/- 123 2629 +/- 248 2186 +/- 97 2676 +/- 151 0.623129 0.1921305 0.0291 0.1506062 0.0805398 COX4NB COX4 neighbor 1259 +/- 72 1292 +/- 57 1207 +/- 23 1340 +/- 34 -0.99451 0.7250127 0.012696 0.9854078 0.2731861 COX5B cytochrome c oxidase subunit Vb 6825 +/- 788 9221 +/- 528 4736 +/- 885 7292 +/- 122 -3.18E-05 0.0331089 0.03388 0.0665571 0.0655704 COX6C cytochrome c oxidase subunit VIc 7080 +/- 300 8976 +/- 497 6419 +/- 237 8142 +/- 260 -0.027156 0.0110211 0.000959 0.2042941 0.0616353 CPSF2 cleavage and polyadenylation specific factor 2, 100kDa 526 +/- 47 668 +/- 60 737 +/- 35 880 +/- 49 0.000273 0.0943332 0.046656 0.0480927 0.0324146 CRAMP1L Crm, cramped-like (Drosophila) 799 +/- 22 1083 +/- 81 759 +/- 43 853 +/- 75 -0.02985 0.0158006 0.319566 0.109389 0.8164495 CREBBP CREB binding protein (Rubinstein-Taybi syndrome) 1537 +/- 76 1340 +/- 29 1670 +/- 25 1368 +/- 90 0.074631 -0.047722 -0.026138 0.2182167 0.2380851 CRI1 CREBBP/EP300 inhibitor 1 2595 +/- 106 2983 +/- 108 2958 +/- 141 2891 +/- 157 0.256251 0.0281349 -0.757243 0.0258302 0.9845123 CRI2 CREBBP/EP300 inhibitor 2 2677 +/- 69 2462 +/- 64 2961 +/- 148 2988 +/- 133 0.001303 -0.045566 0.896077 0.0134155 0.9560909 CRKL v-crk sarcoma virus CT10 oncogene homolog (avian)-like 1767 +/- 44 1717 +/- 67 1489 +/- 11 1401 +/- 15 -2.12E-05 -0.547481 -0.001865 0.9929446 0.0092549 CRNKL1 Crn, crooked neck-like 1 (Drosophila) 267 +/- 89 512 +/- 56 270 +/- 68 595 +/- 45 0.440492 0.0464274 0.003683 0.1024379 0.0595626 CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa 197 +/- 12 461 +/- 85 228 +/- 18 391 +/- 98 -0.925572 0.0257398 0.174308 0.0019702 0.0537538 CRYBA2 crystallin, beta A2 197 +/- 9 153 +/- 13 189 +/- 23 170 +/- 6 0.902374 -0.022757 -0.458026 0.0053405 0.7632027 CRYZL1 crystallin, zeta (quinone reductase)-like 1 1205 +/- 66 921 +/- 78 933 +/- 90 851 +/- 78 -0.062757 -0.019966 -0.505096 0.0043641 0.4585828 CSF2RB colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) 151 +/- 7 191 +/- 15 133 +/- 12 133 +/- 8 -0.007943 0.0451243 -0.994117 0.0008691 0.7750291 CSNK1D casein kinase 1, delta 809 +/- 36 702 +/- 20 866 +/- 60 764 +/- 92 0.264375 -0.031334 -0.386764 0.02211 0.3344333 CSNK1G2 casein kinase 1, gamma 2 464 +/- 48 332 +/- 27 622 +/- 27 496 +/- 28 0.001863 -0.044697 -0.010409 0.0355452 0.0068422 CSPP1 centrosome and spindle pole associated protein 1 281 +/- 13 401 +/- 33 322 +/- 36 395 +/- 48 0.278161 0.0126706 0.257417 0.0001077 0.0824303 CSTB cystatin B (stefin B) 6756 +/- 454 7724 +/- 450 6112 +/- 199 7133 +/- 147 -0.030487 0.1610221 0.002746 0.2614149 0.0407972 CTDP1 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1 433 +/- 12 380 +/- 8 395 +/- 17 398 +/- 24 -0.558682 -0.006234 0.926065 0.0463695 0.9418725 CTDSP2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 2715 +/- 105 2657 +/- 150 2420 +/- 54 2153 +/- 92 -0.000546 -0.758013 -0.04211 0.7117736 0.1471537 CTNNB1 Catenin (cadherin-associated protein), beta 1, 88kDa 80 +/- 20 240 +/- 38 98 +/- 21 283 +/- 59 0.202088 0.0064958 0.031256 0.0103942 0.0012334 CTSL cathepsin L 1295 +/- 116 1543 +/- 70 1117 +/- 86 1413 +/- 26 -0.105945 0.1024043 0.01727 0.2179043 0.1827795 CTSS cathepsin S 157 +/- 19 238 +/- 20 157 +/- 21 194 +/- 33 -0.257341 0.0137798 0.371198 0.0343246 0.5160115 CTTNBP2 cortactin binding protein 2 537 +/- 51 438 +/- 37 539 +/- 47 360 +/- 32 -0.362999 -0.154618 -0.012403 0.3916878 0.0872516 CUGBP2 CUG triplet repeat, RNA binding protein 2 1263 +/- 229 2289 +/- 319 1772 +/- 270 3161 +/- 347 0.008986 0.028023 0.013492 0.2405286 0.0448164 CUL5 cullin 5 2810 +/- 145 3330 +/- 140 3029 +/- 94 3332 +/- 99 0.370153 0.0273875 0.054928 0.0355529 0.0386436 CWF19L1 CWF19-like 1, cell cycle control (S. pombe) 145 +/- 13 207 +/- 12 192 +/- 13 214 +/- 15 0.140158 0.0065434 0.297556 0.0100324 0.9857923

S1- alphabetical list of aging-related genes.xls Page 7 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG CWF19L2 CWF19-like 2, cell cycle control (S. pombe) 477 +/- 43 740 +/- 86 434 +/- 31 541 +/- 30 -0.053544 0.0270477 0.035306 0.0017024 0.0346302 CXADR Coxsackie virus and adenovirus receptor 2582 +/- 321 1752 +/- 139 2109 +/- 65 1507 +/- 97 -0.07616 -0.050205 -0.00121 0.0443685 0.0132856 CXXC4 CXXC finger 4 336 +/- 31 432 +/- 16 266 +/- 21 359 +/- 24 -0.004324 0.0253939 0.01767 0.098273 0.053262 CYB561 cytochrome b-561 156 +/- 15 132 +/- 15 162 +/- 7 131 +/- 10 0.939814 -0.284122 -0.034607 0.4861141 0.1017238 CYCS cytochrome c, somatic 1114 +/- 69 1523 +/- 105 1124 +/- 93 1485 +/- 52 -0.99493 0.0103416 0.010191 0.1836039 0.1321904 CYP2U1 cytochrome P450, family 2, subfamily U, polypeptide 1 588 +/- 29 675 +/- 23 522 +/- 14 543 +/- 13 -0.000203 0.0432777 0.29925 0.0939982 0.3473348 CYR61 cysteine-rich, angiogenic inducer, 61 179 +/- 15 130 +/- 13 126 +/- 20 128 +/- 12 -0.105469 -0.034656 0.939443 0.0150256 0.8075061 D21S2056EDNA segment on chromosome 21 (unique) 2056 expressed sequence 941 +/- 105 1138 +/- 59 786 +/- 44 1023 +/- 84 -0.012674 0.1404877 0.04602 0.2599288 0.1698107 DAK dihydroxyacetone kinase 2 homolog (S. cerevisiae) 169 +/- 11 116 +/- 13 159 +/- 13 126 +/- 13 -0.883429 -0.009758 -0.113635 0.007527 0.0237072 DBF4B DBF4 homolog B (S. cerevisiae) 88 +/- 14 172 +/- 19 93 +/- 13 170 +/- 27 0.81417 0.0066193 0.043191 0.0433228 0.0069601 DBNDD2 dysbindin (dystrobrevin binding protein 1) domain containing 2 6719 +/- 435 8157 +/- 929 5664 +/- 297 6675 +/- 313 -0.029077 0.2029886 0.044247 0.0499254 0.0076164 DBT dihydrolipoamide branched chain transacylase E2 462 +/- 33 608 +/- 51 504 +/- 30 557 +/- 59 -0.412108 0.0398338 0.4561 0.1623017 0.9693144 DCAMKL2 doublecortin and CaM kinase-like 2 1543 +/- 280 1165 +/- 201 1470 +/- 91 1163 +/- 35 -0.698962 -0.30095 -0.017705 0.4026001 0.0526574 DCTN4 dynactin 4 (p62) 2167 +/- 114 1786 +/- 115 2057 +/- 72 1769 +/- 87 -0.235129 -0.039592 -0.033853 0.4393145 0.3276353 DCTN6 dynactin 6 2538 +/- 127 2919 +/- 63 2668 +/- 126 2992 +/- 196 0.155899 0.0299498 0.207326 0.24043 0.7900069 DCUN1D1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) 2306 +/- 41 1936 +/- 62 1971 +/- 52 1884 +/- 74 -0.002173 -0.00083 -0.365085 0.000397 0.2125893 DDB2 damage-specific DNA binding protein 2, 48kDa 98 +/- 11 160 +/- 9 88 +/- 9 129 +/- 8 -0.122515 0.0015318 0.005825 0.0023322 0.0568833 DDHD1 DDHD domain containing 1 1480 +/- 235 2265 +/- 180 1320 +/- 221 1767 +/- 178 -0.001847 0.0253805 0.151224 0.1292407 0.5710365 DDX48 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 1505 +/- 88 1696 +/- 45 1343 +/- 72 1561 +/- 54 -0.030789 0.0917214 0.038878 0.0217036 0.228826 DDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 600 +/- 31 706 +/- 25 583 +/- 35 620 +/- 21 -0.077657 0.0259954 0.387283 0.0095351 0.5212467 DEDD2 death effector domain containing 2 610 +/- 25 569 +/- 23 643 +/- 21 576 +/- 15 0.190941 -0.256153 -0.027965 0.8402068 0.2988356 DENND1C DENN/MADD domain containing 1C 279 +/- 20 236 +/- 9 241 +/- 20 176 +/- 16 -0.018819 -0.089721 -0.034211 0.1710881 0.0882096 DENR density-regulated protein 231 +/- 38 119 +/- 19 238 +/- 27 245 +/- 52 0.136663 -0.031448 0.908312 0.0089864 0.8710639 DFFA DNA fragmentation factor, 45kDa, alpha polypeptide 632 +/- 34 537 +/- 27 694 +/- 27 593 +/- 28 0.028154 -0.054305 -0.031207 0.1962728 0.2976787 DGCR6 DiGeorge syndrome critical region gene 6 979 +/- 27 906 +/- 45 1037 +/- 42 905 +/- 24 0.277388 -0.196474 -0.026268 0.1973073 0.0392253 DHX16 DEAH (Asp-Glu-Ala-His) box polypeptide 16 670 +/- 35 550 +/- 12 568 +/- 33 500 +/- 24 -0.001543 -0.01723 -0.127297 0.0113765 0.3034065 DHX36 DEAH (Asp-Glu-Ala-His) box polypeptide 36 2428 +/- 119 3034 +/- 236 2322 +/- 107 2271 +/- 112 -0.0288 0.0537281 -0.750334 0.0091283 0.7833466 DIP13B DIP13 beta 720 +/- 55 858 +/- 45 786 +/- 23 921 +/- 33 0.241895 0.0820758 0.010297 0.1216435 0.2661512 DIP2C DIP2 disco-interacting protein 2 homolog C (Drosophila) 336 +/- 30 418 +/- 15 445 +/- 23 481 +/- 54 0.02934 0.0454459 0.56088 0.0322439 0.2303166 DKFZP779 PRO0845 1349 +/- 59 1055 +/- 62 1202 +/- 31 955 +/- 46 -0.002435 -0.006254 -0.002851 0.1175901 0.0847625 DLEU8 deleted in lymphocytic leukemia 8 330 +/- 44 467 +/- 42 228 +/- 18 277 +/- 20 -0.002854 0.0495718 0.100475 0.0142841 0.0960935 DLG3 discs, large homolog 3 (neuroendocrine-dlg, Drosophila) 3182 +/- 238 2326 +/- 174 2460 +/- 135 2233 +/- 59 -0.069466 -0.017401 -0.167811 0.0156181 0.4314197 DLGAP4 discs, large (Drosophila) homolog-associated protein 4 5343 +/- 423 4150 +/- 339 3362 +/- 229 2932 +/- 190 -0.000264 -0.053687 -0.182775 0.0176151 0.2355429 DMRTA2 DMRT-like family A2 171 +/- 21 95 +/- 12 262 +/- 37 117 +/- 11 0.057533 -0.012484 -0.009962 0.0533963 0.0209679 DMRTC1 DMRT-like family C1 490 +/- 23 258 +/- 25 324 +/- 18 327 +/- 38 -0.19937 -4.48E-05 0.951774 0.0086286 0.75672 DMTF1 cyclin D binding myb-like transcription factor 1 2324 +/- 160 2497 +/- 93 2237 +/- 55 2007 +/- 60 -0.025523 0.3743629 -0.021051 0.1431864 0.1538993 DNAJA5 DnaJ homology subfamily A member 5 145 +/- 11 219 +/- 23 120 +/- 15 134 +/- 16 -0.006805 0.0223379 0.546184 0.0218671 0.9701908 DNAJB4 DnaJ (Hsp40) homolog, subfamily B, member 4 249 +/- 44 273 +/- 40 286 +/- 16 415 +/- 45 0.048566 0.6888496 0.042426 0.6548772 0.1443555 DNAJB5 DnaJ (Hsp40) homolog, subfamily B, member 5 1205 +/- 78 888 +/- 41 1163 +/- 41 974 +/- 62 0.797996 -0.007385 -0.038186 0.0029897 0.0444014 DNAJB6 DnaJ (Hsp40) homolog, subfamily B, member 6 3673 +/- 317 2730 +/- 233 3649 +/- 170 3344 +/- 450 0.519018 -0.039542 -0.553794 0.0553449 0.4061838 DNAJC7 DnaJ (Hsp40) homolog, subfamily C, member 7 2338 +/- 63 1862 +/- 122 2348 +/- 84 2059 +/- 107 0.18815 -0.009345 -0.065691 0.0345218 0.3819805 DNAPTP6 DNA polymerase-transactivated protein 6 3467 +/- 109 3025 +/- 90 3104 +/- 157 3123 +/- 114 -0.13326 -0.011511 0.925008 0.0015741 0.6415894 DNM2 dynamin 2 623 +/- 66 744 +/- 63 541 +/- 14 626 +/- 29 -0.040153 0.2143312 0.04064 0.1455557 0.0790072 DNPEP aspartyl aminopeptidase 711 +/- 27 636 +/- 18 663 +/- 42 616 +/- 40 -0.34376 -0.044533 -0.436049 0.0926153 0.3497293

S1- alphabetical list of aging-related genes.xls Page 8 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG DOC2B double C2-like domains, beta 258 +/- 27 166 +/- 20 253 +/- 19 182 +/- 20 0.994989 -0.023575 -0.030196 0.0071815 0.0204606 DOCK4 dedicator of cytokinesis 4 83 +/- 24 256 +/- 47 145 +/- 31 379 +/- 38 0.004588 0.0118613 0.001341 0.0689508 0.102621 DOK4 docking protein 4 476 +/- 29 384 +/- 21 433 +/- 40 391 +/- 20 -0.35646 -0.031212 -0.376148 0.0008963 0.4148033 DOK6 docking protein 6 1184 +/- 243 182 +/- 52 1228 +/- 38 204 +/- 68 0.238116 -0.00839 -6.71E-06 0.0219247 0.0162108 DPF1 D4, zinc and double PHD fingers family 1 1631 +/- 91 1083 +/- 88 1997 +/- 111 1529 +/- 115 0.001532 -0.001498 -0.017396 0.0084248 0.1077978 DPM2 dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit 870 +/- 18 1041 +/- 46 787 +/- 41 847 +/- 24 -0.004586 0.0119429 0.24205 0.0784903 0.3300363 DRG2 developmentally regulated GTP binding protein 2 573 +/- 52 606 +/- 35 521 +/- 31 660 +/- 42 0.740595 0.6166757 0.031427 0.9007961 0.1741451 DST dystonin 236 +/- 21 370 +/- 38 341 +/- 33 339 +/- 42 0.136939 0.0151058 -0.977158 0.0064604 0.3652233 DTX3L deltex 3-like (Drosophila) 251 +/- 13 312 +/- 18 280 +/- 11 256 +/- 22 -0.422602 0.0240969 -0.357979 0.0931643 0.3178735 DUS3L dihydrouridine synthase 3-like (S. cerevisiae) 283 +/- 16 306 +/- 11 221 +/- 15 291 +/- 20 -0.028388 0.273665 0.024862 0.3718957 0.1447019 DUSP14 dual specificity phosphatase 14 990 +/- 110 838 +/- 108 1439 +/- 43 1252 +/- 51 0.001272 -0.347324 -0.021931 0.3335945 0.1388966 DUSP16 dual specificity phosphatase 16 390 +/- 59 565 +/- 40 382 +/- 19 384 +/- 22 -0.062438 0.0381487 0.953696 0.0353272 0.8840471 DYM dymeclin 885 +/- 42 957 +/- 31 771 +/- 20 925 +/- 49 -0.025964 0.2012926 0.030225 0.5115503 0.0192984 DYNC2H1 dynein, cytoplasmic 2, heavy chain 1 508 +/- 62 728 +/- 72 644 +/- 82 957 +/- 115 0.003354 0.0439453 0.060176 0.2626924 0.3443156 DYNC2LI1 dynein, cytoplasmic 2, light intermediate chain 1 663 +/- 56 564 +/- 36 662 +/- 33 520 +/- 17 -0.601184 -0.174754 -0.005517 0.3367994 0.0729974 DYRK2 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 831 +/- 140 590 +/- 62 593 +/- 37 489 +/- 19 -0.081726 -0.160623 -0.040427 0.1266689 0.1330754 DZIP1 DAZ interacting protein 1 1130 +/- 99 875 +/- 66 1406 +/- 101 904 +/- 129 0.050224 -0.060364 -0.015726 0.1075366 0.0672343 DZIP1L DAZ interacting protein 1-like 133 +/- 14 134 +/- 14 213 +/- 16 147 +/- 7 0.00727 0.975474 -0.006561 0.9479433 0.0103985 E2F3 E2F transcription factor 3 813 +/- 106 594 +/- 60 697 +/- 22 526 +/- 21 -0.190626 -0.111154 -0.000359 0.0884421 0.0277415 EBF2 early B-cell factor 2 119 +/- 5 103 +/- 3 119 +/- 8 91 +/- 19 -0.58918 -0.028084 -0.241927 0.0820613 0.4058296 ECD ecdysoneless homolog (Drosophila) 625 +/- 49 805 +/- 64 622 +/- 29 839 +/- 52 0.495522 0.0518555 0.009974 0.0080625 0.0248749 ECHDC1 enoyl Coenzyme A hydratase domain containing 1 1796 +/- 60 1599 +/- 72 1651 +/- 62 1404 +/- 53 -0.003041 -0.061427 -0.013714 0.6316257 0.1433039 ECRG4 esophageal cancer related gene 4 protein 529 +/- 11 661 +/- 53 803 +/- 58 778 +/- 126 0.00793 0.0538984 -0.863526 0.0081832 0.3276857 EDD1 E3 ubiquitin protein ligase, HECT domain containing, 1 880 +/- 28 980 +/- 21 1000 +/- 50 955 +/- 46 0.269351 0.0177322 -0.513782 0.0471093 0.4856002 EDF1 endothelial differentiation-related factor 1 2924 +/- 89 3195 +/- 140 2664 +/- 103 3111 +/- 63 -0.081532 0.1393159 0.006026 0.0524779 0.0028085 EDG1 endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 2404 +/- 126 2634 +/- 244 2878 +/- 139 2330 +/- 117 0.202934 0.4293595 -0.014497 0.4495916 0.0176538 EDG2 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 5091 +/- 735 6481 +/- 945 2992 +/- 289 3972 +/- 198 -0.006517 0.2741158 0.022002 0.0528127 0.027661 EDG4 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 4 173 +/- 16 166 +/- 12 211 +/- 12 168 +/- 8 0.169011 -0.730366 -0.014332 0.8755287 0.0104144 EDG7 Endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7 906 +/- 85 1108 +/- 56 920 +/- 115 1256 +/- 85 0.270578 0.0789611 0.044672 0.0544164 0.1609179 EEF1A1 eukaryotic translation elongation factor 1 alpha 1 11705 +/- 649 13512 +/- 606 11114 +/- 370 12852 +/- 588 -0.195276 0.0694277 0.041287 0.3456743 0.4914076 EEF1A2 eukaryotic translation elongation factor 1 alpha 2 12229 +/- 451 10754 +/- 445 11925 +/- 233 11098 +/- 267 0.93132 -0.042274 -0.046167 0.0143564 0.3813578 EFCAB2 EF-hand calcium binding domain 2 1046 +/- 47 1331 +/- 56 1121 +/- 95 1272 +/- 82 0.705551 0.0031117 0.260353 0.0092354 0.7531719 EFEMP1 EGF-containing fibulin-like extracellular matrix protein 1 5116 +/- 149 5852 +/- 332 4920 +/- 274 5811 +/- 259 -0.968883 0.0863104 0.042475 0.0222773 0.1553735 EFHA2 EF-hand domain family, member A2 3623 +/- 194 3050 +/- 248 4234 +/- 119 3864 +/- 96 0.006972 -0.100358 -0.038748 0.0544414 0.2160947 EFHC2 EF-hand domain (C-terminal) containing 2 234 +/- 19 266 +/- 17 174 +/- 10 237 +/- 7 -0.003752 0.235678 0.000755 0.3789393 0.0642173 EFNB2 ephrin-B2 1795 +/- 111 1406 +/- 118 910 +/- 113 742 +/- 59 -4.69E-06 -0.03715 -0.223194 0.0464358 0.3631596 EGLN1 egl nine homolog 1 (C. elegans) 599 +/- 67 426 +/- 24 799 +/- 81 561 +/- 31 0.013278 -0.050058 -0.031492 0.0742366 0.0520918 EGLN2 egl nine homolog 2 (C. elegans) 198 +/- 34 157 +/- 24 231 +/- 12 268 +/- 10 0.022033 -0.34357 0.04326 0.445131 0.4164312 EI24 etoposide induced 2.4 mRNA 1967 +/- 197 2517 +/- 46 2093 +/- 202 2274 +/- 91 -0.648435 0.0374714 0.441747 0.0391908 0.2101078 EID-3 EID-2-like inhibitor of differentiation-3 1170 +/- 82 1234 +/- 63 1297 +/- 45 1499 +/- 44 0.003591 0.5512022 0.010926 0.8176413 0.0879027 EIF1AX eukaryotic translation initiation factor 1A, X-linked 237 +/- 22 1333 +/- 96 475 +/- 143 1352 +/- 99 0.415428 4.71E-05 0.00082 0.0002253 0.0525047 EIF2AK4 eukaryotic translation initiation factor 2 alpha kinase 4 413 +/- 20 484 +/- 18 343 +/- 27 326 +/- 8 -0.000295 0.0263662 -0.570408 0.0087158 0.5093347 EIF3S7 eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa 2662 +/- 54 2791 +/- 67 2401 +/- 59 2691 +/- 31 -0.004037 0.1657818 0.002838 0.2860517 0.0059068 EIF3S9 eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa 3204 +/- 180 2481 +/- 170 2961 +/- 100 2491 +/- 175 -0.387142 -0.01553 -0.055559 0.1213205 0.4933105

S1- alphabetical list of aging-related genes.xls Page 9 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG EIF4G3 eukaryotic translation initiation factor 4 gamma, 3 3958 +/- 161 4220 +/- 172 3636 +/- 92 4030 +/- 135 -0.153024 0.2921752 0.045357 0.4686162 0.0015409 EIF5A2 eukaryotic translation initiation factor 5A2 834 +/- 58 677 +/- 46 773 +/- 40 626 +/- 24 -0.107501 -0.062435 -0.013845 0.2520963 0.0887465 EIF5B eukaryotic translation initiation factor 5B 746 +/- 19 848 +/- 36 734 +/- 20 791 +/- 15 -0.252486 0.0371269 0.052223 0.0020477 0.0552854 ELAC1 elaC homolog 1 (E. coli) 257 +/- 16 328 +/- 22 295 +/- 19 351 +/- 12 0.005793 0.0277609 0.037583 0.0035732 0.0142275 ELAVL3 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) 189 +/- 16 110 +/- 15 287 +/- 18 170 +/- 21 0.001759 -0.005034 -0.002436 0.013616 0.031892 ELF3 E74-like factor 3 (ets domain transcription factor, epithelial-specific ) 129 +/- 10 68 +/- 11 136 +/- 10 86 +/- 15 0.139917 -0.002287 -0.022561 0.0009517 0.0674202 ELK3 ELK3, ETS-domain protein (SRF accessory protein 2) 355 +/- 29 436 +/- 19 346 +/- 23 388 +/- 29 -0.207108 0.0421148 0.281535 0.0275506 0.4790472 ELOVL4 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4 1955 +/- 115 1623 +/- 79 1858 +/- 101 1621 +/- 69 -0.630383 -0.04206 -0.086909 0.0266101 0.5416739 ELTD1 EGF, latrophilin and seven transmembrane domain containing 1 216 +/- 15 319 +/- 20 190 +/- 19 228 +/- 17 -0.026701 0.0022995 0.16525 0.0005254 0.2394358 EMD emerin (Emery-Dreifuss muscular dystrophy) 883 +/- 36 950 +/- 27 898 +/- 19 978 +/- 23 0.544975 0.1708619 0.026642 0.7273092 0.3835798 EML4 echinoderm microtubule associated protein like 4 276 +/- 12 333 +/- 21 362 +/- 36 401 +/- 18 0.002134 0.0464792 0.35486 0.0068409 0.5067077 EMP2 epithelial membrane protein 2 131 +/- 20 209 +/- 17 138 +/- 17 214 +/- 16 0.498061 0.0133384 0.011268 0.0207786 0.0159111 EMP3 epithelial membrane protein 3 385 +/- 23 473 +/- 25 311 +/- 21 374 +/- 24 -0.001739 0.0295194 0.081156 0.0951945 0.7970266 EMX2 empty spiracles homolog 2 (Drosophila) 860 +/- 116 836 +/- 85 1476 +/- 75 1238 +/- 46 0.000166 -0.874806 -0.027446 0.900048 0.0081431 ENO1 enolase 1, (alpha) 7846 +/- 333 9198 +/- 336 6480 +/- 135 7880 +/- 253 -0.000391 0.0169672 0.00252 0.0783902 0.0568711 EP400 E1A binding protein p400 812 +/- 48 952 +/- 33 925 +/- 77 961 +/- 76 0.115793 0.0389664 0.741597 0.0088177 0.300628 EPB41L5 erythrocyte membrane protein band 4.1 like 5 80 +/- 8 128 +/- 8 94 +/- 11 112 +/- 3 -0.815495 0.002337 0.173636 0.0012424 0.3634233 EPHB6 EPH receptor B6 1193 +/- 220 1171 +/- 59 814 +/- 60 1044 +/- 67 -0.122707 -0.925675 0.032399 0.7972843 0.1634836 EPS15 epidermal growth factor receptor pathway substrate 15 6961 +/- 240 6152 +/- 333 6822 +/- 320 5815 +/- 233 -0.582912 -0.079764 -0.032351 0.5176281 0.1514397 EPS8 epidermal growth factor receptor pathway substrate 8 1375 +/- 55 1537 +/- 28 1326 +/- 75 1257 +/- 47 -0.015856 0.0333534 -0.451632 0.0403725 0.1494764 ERBB2IP erbb2 interacting protein 1656 +/- 204 2474 +/- 294 1821 +/- 122 2048 +/- 58 -0.515613 0.0485273 0.137143 0.0054073 0.2314574 ERGIC3 ERGIC and golgi 3 2303 +/- 107 2433 +/- 73 2054 +/- 51 2319 +/- 42 -0.015545 0.3425289 0.003142 0.8177734 0.1119695 ETF1 eukaryotic translation termination factor 1 243 +/- 4 206 +/- 11 313 +/- 29 253 +/- 16 0.004699 -0.015712 -0.113144 0.0736888 0.1713913 ETFA electron-transfer-flavoprotein, alpha polypeptide (glutaric aciduria II) 763 +/- 80 635 +/- 93 926 +/- 45 580 +/- 121 0.182268 -0.323308 -0.043134 0.653015 0.1909163 ETFDH electron-transferring-flavoprotein dehydrogenase 382 +/- 17 463 +/- 16 356 +/- 16 395 +/- 22 -0.011825 0.0066056 0.190975 0.1096217 0.4593126 EXOC7 exocyst complex component 7 228 +/- 32 391 +/- 33 285 +/- 12 351 +/- 36 0.513132 0.004969 0.144338 0.0761158 0.2113179 EXOSC10 exosome component 10 957 +/- 62 1170 +/- 42 800 +/- 35 895 +/- 36 -0.000661 0.0202287 0.092599 0.0296892 0.1904323 EXOSC5 exosome component 5 648 +/- 33 544 +/- 16 532 +/- 31 483 +/- 14 -0.008 -0.025109 -0.20146 0.0251747 0.4908932 FA2H fatty acid 2-hydroxylase 1088 +/- 162 1680 +/- 349 622 +/- 81 901 +/- 73 -0.012014 0.1668608 0.030927 0.0274475 0.011596 FADD Fas (TNFRSF6)-associated via death domain 417 +/- 17 322 +/- 14 411 +/- 35 337 +/- 8 0.802694 -0.001535 -0.090736 0.0364948 0.3206309 FADS3 fatty acid desaturase 3 1186 +/- 74 995 +/- 86 1240 +/- 69 944 +/- 62 0.720485 -0.123483 -0.010979 0.1789204 0.0811987 FAIM Fas apoptotic inhibitory molecule 608 +/- 41 920 +/- 63 711 +/- 51 974 +/- 37 0.112615 0.0027375 0.00246 0.0032731 0.0292477 FANCM Fanconi anemia, complementation group M 187 +/- 20 162 +/- 14 232 +/- 6 158 +/- 16 0.195764 -0.347211 -0.007508 0.511436 0.0197887 FARP1 FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) 387 +/- 68 1140 +/- 179 336 +/- 59 910 +/- 114 -0.005662 0.0066696 0.004169 0.1398067 0.0912823 FARP2 FERM, RhoGEF and pleckstrin domain protein 2 207 +/- 32 348 +/- 65 210 +/- 30 315 +/- 16 -0.555578 0.0929471 0.015546 0.0052674 0.0183646 FASTK Fas-activated serine/threonine kinase 625 +/- 37 797 +/- 40 549 +/- 14 706 +/- 35 -0.014604 0.010554 0.0082 0.048923 0.0043773 FAT3 FAT tumor suppressor homolog 3 (Drosophila) 802 +/- 78 532 +/- 31 691 +/- 50 625 +/- 62 -0.85823 -0.016377 -0.430341 0.0445128 0.8837028 FAT4 FAT tumor suppressor homolog 4 (Drosophila) 293 +/- 14 306 +/- 33 708 +/- 23 485 +/- 48 0.000342 0.734089 -0.005929 0.523441 0.1234583 FBLN1 fibulin 1 358 +/- 58 415 +/- 18 289 +/- 15 381 +/- 10 -0.114801 0.3817661 0.000674 0.2249587 0.0110259 FBP1 fructose-1,6-bisphosphatase 1 89 +/- 10 125 +/- 32 81 +/- 8 136 +/- 13 0.759081 0.3232018 0.009196 0.8116737 0.0144975 FBXL12 F-box and leucine-rich repeat protein 12 183 +/- 11 198 +/- 17 225 +/- 11 184 +/- 12 0.303886 0.4935119 -0.040901 0.2257533 0.1786531 FBXL14 F-box and leucine-rich repeat protein 14 1117 +/- 114 749 +/- 52 1272 +/- 40 979 +/- 38 0.01286 -0.022121 -0.000484 0.07579 0.0310178 FBXL3 F-box and leucine-rich repeat protein 3 2062 +/- 73 1887 +/- 100 2305 +/- 53 1970 +/- 46 0.072848 -0.188586 -0.000995 0.1538691 0.0055467 FBXL5 F-box and leucine-rich repeat protein 5 281 +/- 17 233 +/- 9 307 +/- 13 264 +/- 18 0.037003 -0.036268 -0.084253 0.1025076 0.2376194

S1- alphabetical list of aging-related genes.xls Page 10 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG FBXO17 F-box protein 17 244 +/- 20 351 +/- 30 356 +/- 58 418 +/- 44 0.032456 0.0166647 0.414235 0.1175588 0.2090408 FBXO22 F-box protein 22 180 +/- 15 116 +/- 11 204 +/- 17 146 +/- 19 0.02693 -0.007922 -0.047841 0.0438841 0.0742148 FBXO38 F-box protein 38 480 +/- 20 548 +/- 23 535 +/- 44 505 +/- 38 0.848991 0.0461007 -0.619412 0.307002 0.4390292 FCER1G Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide 283 +/- 30 393 +/- 40 195 +/- 12 277 +/- 15 -0.002161 0.0544574 0.002516 0.0320568 0.0074183 FCGR3B Fc fragment of IgG, low affinity IIIb, receptor (CD16b) 158 +/- 14 222 +/- 21 125 +/- 11 167 +/- 20 -0.001601 0.0307221 0.112317 0.0083523 0.0039974 FCHO2 FCH domain only 2 843 +/- 52 1043 +/- 48 843 +/- 51 914 +/- 52 -0.201113 0.0177434 0.356726 0.0057916 0.2676989 FCHSD2 FCH and double SH3 domains 2 602 +/- 50 541 +/- 45 432 +/- 23 355 +/- 19 -0.000261 -0.3862 -0.031367 0.7664457 0.1745995 FCN3 ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen) 773 +/- 82 612 +/- 38 616 +/- 53 473 +/- 26 -0.007798 -0.119355 -0.044076 0.2732374 0.2516669 FECH ferrochelatase (protoporphyria) 822 +/- 52 1099 +/- 86 820 +/- 77 1136 +/- 119 0.772196 0.0239491 0.059793 0.0826612 0.0770816 FEN1 flap structure-specific endonuclease 1 189 +/- 9 162 +/- 6 178 +/- 12 185 +/- 19 0.793427 -0.033609 0.754614 0.0065744 0.7169363 FEZ1 fasciculation and elongation protein zeta 1 (zygin I) 8114 +/- 604 9755 +/- 837 6969 +/- 218 8397 +/- 232 -0.032367 0.1460565 0.001635 0.0511895 0.0020014 FHOD3 formin homology 2 domain containing 3 907 +/- 38 718 +/- 69 579 +/- 57 434 +/- 53 -1.2E-05 -0.043636 -0.096171 0.0103365 0.3282478 FKBP3 FK506 binding protein 3, 25kDa 2550 +/- 62 2815 +/- 70 2687 +/- 106 2837 +/- 134 0.260528 0.0182882 0.403423 0.0034066 0.3297541 FKRP fukutin related protein 1114 +/- 40 971 +/- 30 1164 +/- 29 1050 +/- 21 0.003275 -0.018583 -0.010989 0.0373924 0.1118994 FLCN folliculin 85 +/- 4 102 +/- 4 108 +/- 14 107 +/- 6 0.186896 0.0192329 -0.937967 0.0820393 0.3848393 FLII flightless I homolog (Drosophila) 1073 +/- 71 1217 +/- 48 871 +/- 23 1050 +/- 35 -0.002118 0.1284841 0.003355 0.1624965 0.0318487 FLJ00038 CXYorf1-related protein 477 +/- 9 660 +/- 29 559 +/- 32 584 +/- 37 0.842738 0.0010179 0.630895 0.0045185 0.6422751 FLJ25530 hepatocyte cell adhesion molecule 4362 +/- 359 5227 +/- 460 3822 +/- 130 4496 +/- 91 -0.014625 0.171006 0.002411 0.064663 0.003096 FLJ30092 AF-1 specific protein phosphatase 3538 +/- 114 3147 +/- 187 3387 +/- 111 2870 +/- 54 -0.170234 -0.110711 -0.003969 0.0512134 0.0776709 FLJ35348 FLJ35348 1340 +/- 120 959 +/- 56 1239 +/- 60 1161 +/- 20 0.746881 -0.023204 -0.260509 0.0089147 0.4034201 FLJ37035 FLJ37035 protein 344 +/- 33 221 +/- 20 323 +/- 33 207 +/- 33 -0.550993 -0.012535 -0.035518 0.0414483 0.1060541 FLJ39237 FLJ39237 protein 202 +/- 20 175 +/- 23 202 +/- 16 152 +/- 11 -0.531022 -0.390375 -0.028344 0.8533485 0.3503263 FLJ41131 FLJ41131 protein 131 +/- 16 159 +/- 16 101 +/- 4 144 +/- 10 -0.203452 0.2471598 0.010712 0.3678931 0.0016689 FLJ42875 FLJ42875 protein 320 +/- 16 256 +/- 17 326 +/- 29 256 +/- 19 0.839771 -0.01936 -0.079189 0.3165387 0.1547585 FLNC filamin C, gamma (actin binding protein 280) 423 +/- 64 485 +/- 58 196 +/- 21 278 +/- 15 -0.000385 0.4850993 0.012306 0.4644705 0.0496698 FLT3 fms-related tyrosine kinase 3 200 +/- 23 262 +/- 27 110 +/- 9 165 +/- 6 -0.000328 0.1216677 0.000867 0.1610886 0.0425169 FN3KRP fructosamine-3-kinase-related protein 2327 +/- 186 2932 +/- 222 2878 +/- 211 3527 +/- 36 0.006278 0.0643649 0.027114 0.2476558 0.0954689 FNDC5 fibronectin type III domain containing 5 519 +/- 27 422 +/- 25 470 +/- 26 433 +/- 42 -0.627101 -0.024752 -0.489434 0.0305327 0.2422757 FOXK1 forkhead box K1 1751 +/- 65 1597 +/- 115 1911 +/- 71 1533 +/- 131 0.062205 -0.279784 -0.042521 0.3870735 0.0940586 FOXO1A forkhead box O1A (rhabdomyosarcoma) 720 +/- 44 1108 +/- 81 1000 +/- 116 1225 +/- 114 0.046704 0.0031203 0.199946 0.0662699 0.9658058 FOXO3A Forkhead box O3A 331 +/- 35 512 +/- 68 283 +/- 27 375 +/- 22 -0.005025 0.0486744 0.026861 0.0574577 0.0218273 FOXP1 forkhead box P1 285 +/- 29 214 +/- 23 213 +/- 24 144 +/- 16 -0.006384 -0.086046 -0.043082 0.7429002 0.2205373 FRMD3 FERM domain containing 3 159 +/- 13 124 +/- 13 144 +/- 8 115 +/- 10 -0.244571 -0.090893 -0.04872 0.10747 0.0573653 FRMD4A FERM domain containing 4A 926 +/- 37 796 +/- 36 829 +/- 20 663 +/- 39 -0.002359 -0.030713 -0.008665 0.1317086 0.0397146 FSTL1 follistatin-like 1 1444 +/- 104 1350 +/- 62 956 +/- 42 1093 +/- 20 -0.000477 -0.458445 0.021569 0.493828 0.1436632 FSTL5 follistatin-like 5 790 +/- 173 669 +/- 70 1194 +/- 114 623 +/- 58 0.169852 -0.537903 -0.002692 0.4939755 0.0400866 FTL ferritin, light polypeptide 16238 +/- 1033 18500 +/- 704 14150 +/- 408 17258 +/- 618 -0.026136 0.1045271 0.00385 0.1129855 0.1026607 FTSJ2 FtsJ homolog 2 (E. coli) 498 +/- 18 476 +/- 31 406 +/- 8 463 +/- 17 -0.012851 -0.548167 0.02704 0.1548927 0.031354 FUBP3 far upstream element (FUSE) binding protein 3 1363 +/- 127 1472 +/- 64 1328 +/- 43 1188 +/- 31 -0.129335 0.4660081 -0.026881 0.2734257 0.0251975 FUT1 Fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, H blood group) 147 +/- 13 121 +/- 9 139 +/- 12 91 +/- 13 -0.122949 -0.136086 -0.025098 0.0477271 0.0464862 FXYD7 FXYD domain containing ion transport regulator 7 3435 +/- 178 2940 +/- 109 4078 +/- 119 3624 +/- 147 0.000845 -0.044077 -0.042429 0.0048882 0.4833482 FYCO1 FYVE and coiled-coil domain containing 1 428 +/- 37 875 +/- 195 355 +/- 20 531 +/- 43 -0.074771 0.0705324 0.01151 0.1195917 0.1176505 FYN FYN oncogene related to SRC, FGR, YES 243 +/- 42 94 +/- 25 298 +/- 27 175 +/- 27 0.019308 -0.014817 -0.010902 0.0085926 0.0075934 G3BP Ras-GTPase-activating protein SH3-domain-binding protein 1810 +/- 99 1689 +/- 83 1957 +/- 54 1678 +/- 67 0.179365 -0.372115 -0.011908 0.8615342 0.1403157

S1- alphabetical list of aging-related genes.xls Page 11 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG GABARAP GABA(A) receptor-associated protein 11702 +/- 341 11960 +/- 190 10752 +/- 157 11400 +/- 130 -0.00177 0.5282196 0.011299 0.5584819 0.0638276 GABBR2 gamma-aminobutyric acid (GABA) B receptor, 2 1834 +/- 197 1551 +/- 193 2778 +/- 78 2396 +/- 114 0.000164 -0.329709 -0.026833 0.295949 0.324515 GABPA GA binding protein transcription factor, alpha subunit 60kDa 387 +/- 45 419 +/- 22 469 +/- 23 342 +/- 6 0.820254 0.5392529 -0.001907 0.438208 0.0268951 GABRG3 gamma-aminobutyric acid (GABA) A receptor, gamma 3 83 +/- 11 74 +/- 14 144 +/- 6 100 +/- 6 0.000909 -0.596888 -0.000761 0.4350859 0.0006531 GALNTL2 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 448 +/- 45 296 +/- 38 272 +/- 18 235 +/- 32 -0.010103 -0.026776 -0.343099 0.0897514 0.3666745 GAMT guanidinoacetate N-methyltransferase 1022 +/- 43 1199 +/- 29 1147 +/- 61 1359 +/- 156 0.043166 0.009401 0.261073 0.0097846 0.1781006 GANC glucosidase, alpha; neutral C 451 +/- 60 883 +/- 120 322 +/- 16 530 +/- 33 -0.013584 0.0135516 0.001369 0.0051561 0.0059706 GAPVD1 GTPase activating protein and VPS9 domains 1 1036 +/- 57 938 +/- 97 956 +/- 26 845 +/- 31 -0.085428 -0.405966 -0.023563 0.8008104 0.4174119 GARNL1 GTPase activating Rap/RanGAP domain-like 1 683 +/- 64 626 +/- 46 579 +/- 30 462 +/- 37 -0.012651 -0.48589 -0.040099 0.9233998 0.0687345 GAS7 growth arrest-specific 7 9122 +/- 492 10812 +/- 463 7341 +/- 461 8180 +/- 533 -0.000193 0.0313393 0.265978 0.1668645 0.1972682 GCAT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) 417 +/- 20 365 +/- 15 436 +/- 24 357 +/- 20 0.377023 -0.071902 -0.031789 0.0385055 0.0279241 GCDH glutaryl-Coenzyme A dehydrogenase 441 +/- 27 371 +/- 17 418 +/- 24 317 +/- 19 -0.043161 -0.058952 -0.009162 0.0303135 0.0002663 GCHFR GTP cyclohydrolase I feedback regulator 417 +/- 27 263 +/- 11 403 +/- 32 315 +/- 18 0.471925 -0.001233 -0.044122 0.0036029 0.2626674 GCLM glutamate-cysteine ligase, modifier subunit 771 +/- 36 943 +/- 26 851 +/- 27 1048 +/- 44 0.014231 0.0036647 0.007251 0.0229726 0.0130043 GEMIN6 gem (nuclear organelle) associated protein 6 120 +/- 23 102 +/- 10 117 +/- 13 77 +/- 3 -0.177933 -0.493262 -0.024097 0.2569431 0.0779739 GFM1 G elongation factor, mitochondrial 1 175 +/- 15 233 +/- 22 216 +/- 6 265 +/- 11 0.043576 0.0637293 0.006752 0.1726557 0.0006379 GFM2 G elongation factor, mitochondrial 2 1138 +/- 34 958 +/- 37 1177 +/- 64 993 +/- 71 0.363163 -0.005135 -0.087734 0.0450763 0.2241799 GGH gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) 1157 +/- 71 923 +/- 51 1159 +/- 47 1032 +/- 52 0.21465 -0.024736 -0.103654 0.0306848 0.2273713 GGPS1 Geranylgeranyl diphosphate synthase 1 96 +/- 10 181 +/- 17 152 +/- 9 155 +/- 10 0.121289 0.0025016 0.803336 0.00128 0.7078459 GGTL3 gamma-glutamyltransferase-like 3 2529 +/- 133 2114 +/- 121 2837 +/- 156 2630 +/- 103 0.001707 -0.044208 -0.298117 0.1027986 0.4930832 GGTLA4 gamma-glutamyltransferase-like activity 4 155 +/- 2 188 +/- 13 139 +/- 10 162 +/- 8 -0.043682 0.0487262 0.12909 0.0644891 0.1799802 GINS1 GINS complex subunit 1 (Psf1 homolog) 2077 +/- 122 2623 +/- 181 2094 +/- 32 2596 +/- 160 -0.513677 0.0343205 0.033856 0.3674072 0.3453354 GIT2 G protein-coupled receptor kinase interactor 2 2774 +/- 76 3131 +/- 101 2423 +/- 103 2775 +/- 131 -0.004492 0.0196676 0.067079 0.1746952 0.0318407 GJA1 gap junction protein, alpha 1, 43kDa (connexin 43) 11658 +/- 328 13350 +/- 420 12800 +/- 751 13085 +/- 225 0.327344 0.0106693 0.728814 0.0135463 0.8528205 GJA12 gap junction protein, alpha 12, 47kDa 683 +/- 86 921 +/- 117 493 +/- 44 660 +/- 35 -0.026775 0.1357396 0.015548 0.0902397 0.1246776 GK glycerol kinase 118 +/- 7 90 +/- 9 112 +/- 5 102 +/- 8 0.620331 -0.034465 -0.312875 0.1003719 0.1468505 GLA galactosidase, alpha 400 +/- 19 475 +/- 12 357 +/- 11 391 +/- 12 -0.005319 0.0101419 0.061628 0.0135964 0.3486946 GLDN gliomedin 316 +/- 58 508 +/- 25 308 +/- 25 409 +/- 21 -0.142476 0.0195794 0.013554 0.0270417 0.1303341 GLG1 golgi apparatus protein 1 4000 +/- 666 6938 +/- 706 3906 +/- 536 6715 +/- 578 -0.664447 0.0127739 0.006455 0.0200505 0.0060216 GLI2 GLI-Kruppel family member GLI2 129 +/- 11 140 +/- 14 176 +/- 10 134 +/- 12 0.036059 0.529673 -0.024784 0.3778247 0.0012399 GMFG glia maturation factor, gamma 412 +/- 31 565 +/- 21 316 +/- 21 420 +/- 14 -1.16E-05 0.0027447 0.002888 0.0042369 0.048963 GMPS guanine monphosphate synthetase 570 +/- 45 619 +/- 27 438 +/- 20 530 +/- 21 -0.001525 0.3776871 0.011127 0.4369728 0.0406228 GNB2L1 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 8227 +/- 314 9584 +/- 311 7947 +/- 439 9269 +/- 228 -0.177507 0.0118215 0.030346 0.0378873 0.0959707 GNB5 guanine nucleotide binding protein (G protein), beta 5 2978 +/- 211 2392 +/- 140 2607 +/- 211 2224 +/- 172 -0.12632 -0.046541 -0.192619 0.0519976 0.4909399 GNG2 guanine nucleotide binding protein (G protein), gamma 2 7405 +/- 371 5485 +/- 393 7908 +/- 317 6914 +/- 174 0.032401 -0.005281 -0.026402 0.0148569 0.0383324 GNL2 guanine nucleotide binding protein-like 2 (nucleolar) 347 +/- 23 320 +/- 17 374 +/- 23 301 +/- 18 0.674212 -0.378445 -0.03345 0.8352457 0.1096222 GNMT glycine N-methyltransferase 171 +/- 11 138 +/- 16 174 +/- 20 111 +/- 11 -0.347997 -0.126863 -0.024455 0.2559391 0.028209 GNPTG N-acetylglucosamine-1-phosphate transferase, gamma subunit 1259 +/- 45 970 +/- 51 1269 +/- 41 946 +/- 36 -0.68594 -0.001691 -0.000242 0.0382462 0.0161032 GOLGA2 golgi autoantigen, golgin subfamily a, 2 80 +/- 6 112 +/- 6 118 +/- 19 140 +/- 9 0.009563 0.004714 0.328757 0.0191052 0.5915585 GOLGA4 golgi autoantigen, golgin subfamily a, 4 158 +/- 20 223 +/- 19 236 +/- 13 257 +/- 20 0.001219 0.0401902 0.414473 0.0194827 0.1549389 GOLT1B golgi transport 1 homolog B (S. cerevisiae) 971 +/- 73 734 +/- 56 960 +/- 48 651 +/- 52 -0.377981 -0.029643 -0.002022 0.0378344 0.0055392 GON4L gon-4-like (C.elegans) 260 +/- 13 344 +/- 16 310 +/- 44 351 +/- 20 0.185793 0.0023392 0.428239 0.0011843 0.7783808 GOPC golgi associated PDZ and coiled-coil motif containing 462 +/- 34 622 +/- 43 560 +/- 32 730 +/- 26 0.005407 0.0158442 0.002573 0.0673328 0.0086109 GORASP2 golgi reassembly stacking protein 2, 55kDa 1888 +/- 69 1700 +/- 21 1893 +/- 58 1861 +/- 71 0.122017 -0.040739 -0.737723 0.1605595 0.6351235

S1- alphabetical list of aging-related genes.xls Page 12 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG GPATC1 G patch domain containing 1 206 +/- 10 251 +/- 12 231 +/- 21 230 +/- 9 0.570262 0.0168554 -0.9913 0.0059077 0.9608456 GPATC2 G patch domain containing 2 364 +/- 9 306 +/- 7 390 +/- 22 307 +/- 16 0.151765 -0.000615 -0.014568 0.0151537 0.0039916 GPC4 glypican 4 206 +/- 17 250 +/- 35 346 +/- 28 436 +/- 14 0.000173 0.2887487 0.023212 0.6553604 0.2101967 GPHN Gephyrin 468 +/- 36 658 +/- 46 703 +/- 53 656 +/- 40 0.069208 0.0093398 -0.495339 0.070879 0.2864199 GPKOW G patch domain and KOW motifs 740 +/- 27 793 +/- 31 690 +/- 27 787 +/- 32 -0.184624 0.2349 0.048299 0.302033 0.022967 GPM6A glycoprotein M6A 20299 +/- 453 16530 +/- 744 18774 +/- 263 16875 +/- 764 -0.143631 -0.002342 -0.06605 0.0243419 0.3252601 GPM6B glycoprotein M6B 5149 +/- 133 6879 +/- 254 6195 +/- 386 7340 +/- 323 0.026838 0.0003929 0.049273 0.0074918 0.472334 GPR135 G protein-coupled receptor 135 154 +/- 8 126 +/- 19 203 +/- 9 163 +/- 8 0.000126 -0.21993 -0.012068 0.5096649 0.0210926 GPR153 G protein-coupled receptor 153 395 +/- 32 371 +/- 45 374 +/- 8 300 +/- 22 -0.293169 -0.679135 -0.024123 0.9214764 0.148717 GPR155 G protein-coupled receptor 155 3986 +/- 374 3280 +/- 279 5230 +/- 184 4681 +/- 151 3.3E-05 -0.164043 -0.046513 0.3330083 0.2508683 GPR68 G protein-coupled receptor 68 143 +/- 27 149 +/- 7 197 +/- 12 151 +/- 16 0.193182 0.8448669 -0.046931 0.6715362 0.0022399 GPR89A G protein-coupled receptor 89A 470 +/- 16 578 +/- 23 474 +/- 20 537 +/- 29 -0.56843 0.0043371 0.112575 0.0133367 0.3816914 GPRC5B G protein-coupled receptor, family C, group 5, member B 2685 +/- 255 3426 +/- 164 2738 +/- 97 2936 +/- 111 -0.239552 0.0386148 0.214412 0.0495925 0.5363138 GPSN2 glycoprotein, synaptic 2 2089 +/- 57 2355 +/- 82 2194 +/- 58 2191 +/- 141 -0.996177 0.0263652 -0.98268 0.0117104 0.5629816 GRHPR glyoxylate reductase/hydroxypyruvate reductase 1359 +/- 124 1733 +/- 104 1291 +/- 74 1730 +/- 83 -0.641964 0.0441827 0.003679 0.2286539 0.0686579 GRIK1 glutamate receptor, ionotropic, kainate 1 107 +/- 11 355 +/- 42 65 +/- 9 194 +/- 15 -0.005795 0.0015561 0.000176 0.0077755 0.005943 GRINA glutamate receptor, ionotropic, N-methyl D-asparate-associated protein 1 (glutamate binding 5549 +/- 298 4625 +/- 234 6955 +/- 300 6870 +/- 234 2.45E-05 -0.036044 -0.828973 0.0232317 0.6928194 GRLF1 glucocorticoid receptor DNA binding factor 1 2246 +/- 57 1916 +/- 112 2476 +/- 123 2257 +/- 73 0.029725 -0.031675 -0.162091 0.0059261 0.5659028 GRM5 Glutamate receptor, metabotropic 5 4099 +/- 219 3135 +/- 171 3523 +/- 175 2644 +/- 98 -0.054363 -0.006511 -0.00259 0.0064815 0.0858297 GRSF1 G-rich RNA sequence binding factor 1 2254 +/- 87 1851 +/- 104 2017 +/- 89 2006 +/- 155 -0.596221 -0.014165 -0.955008 0.1787168 0.278694 GRTP1 Growth hormone regulated TBC protein 1 446 +/- 50 299 +/- 12 412 +/- 52 272 +/- 16 -0.178175 -0.030153 -0.042958 0.0352107 0.1037726 GSK3A glycogen synthase kinase 3 alpha 4199 +/- 213 3357 +/- 277 4155 +/- 242 3593 +/- 133 0.784863 -0.038139 -0.078496 0.0164546 0.0481041 GSN gelsolin (amyloidosis, Finnish type) 4180 +/- 541 5269 +/- 560 2845 +/- 206 3577 +/- 214 -0.001124 0.1927344 0.03637 0.2336001 0.1320149 GSTT1 Glutathione S-transferase theta 1 180 +/- 17 259 +/- 19 171 +/- 15 240 +/- 13 -0.697357 0.0126606 0.00744 0.0033555 0.0462028 GTF2H2 general transcription factor IIH, polypeptide 2, 44kDa 104 +/- 9 178 +/- 26 133 +/- 21 150 +/- 15 0.777899 0.0334695 0.540628 0.0253704 0.8989462 GTF2H5 general transcription factor IIH, polypeptide 5 1686 +/- 106 1502 +/- 113 1400 +/- 61 1199 +/- 50 -0.002828 -0.260413 -0.030987 0.2897615 0.0226314 GTF2IRD2 GTF2I repeat domain containing 2 310 +/- 18 224 +/- 27 365 +/- 33 210 +/- 55 0.339862 -0.028055 -0.04721 0.4826959 0.3391896 GTF3C4 General transcription factor IIIC, polypeptide 4, 90kDa 1590 +/- 79 1380 +/- 69 1484 +/- 45 1344 +/- 39 -0.361086 -0.072365 -0.043751 0.0805172 0.1480084 GUF1 GUF1 GTPase homolog (S. cerevisiae) 391 +/- 29 526 +/- 32 365 +/- 39 430 +/- 27 -0.017923 0.0116682 0.209153 0.0437322 0.3849475 GUP1 GUP1 glycerol uptake/transporter homolog (S. cerevisiae) 602 +/- 64 1060 +/- 102 467 +/- 55 753 +/- 60 -0.00411 0.0046362 0.006826 0.0049883 0.0567797 H1F0 H1 histone family, member 0 1586 +/- 121 1504 +/- 164 1458 +/- 52 1178 +/- 25 -0.030654 -0.696287 -0.001738 0.4357901 0.0087921 H3F3A H3 histone, family 3A 1040 +/- 71 1128 +/- 59 1218 +/- 63 874 +/- 46 -0.801652 0.3635832 -0.001899 0.1794462 0.0132891 H6PD hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) 144 +/- 17 242 +/- 29 154 +/- 17 173 +/- 8 -0.200044 0.0197659 0.354235 0.0445199 0.6634603 HADH2 hydroxyacyl-Coenzyme A dehydrogenase, type II 2567 +/- 64 2333 +/- 98 2257 +/- 48 2004 +/- 54 -4.57E-05 -0.078236 -0.007019 0.1175237 0.0859568 HAGH hydroxyacylglutathione hydrolase 3456 +/- 243 2690 +/- 123 2867 +/- 137 2258 +/- 72 -0.000327 -0.024497 -0.005119 0.0301329 0.0219011 HAGHL hydroxyacylglutathione hydrolase-like 559 +/- 53 491 +/- 35 436 +/- 21 337 +/- 5 -0.000591 -0.315099 -0.004581 0.402573 0.0145572 HAX1 HCLS1 associated protein X-1 2262 +/- 81 1987 +/- 38 2212 +/- 35 1989 +/- 78 -0.668707 -0.017283 -0.042094 0.0329426 0.0178014 HBII-276HGHBII-276 host gene 4992 +/- 293 6134 +/- 326 5486 +/- 284 6432 +/- 380 0.262127 0.0264066 0.082377 0.0798043 0.2160083 HBS1L HBS1-like (S. cerevisiae) 1048 +/- 89 1565 +/- 156 1275 +/- 83 1473 +/- 162 0.545985 0.0206289 0.319037 0.0863452 0.9183126 HDAC11 Histone deacetylase 11 3200 +/- 431 3729 +/- 400 2319 +/- 144 2838 +/- 131 -0.009873 0.3894085 0.025646 0.2581662 0.0703184 HDAC6 histone deacetylase 6 858 +/- 46 1082 +/- 96 763 +/- 14 907 +/- 33 -0.011085 0.0726796 0.008625 0.2408406 0.0768492 HECTD1 HECT domain containing 1 154 +/- 14 131 +/- 13 204 +/- 13 162 +/- 11 0.009086 -0.238273 -0.041532 0.852479 0.2248975 HECTD2 HECT domain containing 2 1579 +/- 126 2196 +/- 109 1410 +/- 84 1984 +/- 71 -0.109324 0.0041351 0.000569 0.0807731 0.0415738 HERC1 hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like 3490 +/- 87 3209 +/- 99 3290 +/- 56 3128 +/- 37 -0.051693 -0.059808 -0.04215 0.0071283 0.1525603

S1- alphabetical list of aging-related genes.xls Page 13 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG HERC2P7 hect domain and RLD 2 pseudogene 7 170 +/- 14 126 +/- 21 225 +/- 17 160 +/- 19 0.014426 -0.114895 -0.033002 0.110836 0.0130911 HERC4 hect domain and RLD 4 725 +/- 37 638 +/- 58 882 +/- 55 667 +/- 35 0.057109 -0.240881 -0.010634 0.3338926 0.1338468 HES1 hairy and enhancer of split 1, (Drosophila) 395 +/- 33 353 +/- 23 461 +/- 26 352 +/- 18 0.113672 -0.327047 -0.008026 0.3528417 0.0281143 HES5 hairy and enhancer of split 5 (Drosophila) 714 +/- 104 558 +/- 31 840 +/- 53 603 +/- 61 0.147906 -0.201518 -0.017729 0.1811541 0.0107066 HEXDC hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing 148 +/- 17 180 +/- 18 173 +/- 20 274 +/- 24 0.002521 0.2261819 0.011704 0.4469197 0.0067908 HEXIM1 hexamethylene bis-acetamide inducible 1 1258 +/- 39 1102 +/- 16 1185 +/- 37 1098 +/- 42 -0.018271 -0.008471 -0.155924 0.0188297 0.4940762 HEY2 hairy/enhancer-of-split related with YRPW motif 2 500 +/- 20 595 +/- 32 557 +/- 20 531 +/- 30 -0.861635 0.0360203 -0.480894 0.0436667 0.3612082 HIGD1B HIG1 domain family, member 1B 225 +/- 13 268 +/- 11 154 +/- 8 203 +/- 12 -2.08E-05 0.0303291 0.009653 0.0324301 0.0572933 HIGD2A HIG1 domain family, member 2A 3641 +/- 182 2920 +/- 183 3456 +/- 163 2935 +/- 93 -0.36263 -0.019089 -0.024691 0.0239717 0.0874323 HIPK2 Homeodomain interacting protein kinase 2 13628 +/- 1528 17700 +/- 1910 8772 +/- 365 10920 +/- 395 -0.001574 0.1284122 0.003367 0.0120334 0.0028744 HKR2 GLI-Kruppel family member HKR2 186 +/- 15 198 +/- 14 187 +/- 10 237 +/- 13 0.28536 0.5834441 0.017131 0.9304687 0.0181515 HLA-DMA major histocompatibility complex, class II, DM alpha 100 +/- 12 213 +/- 26 81 +/- 17 132 +/- 17 -0.03817 0.0051739 0.062295 0.0063115 0.1378359 HLA-DPA1 major histocompatibility complex, class II, DP alpha 1 242 +/- 37 469 +/- 34 224 +/- 35 351 +/- 66 -0.052627 0.0011439 0.139667 0.0230307 0.3222405 HLA-DPB1 major histocompatibility complex, class II, DP beta 1 275 +/- 55 485 +/- 42 153 +/- 53 409 +/- 56 -0.000767 0.0138141 0.009355 0.1031803 0.0248301 HLA-DRA major histocompatibility complex, class II, DR alpha 537 +/- 59 893 +/- 114 350 +/- 59 668 +/- 140 -0.022781 0.0254037 0.086285 0.0282195 0.3021055 HLF hepatic leukemia factor 3087 +/- 125 2470 +/- 174 3295 +/- 234 3001 +/- 197 0.134433 -0.017992 -0.361245 0.0214442 0.5651557 HMBOX1 homeobox containing 1 2019 +/- 129 1029 +/- 14 1858 +/- 133 1219 +/- 270 0.225131 -0.000547 -0.078476 0.0022485 0.0116758 HMG2L1 high-mobility group protein 2-like 1 827 +/- 48 1078 +/- 75 788 +/- 50 931 +/- 40 -0.119134 0.0208691 0.052829 0.0053343 0.098226 HMGCR 3-hydroxy-3-methylglutaryl-Coenzyme A reductase 1890 +/- 172 1539 +/- 69 2130 +/- 95 1817 +/- 102 0.047367 -0.101924 -0.052681 0.0647459 0.186102 HMGCS2 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) 244 +/- 51 182 +/- 18 480 +/- 52 240 +/- 77 0.015867 -0.292636 -0.035266 0.5851959 0.0341019 HMGN2 high-mobility group nucleosomal binding domain 2 197 +/- 23 265 +/- 10 221 +/- 5 255 +/- 33 0.519438 0.0313723 0.376562 0.0222216 0.6900252 HNMT histamine N-methyltransferase 396 +/- 32 235 +/- 22 494 +/- 59 338 +/- 17 0.006664 -0.002524 -0.044935 0.0213492 0.0723774 HNRPA0 Heterogeneous nuclear ribonucleoprotein A0 336 +/- 12 561 +/- 28 394 +/- 18 576 +/- 23 0.107938 0.0001989 0.000242 0.0094807 0.0192505 HNRPA3P1heterogeneous nuclear ribonucleoprotein A3 pseudogene 1 1370 +/- 59 1182 +/- 49 1527 +/- 55 1349 +/- 65 0.026639 -0.033981 -0.067248 0.1591375 0.2003319 HNRPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa 1406 +/- 64 1586 +/- 51 1838 +/- 60 1825 +/- 128 0.000959 0.0542112 -0.927465 0.2100338 0.9676209 HNRPH3 heterogeneous nuclear ribonucleoprotein H3 (2H9) 973 +/- 64 1240 +/- 83 1247 +/- 84 1322 +/- 104 0.019894 0.0303551 0.590177 0.0251709 0.9298828 HNRPL heterogeneous nuclear ribonucleoprotein L 5232 +/- 292 6250 +/- 170 6161 +/- 225 7259 +/- 144 0.000633 0.0167305 0.003234 0.1424168 0.0232842 HOMER1 homer homolog 1 (Drosophila) 2901 +/- 169 2385 +/- 132 2363 +/- 91 2257 +/- 80 -0.023078 -0.038188 -0.408436 0.0448595 0.3022804 HP1BP3 heterochromatin protein 1, binding protein 3 5237 +/- 233 4163 +/- 95 5620 +/- 224 4368 +/- 255 0.136307 -0.004208 -0.005507 0.0308372 0.0730441 HRASLS3 HRAS-like suppressor 3 3691 +/- 429 4571 +/- 653 2117 +/- 93 2770 +/- 179 -0.001697 0.290076 0.017368 0.1218947 0.0152259 HRH1 Histamine receptor H1 110 +/- 13 169 +/- 16 148 +/- 14 93 +/- 20 -0.627131 0.0178774 -0.055363 0.0742791 0.0269023 HS1BP3 HS1-binding protein 3 323 +/- 18 374 +/- 24 190 +/- 32 362 +/- 29 -0.023458 0.1289462 0.0032 0.3309578 0.0331526 HSD11B1 hydroxysteroid (11-beta) dehydrogenase 1 182 +/- 39 214 +/- 25 78 +/- 15 136 +/- 13 -0.009558 0.5032791 0.015016 0.5861892 0.0606506 HSD17B6 hydroxysteroid (17-beta) dehydrogenase 6 3312 +/- 301 2181 +/- 99 2842 +/- 167 1796 +/- 97 -0.003708 -0.011557 -0.00067 0.0240631 0.0116791 HSDL2 hydroxysteroid dehydrogenase like 2 117 +/- 13 171 +/- 18 115 +/- 10 148 +/- 16 -0.684815 0.0367751 0.129427 0.085526 0.0049882 HSFY1 heat shock transcription factor, Y-linked 1 126 +/- 19 179 +/- 15 169 +/- 7 138 +/- 5 0.717732 0.0546282 -0.007341 0.0049516 0.0533977 HSMPP8 M-phase phosphoprotein, mpp8 1015 +/- 35 1210 +/- 68 1054 +/- 61 1091 +/- 69 -0.903502 0.0355074 0.696141 0.0074211 0.4485377 HSP90AB1heat shock protein 90kDa alpha (cytosolic), class B member 1 11071 +/- 195 13271 +/- 831 9909 +/- 375 10714 +/- 222 -0.006433 0.0450899 0.102338 0.1705916 0.0942842 HSP90B1 heat shock protein 90kDa beta (Grp94), member 1 5233 +/- 258 4774 +/- 119 5615 +/- 180 4876 +/- 111 0.109294 -0.150045 -0.008041 0.4535918 0.200611 HSPA12A heat shock 70kDa protein 12A 420 +/- 40 297 +/- 27 455 +/- 42 300 +/- 28 0.471796 -0.030854 -0.015229 0.0541254 0.100143 HSPB8 heat shock 22kDa protein 8 2202 +/- 213 3231 +/- 299 2037 +/- 225 2797 +/- 223 -0.014424 0.0205075 0.040316 0.0801769 0.3845944 HSPC159 HSPC159 protein 145 +/- 22 117 +/- 17 161 +/- 15 118 +/- 10 0.821222 -0.332804 -0.042647 0.2118259 0.0564253 HSPC176 hematopoietic stem/progenitor cells 176 666 +/- 60 863 +/- 48 612 +/- 77 712 +/- 53 -0.184744 0.0290196 0.316878 0.2618604 0.2751186 HSPE1 heat shock 10kDa protein 1 (chaperonin 10) 3558 +/- 135 3064 +/- 138 3300 +/- 90 2930 +/- 94 -0.054454 -0.028255 -0.019469 0.0466405 0.0305899

S1- alphabetical list of aging-related genes.xls Page 14 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 166 +/- 21 103 +/- 13 182 +/- 25 126 +/- 12 0.045284 -0.03231 -0.078401 0.2517837 0.1153546 HYLS1 hydrolethalus syndrome 1 748 +/- 26 632 +/- 33 635 +/- 12 572 +/- 47 -0.002791 -0.020014 -0.253758 0.0580063 0.9635505 IARS2 isoleucine-tRNA synthetase 2, mitochondrial 656 +/- 36 831 +/- 33 634 +/- 50 784 +/- 31 -0.111969 0.004645 0.033032 0.0003749 0.0058128 IBRDC3 IBR domain containing 3 179 +/- 43 292 +/- 22 217 +/- 41 344 +/- 26 0.004416 0.0476291 0.029838 0.2191918 0.2995504 ICA1L islet cell autoantigen 1,69kDa-like 791 +/- 58 1295 +/- 113 876 +/- 42 1345 +/- 82 0.081592 0.0046744 0.002173 0.0466876 0.0171912 ICAM1 intercellular adhesion molecule 1 (CD54), human rhinovirus receptor 120 +/- 10 185 +/- 13 126 +/- 9 167 +/- 8 -0.509902 0.0027757 0.007974 0.0012246 0.1861653 IDH3B isocitrate dehydrogenase 3 (NAD+) beta 1428 +/- 48 1204 +/- 29 1103 +/- 38 1036 +/- 36 -0.00011 -0.003798 -0.233414 0.0286207 0.7010248 IFIT3 interferon-induced protein with tetratricopeptide repeats 3 309 +/- 18 383 +/- 25 287 +/- 18 321 +/- 15 -0.051765 0.0397857 0.17307 0.0204367 0.3699558 IFIT5 interferon-induced protein with tetratricopeptide repeats 5 467 +/- 26 398 +/- 26 493 +/- 24 422 +/- 16 0.29216 -0.091013 -0.035868 0.1658923 0.2210693 IFNAR1 interferon (alpha, beta and omega) receptor 1 514 +/- 18 825 +/- 71 497 +/- 23 630 +/- 59 -0.027489 0.0063634 0.088126 0.0011211 0.1302925 IFNAR2 interferon (alpha, beta and omega) receptor 2 189 +/- 22 286 +/- 18 155 +/- 25 256 +/- 36 -0.140271 0.0071881 0.051828 0.0403499 0.1299888 IFNGR1 interferon gamma receptor 1 1775 +/- 52 2011 +/- 102 1350 +/- 54 1527 +/- 56 -0.000166 0.0756206 0.049648 0.0313991 0.0954485 IFT140 intraflagellar transport 140 homolog (Chlamydomonas) 212 +/- 12 263 +/- 17 244 +/- 23 276 +/- 16 0.045038 0.0372744 0.272873 0.071048 0.3425192 IGBP1 immunoglobulin (CD79A) binding protein 1 1965 +/- 47 2506 +/- 69 1885 +/- 71 1954 +/- 88 -0.021218 0.0001316 0.556128 0.0088608 0.3758762 IGF1R Insulin-like growth factor 1 receptor 545 +/- 60 811 +/- 50 668 +/- 61 725 +/- 86 0.402059 0.0071628 0.608119 0.0077351 0.1993183 IGF2 insulin-like growth factor 2 (somatomedin A) 536 +/- 149 327 +/- 80 242 +/- 33 429 +/- 64 -0.525197 -0.252466 0.040788 0.1133974 0.1150699 IGFBP5 insulin-like growth factor binding protein 5 174 +/- 19 323 +/- 54 358 +/- 138 309 +/- 76 0.85311 0.0392256 -0.763078 0.0056486 0.9501952 IGSF11 immunoglobulin superfamily, member 11 1835 +/- 127 2330 +/- 191 1702 +/- 60 1916 +/- 70 -0.044071 0.0602246 0.048375 0.0127626 0.0923113 IGSF9 immunoglobulin superfamily, member 9 140 +/- 15 213 +/- 22 159 +/- 9 169 +/- 13 -0.457673 0.0209872 0.530616 0.0624533 0.1719082 IKBKAP inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated pro 3512 +/- 159 3207 +/- 197 3071 +/- 108 2708 +/- 98 -0.000191 -0.257208 -0.034273 0.9879113 0.3691163 IL13RA1 interleukin 13 receptor, alpha 1 147 +/- 22 136 +/- 18 121 +/- 7 176 +/- 7 0.760091 -0.703974 0.000284 0.7379283 0.0454232 INADL InaD-like (Drosophila) 87 +/- 10 135 +/- 10 146 +/- 6 143 +/- 14 0.010416 0.0084226 -0.845084 0.0070272 0.4992556 INSIG1 insulin induced gene 1 1796 +/- 160 2510 +/- 276 2251 +/- 175 2909 +/- 224 0.144515 0.0554641 0.049265 0.0828132 0.4063671 IPLA2(GAMintracellular membrane-associated calcium-independent phospholipase A2 gamma 3034 +/- 116 2924 +/- 99 3184 +/- 174 2707 +/- 92 -0.886454 -0.488736 -0.043352 0.8441567 0.1281932 IPO9 importin 9 1211 +/- 95 1365 +/- 81 1369 +/- 47 1573 +/- 68 0.010492 0.2437822 0.042275 0.2746075 0.0033788 ISG15 ISG15 ubiquitin-like modifier 312 +/- 25 375 +/- 30 216 +/- 17 298 +/- 16 -0.001794 0.1427383 0.007132 0.1085195 0.2043022 ISG20L1 interferon stimulated exonuclease gene 20kDa-like 1 320 +/- 14 256 +/- 21 292 +/- 17 271 +/- 4 -0.540022 -0.034363 -0.270545 0.3314558 0.5291386 ITCH itchy homolog E3 ubiquitin protein ligase (mouse) 198 +/- 29 78 +/- 10 178 +/- 21 75 +/- 12 -0.536965 -0.007101 -0.002595 0.061654 0.0227736 ITFG1 Integrin alpha FG-GAP repeat containing 1 7600 +/- 215 6251 +/- 221 6538 +/- 483 6093 +/- 241 -0.020899 -0.001403 -0.437198 0.0083954 0.6222976 ITGA6 integrin, alpha 6 1277 +/- 56 1145 +/- 70 1213 +/- 98 804 +/- 50 -0.066816 -0.173709 -0.00691 0.6177074 0.0750815 ITGA8 integrin, alpha 8 101 +/- 17 101 +/- 14 126 +/- 12 92 +/- 3 0.133125 -0.982265 -0.035995 0.9156943 0.1779205 ITGAM integrin, alpha M (complement component 3 receptor 3 subunit) 332 +/- 23 412 +/- 12 323 +/- 26 348 +/- 34 -0.171997 0.0167668 0.584584 0.0840144 0.5527492 ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK1 657 +/- 64 790 +/- 95 641 +/- 35 744 +/- 25 -0.375166 0.2811003 0.041142 0.1133481 0.0789991 ITGB1BP1 integrin beta 1 binding protein 1 832 +/- 41 713 +/- 53 853 +/- 30 743 +/- 28 0.744054 -0.108727 -0.025875 0.0989607 0.1395426 ITGB4 integrin, beta 4 433 +/- 101 626 +/- 98 854 +/- 90 1205 +/- 70 4.03E-05 0.1993225 0.013637 0.3119505 0.1281018 ITIH5 inter-alpha (globulin) inhibitor H5 396 +/- 40 251 +/- 36 310 +/- 50 237 +/- 35 -0.111694 -0.023919 -0.263543 0.0161808 0.1563735 ITM2A integral membrane protein 2A 1463 +/- 86 1990 +/- 49 1400 +/- 32 1806 +/- 148 -0.175187 0.0007281 0.050001 0.0209382 0.2477824 IVNS1ABP influenza virus NS1A binding protein 993 +/- 80 1097 +/- 80 745 +/- 17 848 +/- 36 -0.000147 0.3783459 0.043123 0.2703699 0.0029402 JMJD4 jumonji domain containing 4 451 +/- 27 321 +/- 25 448 +/- 15 402 +/- 26 0.150407 -0.005768 -0.172553 0.0117518 0.3260716 KARCA1 kelch/ankyrin repeat containing cyclin A1 interacting protein 2299 +/- 44 1908 +/- 148 2213 +/- 74 1909 +/- 113 -0.414643 -0.045072 -0.058893 0.0134478 0.0133977 KBTBD3 kelch repeat and BTB (POZ) domain containing 3 245 +/- 17 231 +/- 14 224 +/- 11 186 +/- 9 -0.00714 -0.559314 -0.027498 0.8803297 0.0445753 KCMF1 potassium channel modulatory factor 1 904 +/- 45 798 +/- 43 861 +/- 25 761 +/- 23 -0.410119 -0.117733 -0.018055 0.1185997 0.1866276 KCNA5 potassium voltage-gated channel, shaker-related subfamily, member 5 160 +/- 33 140 +/- 13 180 +/- 11 124 +/- 7 0.926943 -0.581877 -0.002309 0.9751667 0.0322322 KCNH2 potassium voltage-gated channel, subfamily H (eag-related), member 2 579 +/- 45 458 +/- 18 529 +/- 26 467 +/- 29 -0.641046 -0.043132 -0.147815 0.1007191 0.5059796

S1- alphabetical list of aging-related genes.xls Page 15 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG KCNIP1 Kv channel interacting protein 1 233 +/- 13 189 +/- 12 243 +/- 17 208 +/- 14 0.497706 -0.03342 -0.148767 0.2405216 0.0271914 KCNJ16 potassium inwardly-rectifying channel, subfamily J, member 16 132 +/- 23 102 +/- 14 178 +/- 23 116 +/- 14 0.010593 -0.296493 -0.050114 0.2193097 0.0426232 KCNK17 potassium channel, subfamily K, member 17 175 +/- 17 111 +/- 10 253 +/- 20 177 +/- 20 0.000433 -0.011002 -0.023816 0.0727271 0.0841288 KCNK2 potassium channel, subfamily K, member 2 130 +/- 38 100 +/- 18 109 +/- 11 75 +/- 3 -0.23212 -0.483793 -0.02277 0.257196 0.0298694 KCNQ2 potassium voltage-gated channel, KQT-like subfamily, member 2 279 +/- 12 234 +/- 12 261 +/- 17 237 +/- 14 -0.756787 -0.025523 -0.286495 0.170292 0.331447 KCTD17 potassium channel tetramerisation domain containing 17 288 +/- 31 189 +/- 16 389 +/- 31 381 +/- 41 0.003133 -0.022214 -0.885017 0.0242203 0.706816 KCTD3 potassium channel tetramerisation domain containing 3 1153 +/- 87 1018 +/- 49 1352 +/- 65 947 +/- 142 0.354361 -0.213512 -0.043041 0.556885 0.316617 KHDRBS1 KH domain containing, RNA binding, signal transduction associated 1 434 +/- 12 512 +/- 23 424 +/- 28 401 +/- 15 -0.035547 0.0168749 -0.478305 0.3175819 0.3900469 KIAA1559 mouse zinc finger protein 14-like 130 +/- 17 122 +/- 12 158 +/- 9 116 +/- 6 0.142807 -0.731879 -0.003437 0.5966349 0.0162574 KIDINS220Kinase D-interacting substance of 220 kDa 221 +/- 98 520 +/- 82 208 +/- 72 376 +/- 75 -0.033925 0.0420293 0.142025 0.1127798 0.3116226 KIF21A kinesin family member 21A 3821 +/- 217 2757 +/- 229 4422 +/- 302 3119 +/- 95 0.000296 -0.007043 -0.00637 0.0635413 0.0334699 KIF21B kinesin family member 21B 3015 +/- 342 2089 +/- 271 2816 +/- 158 2164 +/- 78 -0.802722 -0.060923 -0.007346 0.0726565 0.0210055 KIF26B kinesin family member 26B 192 +/- 20 165 +/- 9 221 +/- 16 158 +/- 13 0.368792 -0.247789 -0.012319 0.1008889 0.042473 KIF3A kinesin family member 3A 6022 +/- 351 7526 +/- 490 5756 +/- 353 7952 +/- 415 0.932262 0.0338848 0.003446 0.4477694 0.1042408 KIF3B kinesin family member 3B 1592 +/- 199 1106 +/- 96 1784 +/- 164 1327 +/- 44 0.033434 -0.062974 -0.037931 0.1085254 0.1408668 KIF3C kinesin family member 3C 5625 +/- 458 4054 +/- 161 4195 +/- 251 3767 +/- 99 -0.012429 -0.016957 -0.16042 0.0051181 0.2302338 KIF5A kinesin family member 5A 2488 +/- 627 4967 +/- 527 2216 +/- 519 3839 +/- 250 -0.025483 0.0131554 0.025515 0.0698021 0.1681597 KLF13 Kruppel-like factor 13 1790 +/- 60 1158 +/- 151 2000 +/- 133 1313 +/- 196 0.021147 -0.006851 -0.02201 0.1488425 0.3862592 KLHDC5 kelch domain containing 5 3067 +/- 144 2879 +/- 162 2960 +/- 80 2641 +/- 82 -0.059624 -0.405494 -0.02161 0.2110651 0.0813829 KLHL15 kelch-like 15 (Drosophila) 646 +/- 36 564 +/- 39 642 +/- 26 552 +/- 15 -0.838295 -0.154466 -0.016239 0.1567848 0.0784295 KLHL2 kelch-like 2, Mayven (Drosophila) 4816 +/- 510 3890 +/- 688 7319 +/- 565 5661 +/- 464 0.000996 -0.306975 -0.049757 0.4627157 0.301802 KLHL21 kelch-like 21 (Drosophila) 1952 +/- 122 1902 +/- 103 1702 +/- 32 1533 +/- 45 -0.00605 -0.762969 -0.016513 0.7908324 0.0086259 KLHL8 kelch-like 8 (Drosophila) 1978 +/- 100 2286 +/- 94 2118 +/- 103 2280 +/- 170 0.594119 0.0492286 0.442491 0.2317898 0.5971461 KPNA1 karyopherin alpha 1 (importin alpha 5) 2311 +/- 102 1982 +/- 79 2132 +/- 68 2092 +/- 86 -0.346058 -0.030322 -0.725154 0.0426209 0.9721896 KPNA3 karyopherin alpha 3 (importin alpha 4) 2725 +/- 82 2246 +/- 35 2709 +/- 42 2343 +/- 59 0.394828 -0.001187 -0.001242 0.0066767 0.0312176 KRR1 KRR1, small subunit (SSU) processome component, homolog (yeast) 143 +/- 22 372 +/- 77 168 +/- 37 387 +/- 79 0.592803 0.0303006 0.048322 0.2824591 0.3277337 KRTCAP2 Keratinocyte associated protein 2 159 +/- 10 120 +/- 17 200 +/- 25 128 +/- 5 0.096235 -0.08101 -0.033837 0.0763629 0.1569348 KTN1 kinectin 1 (kinesin receptor) 3032 +/- 249 3355 +/- 275 3498 +/- 199 2780 +/- 142 -0.732438 0.4031136 -0.017362 0.1279059 0.2641368 Kua ubiquitin-conjugating enzyme variant Kua 968 +/- 77 876 +/- 70 858 +/- 38 702 +/- 42 -0.020679 -0.400916 -0.022812 0.6755094 0.0203844 LARP1 La ribonucleoprotein domain family, member 1 2654 +/- 61 2210 +/- 101 2989 +/- 126 2762 +/- 90 0.000182 -0.005348 -0.178466 0.0044175 0.1765114 LASP1 LIM and SH3 protein 1 3715 +/- 152 3291 +/- 108 3992 +/- 179 3563 +/- 143 0.122979 -0.048664 -0.094855 0.0526266 0.4095864 LASS2 LAG1 longevity assurance homolog 2 (S. cerevisiae) 1543 +/- 159 2019 +/- 247 1012 +/- 58 1354 +/- 79 -0.005517 0.1417419 0.008589 0.0146985 0.0166976 LASS5 LAG1 longevity assurance homolog 5 (S. cerevisiae) 1328 +/- 43 993 +/- 51 1277 +/- 72 1037 +/- 33 -0.695083 -0.000566 -0.019 0.0004572 0.1274037 LASS6 LAG1 longevity assurance homolog 6 (S. cerevisiae) 241 +/- 14 185 +/- 9 233 +/- 20 237 +/- 21 0.248882 -0.009063 0.911095 0.0018281 0.6992862 LAT2 linker for activation of T cells family, member 2 246 +/- 22 214 +/- 23 266 +/- 22 203 +/- 17 0.630977 -0.346152 -0.047563 0.6063961 0.1040978 LAYN layilin 174 +/- 39 137 +/- 9 89 +/- 5 120 +/- 9 -0.030574 -0.392233 0.018567 0.164778 0.2289666 LCN6 lipocalin 6 288 +/- 28 285 +/- 19 344 +/- 14 251 +/- 22 0.347287 -0.929214 -0.009157 0.5618894 0.0411434 LEAP-2 liver-expressed antimicrobial peptide 2 142 +/- 15 191 +/- 11 154 +/- 13 150 +/- 10 -0.441339 0.0251609 -0.775806 0.0087654 0.5451072 LENG9 leukocyte receptor cluster (LRC) member 9 234 +/- 30 234 +/- 18 289 +/- 10 218 +/- 13 0.314411 0.9943279 -0.003089 0.8686565 0.0145138 LEREPO4 likely ortholog of mouse immediate early response, erythropoietin 4 3868 +/- 230 3508 +/- 145 4093 +/- 95 3354 +/- 211 0.539991 -0.219625 -0.020663 0.2803356 0.1262894 LETM2 leucine zipper-EF-hand containing transmembrane protein 2 125 +/- 7 98 +/- 8 102 +/- 12 112 +/- 8 -0.676823 -0.025883 0.501709 0.098268 0.3344092 LETMD1 LETM1 domain containing 1 392 +/- 31 684 +/- 39 420 +/- 19 632 +/- 54 -0.999437 0.0002075 0.013536 0.0004107 0.0035636 LGMN legumain 2017 +/- 149 2333 +/- 156 1847 +/- 52 2339 +/- 106 -0.147583 0.1751615 0.006075 0.4551521 0.1335073 LHPP phospholysine phosphohistidine inorganic pyrophosphate phosphatase 1897 +/- 163 2175 +/- 138 1606 +/- 105 1935 +/- 93 -0.035074 0.2232212 0.043902 0.1423172 0.2170396

S1- alphabetical list of aging-related genes.xls Page 16 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG LIFR leukemia inhibitory factor receptor alpha 2392 +/- 272 3256 +/- 164 2200 +/- 139 2374 +/- 110 -0.023868 0.025646 0.350575 0.0182029 0.8117387 LIMS1 LIM and senescent cell antigen-like domains 1 815 +/- 63 1059 +/- 110 676 +/- 38 871 +/- 66 -0.017507 0.0901478 0.040073 0.0174023 0.0048156 LITAF lipopolysaccharide-induced TNF factor 1951 +/- 250 2975 +/- 472 1460 +/- 69 1769 +/- 112 -0.014996 0.0932603 0.052765 0.0133063 0.013689 LIX1L Lix1 homolog (mouse) like 1777 +/- 112 2141 +/- 118 1728 +/- 67 1848 +/- 69 -0.087621 0.0493095 0.244961 0.0814555 0.4486052 LNPEP leucyl/cystinyl aminopeptidase 2120 +/- 127 2540 +/- 116 2687 +/- 122 2808 +/- 110 0.003585 0.0351572 0.478574 0.1309289 0.6568302 LNX1 ligand of numb-protein X 1 2480 +/- 118 1905 +/- 184 2474 +/- 174 1901 +/- 108 -0.98299 -0.02857 -0.022991 0.0026034 0.1566009 LONPL peroxisomal LON protease like 691 +/- 82 856 +/- 45 737 +/- 44 891 +/- 39 0.347324 0.1154895 0.02841 0.079131 0.0767525 LPHN1 latrophilin 1 3273 +/- 149 2840 +/- 165 3901 +/- 181 3300 +/- 124 0.001197 -0.080279 -0.023978 0.0840345 0.2816035 LRBA LPS-responsive vesicle trafficking, beach and anchor containing 170 +/- 21 175 +/- 6 242 +/- 14 194 +/- 12 0.019703 0.8467267 -0.031553 0.6117714 0.0536147 LRFN5 Leucine rich repeat and fibronectin type III domain containing 5 378 +/- 17 288 +/- 38 451 +/- 36 256 +/- 16 0.310075 -0.066662 -0.001746 0.0415564 0.0325591 LRIG1 leucine-rich repeats and immunoglobulin-like domains 1 2933 +/- 304 3934 +/- 210 2719 +/- 203 3170 +/- 146 -0.057252 0.0242412 0.105787 0.0790443 0.6036524 LRP11 low density lipoprotein receptor-related protein 11 4775 +/- 167 4237 +/- 116 4754 +/- 143 4135 +/- 226 -0.765163 -0.026691 -0.054358 0.2136043 0.4776775 LRPPRC leucine-rich PPR-motif containing 4632 +/- 236 3748 +/- 142 4099 +/- 157 3800 +/- 34 -0.185503 -0.011934 -0.116118 0.0130121 0.2733722 LRRC28 leucine rich repeat containing 28 389 +/- 32 357 +/- 24 478 +/- 25 395 +/- 14 0.02138 -0.447189 -0.021643 0.3077422 0.1417298 LRRC37B leucine rich repeat containing 37B 85 +/- 12 114 +/- 6 139 +/- 7 97 +/- 7 0.12692 0.0633626 -0.002603 0.1591368 0.0191785 LRRC39 leucine rich repeat containing 39 91 +/- 6 115 +/- 9 98 +/- 6 108 +/- 9 0.900485 0.0438754 0.398742 0.1491985 0.5798223 LRRC3B leucine rich repeat containing 3B 1060 +/- 61 844 +/- 59 1402 +/- 91 1140 +/- 58 0.001031 -0.029331 -0.040665 0.0542119 0.2739959 LRRC8C Leucine rich repeat containing 8 family, member C 232 +/- 33 400 +/- 51 193 +/- 36 225 +/- 20 -0.039294 0.0233036 0.464991 0.0011553 0.528369 LRRFIP1 leucine rich repeat (in FLII) interacting protein 1 1529 +/- 101 1224 +/- 53 1604 +/- 56 1461 +/- 75 0.036357 -0.0299 -0.167583 0.0459792 0.224864 LRRN1 leucine rich repeat neuronal 1 2089 +/- 141 1602 +/- 137 2532 +/- 162 1964 +/- 83 0.003337 -0.032954 -0.015981 0.0543872 0.048776 LRRN3 leucine rich repeat neuronal 3 1738 +/- 84 1547 +/- 86 1796 +/- 57 1379 +/- 50 -0.873505 -0.142278 -0.000375 0.325884 0.0072199 LRRN5 leucine rich repeat neuronal 5 1461 +/- 68 1120 +/- 25 1554 +/- 45 1289 +/- 57 0.017935 -0.002904 -0.006257 0.0073701 0.0648862 LSM4 LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae) 1636 +/- 167 1825 +/- 54 1373 +/- 9 1750 +/- 75 -0.126485 0.3202361 0.007012 0.4957141 0.0036394 LSM5 LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) 860 +/- 61 808 +/- 19 1126 +/- 50 958 +/- 52 6.31E-05 -0.44519 -0.045762 0.5446384 0.0887925 LSM8 LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae) 638 +/- 35 457 +/- 26 600 +/- 28 455 +/- 21 -0.276978 -0.002364 -0.002654 0.1130698 0.0509296 LTB lymphotoxin beta (TNF superfamily, member 3) 150 +/- 32 249 +/- 30 143 +/- 12 161 +/- 19 -0.05276 0.0471031 0.446585 0.0280837 0.4278686 LTBP1 latent transforming growth factor beta binding protein 1 361 +/- 17 236 +/- 20 447 +/- 38 218 +/- 28 0.195132 -0.000715 -0.000959 0.0982468 0.0743978 LTC4S leukotriene C4 synthase 236 +/- 25 314 +/- 22 201 +/- 17 191 +/- 16 -0.013328 0.0419076 -0.662254 0.0265734 0.2643918 LUZP2 Leucine zipper protein 2 148 +/- 21 101 +/- 16 116 +/- 17 69 +/- 8 -0.002594 -0.10366 -0.035562 0.0774908 0.0654404 LYK5 protein kinase LYK5 1182 +/- 41 1209 +/- 34 1201 +/- 21 1266 +/- 17 0.393391 0.6225568 0.038831 0.5404764 0.0660318 LYPLA3 lysophospholipase 3 (lysosomal phospholipase A2) 288 +/- 21 365 +/- 24 331 +/- 23 339 +/- 18 0.341431 0.0344424 0.774489 0.1625305 0.8967138 LYPLAL1 Lysophospholipase-like 1 1724 +/- 216 2920 +/- 69 2065 +/- 232 3308 +/- 85 0.00125 0.0018445 0.002086 0.0113258 0.0342218 LYZ lysozyme (renal amyloidosis) 354 +/- 21 541 +/- 71 247 +/- 38 330 +/- 29 -0.002236 0.045574 0.117092 0.0080013 0.1612533 LZIC Leucine zipper and CTNNBIP1 domain containing 187 +/- 11 146 +/- 17 188 +/- 10 125 +/- 20 -0.614058 -0.073599 -0.033697 0.6669541 0.1506325 LZTR2 Leucine zipper transcription regulator 2 124 +/- 12 87 +/- 9 134 +/- 23 117 +/- 16 0.058076 -0.033319 -0.554797 0.0032105 0.1444458 MAD2L1BPMAD2L1 binding protein 801 +/- 48 804 +/- 14 744 +/- 14 700 +/- 10 -0.014653 0.9520305 -0.030987 0.8433146 0.1503947 MAD2L2 MAD2 mitotic arrest deficient-like 2 (yeast) 622 +/- 72 573 +/- 24 543 +/- 12 601 +/- 20 -0.397027 -0.546859 0.044277 0.4975125 0.2097003 MAGEE1 melanoma antigen family E, 1 1315 +/- 90 1045 +/- 85 1385 +/- 82 1313 +/- 89 0.032925 -0.054496 -0.568035 0.0144637 0.2644807 MAGEH1 melanoma antigen family H, 1 5195 +/- 193 4645 +/- 235 6391 +/- 306 5229 +/- 199 0.003344 -0.101413 -0.012417 0.1068453 0.0896327 MALAT1 metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA) 999 +/- 139 1887 +/- 224 1230 +/- 192 2019 +/- 444 0.09744 0.0092905 0.158901 0.0157138 0.2406337 MAML2 Mastermind-like 2 (Drosophila) 200 +/- 11 150 +/- 14 192 +/- 13 180 +/- 9 0.215953 -0.018402 -0.467975 0.0818255 0.3713773 MAN2A1 Mannosidase, alpha, class 2A, member 1 1559 +/- 217 2028 +/- 286 855 +/- 94 1116 +/- 57 -0.000591 0.2219283 0.044273 0.1878816 0.1437013 MANEAL mannosidase, endo-alpha-like 2351 +/- 85 2004 +/- 64 2117 +/- 72 1985 +/- 75 -0.041567 -0.009304 -0.239499 0.0043888 0.1250339 MAP1LC3Amicrotubule-associated protein 1 light chain 3 alpha 3967 +/- 274 2363 +/- 245 4800 +/- 253 2961 +/- 404 7.47E-05 -0.001442 -0.006366 0.0598346 0.1636142

S1- alphabetical list of aging-related genes.xls Page 17 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG MAP3K13 Mitogen-activated protein kinase kinase kinase 13 655 +/- 36 806 +/- 40 539 +/- 37 736 +/- 36 -0.003978 0.0192338 0.004278 0.1930077 0.0880737 MAP3K3 mitogen-activated protein kinase kinase kinase 3 365 +/- 26 320 +/- 15 396 +/- 23 317 +/- 17 0.421251 -0.179094 -0.021845 0.1388563 0.0452086 MAP3K6 mitogen-activated protein kinase kinase kinase 6 292 +/- 47 544 +/- 98 200 +/- 27 272 +/- 34 -0.006841 0.0514709 0.136153 0.0664212 0.0156765 MAP4K2 mitogen-activated protein kinase kinase kinase kinase 2 313 +/- 12 258 +/- 18 306 +/- 26 255 +/- 7 -0.83887 -0.032048 -0.101177 0.061029 0.3369089 MAP4K4 mitogen-activated protein kinase kinase kinase kinase 4 1103 +/- 94 1434 +/- 119 899 +/- 30 954 +/- 55 -0.002764 0.0546811 0.40866 0.0191518 0.1934536 MAP4K5 mitogen-activated protein kinase kinase kinase kinase 5 695 +/- 83 1080 +/- 188 670 +/- 51 919 +/- 70 -0.238071 0.1036578 0.021575 0.0090824 0.0035709 MAP6 microtubule-associated protein 6 1084 +/- 21 874 +/- 23 1109 +/- 50 972 +/- 50 0.114599 -4.96E-05 -0.084488 0.0035326 0.3205931 MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 4276 +/- 133 3711 +/- 134 3607 +/- 165 3762 +/- 73 -0.066483 -0.013644 0.420129 0.0263949 0.3424538 MAPK9 Mitogen-activated protein kinase 9 4733 +/- 223 3991 +/- 177 4745 +/- 208 4279 +/- 155 0.386593 -0.027089 -0.107048 0.0045747 0.528748 MAPRE1 microtubule-associated protein, RP/EB family, member 1 1826 +/- 135 2236 +/- 182 1327 +/- 73 1595 +/- 31 -0.000328 0.1033306 0.012844 0.0227255 0.0289238 MAPRE3 microtubule-associated protein, RP/EB family, member 3 3330 +/- 90 2945 +/- 89 3841 +/- 102 3654 +/- 41 7.86E-06 -0.012243 -0.134442 0.0240235 0.3335935 MARCH6 membrane-associated ring finger (C3HC4) 6 1489 +/- 19 1154 +/- 34 1345 +/- 50 1122 +/- 25 -0.028684 -2.87E-05 -0.004757 0.0050401 0.0443174 MARK2 MAP/microtubule affinity-regulating kinase 2 823 +/- 33 702 +/- 30 941 +/- 40 765 +/- 25 0.022501 -0.023036 -0.005707 0.1351836 0.132393 MASP1 mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive facto 248 +/- 14 181 +/- 18 233 +/- 11 210 +/- 10 0.400344 -0.013609 -0.157176 0.0765893 0.4725587 MAST4 microtubule associated serine/threonine kinase family member 4 163 +/- 7 143 +/- 21 168 +/- 2 203 +/- 10 0.09567 -0.408933 0.022908 0.2140955 0.0018216 MAT2B methionine adenosyltransferase II, beta 1924 +/- 121 3545 +/- 199 2623 +/- 156 3789 +/- 141 0.006021 9.944E-05 0.00036 0.0073799 0.0041307 MATR3 matrin 3 18535 +/- 672 15789 +/- 403 18512 +/- 661 16674 +/- 807 0.506425 -0.007754 -0.115262 0.0347903 0.2129205 MAX MYC associated factor X 1031 +/- 87 779 +/- 39 1103 +/- 54 911 +/- 39 0.098348 -0.034214 -0.019564 0.0674485 0.2076106 MBD5 methyl-CpG binding domain protein 5 1291 +/- 137 1781 +/- 99 1091 +/- 72 1304 +/- 87 -0.00565 0.0174222 0.094876 0.0314458 0.2979001 MBNL2 Muscleblind-like 2 (Drosophila) 431 +/- 36 550 +/- 27 566 +/- 22 603 +/- 44 0.010202 0.0275284 0.492126 0.0026829 0.0633907 MBP myelin basic protein 267 +/- 30 382 +/- 30 198 +/- 26 233 +/- 24 -0.002067 0.0221518 0.35689 0.1632095 0.6588283 MCAM melanoma cell adhesion molecule 1170 +/- 212 1662 +/- 293 589 +/- 48 817 +/- 61 -0.004321 0.2075345 0.018798 0.0884724 0.0316572 MCCC1 methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) 588 +/- 18 799 +/- 27 614 +/- 25 820 +/- 25 0.119145 0.0001339 0.000249 0.0255502 0.0645935 MCF2L MCF.2 cell line derived transforming sequence-like 169 +/- 11 113 +/- 10 232 +/- 24 135 +/- 8 0.008463 -0.003333 -0.007613 0.033675 0.0679351 MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein 113 +/- 18 175 +/- 17 138 +/- 12 186 +/- 14 0.320971 0.0344173 0.029902 0.1390213 0.017435 MCOLN1 mucolipin 1 869 +/- 34 752 +/- 20 733 +/- 41 707 +/- 21 -0.001182 -0.019151 -0.582762 0.1177468 0.6944621 MDH1B malate dehydrogenase 1B, NAD (soluble) 438 +/- 14 356 +/- 17 295 +/- 17 277 +/- 4 -0.000321 -0.004634 -0.33135 0.064732 0.4103282 MED19 mediator of RNA polymerase II transcription, subunit 19 homolog (S. cerevisiae) 1426 +/- 32 1306 +/- 36 1307 +/- 44 1177 +/- 45 -0.003276 -0.032648 -0.069495 0.2870689 0.2965897 MED6 mediator of RNA polymerase II transcription, subunit 6 homolog (S. cerevisiae) 256 +/- 21 344 +/- 26 281 +/- 19 322 +/- 15 0.403661 0.0259116 0.125755 0.0140672 0.1003775 MEGF10 multiple EGF-like-domains 10 775 +/- 88 1122 +/- 99 648 +/- 44 768 +/- 24 -0.025721 0.0259818 0.045696 0.0129927 0.2333869 MEIS3 Meis1, myeloid ecotropic viral integration site 1 homolog 3 (mouse) 654 +/- 42 481 +/- 45 714 +/- 138 476 +/- 75 0.528244 -0.019556 -0.170505 0.2113097 0.4813491 MEIS3P1 Meis1 homolog 3 (mouse) pseudogene 1 175 +/- 34 118 +/- 10 239 +/- 25 167 +/- 18 0.009456 -0.158737 -0.043823 0.2176017 0.0063298 MEST mesoderm specific transcript homolog (mouse) 2485 +/- 318 2364 +/- 191 3814 +/- 151 3349 +/- 120 0.00046 -0.752188 -0.039852 0.8600518 0.0791152 METT11D1methyltransferase 11 domain containing 1 227 +/- 25 163 +/- 12 203 +/- 17 195 +/- 18 0.931643 -0.049923 -0.753512 0.0643284 0.4893438 METTL5 methyltransferase like 5 537 +/- 35 732 +/- 38 613 +/- 18 740 +/- 26 0.227934 0.0036136 0.004301 0.0647282 0.1395292 METTL7A methyltransferase like 7A 306 +/- 63 569 +/- 51 420 +/- 87 661 +/- 102 0.006533 0.009059 0.107987 0.0092362 0.0722464 MFAP1 microfibrillar-associated protein 1 1476 +/- 22 1315 +/- 35 1367 +/- 41 1252 +/- 27 -0.012697 -0.004145 -0.047852 0.0023246 0.0629694 MFN2 mitofusin 2 4230 +/- 255 4976 +/- 285 3779 +/- 52 4823 +/- 275 -0.132154 0.0800305 0.017921 0.2288597 0.0157142 MGLL monoglyceride lipase 886 +/- 65 790 +/- 40 920 +/- 52 666 +/- 35 -0.472827 -0.24052 -0.003363 0.167516 0.0155586 MID1IP1 MID1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) 3282 +/- 363 4184 +/- 464 3175 +/- 236 3893 +/- 110 -0.500175 0.1584278 0.028131 0.0559358 0.0671908 MIER1 mesoderm induction early response 1 homolog (Xenopus laevis) 445 +/- 23 518 +/- 23 603 +/- 34 526 +/- 34 0.029382 0.0521286 -0.13956 0.1971887 0.0703255 MKKS McKusick-Kaufman syndrome 2005 +/- 127 1739 +/- 127 2049 +/- 26 1793 +/- 93 0.347105 -0.169457 -0.049556 0.3633382 0.1914514 MKLN1 muskelin 1, intracellular mediator containing kelch motifs 727 +/- 42 901 +/- 60 770 +/- 27 780 +/- 19 -0.507414 0.0406878 0.759906 0.0249527 0.9913302 MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) 569 +/- 45 480 +/- 41 470 +/- 32 363 +/- 16 -0.005193 -0.174971 -0.019893 0.7383671 0.0561074

S1- alphabetical list of aging-related genes.xls Page 18 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG MLL5 myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila) 1973 +/- 306 931 +/- 182 2063 +/- 365 760 +/- 108 -0.799589 -0.018738 -0.014619 0.0601887 0.038758 MLLT10 Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to 261 +/- 16 374 +/- 37 406 +/- 32 426 +/- 73 0.008615 0.0273662 0.811362 0.0087313 0.4124782 MNS1 meiosis-specific nuclear structural 1 261 +/- 32 177 +/- 16 182 +/- 17 182 +/- 19 -0.258874 -0.048126 0.990468 0.0778357 0.9215701 MOBKL2A MOB1, Mps One Binder kinase activator-like 2A (yeast) 166 +/- 17 108 +/- 15 199 +/- 13 144 +/- 22 0.03428 -0.030366 -0.067489 0.1776544 0.0137142 MOBKL2B MOB1, Mps One Binder kinase activator-like 2B (yeast) 138 +/- 10 100 +/- 11 127 +/- 7 86 +/- 4 -0.094381 -0.028522 -0.001404 0.0629582 0.0559199 MOBP myelin-associated oligodendrocyte basic protein 3329 +/- 750 6661 +/- 1634 1660 +/- 465 3347 +/- 458 -0.029555 0.106168 0.029747 0.0163244 0.0573451 MORC2 MORC family CW-type zinc finger 2 239 +/- 17 310 +/- 23 255 +/- 14 259 +/- 19 -0.537671 0.0330106 0.847917 0.1445579 0.5493222 MOSPD2 motile sperm domain containing 2 378 +/- 37 537 +/- 58 304 +/- 24 339 +/- 26 -0.01698 0.0480053 0.356208 0.001366 0.4828524 MPEG1 macrophage expressed gene 1 612 +/- 60 826 +/- 70 399 +/- 27 583 +/- 25 -0.001424 0.0432552 0.000738 0.0122355 0.0359659 MPP5 membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) 1226 +/- 50 1391 +/- 42 1232 +/- 32 1396 +/- 65 0.953747 0.0313973 0.065186 0.0737731 0.4395924 MPP6 membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) 324 +/- 28 239 +/- 22 340 +/- 27 328 +/- 27 0.053454 -0.038754 -0.752353 0.0603883 0.912007 MRFAP1 Mof4 family associated protein 1 14404 +/- 697 16399 +/- 660 13724 +/- 840 16337 +/- 665 -0.332911 0.0644536 0.037762 0.0522461 0.0649041 M-RIP myosin phosphatase-Rho interacting protein 3617 +/- 157 3133 +/- 138 3127 +/- 109 2889 +/- 119 -0.019774 -0.043121 -0.177579 0.0014899 0.3564228 MRP63 mitochondrial ribosomal protein 63 1794 +/- 66 1575 +/- 30 1880 +/- 76 1746 +/- 80 0.092583 -0.01964 -0.257592 0.0421543 0.3046604 MRPL20 mitochondrial ribosomal protein L20 2020 +/- 172 2410 +/- 102 1610 +/- 52 1867 +/- 59 -0.000857 0.0863428 0.010571 0.1739134 0.0151383 MRPL22 mitochondrial ribosomal protein L22 706 +/- 20 652 +/- 26 641 +/- 17 556 +/- 21 -0.001229 -0.128265 -0.014089 0.2421444 0.2526271 MRPL54 mitochondrial ribosomal protein L54 455 +/- 104 726 +/- 46 610 +/- 107 982 +/- 113 0.004144 0.049292 0.041066 0.0545014 0.0413921 MRPS15 mitochondrial ribosomal protein S15 1027 +/- 36 1142 +/- 68 917 +/- 42 1056 +/- 29 -0.056432 0.1731679 0.02398 0.1192765 0.1058852 MRPS22 mitochondrial ribosomal protein S22 836 +/- 43 995 +/- 69 810 +/- 40 988 +/- 24 -0.212622 0.0845011 0.005432 0.1050563 0.0043636 MRPS7 mitochondrial ribosomal protein S7 629 +/- 23 658 +/- 22 625 +/- 13 709 +/- 21 0.29472 0.3882636 0.011889 0.7491648 0.1191748 MRRF mitochondrial ribosome recycling factor 420 +/- 20 360 +/- 12 352 +/- 20 323 +/- 20 -0.019024 -0.032655 -0.331146 0.0505642 0.376247 MSTO1 misato homolog 1 (Drosophila) 494 +/- 31 687 +/- 43 490 +/- 34 667 +/- 50 -0.82405 0.00503 0.021227 0.0543974 0.2613201 MT1X metallothionein 1X 5805 +/- 490 4035 +/- 332 4142 +/- 463 3622 +/- 294 -0.003586 -0.015631 -0.370711 0.0501186 0.8429125 MTG1 mitochondrial GTPase 1 homolog (S. cerevisiae) 1704 +/- 61 1474 +/- 43 1403 +/- 55 1356 +/- 42 -0.00047 -0.01262 -0.51801 0.0267037 0.549807 MTHFD2L methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like 641 +/- 53 440 +/- 16 569 +/- 21 415 +/- 23 -0.110664 -0.010974 -0.000814 0.0829861 0.0236995 MUM1 melanoma associated antigen (mutated) 1 419 +/- 34 814 +/- 85 436 +/- 18 624 +/- 61 -0.209137 0.0042012 0.035077 0.0063943 0.0028992 MXI1 MAX interactor 1 6342 +/- 614 7312 +/- 652 4531 +/- 138 5071 +/- 166 -0.000504 0.3040709 0.036293 0.11012 0.0351373 MXRA7 matrix-remodelling associated 7 203 +/- 23 119 +/- 18 218 +/- 27 164 +/- 25 0.017414 -0.018307 -0.17431 0.0471686 0.2162351 MYADM myeloid-associated differentiation marker 444 +/- 27 337 +/- 28 548 +/- 77 393 +/- 23 0.064807 -0.020701 -0.10446 0.0197366 0.1079235 MYBBP1A MYB binding protein (P160) 1a 227 +/- 13 300 +/- 10 267 +/- 21 271 +/- 13 0.741657 0.0015408 0.877027 0.007627 0.8952852 MYEOV2 myeloma overexpressed 2 11575 +/- 867 12859 +/- 629 9590 +/- 539 11808 +/- 395 -0.019511 0.2608522 0.009414 0.3886535 0.0341309 MYL6 myosin, light polypeptide 6, alkali, smooth muscle and non-muscle 5381 +/- 175 5499 +/- 158 4330 +/- 103 4685 +/- 116 -1.69E-05 0.6286577 0.048931 0.9036789 0.209868 MYLK myosin, light polypeptide kinase 2425 +/- 309 3340 +/- 241 1446 +/- 132 2443 +/- 131 -0.000588 0.043083 0.000478 0.1166348 0.0693647 MYO1B myosin IB 240 +/- 40 180 +/- 30 189 +/- 25 121 +/- 13 -0.019778 -0.250919 -0.045613 0.4635443 0.0741961 MYSM1 myb-like, SWIRM and MPN domains 1 1621 +/- 104 1918 +/- 60 1905 +/- 135 2056 +/- 106 0.103681 0.0388338 0.400279 0.0991938 0.1981637 MYST1 MYST histone acetyltransferase 1 110 +/- 7 152 +/- 8 123 +/- 16 139 +/- 15 0.79701 0.0039066 0.484767 0.0917782 0.4486188 N4BP1 Nedd4 binding protein 1 375 +/- 24 511 +/- 28 401 +/- 42 438 +/- 25 -0.70576 0.0045694 0.473765 0.004723 0.3522507 N4BP3 Nedd4 binding protein 3 109 +/- 7 101 +/- 7 119 +/- 9 90 +/- 4 -0.532155 -0.409775 -0.022975 0.5139064 0.1388443 NADSYN1 NAD synthetase 1 338 +/- 32 449 +/- 39 242 +/- 20 262 +/- 19 -0.000626 0.054237 0.498735 0.0355291 0.7809915 NAG neuroblastoma-amplified protein 98 +/- 12 158 +/- 21 165 +/- 17 192 +/- 26 0.046839 0.0385576 0.425889 0.014356 0.4542652 NAG8 nasopharyngeal carcinoma associated gene protein-8 312 +/- 11 399 +/- 15 334 +/- 33 363 +/- 26 -0.98914 0.0013178 0.517727 0.0054183 0.419765 NAP1L1 nucleosome assembly protein 1-like 1 1463 +/- 33 1829 +/- 95 1400 +/- 79 1475 +/- 74 -0.038209 0.0102 0.499868 0.0008993 0.2561711 NAP5 Nck-associated protein 5 211 +/- 31 212 +/- 27 162 +/- 11 123 +/- 8 -0.011994 0.97362 -0.021145 0.6593897 0.1115944 NAPB N-ethylmaleimide-sensitive factor attachment protein, beta 177 +/- 26 406 +/- 74 348 +/- 67 475 +/- 68 0.013533 0.0261124 0.218482 0.1134007 0.3241578

S1- alphabetical list of aging-related genes.xls Page 19 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG NARFL nuclear prelamin A recognition factor-like 763 +/- 44 946 +/- 80 767 +/- 41 988 +/- 52 0.278881 0.0815146 0.010085 0.264246 0.1347152 NASP Nuclear autoantigenic sperm protein (histone-binding) 466 +/- 56 813 +/- 84 458 +/- 42 736 +/- 35 -0.070871 0.0078378 0.000662 0.0060059 0.0010065 NAT5 N-acetyltransferase 5 5238 +/- 136 4713 +/- 92 5091 +/- 104 4770 +/- 113 -0.579759 -0.011364 -0.067795 0.0215746 0.2718969 NBR1 neighbor of BRCA1 gene 1 1100 +/- 31 1283 +/- 49 1205 +/- 34 1320 +/- 16 0.027118 0.0122479 0.018009 0.0015849 0.086231 NCAM1 neural cell adhesion molecule 1 374 +/- 80 1218 +/- 237 262 +/- 43 746 +/- 266 -0.031607 0.014441 0.142824 0.0932677 0.5633117 NCKIPSD NCK interacting protein with SH3 domain 1420 +/- 128 2242 +/- 157 1411 +/- 94 2164 +/- 111 -0.57819 0.0024476 0.000745 0.0937244 0.0319407 NCOA5 nuclear receptor coactivator 5 279 +/- 3 222 +/- 19 348 +/- 18 305 +/- 13 6.26E-05 -0.02781 -0.088613 0.0763502 0.2254017 NDRG1 N-myc downstream regulated gene 1 6429 +/- 896 7904 +/- 978 4264 +/- 354 5467 +/- 340 -0.011262 0.2923671 0.036858 0.1415015 0.025735 NDUFA1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa 12545 +/- 378 10794 +/- 469 11641 +/- 107 11222 +/- 234 -0.624295 -0.016288 -0.157323 0.0037112 0.0136428 NDUFB10 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa 397 +/- 30 271 +/- 22 317 +/- 19 301 +/- 26 -0.284404 -0.007124 -0.622555 0.0041018 0.2428042 NDUFB2 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa 2656 +/- 38 2528 +/- 107 2217 +/- 39 2426 +/- 38 -0.003763 -0.300337 0.003892 0.0102589 0.1145787 NDUFB8 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa 11769 +/- 658 13321 +/- 391 9711 +/- 250 12186 +/- 194 -0.00071 0.0763743 2.97E-05 0.311303 0.0182935 NDUFC1 NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa 6197 +/- 241 5433 +/- 247 6237 +/- 149 5646 +/- 145 0.370562 -0.05122 -0.019431 0.0329258 0.0326521 NDUFS2 NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) 2696 +/- 97 2386 +/- 111 2750 +/- 69 2421 +/- 74 0.905299 -0.062112 -0.010541 0.0223896 0.012268 NDUFV1 NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa 2923 +/- 155 2506 +/- 143 2595 +/- 65 2288 +/- 108 -0.051091 -0.076226 -0.045771 0.1271505 0.2553183 NECAP2 NECAP endocytosis associated 2 1224 +/- 129 1647 +/- 142 993 +/- 53 1204 +/- 15 -0.006308 0.0523344 0.009162 0.0413594 0.0504406 NEDD9 neural precursor cell expressed, developmentally down-regulated 9 171 +/- 24 264 +/- 31 142 +/- 23 201 +/- 20 -0.016703 0.0430915 0.08859 0.0470127 0.0403083 NEK7 NIMA (never in mitosis gene a)-related kinase 7 2990 +/- 247 3754 +/- 214 2291 +/- 122 2919 +/- 48 -0.000365 0.0420786 0.002479 0.0245299 0.0114918 NETO2 neuropilin (NRP) and tolloid (TLL)-like 2 2261 +/- 64 1727 +/- 92 1797 +/- 68 1378 +/- 100 -1.76E-05 -0.001018 -0.009544 0.0059959 0.0224438 NEU4 sialidase 4 318 +/- 28 531 +/- 71 374 +/- 25 540 +/- 33 0.215951 0.0294723 0.003914 0.0028642 0.0682563 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive 3892 +/- 75 3653 +/- 71 3843 +/- 56 3902 +/- 34 0.309128 -0.043532 0.39059 0.0610563 0.3535764 NFIA nuclear factor I/A 731 +/- 78 982 +/- 55 989 +/- 53 935 +/- 121 0.147136 0.0267501 -0.697485 0.1030034 0.1772282 NFIC nuclear factor I/C (CCAAT-binding transcription factor) 198 +/- 10 244 +/- 11 275 +/- 22 264 +/- 14 0.015033 0.0111857 -0.689607 0.0046001 0.8942694 NFIL3 nuclear factor, interleukin 3 regulated 495 +/- 40 462 +/- 38 822 +/- 51 669 +/- 29 0.000782 -0.562878 -0.032292 0.5630724 0.1254024 NFKBIZ Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta 167 +/- 11 264 +/- 26 150 +/- 6 206 +/- 14 -0.035425 0.0112849 0.01217 0.0001388 0.0254422 NFX1 nuclear transcription factor, X-box binding 1 115 +/- 15 162 +/- 12 126 +/- 3 169 +/- 16 0.480669 0.0398384 0.047698 0.1197636 0.0784361 NHP2L1 NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) 59 +/- 14 219 +/- 15 65 +/- 8 167 +/- 16 -0.298555 1.605E-05 0.001334 8.789E-05 0.0458718 NIBP NIK and IKK{beta} binding protein 744 +/- 67 889 +/- 46 598 +/- 42 755 +/- 42 -0.019114 0.1096693 0.026365 0.160192 0.1644102 NIFUN NifU-like N-terminal domain containing 10519 +/- 373 10858 +/- 390 9520 +/- 257 10727 +/- 229 -0.01697 0.5434208 0.00664 0.7958581 0.0285253 NINJ2 ninjurin 2 672 +/- 49 990 +/- 158 361 +/- 35 494 +/- 25 -0.002231 0.1042603 0.014367 0.0107827 0.03445 NIPBL Nipped-B homolog (Drosophila) 651 +/- 32 814 +/- 41 781 +/- 26 815 +/- 32 0.035838 0.0111975 0.434143 0.0066467 0.6387933 NISCH nischarin 262 +/- 23 343 +/- 27 377 +/- 20 423 +/- 44 0.008463 0.0475203 0.38318 0.1719379 0.043231 NKD2 naked cuticle homolog 2 (Drosophila) 229 +/- 12 225 +/- 18 335 +/- 22 274 +/- 16 0.00102 -0.873328 -0.053989 0.951125 0.1893995 NKTR natural killer-tumor recognition sequence 456 +/- 55 637 +/- 40 647 +/- 63 788 +/- 65 0.000111 0.024757 0.155518 0.0520207 0.3418644 NME1 Non-metastatic cells 1, protein (NM23A) expressed in 1207 +/- 74 1482 +/- 34 1218 +/- 92 1399 +/- 47 -0.488309 0.011643 0.119899 0.1119294 0.3363699 NMNAT1 nicotinamide nucleotide adenylyltransferase 1 148 +/- 15 217 +/- 22 178 +/- 10 206 +/- 24 0.360594 0.0291658 0.321266 0.0339437 0.2288575 NMRAL1 NmrA-like family domain containing 1 1470 +/- 75 1003 +/- 79 1214 +/- 54 797 +/- 29 -0.000141 -0.00164 -0.000186 0.0303439 0.0085358 NMT2 N-myristoyltransferase 2 443 +/- 35 350 +/- 17 594 +/- 32 534 +/- 28 1.87E-05 -0.045269 -0.185807 0.0344018 0.2033577 NOG Noggin 599 +/- 81 458 +/- 67 733 +/- 50 542 +/- 33 0.051327 -0.208994 -0.011914 0.1772806 0.0994863 NOL8 nucleolar protein 8 1828 +/- 50 1420 +/- 66 1771 +/- 73 1225 +/- 62 -0.162685 -0.000763 -0.00031 0.0134244 0.0071962 NOP17 NOP17 882 +/- 62 1108 +/- 85 828 +/- 45 1014 +/- 32 -0.183839 0.0600401 0.00885 0.2589579 0.0290224 NOTUM notum pectinacetylesterase homolog (Drosophila) 87 +/- 16 107 +/- 11 136 +/- 15 92 +/- 6 0.274664 0.3216937 -0.036055 0.3311443 0.1019138 NPAS2 neuronal PAS domain protein 2 1237 +/- 69 1027 +/- 36 1162 +/- 50 1055 +/- 32 -0.492833 -0.028196 -0.104973 0.0059946 0.3813514 NPAT nuclear protein, ataxia-telangiectasia locus 490 +/- 15 619 +/- 32 435 +/- 15 535 +/- 41 -0.001088 0.0079427 0.071889 0.0227281 0.1146014

S1- alphabetical list of aging-related genes.xls Page 20 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG NPC1 Niemann-Pick disease, type C1 1631 +/- 195 2450 +/- 429 909 +/- 54 1244 +/- 72 -0.003597 0.1255651 0.006001 0.0150477 0.004993 NPEPL1 aminopeptidase-like 1 143 +/- 12 156 +/- 13 174 +/- 8 136 +/- 11 0.554359 0.4813468 -0.025601 0.7999007 0.1183292 NPHP3 nephronophthisis 3 (adolescent) 113 +/- 9 157 +/- 10 147 +/- 18 189 +/- 8 0.005721 0.0088437 0.07045 0.0690173 0.13618 NPL N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) 229 +/- 29 384 +/- 51 300 +/- 21 335 +/- 41 0.994805 0.0306947 0.479418 0.1158414 0.6960425 NPLOC4 nuclear protein localization 4 homolog (S. cerevisiae) 1165 +/- 45 1126 +/- 45 1102 +/- 47 968 +/- 34 -0.06142 -0.55794 -0.046812 0.6237396 0.1433947 NR2F1 Nuclear receptor subfamily 2, group F, member 1 3880 +/- 234 4227 +/- 278 4775 +/- 257 5717 +/- 210 0.001232 0.3628789 0.019733 0.6493127 0.043827 NR3C1 Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) 263 +/- 52 681 +/- 115 326 +/- 52 464 +/- 66 -0.266534 0.012777 0.138402 0.0010057 0.0254784 NRBP2 nuclear receptor binding protein 2 3166 +/- 354 3523 +/- 368 2980 +/- 110 3462 +/- 135 -0.457122 0.5009616 0.023662 0.355252 0.0119777 NRF1 nuclear respiratory factor 1 180 +/- 11 223 +/- 12 164 +/- 5 186 +/- 16 -0.006482 0.0251685 0.246261 0.0249377 0.1096532 NRP2 neuropilin 2 178 +/- 27 256 +/- 23 279 +/- 17 347 +/- 12 0.001843 0.0546684 0.010231 0.0441847 0.0569377 NRXN1 neurexin 1 10862 +/- 245 9499 +/- 402 10764 +/- 142 9886 +/- 287 0.770465 -0.019308 -0.03393 0.0314915 0.1487276 NSUN2 NOL1/NOP2/Sun domain family, member 2 521 +/- 29 653 +/- 50 633 +/- 51 677 +/- 39 0.005451 0.0534071 0.513679 0.2984479 0.8637154 NUBP1 nucleotide binding protein 1 (MinD homolog, E. coli) 679 +/- 25 548 +/- 12 607 +/- 21 506 +/- 19 -0.004407 -0.002201 -0.006862 0.0254274 0.0773208 NUDT10 nudix (nucleoside diphosphate linked moiety X)-type motif 10 282 +/- 18 221 +/- 15 266 +/- 20 231 +/- 22 -0.68097 -0.0254 -0.265813 0.0055507 0.163942 NUDT4 nudix (nucleoside diphosphate linked moiety X)-type motif 4 341 +/- 23 281 +/- 33 505 +/- 25 364 +/- 47 0.000121 -0.167955 -0.036215 0.2096351 0.049617 NUDT5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 884 +/- 75 1105 +/- 67 672 +/- 11 954 +/- 63 -0.003564 0.0533906 0.010161 0.0942105 0.1224147 NUFIP1 nuclear fragile X mental retardation protein interacting protein 1 389 +/- 16 344 +/- 13 423 +/- 26 378 +/- 24 0.012366 -0.053026 -0.23428 0.0239729 0.108255 NUFIP2 nuclear fragile X mental retardation protein interacting protein 2 761 +/- 34 710 +/- 36 845 +/- 39 746 +/- 16 0.029363 -0.328267 -0.051374 0.748716 0.0581558 NUP50 nucleoporin 50kDa 2247 +/- 143 3427 +/- 362 2766 +/- 251 3193 +/- 321 0.052566 0.0208199 0.325114 0.0368692 0.442796 NUP62 nucleoporin 62kDa 885 +/- 11 1158 +/- 43 1053 +/- 49 1241 +/- 35 0.01417 0.0009882 0.012691 0.0697167 0.041829 NUPL1 nucleoporin like 1 80 +/- 12 133 +/- 19 138 +/- 7 175 +/- 24 0.000357 0.0436761 0.212114 0.2357862 0.8285182 NUPL2 nucleoporin like 2 194 +/- 5 182 +/- 13 206 +/- 12 164 +/- 7 -0.987486 -0.45405 -0.015258 0.7668864 0.0074733 NUTF2 Nuclear transport factor 2 611 +/- 38 479 +/- 23 607 +/- 22 528 +/- 32 0.48148 -0.01707 -0.081001 0.022601 0.1385723 NVL nuclear VCP-like 300 +/- 30 444 +/- 43 292 +/- 21 362 +/- 6 -0.185107 0.0231641 0.018625 0.0013892 0.0526935 OBFC1 oligonucleotide/oligosaccharide-binding fold containing 1 224 +/- 12 220 +/- 22 169 +/- 11 211 +/- 12 -0.012381 -0.891075 0.028949 0.4253951 0.3264349 OCRL oculocerebrorenal syndrome of Lowe 5167 +/- 351 2651 +/- 414 4835 +/- 441 3145 +/- 483 0.924081 -0.000997 -0.030467 0.0289456 0.2772858 ODZ4 odz, odd Oz/ten-m homolog 4 (Drosophila) 1909 +/- 94 1463 +/- 108 1647 +/- 86 1434 +/- 117 -0.248314 -0.011219 -0.179929 0.0042511 0.0642511 OGDH oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1659 +/- 60 1455 +/- 67 1434 +/- 34 1400 +/- 37 -0.010026 -0.045988 -0.523984 0.0404441 0.3839513 OGFRL1 opioid growth factor receptor-like 1 121 +/- 12 168 +/- 17 211 +/- 16 189 +/- 23 0.006054 0.0544192 -0.447763 0.0564482 0.47636 OGG1 8-oxoguanine DNA glycosylase 301 +/- 28 351 +/- 24 298 +/- 15 364 +/- 22 0.700106 0.2095177 0.039468 0.3472369 0.0004648 OGN osteoglycin (osteoinductive factor, mimecan) 1651 +/- 630 839 +/- 341 397 +/- 58 709 +/- 94 -0.132398 -0.290932 0.029188 0.0859827 0.0012253 OGT O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide 1094 +/- 60 899 +/- 25 1800 +/- 83 1694 +/- 67 4.43E-07 -0.022121 -0.34876 0.1719069 0.6526878 OLIG1 oligodendrocyte transcription factor 1 10505 +/- 1321 11917 +/- 1508 6389 +/- 320 7664 +/- 455 -0.002276 0.4974144 0.053366 0.2657782 0.0402461 OLR1 oxidised low density lipoprotein (lectin-like) receptor 1 109 +/- 11 211 +/- 34 91 +/- 17 134 +/- 20 -0.097589 0.0274779 0.137991 0.000134 0.3977128 OPTN optineurin 1234 +/- 134 1443 +/- 56 1440 +/- 68 1677 +/- 80 0.000947 0.1953036 0.051872 0.2762996 0.044902 OR2C1 olfactory receptor, family 2, subfamily C, member 1 141 +/- 10 140 +/- 11 189 +/- 16 125 +/- 15 0.394261 -0.916289 -0.014564 0.8436501 0.0243241 ORC4L origin recognition complex, subunit 4-like (yeast) 941 +/- 34 812 +/- 27 846 +/- 20 797 +/- 54 -0.061326 -0.014334 -0.430875 0.0731478 0.5829527 OSBPL11 oxysterol binding protein-like 11 1537 +/- 69 1374 +/- 41 1376 +/- 35 1266 +/- 31 -0.011451 -0.075652 -0.044285 0.0694511 0.0146336 OSBPL1A oxysterol binding protein-like 1A 5228 +/- 458 5797 +/- 389 3578 +/- 80 4173 +/- 148 -0.000969 0.3665203 0.011533 0.3098806 0.0059963 OSBPL2 oxysterol binding protein-like 2 588 +/- 28 649 +/- 27 587 +/- 10 638 +/- 14 -0.745547 0.1550401 0.019473 0.0725911 0.0811676 OSBPL6 oxysterol binding protein-like 6 2412 +/- 155 1905 +/- 45 2468 +/- 135 1963 +/- 75 0.382613 -0.020642 -0.012145 0.0934375 0.2274816 OSGEP O-sialoglycoprotein endopeptidase 459 +/- 30 536 +/- 32 410 +/- 14 486 +/- 23 -0.0199 0.11213 0.027453 0.4425687 0.0759765 OSTalpha organic solute transporter alpha 185 +/- 40 66 +/- 8 151 +/- 24 54 +/- 13 -0.10308 -0.028624 -0.007414 0.0171587 0.046107 P2RX5 purinergic receptor P2X, ligand-gated ion channel, 5 1338 +/- 74 1083 +/- 93 1528 +/- 87 1238 +/- 63 0.025993 -0.058172 -0.025511 0.0974106 0.0695644

S1- alphabetical list of aging-related genes.xls Page 21 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG P2RY12 purinergic receptor P2Y, G-protein coupled, 12 1248 +/- 88 1177 +/- 79 1077 +/- 48 848 +/- 66 -0.011588 -0.565026 -0.023701 0.9972657 0.0388881 P8 p8 protein (candidate of metastasis 1) 397 +/- 62 578 +/- 55 378 +/- 56 493 +/- 36 -0.036934 0.0539708 0.12096 0.0367176 0.1038003 PA2G4 proliferation-associated 2G4, 38kDa 712 +/- 69 939 +/- 45 885 +/- 51 1182 +/- 44 0.000332 0.0231989 0.001752 0.0297561 0.0004348 PABPC1 Poly(A) binding protein, cytoplasmic 1 183 +/- 21 254 +/- 18 240 +/- 24 272 +/- 26 0.073825 0.0283996 0.40093 0.0113999 0.6629855 PABPC5 poly(A) binding protein, cytoplasmic 5 153 +/- 5 103 +/- 10 160 +/- 15 110 +/- 8 0.609611 -0.003193 -0.019985 0.089754 0.1251707 PACRG PARK2 co-regulated 1071 +/- 37 1394 +/- 73 893 +/- 44 1132 +/- 74 -0.000382 0.0049231 0.028089 0.0005899 0.0063267 PAIP1 poly(A) binding protein interacting protein 1 2718 +/- 182 2080 +/- 74 3154 +/- 104 2326 +/- 67 0.015668 -0.015324 -0.000136 0.031099 0.0141581 PAIP2 poly(A) binding protein interacting protein 2 6014 +/- 283 6884 +/- 216 5284 +/- 145 5886 +/- 240 -0.001414 0.0363882 0.070781 0.0951697 0.28479 PAK2 p21 (CDKN1A)-activated kinase 2 335 +/- 25 481 +/- 33 362 +/- 10 410 +/- 22 -0.468014 0.0059192 0.096547 0.0016975 0.5022974 PAK3 P21 (CDKN1A)-activated kinase 3 227 +/- 18 167 +/- 19 271 +/- 16 203 +/- 14 0.052076 -0.048202 -0.010497 0.0506195 0.0772543 PALLD palladin, cytoskeletal associated protein 483 +/- 53 658 +/- 48 529 +/- 39 581 +/- 31 -0.86 0.0351675 0.314434 0.0136144 0.5775141 PALMD palmdelphin 203 +/- 17 151 +/- 13 248 +/- 26 204 +/- 20 0.000514 -0.039876 -0.211303 0.202666 0.2398417 PAN3 PAN3 polyA specific ribonuclease subunit homolog (S. cerevisiae) 1456 +/- 117 1346 +/- 43 1368 +/- 61 1193 +/- 38 -0.214488 -0.411628 -0.040479 0.6692186 0.1739636 PAPD1 PAP associated domain containing 1 886 +/- 44 1400 +/- 87 1045 +/- 67 1422 +/- 80 0.027286 0.0009632 0.006476 0.002258 0.0023265 PAPOLG poly(A) polymerase gamma 791 +/- 23 698 +/- 22 821 +/- 24 839 +/- 22 0.005476 -0.014573 0.597447 0.041462 0.8523547 PAQR8 progestin and adipoQ receptor family member VIII 3877 +/- 236 4674 +/- 31 4024 +/- 144 4047 +/- 240 -0.556169 0.0191116 0.939482 0.1003113 0.3960777 PARP3 poly (ADP-ribose) polymerase family, member 3 333 +/- 39 495 +/- 52 342 +/- 27 363 +/- 17 -0.190787 0.0340818 0.517761 0.0298083 0.8354114 PARP8 poly (ADP-ribose) polymerase family, member 8 406 +/- 45 403 +/- 30 443 +/- 29 519 +/- 18 0.010472 -0.951028 0.053006 0.9421587 0.1955899 PASK PAS domain containing serine/threonine kinase 171 +/- 15 144 +/- 18 171 +/- 11 130 +/- 11 -0.845199 -0.279469 -0.029536 0.3171243 0.2230276 PB1 polybromo 1 2458 +/- 285 3086 +/- 101 2471 +/- 95 3125 +/- 70 0.913899 0.0817419 0.000415 0.137583 0.0063458 PBX1 Pre-B-cell leukemia transcription factor 1 1716 +/- 118 2769 +/- 217 1455 +/- 111 2238 +/- 91 -0.003412 0.0029746 0.000413 0.0044114 0.0061288 PCBP1 poly(rC) binding protein 1 3493 +/- 69 3140 +/- 89 3412 +/- 103 3322 +/- 114 0.975077 -0.011417 -0.572879 0.007885 0.4987079 PCDH1 protocadherin 1 (cadherin-like 1) 178 +/- 17 162 +/- 12 240 +/- 16 186 +/- 15 0.001299 -0.439022 -0.039922 0.1526281 0.0256257 PCDH10 protocadherin 10 956 +/- 148 1051 +/- 135 653 +/- 82 380 +/- 31 -0.000867 0.6474616 -0.019473 0.7187845 0.0700467 PCDHB14 protocadherin beta 14 224 +/- 13 229 +/- 9 342 +/- 16 269 +/- 6 0.001369 0.7321903 -0.004447 0.2135377 0.0333462 PCDHGB6 protocadherin gamma subfamily B, 6 4504 +/- 348 5671 +/- 569 2978 +/- 131 3473 +/- 103 -0.000517 0.1168144 0.016108 0.0748677 0.0446749 PCF11 PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) 929 +/- 93 1221 +/- 73 1180 +/- 136 1270 +/- 151 0.268297 0.0346984 0.669485 0.0934137 0.7682323 PCGF2 polycomb group ring finger 2 253 +/- 17 158 +/- 15 362 +/- 10 234 +/- 26 2.51E-05 -0.001817 -0.005282 0.0438859 0.0396435 PCGF5 polycomb group ring finger 5 241 +/- 31 382 +/- 71 220 +/- 18 310 +/- 16 -0.126114 0.1128983 0.004382 0.024573 0.016707 PCID2 PCI domain containing 2 422 +/- 26 371 +/- 24 406 +/- 11 289 +/- 11 -0.037605 -0.183262 -4.09E-05 0.0833054 0.0063696 PCNP PEST proteolytic signal containing nuclear protein 4478 +/- 358 5960 +/- 334 4068 +/- 199 4741 +/- 246 -0.010461 0.0127892 0.065491 0.0062998 0.2290232 PCOLN3 procollagen (type III) N-endopeptidase 961 +/- 26 1244 +/- 123 979 +/- 64 1383 +/- 101 0.252081 0.069583 0.012167 0.2008688 0.147378 PCSK2 proprotein convertase subtilisin/kexin type 2 3146 +/- 220 2328 +/- 198 2601 +/- 119 2049 +/- 105 -0.013546 -0.020418 -0.006951 0.0123245 0.060491 PCTK2 PCTAIRE protein kinase 2 4630 +/- 476 3780 +/- 295 5133 +/- 337 4095 +/- 92 0.204156 -0.166031 -0.02624 0.2028421 0.1347022 PDCD6IP programmed cell death 6 interacting protein 1108 +/- 45 997 +/- 36 1209 +/- 22 1087 +/- 41 0.005832 -0.084134 -0.03722 0.1395256 0.0535386 PDDC1 Parkinson disease 7 domain containing 1 828 +/- 44 710 +/- 44 853 +/- 30 700 +/- 27 0.635195 -0.084896 -0.003948 0.1653166 0.0069087 PDE8B phosphodiesterase 8B 248 +/- 54 368 +/- 71 377 +/- 54 570 +/- 63 0.005075 0.2073609 0.04528 0.6610919 0.3982629 PDGFA platelet-derived growth factor alpha polypeptide 71 +/- 16 134 +/- 20 87 +/- 13 170 +/- 42 0.29246 0.0358641 0.119292 0.1336643 0.0960993 PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homo 468 +/- 21 394 +/- 23 454 +/- 37 444 +/- 37 0.70054 -0.042901 -0.846961 0.0993042 0.6062047 PDIA2 protein disulfide isomerase family A, member 2 137 +/- 34 221 +/- 20 178 +/- 12 237 +/- 20 0.237967 0.0633597 0.044121 0.1151384 0.0657758 PDIA4 protein disulfide isomerase family A, member 4 1106 +/- 38 1038 +/- 64 1073 +/- 53 906 +/- 43 -0.218147 -0.387693 -0.038213 0.5783893 0.0289826 PDK3 Pyruvate dehydrogenase kinase, isozyme 3 704 +/- 98 960 +/- 111 993 +/- 129 1593 +/- 94 0.000496 0.113882 0.00477 0.4282027 0.0643055 PDLIM4 PDZ and LIM domain 4 397 +/- 29 502 +/- 35 398 +/- 27 477 +/- 30 -0.430923 0.0456115 0.083153 0.0597891 0.2987527 PDXP pyridoxal (pyridoxine, vitamin B6) phosphatase 5584 +/- 267 4404 +/- 240 5656 +/- 156 4968 +/- 141 0.186091 -0.00839 -0.009631 0.0004247 0.0147433

S1- alphabetical list of aging-related genes.xls Page 22 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG PDZRN4 PDZ domain containing RING finger 4 961 +/- 82 934 +/- 58 831 +/- 30 732 +/- 22 -0.021366 -0.790399 -0.028345 0.5257061 0.0958787 PEA15 phosphoprotein enriched in astrocytes 15 14228 +/- 407 15719 +/- 171 16261 +/- 383 17626 +/- 635 0.001449 0.0125159 0.110018 0.0156896 0.3490645 PEG10 paternally expressed 10 1103 +/- 193 1186 +/- 106 1593 +/- 124 1132 +/- 65 0.264642 0.7157906 -0.012088 0.7585418 0.0534317 PEX11A Peroxisomal biogenesis factor 11A 115 +/- 9 142 +/- 7 134 +/- 13 144 +/- 10 0.222655 0.0408075 0.54528 0.0214573 0.610458 PEX14 peroxisomal biogenesis factor 14 1046 +/- 74 925 +/- 41 1172 +/- 79 945 +/- 26 0.257463 -0.190372 -0.034155 0.0849895 0.0661495 PEX6 peroxisomal biogenesis factor 6 1024 +/- 62 855 +/- 43 1081 +/- 68 886 +/- 19 0.262729 -0.051379 -0.033672 0.0169983 0.0531548 PFAAP5 phosphonoformate immuno-associated protein 5 540 +/- 18 663 +/- 31 644 +/- 46 682 +/- 23 0.08712 0.0093525 0.484303 0.0360972 0.9992894 PFDN1 prefoldin subunit 1 3757 +/- 69 3495 +/- 74 3517 +/- 72 3281 +/- 42 -0.02002 -0.026758 -0.022375 0.003462 0.1034135 PFDN4 prefoldin subunit 4 737 +/- 71 858 +/- 41 809 +/- 83 1017 +/- 19 0.014219 0.178896 0.053249 0.1720676 0.2405397 PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 531 +/- 38 759 +/- 35 440 +/- 25 533 +/- 18 -0.00079 0.0013335 0.014641 0.005562 0.0135006 PFKL phosphofructokinase, liver 1784 +/- 148 1890 +/- 147 1627 +/- 63 1907 +/- 88 -0.861285 0.6198749 0.033723 0.8964558 0.1126362 PGAM1 phosphoglycerate mutase 1 (brain) 11785 +/- 417 13568 +/- 474 10224 +/- 320 12402 +/- 471 -0.001479 0.0183042 0.006018 0.1469321 0.0390915 PGAM2 phosphoglycerate mutase 2 (muscle) 175 +/- 19 219 +/- 19 163 +/- 19 222 +/- 6 -0.945968 0.1251703 0.024152 0.1214422 0.0491783 PGM2L1 phosphoglucomutase 2-like 1 4422 +/- 615 3043 +/- 130 4291 +/- 170 3223 +/- 103 0.986368 -0.07524 -0.000674 0.0376398 0.0035047 PHC3 Polyhomeotic like 3 (Drosophila) 83 +/- 5 113 +/- 9 127 +/- 13 127 +/- 17 0.036711 0.0254323 -0.986452 0.0747679 0.4313701 PHF10 PHD finger protein 10 327 +/- 31 419 +/- 15 380 +/- 14 460 +/- 26 0.044383 0.0325161 0.034908 0.0388483 0.1085417 PHF20 PHD finger protein 20 592 +/- 14 520 +/- 25 526 +/- 18 503 +/- 18 -0.093536 -0.036912 -0.388661 0.1862259 0.8539314 PHF21A PHD finger protein 21A 468 +/- 21 547 +/- 29 481 +/- 21 523 +/- 41 -0.946357 0.0494199 0.396797 0.0360878 0.4265687 PHF3 PHD finger protein 3 1737 +/- 78 2049 +/- 109 1967 +/- 66 2013 +/- 122 0.257221 0.04437 0.752339 0.0019306 0.2314451 PHLDA1 pleckstrin homology-like domain, family A, member 1 421 +/- 36 319 +/- 24 453 +/- 52 320 +/- 63 0.712182 -0.043072 -0.140808 0.014175 0.1210356 PHOSPHOphosphatase, orphan 2 671 +/- 78 1066 +/- 59 648 +/- 36 1124 +/- 54 0.761082 0.0026659 0.000135 0.0303631 0.0066343 PIGB phosphatidylinositol glycan, class B 477 +/- 19 647 +/- 44 496 +/- 15 519 +/- 26 -0.178267 0.0099728 0.456551 0.0010067 0.5582237 PIGG phosphatidylinositol glycan, class G 748 +/- 78 456 +/- 62 758 +/- 104 521 +/- 43 0.535688 -0.015859 -0.075387 0.0958849 0.2558164 PIGS phosphatidylinositol glycan, class S 2823 +/- 73 3263 +/- 183 2696 +/- 71 3307 +/- 125 -0.826099 0.062593 0.004496 0.2144759 0.0931655 PIGZ phosphatidylinositol glycan, class Z 644 +/- 33 916 +/- 90 686 +/- 41 941 +/- 35 0.263631 0.0274333 0.001051 0.094047 0.0159916 PIK3C2A Phosphoinositide-3-kinase, class 2, alpha polypeptide 1130 +/- 44 1263 +/- 40 1085 +/- 33 1109 +/- 30 -0.003738 0.0481159 0.603829 0.0485674 0.7559619 PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta) 5364 +/- 460 3833 +/- 348 4458 +/- 93 3892 +/- 156 -0.338919 -0.025608 -0.017781 0.0210901 0.0952849 PINK1 PTEN induced putative kinase 1 5970 +/- 290 3813 +/- 313 5732 +/- 418 4288 +/- 150 0.726644 -0.000504 -0.016544 0.0050779 0.1139928 PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type I, beta 1195 +/- 134 787 +/- 70 608 +/- 59 541 +/- 13 -0.001939 -0.028873 -0.314437 0.0181839 0.2623352 PIP5K3 phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III 1685 +/- 66 1442 +/- 57 1387 +/- 56 1232 +/- 71 -0.000187 -0.020366 -0.126116 0.1793597 0.222708 PIPOX pipecolic acid oxidase 388 +/- 35 493 +/- 32 447 +/- 29 523 +/- 16 0.046104 0.0521533 0.051334 0.0365581 0.3326665 PITPNC1 Phosphatidylinositol transfer protein, cytoplasmic 1 130 +/- 18 150 +/- 13 145 +/- 6 207 +/- 21 0.04088 0.3841422 0.036598 0.1265199 0.0304022 PKIA Protein kinase (cAMP-dependent, catalytic) inhibitor alpha 252 +/- 9 223 +/- 13 277 +/- 14 229 +/- 14 0.102118 -0.094144 -0.041148 0.5273853 0.1715247 PKN1 protein kinase N1 1025 +/- 87 1327 +/- 101 952 +/- 42 1034 +/- 60 -0.051585 0.0479771 0.299131 0.1114371 0.5076743 PKP4 plakophilin 4 192 +/- 40 356 +/- 46 228 +/- 41 321 +/- 34 0.802794 0.0232074 0.116215 0.1495779 0.0667115 PLA2G12Aphospholipase A2, group XIIA 659 +/- 57 425 +/- 65 583 +/- 43 397 +/- 74 -0.059816 -0.021907 -0.068933 0.0042712 0.0052474 PLA2G4C phospholipase A2, group IVC (cytosolic, calcium-independent) 321 +/- 37 368 +/- 21 312 +/- 19 375 +/- 20 -0.774809 0.3108117 0.048104 0.8041481 0.0485503 PLAA phospholipase A2-activating protein 952 +/- 29 788 +/- 29 921 +/- 43 883 +/- 20 0.539691 -0.002377 -0.443566 0.010247 0.8711376 PLAC9 placenta-specific 9 1835 +/- 282 1130 +/- 254 1524 +/- 212 916 +/- 172 -0.023748 -0.093037 -0.053552 0.4320796 0.3136659 PLAGL2 pleiomorphic adenoma gene-like 2 189 +/- 12 240 +/- 12 225 +/- 13 207 +/- 23 0.772152 0.013757 -0.511907 0.0128052 0.6163932 PLCH1 phospholipase C, eta 1 327 +/- 12 405 +/- 32 217 +/- 23 304 +/- 19 -3.84E-05 0.0581358 0.016981 0.1075869 0.099588 PLCH2 phospholipase C, eta 2 370 +/- 51 502 +/- 14 513 +/- 63 801 +/- 59 0.000977 0.0493627 0.008957 0.0679472 0.0876322 PLCXD2 Phosphatidylinositol-specific phospholipase C, X domain containing 2 4569 +/- 505 3022 +/- 283 4069 +/- 225 3379 +/- 431 -0.783292 -0.028738 -0.20446 0.0259544 0.4051163 PLD2 phospholipase D2 201 +/- 20 312 +/- 13 231 +/- 27 280 +/- 18 -0.805722 0.0013884 0.165643 0.0072535 0.2020121

S1- alphabetical list of aging-related genes.xls Page 23 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG PLEK pleckstrin 301 +/- 28 251 +/- 18 267 +/- 22 206 +/- 13 -0.133986 -0.167953 -0.044632 0.3739507 0.1348917 PLEKHA1 pleckstrin homology domain containing, family A (phosphoinositide binding specific) membe 1885 +/- 104 2268 +/- 98 1949 +/- 90 1867 +/- 107 -0.243407 0.0234509 -0.575564 0.0477602 0.8494424 PLEKHA5 Pleckstrin homology domain containing, family A member 5 566 +/- 51 398 +/- 55 952 +/- 56 599 +/- 55 0.000798 -0.048983 -0.001487 0.0944605 0.0453485 PLEKHA6 pleckstrin homology domain containing, family A member 6 566 +/- 46 738 +/- 53 585 +/- 29 732 +/- 40 0.443461 0.0342898 0.018921 0.1626649 0.080752 PLEKHB1 pleckstrin homology domain containing, family B (evectins) member 1 6842 +/- 885 9643 +/- 1345 4705 +/- 239 6453 +/- 287 -0.01047 0.1174381 0.001452 0.0255133 0.0059289 PLEKHG6 pleckstrin homology domain containing, family G (with RhoGef domain) member 6 368 +/- 21 360 +/- 55 442 +/- 17 355 +/- 17 0.442256 -0.889862 -0.006192 0.8010314 0.2480882 PLEKHH1 pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 5498 +/- 891 7056 +/- 1244 2329 +/- 184 3044 +/- 221 -0.001295 0.3350938 0.037095 0.1269824 0.055862 PLK1 polo-like kinase 1 (Drosophila) 21403 +/- 877 23680 +/- 475 19863 +/- 1093 22695 +/- 710 -0.047931 0.0530814 0.060486 0.3501264 0.3189812 PLK2 polo-like kinase 2 (Drosophila) 4576 +/- 519 3141 +/- 223 3989 +/- 267 3502 +/- 175 -0.793838 -0.039743 -0.163947 0.0759949 0.7166391 PLSCR4 phospholipid scramblase 4 292 +/- 36 311 +/- 25 313 +/- 18 251 +/- 17 -0.832147 0.6731242 -0.032128 0.341487 0.0208081 PLTP phospholipid transfer protein 1127 +/- 38 1268 +/- 54 1124 +/- 55 1435 +/- 28 0.098579 0.0620181 0.001337 0.0178421 0.0316474 PLXNA2 Plexin A2 261 +/- 24 167 +/- 18 177 +/- 13 194 +/- 14 -0.334845 -0.011535 0.392874 0.0942256 0.6578159 PLXNA3 plexin A3 205 +/- 22 318 +/- 36 319 +/- 36 416 +/- 24 0.017336 0.0281193 0.054836 0.0386071 0.213522 PLXNB3 plexin B3 457 +/- 56 453 +/- 43 384 +/- 19 316 +/- 15 -0.015855 -0.953366 -0.019454 0.6546227 0.0750267 PMS2 PMS2 postmeiotic segregation increased 2 (S. cerevisiae) 429 +/- 49 1254 +/- 258 530 +/- 58 1843 +/- 341 0.028227 0.0231495 0.017205 0.2009082 0.1194689 PNCK pregnancy upregulated non-ubiquitously expressed CaM kinase 583 +/- 36 459 +/- 43 769 +/- 37 629 +/- 37 0.000565 -0.052096 -0.026386 0.0213662 0.0186213 PNLDC1 poly(A)-specific ribonuclease (PARN)-like domain containing 1 203 +/- 62 42 +/- 1 212 +/- 57 60 +/- 6 0.271555 -0.047861 -0.044612 0.1958038 0.1312138 PNMA1 paraneoplastic antigen MA1 10959 +/- 391 9451 +/- 261 10858 +/- 318 10579 +/- 360 0.288548 -0.011211 -0.577071 0.0056188 0.4872609 PNN pinin, desmosome associated protein 870 +/- 34 1067 +/- 32 937 +/- 58 1028 +/- 61 0.875786 0.0019071 0.307626 0.0385508 0.8431402 POLD2 Polymerase (DNA directed), delta 2, regulatory subunit 50kDa 88 +/- 13 142 +/- 12 136 +/- 20 197 +/- 12 0.002295 0.0118311 0.028238 0.0796663 0.2561202 POLDIP2 polymerase (DNA-directed), delta interacting protein 2 1060 +/- 90 803 +/- 50 1016 +/- 25 1023 +/- 36 0.348473 -0.039051 0.87296 0.0189631 0.6370655 POLE4 polymerase (DNA-directed), epsilon 4 (p12 subunit) 2611 +/- 103 2205 +/- 69 2073 +/- 57 2027 +/- 37 -0.004837 -0.009911 -0.52405 0.0030335 0.945593 POLG polymerase (DNA directed), gamma 1237 +/- 137 1440 +/- 127 1489 +/- 88 1711 +/- 27 0.01403 0.3033735 0.053158 0.1883551 0.1166869 POLR2G polymerase (RNA) II (DNA directed) polypeptide G 1501 +/- 46 1883 +/- 158 1045 +/- 54 1288 +/- 36 -0.000348 0.0603422 0.005367 0.0213566 0.0389687 POLR2J polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa 2255 +/- 115 1934 +/- 54 2042 +/- 31 1942 +/- 47 -0.214424 -0.039377 -0.118458 0.0652443 0.1761409 PON2 paraoxonase 2 3263 +/- 296 4425 +/- 150 3317 +/- 229 3671 +/- 180 -0.11293 0.0090922 0.255642 0.0131171 0.5617798 PPAP2B phosphatidic acid phosphatase type 2B 2720 +/- 167 3718 +/- 213 2634 +/- 127 2728 +/- 239 -0.040275 0.0045712 0.74131 0.0631569 0.390909 PPARG Peroxisome proliferative activated receptor, gamma 118 +/- 5 118 +/- 16 156 +/- 13 117 +/- 8 0.11829 -0.977405 -0.035008 0.8395201 0.0221271 PPIA peptidylprolyl isomerase A (cyclophilin A) 281 +/- 45 463 +/- 32 349 +/- 57 440 +/- 15 0.185757 0.0089627 0.174852 0.0153725 0.261195 PPID peptidylprolyl isomerase D (cyclophilin D) 303 +/- 34 207 +/- 13 325 +/- 26 339 +/- 63 0.069387 -0.034976 0.847746 0.0315415 0.2788419 PPM1B protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform 2968 +/- 253 3629 +/- 258 2741 +/- 80 2996 +/- 47 -0.046699 0.0972619 0.02543 0.0367724 0.0098338 PPM1E protein phosphatase 1E (PP2C domain containing) 2356 +/- 357 1782 +/- 252 2989 +/- 122 2473 +/- 54 0.006709 -0.221718 -0.0065 0.2717452 0.074406 PPP1R3E Protein phosphatase 1, regulatory (inhibitor) subunit 3E 694 +/- 57 902 +/- 71 992 +/- 47 1125 +/- 45 0.000582 0.0457328 0.071171 0.0148811 0.0623085 PPP2R5C protein phosphatase 2, regulatory subunit B (B56), gamma isoform 1380 +/- 94 967 +/- 75 1220 +/- 33 860 +/- 51 -0.02482 -0.006726 -0.000553 0.1080542 0.0232061 PPP4R2 protein phosphatase 4, regulatory subunit 2 886 +/- 44 1162 +/- 105 1013 +/- 60 1050 +/- 98 0.899093 0.0471725 0.759742 0.0002986 0.3021204 PPWD1 peptidylprolyl isomerase domain and WD repeat containing 1 248 +/- 10 294 +/- 14 268 +/- 8 252 +/- 15 -0.678991 0.0226652 -0.389625 0.0013421 0.9955112 PQLC3 PQ loop repeat containing 3 422 +/- 15 400 +/- 47 254 +/- 11 298 +/- 12 -0.000134 -0.674626 0.023289 0.696103 0.3334257 PR47 platelet receptor for type III collagen, 47 kDa 322 +/- 22 435 +/- 46 397 +/- 25 512 +/- 42 0.002998 0.0619977 0.054506 0.0131489 0.0095289 PRDM2 PR domain containing 2, with ZNF domain 347 +/- 45 386 +/- 34 436 +/- 22 557 +/- 23 0.001651 0.5105142 0.004008 0.5401439 0.004555 PRDX5 peroxiredoxin 5 3941 +/- 95 3412 +/- 66 3326 +/- 71 3314 +/- 37 -0.008912 -0.0014 -0.885484 0.0020949 0.9175833 PRELP proline/arginine-rich end leucine-rich repeat protein 1546 +/- 52 1157 +/- 104 1515 +/- 84 1157 +/- 70 -0.685069 -0.011629 -0.009821 0.0140331 0.0041108 PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit 294 +/- 22 397 +/- 21 287 +/- 16 334 +/- 20 -0.073036 0.0066895 0.103806 0.0041265 0.0405637 PRKAB2 protein kinase, AMP-activated, beta 2 non-catalytic subunit 697 +/- 64 885 +/- 68 772 +/- 23 952 +/- 40 0.079533 0.0722526 0.006777 0.2516626 0.1004645 PRKCH protein kinase C, eta 181 +/- 18 255 +/- 18 129 +/- 12 131 +/- 19 -0.000728 0.0168632 0.943452 0.1302874 0.8546068

S1- alphabetical list of aging-related genes.xls Page 24 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG PRKRIR protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of ( 1194 +/- 105 1357 +/- 44 1231 +/- 45 1098 +/- 38 -0.177295 0.196904 -0.049313 0.2622106 0.112416 PRNPIP prion protein interacting protein 2458 +/- 123 2089 +/- 74 2388 +/- 39 2292 +/- 67 0.600677 -0.032218 -0.26005 0.0264134 0.1030597 PRO0149 PRO0149 protein 3917 +/- 388 3006 +/- 191 4784 +/- 216 3768 +/- 160 0.000998 -0.071798 -0.004643 0.1491221 0.0692187 PROCA1 proline-rich cyclin A1-interacting protein 188 +/- 17 132 +/- 6 195 +/- 11 162 +/- 7 0.097641 -0.019045 -0.031181 0.1049325 0.2584503 PROS1 protein S (alpha) 774 +/- 108 426 +/- 28 473 +/- 63 375 +/- 23 -0.044059 -0.022169 -0.190453 0.0335408 0.2462449 PRPF39 PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae) 398 +/- 54 361 +/- 33 488 +/- 33 340 +/- 41 0.273474 -0.57176 -0.023066 0.945514 0.0178668 PRPS2 phosphoribosyl pyrophosphate synthetase 2 1966 +/- 106 1443 +/- 29 1604 +/- 58 1369 +/- 46 -0.02071 -0.003525 -0.011978 0.0015139 0.084104 PRR13 proline rich 13 3251 +/- 117 3856 +/- 164 2723 +/- 24 3074 +/- 150 -0.000652 0.0147229 0.079234 0.0860497 0.1849899 PRRG1 proline rich Gla (G-carboxyglutamic acid) 1 909 +/- 109 1222 +/- 166 601 +/- 15 713 +/- 29 -0.004497 0.149903 0.01455 0.0265939 0.0612018 PRRX2 paired related homeobox 2 228 +/- 13 276 +/- 7 263 +/- 27 273 +/- 24 0.431085 0.0126135 0.791143 0.1193473 0.7892399 PRSS21 protease, serine, 21 (testisin) 154 +/- 18 182 +/- 13 167 +/- 4 246 +/- 21 0.020264 0.2276938 0.019111 0.026258 2.454E-05 PRSS22 protease, serine, 22 376 +/- 33 245 +/- 15 364 +/- 29 215 +/- 21 -0.385129 -0.009084 -0.002358 0.0469894 0.0596132 PSCD4 pleckstrin homology, Sec7 and coiled-coil domains 4 213 +/- 21 291 +/- 13 199 +/- 18 202 +/- 16 -0.035829 0.012288 0.924077 0.0949477 0.4844499 PSMA3 proteasome (prosome, macropain) subunit, alpha type, 3 1537 +/- 38 1871 +/- 67 1481 +/- 61 1913 +/- 144 -0.654743 0.0025823 0.036339 0.0475916 0.1628958 PSMA6 proteasome (prosome, macropain) subunit, alpha type, 6 5154 +/- 216 4141 +/- 221 4713 +/- 143 4252 +/- 182 -0.356986 -0.008318 -0.081582 0.1311745 0.0202982 PSMB4 proteasome (prosome, macropain) subunit, beta type, 4 3705 +/- 115 3631 +/- 124 3280 +/- 73 3573 +/- 83 -0.020303 -0.669068 0.027907 0.9954379 0.0416578 PSMB7 Proteasome (prosome, macropain) subunit, beta type, 7 371 +/- 27 515 +/- 39 467 +/- 43 650 +/- 85 0.007924 0.0143763 0.103113 0.0689831 0.4534666 PSMB9 proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) 262 +/- 10 349 +/- 12 233 +/- 6 295 +/- 8 -0.001327 0.0002752 0.000367 0.0088962 0.033051 PSMC5 proteasome (prosome, macropain) 26S subunit, ATPase, 5 4810 +/- 47 5639 +/- 234 4899 +/- 178 5254 +/- 108 -0.344103 0.015609 0.125388 0.1834994 0.3064435 PSMD11 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 1622 +/- 35 1788 +/- 38 1666 +/- 29 1742 +/- 29 -0.96161 0.0092493 0.094643 0.0050109 0.225815 PSMD4 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 2476 +/- 113 2897 +/- 73 2016 +/- 75 2132 +/- 95 -1.49E-05 0.0127068 0.364098 0.0997047 0.5086628 PSME2 proteasome (prosome, macropain) activator subunit 2 (PA28 beta) 821 +/- 80 977 +/- 77 784 +/- 43 921 +/- 39 -0.696512 0.1883069 0.042435 0.3134244 0.2395558 PSME3 proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) 1539 +/- 66 1271 +/- 42 1431 +/- 65 1338 +/- 73 -0.704044 -0.008494 -0.364471 0.0033565 0.4156583 PSPC1 paraspeckle component 1 4137 +/- 535 3110 +/- 267 3353 +/- 193 2357 +/- 289 -0.016884 -0.127704 -0.023391 0.5110806 0.1583018 PTBP2 polypyrimidine tract binding protein 2 2307 +/- 103 1782 +/- 161 2298 +/- 39 1811 +/- 152 0.989039 -0.023819 -0.03083 0.4386139 0.2161026 PTDSS2 phosphatidylserine synthase 2 563 +/- 47 649 +/- 35 490 +/- 18 620 +/- 48 -0.098639 0.1791052 0.049207 0.2676431 0.0150726 PTHB1 parathyroid hormone-responsive B1 158 +/- 16 111 +/- 9 140 +/- 6 113 +/- 12 -0.416564 -0.033637 -0.08911 0.0305044 0.0151372 PTK2 PTK2 protein tyrosine kinase 2 352 +/- 24 487 +/- 29 419 +/- 31 528 +/- 44 0.091486 0.005345 0.081022 0.0593681 0.1412482 PTMS parathymosin 564 +/- 27 451 +/- 59 772 +/- 43 611 +/- 28 0.001747 -0.12446 -0.013133 0.1036381 0.0200266 PTP4A2 protein tyrosine phosphatase type IVA, member 2 6286 +/- 287 7210 +/- 518 5634 +/- 234 7240 +/- 434 -0.388109 0.1580916 0.016418 0.0715423 0.1463042 PTP4A3 protein tyrosine phosphatase type IVA, member 3 107 +/- 7 143 +/- 10 103 +/- 8 139 +/- 10 -0.497831 0.0132158 0.023196 0.1971961 0.0717171 PTPLAD2 protein tyrosine phosphatase-like A domain containing 2 114 +/- 22 191 +/- 25 74 +/- 9 99 +/- 11 -0.003222 0.0418422 0.112482 0.0235631 0.4311144 PTPN21 Protein tyrosine phosphatase, non-receptor type 21 156 +/- 37 305 +/- 48 153 +/- 15 276 +/- 31 -0.174366 0.036046 0.013349 0.1043827 0.0182375 PTPRB protein tyrosine phosphatase, receptor type, B 193 +/- 17 229 +/- 15 142 +/- 9 176 +/- 12 -0.000313 0.1467456 0.053789 0.1643753 0.1592827 PTPRN2 protein tyrosine phosphatase, receptor type, N polypeptide 2 6494 +/- 194 5488 +/- 322 6194 +/- 400 5533 +/- 188 -0.566377 -0.027432 -0.178053 0.0014077 0.3132724 PUM2 pumilio homolog 2 (Drosophila) 2633 +/- 77 1935 +/- 111 2753 +/- 131 1997 +/- 141 0.411145 -0.000604 -0.003648 0.0556231 0.0816452 PVALB parvalbumin 391 +/- 24 469 +/- 50 287 +/- 22 418 +/- 28 -0.020259 0.1995472 0.005842 0.5771577 0.0334362 PVRL1 poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin) 255 +/- 18 185 +/- 22 246 +/- 25 246 +/- 12 0.393693 -0.032584 0.995348 0.0722514 0.7996456 PYCR1 pyrroline-5-carboxylate reductase 1 547 +/- 59 391 +/- 22 506 +/- 30 365 +/- 21 -0.39905 -0.045017 -0.004592 0.0374613 0.0463616 QSCN6L1 quiescin Q6-like 1 715 +/- 62 579 +/- 58 1381 +/- 87 1148 +/- 33 1.21E-09 -0.143148 -0.044212 0.175206 0.1498206 QTRT1 queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) 256 +/- 22 498 +/- 21 323 +/- 31 587 +/- 36 0.020811 1.482E-05 0.000476 0.0059661 0.0016492 RAB12 RAB12, member RAS oncogene family 321 +/- 25 439 +/- 34 442 +/- 78 498 +/- 58 0.034932 0.0211394 0.581549 0.0528651 0.6551118 RAB21 RAB21, member RAS oncogene family 670 +/- 41 691 +/- 68 764 +/- 30 643 +/- 32 0.677724 0.800378 -0.022052 0.2947003 0.0113321 RAB28 RAB28, member RAS oncogene family 757 +/- 67 1355 +/- 52 860 +/- 38 1404 +/- 71 0.110907 4.717E-05 0.000444 0.0053232 0.0093864

S1- alphabetical list of aging-related genes.xls Page 25 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG RAB32 RAB32, member RAS oncogene family 198 +/- 18 242 +/- 23 137 +/- 8 201 +/- 18 -0.000716 0.1654716 0.020572 0.1703733 0.1214088 RAB35 RAB35, member RAS oncogene family 778 +/- 29 811 +/- 19 718 +/- 14 670 +/- 5 -0.001143 0.3674905 -0.015566 0.5906463 0.0883262 RAB3GAP2RAB3 GTPase activating protein subunit 2 (non-catalytic) 2868 +/- 148 3105 +/- 104 2399 +/- 77 2721 +/- 85 -0.000532 0.2241835 0.020968 0.4857475 0.0543959 RAB6B RAB6B, member RAS oncogene family 1509 +/- 149 954 +/- 120 1616 +/- 206 1444 +/- 168 0.170469 -0.016509 -0.532763 0.0119622 0.5249062 RAB7 RAB7, member RAS oncogene family 9341 +/- 493 9851 +/- 264 7242 +/- 277 8105 +/- 249 -1.27E-05 0.3893238 0.045658 0.7249005 0.2878582 RAB8A RAB8A, member RAS oncogene family 660 +/- 25 807 +/- 25 552 +/- 11 656 +/- 19 -6.22E-05 0.0021892 0.002421 0.0048684 0.0003511 RABGAP1 RAB GTPase activating protein 1 116 +/- 13 189 +/- 11 144 +/- 11 164 +/- 8 0.560861 0.0016235 0.157811 0.0074506 0.1397332 RABL4 RAB, member of RAS oncogene family-like 4 740 +/- 95 1128 +/- 110 549 +/- 56 835 +/- 106 -0.000823 0.0238958 0.054058 0.0768268 0.3212915 RAC1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) 4505 +/- 188 3402 +/- 520 4425 +/- 250 3002 +/- 507 -0.648917 -0.091015 -0.045985 0.1407843 0.0506256 RAD50 RAD50 homolog (S. cerevisiae) 416 +/- 27 289 +/- 24 390 +/- 21 293 +/- 18 -0.34837 -0.005346 -0.006816 0.0174557 0.0062243 RAE1 RAE1 RNA export 1 homolog (S. pombe) 1175 +/- 43 1040 +/- 42 1125 +/- 36 1035 +/- 26 -0.368441 -0.048286 -0.073808 0.063973 0.3403386 RALGPS2 Ral GEF with PH domain and SH3 binding motif 2 280 +/- 27 193 +/- 19 326 +/- 37 295 +/- 16 0.008765 -0.026246 -0.484099 0.2424974 0.6605985 RANBP5 RAN binding protein 5 936 +/- 51 1233 +/- 94 1110 +/- 54 1359 +/- 44 0.039116 0.025319 0.005956 0.1892396 0.1865095 RANBP6 RAN binding protein 6 782 +/- 58 993 +/- 47 796 +/- 88 1061 +/- 36 0.631819 0.0186024 0.02899 0.0471226 0.0747294 RANGNRF RAN guanine nucleotide release factor 425 +/- 34 318 +/- 15 318 +/- 17 272 +/- 27 -0.003175 -0.024131 -0.192118 0.0152892 0.0205336 RAP80 receptor associated protein 80 1079 +/- 80 1269 +/- 127 891 +/- 21 956 +/- 15 -0.005113 0.2402403 0.033389 0.3779647 0.1303282 RAPGEF1 Rap guanine nucleotide exchange factor (GEF) 1 616 +/- 50 396 +/- 101 775 +/- 26 416 +/- 82 0.043348 -0.091584 -0.0095 0.072241 0.0039693 RASA3 RAS p21 protein activator 3 162 +/- 27 166 +/- 20 200 +/- 15 257 +/- 20 0.008713 0.9114708 0.052284 0.8869965 0.0237445 RASGRF1 Ras protein-specific guanine nucleotide-releasing factor 1 125 +/- 16 76 +/- 11 145 +/- 12 104 +/- 10 0.077015 -0.031471 -0.025192 0.0752486 0.0253698 RASGRF2 Ras protein-specific guanine nucleotide-releasing factor 2 335 +/- 22 273 +/- 17 423 +/- 53 365 +/- 12 0.015254 -0.048142 -0.333609 0.0328393 0.3533591 RBBP6 retinoblastoma binding protein 6 272 +/- 14 345 +/- 18 397 +/- 27 330 +/- 14 0.070355 0.0101782 -0.061654 0.010118 0.1057453 RBM17 RNA binding motif protein 17 269 +/- 33 382 +/- 35 305 +/- 29 360 +/- 17 0.878038 0.0413517 0.146628 0.1058318 0.4964689 RBM19 RNA binding motif protein 19 727 +/- 55 514 +/- 46 537 +/- 38 381 +/- 60 -0.002644 -0.014212 -0.062673 0.07568 0.1984864 RBM25 RNA binding motif protein 25 1188 +/- 33 1453 +/- 33 1540 +/- 42 1830 +/- 101 3.38E-05 0.0002076 0.041769 0.0052 0.2262857 RBM3 RNA binding motif (RNP1, RRM) protein 3 669 +/- 111 1358 +/- 95 805 +/- 127 1331 +/- 55 0.377647 0.0008674 0.007144 0.0213976 0.0454136 RBM5 RNA binding motif protein 5 1495 +/- 88 1813 +/- 55 1547 +/- 80 1848 +/- 62 0.706355 0.0147948 0.016372 0.0280061 0.0253899 RBM8A RNA binding motif protein 8A 1042 +/- 66 948 +/- 45 1249 +/- 91 986 +/- 63 0.097058 -0.270772 -0.042824 0.2082881 0.0329119 RBM9 RNA binding motif protein 9 5783 +/- 65 4948 +/- 171 5058 +/- 152 4836 +/- 215 -0.057821 -0.003139 -0.424617 0.0028475 0.6246533 RBMS1 RNA binding motif, single stranded interacting protein 1 195 +/- 15 138 +/- 12 149 +/- 8 122 +/- 10 -0.023495 -0.016158 -0.059221 0.0416093 0.2834169 RCHY1 ring finger and CHY zinc finger domain containing 1 986 +/- 103 1349 +/- 54 982 +/- 90 1245 +/- 17 -0.368051 0.0152129 0.031787 0.05468 0.2021653 RCOR3 REST corepressor 3 986 +/- 96 990 +/- 27 1072 +/- 16 989 +/- 23 0.375004 0.9724902 -0.020621 0.7145102 0.0091227 RECK reversion-inducing-cysteine-rich protein with kazal motifs 328 +/- 29 364 +/- 13 406 +/- 21 327 +/- 12 0.334784 0.3037052 -0.011968 0.3841479 0.0449901 RECQL5 RecQ protein-like 5 88 +/- 21 145 +/- 16 75 +/- 7 129 +/- 9 -0.380111 0.0593871 0.001053 0.0101489 0.039322 REEP1 receptor accessory protein 1 3525 +/- 94 2995 +/- 201 2782 +/- 183 2618 +/- 100 -0.00511 -0.048147 -0.455031 0.0133104 0.8612964 RENBP renin binding protein 106 +/- 14 148 +/- 12 134 +/- 15 152 +/- 8 0.018497 0.053118 0.33391 0.1560433 0.2784616 REPS2 RALBP1 associated Eps domain containing 2 1087 +/- 40 1316 +/- 59 1227 +/- 99 1360 +/- 65 0.112952 0.0106745 0.295187 0.0300184 0.3975312 REV3L REV3-like, catalytic subunit of DNA polymerase zeta (yeast) 1626 +/- 79 1510 +/- 68 1694 +/- 74 1485 +/- 50 0.868533 -0.293951 -0.046104 0.2878013 0.0987565 REXO1 REX1, RNA exonuclease 1 homolog (S. cerevisiae) 655 +/- 28 517 +/- 30 915 +/- 58 744 +/- 67 0.000389 -0.007209 -0.08753 0.0019855 0.2431326 RFT1 RFT1 homolog (S. cerevisiae) 144 +/- 6 123 +/- 7 159 +/- 9 123 +/- 5 0.458934 -0.053412 -0.00949 0.1162332 0.0183997 RFXANK regulatory factor X-associated ankyrin-containing protein 382 +/- 19 443 +/- 19 312 +/- 22 350 +/- 23 -0.000451 0.0455876 0.256114 0.0324922 0.0503628 RGS11 regulator of G-protein signalling 11 262 +/- 54 525 +/- 62 397 +/- 50 606 +/- 54 0.016368 0.0094108 0.019498 0.0761906 0.0269708 RGS12 regulator of G-protein signalling 12 350 +/- 21 452 +/- 21 478 +/- 32 437 +/- 24 0.174224 0.0060087 -0.324886 0.0331131 0.4451116 RHEB Ras homolog enriched in brain 7133 +/- 176 6424 +/- 174 6526 +/- 211 6223 +/- 272 -0.097901 -0.016741 -0.40473 0.0010941 0.2249409 RHOBTB2 Rho-related BTB domain containing 2 686 +/- 32 533 +/- 29 663 +/- 45 502 +/- 41 -0.513856 -0.005245 -0.028314 0.0079489 0.0887015

S1- alphabetical list of aging-related genes.xls Page 26 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG RHOU ras homolog gene family, member U 2390 +/- 265 3326 +/- 556 1311 +/- 72 1705 +/- 52 -0.001259 0.1711621 0.00185 0.0287121 0.0028013 RICTOR rapamycin-insensitive companion of mTOR 2415 +/- 271 3115 +/- 219 2053 +/- 54 2477 +/- 109 -0.018334 0.073289 0.013266 0.0617395 0.0030748 RIF1 RAP1 interacting factor homolog (yeast) 637 +/- 107 956 +/- 124 515 +/- 28 671 +/- 28 -0.040163 0.080335 0.003329 0.0156319 0.0176562 RINT1 RAD50 interactor 1 379 +/- 15 428 +/- 6 425 +/- 14 401 +/- 17 0.39507 0.0175241 -0.303253 0.0913129 0.0759984 RIPK2 receptor-interacting serine-threonine kinase 2 475 +/- 23 551 +/- 22 420 +/- 19 435 +/- 19 -0.000169 0.040023 0.590228 0.2592377 0.9694159 RIT2 Ras-like without CAAX 2 609 +/- 31 437 +/- 55 775 +/- 62 568 +/- 48 0.002842 -0.025575 -0.027876 0.0778629 0.0628945 RNASE4 ribonuclease, RNase A family, 4 204 +/- 17 250 +/- 22 133 +/- 12 240 +/- 28 -0.003533 0.1324862 0.015739 0.2850226 0.0024759 RNASE6 ribonuclease, RNase A family, k6 397 +/- 35 654 +/- 73 255 +/- 16 436 +/- 47 -0.003229 0.0149096 0.014506 0.0062424 0.0746811 RNASEH1 ribonuclease H1 129 +/- 15 178 +/- 14 148 +/- 7 241 +/- 18 0.013749 0.0416065 0.004633 0.229431 0.0325456 RNASEH2Aribonuclease H2, large subunit 227 +/- 15 280 +/- 16 209 +/- 14 224 +/- 16 -0.051719 0.0374968 0.500722 0.1277155 0.7681737 RNF10 ring finger protein 10 3235 +/- 192 2478 +/- 121 3155 +/- 83 2677 +/- 130 0.583397 -0.009518 -0.01745 0.0827088 0.4093223 RNF130 ring finger protein 130 2915 +/- 322 3688 +/- 372 2157 +/- 119 2608 +/- 105 -0.00724 0.1481042 0.019517 0.0619848 0.0806561 RNF141 ring finger protein 141 443 +/- 75 613 +/- 119 430 +/- 48 621 +/- 45 -0.739558 0.2618356 0.017611 0.1060353 0.1070647 RNF149 ring finger protein 149 862 +/- 59 752 +/- 33 938 +/- 25 772 +/- 46 0.24407 -0.140754 -0.018644 0.2632535 0.0609607 RNF165 ring finger protein 165 582 +/- 25 444 +/- 19 525 +/- 34 419 +/- 29 -0.207282 -0.00155 -0.042494 0.0434461 0.1918806 RNF180 ring finger protein 180 252 +/- 11 196 +/- 5 237 +/- 14 213 +/- 19 0.986678 -0.002121 -0.336434 0.0101429 0.2583389 RNF187 ring finger protein 187 4466 +/- 174 4113 +/- 168 4156 +/- 120 3630 +/- 66 -0.038965 -0.175609 -0.005496 0.0383765 0.0309644 RNF40 ring finger protein 40 697 +/- 31 616 +/- 10 876 +/- 27 750 +/- 31 0.000102 -0.043587 -0.014865 0.3401724 0.1080425 RNGTT RNA guanylyltransferase and 5'-phosphatase 1248 +/- 42 1197 +/- 32 1228 +/- 26 1129 +/- 20 -0.223406 -0.358666 -0.01555 0.2173333 0.0250786 RNPS1 RNA binding protein S1, serine-rich domain 2748 +/- 78 3124 +/- 133 3034 +/- 149 3248 +/- 132 0.026594 0.0408598 0.310943 0.2161541 0.4479756 ROBO2 roundabout, axon guidance receptor, homolog 2 (Drosophila) 1844 +/- 48 1630 +/- 69 1649 +/- 73 1537 +/- 82 -0.030465 -0.032174 -0.337925 0.001026 0.6461609 RP11-217Himplantation-associated protein 540 +/- 57 800 +/- 92 433 +/- 35 574 +/- 40 -0.025838 0.0414708 0.027973 0.004277 0.1483887 RP13-297EDNA segment on chromosome X and Y (unique) 155 expressed sequence, isoform 1 610 +/- 39 823 +/- 49 675 +/- 31 664 +/- 44 -0.4263 0.0073577 -0.836699 0.0389275 0.9081344 RP2 retinitis pigmentosa 2 (X-linked recessive) 105 +/- 12 148 +/- 15 124 +/- 13 142 +/- 17 0.609397 0.0470855 0.433553 0.0756167 0.9130397 RPESP RPE-spondin 216 +/- 36 219 +/- 54 370 +/- 37 509 +/- 43 0.001557 0.9565057 0.036551 0.7215285 0.0174944 RPIB9 Rap2-binding protein 9 1388 +/- 111 1270 +/- 94 1665 +/- 42 1512 +/- 53 0.005251 -0.437014 -0.054718 0.4532659 0.3512872 RPL13 ribosomal protein L13 3331 +/- 242 4584 +/- 286 2546 +/- 122 3520 +/- 104 -9.29E-05 0.0076985 0.000189 0.0087231 0.0059545 RPL15 Ribosomal protein L15 201 +/- 25 146 +/- 14 188 +/- 13 126 +/- 10 -0.236996 -0.091943 -0.005182 0.1365207 0.0084855 RPL23AP1 ribosomal protein L23a pseudogene 13 491 +/- 25 617 +/- 43 598 +/- 72 594 +/- 30 0.193147 0.0359498 -0.961361 0.0245244 0.90389 RPL24 ribosomal protein L24 6992 +/- 211 7638 +/- 185 6738 +/- 313 6697 +/- 239 -0.021558 0.0444177 -0.919546 0.1335962 0.5753282 RPL30 ribosomal protein L30 15248 +/- 656 16050 +/- 750 15243 +/- 600 17322 +/- 644 0.248868 0.4397322 0.04333 0.6162516 0.0238786 RPL31 ribosomal protein L31 232 +/- 31 172 +/- 17 275 +/- 9 198 +/- 25 0.048221 -0.128385 -0.032936 0.3364343 0.2694518 RPL36AL ribosomal protein L36a-like 2981 +/- 70 3410 +/- 137 2856 +/- 113 3117 +/- 174 -0.074253 0.0256528 0.246812 0.1261708 0.0732023 RPL37 ribosomal protein L37 1311 +/- 97 1831 +/- 155 1601 +/- 107 1229 +/- 57 -0.522377 0.0208005 -0.016961 0.0313841 0.0721045 RPL6 ribosomal protein L6 10615 +/- 183 9845 +/- 191 10876 +/- 273 9843 +/- 437 0.385019 -0.015594 -0.0857 0.0602139 0.3123224 RPL9 ribosomal protein L9 12030 +/- 543 14358 +/- 285 10191 +/- 563 9811 +/- 1825 -0.000122 0.0062042 -0.850374 0.0454903 0.908068 RPP30 ribonuclease P/MRP 30kDa subunit 391 +/- 15 345 +/- 14 392 +/- 26 360 +/- 9 0.881613 -0.044504 -0.284057 0.014672 0.4228909 RPP40 ribonuclease P 40kDa subunit 814 +/- 28 680 +/- 26 723 +/- 28 663 +/- 58 -0.147894 -0.006009 -0.389147 0.0010622 0.3528995 RPRC1 arginine/proline rich coiled-coil 1 6598 +/- 221 6480 +/- 243 6871 +/- 111 7286 +/- 69 0.036021 -0.725681 0.013169 0.5078475 0.1042715 RPS12 ribosomal protein S12 4329 +/- 211 3150 +/- 239 3422 +/- 186 2508 +/- 191 -0.000234 -0.004219 -0.007636 0.0932139 0.1503967 RPS15A ribosomal protein S15a 14626 +/- 477 16659 +/- 556 11527 +/- 341 13684 +/- 211 -3.97E-06 0.019978 0.000642 0.0187529 0.0088016 RPS24 ribosomal protein S24 19440 +/- 754 21550 +/- 931 17416 +/- 712 21538 +/- 983 -0.088979 0.1101938 0.010136 0.358614 0.0975053 RPS25 ribosomal protein S25 403 +/- 49 270 +/- 30 272 +/- 35 279 +/- 26 -0.066277 -0.049243 0.872731 0.063496 0.9284774 RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 1507 +/- 66 2010 +/- 89 1589 +/- 57 1912 +/- 83 -0.830514 0.0013009 0.013807 0.0432698 0.1213117

S1- alphabetical list of aging-related genes.xls Page 27 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG RRAGC Ras-related GTP binding C 2912 +/- 161 3207 +/- 157 2579 +/- 91 2330 +/- 56 -0.000557 0.2207537 -0.047748 0.4301719 0.0631591 RRBP1 ribosome binding protein 1 homolog 180kDa (dog) 119 +/- 18 186 +/- 24 125 +/- 7 169 +/- 11 -0.733988 0.0494896 0.010814 0.0186224 0.014766 RSBN1 round spermatid basic protein 1 1261 +/- 90 1171 +/- 26 1341 +/- 54 1091 +/- 43 -0.957078 -0.373711 -0.005686 0.6094607 0.0586524 RTCD1 RNA terminal phosphate cyclase domain 1 145 +/- 9 194 +/- 12 137 +/- 5 174 +/- 10 -0.104237 0.0082334 0.01704 0.0120018 0.0005568 RTF1 Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) 1080 +/- 61 889 +/- 52 1157 +/- 39 1177 +/- 28 0.006301 -0.039212 0.687224 0.0166795 0.9882032 RTN3 reticulon 3 21006 +/- 1230 13544 +/- 1159 20556 +/- 206 13859 +/- 1090 -0.830862 -0.001314 -0.002946 0.0470105 0.0448787 RUFY3 RUN and FYVE domain containing 3 69 +/- 10 111 +/- 9 114 +/- 13 156 +/- 17 0.001984 0.0099158 0.096064 0.0138411 0.0161513 RUNX1T1 runt-related transcription factor 1; translocated to, 1 (cyclin D-related) 2489 +/- 125 1740 +/- 89 2274 +/- 171 1707 +/- 184 -0.409014 -0.000847 -0.051017 0.016456 0.4956507 RYBP RING1 and YY1 binding protein 922 +/- 102 1454 +/- 142 927 +/- 96 1306 +/- 164 -0.27578 0.0136345 0.088025 0.0688902 0.3124103 RYR1 ryanodine receptor 1 (skeletal) 1032 +/- 27 722 +/- 33 993 +/- 101 590 +/- 54 -0.212346 -3.08E-05 -0.008597 0.000353 0.0252594 S100A6 S100 calcium binding protein A6 (calcyclin) 923 +/- 64 1156 +/- 72 682 +/- 81 1085 +/- 58 -0.00207 0.0359624 0.003149 0.2202466 0.1084609 SAMD1 sterile alpha motif domain containing 1 363 +/- 22 312 +/- 35 561 +/- 23 439 +/- 31 2.83E-07 -0.249772 -0.013804 0.2150173 0.0153949 SAMD13 sterile alpha motif domain containing 13 234 +/- 23 238 +/- 21 261 +/- 20 314 +/- 8 0.027004 0.9033007 0.044956 0.9222472 0.2310658 SAMD4A sterile alpha motif domain containing 4A 1424 +/- 134 2109 +/- 170 1803 +/- 166 1808 +/- 59 0.63583 0.0108006 0.978024 0.0244032 0.9829683 SAP130 Sin3A-associated protein, 130kDa 1623 +/- 42 1504 +/- 24 1469 +/- 39 1472 +/- 40 -0.005698 -0.03916 0.964552 0.0232187 0.486987 SAP18 Sin3A-associated protein, 18kDa 2878 +/- 140 4280 +/- 172 2600 +/- 139 3721 +/- 168 -0.020479 0.0001016 0.000789 9.322E-05 0.0050428 SAPS3 SAPS domain family, member 3 485 +/- 34 610 +/- 18 601 +/- 21 616 +/- 32 0.056729 0.0133135 0.717097 0.0080744 0.9801772 SBK1 SH3-binding domain kinase 1 898 +/- 90 609 +/- 68 744 +/- 48 642 +/- 28 -0.583487 -0.029437 -0.101301 0.0578548 0.5750621 SC4MOL sterol-C4-methyl oxidase-like 5593 +/- 587 6927 +/- 418 5526 +/- 348 7175 +/- 378 0.869536 0.0972138 0.011246 0.2418129 0.0870044 SCD5 stearoyl-CoA desaturase 5 15204 +/- 701 15897 +/- 556 13728 +/- 231 15363 +/- 292 -0.060969 0.4577856 0.002264 0.3129803 0.0462543 SCFD1 sec1 family domain containing 1 83 +/- 2 121 +/- 10 105 +/- 6 109 +/- 8 0.715434 0.0094376 0.733605 0.0408128 0.5680569 SCGB3A1 secretoglobin, family 3A, member 1 110 +/- 8 98 +/- 11 132 +/- 9 101 +/- 7 0.278805 -0.404157 -0.023415 0.9165568 0.0928432 SCNM1 sodium channel modifier 1 257 +/- 18 318 +/- 18 261 +/- 8 288 +/- 10 -0.531044 0.0364379 0.059946 0.0305578 0.2725919 SCRN1 secernin 1 9838 +/- 238 10891 +/- 605 8473 +/- 141 10001 +/- 220 -0.003911 0.152799 0.000635 0.8102889 0.0171262 SCYE1 small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) 1313 +/- 43 1283 +/- 31 1364 +/- 27 1259 +/- 16 0.524237 -0.585045 -0.009865 0.670895 0.0321536 SDCCAG1 serologically defined colon cancer antigen 1 388 +/- 20 315 +/- 15 516 +/- 63 441 +/- 28 0.005473 -0.017003 -0.314805 0.0037034 0.1962015 SDCCAG8 serologically defined colon cancer antigen 8 262 +/- 8 225 +/- 12 276 +/- 23 239 +/- 4 0.48909 -0.027839 -0.167449 0.1029925 0.362649 SDHC succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa 1763 +/- 91 1634 +/- 45 1610 +/- 52 1461 +/- 35 -0.000608 -0.242924 -0.043667 0.2746808 0.025762 SDK2 sidekick homolog 2 (chicken) 931 +/- 129 836 +/- 94 667 +/- 46 527 +/- 27 -0.009243 -0.563399 -0.030214 0.5199325 0.0915624 SDS serine dehydratase 467 +/- 22 597 +/- 50 542 +/- 35 590 +/- 52 0.166932 0.0486479 0.469207 0.3174204 0.680392 SEC14L1 SEC14-like 1 (S. cerevisiae) 4771 +/- 153 4085 +/- 221 5018 +/- 284 3990 +/- 299 0.420052 -0.031449 -0.034708 0.4597004 0.2615953 SEC22A SEC22 vesicle trafficking protein homolog A (S. cerevisiae) 388 +/- 11 342 +/- 16 440 +/- 19 453 +/- 44 0.00208 -0.03712 0.804564 0.1895016 0.1610125 SEC22C SEC22 vesicle trafficking protein homolog C (S. cerevisiae) 250 +/- 23 183 +/- 15 231 +/- 16 204 +/- 13 0.902933 -0.03838 -0.216114 0.0244856 0.1285385 SEC23B Sec23 homolog B (S. cerevisiae) 1038 +/- 61 1069 +/- 58 923 +/- 23 1057 +/- 33 -0.045226 0.7178976 0.0106 0.7702871 0.0156109 SEC23IP SEC23 interacting protein 945 +/- 34 1092 +/- 23 868 +/- 39 1118 +/- 42 -0.482944 0.0065327 0.001979 0.017476 0.0363604 SEC61A1 Sec61 alpha 1 subunit (S. cerevisiae) 320 +/- 43 269 +/- 28 351 +/- 21 276 +/- 12 0.651065 -0.340419 -0.014546 0.3899871 0.0086829 SEC61G Sec61 gamma subunit 2171 +/- 111 2251 +/- 109 1907 +/- 76 2224 +/- 48 -0.105523 0.6173269 0.007253 0.7257383 0.0489315 SEH1L SEH1-like (S. cerevisiae) 297 +/- 24 216 +/- 13 286 +/- 18 233 +/- 18 0.98717 -0.01853 -0.067982 0.0875544 0.0323097 SEL1L sel-1 suppressor of lin-12-like (C. elegans) 350 +/- 53 493 +/- 66 413 +/- 38 592 +/- 39 0.343813 0.1217242 0.010126 0.1524493 0.1654514 SELPLG selectin P ligand 660 +/- 60 886 +/- 79 469 +/- 46 573 +/- 50 -0.001155 0.047568 0.159001 0.0551214 0.3252283 SELS selenoprotein S 2984 +/- 127 2979 +/- 209 2303 +/- 53 2615 +/- 85 -0.002081 -0.98617 0.017136 0.7899292 0.0790283 SEMA6B sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B 2141 +/- 281 1472 +/- 204 2572 +/- 104 2191 +/- 53 0.002351 -0.085956 -0.013093 0.1058834 0.195481 SEPT11 Septin 11 2461 +/- 262 1813 +/- 192 2281 +/- 37 1816 +/- 76 -0.517864 -0.076742 -0.00158 0.1117263 0.0423811 SEPT3 septin 3 7220 +/- 468 5490 +/- 263 8056 +/- 320 7357 +/- 258 0.000891 -0.012451 -0.1241 0.0213601 0.4560522

S1- alphabetical list of aging-related genes.xls Page 28 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG SEPT8 septin 8 2774 +/- 489 5315 +/- 965 1763 +/- 94 3266 +/- 249 -0.02563 0.0491891 0.002205 0.0143031 0.0038959 SERPINA1 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 442 +/- 80 836 +/- 138 331 +/- 29 562 +/- 91 -0.057256 0.0392633 0.062067 0.0050755 0.0643242 SERPINB6 serpin peptidase inhibitor, clade B (ovalbumin), member 6 139 +/- 16 197 +/- 19 232 +/- 20 249 +/- 10 0.003543 0.0405549 0.469389 0.2040456 0.7728342 SERPINB8 serpin peptidase inhibitor, clade B (ovalbumin), member 8 103 +/- 14 142 +/- 10 101 +/- 7 125 +/- 8 -0.229819 0.0451369 0.047732 0.2104028 0.5678892 SERPIND1serpin peptidase inhibitor, clade D (heparin cofactor), member 1 237 +/- 137 92 +/- 52 51 +/- 5 74 +/- 6 -0.347633 -0.356912 0.02389 0.1291434 0.0302886 SERPINF1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), 508 +/- 96 868 +/- 124 280 +/- 49 579 +/- 121 -0.001876 0.0462446 0.067889 0.2048228 0.1776994 SET SET translocation (myeloid leukemia-associated) 698 +/- 22 583 +/- 14 1015 +/- 52 866 +/- 35 2.46E-07 -0.002193 -0.04242 0.020561 0.0522707 SETBP1 SET binding protein 1 177 +/- 22 138 +/- 17 245 +/- 24 131 +/- 20 0.139943 -0.191834 -0.005449 0.4480021 0.1355688 SETD6 SET domain containing 6 357 +/- 14 262 +/- 22 362 +/- 8 285 +/- 23 0.351473 -0.006155 -0.024761 0.0713392 0.1575501 SETX senataxin 1154 +/- 76 1267 +/- 18 1110 +/- 19 1244 +/- 44 -0.478762 0.2036891 0.033306 0.2141339 6.061E-05 SEZ6L2 Seizure related 6 homolog (mouse)-like 2 1565 +/- 18 1244 +/- 115 2230 +/- 72 2186 +/- 114 0.000161 -0.038027 -0.756044 0.0439181 0.9303992 SF1 splicing factor 1 140 +/- 11 201 +/- 16 207 +/- 11 256 +/- 26 0.000508 0.01371 0.143512 0.0146115 0.103229 SF3A3 Splicing factor 3a, subunit 3, 60kDa 3426 +/- 274 2814 +/- 133 2964 +/- 63 2412 +/- 70 -0.010701 -0.082988 -0.000278 0.0193853 0.0052495 SFRP2 secreted frizzled-related protein 2 277 +/- 172 120 +/- 40 22 +/- 6 268 +/- 36 -0.993815 -0.415931 0.001974 0.2592274 0.0514684 SFRS11 splicing factor, arginine/serine-rich 11 4017 +/- 267 5453 +/- 187 4660 +/- 175 5391 +/- 406 0.162392 0.0017133 0.153883 0.0002397 0.0174607 SFRS14 splicing factor, arginine/serine-rich 14 2501 +/- 104 2096 +/- 137 2785 +/- 51 2631 +/- 85 0.00293 -0.04162 -0.167002 0.0062501 0.0353494 SFRS7 splicing factor, arginine/serine-rich 7, 35kDa 2011 +/- 79 1722 +/- 78 1994 +/- 57 1707 +/- 55 -0.903556 -0.026627 -0.005697 0.0569491 0.0114376 SFT2D1 SFT2 domain containing 1 2004 +/- 101 2392 +/- 168 1748 +/- 92 2190 +/- 64 -0.063607 0.0826378 0.003836 0.0186036 0.0524282 SFXN1 sideroflexin 1 947 +/- 84 649 +/- 46 800 +/- 33 630 +/- 29 -0.014836 -0.014754 -0.004025 0.0228448 0.0559987 SFXN3 sideroflexin 3 918 +/- 58 841 +/- 34 1028 +/- 50 787 +/- 44 0.596632 -0.290513 -0.005491 0.5475952 0.0698529 SFXN5 Sideroflexin 5 157 +/- 8 208 +/- 14 190 +/- 13 226 +/- 9 0.010934 0.0109709 0.05192 0.0002757 0.0306315 SGCE sarcoglycan, epsilon 754 +/- 60 1219 +/- 49 868 +/- 51 1204 +/- 28 0.231804 0.0001575 0.000483 0.0032894 0.0064522 SGEF Src homology 3 domain-containing guanine nucleotide exchange factor 828 +/- 77 772 +/- 31 904 +/- 53 676 +/- 59 -0.971235 -0.51893 -0.01897 0.8045084 0.0061152 SGK serum/glucocorticoid regulated kinase 1969 +/- 150 2626 +/- 225 1220 +/- 179 1632 +/- 102 -0.000539 0.0390527 0.08161 0.0471041 0.2118836 SGPL1 sphingosine-1-phosphate lyase 1 613 +/- 28 522 +/- 26 640 +/- 24 524 +/- 12 0.561927 -0.040206 -0.002866 0.2319508 0.0578856 SGSH N-sulfoglucosamine sulfohydrolase (sulfamidase) 274 +/- 62 276 +/- 24 224 +/- 8 190 +/- 9 -0.056384 0.9756562 -0.023478 0.8671328 0.1881173 SH2B SH2-B homolog 1071 +/- 20 1347 +/- 59 1612 +/- 117 1439 +/- 90 0.013408 0.0041889 -0.272413 0.046181 0.3927911 SH3BGRL SH3 domain binding glutamic acid-rich protein like 1959 +/- 216 2188 +/- 169 2416 +/- 113 2842 +/- 144 0.014296 0.4222461 0.048322 0.3057342 0.2728617 SH3GL1 SH3-domain GRB2-like 1 517 +/- 28 419 +/- 30 649 +/- 35 550 +/- 32 0.000487 -0.037857 -0.069011 0.062626 0.4344739 SH3MD4 SH3 multiple domains 4 1732 +/- 142 1583 +/- 171 1400 +/- 71 1205 +/- 43 -0.003383 -0.518297 -0.047787 0.9282667 0.2591898 SH3PX3 SH3 and PX domain containing 3 243 +/- 24 325 +/- 26 209 +/- 22 227 +/- 14 -0.015925 0.0443613 0.515619 0.0464582 0.7937885 SHANK2 SH3 and multiple ankyrin repeat domains 2 3124 +/- 137 2183 +/- 63 2942 +/- 130 2120 +/- 154 -0.403233 -0.000418 -0.003277 0.0009546 0.0749451 SHMT2 serine hydroxymethyltransferase 2 (mitochondrial) 193 +/- 15 139 +/- 8 177 +/- 12 147 +/- 8 -0.723763 -0.014907 -0.071431 0.0316628 0.1098978 SIN3B SIN3 homolog B, transcription regulator (yeast) 1456 +/- 63 1539 +/- 57 1516 +/- 56 1759 +/- 48 0.062398 0.3464777 0.009402 0.9941848 0.022693 SIRT6 sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) 255 +/- 17 215 +/- 16 279 +/- 15 203 +/- 7 0.692921 -0.116656 -0.002473 0.0531824 0.0015775 SKIV2L superkiller viralicidic activity 2-like (S. cerevisiae) 624 +/- 40 474 +/- 21 631 +/- 36 490 +/- 27 0.53802 -0.011364 -0.012191 0.0067653 0.0223014 SLA/LP soluble liver antigen/liver pancreas antigen 90 +/- 8 130 +/- 15 95 +/- 11 94 +/- 4 -0.230051 0.044906 -0.958316 0.0342527 0.9074774 SLC11A2 solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 475 +/- 88 1020 +/- 94 461 +/- 69 952 +/- 81 -0.338113 0.0017448 0.001506 0.009127 0.01046 SLC12A5 solute carrier family 12, (potassium-chloride transporter) member 5 9260 +/- 395 7923 +/- 381 8510 +/- 287 7677 +/- 214 -0.162509 -0.035166 -0.045788 0.0154776 0.2021898 SLC12A9 solute carrier family 12 (potassium/chloride transporters), member 9 1093 +/- 101 1413 +/- 80 1023 +/- 47 1257 +/- 43 -0.069404 0.0331331 0.005051 0.1444962 0.1520423 SLC13A4 solute carrier family 13 (sodium/sulfate symporters), member 4 758 +/- 418 154 +/- 92 35 +/- 8 120 +/- 24 -0.213465 -0.212461 0.019909 0.2274349 0.0059444 SLC14A1 Solute carrier family 14 (urea transporter), member 1 (Kidd blood group) 669 +/- 53 1000 +/- 92 657 +/- 68 883 +/- 90 -0.418944 0.0141667 0.079572 0.002607 0.1256663 SLC15A3 solute carrier family 15, member 3 255 +/- 27 313 +/- 19 234 +/- 7 271 +/- 8 -0.020326 0.1124988 0.005854 0.1031174 0.0062236 SLC15A4 Solute carrier family 15, member 4 630 +/- 46 885 +/- 55 671 +/- 24 756 +/- 14 -0.434673 0.0053372 0.014982 0.0129992 0.036015

S1- alphabetical list of aging-related genes.xls Page 29 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG SLC16A14 solute carrier family 16 (monocarboxylic acid transporters), member 14 1075 +/- 41 1004 +/- 75 1195 +/- 34 1038 +/- 52 0.036333 -0.435972 -0.03911 0.4885692 0.0290485 SLC1A4 solute carrier family 1 (glutamate/neutral amino acid transporter), member 4 3466 +/- 213 3097 +/- 143 3093 +/- 119 2554 +/- 111 -0.007805 -0.185181 -0.009141 0.0994061 0.0194672 SLC22A18 solute carrier family 22 (organic cation transporter), member 18 269 +/- 23 400 +/- 39 191 +/- 11 317 +/- 21 -0.002158 0.0198389 0.00162 0.1136965 0.0449552 SLC24A5 Solute carrier family 24, member 5 1183 +/- 81 1115 +/- 57 1455 +/- 56 1211 +/- 48 0.028959 -0.514493 -0.008949 0.3598755 0.0032771 SLC25A16 solute carrier family 25 (mitochondrial carrier; Graves disease autoantigen), member 16 472 +/- 27 547 +/- 23 566 +/- 28 661 +/- 17 0.001135 0.0629683 0.021427 0.4766455 0.0789001 SLC25A18 solute carrier family 25 (mitochondrial carrier), member 18 2127 +/- 291 3200 +/- 170 2193 +/- 184 3259 +/- 127 0.481108 0.0129141 0.001186 0.0504416 0.0149975 SLC25A25 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 1121 +/- 61 974 +/- 52 1300 +/- 67 1101 +/- 43 0.028599 -0.096748 -0.036721 0.0306786 0.1145057 SLC26A2 solute carrier family 26 (sulfate transporter), member 2 87 +/- 10 217 +/- 49 135 +/- 50 223 +/- 61 0.418715 0.0457219 0.294341 0.1288492 0.2520888 SLC2A5 solute carrier family 2 (facilitated glucose/fructose transporter), member 5 534 +/- 45 707 +/- 82 446 +/- 26 613 +/- 49 -0.166705 0.1022042 0.023848 0.1259176 0.2899389 SLC2A6 solute carrier family 2 (facilitated glucose transporter), member 6 542 +/- 34 512 +/- 27 621 +/- 36 490 +/- 11 0.406867 -0.497871 -0.013618 0.5270778 0.1016912 SLC30A5 solute carrier family 30 (zinc transporter), member 5 457 +/- 12 540 +/- 14 505 +/- 21 552 +/- 21 0.086592 0.0011692 0.145466 0.0041833 0.1944157 SLC31A1 solute carrier family 31 (copper transporters), member 1 325 +/- 19 228 +/- 24 334 +/- 28 277 +/- 30 0.229054 -0.011782 -0.198781 0.03351 0.5673277 SLC35A2 solute carrier family 35 (UDP-galactose transporter), member A2 628 +/- 18 538 +/- 17 657 +/- 30 593 +/- 23 0.095799 -0.004802 -0.127267 0.0158227 0.2921415 SLC35E3 solute carrier family 35, member E3 812 +/- 93 775 +/- 32 861 +/- 58 712 +/- 17 -0.905383 -0.724466 -0.049851 0.9435954 0.0795312 SLC37A4 solute carrier family 37 (glycerol-6-phosphate transporter), member 4 195 +/- 20 266 +/- 23 170 +/- 18 236 +/- 25 -0.092963 0.0446113 0.068065 0.1746472 0.2769666 SLC39A3 solute carrier family 39 (zinc transporter), member 3 529 +/- 35 429 +/- 22 453 +/- 33 436 +/- 14 -0.066127 -0.039489 -0.651066 0.0072498 0.5052375 SLC39A4 solute carrier family 39 (zinc transporter), member 4 534 +/- 35 698 +/- 42 516 +/- 28 599 +/- 44 -0.094072 0.0142727 0.152657 0.2165457 0.061296 SLC45A3 solute carrier family 45, member 3 194 +/- 24 304 +/- 46 108 +/- 28 201 +/- 22 -0.005552 0.0675492 0.027744 0.0448504 0.0220202 SLC4A7 solute carrier family 4, sodium bicarbonate cotransporter, member 7 1387 +/- 232 3599 +/- 647 1815 +/- 290 4114 +/- 614 0.096811 0.0171541 0.015698 0.028831 0.0115593 SLC5A11 solute carrier family 5 (sodium/glucose cotransporter), member 11 675 +/- 128 1122 +/- 169 389 +/- 31 607 +/- 40 -0.006695 0.063233 0.002558 0.0506597 0.0129141 SLC5A3 solute carrier family 5 (inositol transporters), member 3 289 +/- 39 240 +/- 38 429 +/- 33 342 +/- 17 0.000569 -0.391169 -0.052976 0.3881746 0.0789157 SLCO1C1 solute carrier organic anion transporter family, member 1C1 525 +/- 72 446 +/- 45 404 +/- 18 280 +/- 27 -0.008426 -0.380552 -0.006315 0.5831536 0.0395767 SLFNL1 schlafen-like 1 165 +/- 16 140 +/- 13 183 +/- 10 119 +/- 9 -0.983024 -0.23417 -0.001116 0.1409249 0.0660907 SMARCC1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, m 482 +/- 28 591 +/- 24 542 +/- 16 570 +/- 13 0.379305 0.0152509 0.203948 0.0043738 0.0863637 SMCHD1 structural maintenance of chromosomes flexible hinge domain containing 1 694 +/- 40 886 +/- 59 797 +/- 51 844 +/- 38 0.526667 0.0252493 0.477217 0.0001743 0.4448773 SMG7 Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) 162 +/- 20 141 +/- 13 158 +/- 9 189 +/- 8 0.291061 -0.403041 0.024009 0.4951647 0.2251241 SMS spermine synthase 1440 +/- 104 1361 +/- 60 1756 +/- 49 1533 +/- 42 0.001876 -0.531937 -0.00736 0.6064133 0.1179732 SMU1 Smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) 1021 +/- 44 845 +/- 49 1000 +/- 21 892 +/- 31 0.756623 -0.024101 -0.021558 0.0619541 0.1675365 SMYD2 SET and MYND domain containing 2 109 +/- 17 181 +/- 16 144 +/- 19 192 +/- 26 0.015144 0.0113553 0.180434 0.003035 0.0154565 SNAP25 Synaptosomal-associated protein, 25kDa 583 +/- 70 304 +/- 41 826 +/- 34 518 +/- 59 0.001182 -0.008466 -0.00321 0.0112881 0.0123304 SNAP91 synaptosomal-associated protein, 91kDa homolog (mouse) 10224 +/- 579 10352 +/- 809 10870 +/- 483 12419 +/- 359 0.016655 0.9003596 0.030731 0.7005781 0.2501644 SNAPC5 small nuclear RNA activating complex, polypeptide 5, 19kDa 221 +/- 25 289 +/- 15 232 +/- 9 285 +/- 45 0.995998 0.0457243 0.302887 0.260286 0.4904325 SNF1LK SNF1-like kinase 448 +/- 53 272 +/- 52 268 +/- 49 172 +/- 19 -0.002689 -0.038141 -0.114625 0.0501826 0.2906952 SNF8 SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) 2300 +/- 87 2382 +/- 140 1932 +/- 69 2281 +/- 69 -0.06192 0.6337008 0.005861 0.9171056 0.0610403 SNORA24 Small nucleolar RNA, H/ACA box 24 4609 +/- 197 5659 +/- 543 3508 +/- 255 4534 +/- 188 -0.00027 0.1169872 0.010709 0.2964168 0.0667392 SNRPG small nuclear ribonucleoprotein polypeptide G 658 +/- 21 808 +/- 50 678 +/- 26 740 +/- 42 -0.602182 0.0276006 0.250459 0.0004022 0.1002554 SNX1 Sorting nexin 1 172 +/- 19 261 +/- 27 175 +/- 13 234 +/- 16 -0.780355 0.0242684 0.020265 0.0134602 0.2426572 SNX17 sorting nexin 17 2369 +/- 40 2361 +/- 64 1963 +/- 54 2132 +/- 51 -1.88E-05 -0.920823 0.050425 0.208648 0.3840665 SNX3 sorting nexin 3 14461 +/- 818 11436 +/- 483 13459 +/- 458 11655 +/- 653 -0.295057 -0.012676 -0.055872 0.0689298 0.4183005 SNX9 sorting nexin 9 1081 +/- 34 1199 +/- 60 1155 +/- 37 1282 +/- 40 0.012901 0.1251068 0.045268 0.0200866 0.0494946 SOCS1 suppressor of cytokine signaling 1 193 +/- 12 114 +/- 15 214 +/- 14 159 +/- 18 0.001196 -0.002148 -0.038543 0.0006493 0.0014373 SOHLH2 spermatogenesis and oogenesis specific basic helix-loop-helix 2 101 +/- 10 163 +/- 15 88 +/- 6 159 +/- 11 -0.295007 0.0070528 0.001347 0.1139626 0.0336817 SORBS1 sorbin and SH3 domain containing 1 688 +/- 45 536 +/- 41 818 +/- 42 620 +/- 39 0.045933 -0.032044 -0.007103 0.0445536 0.0045809 SORBS2 Sorbin and SH3 domain containing 2 432 +/- 19 353 +/- 14 405 +/- 14 294 +/- 41 -0.075433 -0.007595 -0.049321 0.0784408 0.3642089

S1- alphabetical list of aging-related genes.xls Page 30 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG SOS2 Son of sevenless homolog 2 (Drosophila) 298 +/- 20 354 +/- 13 311 +/- 25 351 +/- 34 0.820968 0.0394209 0.369756 0.0328588 0.4459191 SOX1 SRY (sex determining region Y)-box 1 375 +/- 39 272 +/- 18 455 +/- 28 336 +/- 23 0.006275 -0.050161 -0.010004 0.1370089 0.0085711 SOX11 SRY (sex determining region Y)-box 11 664 +/- 52 459 +/- 46 303 +/- 32 221 +/- 13 -9.38E-06 -0.014598 -0.048754 0.0117623 0.0555967 SOX2 SRY (sex determining region Y)-box 2 4887 +/- 267 6273 +/- 249 4911 +/- 290 5382 +/- 228 -0.106097 0.0035426 0.233451 0.0098802 0.654743 SPA17 sperm autoantigenic protein 17 537 +/- 65 330 +/- 45 443 +/- 23 329 +/- 17 -0.506302 -0.027597 -0.003344 0.094749 0.0312364 SPAG9 sperm associated antigen 9 477 +/- 60 705 +/- 50 407 +/- 40 498 +/- 44 -0.007115 0.0161276 0.158938 0.0151246 0.0335196 SPCS1 signal peptidase complex subunit 1 homolog (S. cerevisiae) 6159 +/- 178 6206 +/- 222 5341 +/- 222 6203 +/- 226 -0.008114 0.8729474 0.023845 0.565696 0.2051347 SPG20 spastic paraplegia 20, spartin (Troyer syndrome) 1329 +/- 71 1486 +/- 143 1459 +/- 35 1302 +/- 45 -0.826379 0.3571879 -0.02541 0.0228355 0.1961751 SPG21 spastic paraplegia 21 (autosomal recessive, Mast syndrome) 2258 +/- 168 2325 +/- 93 1675 +/- 47 1917 +/- 25 -0.000469 0.7353056 0.002344 0.9724206 0.0643185 SPINK2 serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor) 187 +/- 45 123 +/- 5 124 +/- 3 177 +/- 17 -0.848383 -0.214183 0.037427 0.3169453 0.0206818 SPSB3 splA/ryanodine receptor domain and SOCS box containing 3 474 +/- 49 493 +/- 23 461 +/- 22 396 +/- 17 -0.137979 0.7266565 -0.049146 0.7436191 0.0334969 SPTBN1 spectrin, beta, non-erythrocytic 1 1270 +/- 153 835 +/- 88 1365 +/- 200 1056 +/- 161 0.020923 -0.038683 -0.260652 0.1486036 0.4901887 SPTLC1 serine palmitoyltransferase, long chain base subunit 1 2170 +/- 85 2440 +/- 104 1815 +/- 58 2111 +/- 70 -0.000449 0.0739814 0.010986 0.0123306 0.0526412 SPTY2D1 SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) 213 +/- 23 140 +/- 13 201 +/- 16 189 +/- 22 0.484866 -0.025219 -0.667917 0.0458095 0.2302019 SR-A1 serine arginine-rich pre-mRNA splicing factor SR-A1 440 +/- 30 527 +/- 58 660 +/- 33 789 +/- 43 3.71E-05 0.2204766 0.046113 0.8153769 0.1057452 SRBD1 S1 RNA binding domain 1 122 +/- 36 182 +/- 29 85 +/- 6 173 +/- 17 -0.630624 0.2292686 0.004933 0.1758022 0.0027783 SRCAP Snf2-related CBP activator protein 190 +/- 10 242 +/- 8 232 +/- 5 203 +/- 9 0.830548 0.0022256 -0.025474 0.0125831 0.0369587 SRD5A1 steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenas 264 +/- 147 539 +/- 62 255 +/- 133 609 +/- 56 0.588714 0.130382 0.04472 0.2397767 0.0728013 SREBF2 sterol regulatory element binding transcription factor 2 1173 +/- 80 1157 +/- 64 1650 +/- 13 1844 +/- 70 1.99E-05 -0.882991 0.049562 0.5901255 0.2589213 SRP19 signal recognition particle 19kDa 2698 +/- 166 3239 +/- 67 2572 +/- 101 3210 +/- 30 -0.287468 0.0210947 0.001033 0.1127686 0.0121634 SRrp35 serine-arginine repressor protein (35 kDa) 851 +/- 61 650 +/- 57 860 +/- 61 692 +/- 42 0.721963 -0.036873 -0.051147 0.0637382 0.1931891 SS18 synovial sarcoma translocation, chromosome 18 179 +/- 34 427 +/- 43 209 +/- 34 419 +/- 49 0.352157 0.0012461 0.00897 0.0029971 0.0621581 SS18L2 synovial sarcoma translocation gene on chromosome 18-like 2 2273 +/- 100 2573 +/- 55 2299 +/- 38 2484 +/- 84 -0.719714 0.0306707 0.093923 0.0663328 0.018152 SSB Sjogren syndrome antigen B (autoantigen La) 2114 +/- 118 1777 +/- 99 2359 +/- 70 1882 +/- 138 0.063217 -0.054698 -0.021628 0.0612614 0.1620291 SSBP2 Single-stranded DNA binding protein 2 148 +/- 42 473 +/- 63 131 +/- 36 339 +/- 32 -0.014338 0.0021597 0.00188 0.0049527 0.0027213 SSR2 signal sequence receptor, beta (translocon-associated protein beta) 2068 +/- 137 1585 +/- 78 1623 +/- 93 1483 +/- 68 -0.002111 -0.01556 -0.256528 0.0048081 0.1498578 SSR3 signal sequence receptor, gamma (translocon-associated protein gamma) 1416 +/- 40 1776 +/- 112 1570 +/- 46 1920 +/- 158 0.082192 0.022161 0.090364 0.1522552 0.6361821 SSTR2 somatostatin receptor 2 181 +/- 26 94 +/- 7 135 +/- 18 66 +/- 7 -0.029494 -0.018012 -0.010197 0.0054803 0.0554253 SSU72 SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae) 2015 +/- 93 2625 +/- 115 1716 +/- 93 2050 +/- 54 -0.001308 0.0022663 0.015168 0.1119429 0.0672956 SSX2IP synovial sarcoma, X breakpoint 2 interacting protein 256 +/- 17 190 +/- 21 218 +/- 7 166 +/- 12 -0.085889 -0.036497 -0.007626 0.0327467 0.0090246 ST3GAL6 ST3 beta-galactoside alpha-2,3-sialyltransferase 6 690 +/- 106 1161 +/- 162 768 +/- 42 966 +/- 63 -0.437144 0.0390735 0.033662 0.0120219 0.074534 ST6GALNAST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6 172 +/- 52 131 +/- 25 89 +/- 27 181 +/- 22 -0.762714 -0.494846 0.027556 0.7086499 0.1969843 ST6GALNAST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6 776 +/- 23 926 +/- 60 825 +/- 32 928 +/- 25 0.614432 0.0553481 0.031677 0.0060311 0.3455751 ST6GALNAST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6 422 +/- 12 305 +/- 23 413 +/- 44 385 +/- 11 0.219607 -0.002102 -0.562837 0.0090066 0.8653916 ST8SIA2 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 339 +/- 35 212 +/- 17 124 +/- 23 93 +/- 11 -6.27E-05 -0.013714 -0.261209 0.0369402 0.6086224 ST8SIA3 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3 2875 +/- 542 3178 +/- 335 2260 +/- 206 3420 +/- 132 -0.708791 0.6458159 0.001363 0.8601663 0.0345403 STARD3NLSTARD3 N-terminal like 1387 +/- 85 942 +/- 16 1203 +/- 86 834 +/- 52 -0.044391 -0.003026 -0.006158 0.0077875 0.0443081 STAT2 signal transducer and activator of transcription 2, 113kDa 863 +/- 108 1063 +/- 113 815 +/- 45 976 +/- 46 -0.59488 0.2297582 0.034695 0.1012264 0.0698112 STAT5B signal transducer and activator of transcription 5B 521 +/- 41 647 +/- 33 390 +/- 30 402 +/- 19 -0.000162 0.0378386 0.730509 0.0959601 0.5692423 STK11IP serine/threonine kinase 11 interacting protein 832 +/- 22 692 +/- 33 760 +/- 43 675 +/- 37 -0.140135 -0.007128 -0.167253 0.2201706 0.617637 STMN2 stathmin-like 2 15575 +/- 785 11635 +/- 678 13350 +/- 985 11673 +/- 910 -0.111545 -0.003626 -0.242694 0.0123956 0.5208959 STOM stomatin 2053 +/- 253 2990 +/- 261 1953 +/- 163 2755 +/- 171 -0.159255 0.0275157 0.008201 0.0632377 0.1575077 STRBP spermatid perinuclear RNA binding protein 926 +/- 84 754 +/- 29 785 +/- 30 663 +/- 42 -0.037528 -0.098304 -0.049348 0.2126192 0.2508432 STRC stereocilin 345 +/- 54 551 +/- 31 332 +/- 31 472 +/- 34 -0.132417 0.0108514 0.015354 0.0540734 0.0744316

S1- alphabetical list of aging-related genes.xls Page 31 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG STS steroid sulfatase (microsomal), arylsulfatase C, isozyme S 522 +/- 29 428 +/- 25 424 +/- 42 346 +/- 14 -0.021387 -0.033391 -0.124594 0.0267615 0.1852603 SUB1 SUB1 homolog (S. cerevisiae) 198 +/- 12 153 +/- 13 233 +/- 7 187 +/- 11 0.008317 -0.029381 -0.009334 0.0705728 0.2008498 SUGT1 SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) 266 +/- 18 181 +/- 12 342 +/- 23 283 +/- 32 0.000158 -0.003216 -0.181555 0.0325977 0.7391313 SUGT1L1 SGT1, suppressor of G2 allele of SKP1 like 1 (S. cerevisiae) 240 +/- 35 130 +/- 4 288 +/- 39 162 +/- 19 0.1329 -0.024887 -0.02192 0.0184495 0.0371058 SUHW3 suppressor of hairy wing homolog 3 (Drosophila) 164 +/- 8 195 +/- 7 232 +/- 8 239 +/- 11 0.000226 0.0123894 0.663982 0.0018506 0.4146132 SULT1A3 sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3 375 +/- 64 226 +/- 35 292 +/- 26 202 +/- 21 -0.066602 -0.078388 -0.02414 0.2439979 0.1542381 SUOX sulfite oxidase 266 +/- 15 222 +/- 19 301 +/- 8 220 +/- 19 0.242459 -0.100405 -0.008879 0.261112 0.0967416 SUPT4H1 suppressor of Ty 4 homolog 1 (S. cerevisiae) 854 +/- 45 1062 +/- 48 905 +/- 30 1088 +/- 32 0.110911 0.0099546 0.0024 0.0533066 0.1139936 SUPT7L suppressor of Ty 7 (S. cerevisiae)-like 547 +/- 33 403 +/- 23 594 +/- 42 454 +/- 21 0.073236 -0.005811 -0.01997 0.012705 0.0286548 SURB7 SRB7 suppressor of RNA polymerase B homolog (yeast) 1618 +/- 167 2473 +/- 241 1436 +/- 158 2200 +/- 169 -0.002882 0.0173654 0.00959 0.0264854 0.0236746 SURF1 surfeit 1 1906 +/- 52 2026 +/- 101 1667 +/- 86 1959 +/- 45 -0.001352 0.3240518 0.018135 0.7308047 0.0332671 SURF4 surfeit 4 844 +/- 29 730 +/- 19 751 +/- 33 661 +/- 21 -0.005552 -0.010483 -0.048623 0.1021851 0.242073 SYNGR4 synaptogyrin 4 345 +/- 36 312 +/- 23 289 +/- 21 221 +/- 22 -0.008731 -0.459799 -0.054898 0.6978628 0.2609005 SYNJ2BP Synaptojanin 2 binding protein 752 +/- 43 787 +/- 33 1031 +/- 19 944 +/- 14 6.14E-05 0.5393535 -0.005859 0.1846546 0.0128158 SYNPO2L synaptopodin 2-like 274 +/- 17 355 +/- 22 261 +/- 23 265 +/- 16 -0.049675 0.0168779 0.89537 0.025081 0.8519871 SYT11 synaptotagmin XI 2979 +/- 924 523 +/- 91 2747 +/- 804 738 +/- 96 -0.719216 -0.0449 -0.054405 0.0455452 0.0918564 SYT17 synaptotagmin XVII 1654 +/- 367 1225 +/- 137 1568 +/- 101 1227 +/- 51 -0.965794 -0.314314 -0.01883 0.2654489 0.0557006 SYTL2 synaptotagmin-like 2 300 +/- 78 429 +/- 112 148 +/- 20 94 +/- 10 -0.006273 0.368996 -0.048362 0.4373566 0.1563739 TAB3 TAK1-binding protein 3 1451 +/- 63 1273 +/- 30 1302 +/- 27 1272 +/- 19 -0.1265 -0.036765 -0.391402 0.0272875 0.3710045 TACC1 transforming, acidic coiled-coil containing protein 1 1582 +/- 114 2047 +/- 42 1759 +/- 107 2070 +/- 195 0.362427 0.0077848 0.207245 0.0065097 0.1016656 TADA1L transcriptional adaptor 1 (HFI1 homolog, yeast)-like 1627 +/- 54 1500 +/- 42 1705 +/- 66 1508 +/- 31 0.491764 -0.095764 -0.030645 0.385539 0.048167 TADA3L transcriptional adaptor 3 (NGG1 homolog, yeast)-like 1631 +/- 31 1444 +/- 60 1480 +/- 44 1513 +/- 52 -0.522145 -0.025412 0.647547 0.0317519 0.5628612 TAF1A TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa 204 +/- 13 258 +/- 14 251 +/- 18 253 +/- 11 0.110518 0.0173625 0.923978 0.0024907 0.9106105 TAF1B TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa 234 +/- 23 246 +/- 38 330 +/- 22 422 +/- 23 0.000316 0.7891915 0.019503 0.8921318 0.0425003 TAF3 TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa 296 +/- 8 367 +/- 26 310 +/- 6 301 +/- 21 -0.114659 0.03724 -0.710751 0.0028884 0.3174497 TAF5L TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kD 511 +/- 14 496 +/- 10 521 +/- 15 459 +/- 15 -0.489525 -0.402736 -0.015641 0.6288704 0.1895366 TALDO1 transaldolase 1 955 +/- 59 1652 +/- 264 1013 +/- 64 1727 +/- 292 0.631178 0.0454565 0.069641 0.0718661 0.0513873 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) 270 +/- 14 334 +/- 23 295 +/- 13 305 +/- 17 -0.861563 0.0462617 0.667673 0.4137277 0.6093939 TAS2R14 Taste receptor, type 2, member 14 101 +/- 20 203 +/- 19 104 +/- 21 230 +/- 27 0.395906 0.0044384 0.005974 0.0368933 0.0982432 TASP1 taspase, threonine aspartase, 1 270 +/- 19 225 +/- 18 323 +/- 12 254 +/- 14 0.006132 -0.113118 -0.005654 0.0680737 0.0494189 TATDN3 TatD DNase domain containing 3 116 +/- 9 169 +/- 11 138 +/- 16 192 +/- 10 0.063554 0.0041436 0.021647 0.0724146 0.0553409 TBC1D14 TBC1 domain family, member 14 2125 +/- 84 1815 +/- 92 1795 +/- 52 1639 +/- 43 -0.001141 -0.031696 -0.047245 0.2222672 0.2088684 TBC1D16 TBC1 domain family, member 16 132 +/- 15 84 +/- 12 134 +/- 13 147 +/- 12 0.129378 -0.030785 0.494538 0.016613 0.5945469 TBC1D17 TBC1 domain family, member 17 1262 +/- 103 1539 +/- 80 1047 +/- 42 1261 +/- 39 -0.007657 0.0615205 0.004927 0.0854102 0.0422091 TBC1D22BTBC1 domain family, member 22B 1294 +/- 39 1120 +/- 36 1174 +/- 53 1149 +/- 18 -0.33937 -0.008401 -0.662916 0.0040568 0.8721195 TBC1D2B TBC1 domain family, member 2B 261 +/- 20 351 +/- 28 327 +/- 28 322 +/- 34 0.379198 0.0283692 -0.924665 0.0893058 0.4755989 TBC1D9 TBC1 domain family, member 9 5875 +/- 79 5392 +/- 40 4902 +/- 118 5160 +/- 124 -0.000105 -0.000793 0.165632 0.0006887 0.6018428 TBCA tubulin-specific chaperone a 3775 +/- 186 3218 +/- 114 3953 +/- 59 3483 +/- 98 0.069263 -0.033245 -0.004841 0.0289523 0.0236638 TBCE tubulin-specific chaperone e 1108 +/- 25 1090 +/- 54 1222 +/- 53 1055 +/- 42 0.172167 -0.763513 -0.035533 0.7667842 0.0142401 TBL1X transducin (beta)-like 1X-linked 155 +/- 28 369 +/- 21 212 +/- 17 525 +/- 99 0.040271 0.0001457 0.033161 0.0033462 0.0739863 TBL3 transducin (beta)-like 3 579 +/- 36 464 +/- 23 541 +/- 36 472 +/- 24 -0.613143 -0.024171 -0.141556 0.017076 0.2097045 TCEAL4 transcription elongation factor A (SII)-like 4 8509 +/- 229 10384 +/- 384 8133 +/- 200 10329 +/- 553 -0.234371 0.0028996 0.013326 0.0979095 0.1382757 TCEAL8 transcription elongation factor A (SII)-like 8 2261 +/- 121 2724 +/- 87 2754 +/- 147 2815 +/- 38 0.006499 0.012366 0.698956 0.0146709 0.6065382 TCERG1 transcription elongation regulator 1 1003 +/- 111 1042 +/- 31 1253 +/- 60 1001 +/- 75 0.145381 0.7451163 -0.029947 0.5976185 0.2930007

S1- alphabetical list of aging-related genes.xls Page 32 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG TCF4 transcription factor 4 4852 +/- 158 4083 +/- 243 6014 +/- 463 4769 +/- 717 0.034252 -0.027614 -0.187716 0.3837346 0.9305804 TCF7L2 transcription factor 7-like 2 (T-cell specific, HMG-box) 1721 +/- 95 2204 +/- 120 1530 +/- 109 1712 +/- 113 -0.004094 0.0109185 0.276317 0.0005919 0.481373 TCFL5 transcription factor-like 5 (basic helix-loop-helix) 2337 +/- 183 1879 +/- 150 2593 +/- 180 1960 +/- 48 0.130726 -0.082168 -0.015834 0.29797 0.0273095 TDRD6 tudor domain containing 6 160 +/- 22 164 +/- 14 169 +/- 12 130 +/- 7 -0.652629 0.8808388 -0.026082 0.7248955 0.0643988 TEKT2 tektin 2 (testicular) 171 +/- 8 125 +/- 7 156 +/- 5 133 +/- 14 -0.371626 -0.001607 -0.191239 0.029531 0.6701408 TERF2 telomeric repeat binding factor 2 123 +/- 13 83 +/- 8 103 +/- 11 90 +/- 11 -0.519571 -0.027878 -0.404464 0.0020105 0.4186014 TEX10 testis expressed sequence 10 462 +/- 12 415 +/- 8 455 +/- 18 446 +/- 12 0.52937 -0.010267 -0.66446 0.019685 0.765926 TF transferrin 7019 +/- 1463 9974 +/- 1622 4189 +/- 354 6128 +/- 572 -0.012445 0.2061852 0.024171 0.1130597 0.0107645 TFG TRK-fused gene 5280 +/- 199 4443 +/- 99 4815 +/- 94 4186 +/- 147 -0.008725 -0.006472 -0.008783 0.00884 0.2431836 TFPI tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) 202 +/- 14 337 +/- 20 145 +/- 11 265 +/- 18 -0.000333 0.0003604 0.001002 0.0062585 0.0073557 TFPT TCF3 (E2A) fusion partner (in childhood Leukemia) 369 +/- 36 499 +/- 34 283 +/- 25 365 +/- 25 -0.000916 0.0243374 0.04248 0.0484472 0.0114551 TGFB3 transforming growth factor, beta 3 505 +/- 61 393 +/- 48 651 +/- 37 486 +/- 46 0.001231 -0.182678 -0.022851 0.548311 0.08447 TGM2 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) 141 +/- 8 82 +/- 19 137 +/- 20 132 +/- 28 0.264099 -0.026135 -0.900788 0.249611 0.8557524 TGOLN2 trans-golgi network protein 2 6539 +/- 384 7975 +/- 222 6466 +/- 326 7084 +/- 273 -0.178439 0.0119243 0.179939 0.002868 0.1434965 THAP11 THAP domain containing 11 1105 +/- 29 934 +/- 18 1207 +/- 52 1091 +/- 55 0.014562 -0.000838 -0.162464 0.0183226 0.0649289 THBS3 thrombospondin 3 546 +/- 53 445 +/- 55 780 +/- 44 597 +/- 55 0.002165 -0.214844 -0.03159 0.2819204 0.0687474 THEDC1 thioesterase domain containing 1 35 +/- 9 287 +/- 70 65 +/- 11 160 +/- 25 -0.294325 0.0152784 0.014874 0.000761 0.0131682 THNSL1 threonine synthase-like 1 (bacterial) 904 +/- 69 911 +/- 73 1077 +/- 20 1202 +/- 28 0.002795 0.9472046 0.007608 0.7074732 0.0448758 THRAP1 thyroid hormone receptor associated protein 1 2516 +/- 97 2385 +/- 106 2836 +/- 108 2482 +/- 58 0.047824 -0.383922 -0.021616 0.9182598 0.0677997 THUMPD3 THUMP domain containing 3 1272 +/- 40 1006 +/- 65 1229 +/- 30 921 +/- 79 -0.324468 -0.007846 -0.014491 0.030597 0.0080008 THY1 Thy-1 cell surface antigen 9156 +/- 962 5509 +/- 551 8393 +/- 478 6379 +/- 427 0.924806 -0.011092 -0.01192 0.0048733 0.0125296 TICAM1 toll-like receptor adaptor molecule 1 274 +/- 11 229 +/- 13 241 +/- 16 230 +/- 10 -0.221891 -0.025245 -0.55569 0.0035756 0.4334359 TIMM10 translocase of inner mitochondrial membrane 10 homolog (yeast) 1629 +/- 120 1047 +/- 77 1208 +/- 47 967 +/- 68 -0.01987 -0.003153 -0.021238 0.0054885 0.1440759 TIMM17A translocase of inner mitochondrial membrane 17 homolog A (yeast) 4599 +/- 193 5229 +/- 399 4545 +/- 37 5734 +/- 354 0.257734 0.1964322 0.027969 0.8853455 0.246689 TIMM17B translocase of inner mitochondrial membrane 17 homolog B (yeast) 797 +/- 21 693 +/- 17 734 +/- 44 746 +/- 42 -0.712147 -0.003378 0.8512 0.0013456 0.8624957 TIMM8A translocase of inner mitochondrial membrane 8 homolog A (yeast) 203 +/- 12 163 +/- 10 217 +/- 9 201 +/- 18 0.147768 -0.032671 -0.462692 0.0516373 0.6911374 TJP1 tight junction protein 1 (zona occludens 1) 881 +/- 94 1108 +/- 148 851 +/- 67 1056 +/- 37 -0.339328 0.2295937 0.029728 0.1657114 0.1157546 TJP2 tight junction protein 2 (zona occludens 2) 2291 +/- 204 3125 +/- 237 2144 +/- 139 2859 +/- 124 -0.146935 0.0240802 0.004053 0.0210485 0.0660687 TLK1 tousled-like kinase 1 109 +/- 15 104 +/- 11 155 +/- 15 108 +/- 11 0.088518 -0.817544 -0.029834 0.496871 0.0485331 TLN2 Talin 2 152 +/- 19 223 +/- 17 218 +/- 33 262 +/- 39 0.10167 0.0194107 0.416037 0.0162748 0.1306185 TM2D1 TM2 domain containing 1 743 +/- 38 713 +/- 50 856 +/- 54 690 +/- 41 0.295772 -0.642777 -0.037847 0.7145125 0.1792442 TM7SF2 transmembrane 7 superfamily member 2 1226 +/- 65 1455 +/- 59 1141 +/- 34 1258 +/- 73 -0.037433 0.0261398 0.201389 0.0392401 0.5412888 TM9SF2 transmembrane 9 superfamily member 2 1860 +/- 104 971 +/- 362 1861 +/- 69 746 +/- 349 -0.721198 -0.057369 -0.031588 0.4411309 0.162169 TM9SF3 transmembrane 9 superfamily member 3 965 +/- 31 1185 +/- 61 976 +/- 42 1008 +/- 62 -0.128617 0.0135836 0.683605 3.797E-05 0.2243917 TMBIM1 transmembrane BAX inhibitor motif containing 1 2080 +/- 216 2514 +/- 203 1362 +/- 54 1608 +/- 56 -0.000612 0.1734264 0.012063 0.0932183 0.1379688 TMBIM4 transmembrane BAX inhibitor motif containing 4 1977 +/- 143 2256 +/- 151 2223 +/- 85 1815 +/- 95 -0.970131 0.2105026 -0.011327 0.0921827 0.0409543 TMCC1 Transmembrane and coiled-coil domain family 1 100 +/- 14 167 +/- 12 175 +/- 18 218 +/- 27 0.008644 0.0045566 0.232236 0.002973 0.630778 TMCO3 transmembrane and coiled-coil domains 3 771 +/- 94 966 +/- 81 781 +/- 44 952 +/- 37 -0.694839 0.1469131 0.015859 0.0708804 0.1668245 TMED4 transmembrane emp24 protein transport domain containing 4 1134 +/- 55 1431 +/- 104 1087 +/- 50 1361 +/- 59 -0.045916 0.0364997 0.007201 0.0773 0.0247357 TMEM100 transmembrane protein 100 1126 +/- 85 764 +/- 92 810 +/- 93 482 +/- 73 -8.87E-06 -0.016282 -0.021697 0.0020981 0.0122112 TMEM106Ctransmembrane protein 106C 1970 +/- 98 2345 +/- 128 1940 +/- 209 2804 +/- 219 0.163504 0.0434133 0.019262 0.31285 0.2273303 TMEM107 transmembrane protein 107 159 +/- 12 208 +/- 5 187 +/- 9 204 +/- 18 0.319892 0.0060148 0.449575 0.0516834 0.946283 TMEM11 transmembrane protein 11 845 +/- 33 981 +/- 25 769 +/- 31 945 +/- 37 -0.003105 0.0089299 0.006195 0.1398978 0.1068125 TMEM116 transmembrane protein 116 119 +/- 13 198 +/- 25 90 +/- 10 142 +/- 15 -0.009295 0.0227208 0.023266 0.0091373 0.0654129

S1- alphabetical list of aging-related genes.xls Page 33 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG TMEM125 transmembrane protein 125 1877 +/- 348 2703 +/- 395 920 +/- 69 1320 +/- 139 -0.003931 0.1481536 0.042879 0.0392519 0.0982602 TMEM126Btransmembrane protein 126B 118 +/- 16 90 +/- 10 126 +/- 9 89 +/- 10 0.883673 -0.171889 -0.026306 0.2725305 0.0575722 TMEM132Btransmembrane protein 132B 1274 +/- 123 930 +/- 71 1051 +/- 67 730 +/- 54 -0.001972 -0.041705 -0.004742 0.2404027 0.1566965 TMEM132Etransmembrane protein 132E 138 +/- 10 118 +/- 18 232 +/- 19 159 +/- 19 0.003245 -0.347428 -0.023539 0.1210173 0.0709417 TMEM133 transmembrane protein 133 206 +/- 30 171 +/- 21 278 +/- 7 172 +/- 19 0.207571 -0.363118 -0.00354 0.2633708 0.0208326 TMEM14B transmembrane protein 14B 10702 +/- 355 8858 +/- 414 10426 +/- 173 9258 +/- 433 0.738658 -0.007202 -0.051729 0.000494 0.0016488 TMEM153 transmembrane protein 153 685 +/- 27 814 +/- 25 671 +/- 36 814 +/- 70 -0.95985 0.0060623 0.119132 0.0093753 0.288559 TMEM16D transmembrane protein 16D 287 +/- 24 220 +/- 14 135 +/- 14 141 +/- 14 -2.12E-05 -0.043747 0.778349 0.0263544 0.8800053 TMEM25 transmembrane protein 25 2517 +/- 184 2187 +/- 216 3846 +/- 199 3254 +/- 106 0.000106 -0.27173 -0.032156 0.2729187 0.1297675 TMEM30A transmembrane protein 30A 8163 +/- 210 7246 +/- 252 7449 +/- 446 7041 +/- 398 -0.142752 -0.019401 -0.511949 0.0284447 0.9216308 TMEM42 transmembrane protein 42 1365 +/- 45 1466 +/- 33 1244 +/- 38 1458 +/- 62 -0.040193 0.1013395 0.022844 0.3727597 0.1865494 TMEM48 transmembrane protein 48 109 +/- 18 137 +/- 12 135 +/- 6 164 +/- 4 0.066946 0.2324573 0.003928 0.3911182 0.1098339 TMEM50A transmembrane protein 50A 3252 +/- 47 2957 +/- 223 4076 +/- 139 3448 +/- 34 0.001072 -0.248078 -0.005475 0.4666802 0.025314 TMEM50B transmembrane protein 50B 10086 +/- 744 7418 +/- 951 7691 +/- 435 6249 +/- 882 -0.010509 -0.053133 -0.193944 0.0045491 0.0299147 TMEM51 transmembrane protein 51 459 +/- 34 555 +/- 15 354 +/- 21 396 +/- 28 -0.000141 0.0384605 0.277148 0.1356155 0.5956454 TMEM57 transmembrane protein 57 604 +/- 27 705 +/- 15 723 +/- 33 725 +/- 8 0.046032 0.0126412 0.945545 0.0242234 0.8980879 TMEM62 transmembrane protein 62 272 +/- 20 338 +/- 21 254 +/- 9 273 +/- 20 -0.024941 0.0504131 0.433539 0.0665573 0.7988088 TMEM64 Transmembrane protein 64 338 +/- 27 289 +/- 14 377 +/- 21 301 +/- 16 0.17955 -0.146266 -0.020925 0.2949438 0.0073293 TMEM76 transmembrane protein 76 3761 +/- 96 3217 +/- 173 4452 +/- 182 4256 +/- 291 0.000104 -0.025905 -0.587721 0.0215696 0.3439686 TMEM80 transmembrane protein 80 995 +/- 50 1270 +/- 42 785 +/- 32 942 +/- 27 -1.15E-05 0.0019743 0.00455 0.0025206 0.0233829 TMEM87B Transmembrane protein 87B 96 +/- 30 190 +/- 26 87 +/- 22 198 +/- 10 -0.869969 0.0397936 0.002631 0.1807296 0.0209088 TMEM91 transmembrane protein 91 837 +/- 63 517 +/- 43 501 +/- 26 438 +/- 36 -0.003916 -0.00249 -0.196617 0.0104634 0.54782 TMEM93 transmembrane protein 93 2312 +/- 100 1990 +/- 126 2258 +/- 82 1965 +/- 87 -0.971757 -0.075388 -0.037945 0.1866277 0.0607565 TMEM97 transmembrane protein 97 1042 +/- 36 1322 +/- 88 914 +/- 45 1135 +/- 30 -0.00711 0.0231497 0.003205 0.0174164 0.0196386 TMEPAI transmembrane, prostate androgen induced RNA 899 +/- 154 699 +/- 73 772 +/- 55 601 +/- 44 -0.256243 -0.277782 -0.038059 0.1698743 0.1604555 TMF1 TATA element modulatory factor 1 2030 +/- 133 1560 +/- 130 2148 +/- 200 1684 +/- 181 0.247176 -0.029535 -0.118838 0.0634494 0.0932345 TMTC4 transmembrane and tetratricopeptide repeat containing 4 1389 +/- 212 2022 +/- 276 751 +/- 81 984 +/- 63 -0.000888 0.1009476 0.049565 0.1159551 0.1834717 TncRNA trophoblast-derived noncoding RNA 1717 +/- 180 2482 +/- 183 1618 +/- 126 1986 +/- 125 -0.103308 0.0137655 0.068035 0.0034843 0.0251874 TNFAIP6 tumor necrosis factor, alpha-induced protein 6 258 +/- 31 467 +/- 111 161 +/- 13 251 +/- 29 -0.02623 0.1228848 0.031792 0.012802 0.0430429 TNFAIP8L1tumor necrosis factor, alpha-induced protein 8-like 1 625 +/- 37 647 +/- 29 658 +/- 40 775 +/- 14 0.03887 0.6471604 0.032229 0.8891392 0.0755139 TNFRSF19tumor necrosis factor receptor superfamily, member 19 113 +/- 17 70 +/- 3 136 +/- 13 101 +/- 8 0.022313 -0.052431 -0.056666 0.0367617 0.1181179 TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 626 +/- 50 900 +/- 75 508 +/- 50 663 +/- 50 -0.023771 0.0144421 0.056884 0.006527 0.1341425 TNFSF13Btumor necrosis factor (ligand) superfamily, member 13b 429 +/- 38 458 +/- 44 416 +/- 30 552 +/- 32 0.229499 0.6342725 0.013214 0.3676074 0.0230484 TNIP2 TNFAIP3 interacting protein 2 474 +/- 48 575 +/- 35 439 +/- 19 505 +/- 20 -0.129579 0.124973 0.042061 0.3179682 0.042892 TNRC4 trinucleotide repeat containing 4 4534 +/- 210 3677 +/- 260 5558 +/- 185 4760 +/- 252 0.000551 -0.029148 -0.035167 0.0107826 0.1256802 TNRC9 trinucleotide repeat containing 9 230 +/- 31 204 +/- 19 217 +/- 25 147 +/- 15 -0.153798 -0.488732 -0.042514 0.4769329 0.0994167 TNS3 tensin 3 5926 +/- 520 7061 +/- 361 4964 +/- 174 6047 +/- 403 -0.002609 0.1067943 0.052473 0.3937513 0.3502093 TNXA tenascin XA pseudogene 497 +/- 80 915 +/- 160 295 +/- 39 476 +/- 26 -0.012749 0.0506152 0.004207 0.0098381 0.0079701 TOLLIP toll interacting protein 3373 +/- 197 2715 +/- 128 3379 +/- 182 2936 +/- 92 0.371372 -0.021608 -0.065072 0.007421 0.1245387 TOM1L1 Target of myb1-like 1 (chicken) 114 +/- 13 121 +/- 6 130 +/- 12 171 +/- 11 0.025777 0.6318576 0.030309 0.6264392 0.0957465 TOMM40L translocase of outer mitochondrial membrane 40 homolog-like (yeast) 1176 +/- 55 1011 +/- 26 1124 +/- 72 1062 +/- 68 -0.862307 -0.029641 -0.5499 0.0120262 0.9358822 TOP1MT topoisomerase (DNA) I, mitochondrial 381 +/- 24 369 +/- 14 378 +/- 17 328 +/- 12 -0.086456 -0.659272 -0.038398 0.8367292 0.0365229 TOR1AIP1 torsin A interacting protein 1 848 +/- 70 1089 +/- 39 1211 +/- 42 1195 +/- 20 0.002503 0.0176232 -0.739001 0.0164243 0.7918809 TOR1B torsin family 1, member B (torsin B) 824 +/- 61 605 +/- 42 797 +/- 27 668 +/- 33 0.758247 -0.015886 -0.016497 0.0286671 0.0185479

S1- alphabetical list of aging-related genes.xls Page 34 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG TOR3A torsin family 3, member A 298 +/- 17 220 +/- 15 308 +/- 11 206 +/- 13 -0.734421 -0.006224 -0.000303 0.0676836 0.0017675 TP53BP2 tumor protein p53 binding protein, 2 3969 +/- 331 4293 +/- 320 4425 +/- 200 3879 +/- 120 0.304194 0.496883 -0.047499 0.2495646 0.298379 TP53RK TP53 regulating kinase 189 +/- 12 227 +/- 11 170 +/- 2 221 +/- 13 -0.156343 0.0375501 0.014507 0.0098446 5.072E-06 TPD52 tumor protein D52 200 +/- 13 258 +/- 21 346 +/- 17 380 +/- 12 1.36E-06 0.0473867 0.132678 0.1577976 0.2435336 TPM1 Tropomyosin 1 (alpha) 396 +/- 71 679 +/- 70 626 +/- 100 856 +/- 98 0.000756 0.0180423 0.135836 0.0174101 0.0760796 TPP1 tripeptidyl peptidase I 1777 +/- 184 2252 +/- 226 1825 +/- 88 2210 +/- 122 0.745375 0.1350991 0.03549 0.1099582 0.072628 TPT1 tumor protein, translationally-controlled 1 440 +/- 33 330 +/- 27 527 +/- 42 469 +/- 48 0.001767 -0.028535 -0.385139 0.0312002 0.4178308 TRA2A transformer-2 alpha 1956 +/- 84 1639 +/- 102 2458 +/- 104 1919 +/- 80 0.000253 -0.03851 -0.002777 0.3163716 0.022919 TRAF3 TNF receptor-associated factor 3 1143 +/- 89 1002 +/- 66 1473 +/- 46 1224 +/- 60 0.001402 -0.233136 -0.011177 0.193721 0.0476289 TRAFD1 TRAF-type zinc finger domain containing 1 1185 +/- 77 1021 +/- 41 913 +/- 16 812 +/- 32 -0.000443 -0.098765 -0.028756 0.2705776 0.0476544 TRAK1 trafficking protein, kinesin binding 1 827 +/- 63 630 +/- 58 1039 +/- 46 885 +/- 23 0.000187 -0.044996 -0.018605 0.0166329 0.0124228 TRAK2 trafficking protein, kinesin binding 2 2695 +/- 155 3432 +/- 218 2515 +/- 153 2941 +/- 67 -0.029831 0.0224005 0.039325 0.0113992 0.044261 TRAM2 translocation associated membrane protein 2 242 +/- 13 139 +/- 24 223 +/- 20 171 +/- 18 0.890633 -0.005996 -0.081752 0.0558812 0.1085386 TRAPPC2 trafficking protein particle complex 2 1738 +/- 51 1929 +/- 62 1792 +/- 47 1967 +/- 42 0.376883 0.0389203 0.020946 0.0442794 0.0368327 TRAPPC5 trafficking protein particle complex 5 2581 +/- 63 2125 +/- 116 2463 +/- 162 2219 +/- 88 -0.873413 -0.009296 -0.223797 0.0244116 0.7278398 TREX2 three prime repair exonuclease 2 95 +/- 10 117 +/- 10 117 +/- 14 160 +/- 11 0.001483 0.1529645 0.039352 0.6087976 0.2772861 TRIM2 tripartite motif-containing 2 134 +/- 28 96 +/- 14 147 +/- 12 92 +/- 9 0.9229 -0.251682 -0.00594 0.3928277 0.0230353 TRIM37 tripartite motif-containing 37 6886 +/- 322 6344 +/- 360 6293 +/- 202 6895 +/- 149 -0.639424 -0.288018 0.041014 0.093437 0.1241056 TRIM46 tripartite motif-containing 46 886 +/- 40 715 +/- 65 1011 +/- 67 787 +/- 42 0.07113 -0.052704 -0.021733 0.0189775 0.0721362 TRIM72 tripartite motif-containing 72 123 +/- 14 108 +/- 18 125 +/- 21 67 +/- 8 -0.29077 -0.549455 -0.039728 0.8562585 0.0466648 TRIO Triple functional domain (PTPRF interacting) 232 +/- 36 388 +/- 49 416 +/- 22 423 +/- 36 0.012866 0.0303455 0.867579 0.15146 0.6239491 TRIP6 thyroid hormone receptor interactor 6 448 +/- 39 566 +/- 21 435 +/- 17 459 +/- 14 -0.045033 0.0308227 0.305349 0.1291424 0.8550607 TRMT12 tRNA methyltranferase 12 homolog (S. cerevisiae) 506 +/- 22 617 +/- 34 498 +/- 40 575 +/- 20 -0.364153 0.0234104 0.12859 0.3505438 0.4556509 TRPM7 transient receptor potential cation channel, subfamily M, member 7 356 +/- 40 334 +/- 21 378 +/- 30 285 +/- 20 -0.870857 -0.62917 -0.031435 0.9676145 0.2030345 TRUB2 TruB pseudouridine (psi) synthase homolog 2 (E. coli) 855 +/- 93 1100 +/- 56 795 +/- 42 1011 +/- 37 -0.134749 0.0521423 0.003953 0.1284202 0.1004199 TSC22D2 TSC22 domain family, member 2 2203 +/- 83 2607 +/- 109 2346 +/- 100 2554 +/- 91 0.222684 0.0158673 0.157063 0.0651624 0.5944183 TSEN34 tRNA splicing endonuclease 34 homolog (S. cerevisiae) 991 +/- 52 1198 +/- 77 784 +/- 49 985 +/- 39 -0.00045 0.0529505 0.010934 0.3530183 0.0224772 TSGA2 testis specific A2 homolog (mouse) 658 +/- 150 274 +/- 41 370 +/- 40 239 +/- 30 -0.149288 -0.049774 -0.027797 0.0441117 0.1137503 TSPAN3 tetraspanin 3 4001 +/- 86 3629 +/- 136 3966 +/- 66 3699 +/- 96 0.92958 -0.047448 -0.05388 0.0498443 0.2401377 TSPYL3 TSPY-like 3 (pseudogene) 716 +/- 53 638 +/- 63 867 +/- 80 645 +/- 47 0.049766 -0.361301 -0.04429 0.4325361 0.1425855 TTBK2 Tau tubulin kinase 2 1287 +/- 128 1604 +/- 88 1035 +/- 99 1388 +/- 79 -8.85E-05 0.0719335 0.02098 0.0493838 0.0120741 TTC19 tetratricopeptide repeat domain 19 2924 +/- 101 1559 +/- 359 3189 +/- 138 1757 +/- 528 0.051201 -0.011318 -0.051447 0.0656807 0.1680265 TTC27 tetratricopeptide repeat domain 27 442 +/- 31 538 +/- 25 493 +/- 26 422 +/- 31 -0.641755 0.0399541 -0.120361 0.1612213 0.2185023 TTF1 transcription termination factor, RNA polymerase I 700 +/- 25 781 +/- 23 793 +/- 51 789 +/- 50 0.135399 0.0398668 -0.961344 0.0755174 0.7383038 TTLL4 tubulin tyrosine ligase-like family, member 4 380 +/- 22 458 +/- 23 287 +/- 14 309 +/- 11 -0.000194 0.0373522 0.234891 0.0031157 0.3031171 TUBB tubulin, beta 14019 +/- 320 12891 +/- 352 13789 +/- 333 13992 +/- 673 0.502382 -0.039425 0.795458 0.0112813 0.4504876 TUBB2A tubulin, beta 2A 32505 +/- 1058 29201 +/- 789 30330 +/- 702 30704 +/- 944 -0.647763 -0.033009 0.759243 0.0168572 0.6398083 TUBB2B tubulin, beta 2B 3672 +/- 237 3414 +/- 308 3355 +/- 175 2679 +/- 191 -0.021638 -0.521948 -0.029004 0.7455158 0.0914478 TUBGCP2 tubulin, gamma complex associated protein 2 1011 +/- 12 1088 +/- 28 991 +/- 31 1088 +/- 35 -0.688769 0.0396274 0.069901 0.0187537 0.1460281 TUBGCP5 tubulin, gamma complex associated protein 5 697 +/- 50 478 +/- 121 647 +/- 36 371 +/- 78 -0.185068 -0.139882 -0.020017 0.3173034 0.0374281 TUFM Tu translation elongation factor, mitochondrial 1075 +/- 75 895 +/- 60 995 +/- 31 811 +/- 30 -0.058157 -0.091891 -0.002054 0.0799602 0.055396 TUFT1 tuftelin 1 796 +/- 100 1318 +/- 115 650 +/- 8 921 +/- 37 -0.01834 0.0067359 0.001405 0.0112191 0.03166 TUSC1 tumor suppressor candidate 1 965 +/- 92 888 +/- 78 1306 +/- 67 1105 +/- 34 0.012341 -0.537009 -0.031015 0.4691942 0.0926691 TUSC3 tumor suppressor candidate 3 593 +/- 43 437 +/- 30 743 +/- 35 545 +/- 36 0.00246 -0.015208 -0.003574 0.0268112 0.0789955

S1- alphabetical list of aging-related genes.xls Page 35 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG TUSC4 tumor suppressor candidate 4 680 +/- 15 597 +/- 17 608 +/- 25 633 +/- 24 -0.361644 -0.004189 0.477032 0.0017272 0.8305597 TWSG1 twisted gastrulation homolog 1 (Drosophila) 237 +/- 29 361 +/- 36 277 +/- 24 370 +/- 42 0.540404 0.0239225 0.098445 0.0549182 0.3838287 TXNDC10 thioredoxin domain containing 10 1152 +/- 79 1252 +/- 69 1427 +/- 33 1248 +/- 63 0.091054 0.366338 -0.046452 0.6779952 0.1847538 TXNDC4 thioredoxin domain containing 4 (endoplasmic reticulum) 1246 +/- 67 1050 +/- 62 1153 +/- 88 920 +/- 29 -0.037892 -0.057809 -0.044765 0.3896292 0.2612776 TXNDC9 thioredoxin domain containing 9 1042 +/- 34 872 +/- 30 979 +/- 44 900 +/- 61 -0.755935 -0.003787 -0.325893 0.0694099 0.2750064 TXNL1 thioredoxin-like 1 2413 +/- 328 2952 +/- 186 3020 +/- 129 3692 +/- 218 0.011617 0.1909979 0.03449 0.2960004 0.0595136 TXNL5 thioredoxin-like 5 875 +/- 37 924 +/- 31 895 +/- 46 1038 +/- 34 0.124208 0.3406681 0.033641 0.2201285 0.0538183 TYROBP TYRO protein tyrosine kinase binding protein 1055 +/- 57 1424 +/- 146 717 +/- 24 899 +/- 63 -0.00327 0.0533008 0.041326 0.0060139 0.1496422 UBAP2L ubiquitin associated protein 2-like 1045 +/- 48 1221 +/- 34 950 +/- 19 1068 +/- 43 -0.002699 0.0145306 0.047736 0.033027 0.2012221 UBC ubiquitin C 6672 +/- 739 5772 +/- 426 7116 +/- 734 5191 +/- 278 -0.856537 -0.321669 -0.047247 0.4124151 0.1821162 UBE1 ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing) 6200 +/- 205 6514 +/- 169 6473 +/- 145 7267 +/- 148 0.00557 0.2658432 0.004142 0.8529343 0.0520709 UBE1DC1 ubiquitin-activating enzyme E1-domain containing 1 944 +/- 81 433 +/- 60 1060 +/- 122 436 +/- 35 0.016502 -0.00065 -0.00298 0.0148963 0.0210426 UBE2G1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 643 +/- 51 771 +/- 63 502 +/- 12 695 +/- 17 -0.021358 0.150032 2.34E-05 0.3163649 0.0032084 UBE2H ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 3587 +/- 143 4508 +/- 50 3408 +/- 125 4376 +/- 229 -0.282662 0.0007993 0.00919 0.0016336 0.0835148 UBE2J1 ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) 447 +/- 25 412 +/- 15 587 +/- 24 472 +/- 9 0.001425 -0.256295 -0.003566 0.1363247 0.0170116 UBE2J2 ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) 433 +/- 20 329 +/- 22 433 +/- 21 410 +/- 38 0.112513 -0.005392 -0.608341 0.0326802 0.9463236 UBE2L6 ubiquitin-conjugating enzyme E2L 6 690 +/- 43 965 +/- 37 708 +/- 47 941 +/- 37 -0.890367 0.0006893 0.003762 0.0040887 0.0059346 UBE2V2 ubiquitin-conjugating enzyme E2 variant 2 1294 +/- 48 1322 +/- 90 1405 +/- 72 1126 +/- 68 -0.846888 0.7892999 -0.020027 0.9176096 0.1203577 UBE3A ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndr 6737 +/- 339 5705 +/- 371 5837 +/- 154 5224 +/- 81 -0.046238 -0.067394 -0.008728 0.1696783 0.0658193 UBR1 ubiquitin protein ligase E3 component n-recognin 1 655 +/- 48 599 +/- 38 837 +/- 25 692 +/- 31 0.002617 -0.387825 -0.006326 0.393552 0.0068199 UBR2 ubiquitin protein ligase E3 component n-recognin 2 1287 +/- 52 1516 +/- 35 1269 +/- 37 1337 +/- 21 -0.090797 0.0056816 0.154154 0.004776 0.5701314 UCK1 uridine-cytidine kinase 1 1401 +/- 163 1218 +/- 106 1046 +/- 54 836 +/- 69 -0.004824 -0.371207 -0.04278 0.4779683 0.0327209 UCN urocortin 399 +/- 28 387 +/- 22 380 +/- 17 317 +/- 13 -0.110004 -0.741044 -0.017921 0.9887089 0.1015131 UFD1L ubiquitin fusion degradation 1 like (yeast) 1115 +/- 186 1832 +/- 170 1206 +/- 182 1617 +/- 52 -0.884698 0.0175615 0.073879 0.0244533 0.1332247 UGCG UDP-glucose ceramide glucosyltransferase 1454 +/- 63 1024 +/- 64 1638 +/- 69 1487 +/- 121 0.016063 -0.000733 -0.318254 0.0010664 0.4295965 UNC13C unc-13 homolog C (C. elegans) 555 +/- 100 536 +/- 112 1134 +/- 113 1508 +/- 113 6.49E-05 -0.904622 0.044441 0.9489766 0.1532116 UNC45A unc-45 homolog A (C. elegans) 863 +/- 83 692 +/- 25 923 +/- 40 731 +/- 59 0.200837 -0.096748 -0.029506 0.3684818 0.4271125 UNC84A unc-84 homolog A (C. elegans) 302 +/- 15 412 +/- 31 411 +/- 55 383 +/- 30 0.290753 0.0148922 -0.669887 0.1154254 0.8653508 UNC93B1 unc-93 homolog B1 (C. elegans) 102 +/- 18 164 +/- 21 91 +/- 9 122 +/- 14 -0.191111 0.0498508 0.105323 0.0264692 0.0892444 UQCRC1 ubiquinol-cytochrome c reductase core protein I 5480 +/- 38 5588 +/- 258 5191 +/- 86 5734 +/- 178 -0.468081 0.695535 0.034439 0.4736981 0.1237464 USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) 1553 +/- 103 1788 +/- 135 1582 +/- 72 1981 +/- 114 0.491904 0.1987562 0.021016 0.2845093 0.001748 USP15 ubiquitin specific peptidase 15 2158 +/- 53 1639 +/- 107 2009 +/- 51 1697 +/- 92 -0.29659 -0.003135 -0.023253 0.0583221 0.3244356 USP31 ubiquitin specific peptidase 31 360 +/- 19 436 +/- 27 499 +/- 36 687 +/- 45 0.000805 0.0458499 0.011486 0.1335057 0.1219157 USP4 ubiquitin specific peptidase 4 (proto-oncogene) 2228 +/- 74 1806 +/- 34 2258 +/- 99 1973 +/- 65 0.074557 -0.001324 -0.041874 0.0153513 0.3543365 USP53 ubiquitin specific peptidase 53 346 +/- 23 476 +/- 40 443 +/- 44 496 +/- 19 0.078882 0.0220377 0.304397 0.1497631 0.6399372 UTP11L UTP11-like, U3 small nucleolar ribonucleoprotein, (yeast) 1063 +/- 52 748 +/- 78 1102 +/- 32 926 +/- 140 0.076356 -0.009004 -0.282294 0.0156669 0.2929523 UTP14C UTP14, U3 small nucleolar ribonucleoprotein, homolog C (yeast) 1372 +/- 46 1224 +/- 51 1428 +/- 32 1176 +/- 49 0.546197 -0.056119 -0.00361 0.2103452 0.0278904 UTP18 UTP18, small subunit (SSU) processome component, homolog (yeast) 599 +/- 58 413 +/- 38 673 +/- 50 479 +/- 70 0.043722 -0.026658 -0.055462 0.1362504 0.3656743 VAPA VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa 11082 +/- 381 9798 +/- 151 10132 +/- 197 8992 +/- 317 -0.01107 -0.018084 -0.018774 0.1149009 0.004102 VASH1 Vasohibin 1 535 +/- 16 744 +/- 63 1011 +/- 90 999 +/- 53 2.52E-05 0.0198306 -0.908355 0.071398 0.9152143 VDP vesicle docking protein p115 333 +/- 23 487 +/- 24 387 +/- 27 440 +/- 22 0.478245 0.0008437 0.162965 0.0003588 0.0685504 VEGF vascular endothelial growth factor 2713 +/- 90 2084 +/- 104 2439 +/- 134 2150 +/- 54 -0.407872 -0.001085 -0.088854 0.0081142 0.1448131 VEZT vezatin, adherens junctions transmembrane protein 486 +/- 18 420 +/- 10 474 +/- 38 465 +/- 24 0.627724 -0.01232 -0.832441 0.0080561 0.5767741 VMO1 vitelline membrane outer layer 1 homolog (chicken) 691 +/- 50 1261 +/- 202 543 +/- 79 843 +/- 117 -0.002328 0.0360849 0.069831 0.0180857 0.022593

S1- alphabetical list of aging-related genes.xls Page 36 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG VPS13B vacuolar protein sorting 13B (yeast) 993 +/- 26 852 +/- 41 949 +/- 24 938 +/- 47 0.843752 -0.018654 -0.831078 0.1952555 0.5089745 VPS13C Vacuolar protein sorting 13 homolog C (S. cerevisiae) 1099 +/- 47 1286 +/- 45 1139 +/- 97 1352 +/- 95 0.438574 0.0175363 0.149298 0.0306072 0.5404622 VPS37A vacuolar protein sorting 37 homolog A (S. cerevisiae) 350 +/- 19 271 +/- 22 344 +/- 18 318 +/- 14 0.339961 -0.022864 -0.295475 0.0472386 0.0712447 VPS39 vacuolar protein sorting 39 (yeast) 1307 +/- 34 1456 +/- 46 1449 +/- 64 1330 +/- 79 0.668535 0.0282843 -0.273342 0.0310715 0.5496363 VPS52 vacuolar protein sorting 52 (S. cerevisiae) 2096 +/- 88 2181 +/- 130 2255 +/- 63 2679 +/- 158 0.004678 0.6022707 0.052527 0.8677483 0.0234175 VPS53 vacuolar protein sorting 53 (S. cerevisiae) 146 +/- 12 263 +/- 30 186 +/- 19 252 +/- 27 0.499944 0.0089525 0.084191 0.0156657 0.1320008 VTI1B vesicle transport through interaction with t-SNAREs homolog 1B (yeast) 3503 +/- 235 3273 +/- 106 2823 +/- 159 3297 +/- 104 -0.120362 -0.403886 0.03647 0.2761022 0.0257349 WAC WW domain containing adaptor with coiled-coil 3946 +/- 99 4045 +/- 77 3700 +/- 73 4022 +/- 31 -0.008381 0.4501341 0.005534 0.3428936 0.03231 WAPAL wings apart-like homolog (Drosophila) 1297 +/- 39 1452 +/- 53 1324 +/- 21 1373 +/- 65 -0.928265 0.0422755 0.509868 0.0426606 0.6191482 WAS Wiskott-Aldrich syndrome (eczema-thrombocytopenia) 214 +/- 11 177 +/- 8 239 +/- 10 176 +/- 17 0.227253 -0.021343 -0.016449 0.1170599 0.003993 WASPIP Wiskott-Aldrich syndrome protein interacting protein 453 +/- 53 836 +/- 159 339 +/- 48 483 +/- 31 -0.031369 0.0615464 0.036627 0.0056073 0.0362281 WBP1 WW domain binding protein 1 1823 +/- 116 2506 +/- 129 1839 +/- 149 2251 +/- 134 -0.111839 0.0028595 0.069745 0.0103896 0.1447055 WBSCR17 Williams-Beuren syndrome chromosome region 17 3846 +/- 222 2759 +/- 329 4476 +/- 273 3946 +/- 239 0.003811 -0.023533 -0.178057 0.0095031 0.5398113 WDFY1 WD repeat and FYVE domain containing 1 1082 +/- 175 808 +/- 190 857 +/- 69 579 +/- 68 -0.015588 -0.316252 -0.018632 0.8156256 0.2181605 WDR20 WD repeat domain 20 126 +/- 15 179 +/- 17 107 +/- 16 129 +/- 10 -0.02678 0.039402 0.281023 0.0059351 0.4156628 WDR23 WD repeat domain 23 428 +/- 44 312 +/- 19 348 +/- 33 318 +/- 26 -0.274186 -0.046415 -0.501504 0.0755089 0.6240834 WDR25 WD repeat domain 25 360 +/- 15 348 +/- 18 272 +/- 4 365 +/- 15 -0.2405 -0.6193 0.002078 0.272477 0.0034098 WDR36 WD repeat domain 36 210 +/- 17 205 +/- 18 260 +/- 9 213 +/- 15 0.121961 -0.841738 -0.037096 0.9362799 0.0466436 WDR47 WD repeat domain 47 4038 +/- 157 3797 +/- 170 4306 +/- 111 3939 +/- 111 0.055136 -0.322562 -0.045221 0.1430744 0.1724067 WDR57 WD repeat domain 57 (U5 snRNP specific) 959 +/- 34 1105 +/- 11 958 +/- 17 1063 +/- 49 -0.619116 0.0063689 0.097725 0.0200081 0.4501393 WDR71 WD repeat domain 71 1094 +/- 63 1355 +/- 56 978 +/- 43 1086 +/- 116 -0.007047 0.0110987 0.422752 0.0963653 0.7656435 WDR73 WD repeat domain 73 236 +/- 7 291 +/- 13 265 +/- 22 258 +/- 15 -0.882034 0.0056071 -0.775932 0.0124797 0.6874124 WDR74 WD repeat domain 74 744 +/- 14 687 +/- 26 664 +/- 37 566 +/- 24 -0.004433 -0.095725 -0.05306 0.2019727 0.1043173 WEE1 WEE1 homolog (S. pombe) 156 +/- 15 113 +/- 11 162 +/- 19 156 +/- 13 0.080558 -0.043433 -0.792523 0.0274297 0.3148447 WHDC1 WAS protein homology region 2 domain containing 1 276 +/- 18 251 +/- 22 322 +/- 19 263 +/- 13 0.001236 -0.402533 -0.033976 0.2380032 0.061158 WHDC1L1 WAS protein homology region 2 domain containing 1-like 1 103 +/- 9 161 +/- 16 133 +/- 14 116 +/- 11 -0.905068 0.0147173 -0.368477 0.0166033 0.1508871 WHSC1 Wolf-Hirschhorn syndrome candidate 1 291 +/- 18 359 +/- 21 296 +/- 30 337 +/- 23 -0.629015 0.0335982 0.30691 0.0574177 0.2165234 WNK1 WNK lysine deficient protein kinase 1 169 +/- 12 233 +/- 22 147 +/- 11 191 +/- 17 -0.025793 0.0311083 0.069392 0.0020538 0.0029629 WNT5A wingless-type MMTV integration site family, member 5A 485 +/- 51 763 +/- 107 574 +/- 90 877 +/- 81 0.234303 0.0504035 0.034137 0.0055733 0.0175677 WTAP Wilms tumor 1 associated protein 519 +/- 31 710 +/- 21 683 +/- 41 771 +/- 66 0.012206 0.0006447 0.292143 0.0003647 0.0433612 WWOX WW domain containing oxidoreductase 524 +/- 32 696 +/- 66 457 +/- 17 483 +/- 57 -0.018561 0.0511504 0.684042 0.0066884 0.8592822 WWP1 WW domain containing E3 ubiquitin protein ligase 1 1658 +/- 161 2181 +/- 165 1410 +/- 176 1562 +/- 24 -0.003309 0.0466357 0.43057 0.0263697 0.3583863 XKR4 XK, Kell blood group complex subunit-related family, member 4 1695 +/- 77 1381 +/- 64 1756 +/- 52 1344 +/- 38 0.813186 -0.010927 -0.000144 0.0064131 0.0078778 XPNPEP1 X-prolyl aminopeptidase (aminopeptidase P) 1, soluble 1226 +/- 73 1063 +/- 32 1269 +/- 56 1109 +/- 24 0.337722 -0.080738 -0.035698 0.0328858 0.0767639 XPO5 exportin 5 429 +/- 36 342 +/- 18 461 +/- 21 383 +/- 24 0.284697 -0.066394 -0.040657 0.2169755 0.0262118 XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-brea 1545 +/- 90 1864 +/- 59 1424 +/- 33 1774 +/- 97 -0.034978 0.0164915 0.019361 0.2486474 0.11865 XTP3TPA XTP3-transactivated protein A 1242 +/- 57 1263 +/- 36 1182 +/- 54 1352 +/- 33 0.976487 0.7683885 0.027719 0.5634529 0.0450114 YIF1A Yip1 interacting factor homolog A (S. cerevisiae) 1268 +/- 101 1457 +/- 104 804 +/- 64 1043 +/- 52 -0.000185 0.2207861 0.017631 0.1303022 0.2988299 YIF1B Yip1 interacting factor homolog B (S. cerevisiae) 521 +/- 34 657 +/- 43 514 +/- 32 631 +/- 45 -0.173589 0.0319616 0.067484 0.012497 0.006137 YIPF5 Yip1 domain family, member 5 2776 +/- 72 2388 +/- 136 2883 +/- 102 2798 +/- 74 0.02751 -0.037028 -0.516929 0.0084887 0.633725 YME1L1 YME1-like 1 (S. cerevisiae) 3560 +/- 193 4334 +/- 244 3653 +/- 205 4168 +/- 128 -0.80019 0.0331531 0.065747 0.3551589 0.3036552 YRDC yrdC domain containing (E. coli) 396 +/- 15 305 +/- 14 466 +/- 9 394 +/- 30 0.001004 -0.001409 -0.075638 0.0027356 0.4238058 YTHDC1 YTH domain containing 1 3734 +/- 134 4179 +/- 118 3676 +/- 128 4478 +/- 290 0.634002 0.0314959 0.048117 0.003624 0.1540466 YTHDF2 YTH domain family, member 2 2059 +/- 97 1665 +/- 58 2492 +/- 66 2178 +/- 98 0.00012 -0.007866 -0.031414 0.1128308 0.432849

S1- alphabetical list of aging-related genes.xls Page 37 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG YTHDF3 YTH domain family, member 3 3848 +/- 269 3599 +/- 115 3721 +/- 144 3177 +/- 92 -0.217782 -0.424114 -0.012373 0.6292132 0.0622836 ZBTB24 zinc finger and BTB domain containing 24 626 +/- 63 454 +/- 23 608 +/- 38 435 +/- 38 -0.536329 -0.039583 -0.010113 0.1877211 0.1509857 ZBTB26 zinc finger and BTB domain containing 26 380 +/- 28 505 +/- 16 502 +/- 29 427 +/- 37 0.501763 0.0047141 -0.148916 0.0009437 0.4279081 ZBTB7A zinc finger and BTB domain containing 7A 1920 +/- 64 1641 +/- 79 2555 +/- 279 2188 +/- 127 0.001221 -0.021473 -0.27096 0.1108262 0.5977751 ZBTB7C zinc finger and BTB domain containing 7C 448 +/- 21 348 +/- 43 483 +/- 41 358 +/- 31 0.221755 -0.074596 -0.037872 0.0804206 0.1577621 ZC3H13 Zinc finger CCCH-type containing 13 2082 +/- 90 1605 +/- 162 1861 +/- 75 1362 +/- 193 -0.012616 -0.0334 -0.05896 0.0151073 0.0145363 ZC3H14 zinc finger CCCH-type containing 14 1650 +/- 138 1245 +/- 70 1782 +/- 55 1603 +/- 67 0.006636 -0.033219 -0.072998 0.0842893 0.4758872 ZC3H5 zinc finger CCCH-type containing 5 511 +/- 50 651 +/- 39 464 +/- 35 503 +/- 41 -0.035672 0.0536802 0.490227 0.1864741 0.5588391 ZCCHC10 zinc finger, CCHC domain containing 10 274 +/- 13 341 +/- 16 327 +/- 16 328 +/- 20 0.207084 0.0091703 0.953354 0.0262749 0.5930633 ZCCHC6 zinc finger, CCHC domain containing 6 609 +/- 30 750 +/- 31 578 +/- 31 641 +/- 42 -0.152995 0.0086991 0.260145 0.0085913 0.1891606 ZCCHC7 Zinc finger, CCHC domain containing 7 91 +/- 10 151 +/- 6 114 +/- 11 145 +/- 19 0.362943 0.0009984 0.205209 0.0324606 0.9378469 ZF HCF-binding transcription factor Zhangfei 481 +/- 22 590 +/- 26 608 +/- 20 692 +/- 20 0.000708 0.0098783 0.016805 0.0079603 0.3500007 ZFP90 zinc finger protein 90 homolog (mouse) 3528 +/- 279 4260 +/- 161 3870 +/- 345 4323 +/- 157 0.025622 0.0525751 0.270959 0.0915682 0.4421446 ZKSCAN1 zinc finger with KRAB and SCAN domains 1 1152 +/- 95 1766 +/- 191 984 +/- 18 1287 +/- 34 -0.037284 0.0226658 0.000206 0.0075827 0.0004425 ZMPSTE24zinc metallopeptidase (STE24 homolog, yeast) 681 +/- 23 630 +/- 24 787 +/- 36 633 +/- 19 0.116857 -0.153945 -0.005707 0.3891191 0.1089935 ZNF131 Zinc finger protein 131 (clone pHZ-10) 124 +/- 18 238 +/- 29 160 +/- 22 267 +/- 25 0.016536 0.0100522 0.011395 0.1261566 0.0668083 ZNF134 zinc finger protein 134 (clone pHZ-15) 473 +/- 12 593 +/- 38 437 +/- 27 544 +/- 37 -0.049071 0.0233991 0.04835 0.0086541 0.3033516 ZNF142 zinc finger protein 142 (clone pHZ-49) 769 +/- 26 723 +/- 22 760 +/- 22 633 +/- 26 -0.068212 -0.217211 -0.005034 0.4714697 0.0379537 ZNF154 zinc finger protein 154 (pHZ-92) 164 +/- 17 242 +/- 25 176 +/- 18 248 +/- 31 0.223358 0.0288284 0.087217 0.000705 0.0149402 ZNF16 zinc finger protein 16 568 +/- 69 420 +/- 40 479 +/- 21 350 +/- 25 -0.089977 -0.099141 -0.004059 0.2275985 0.0013397 ZNF161 zinc finger protein 161 1955 +/- 205 2188 +/- 214 1741 +/- 43 1557 +/- 39 -0.029816 0.4513335 -0.01118 0.120171 0.0720792 ZNF197 zinc finger protein 197 370 +/- 35 200 +/- 10 492 +/- 28 326 +/- 18 1.85E-05 -0.00368 -0.000982 0.0070485 0.01695 ZNF200 zinc finger protein 200 442 +/- 11 377 +/- 17 482 +/- 19 399 +/- 22 0.061584 -0.011261 -0.018821 0.003 0.0151804 ZNF238 zinc finger protein 238 7398 +/- 390 6014 +/- 471 8244 +/- 127 6592 +/- 730 0.049554 -0.047964 -0.08576 0.1522323 0.5300615 ZNF25 zinc finger protein 25 1239 +/- 41 1519 +/- 51 1305 +/- 50 1653 +/- 73 0.004793 0.0018547 0.00525 0.0858856 0.0659915 ZNF278 zinc finger protein 278 239 +/- 11 277 +/- 12 317 +/- 19 300 +/- 16 0.019184 0.0427149 -0.486719 0.0601858 0.7465071 ZNF285 zinc finger protein 285 234 +/- 6 184 +/- 8 245 +/- 9 194 +/- 13 0.258421 -0.000899 -0.014433 0.0031872 0.0029601 ZNF304 zinc finger protein 304 417 +/- 19 402 +/- 10 433 +/- 15 375 +/- 12 -0.668323 -0.484143 -0.014962 0.3962942 0.004852 ZNF313 zinc finger protein 313 694 +/- 43 845 +/- 53 607 +/- 21 631 +/- 16 -0.006952 0.0520928 0.396094 0.033531 0.7041382 ZNF323 zinc finger protein 323 162 +/- 15 187 +/- 22 236 +/- 13 296 +/- 17 0.001103 0.368404 0.023073 0.6848203 0.0231175 ZNF336 zinc finger protein 336 1688 +/- 83 1861 +/- 80 1511 +/- 33 1629 +/- 15 -0.001594 0.1653536 0.013896 0.0909008 0.1093542 ZNF342 zinc finger protein 342 170 +/- 21 134 +/- 18 176 +/- 17 96 +/- 14 -0.574278 -0.232631 -0.00472 0.2812068 0.0030706 ZNF347 zinc finger protein 347 126 +/- 11 178 +/- 7 162 +/- 16 181 +/- 15 0.030671 0.0042757 0.409142 0.0166876 0.8325214 ZNF358 zinc finger protein 358 556 +/- 13 684 +/- 43 872 +/- 81 877 +/- 12 0.000402 0.028655 0.953891 0.0864711 0.9796446 ZNF384 zinc finger protein 384 657 +/- 34 714 +/- 19 682 +/- 19 758 +/- 21 0.164487 0.1755146 0.026243 0.0597001 0.0489691 ZNF416 zinc finger protein 416 101 +/- 8 139 +/- 8 105 +/- 12 116 +/- 13 -0.474243 0.0079955 0.556996 0.0169251 0.5581361 ZNF435 zinc finger protein 435 110 +/- 9 147 +/- 5 143 +/- 12 160 +/- 5 0.033286 0.0094135 0.248189 0.0044561 0.7177566 ZNF436 zinc finger protein 436 845 +/- 77 754 +/- 83 1185 +/- 39 1061 +/- 36 0.000267 -0.443221 -0.045877 0.4482469 0.0157843 ZNF44 zinc finger protein 44 227 +/- 19 193 +/- 5 249 +/- 13 199 +/- 12 0.209191 -0.132476 -0.02205 0.4477143 0.2000242 ZNF45 zinc finger protein 45 279 +/- 18 336 +/- 17 332 +/- 22 400 +/- 18 0.001286 0.0495013 0.045354 0.2410449 0.225943 ZNF452 zinc finger protein 452 148 +/- 21 151 +/- 15 209 +/- 9 177 +/- 7 0.014954 0.8964179 -0.023525 0.9426293 0.0763942 ZNF469 zinc finger protein 469 394 +/- 35 350 +/- 17 320 +/- 12 250 +/- 18 -0.002244 -0.294886 -0.015176 0.2768855 0.1902113 ZNF501 zinc finger protein 501 137 +/- 4 217 +/- 10 206 +/- 8 206 +/- 8 0.044857 0.0002728 -0.95366 0.0016356 0.8863999 ZNF503 zinc finger protein 503 989 +/- 27 822 +/- 31 1260 +/- 34 1165 +/- 60 1.25E-06 -0.002239 -0.214355 0.0017121 0.1866212

S1- alphabetical list of aging-related genes.xls Page 38 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG in CA in DG ZNF527 zinc finger protein 527 174 +/- 9 109 +/- 12 181 +/- 11 142 +/- 18 0.083895 -0.001326 -0.10689 0.0387104 0.463509 ZNF529 zinc finger protein 529 1055 +/- 71 885 +/- 61 1009 +/- 20 833 +/- 33 -0.45572 -0.101266 -0.003095 0.113982 0.0024192 ZNF561 zinc finger protein 561 444 +/- 41 512 +/- 38 329 +/- 24 397 +/- 16 -0.001359 0.2492244 0.046431 0.617956 0.0441045 ZNF564 zinc finger protein 564 324 +/- 32 430 +/- 24 307 +/- 19 390 +/- 12 -0.151689 0.0253231 0.006176 0.0877133 0.046735 ZNF573 zinc finger protein 573 187 +/- 34 144 +/- 13 251 +/- 30 152 +/- 28 0.039697 -0.279536 -0.039265 0.5620131 0.4317854 ZNF585A zinc finger protein 585A 327 +/- 14 417 +/- 21 339 +/- 13 322 +/- 21 -0.117363 0.0053951 -0.492896 0.0066549 0.2596855 ZNF586 zinc finger protein 586 316 +/- 21 232 +/- 26 438 +/- 20 279 +/- 34 0.00204 -0.031863 -0.005803 0.0353043 0.0017489 ZNF592 zinc finger protein 592 288 +/- 9 242 +/- 16 283 +/- 13 268 +/- 16 0.293743 -0.040746 -0.501622 0.141727 0.5779437 ZNF596 zinc finger protein 596 360 +/- 12 434 +/- 25 392 +/- 8 446 +/- 11 0.182693 0.0291735 0.004146 0.0037791 0.0013029 ZNF598 zinc finger protein 598 783 +/- 25 699 +/- 38 826 +/- 24 723 +/- 33 0.41735 -0.098271 -0.035159 0.0232282 0.0712391 ZNF615 zinc finger protein 615 148 +/- 12 112 +/- 12 154 +/- 12 102 +/- 14 -0.762868 -0.057015 -0.019856 0.2040706 0.2784524 ZNF622 zinc finger protein 622 946 +/- 28 1045 +/- 22 741 +/- 18 848 +/- 18 -1.86E-05 0.0219261 0.002302 0.0210693 0.0499573 ZNF624 zinc finger protein 624 203 +/- 12 260 +/- 11 231 +/- 15 261 +/- 14 0.183643 0.0053659 0.177366 0.0006624 0.2310938 ZNF626 Zinc finger protein 626 262 +/- 29 140 +/- 14 248 +/- 23 150 +/- 12 -0.967662 -0.006163 -0.005929 0.0368409 0.041037 ZNF650 zinc finger protein 650 1129 +/- 62 1186 +/- 66 1399 +/- 98 1075 +/- 82 0.299377 0.5365531 -0.031841 0.0619822 0.2010406 ZNF652 Zinc finger protein 652 479 +/- 32 629 +/- 35 465 +/- 24 532 +/- 28 -0.092059 0.0102613 0.106058 0.032785 0.2717889 ZNF658 zinc finger protein 658 201 +/- 19 171 +/- 8 278 +/- 14 206 +/- 22 0.013475 -0.206711 -0.026069 0.2586914 0.1252363 ZNF664 zinc finger protein 664 4855 +/- 461 4156 +/- 264 5217 +/- 185 4351 +/- 285 0.219105 -0.224378 -0.037811 0.4622582 0.189499 ZNF669 zinc finger protein 669 417 +/- 25 647 +/- 62 504 +/- 23 634 +/- 44 0.333179 0.011644 0.038529 0.017253 0.0599344 ZNF697 zinc finger protein 697 458 +/- 22 383 +/- 9 394 +/- 27 303 +/- 1 -0.000306 -0.018619 -0.020789 0.0263098 0.033517 ZNF70 Zinc finger protein 70 162 +/- 13 219 +/- 18 207 +/- 13 233 +/- 8 0.087186 0.0282527 0.14106 0.0447341 0.5020141 ZNF74 zinc finger protein 74 147 +/- 15 91 +/- 13 170 +/- 12 113 +/- 14 0.06611 -0.018936 -0.01637 0.1127438 0.2064913 ZNF75 zinc finger protein 75 (D8C6) 118 +/- 9 171 +/- 11 232 +/- 23 191 +/- 11 0.013173 0.0039383 -0.160314 0.0464456 0.0901358 ZNF83 zinc finger protein 83 385 +/- 25 545 +/- 35 388 +/- 21 477 +/- 31 -0.212678 0.0046795 0.046906 0.0006789 0.0442847 ZNF9 zinc finger protein 9 6431 +/- 219 5316 +/- 196 5728 +/- 139 4867 +/- 76 -0.000164 -0.00362 -0.000742 0.0312627 0.0114342 ZNFN1A1 zinc finger protein, subfamily 1A, 1 (Ikaros) 114 +/- 15 175 +/- 22 102 +/- 11 150 +/- 6 -0.194663 0.0449452 0.005623 0.0083714 0.0808889 ZNFN1A5 zinc finger protein, subfamily 1A, 5 579 +/- 151 1239 +/- 161 624 +/- 101 993 +/- 136 -0.280489 0.0136385 0.061689 0.0075454 0.0129977 ZNHIT3 zinc finger, HIT type 3 1626 +/- 109 1266 +/- 38 1613 +/- 77 1374 +/- 14 0.522316 -0.01978 -0.026213 0.0269002 0.0389438 ZNRD1 zinc ribbon domain containing 1 926 +/- 41 625 +/- 37 853 +/- 26 720 +/- 30 0.940327 -0.000269 -0.009908 0.0596257 0.0992138 ZNRF1 zinc and ring finger 1 184 +/- 15 139 +/- 13 202 +/- 22 172 +/- 11 0.259778 -0.045444 -0.273991 0.0873131 0.2765162 ZNRF2 zinc and ring finger 2 122 +/- 11 116 +/- 9 87 +/- 7 125 +/- 7 -0.157226 -0.667415 0.004702 0.7264889 0.0789072 ZRANB1 zinc finger, RAN-binding domain containing 1 849 +/- 44 1040 +/- 45 887 +/- 57 1073 +/- 52 0.411994 0.012515 0.039302 0.2520777 0.2650146 ZRANB3 zinc finger, RAN-binding domain containing 3 569 +/- 41 434 +/- 28 513 +/- 24 455 +/- 29 -0.646283 -0.024725 -0.165971 0.1341786 0.5240794 ZWINT ZW10 interactor 592 +/- 49 356 +/- 55 462 +/- 44 332 +/- 21 -0.105522 -0.009325 -0.032176 0.0074061 0.0888791 ZXDB zinc finger, X-linked, duplicated B 667 +/- 61 496 +/- 39 665 +/- 46 479 +/- 61 -0.95921 -0.044559 -0.041047 0.2601647 0.2486741 ZYG11B Zyg-11 homolog B (C. elegans) 783 +/- 95 1096 +/- 114 916 +/- 54 1329 +/- 49 0.004684 0.0627527 0.000304 0.2823152 0.0091151 ZZEF1 zinc finger, ZZ-type with EF-hand domain 1 1717 +/- 133 1604 +/- 63 1605 +/- 32 1429 +/- 41 -0.124003 -0.465891 -0.009886 0.6526544 0.0785597

S1- alphabetical list of aging-related genes.xls Page 39 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI Overexpressed in CA 2'-PDE 2'-phosphodiesterase 274 +/- 14 302 +/- 11 344 +/- 8 359 +/- 8 5.62E-05 0.1501669 0.214248 0.1382908 0.676623 AADACL1 arylacetamide deacetylase-like 1 2372 +/- 68 2331 +/- 133 2570 +/- 66 2679 +/- 108 0.001544 -0.787768 0.419757 0.1454127 0.9179538 AASDHPPTaminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase 8050 +/- 338 7445 +/- 313 8380 +/- 331 7797 +/- 378 0.043071 -0.217551 -0.277423 0.6774587 0.9614512 ABCA5 ATP-binding cassette, sub-family A (ABC1), member 5 539 +/- 47 572 +/- 45 663 +/- 71 669 +/- 19 0.028807 0.6232489 0.938339 0.8611567 0.9380525 ABCB9 ATP-binding cassette, sub-family B (MDR/TAP), member 9 81 +/- 12 75 +/- 8 142 +/- 25 137 +/- 26 0.011372 -0.714643 -0.89493 0.6969868 0.4761067 ABCG4 ATP-binding cassette, sub-family G (WHITE), member 4 113 +/- 5 98 +/- 12 166 +/- 10 159 +/- 19 2.64E-05 -0.301902 -0.76083 0.0767186 0.462626 ABHD13 abhydrolase domain containing 13 130 +/- 12 111 +/- 9 150 +/- 11 146 +/- 10 0.008996 -0.247523 -0.793308 0.5320683 0.9735457 ABHD14A abhydrolase domain containing 14A 1760 +/- 56 1515 +/- 91 1973 +/- 103 1818 +/- 29 0.010547 -0.048824 -0.200935 0.0371071 0.3262792 ABI1 abl-interactor 1 513 +/- 68 396 +/- 59 675 +/- 49 584 +/- 41 0.001619 -0.220504 -0.19246 0.3330538 0.161837 ABI2 Abl interactor 2 4246 +/- 313 3508 +/- 132 4833 +/- 237 4953 +/- 337 0.002763 -0.06816 0.778145 0.0315127 0.8189551 ABP1 Amiloride binding protein 1 (amine oxidase (copper-containing)) 105 +/- 10 165 +/- 23 154 +/- 17 181 +/- 18 0.021482 0.0527017 0.302632 5.18E-05 0.0770133 ACADSB acyl-Coenzyme A dehydrogenase, short/branched chain 1081 +/- 68 908 +/- 83 1155 +/- 57 1004 +/- 111 0.04789 -0.140293 -0.270539 0.1869661 0.2566964 ACBD6 acyl-Coenzyme A binding domain containing 6 209 +/- 23 207 +/- 7 290 +/- 25 261 +/- 19 0.001028 -0.936329 -0.377156 0.902757 0.5933015 ACD adrenocortical dysplasia homolog (mouse) 445 +/- 5 448 +/- 27 510 +/- 18 519 +/- 24 0.039645 0.9180367 0.77176 0.5878682 0.3935524 ACHE acetylcholinesterase (Yt blood group) 247 +/- 28 246 +/- 21 390 +/- 22 370 +/- 26 0.000271 -0.966857 -0.571979 0.6940565 0.4052951 ACLY ATP citrate lyase 2336 +/- 139 2016 +/- 72 2429 +/- 110 2221 +/- 46 0.042315 -0.07669 -0.127295 0.1508285 0.192972 ACTC actin, alpha, cardiac muscle 84 +/- 15 206 +/- 35 98 +/- 11 212 +/- 35 0.021907 0.0155645 0.029736 0.0980108 0.1657953 ACTL6B actin-like 6B 789 +/- 27 810 +/- 60 981 +/- 47 987 +/- 32 0.002024 0.7508105 0.916057 0.5004999 0.4981511 ACTN1 actinin, alpha 1 3839 +/- 279 3397 +/- 240 4211 +/- 216 4122 +/- 276 0.032517 -0.258876 -0.804727 0.2014078 0.8719828 ACTR10 actin-related protein 10 homolog (S. cerevisiae) 6172 +/- 108 6822 +/- 248 6301 +/- 210 7510 +/- 229 0.046595 0.0483003 0.003988 0.5901922 0.0845271 ACVR1C activin A receptor, type IC 504 +/- 117 394 +/- 115 1153 +/- 118 1015 +/- 47 2.67E-05 -0.517474 -0.312178 0.419665 0.2356285 ACY1L2 aminoacylase 1-like 2 224 +/- 39 230 +/- 24 450 +/- 41 321 +/- 40 0.001236 0.8951819 -0.050237 0.5937618 0.3209364 ADAM23 ADAM metallopeptidase domain 23 502 +/- 63 450 +/- 44 637 +/- 76 595 +/- 48 0.015084 -0.514329 -0.651493 0.243668 0.4382308 ADAMTS8 ADAM metallopeptidase with thrombospondin type 1 motif, 8 301 +/- 27 381 +/- 34 411 +/- 34 447 +/- 27 0.033527 0.0930654 0.436453 0.2897654 0.3255868 ADCYAP1Radenylate cyclase activating polypeptide 1 (pituitary) receptor type I 142 +/- 22 159 +/- 16 263 +/- 11 230 +/- 39 0.001271 0.5497295 -0.455398 0.3994654 0.5230391 ADD3 adducin 3 (gamma) 2311 +/- 330 3058 +/- 364 3069 +/- 265 3733 +/- 91 0.020001 0.1598374 0.054267 0.1569602 0.2721863 ADIPOQ adiponectin, C1Q and collagen domain containing 232 +/- 23 224 +/- 30 348 +/- 34 306 +/- 18 0.011454 -0.83889 -0.30877 0.6419291 0.2673007 ADIPOR1 adiponectin receptor 1 1312 +/- 71 1217 +/- 68 1418 +/- 80 1497 +/- 61 0.021222 -0.359681 0.452814 0.7762157 0.2931045 ADRA1D adrenergic, alpha-1D-, receptor 375 +/- 88 305 +/- 60 626 +/- 89 648 +/- 71 0.0008 -0.527737 0.848975 0.4919625 0.9511084 AFF3 AF4/FMR2 family, member 3 128 +/- 17 237 +/- 14 224 +/- 20 358 +/- 44 0.000454 0.0007359 0.034613 0.0013776 0.0003071 AFTIPHILINaftiphilin protein 689 +/- 9 552 +/- 54 739 +/- 50 699 +/- 28 0.034714 -0.050685 -0.502967 0.1353892 0.6185892 AGPAT1 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, al 3385 +/- 154 3061 +/- 119 3681 +/- 81 3655 +/- 174 0.004437 -0.128538 -0.897533 0.0854121 0.3155426 AGTRL1 angiotensin II receptor-like 1 747 +/- 232 1295 +/- 454 1735 +/- 367 2547 +/- 206 0.002059 0.3161342 0.091231 0.7427029 0.2258739 AHDC1 AT hook, DNA binding motif, containing 1 339 +/- 26 276 +/- 12 411 +/- 27 349 +/- 35 0.023351 -0.062351 -0.206902 0.0844899 0.430451 AK2 adenylate kinase 2 232 +/- 19 205 +/- 13 283 +/- 15 268 +/- 21 0.001205 -0.258729 -0.573395 0.6323663 0.7294777 AK5 adenylate kinase 5 1192 +/- 282 777 +/- 131 1479 +/- 76 1553 +/- 208 0.018182 -0.224209 0.751364 0.2526122 0.4765015 AKAP1 A kinase (PRKA) anchor protein 1 1834 +/- 110 1934 +/- 117 2107 +/- 94 2316 +/- 204 0.008273 0.5466616 0.389688 0.4308971 0.0718354 AKAP8 A kinase (PRKA) anchor protein 8 442 +/- 54 500 +/- 46 573 +/- 28 668 +/- 58 0.00162 0.4311339 0.190676 0.2053949 0.027958 AKR1CL2 aldo-keto reductase family 1, member C-like 2 153 +/- 15 135 +/- 24 186 +/- 10 168 +/- 19 0.006243 -0.538217 -0.450109 0.3480839 0.1868468 AKT1S1 AKT1 substrate 1 (proline-rich) 1591 +/- 147 1450 +/- 58 1862 +/- 67 1671 +/- 81 0.005553 -0.402899 -0.105151 0.4278656 0.2906017 ALDH3A2 aldehyde dehydrogenase 3 family, member A2 891 +/- 72 1013 +/- 70 1099 +/- 68 1247 +/- 102 0.014821 0.2502131 0.26533 0.3635668 0.7900991 ALDH5A1 aldehyde dehydrogenase 5 family, member A1 (succinate-semialdehyde dehydrogenase) 2044 +/- 186 2461 +/- 205 2314 +/- 135 2541 +/- 155 0.026427 0.163796 0.299879 0.3742309 0.4976003 ALPL alkaline phosphatase, liver/bone/kidney 99 +/- 9 108 +/- 9 142 +/- 11 116 +/- 11 0.029567 0.4960101 -0.13156 0.865689 0.5622892 ANAPC10 anaphase promoting complex subunit 10 140 +/- 9 189 +/- 9 212 +/- 8 217 +/- 21 0.001658 0.003833 0.804792 0.0042811 0.1671211

S2-genes differentially expressed across region.xls Page 1 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ANKFY1 ankyrin repeat and FYVE domain containing 1 411 +/- 39 333 +/- 24 438 +/- 14 444 +/- 18 0.021417 -0.126179 0.816911 0.1019464 0.3766156 ANKMY2 ankyrin repeat and MYND domain containing 2 3980 +/- 219 3697 +/- 250 4113 +/- 165 4265 +/- 127 0.033575 -0.413618 0.48654 0.2056545 0.6121396 ANKRD11 ankyrin repeat domain 11 147 +/- 24 240 +/- 28 262 +/- 22 250 +/- 72 0.044845 0.0307121 -0.879094 0.0139103 0.3395989 ANKRD13Bankyrin repeat domain 13B 672 +/- 70 607 +/- 28 807 +/- 53 826 +/- 80 0.019989 -0.414871 0.850495 0.4833244 0.1845796 ANKRD23 ankyrin repeat domain 23 382 +/- 37 460 +/- 36 555 +/- 43 493 +/- 41 0.017891 0.1611868 -0.322199 0.0418279 0.7286784 ANKRD29 ankyrin repeat domain 29 698 +/- 63 868 +/- 91 1108 +/- 49 1287 +/- 104 6.44E-06 0.1609981 0.173041 0.2651558 0.3558069 ANKRD34 ankyrin repeat domain 34 1681 +/- 72 1443 +/- 63 1781 +/- 88 1714 +/- 34 0.039068 -0.031915 -0.501221 0.0025436 0.8755633 ANKRD43 ankyrin repeat domain 43 1626 +/- 115 1329 +/- 135 1930 +/- 112 1590 +/- 92 0.041881 -0.125069 -0.044003 0.1207345 0.1464073 ANXA11 annexin A11 2586 +/- 271 2346 +/- 337 4026 +/- 210 4033 +/- 163 1.96E-05 -0.592439 0.980271 0.4934545 0.6586726 AOF1 amine oxidase (flavin containing) domain 1 169 +/- 18 130 +/- 10 243 +/- 18 242 +/- 16 0.000487 -0.096114 -0.979838 0.095309 0.4089211 AP1G1 adaptor-related protein complex 1, gamma 1 subunit 998 +/- 57 879 +/- 39 1020 +/- 43 1094 +/- 57 0.037127 -0.12403 0.3245 0.1385012 0.3912204 AP1G2 adaptor-related protein complex 1, gamma 2 subunit 408 +/- 27 425 +/- 41 631 +/- 65 604 +/- 42 0.000529 0.7400845 -0.734539 0.7774056 0.9823444 AP2A1 adaptor-related protein complex 2, alpha 1 subunit 783 +/- 97 744 +/- 70 1165 +/- 64 1129 +/- 66 0.000548 -0.74769 -0.704203 0.5428389 0.6002089 AP2A2 adaptor-related protein complex 2, alpha 2 subunit 2448 +/- 81 2854 +/- 170 2751 +/- 48 3195 +/- 203 0.001975 0.0671658 0.093842 0.4204682 0.3764182 AP3B2 adaptor-related protein complex 3, beta 2 subunit 2564 +/- 199 2544 +/- 202 2734 +/- 85 3030 +/- 117 0.025402 -0.944737 0.07538 0.4439021 0.2170597 AP4S1 adaptor-related protein complex 4, sigma 1 subunit 336 +/- 50 195 +/- 7 440 +/- 40 279 +/- 34 0.006951 -0.035594 -0.012958 0.0223472 0.0475868 APBA2BP amyloid beta (A4) precursor protein-binding, family A, member 2 binding protein 249 +/- 22 279 +/- 31 447 +/- 30 429 +/- 26 0.000693 0.4430347 -0.659582 0.9747118 0.5733366 APCDD1 adenomatosis polyposis coli down-regulated 1 2086 +/- 164 2327 +/- 131 2605 +/- 98 2804 +/- 143 0.000106 0.2778884 0.287828 0.3922611 0.4336873 APH1A anterior pharynx defective 1 homolog A (C. elegans) 223 +/- 32 209 +/- 27 309 +/- 20 337 +/- 59 0.013161 -0.74851 0.669645 0.5503959 0.8284411 API5 apoptosis inhibitor 5 1659 +/- 24 1825 +/- 54 1812 +/- 42 1906 +/- 76 0.037835 0.0265735 0.322472 0.0189811 0.0551939 APIP APAF1 interacting protein 342 +/- 22 396 +/- 23 428 +/- 26 427 +/- 27 0.039992 0.1232617 -0.987226 0.0717332 0.9139034 APLP2 Amyloid beta (A4) precursor-like protein 2 397 +/- 24 319 +/- 21 448 +/- 28 403 +/- 22 0.010772 -0.037329 -0.240439 0.077951 0.5159072 APRIN androgen-induced proliferation inhibitor 2075 +/- 34 1723 +/- 57 2125 +/- 61 1961 +/- 40 0.027176 -0.000695 -0.054459 7.079E-06 0.033862 ARF5 ADP-ribosylation factor 5 2506 +/- 85 2334 +/- 218 2867 +/- 103 3044 +/- 177 0.000129 -0.487833 0.418757 0.0661736 0.6368668 ARHGAP28Rho GTPase activating protein 28 120 +/- 6 123 +/- 16 146 +/- 9 142 +/- 13 0.004923 0.844647 -0.82487 0.7439749 0.6618588 ARHGAP5 Rho GTPase activating protein 5 2457 +/- 65 2709 +/- 129 2871 +/- 96 3130 +/- 126 0.000148 0.1239275 0.140657 0.3775916 0.7391119 ARHGDIA Rho GDP dissociation inhibitor (GDI) alpha 1206 +/- 41 1174 +/- 74 1423 +/- 42 1527 +/- 50 0.000727 -0.712096 0.145065 0.2967029 0.3901063 ARHGEF11Rho guanine nucleotide exchange factor (GEF) 11 658 +/- 70 564 +/- 45 969 +/- 43 769 +/- 57 0.000579 -0.290212 -0.023698 0.2544702 0.0498946 ARHGEF4 Rho guanine nucleotide exchange factor (GEF) 4 357 +/- 44 396 +/- 44 544 +/- 38 584 +/- 28 0.00026 0.5440807 0.415946 0.6376248 0.4753666 ARHGEF9 Cdc42 guanine nucleotide exchange factor (GEF) 9 7240 +/- 569 6125 +/- 451 8090 +/- 208 7683 +/- 152 0.005476 -0.157349 -0.148485 0.1078374 0.2790658 ARID1A AT rich interactive domain 1A (SWI- like) 2884 +/- 31 3123 +/- 159 3720 +/- 77 3607 +/- 106 1.91E-05 0.1960555 -0.410718 0.1496971 0.9241797 ARID1B AT rich interactive domain 1B (SWI1-like) 517 +/- 50 742 +/- 58 815 +/- 107 980 +/- 67 0.000873 0.0153366 0.224649 0.1181921 0.401326 ARL16 ADP-ribosylation factor-like 16 257 +/- 44 228 +/- 17 301 +/- 34 294 +/- 29 0.026097 -0.563512 -0.872004 0.426811 0.8893289 ARL2BP ADP-ribosylation factor-like 2 binding protein 993 +/- 81 1080 +/- 91 1206 +/- 39 1294 +/- 58 0.004021 0.4925035 0.250814 0.3229259 0.0634048 ARL3 ADP-ribosylation factor-like 3 1333 +/- 68 1158 +/- 83 1687 +/- 85 1504 +/- 42 0.00067 -0.13414 -0.092918 0.050519 0.2444836 ARL5A ADP-ribosylation factor-like 5A 486 +/- 29 436 +/- 13 580 +/- 35 497 +/- 28 0.000409 -0.161788 -0.094801 0.0572492 0.0387886 ARL6 ADP-ribosylation factor-like 6 281 +/- 26 222 +/- 15 332 +/- 29 308 +/- 39 0.031421 -0.086062 -0.640371 0.2411581 0.7018738 ARL8A ADP-ribosylation factor-like 8A 508 +/- 44 550 +/- 48 703 +/- 29 609 +/- 35 0.002964 0.5347678 -0.073656 0.4696596 0.0959133 ARMC5 armadillo repeat containing 5 470 +/- 32 534 +/- 18 557 +/- 18 573 +/- 20 0.007836 0.1212344 0.55613 0.0882489 0.4560272 ARNTL2 aryl hydrocarbon receptor nuclear translocator-like 2 97 +/- 9 122 +/- 10 161 +/- 21 211 +/- 17 0.000537 0.0902796 0.100973 0.2192966 0.0874126 ARPC4 actin related protein 2/3 complex, subunit 4, 20kDa 426 +/- 90 601 +/- 106 668 +/- 134 761 +/- 120 0.004356 0.2392811 0.616102 0.09266 0.2413576 ARPP-21 cyclic AMP-regulated phosphoprotein, 21 kD 224 +/- 24 296 +/- 23 275 +/- 24 340 +/- 21 0.029844 0.0558941 0.071046 0.1586405 0.0442336 ARS2 ARS2 protein 131 +/- 21 120 +/- 22 196 +/- 25 179 +/- 18 0.047929 -0.730739 -0.588868 0.8098916 0.6827881 AS3MT arsenic (+3 oxidation state) methyltransferase 275 +/- 16 260 +/- 13 335 +/- 16 321 +/- 5 0.000133 -0.466809 -0.404534 0.6779068 0.4678598 ASB6 ankyrin repeat and SOCS box-containing 6 176 +/- 8 150 +/- 8 238 +/- 19 208 +/- 4 6.79E-05 -0.052557 -0.185877 0.1854066 0.3284327

S2-genes differentially expressed across region.xls Page 2 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ASPH aspartate beta-hydroxylase 294 +/- 56 432 +/- 58 456 +/- 44 635 +/- 89 0.000322 0.1194833 0.122998 0.3048759 0.3573241 ASPHD2 aspartate beta-hydroxylase domain containing 2 919 +/- 80 730 +/- 79 1141 +/- 46 992 +/- 32 0.002157 -0.122917 -0.027085 0.0684548 0.0557951 ASTN2 Astrotactin 2 149 +/- 10 185 +/- 27 178 +/- 20 260 +/- 21 0.037549 0.2540618 0.021935 0.4993867 0.2857922 ATF2 activating transcription factor 2 294 +/- 27 312 +/- 27 407 +/- 29 463 +/- 35 0.001418 0.6380896 0.254474 0.8619435 0.9481229 ATG4B ATG4 autophagy related 4 homolog B (S. cerevisiae) 489 +/- 25 632 +/- 27 517 +/- 23 734 +/- 11 0.023955 0.0030799 5.64E-05 0.0439593 0.0065325 ATP1B1 ATPase, Na+/K+ transporting, beta 1 polypeptide 16024 +/- 756 14419 +/- 558 16669 +/- 305 16773 +/- 630 0.011541 -0.121283 0.886514 0.0637325 0.4483327 ATP1B2 ATPase, Na+/K+ transporting, beta 2 polypeptide 2211 +/- 353 1622 +/- 108 3541 +/- 507 2379 +/- 126 1.21E-05 -0.162072 -0.070906 0.3327971 0.2195488 ATP5D ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit 1719 +/- 90 1425 +/- 89 2012 +/- 103 1836 +/- 84 0.000518 -0.042683 -0.215555 0.1947563 0.6087121 ATP5J ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 1190 +/- 81 1279 +/- 83 1365 +/- 104 1473 +/- 52 0.020777 0.4571621 0.380695 0.7932993 0.4209813 ATP6V0A1 ATPase, H+ transporting, lysosomal V0 subunit a1 1437 +/- 216 1172 +/- 163 2027 +/- 135 2029 +/- 122 0.001694 -0.351797 0.991517 0.3783119 0.9022873 ATP6V0C Homo sapiens, clone IMAGE:5500209, mRNA 190 +/- 15 205 +/- 25 273 +/- 24 251 +/- 30 0.021063 0.6160935 -0.583558 0.783347 0.540117 ATP6V0E2 ATPase, H+ transporting V0 subunit E2-like (rat) 8864 +/- 439 7917 +/- 479 10765 +/- 390 11364 +/- 442 3.58E-05 -0.17568 0.336823 0.102467 0.5368466 ATP6V1A ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A 6850 +/- 355 6520 +/- 371 7663 +/- 310 7712 +/- 397 6.03E-05 -0.535105 0.923551 0.1879775 0.5609475 ATP8B2 ATPase, Class I, type 8B, member 2 1260 +/- 36 1102 +/- 43 1508 +/- 74 1279 +/- 39 0.000662 -0.018718 -0.027391 0.0716008 0.1292793 ATPIF1 ATPase inhibitory factor 1 1235 +/- 44 1173 +/- 19 1343 +/- 67 1274 +/- 57 0.035568 -0.230525 -0.456891 0.3872839 0.8454393 ATXN7L1 ataxin 7-like 1 462 +/- 25 555 +/- 22 612 +/- 31 626 +/- 34 0.003912 0.0190188 0.771269 0.0840158 0.431721 AUH AU RNA binding protein/enoyl-Coenzyme A hydratase 1067 +/- 86 1010 +/- 43 1076 +/- 20 1120 +/- 56 0.001395 -0.572988 0.489241 0.5370059 0.9428233 AUP1 ancient ubiquitous protein 1 1882 +/- 51 1935 +/- 42 1952 +/- 46 2088 +/- 56 0.002488 0.4357029 0.096496 0.2888487 0.0663254 B3GALNT1UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 (Globoside b 1088 +/- 64 964 +/- 77 1325 +/- 116 1134 +/- 81 0.024793 -0.243063 -0.210777 0.1477903 0.0797626 B3GNT4 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4 355 +/- 36 310 +/- 36 516 +/- 29 517 +/- 17 9.93E-06 -0.400114 0.95899 0.2660171 0.6913465 B3GNTL1 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 215 +/- 11 174 +/- 8 238 +/- 21 202 +/- 7 0.016043 -0.011299 -0.156849 0.0343221 0.3937487 B4GALNT1beta-1,4-N-acetyl-galactosaminyl transferase 1 823 +/- 133 661 +/- 89 961 +/- 50 984 +/- 51 0.014772 -0.339789 0.749304 0.2584943 0.816825 B4GALNT4beta-1,4-N-acetyl-galactosaminyl transferase 4 304 +/- 26 292 +/- 36 553 +/- 22 559 +/- 48 1.53E-06 -0.797378 0.913627 0.2124745 0.5069836 B4GALT6 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6 3702 +/- 250 3117 +/- 295 4186 +/- 123 4025 +/- 189 0.002035 -0.16124 -0.498299 0.1132214 0.2258758 BAG1 BCL2-associated athanogene 642 +/- 54 817 +/- 54 829 +/- 57 905 +/- 31 0.029825 0.0459185 0.271393 0.2549285 0.394043 BAI1 brain-specific angiogenesis inhibitor 1 410 +/- 32 348 +/- 39 566 +/- 28 470 +/- 36 0.000411 -0.249366 -0.071541 0.0436578 0.0544465 BAIAP2L2 BAI1-associated protein 2-like 2 398 +/- 24 459 +/- 32 514 +/- 48 575 +/- 36 0.000532 0.1596491 0.330204 0.2301458 0.2857937 BASP1 brain abundant, membrane attached signal protein 1 21373 +/- 964 19251 +/- 1027 23568 +/- 675 24329 +/- 669 0.000747 -0.162978 0.444189 0.0563427 0.9034569 BAT3 HLA-B associated transcript 3 2559 +/- 103 2642 +/- 81 2825 +/- 91 2890 +/- 81 0.016473 0.5371019 0.607559 0.6693524 0.8267535 BBS1 Bardet-Biedl syndrome 1 113 +/- 24 85 +/- 11 152 +/- 16 143 +/- 12 0.013695 -0.341177 -0.678375 0.2367785 0.3067299 BCAT1 branched chain aminotransferase 1, cytosolic 167 +/- 14 163 +/- 20 234 +/- 24 247 +/- 8 0.003387 -0.859226 0.649539 0.7129766 0.6276384 BCL10 B-cell CLL/lymphoma 10 367 +/- 32 332 +/- 22 459 +/- 20 380 +/- 15 0.010289 -0.395955 -0.011414 0.3654709 0.0460348 BCL7A B-cell CLL/lymphoma 7A 1042 +/- 53 931 +/- 51 1372 +/- 79 1245 +/- 71 0.000271 -0.160016 -0.262591 0.0222234 0.5541953 BCL9L B-cell CLL/lymphoma 9-like 590 +/- 55 534 +/- 38 851 +/- 35 651 +/- 50 0.002662 -0.417435 -0.012774 0.3931402 0.0874792 BCORL1 BCL6 co-repressor-like 1 389 +/- 27 443 +/- 24 433 +/- 27 482 +/- 12 0.005365 0.165648 0.148061 0.2334638 0.1158968 BEX1 brain expressed, X-linked 1 15228 +/- 640 14475 +/- 323 16692 +/- 1062 15384 +/- 600 0.01875 -0.326958 -0.316014 0.3896235 0.5326835 BEXL1 brain expressed X-linked-like 1 12212 +/- 540 11531 +/- 649 13679 +/- 351 14275 +/- 318 0.000607 -0.439546 0.24007 0.0828828 0.8279986 BHLHB3 basic helix-loop-helix domain containing, class B, 3 119 +/- 21 160 +/- 29 214 +/- 15 265 +/- 31 0.000743 0.2717205 0.183697 0.1479952 0.3265801 BHLHB5 basic helix-loop-helix domain containing, class B, 5 117 +/- 38 78 +/- 27 238 +/- 21 192 +/- 35 0.000537 -0.434633 -0.29279 0.5262515 0.5144178 BHLHB8 Basic helix-loop-helix domain containing, class B, 8 143 +/- 16 125 +/- 35 174 +/- 12 164 +/- 19 0.043961 -0.658563 -0.667069 0.4724671 0.2276161 BHLHB9 basic helix-loop-helix domain containing, class B, 9 1246 +/- 54 1079 +/- 55 1348 +/- 40 1378 +/- 74 0.00898 -0.054366 0.738951 0.0366155 0.1918358 BICC1 Bicaudal C homolog 1 (Drosophila) 484 +/- 64 390 +/- 42 763 +/- 49 466 +/- 75 0.010564 -0.249579 -0.012458 0.4693602 0.1136697 BID BH3 interacting domain death agonist 393 +/- 40 414 +/- 40 567 +/- 35 557 +/- 15 5.36E-05 0.7185824 -0.807205 0.9766086 0.4356621 BIRC6 baculoviral IAP repeat-containing 6 (apollon) 348 +/- 15 458 +/- 21 431 +/- 7 524 +/- 30 4.21E-05 0.0020667 0.034166 0.000872 0.0185995 BLOC1S1 biogenesis of lysosome-related organelles complex-1, subunit 1 2581 +/- 187 2677 +/- 93 3051 +/- 286 2823 +/- 198 0.028279 0.6585796 -0.529075 0.6067355 0.8023716

S2-genes differentially expressed across region.xls Page 3 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI BNIP3 BCL2/adenovirus E1B 19kDa interacting protein 3 2942 +/- 368 2074 +/- 55 3533 +/- 262 3525 +/- 283 0.009231 -0.065053 -0.984991 0.1002062 0.7394053 BOP1 block of proliferation 1 292 +/- 18 210 +/- 26 357 +/- 15 258 +/- 9 0.005846 -0.026404 -0.000564 0.0936834 0.0022434 BPY2IP1 BPY2 interacting protein 1 1258 +/- 80 1088 +/- 56 1546 +/- 83 1454 +/- 64 0.000386 -0.115916 -0.403279 0.1065485 0.9397104 BRCC3 BRCA1/BRCA2-containing complex, subunit 3 122 +/- 3 144 +/- 12 177 +/- 11 201 +/- 28 0.002541 0.138862 0.462484 0.0068226 0.2287188 BRD2 bromodomain containing 2 683 +/- 52 469 +/- 53 945 +/- 41 572 +/- 72 0.004681 -0.016525 -0.003474 0.2800474 0.0970544 BRD4 bromodomain containing 4 1364 +/- 60 1532 +/- 76 1613 +/- 77 1817 +/- 168 0.002429 0.113258 0.314125 0.0282218 0.0337512 BRP44 brain protein 44 2917 +/- 183 3180 +/- 167 3323 +/- 135 3325 +/- 141 0.001797 0.313945 0.992651 0.3514422 0.9447145 BRSK1 BR serine/threonine kinase 1 5242 +/- 394 5795 +/- 357 6191 +/- 224 6220 +/- 270 0.022998 0.3228068 0.936685 0.5132612 0.8895316 BRUNOL4 bruno-like 4, RNA binding protein (Drosophila) 529 +/- 80 495 +/- 78 707 +/- 49 713 +/- 69 0.012868 -0.770154 0.947456 0.7203313 0.9861663 BRUNOL5 bruno-like 5, RNA binding protein (Drosophila) 5949 +/- 259 5064 +/- 363 6861 +/- 189 6748 +/- 300 0.003164 -0.078252 -0.760875 0.0320415 0.8382862 BTBD9 BTB (POZ) domain containing 9 986 +/- 112 775 +/- 85 1434 +/- 60 1243 +/- 125 0.000221 -0.167701 -0.21918 0.3738754 0.4306061 BTG1 B-cell translocation gene 1, anti-proliferative 613 +/- 93 775 +/- 199 1509 +/- 170 1482 +/- 63 0.000824 0.4863909 -0.888423 0.7197071 0.845833 BTRC beta-transducin repeat containing 373 +/- 15 369 +/- 16 437 +/- 36 454 +/- 23 0.008498 -0.85504 0.70077 0.7733985 0.4187493 C1QTNF4 C1q and tumor necrosis factor related protein 4 1896 +/- 138 1558 +/- 136 2524 +/- 162 2239 +/- 97 0.000368 -0.112224 -0.168387 0.1473202 0.4586117 C4A complement component 4A (Rodgers blood group) 692 +/- 140 453 +/- 51 820 +/- 173 604 +/- 82 0.035302 -0.157983 -0.297159 0.2052612 0.4115422 CA10 carbonic anhydrase X 1854 +/- 361 1852 +/- 301 4062 +/- 174 3455 +/- 172 3.99E-05 -0.996745 -0.035214 0.9563537 0.1060229 CA4 carbonic anhydrase IV 614 +/- 77 577 +/- 69 979 +/- 67 927 +/- 74 0.000362 -0.727668 -0.616974 0.5360975 0.2270823 CACNA1A calcium channel, voltage-dependent, P/Q type, alpha 1A subunit 961 +/- 89 664 +/- 44 1617 +/- 110 993 +/- 119 0.000194 -0.018645 -0.004193 0.0747641 0.1216223 CACNA1B calcium channel, voltage-dependent, L type, alpha 1B subunit 1982 +/- 101 1808 +/- 172 2242 +/- 49 2084 +/- 149 0.029899 -0.407625 -0.362213 0.0742244 0.5244628 CACNA1C Calcium channel, voltage-dependent, L type, alpha 1C subunit 230 +/- 30 267 +/- 24 443 +/- 36 377 +/- 27 0.001409 0.3637032 -0.174917 0.5685678 0.2007988 CACNA1D calcium channel, voltage-dependent, L type, alpha 1D subunit 275 +/- 14 241 +/- 31 393 +/- 22 346 +/- 16 0.000482 -0.345133 -0.120709 0.2080854 0.3728838 CACNB1 calcium channel, voltage-dependent, beta 1 subunit 1084 +/- 79 825 +/- 44 1214 +/- 70 1044 +/- 36 0.009523 -0.021979 -0.065895 0.0056008 0.145536 CACNB2 calcium channel, voltage-dependent, beta 2 subunit 715 +/- 48 653 +/- 54 799 +/- 59 786 +/- 48 0.029995 -0.416033 -0.86656 0.1161396 0.2420466 CACNG2 calcium channel, voltage-dependent, gamma subunit 2 437 +/- 87 573 +/- 89 862 +/- 54 1025 +/- 88 3.41E-05 0.3000925 0.15818 0.3928038 0.5968357 CADPS Ca2+-dependent secretion activator 840 +/- 160 689 +/- 115 1049 +/- 81 1151 +/- 113 0.002395 -0.461221 0.484948 0.2912296 0.5434116 CALCOCO calcium binding and coiled-coil domain 2 135 +/- 14 182 +/- 16 231 +/- 15 267 +/- 26 0.000718 0.0542604 0.274321 0.0064576 0.2571646 CALD1 caldesmon 1 84 +/- 6 130 +/- 15 132 +/- 8 136 +/- 6 0.005626 0.0251705 0.710226 0.0022 0.752043 CALM1 calmodulin 1 (phosphorylase kinase, delta) 19009 +/- 992 17370 +/- 903 20650 +/- 696 20727 +/- 744 0.007508 -0.249949 0.940973 0.1815429 0.9189091 CALM3 calmodulin 3 (phosphorylase kinase, delta) 16267 +/- 1508 13584 +/- 984 17973 +/- 885 18301 +/- 521 0.003687 -0.172039 0.757292 0.0933515 0.8406487 CALU calumenin 1229 +/- 91 1066 +/- 40 1331 +/- 57 1230 +/- 23 0.010395 -0.146633 -0.145997 0.0906051 0.3096159 CAMK1D calcium/calmodulin-dependent protein kinase ID 5742 +/- 860 4749 +/- 865 8971 +/- 946 7264 +/- 308 0.000192 -0.43445 -0.136781 0.4668444 0.322584 CAMK2A calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha 1142 +/- 306 753 +/- 139 2093 +/- 147 1714 +/- 114 0.002798 -0.285894 -0.072636 0.2766242 0.1630859 CAMK2B calcium/calmodulin-dependent protein kinase (CaM kinase) II beta 8378 +/- 995 6517 +/- 684 9930 +/- 446 9486 +/- 334 0.002314 -0.158147 -0.446286 0.1291989 0.3175824 CAMK2N1 calcium/calmodulin-dependent protein kinase II inhibitor 1 1334 +/- 217 1125 +/- 205 2983 +/- 242 2989 +/- 145 8.05E-07 -0.500331 0.983502 0.2788314 0.7905494 CAMK2N2 calcium/calmodulin-dependent protein kinase II inhibitor 2 223 +/- 16 232 +/- 28 458 +/- 21 427 +/- 32 9.77E-06 0.7862696 -0.443863 0.860843 0.0652476 CAPN14 calpain 14 515 +/- 142 457 +/- 103 1121 +/- 120 810 +/- 208 0.004505 -0.745406 -0.238602 0.906264 0.7542111 CAPN7 calpain 7 580 +/- 29 674 +/- 27 693 +/- 37 800 +/- 39 0.004627 0.039422 0.081336 0.0466246 0.266771 CARF collaborates/cooperates with ARF (alternate reading frame) protein 269 +/- 10 254 +/- 13 331 +/- 17 273 +/- 18 0.037888 -0.405664 -0.042435 0.9848762 0.2328383 CARM1 coactivator-associated arginine methyltransferase 1 1526 +/- 57 1475 +/- 97 2331 +/- 125 2415 +/- 71 1.31E-06 -0.662596 0.577205 0.5637708 0.3021402 CASD1 CAS1 domain containing 1 1631 +/- 123 1528 +/- 125 1808 +/- 82 1784 +/- 126 0.000249 -0.569272 -0.87929 0.8673704 0.6750259 CAST1 CAZ-associated structural protein 3281 +/- 360 2648 +/- 269 4391 +/- 181 3526 +/- 194 0.000442 -0.19195 -0.010196 0.208326 0.0276119 CATSPER2Cation channel, sperm associated 2 1251 +/- 192 1531 +/- 138 1696 +/- 132 1731 +/- 115 0.024856 0.2654922 0.844114 0.4829183 0.6128966 CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3 270 +/- 31 270 +/- 34 456 +/- 32 421 +/- 33 0.001795 -0.99537 -0.466516 0.7951848 0.74031 CBLL1 Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1 113 +/- 15 138 +/- 11 160 +/- 9 149 +/- 13 0.023467 0.2033422 -0.535853 0.3451051 0.7470143 CBX3 chromobox homolog 3 (HP1 gamma homolog, Drosophila) 2091 +/- 138 2460 +/- 88 2478 +/- 74 2807 +/- 90 0.000466 0.0528484 0.021609 0.0976501 0.0574181

S2-genes differentially expressed across region.xls Page 4 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI CCBL1 cysteine conjugate-beta lyase; cytoplasmic (glutamine transaminase K, kyneurenine amin 94 +/- 15 147 +/- 21 187 +/- 29 204 +/- 29 0.003461 0.0726369 0.690722 0.0218178 0.2362619 CCDC104 coiled-coil domain containing 104 1930 +/- 93 1980 +/- 89 2238 +/- 65 2306 +/- 52 0.004092 0.7036113 0.430708 0.6125537 0.4844624 CCDC106 coiled-coil domain containing 106 825 +/- 51 823 +/- 38 996 +/- 13 1076 +/- 33 0.000201 -0.974663 0.069488 0.569547 0.1544263 CCDC109Acoiled-coil domain containing 109A 569 +/- 44 445 +/- 19 621 +/- 31 560 +/- 29 0.009885 -0.036178 -0.189661 0.0297814 0.12063 CCDC22 coiled-coil domain containing 22 256 +/- 15 289 +/- 14 333 +/- 6 355 +/- 28 0.000165 0.1451373 0.480868 0.0343588 0.2142034 CCDC46 coiled-coil domain containing 46 98 +/- 13 159 +/- 31 208 +/- 13 282 +/- 17 0.000554 0.1194535 0.009056 0.2527666 0.1569679 CCDC47 coiled-coil domain containing 47 1332 +/- 119 1261 +/- 75 1649 +/- 62 1703 +/- 40 3.6E-05 -0.628113 0.485883 0.9270622 0.4524718 CCDC53 coiled-coil domain containing 53 2279 +/- 84 2070 +/- 45 2483 +/- 66 2361 +/- 61 0.000809 -0.061328 -0.207288 0.2132139 0.2471815 CCDC62 coiled-coil domain containing 62 202 +/- 10 192 +/- 24 255 +/- 15 220 +/- 13 0.022727 -0.723421 -0.110854 0.5350947 0.1017136 CCDC64 coiled-coil domain containing 64 303 +/- 11 246 +/- 29 415 +/- 24 310 +/- 41 0.000978 -0.113729 -0.065624 0.0106806 0.0841138 CCDC71 coiled-coil domain containing 71 186 +/- 16 185 +/- 18 261 +/- 10 233 +/- 16 0.000646 -0.959453 -0.190742 0.9001207 0.5420564 CCDC73 Coiled-coil domain containing 73 148 +/- 13 193 +/- 19 208 +/- 24 243 +/- 22 0.000349 0.0771419 0.31368 0.4759757 0.9971332 CCDC74A coiled-coil domain containing 74A 613 +/- 51 646 +/- 93 640 +/- 36 1150 +/- 118 0.030966 0.7647319 0.010155 0.4369118 0.0075341 CCDC85A coiled-coil domain containing 85A 92 +/- 15 41 +/- 10 189 +/- 13 169 +/- 26 9.68E-05 -0.0203 -0.516734 0.0450543 0.794482 CCDC95 coiled-coil domain containing 95 936 +/- 37 859 +/- 28 1082 +/- 28 950 +/- 21 0.002123 -0.12682 -0.004763 0.0487537 0.0046589 CCNF cyclin F 133 +/- 13 125 +/- 9 143 +/- 17 196 +/- 13 0.025337 -0.623944 0.034926 0.3664781 0.1488046 CCNJL cyclin J-like 140 +/- 28 97 +/- 14 231 +/- 18 221 +/- 17 2.83E-05 -0.205966 -0.696316 0.542244 0.7534013 CCNK cyclin K 94 +/- 9 83 +/- 7 112 +/- 8 118 +/- 5 0.012638 -0.358048 0.519178 0.7239941 0.3823066 CD109 CD109 molecule 328 +/- 25 296 +/- 14 469 +/- 45 429 +/- 37 0.000521 -0.285965 -0.513863 0.3022911 0.5978264 CD44 CD44 molecule (Indian blood group) 390 +/- 49 579 +/- 97 642 +/- 74 919 +/- 44 0.000529 0.1243777 0.012964 0.2130658 0.0386421 CDC2L5 cell division cycle 2-like 5 (cholinesterase-related cell division controller) 154 +/- 15 189 +/- 18 254 +/- 19 238 +/- 25 0.00237 0.1611339 -0.614223 0.1396571 0.9833535 CDC42EP3CDC42 effector protein (Rho GTPase binding) 3 542 +/- 27 709 +/- 197 1566 +/- 202 1280 +/- 118 0.002988 0.4375089 -0.258786 0.920361 0.6431995 CDC42EP4CDC42 effector protein (Rho GTPase binding) 4 1927 +/- 191 2266 +/- 191 2361 +/- 146 2592 +/- 76 0.003363 0.2373062 0.200284 0.2947706 0.5421832 CDH2 cadherin 2, type 1, N-cadherin (neuronal) 810 +/- 57 761 +/- 34 909 +/- 44 800 +/- 28 0.042697 -0.480515 -0.067522 0.350174 0.0352823 CDK10 cyclin-dependent kinase (CDC2-like) 10 317 +/- 23 260 +/- 21 376 +/- 30 300 +/- 29 0.0455 -0.098302 -0.099045 0.0284414 0.1408962 CDK5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35) 3649 +/- 206 3034 +/- 85 4001 +/- 116 3504 +/- 169 0.04031 -0.029702 -0.043974 0.0157937 0.3073854 CDS2 CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2 268 +/- 25 253 +/- 20 315 +/- 30 357 +/- 19 0.020849 -0.643399 0.255582 0.5062351 0.5768903 CEBPD CCAAT/enhancer binding protein (C/EBP), delta 935 +/- 162 910 +/- 202 1864 +/- 174 1819 +/- 146 0.000387 -0.924942 -0.846859 0.9948041 0.8402012 CEBPZ CCAAT/enhancer binding protein zeta 199 +/- 29 139 +/- 16 273 +/- 28 215 +/- 25 0.000421 -0.109977 -0.150503 0.1083294 0.3433929 CECR2 cat eye syndrome chromosome region, candidate 2 185 +/- 10 254 +/- 43 502 +/- 51 532 +/- 21 2.89E-05 0.1727411 0.607575 0.1698566 0.4379265 CENTB2 centaurin, beta 2 1858 +/- 361 1644 +/- 455 3924 +/- 776 2263 +/- 292 0.003896 -0.721737 -0.089417 0.7361791 0.1530143 CENTB5 centaurin, beta 5 1430 +/- 52 1228 +/- 84 1951 +/- 111 1836 +/- 122 0.000185 -0.074093 -0.504068 0.0713182 0.8265509 CEP57 centrosomal protein 57kDa 423 +/- 44 326 +/- 24 490 +/- 24 410 +/- 42 0.016229 -0.088678 -0.143011 0.0644437 0.1963139 CEP68 centrosomal protein 68kDa 351 +/- 53 275 +/- 40 468 +/- 35 417 +/- 34 0.002646 -0.278426 -0.330937 0.4806711 0.9132289 CERK ceramide kinase 696 +/- 53 650 +/- 54 784 +/- 35 782 +/- 38 0.014076 -0.562579 -0.967478 0.3109122 0.3681514 CFI complement factor I 194 +/- 21 157 +/- 16 215 +/- 13 209 +/- 24 0.04605 -0.18989 -0.832641 0.3000795 0.9297855 CGI-69 CGI-69 protein 557 +/- 15 587 +/- 32 593 +/- 25 620 +/- 38 0.025048 0.432272 0.560359 0.9092316 0.9988542 CHAC1 ChaC, cation transport regulator-like 1 (E. coli) 407 +/- 29 288 +/- 46 453 +/- 22 383 +/- 54 0.03788 -0.057374 -0.280775 0.0015119 0.0879659 CHAC2 ChaC, cation transport regulator-like 2 (E. coli) 224 +/- 21 268 +/- 12 317 +/- 26 293 +/- 30 0.020588 0.1038168 -0.565578 0.0669849 0.3067155 CHCHD3 coiled-coil-helix-coiled-coil-helix domain containing 3 2026 +/- 56 2199 +/- 30 2231 +/- 90 2360 +/- 104 0.004669 0.0263417 0.377636 0.0127559 0.5219367 CHCHD6 coiled-coil-helix-coiled-coil-helix domain containing 6 1282 +/- 127 1172 +/- 74 1401 +/- 55 1444 +/- 71 0.017171 -0.472891 0.649748 0.3828076 0.7174202 CHD9 chromodomain helicase DNA binding protein 9 223 +/- 26 284 +/- 14 310 +/- 19 323 +/- 28 0.00303 0.0745458 0.708413 0.0921462 0.2791218 CHGA chromogranin A (parathyroid secretory protein 1) 1586 +/- 138 1605 +/- 137 2124 +/- 206 1846 +/- 125 0.00198 0.9243539 -0.282052 0.9940694 0.4290749 CHKA choline kinase alpha 607 +/- 41 586 +/- 44 764 +/- 36 646 +/- 32 0.030471 -0.738949 -0.035292 0.8615465 0.0564405 CHST1 carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 6002 +/- 405 5582 +/- 554 8515 +/- 319 8492 +/- 398 5.29E-07 -0.555316 -0.966527 0.2428978 0.4775345

S2-genes differentially expressed across region.xls Page 5 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI CHST12 carbohydrate (chondroitin 4) sulfotransferase 12 604 +/- 59 580 +/- 36 767 +/- 25 729 +/- 34 0.000345 -0.744886 -0.38913 0.9187087 0.961015 CHST7 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7 268 +/- 17 266 +/- 8 379 +/- 11 384 +/- 14 4.28E-07 -0.913632 0.778645 0.588775 0.8120931 CIB2 calcium and integrin binding family member 2 601 +/- 121 688 +/- 196 1797 +/- 98 2311 +/- 302 8.72E-07 0.7157727 0.168871 0.71586 0.4907188 CIC capicua homolog (Drosophila) 2030 +/- 162 2055 +/- 125 3575 +/- 166 3066 +/- 138 3.82E-05 0.9079011 -0.042879 0.6272452 0.1751269 CKAP4 cytoskeleton-associated protein 4 204 +/- 24 208 +/- 23 322 +/- 15 280 +/- 17 0.001174 0.9160312 -0.098186 0.8731486 0.0295255 CLASP1 cytoplasmic linker associated protein 1 2136 +/- 118 2302 +/- 115 2430 +/- 99 2422 +/- 66 0.025048 0.3364966 -0.947592 0.665754 0.6712575 CLDN12 claudin 12 1033 +/- 55 999 +/- 54 1262 +/- 42 1127 +/- 73 0.016263 -0.665938 -0.155331 0.44356 0.2265472 CLEC2L C-type lectin domain family 2, member L 1172 +/- 148 1076 +/- 84 1876 +/- 62 1736 +/- 78 0.000223 -0.586729 -0.200326 0.4398249 0.2982463 CLIC2 chloride intracellular channel 2 216 +/- 8 204 +/- 11 253 +/- 13 217 +/- 20 0.036701 -0.395662 -0.169803 0.3587369 0.1787874 CLIPR-59 CLIP-170-related protein 9682 +/- 312 9221 +/- 283 10098 +/- 459 10336 +/- 205 0.021261 -0.298931 0.650289 0.033528 0.6103093 CLSTN1 calsyntenin 1 10328 +/- 379 10245 +/- 671 11462 +/- 390 11985 +/- 352 0.00441 -0.91705 0.346355 0.4198951 0.5355616 CLTB clathrin, light polypeptide (Lcb) 1137 +/- 74 975 +/- 45 1787 +/- 81 1662 +/- 80 3.7E-08 -0.099012 -0.298477 0.1114392 0.2881023 CNIH2 cornichon homolog 2 (Drosophila) 4845 +/- 1276 3557 +/- 721 5938 +/- 434 6322 +/- 120 0.020794 -0.405364 0.427508 0.3805487 0.363364 CNNM1 cyclin M1 1123 +/- 60 1055 +/- 59 1264 +/- 76 1334 +/- 49 0.008325 -0.436617 0.463508 0.1842889 0.2939187 CNNM2 cyclin M2 138 +/- 14 126 +/- 10 173 +/- 5 173 +/- 20 0.009837 -0.503143 0.989428 0.5082101 0.3084902 CNNM4 cyclin M4 725 +/- 27 725 +/- 65 820 +/- 58 939 +/- 107 0.030544 0.9984802 0.362688 0.9856083 0.4626591 CNO cappuccino homolog (mouse) 618 +/- 47 639 +/- 24 696 +/- 28 720 +/- 24 0.025041 0.6941246 0.531721 0.872911 0.8813339 CNOT4 CCR4-NOT transcription complex, subunit 4 154 +/- 17 203 +/- 23 198 +/- 14 236 +/- 26 0.045992 0.1180435 0.24617 0.1207696 0.1818664 CNOT6 CCR4-NOT transcription complex, subunit 6 294 +/- 19 256 +/- 19 427 +/- 33 359 +/- 33 0.001479 -0.179628 -0.177625 0.4135085 0.5111732 CNOT7 CCR4-NOT transcription complex, subunit 7 155 +/- 23 135 +/- 21 176 +/- 17 197 +/- 16 0.041018 -0.529895 0.382661 0.2880574 0.7030476 COBRA1 cofactor of BRCA1 1580 +/- 53 1459 +/- 54 1712 +/- 117 1680 +/- 76 0.043338 -0.139631 -0.827083 0.0286412 0.8131698 COG2 Component of oligomeric golgi complex 2 120 +/- 4 171 +/- 15 148 +/- 17 181 +/- 22 0.046117 0.0163734 0.26106 0.035707 0.4117899 COIL coilin 391 +/- 11 442 +/- 36 416 +/- 14 457 +/- 23 0.004235 0.2181572 0.166501 0.3938599 0.7520259 COL11A1 Collagen, type XI, alpha 1 242 +/- 36 233 +/- 53 334 +/- 77 299 +/- 25 0.008343 -0.890714 -0.683071 0.9713261 0.7598363 COQ10A coenzyme Q10 homolog A (S. cerevisiae) 1795 +/- 102 1613 +/- 139 2087 +/- 128 2110 +/- 56 0.00078 -0.31693 0.877575 0.1218872 0.7051987 CORO7 coronin 7 1060 +/- 57 830 +/- 78 1137 +/- 43 970 +/- 97 0.053474 -0.040219 -0.170909 0.1279173 0.5328214 COVA1 cytosolic ovarian carcinoma antigen 1 368 +/- 18 319 +/- 18 431 +/- 30 438 +/- 49 0.002276 -0.089331 0.909505 0.0664763 0.9379283 COX15 COX15 homolog, cytochrome c oxidase assembly protein (yeast) 352 +/- 99 165 +/- 17 388 +/- 108 209 +/- 22 0.049281 -0.119876 -0.159232 0.1202296 0.1603139 COX18 COX18 cytochrome c oxidase assembly homolog (S. cerevisiae) 768 +/- 30 805 +/- 66 969 +/- 79 995 +/- 52 0.000911 0.6230822 0.793104 0.8085991 0.7805394 CPEB3 cytoplasmic polyadenylation element binding protein 3 804 +/- 59 910 +/- 76 1183 +/- 88 1022 +/- 26 0.004859 0.2998087 -0.131368 0.2571263 0.4687073 CPEB4 cytoplasmic polyadenylation element binding protein 4 4799 +/- 191 4286 +/- 268 5536 +/- 303 4833 +/- 381 0.035796 -0.153788 -0.186138 0.3958748 0.5125972 CPNE3 copine III 231 +/- 31 263 +/- 45 344 +/- 38 339 +/- 10 0.003274 0.5662515 -0.905409 0.9872536 0.6483312 CPNE6 copine VI (neuronal) 4669 +/- 606 3745 +/- 532 5577 +/- 183 5452 +/- 182 0.005525 -0.27879 -0.640193 0.2058663 0.3720702 CPSF2 cleavage and polyadenylation specific factor 2, 100kDa 526 +/- 47 668 +/- 60 737 +/- 35 880 +/- 49 0.000273 0.0943332 0.046656 0.0480927 0.0324146 CRB1 crumbs homolog 1 (Drosophila) 582 +/- 81 470 +/- 73 756 +/- 152 570 +/- 91 0.01675 -0.331403 -0.322583 0.3216722 0.3430662 CREG2 cellular repressor of E1A-stimulated genes 2 846 +/- 121 830 +/- 83 1680 +/- 147 1811 +/- 202 1.54E-06 -0.914142 0.61356 0.8321873 0.6283235 CRELD1 cysteine-rich with EGF-like domains 1 1059 +/- 51 1176 +/- 83 1194 +/- 37 1408 +/- 109 0.011576 0.260255 0.121994 0.7935709 0.6368704 CRHR1 corticotropin releasing hormone receptor 1 111 +/- 17 127 +/- 20 184 +/- 13 194 +/- 13 0.000804 0.5588239 0.598013 0.7764756 0.6334302 CRI2 CREBBP/EP300 inhibitor 2 2677 +/- 69 2462 +/- 64 2961 +/- 148 2988 +/- 133 0.001303 -0.045566 0.896077 0.0134155 0.9560909 CRIP2 cysteine-rich protein 2 1382 +/- 134 1325 +/- 147 2343 +/- 159 2154 +/- 201 0.001191 -0.781721 -0.481786 0.8060977 0.8227177 CRK v-crk sarcoma virus CT10 oncogene homolog (avian) 1006 +/- 105 1180 +/- 70 1192 +/- 75 1386 +/- 52 0.039045 0.201792 0.064645 0.4156511 0.0839076 CRLS1 cardiolipin synthase 1 588 +/- 53 627 +/- 37 696 +/- 30 755 +/- 37 0.012277 0.564588 0.24712 0.5340446 0.2880741 CRSP7 cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa 128 +/- 17 155 +/- 13 208 +/- 16 237 +/- 19 0.001635 0.2374435 0.275427 0.5756172 0.0582313 CRSP8 Cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa 434 +/- 20 498 +/- 71 603 +/- 36 587 +/- 58 0.007796 0.4151226 -0.822411 0.2665485 0.3816379 CRTAP cartilage associated protein 291 +/- 21 263 +/- 16 339 +/- 21 323 +/- 30 0.017178 -0.317219 -0.677885 0.214606 0.1597177

S2-genes differentially expressed across region.xls Page 6 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI CRTC1 CREB regulated transcription coactivator 1 694 +/- 49 669 +/- 39 886 +/- 54 816 +/- 49 0.00174 -0.697372 -0.355334 0.6067761 0.4343661 CRY2 cryptochrome 2 (photolyase-like) 1270 +/- 141 1238 +/- 108 1549 +/- 101 1570 +/- 118 0.00779 -0.860285 0.895037 0.6416839 0.7163026 CRYL1 crystallin, lambda 1 1943 +/- 176 2157 +/- 185 2168 +/- 123 2251 +/- 109 0.039096 0.4199032 0.62659 0.2927045 0.8211372 CSDC2 cold shock domain containing C2, RNA binding 680 +/- 46 613 +/- 33 1125 +/- 176 708 +/- 42 0.029274 -0.263454 -0.06501 0.1841661 0.1956686 CSNK1G1 casein kinase 1, gamma 1 1177 +/- 134 1044 +/- 127 1871 +/- 108 1783 +/- 152 0.000222 -0.48549 -0.648574 0.5988358 0.6995579 CSNK1G2 casein kinase 1, gamma 2 464 +/- 48 332 +/- 27 622 +/- 27 496 +/- 28 0.001863 -0.044697 -0.010409 0.0355452 0.0068422 CSPG5 chondroitin sulfate proteoglycan 5 (neuroglycan C) 645 +/- 130 684 +/- 71 783 +/- 137 842 +/- 34 0.04332 0.7995659 0.689491 0.9793079 0.8222223 CSPG6 chondroitin sulfate proteoglycan 6 (bamacan) 905 +/- 42 999 +/- 35 1043 +/- 23 1095 +/- 26 0.006321 0.1144404 0.173763 0.1777793 0.7387256 CTBP1 C-terminal binding protein 1 2888 +/- 204 2604 +/- 172 3515 +/- 93 3484 +/- 319 7.83E-05 -0.311226 -0.930297 0.6742056 0.2127629 CTDSPL CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-lik 1200 +/- 112 1139 +/- 107 1460 +/- 104 1482 +/- 75 0.039304 -0.703027 0.872239 0.395167 0.9769086 CTDSPL2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 152 +/- 18 148 +/- 23 211 +/- 12 220 +/- 11 0.002249 -0.89057 0.613072 0.5318435 0.8634848 CTSD cathepsin D (lysosomal aspartyl peptidase) 96 +/- 9 129 +/- 13 113 +/- 15 163 +/- 20 0.037274 0.0665338 0.076052 0.3399761 0.392234 CUGBP2 CUG triplet repeat, RNA binding protein 2 1263 +/- 229 2289 +/- 319 1772 +/- 270 3161 +/- 347 0.008986 0.028023 0.013492 0.2405286 0.0448164 CUTL1 Cut-like 1, CCAAT displacement protein (Drosophila) 378 +/- 77 485 +/- 43 522 +/- 91 525 +/- 41 0.006217 0.257602 0.979451 0.2423987 0.8681033 CX3CL1 chemokine (C-X3-C motif) ligand 1 1624 +/- 152 1601 +/- 101 2323 +/- 188 2403 +/- 164 0.000276 -0.900153 0.755045 0.8558982 0.7205099 CXCL12 chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) 703 +/- 160 527 +/- 166 1328 +/- 203 1073 +/- 141 0.009785 -0.464018 -0.331816 0.4080976 0.4173706 CXXC5 CXXC finger 5 994 +/- 34 1117 +/- 49 1164 +/- 73 1189 +/- 50 0.031187 0.0700206 0.784176 0.041982 0.7165496 CYB5B cytochrome b5 type B (outer mitochondrial membrane) 133 +/- 13 119 +/- 10 172 +/- 12 184 +/- 15 0.000387 -0.419336 0.53555 0.2457467 0.7379613 CYB5R4 cytochrome b5 reductase 4 572 +/- 78 466 +/- 12 883 +/- 67 901 +/- 23 1.41E-05 -0.232518 0.80378 0.3417509 0.7785196 CYFIP2 cytoplasmic FMR1 interacting protein 2 7939 +/- 518 7026 +/- 654 9734 +/- 416 9881 +/- 227 9.99E-05 -0.300743 0.764292 0.1419411 0.9954549 CYGB cytoglobin 435 +/- 35 481 +/- 67 817 +/- 75 758 +/- 65 0.002032 0.5689869 -0.562951 0.9473438 0.7994819 CYHR1 cysteine/histidine-rich 1 407 +/- 30 454 +/- 11 482 +/- 39 573 +/- 18 0.005119 0.18949 0.070349 0.3292366 0.0866017 CYP1A2 cytochrome P450, family 1, subfamily A, polypeptide 2 392 +/- 71 435 +/- 15 516 +/- 61 515 +/- 45 0.027864 0.5762672 -0.994071 0.7181627 0.8737705 CYP51A1 cytochrome P450, family 51, subfamily A, polypeptide 1 944 +/- 116 942 +/- 122 1222 +/- 149 1322 +/- 110 0.008165 -0.990052 0.601357 0.6060112 0.9310146 D15Wsu75 DNA segment, Chr 15, Wayne State University 75, expressed 91 +/- 11 85 +/- 13 120 +/- 9 131 +/- 7 0.0032 -0.763386 0.32525 0.716918 0.3790334 DAB1 disabled homolog 1 (Drosophila) 460 +/- 76 362 +/- 64 654 +/- 77 658 +/- 110 0.005479 -0.350083 0.979193 0.2728483 0.6631248 DARC Duffy blood group, chemokine receptor 193 +/- 21 183 +/- 27 223 +/- 9 224 +/- 22 0.02157 -0.792417 0.988793 0.9473223 0.3797672 DARS2 aspartyl-tRNA synthetase 2 (mitochondrial) 227 +/- 13 222 +/- 17 290 +/- 14 260 +/- 15 0.004032 -0.837741 -0.184773 0.4260238 0.154951 DCAMKL3 doublecortin and CaM kinase-like 3 109 +/- 33 103 +/- 30 307 +/- 29 309 +/- 45 0.000967 -0.900873 0.976056 0.9106733 0.5100497 DCP2 DCP2 decapping enzyme homolog (S. cerevisiae) 1397 +/- 130 1177 +/- 146 1797 +/- 108 1546 +/- 28 0.010662 -0.284266 -0.067186 0.2466468 0.1278582 DDA1 DDA1 732 +/- 46 716 +/- 45 779 +/- 25 794 +/- 29 0.044076 -0.801424 0.693934 0.7946806 0.5668183 DDAH2 dimethylarginine dimethylaminohydrolase 2 1020 +/- 65 913 +/- 36 1130 +/- 29 1042 +/- 58 0.030592 -0.18735 -0.227553 0.0553096 0.0946574 DDX27 DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 174 +/- 17 135 +/- 12 208 +/- 13 187 +/- 11 0.002145 -0.089149 -0.244527 0.3361899 0.2221413 DDX5 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 6120 +/- 241 6129 +/- 153 6713 +/- 153 6541 +/- 98 0.009559 0.9746182 -0.370793 0.4789319 0.771724 DEAF1 deformed epidermal autoregulatory factor 1 (Drosophila) 2565 +/- 177 2227 +/- 171 2971 +/- 112 2869 +/- 133 0.001992 -0.201086 -0.575484 0.0484783 0.2487354 DENND4A DENN/MADD domain containing 4A 532 +/- 22 507 +/- 27 580 +/- 34 590 +/- 33 0.03551 -0.497617 0.831348 0.4205501 0.9938668 DENND4C DENN/MADD domain containing 4C 820 +/- 63 974 +/- 25 964 +/- 32 1109 +/- 62 0.01039 0.0583166 0.081732 0.0165599 0.0189646 DEXI dexamethasone-induced transcript 1477 +/- 130 1606 +/- 45 1674 +/- 75 1776 +/- 85 0.031108 0.3867041 0.39338 0.2644254 0.9119021 DFFA DNA fragmentation factor, 45kDa, alpha polypeptide 632 +/- 34 537 +/- 27 694 +/- 27 593 +/- 28 0.028154 -0.054305 -0.031207 0.1962728 0.2976787 DGCR2 DiGeorge syndrome critical region gene 2 193 +/- 33 205 +/- 29 345 +/- 33 363 +/- 20 0.000107 0.7792145 0.645864 0.9385848 0.9617381 DGKB diacylglycerol kinase, beta 90kDa 158 +/- 21 119 +/- 19 253 +/- 37 177 +/- 22 0.006968 -0.19859 -0.117655 0.4566594 0.2105405 DHCR7 7-dehydrocholesterol reductase 440 +/- 49 518 +/- 33 575 +/- 44 595 +/- 31 0.011554 0.2184644 0.713567 0.4011856 0.954846 DHX30 DEAH (Asp-Glu-Ala-His) box polypeptide 30 1017 +/- 53 1151 +/- 57 1245 +/- 83 1161 +/- 43 0.04522 0.1170477 -0.396867 0.0335157 0.5880852 DHX33 DEAH (Asp-Glu-Ala-His) box polypeptide 33 97 +/- 25 75 +/- 8 129 +/- 17 161 +/- 69 0.017251 -0.449436 0.669206 0.8623757 0.0422655 DHX57 DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 537 +/- 46 592 +/- 42 699 +/- 24 771 +/- 27 0.002757 0.3940215 0.080692 0.4733718 0.0625953

S2-genes differentially expressed across region.xls Page 7 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI DIP death-inducing-protein 965 +/- 62 835 +/- 40 1120 +/- 63 939 +/- 66 0.006587 -0.11048 -0.080808 0.2459481 0.0600143 DIP2C DIP2 disco-interacting protein 2 homolog C (Drosophila) 336 +/- 30 418 +/- 15 445 +/- 23 481 +/- 54 0.02934 0.0454459 0.56088 0.0322439 0.2303166 DKFZP434 DKFZP434B0335 protein 1010 +/- 70 889 +/- 105 1390 +/- 56 1264 +/- 72 1.77E-05 -0.362325 -0.204199 0.3256262 0.0372663 DKK3 dickkopf homolog 3 (Xenopus laevis) 662 +/- 94 813 +/- 76 1201 +/- 170 970 +/- 100 0.016658 0.2381366 -0.276984 0.0777283 0.2230512 DLGAP2 discs, large (Drosophila) homolog-associated protein 2 456 +/- 23 437 +/- 25 554 +/- 43 586 +/- 54 0.010298 -0.590922 0.654245 0.3748396 0.7508683 DMWD dystrophia myotonica-containing WD repeat motif 852 +/- 123 758 +/- 79 1066 +/- 46 1069 +/- 147 0.011512 -0.535811 0.986847 0.3373324 0.656268 DMXL2 Dmx-like 2 5689 +/- 431 5295 +/- 435 7966 +/- 288 7474 +/- 325 6.13E-07 -0.534754 -0.287522 0.5348546 0.2484567 DNA2L DNA2 DNA replication helicase 2-like (yeast) 152 +/- 18 166 +/- 13 190 +/- 8 180 +/- 15 0.018567 0.5618428 -0.595505 0.6752629 0.3368106 DNAJB14 DnaJ (Hsp40) homolog, subfamily B, member 14 745 +/- 75 583 +/- 44 992 +/- 140 720 +/- 97 0.003076 -0.099763 -0.145233 0.2740894 0.4450893 DNAJB4 DnaJ (Hsp40) homolog, subfamily B, member 4 249 +/- 44 273 +/- 40 286 +/- 16 415 +/- 45 0.048566 0.6888496 0.042426 0.6548772 0.1443555 DNAJC12 DnaJ (Hsp40) homolog, subfamily C, member 12 465 +/- 101 368 +/- 82 634 +/- 98 534 +/- 82 0.001016 -0.472979 -0.449626 0.2282365 0.2423587 DNAJC15 DnaJ (Hsp40) homolog, subfamily C, member 15 1031 +/- 44 970 +/- 62 1158 +/- 83 1039 +/- 74 0.015752 -0.442216 -0.311672 0.4514713 0.1567617 DNAJC18 DnaJ (Hsp40) homolog, subfamily C, member 18 191 +/- 25 173 +/- 20 259 +/- 21 252 +/- 23 0.016433 -0.582231 -0.835489 0.9652133 0.914995 DNAJC5 DnaJ (Hsp40) homolog, subfamily C, member 5 2366 +/- 278 2055 +/- 187 3214 +/- 219 2977 +/- 149 4.45E-05 -0.377171 -0.395941 0.2683837 0.2709331 DNAJC6 DnaJ (Hsp40) homolog, subfamily C, member 6 140 +/- 15 221 +/- 38 168 +/- 9 226 +/- 44 0.026514 0.0908478 0.26663 0.0211226 0.0375376 DNAL4 dynein, axonemal, light polypeptide 4 705 +/- 71 658 +/- 44 767 +/- 42 865 +/- 25 0.01005 -0.590838 0.083973 0.2457717 0.197536 DNER delta-notch-like EGF repeat-containing transmembrane 10019 +/- 428 10769 +/- 581 11403 +/- 460 12362 +/- 358 0.000776 0.3249559 0.135095 0.7686456 0.2154026 DOC2A double C2-like domains, alpha 1168 +/- 131 999 +/- 81 1474 +/- 61 1333 +/- 72 0.001571 -0.302181 -0.171678 0.1868709 0.6785955 DOCK11 dedicator of cytokinesis 11 252 +/- 34 340 +/- 20 340 +/- 23 371 +/- 36 0.005945 0.0554595 0.504763 0.166568 0.6281943 DOCK4 dedicator of cytokinesis 4 83 +/- 24 256 +/- 47 145 +/- 31 379 +/- 38 0.004588 0.0118613 0.001341 0.0689508 0.102621 DONSON downstream neighbor of SON 391 +/- 20 421 +/- 12 456 +/- 14 469 +/- 18 0.001059 0.2336859 0.596983 0.2772727 0.4314983 DPF1 D4, zinc and double PHD fingers family 1 1631 +/- 91 1083 +/- 88 1997 +/- 111 1529 +/- 115 0.001532 -0.001498 -0.017396 0.0084248 0.1077978 DPH1 DPH1 homolog (S. cerevisiae) 1108 +/- 78 1033 +/- 107 1407 +/- 137 1177 +/- 49 0.002102 -0.585895 -0.163628 0.720144 0.3985284 DPH2 DPH2 homolog (S. cerevisiae) 436 +/- 19 388 +/- 22 458 +/- 21 458 +/- 28 0.034739 -0.138192 0.992342 0.2010963 0.6862508 DPY19L3 dpy-19-like 3 (C. elegans) 1853 +/- 163 1900 +/- 215 2092 +/- 93 2260 +/- 81 0.049851 0.8663101 0.204321 0.9444893 0.4277343 DRD1IP dopamine receptor D1 interacting protein 4415 +/- 228 3841 +/- 274 6342 +/- 328 6147 +/- 161 8.98E-06 -0.139716 -0.609595 0.0883924 0.4570165 DTNBP1 dystrobrevin binding protein 1 2536 +/- 191 2610 +/- 141 2864 +/- 166 3189 +/- 244 0.002722 0.7609774 0.306114 0.9993805 0.4100094 DTX3 deltex 3 homolog (Drosophila) 397 +/- 50 340 +/- 59 552 +/- 34 512 +/- 44 0.00556 -0.477097 -0.490723 0.42899 0.4698627 DUSP14 dual specificity phosphatase 14 990 +/- 110 838 +/- 108 1439 +/- 43 1252 +/- 51 0.001272 -0.347324 -0.021931 0.3335945 0.1388966 DUSP22 Dual specificity phosphatase 22 574 +/- 24 595 +/- 42 651 +/- 37 723 +/- 24 0.011773 0.6723092 0.146268 0.6205026 0.2387273 DUSP23 dual specificity phosphatase 23 1324 +/- 83 1107 +/- 72 1378 +/- 31 1385 +/- 41 0.045857 -0.078192 0.896273 0.1645791 0.6684534 DVL1 dishevelled, dsh homolog 1 (Drosophila) 3587 +/- 231 3544 +/- 263 4279 +/- 294 4258 +/- 179 0.008718 -0.905066 -0.952509 0.5087976 0.6858871 DVL3 dishevelled, dsh homolog 3 (Drosophila) 658 +/- 28 719 +/- 33 730 +/- 31 776 +/- 43 0.023519 0.1909086 0.409533 0.0701807 0.4355523 DYNC2H1 dynein, cytoplasmic 2, heavy chain 1 508 +/- 62 728 +/- 72 644 +/- 82 957 +/- 115 0.003354 0.0439453 0.060176 0.2626924 0.3443156 DYRK1B dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B 532 +/- 30 487 +/- 26 841 +/- 50 765 +/- 43 1.26E-06 -0.280948 -0.279783 0.243966 0.3933199 DZIP1 DAZ interacting protein 1 1130 +/- 99 875 +/- 66 1406 +/- 101 904 +/- 129 0.050224 -0.060364 -0.015726 0.1075366 0.0672343 DZIP1L DAZ interacting protein 1-like 133 +/- 14 134 +/- 14 213 +/- 16 147 +/- 7 0.00727 0.975474 -0.006561 0.9479433 0.0103985 DZIP3 zinc finger DAZ interacting protein 3 497 +/- 49 471 +/- 55 668 +/- 42 679 +/- 54 0.001176 -0.73415 0.87964 0.5371562 0.4316046 EAF1 ELL associated factor 1 709 +/- 48 801 +/- 62 865 +/- 41 947 +/- 27 0.003573 0.268006 0.125524 0.6984566 0.4632446 ECAT8 ES cell associated transcript 8 328 +/- 108 313 +/- 88 822 +/- 55 656 +/- 136 0.001692 -0.918819 -0.305837 0.9664477 0.962472 ECE2 endothelin converting enzyme 2 941 +/- 140 1072 +/- 116 1417 +/- 106 1392 +/- 85 0.014156 0.4874433 -0.85908 0.7478143 0.5309516 ECHDC3 enoyl Coenzyme A hydratase domain containing 3 401 +/- 25 467 +/- 64 527 +/- 73 557 +/- 60 0.007072 0.3730127 0.754636 0.511956 0.7472365 ECM1 extracellular matrix protein 1 346 +/- 61 521 +/- 76 736 +/- 48 984 +/- 94 7.77E-06 0.1026941 0.057273 0.1711863 0.3533547 ECRG4 esophageal cancer related gene 4 protein 529 +/- 11 661 +/- 53 803 +/- 58 778 +/- 126 0.00793 0.0538984 -0.863526 0.0081832 0.3276857 EDIL3 EGF-like repeats and discoidin I-like domains 3 291 +/- 46 446 +/- 55 477 +/- 58 560 +/- 72 0.012705 0.0581498 0.393761 0.0063375 0.0860742

S2-genes differentially expressed across region.xls Page 8 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI EDNRB endothelin receptor type B 673 +/- 94 582 +/- 62 937 +/- 97 716 +/- 58 0.00781 -0.439832 -0.08722 0.2887409 0.0297193 EFCBP1 EF-hand calcium binding protein 1 121 +/- 11 170 +/- 24 211 +/- 30 210 +/- 35 0.017398 0.1105925 -0.989858 0.5250665 0.5514518 EFHA2 EF-hand domain family, member A2 3623 +/- 194 3050 +/- 248 4234 +/- 119 3864 +/- 96 0.006972 -0.100358 -0.038748 0.0544414 0.2160947 EFNB3 ephrin-B3 290 +/- 36 225 +/- 36 614 +/- 34 584 +/- 47 1.71E-05 -0.233242 -0.624008 0.2287089 0.7365298 EGLN1 egl nine homolog 1 (C. elegans) 599 +/- 67 426 +/- 24 799 +/- 81 561 +/- 31 0.013278 -0.050058 -0.031492 0.0742366 0.0520918 EGLN2 egl nine homolog 2 (C. elegans) 198 +/- 34 157 +/- 24 231 +/- 12 268 +/- 10 0.022033 -0.34357 0.04326 0.445131 0.4164312 EHBP1 EH domain binding protein 1 107 +/- 15 87 +/- 15 184 +/- 14 154 +/- 11 0.000497 -0.391006 -0.132182 0.4230867 0.1493682 EHD3 EH-domain containing 3 2227 +/- 172 1963 +/- 177 2875 +/- 148 2928 +/- 145 6.35E-05 -0.311007 0.80588 0.1501196 0.5372698 EHMT1 euchromatic histone-lysine N-methyltransferase 1 821 +/- 47 849 +/- 43 982 +/- 9 938 +/- 44 0.009595 0.6746315 -0.375558 0.7973994 0.217701 EID-3 EID-2-like inhibitor of differentiation-3 1170 +/- 82 1234 +/- 63 1297 +/- 45 1499 +/- 44 0.003591 0.5512022 0.010926 0.8176413 0.0879027 EIF2C4 Eukaryotic translation initiation factor 2C, 4 170 +/- 17 196 +/- 12 237 +/- 11 247 +/- 13 0.001749 0.236178 0.579522 0.1128747 0.539488 EIF2S3 eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa 554 +/- 55 594 +/- 35 656 +/- 54 692 +/- 61 0.000368 0.5527542 0.668271 0.4860182 0.7889089 EIF4G1 eukaryotic translation initiation factor 4 gamma, 1 235 +/- 38 191 +/- 18 279 +/- 22 320 +/- 20 0.003886 -0.334348 0.191017 0.3836689 0.1165018 EIF4G2 eukaryotic translation initiation factor 4 gamma, 2 153 +/- 23 166 +/- 18 320 +/- 72 280 +/- 29 0.004565 0.6761395 -0.625765 0.9352234 0.7651205 EIF5A eukaryotic translation initiation factor 5A 172 +/- 16 277 +/- 44 235 +/- 19 930 +/- 466 0.001977 0.0942463 0.210331 0.1928354 0.3713673 ELAC1 elaC homolog 1 (E. coli) 257 +/- 16 328 +/- 22 295 +/- 19 351 +/- 12 0.005793 0.0277609 0.037583 0.0035732 0.0142275 ELAVL3 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) 189 +/- 16 110 +/- 15 287 +/- 18 170 +/- 21 0.001759 -0.005034 -0.002436 0.013616 0.031892 ELF2 E74-like factor 2 (ets domain transcription factor) 138 +/- 9 166 +/- 11 197 +/- 6 171 +/- 17 0.024871 0.0838396 -0.19985 0.0171331 0.6475829 ELK1 ELK1, member of ETS oncogene family 717 +/- 108 657 +/- 98 1501 +/- 60 1575 +/- 136 2.82E-05 -0.688073 0.634924 0.4987335 0.9619796 ELMOD1 ELMO/CED-12 domain containing 1 5572 +/- 627 4657 +/- 660 7451 +/- 421 6427 +/- 390 0.009367 -0.338393 -0.107837 0.3466935 0.3386839 ELN elastin (supravalvular aortic stenosis, Williams-Beuren syndrome) 192 +/- 17 162 +/- 23 235 +/- 18 259 +/- 25 0.035427 -0.332852 0.467104 0.1283553 0.3257394 EML4 echinoderm microtubule associated protein like 4 276 +/- 12 333 +/- 21 362 +/- 36 401 +/- 18 0.002134 0.0464792 0.35486 0.0068409 0.5067077 EMX2 empty spiracles homolog 2 (Drosophila) 860 +/- 116 836 +/- 85 1476 +/- 75 1238 +/- 46 0.000166 -0.874806 -0.027446 0.900048 0.0081431 ENAH enabled homolog (Drosophila) 1733 +/- 261 2281 +/- 99 2232 +/- 238 2833 +/- 118 0.001729 0.0946837 0.057042 0.1828381 0.2257849 ENTPD3 ectonucleoside triphosphate diphosphohydrolase 3 123 +/- 12 126 +/- 13 163 +/- 19 189 +/- 23 0.018 0.8719711 0.400683 0.5841725 0.5645495 EPB41L1 erythrocyte membrane protein band 4.1-like 1 1223 +/- 101 1109 +/- 46 1374 +/- 63 1354 +/- 84 0.017996 -0.340198 -0.860587 0.1554998 0.9936991 EPDR1 ependymin related protein 1 (zebrafish) 3508 +/- 458 3845 +/- 122 4080 +/- 335 4663 +/- 230 0.00328 0.5058147 0.187223 0.332857 0.3547092 EPHA4 EPH receptor A4 577 +/- 137 322 +/- 39 807 +/- 161 909 +/- 151 0.02243 -0.126746 0.655593 0.1410946 0.9201381 EPHA7 EPH receptor A7 262 +/- 30 171 +/- 33 462 +/- 88 499 +/- 58 0.002215 -0.069329 0.731956 0.1151844 0.9413568 EPM2AIP1 EPM2A (laforin) interacting protein 1 2931 +/- 139 3229 +/- 109 3423 +/- 144 3492 +/- 127 0.004493 0.1249675 0.729232 0.1119277 0.4417082 EPS15L1 Epidermal growth factor receptor pathway substrate 15-like 1 180 +/- 22 143 +/- 17 279 +/- 57 215 +/- 47 0.003892 -0.203355 -0.409016 0.3931495 0.4727973 ERCC2 excision repair cross-complementing rodent repair deficiency, complementation group 2 (x 583 +/- 12 530 +/- 34 609 +/- 18 621 +/- 42 0.01766 -0.187647 0.807246 0.0028255 0.4710394 ERH enhancer of rudimentary homolog (Drosophila) 5444 +/- 236 5908 +/- 260 6027 +/- 113 6411 +/- 139 0.002513 0.2162074 0.06389 0.2280333 0.2420871 ETF1 eukaryotic translation termination factor 1 243 +/- 4 206 +/- 11 313 +/- 29 253 +/- 16 0.004699 -0.015712 -0.113144 0.0736888 0.1713913 EVL Enah/Vasp-like 119 +/- 27 136 +/- 18 222 +/- 18 218 +/- 14 0.001608 0.6146204 -0.884942 0.9700323 0.4287214 EZH2 Enhancer of zeste homolog 2 (Drosophila) 117 +/- 5 104 +/- 11 129 +/- 9 134 +/- 9 0.045748 -0.337857 0.694433 0.1457552 0.7307891 FAAH fatty acid amide hydrolase 921 +/- 103 1040 +/- 105 1172 +/- 52 1240 +/- 41 0.000843 0.4358339 0.327389 0.5863743 0.4157951 FAT4 FAT tumor suppressor homolog 4 (Drosophila) 293 +/- 14 306 +/- 33 708 +/- 23 485 +/- 48 0.000342 0.734089 -0.005929 0.523441 0.1234583 FBF1 Fas (TNFRSF6) binding factor 1 301 +/- 22 263 +/- 19 385 +/- 40 329 +/- 21 0.001402 -0.218039 -0.255397 0.4085579 0.5000467 FBS1 fibrosin 1 289 +/- 27 327 +/- 24 478 +/- 34 434 +/- 31 0.000393 0.3114305 -0.362316 0.09211 0.8376005 FBXL14 F-box and leucine-rich repeat protein 14 1117 +/- 114 749 +/- 52 1272 +/- 40 979 +/- 38 0.01286 -0.022121 -0.000484 0.07579 0.0310178 FBXL16 F-box and leucine-rich repeat protein 16 16392 +/- 1271 14554 +/- 732 18139 +/- 806 17755 +/- 602 0.011005 -0.245562 -0.711925 0.0892318 0.6411969 FBXL22 F-box and leucine-rich repeat protein 22 361 +/- 49 489 +/- 61 492 +/- 40 573 +/- 39 0.004069 0.1327113 0.181173 0.5323009 0.48109 FBXL5 F-box and leucine-rich repeat protein 5 281 +/- 17 233 +/- 9 307 +/- 13 264 +/- 18 0.037003 -0.036268 -0.084253 0.1025076 0.2376194 FBXL8 F-box and leucine-rich repeat protein 8 206 +/- 19 215 +/- 15 261 +/- 19 254 +/- 18 0.011465 0.7200951 -0.794115 0.7535524 0.4887186

S2-genes differentially expressed across region.xls Page 9 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI FBXO11 F-box protein 11 186 +/- 11 190 +/- 7 236 +/- 5 209 +/- 13 0.00503 0.7763734 -0.112843 0.5277932 0.2062247 FBXO16 F-box protein 16 189 +/- 18 150 +/- 6 242 +/- 23 186 +/- 15 0.022549 -0.091117 -0.076947 0.2163744 0.0881347 FBXO17 F-box protein 17 244 +/- 20 351 +/- 30 356 +/- 58 418 +/- 44 0.032456 0.0166647 0.414235 0.1175588 0.2090408 FBXO21 F-box protein 21 1991 +/- 126 2086 +/- 145 2348 +/- 61 2519 +/- 105 0.000666 0.6300796 0.205681 0.8915782 0.7800935 FBXO22 F-box protein 22 180 +/- 15 116 +/- 11 204 +/- 17 146 +/- 19 0.02693 -0.007922 -0.047841 0.0438841 0.0742148 FBXO27 F-box protein 27 212 +/- 14 233 +/- 26 348 +/- 27 305 +/- 10 0.002175 0.5098698 -0.191056 0.6237129 0.3757434 FCMD Fukuyama type congenital muscular dystrophy (fukutin) 472 +/- 35 530 +/- 22 633 +/- 45 626 +/- 19 6.03E-05 0.1943305 -0.899316 0.3609528 0.8782935 FDPS farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstran 1589 +/- 215 1840 +/- 90 2139 +/- 93 2184 +/- 100 0.003061 0.3174014 0.749522 0.3701 0.9397995 FEM1C fem-1 homolog c (C.elegans) 612 +/- 43 626 +/- 59 1022 +/- 64 954 +/- 49 3.42E-07 0.8582392 -0.421968 0.9831552 0.4749837 FEZ2 fasciculation and elongation protein zeta 2 (zygin II) 1737 +/- 241 1573 +/- 117 2033 +/- 66 1982 +/- 96 0.02175 -0.560788 -0.673907 0.4311277 0.829374 FFAR1 free fatty acid receptor 1 207 +/- 12 189 +/- 7 233 +/- 9 225 +/- 11 0.014977 -0.245908 -0.575869 0.2867314 0.7268566 FGF14 fibroblast growth factor 14 412 +/- 60 310 +/- 56 536 +/- 10 473 +/- 30 0.001987 -0.246025 -0.104421 0.1099533 0.0242961 FGFR1OP FGFR1 oncogene partner 74 +/- 12 115 +/- 24 88 +/- 12 154 +/- 27 0.001951 0.173336 0.072678 0.0989278 0.0449516 FGFR3 fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) 423 +/- 43 492 +/- 67 667 +/- 47 764 +/- 48 0.000629 0.40945 0.181361 0.93586 0.8557974 FHL1 four and a half LIM domains 1 4434 +/- 296 3835 +/- 494 6047 +/- 320 5973 +/- 158 0.000198 -0.328015 -0.841396 0.2901799 0.7031929 FKBP8 FK506 binding protein 8, 38kDa 3108 +/- 68 2961 +/- 65 3952 +/- 187 3766 +/- 47 3.96E-06 -0.152896 -0.375424 0.0347444 0.7967463 FKRP fukutin related protein 1114 +/- 40 971 +/- 30 1164 +/- 29 1050 +/- 21 0.003275 -0.018583 -0.010989 0.0373924 0.1118994 FKSG2 apoptosis inhibitor 29339 +/- 1284 28372 +/- 715 30087 +/- 1812 33819 +/- 1043 0.039645 -0.529781 0.113107 0.2781718 0.4307543 FLJ44635 TPT1-like protein 1333 +/- 348 1462 +/- 245 2543 +/- 219 2505 +/- 175 0.003356 0.7685697 -0.89449 0.7290715 0.9303296 FLJ46385 FLJ46385 protein 191 +/- 14 173 +/- 15 254 +/- 16 224 +/- 23 0.000322 -0.415263 -0.31024 0.6144274 0.2681464 FLOT2 flotillin 2 1471 +/- 66 1362 +/- 76 1778 +/- 32 1705 +/- 72 2.11E-05 -0.306641 -0.391453 0.090366 0.0994665 FLRT3 fibronectin leucine rich transmembrane protein 3 419 +/- 78 293 +/- 23 1012 +/- 121 991 +/- 55 2.68E-05 -0.17456 -0.875918 0.4273151 0.7927669 FMN2 formin 2 1208 +/- 69 1285 +/- 50 1543 +/- 63 1483 +/- 92 0.000164 0.3917429 -0.606964 0.2662723 0.8023059 FMNL2 Formin-like 2 255 +/- 17 315 +/- 26 332 +/- 35 355 +/- 27 0.048316 0.089932 0.603152 0.3237956 0.7885349 FN3KRP fructosamine-3-kinase-related protein 2327 +/- 186 2932 +/- 222 2878 +/- 211 3527 +/- 36 0.006278 0.0643649 0.027114 0.2476558 0.0954689 FNDC3A fibronectin type III domain containing 3A 1222 +/- 87 1485 +/- 91 1606 +/- 69 1768 +/- 140 0.002547 0.0639021 0.340913 0.0070505 0.1317991 FOSB FBJ murine osteosarcoma viral oncogene homolog B 198 +/- 18 177 +/- 19 241 +/- 17 205 +/- 15 0.020095 -0.451011 -0.133046 0.2796759 0.2405775 FOXG1B forkhead box G1B 826 +/- 115 654 +/- 50 1306 +/- 91 1218 +/- 89 6.11E-05 -0.212258 -0.509875 0.0922756 0.3043829 FOXO1A forkhead box O1A (rhabdomyosarcoma) 720 +/- 44 1108 +/- 81 1000 +/- 116 1225 +/- 114 0.046704 0.0031203 0.199946 0.0662699 0.9658058 FREQ frequenin homolog (Drosophila) 6907 +/- 692 7729 +/- 846 8284 +/- 516 9850 +/- 567 0.019921 0.4703367 0.072857 0.9888769 0.2084912 FRMD6 FERM domain containing 6 525 +/- 56 619 +/- 76 674 +/- 46 801 +/- 55 0.032384 0.3435957 0.111662 0.7417658 0.1729376 FRMPD3 FERM and PDZ domain containing 3 272 +/- 14 234 +/- 12 489 +/- 36 416 +/- 20 5.84E-06 -0.069503 -0.11258 0.1358001 0.2627123 FRZB frizzled-related protein 1424 +/- 211 1158 +/- 155 1741 +/- 146 1961 +/- 70 0.003272 -0.336448 0.216872 0.1586214 0.3399969 FST follistatin 144 +/- 19 122 +/- 15 243 +/- 50 155 +/- 19 0.02443 -0.390855 -0.147771 0.6861016 0.2177977 FXYD2 FXYD domain containing ion transport regulator 2 277 +/- 35 363 +/- 36 397 +/- 24 387 +/- 49 0.034664 0.1194221 -0.860187 0.2084073 0.5366977 FXYD7 FXYD domain containing ion transport regulator 7 3435 +/- 178 2940 +/- 109 4078 +/- 119 3624 +/- 147 0.000845 -0.044077 -0.042429 0.0048882 0.4833482 FYN FYN oncogene related to SRC, FGR, YES 243 +/- 42 94 +/- 25 298 +/- 27 175 +/- 27 0.019308 -0.014817 -0.010902 0.0085926 0.0075934 FYTTD1 forty-two-three domain containing 1 2121 +/- 87 2284 +/- 62 2708 +/- 97 2791 +/- 90 5.17E-07 0.1629674 0.548577 0.067963 0.395422 FZD2 frizzled homolog 2 (Drosophila) 334 +/- 25 288 +/- 17 413 +/- 18 436 +/- 26 0.000698 -0.170664 0.491132 0.0405848 0.7298265 FZD3 frizzled homolog 3 (Drosophila) 131 +/- 15 121 +/- 13 197 +/- 17 207 +/- 24 0.000222 -0.617122 0.726419 0.5251586 0.8708405 FZD7 frizzled homolog 7 (Drosophila) 1370 +/- 195 1399 +/- 180 2077 +/- 204 2255 +/- 352 0.005963 0.9128107 0.674675 0.8398471 0.1989453 G3BP2 Ras-GTPase activating protein SH3 domain-binding protein 2 1789 +/- 231 1551 +/- 163 2121 +/- 201 2288 +/- 214 0.023349 -0.420581 0.583686 0.2960857 0.9346932 G6PC3 glucose 6 phosphatase, catalytic, 3 461 +/- 24 427 +/- 16 500 +/- 22 491 +/- 18 0.031307 -0.281473 -0.752934 0.4959696 0.7923472 GABARAP GABA(A) receptor-associated protein like 1 9847 +/- 283 9124 +/- 693 11429 +/- 285 10912 +/- 593 0.000133 -0.368485 -0.462866 0.1495443 0.5761301 GABBR2 gamma-aminobutyric acid (GABA) B receptor, 2 1834 +/- 197 1551 +/- 193 2778 +/- 78 2396 +/- 114 0.000164 -0.329709 -0.026833 0.295949 0.324515

S2-genes differentially expressed across region.xls Page 10 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI GABRA2 gamma-aminobutyric acid (GABA) A receptor, alpha 2 57 +/- 8 75 +/- 13 170 +/- 57 157 +/- 30 0.005803 0.2646191 -0.846925 0.8127874 0.5829782 GABRA4 gamma-aminobutyric acid (GABA) A receptor, alpha 4 183 +/- 26 187 +/- 33 257 +/- 19 281 +/- 28 0.009741 0.9302957 0.509349 0.8329809 0.7571369 GABRA5 gamma-aminobutyric acid (GABA) A receptor, alpha 5 838 +/- 204 422 +/- 28 1101 +/- 147 1162 +/- 133 0.022414 -0.097817 0.767828 0.1039505 0.9880073 GABRD gamma-aminobutyric acid (GABA) A receptor, delta 701 +/- 68 649 +/- 83 1003 +/- 85 887 +/- 75 0.026986 -0.636314 -0.333611 0.4160224 0.622816 GABRG1 gamma-aminobutyric acid (GABA) A receptor, gamma 1 342 +/- 56 432 +/- 60 483 +/- 43 607 +/- 92 0.007933 0.3039362 0.269559 0.5849873 0.7187393 GABRG2 gamma-aminobutyric acid (GABA) A receptor, gamma 2 1039 +/- 164 880 +/- 168 1298 +/- 128 1363 +/- 117 0.009642 -0.513831 0.716639 0.2837804 0.9899453 GABRG3 gamma-aminobutyric acid (GABA) A receptor, gamma 3 83 +/- 11 74 +/- 14 144 +/- 6 100 +/- 6 0.000909 -0.596888 -0.000761 0.4350859 0.0006531 GALNT13 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13 ( 136 +/- 13 176 +/- 20 297 +/- 43 257 +/- 9 0.001412 0.1351323 -0.396693 0.1590541 0.1878207 GAMT guanidinoacetate N-methyltransferase 1022 +/- 43 1199 +/- 29 1147 +/- 61 1359 +/- 156 0.043166 0.009401 0.261073 0.0097846 0.1781006 GAS2L1 growth arrest-specific 2 like 1 943 +/- 45 848 +/- 37 1121 +/- 40 1151 +/- 41 0.000767 -0.13517 0.612697 0.0671882 0.3502762 GCH1 GTP cyclohydrolase 1 (dopa-responsive dystonia) 291 +/- 49 249 +/- 25 329 +/- 12 310 +/- 6 0.04428 -0.470068 -0.19348 0.6914637 0.5471291 GCLM glutamate-cysteine ligase, modifier subunit 771 +/- 36 943 +/- 26 851 +/- 27 1048 +/- 44 0.014231 0.0036647 0.007251 0.0229726 0.0130043 GCSH glycine cleavage system protein H (aminomethyl carrier) 1237 +/- 28 1190 +/- 53 1393 +/- 61 1377 +/- 35 0.004168 -0.45238 -0.822031 0.9678108 0.6651284 GDAP1 Ganglioside-induced differentiation-associated protein 1 919 +/- 22 758 +/- 116 990 +/- 105 1037 +/- 86 0.045305 -0.225724 0.738091 0.1511409 0.9772839 GDF1 growth differentiation factor 1 3123 +/- 224 3123 +/- 191 4092 +/- 159 4433 +/- 137 3.52E-05 -0.999455 0.140238 0.8322473 0.3915141 GEFT RAC/CDC42 exchange factor 4463 +/- 807 3672 +/- 634 5521 +/- 340 5416 +/- 232 0.007289 -0.459803 -0.804137 0.5017979 0.9708475 GEMIN4 gem (nuclear organelle) associated protein 4 297 +/- 12 327 +/- 28 385 +/- 46 421 +/- 32 0.016723 0.3698105 0.531421 0.5633264 0.4472374 GFM1 G elongation factor, mitochondrial 1 175 +/- 15 233 +/- 22 216 +/- 6 265 +/- 11 0.043576 0.0637293 0.006752 0.1726557 0.0006379 GFOD1 glucose-fructose oxidoreductase domain containing 1 75 +/- 14 98 +/- 8 118 +/- 8 127 +/- 16 0.005827 0.1802826 0.649305 0.2751452 0.834584 GGA2 golgi associated, gamma adaptin ear containing, ARF binding protein 2 287 +/- 13 339 +/- 28 373 +/- 22 349 +/- 15 0.013331 0.1346189 -0.398993 0.6237514 0.1638804 GGTL3 gamma-glutamyltransferase-like 3 2529 +/- 133 2114 +/- 121 2837 +/- 156 2630 +/- 103 0.001707 -0.044208 -0.298117 0.1027986 0.4930832 GLI2 GLI-Kruppel family member GLI2 129 +/- 11 140 +/- 14 176 +/- 10 134 +/- 12 0.036059 0.529673 -0.024784 0.3778247 0.0012399 GLI4 GLI-Kruppel family member GLI4 125 +/- 9 133 +/- 5 151 +/- 11 174 +/- 16 0.016417 0.4595862 0.274318 0.6359208 0.5492366 GLIS3 GLIS family zinc finger 3 388 +/- 37 480 +/- 71 558 +/- 66 677 +/- 59 0.015482 0.2833095 0.214882 0.5336861 0.3416032 GLRX glutaredoxin (thioltransferase) 834 +/- 71 849 +/- 75 1050 +/- 90 1074 +/- 86 4.62E-05 0.8871256 0.852071 0.7512997 0.5864873 GLS2 glutaminase 2 (liver, mitochondrial) 82 +/- 12 93 +/- 9 102 +/- 9 138 +/- 21 0.030768 0.4757278 0.179944 0.821633 0.4060063 GLT28D1 glycosyltransferase 28 domain containing 1 327 +/- 25 277 +/- 25 366 +/- 8 330 +/- 30 0.016684 -0.194319 -0.30991 0.6877399 0.9688704 GMPR guanosine monophosphate reductase 374 +/- 38 297 +/- 19 429 +/- 26 379 +/- 7 0.004685 -0.115215 -0.108907 0.3935775 0.2870423 GNAL guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfact 140 +/- 19 156 +/- 36 298 +/- 25 321 +/- 39 0.000523 0.7077585 0.625625 0.8691083 0.6103434 GNAS GNAS complex locus 3449 +/- 199 3168 +/- 250 4315 +/- 161 3991 +/- 231 1.65E-05 -0.401516 -0.284916 0.2674715 0.1274547 GNB1 Guanine nucleotide binding protein (G protein), beta polypeptide 1 353 +/- 46 428 +/- 41 522 +/- 40 546 +/- 40 0.000334 0.254155 0.676345 0.7893997 0.6535021 GNB3 guanine nucleotide binding protein (G protein), beta polypeptide 3 421 +/- 47 444 +/- 41 494 +/- 49 505 +/- 47 0.027355 0.7241183 0.879865 0.869043 0.888017 GNG2 guanine nucleotide binding protein (G protein), gamma 2 7405 +/- 371 5485 +/- 393 7908 +/- 317 6914 +/- 174 0.032401 -0.005281 -0.026402 0.0148569 0.0383324 GNG8 guanine nucleotide binding protein (G protein), gamma 8 409 +/- 52 397 +/- 57 518 +/- 45 467 +/- 27 0.007122 -0.882171 -0.353694 0.4651831 0.3743873 GNL1 guanine nucleotide binding protein-like 1 904 +/- 30 837 +/- 29 948 +/- 17 954 +/- 43 0.008019 -0.141093 0.892055 0.050439 0.5760857 GNL3 guanine nucleotide binding protein-like 3 (nucleolar) 1815 +/- 92 1779 +/- 43 1965 +/- 44 1955 +/- 52 0.019684 -0.733802 -0.891571 0.5039377 0.6281171 GNPDA2 glucosamine-6-phosphate deaminase 2 534 +/- 61 533 +/- 53 619 +/- 33 711 +/- 36 0.002453 -0.987729 0.094687 0.9060291 0.0323168 GNPNAT1 glucosamine-phosphate N-acetyltransferase 1 238 +/- 18 232 +/- 12 300 +/- 12 272 +/- 14 0.00129 -0.789861 -0.149359 0.5986641 0.2879633 GNPTAB N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits 215 +/- 29 180 +/- 19 266 +/- 35 230 +/- 23 0.025482 -0.344188 -0.415843 0.4108043 0.3759269 GOLGA2 golgi autoantigen, golgin subfamily a, 2 80 +/- 6 112 +/- 6 118 +/- 19 140 +/- 9 0.009563 0.004714 0.328757 0.0191052 0.5915585 GOLGA3 golgi autoantigen, golgin subfamily a, 3 381 +/- 32 421 +/- 43 537 +/- 24 537 +/- 52 0.003461 0.4731609 0.993657 0.6605646 0.8496925 GOLGA4 golgi autoantigen, golgin subfamily a, 4 158 +/- 20 223 +/- 19 236 +/- 13 257 +/- 20 0.001219 0.0401902 0.414473 0.0194827 0.1549389 GOLGA8A golgi autoantigen, golgin subfamily a, 8A 394 +/- 25 529 +/- 94 697 +/- 69 702 +/- 40 0.012805 0.2181383 0.947143 0.3422535 0.5193582 GOPC golgi associated PDZ and coiled-coil motif containing 462 +/- 34 622 +/- 43 560 +/- 32 730 +/- 26 0.005407 0.0158442 0.002573 0.0673328 0.0086109 GPC4 glypican 4 206 +/- 17 250 +/- 35 346 +/- 28 436 +/- 14 0.000173 0.2887487 0.023212 0.6553604 0.2101967

S2-genes differentially expressed across region.xls Page 11 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI GPD1 glycerol-3-phosphate dehydrogenase 1 (soluble) 451 +/- 61 597 +/- 65 607 +/- 50 773 +/- 69 0.045866 0.1314136 0.089405 0.423724 0.298508 GPD2 glycerol-3-phosphate dehydrogenase 2 (mitochondrial) 2261 +/- 127 1979 +/- 148 2578 +/- 83 2297 +/- 121 0.01089 -0.178976 -0.095333 0.3208829 0.575921 GPM6B glycoprotein M6B 5149 +/- 133 6879 +/- 254 6195 +/- 386 7340 +/- 323 0.026838 0.0003929 0.049273 0.0074918 0.472334 GPR113 G protein-coupled receptor 113 563 +/- 48 608 +/- 39 717 +/- 33 746 +/- 36 0.000884 0.4796388 0.574288 0.3806208 0.4497928 GPR135 G protein-coupled receptor 135 154 +/- 8 126 +/- 19 203 +/- 9 163 +/- 8 0.000126 -0.21993 -0.012068 0.5096649 0.0210926 GPR137 G protein-coupled receptor 137 729 +/- 83 643 +/- 52 973 +/- 57 1145 +/- 56 0.000209 -0.400288 0.058957 0.194919 0.3349041 GPR155 G protein-coupled receptor 155 3986 +/- 374 3280 +/- 279 5230 +/- 184 4681 +/- 151 3.3E-05 -0.164043 -0.046513 0.3330083 0.2508683 GPR172A G protein-coupled receptor 172A 286 +/- 15 326 +/- 19 338 +/- 12 328 +/- 11 0.040254 0.1274381 -0.562292 0.3696797 0.2480862 GPR180 G protein-coupled receptor 180 132 +/- 7 151 +/- 16 204 +/- 21 167 +/- 18 0.006745 0.3204351 -0.205347 0.2462715 0.2568266 GPR27 G protein-coupled receptor 27 529 +/- 62 481 +/- 36 639 +/- 57 570 +/- 34 0.042753 -0.520105 -0.330107 0.2696002 0.297231 GPR85 G protein-coupled receptor 85 1693 +/- 269 1343 +/- 234 2174 +/- 179 2095 +/- 40 0.002029 -0.349516 -0.680881 0.2561828 0.6094984 GPT glutamic-pyruvate transaminase (alanine aminotransferase) 486 +/- 30 518 +/- 24 544 +/- 23 561 +/- 13 0.021849 0.4113017 0.54496 0.6801934 0.7675988 GRB2 growth factor receptor-bound protein 2 1517 +/- 146 1282 +/- 44 1734 +/- 63 1619 +/- 38 0.011426 -0.175326 -0.154069 0.2972443 0.4572524 GREB1 GREB1 protein 96 +/- 10 88 +/- 4 144 +/- 12 135 +/- 16 0.000699 -0.472592 -0.682844 0.7973792 0.7831327 GRIA1 Glutamate receptor, ionotropic, AMPA 1 3467 +/- 436 2792 +/- 443 4126 +/- 258 3711 +/- 227 0.003329 -0.302831 -0.257704 0.2975945 0.1636895 GRIK5 glutamate receptor, ionotropic, kainate 5 277 +/- 19 255 +/- 19 562 +/- 22 498 +/- 49 8.77E-06 -0.434699 -0.286314 0.4054351 0.7718675 GRIN1 glutamate receptor, ionotropic, N-methyl D-aspartate 1 3870 +/- 384 3282 +/- 267 4398 +/- 321 3934 +/- 220 0.002578 -0.239918 -0.264496 0.1362697 0.1880672 GRINA glutamate receptor, ionotropic, N-methyl D-asparate-associated protein 1 (glutamate bind 5549 +/- 298 4625 +/- 234 6955 +/- 300 6870 +/- 234 2.45E-05 -0.036044 -0.828973 0.0232317 0.6928194 GRK4 G protein-coupled receptor kinase 4 143 +/- 17 209 +/- 46 181 +/- 22 264 +/- 67 0.004132 0.2320659 0.295227 0.0623202 0.0840736 GRK5 G protein-coupled receptor kinase 5 137 +/- 12 120 +/- 4 241 +/- 16 198 +/- 21 4.62E-05 -0.252808 -0.134346 0.2761808 0.2275984 GRLF1 glucocorticoid receptor DNA binding factor 1 2246 +/- 57 1916 +/- 112 2476 +/- 123 2257 +/- 73 0.029725 -0.031675 -0.162091 0.0059261 0.5659028 GRM1 glutamate receptor, metabotropic 1 307 +/- 35 313 +/- 56 533 +/- 65 555 +/- 37 0.001219 0.9373081 0.773595 0.8368322 0.8886467 GRM2 glutamate receptor, metabotropic 2 418 +/- 73 480 +/- 42 730 +/- 37 724 +/- 63 0.001939 0.4840915 -0.937246 0.5220645 0.5923801 GRP gastrin-releasing peptide 586 +/- 219 844 +/- 288 2051 +/- 268 2392 +/- 358 0.001535 0.4937027 0.468801 0.6502949 0.6941366 GRPEL2 GrpE-like 2, mitochondrial (E. coli) 607 +/- 25 650 +/- 49 682 +/- 25 684 +/- 39 0.044846 0.4535761 0.969181 0.8714972 0.2324075 GSTM4 glutathione S-transferase M4 298 +/- 31 363 +/- 47 423 +/- 21 462 +/- 29 0.002276 0.2820748 0.314813 0.4815968 0.2712704 GSTM5 glutathione S-transferase M5 2800 +/- 328 2758 +/- 252 3679 +/- 430 3715 +/- 211 0.002895 -0.921256 0.943222 0.9192678 0.4723403 GTDC1 glycosyltransferase-like domain containing 1 360 +/- 16 422 +/- 48 436 +/- 42 481 +/- 27 0.01014 0.2578888 0.387105 0.2335922 0.3734054 GUCY1A2 guanylate cyclase 1, soluble, alpha 2 96 +/- 13 85 +/- 10 150 +/- 27 154 +/- 17 0.014184 -0.501046 0.911147 0.6043195 0.981656 GUCY1A3 Guanylate cyclase 1, soluble, alpha 3 1920 +/- 323 2521 +/- 220 2846 +/- 165 3163 +/- 421 0.01302 0.1585896 0.512546 0.1393403 0.3832425 H1FX H1 histone family, member X 1737 +/- 192 1668 +/- 134 2282 +/- 156 2483 +/- 184 0.001001 -0.776865 0.42888 0.7052893 0.409936 HARS histidyl-tRNA synthetase 1394 +/- 62 1252 +/- 62 1493 +/- 15 1500 +/- 30 0.005996 -0.136764 0.838428 0.152974 0.2899605 HCA112 hepatocellular carcinoma-associated antigen 112 690 +/- 60 647 +/- 42 806 +/- 33 740 +/- 17 0.015848 -0.573373 -0.115838 0.7563873 0.3084458 HCCS holocytochrome c synthase (cytochrome c heme-lyase) 1410 +/- 87 1271 +/- 59 1539 +/- 74 1469 +/- 44 0.000618 -0.220366 -0.438849 0.1417296 0.2996703 HCFC1 host cell factor C1 (VP16-accessory protein) 314 +/- 21 281 +/- 21 373 +/- 15 336 +/- 26 0.001016 -0.297414 -0.250952 0.8516292 0.4392442 HDAC10 histone deacetylase 10 591 +/- 58 670 +/- 49 985 +/- 78 1051 +/- 80 0.000236 0.3280811 0.573331 0.8933866 0.7304392 HDAC2 histone deacetylase 2 1860 +/- 100 1904 +/- 97 2276 +/- 79 2208 +/- 82 0.002372 0.7567067 -0.568327 0.6282243 0.6304701 HDGFRP3 hepatoma-derived growth factor, related protein 3 4085 +/- 421 3281 +/- 207 5237 +/- 272 5467 +/- 363 0.000191 -0.128682 0.627277 0.048227 0.9714237 HDLBP high density lipoprotein binding protein (vigilin) 416 +/- 18 379 +/- 20 448 +/- 16 464 +/- 24 0.024441 -0.196271 0.611821 0.5856326 0.2957037 HEATR2 HEAT repeat containing 2 236 +/- 26 286 +/- 20 347 +/- 18 351 +/- 21 0.003897 0.165463 0.90079 0.2922576 0.8619665 HEATR3 HEAT repeat containing 3 584 +/- 59 612 +/- 30 808 +/- 50 658 +/- 71 0.015511 0.6834378 -0.123977 0.4237959 0.4240297 HEBP2 heme binding protein 2 125 +/- 34 114 +/- 13 177 +/- 11 138 +/- 14 0.020881 -0.769882 -0.07033 0.8551733 0.053405 HECTD1 HECT domain containing 1 154 +/- 14 131 +/- 13 204 +/- 13 162 +/- 11 0.009086 -0.238273 -0.041532 0.852479 0.2248975 HERC2P7 hect domain and RLD 2 pseudogene 7 170 +/- 14 126 +/- 21 225 +/- 17 160 +/- 19 0.014426 -0.114895 -0.033002 0.110836 0.0130911 HEXDC hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing 148 +/- 17 180 +/- 18 173 +/- 20 274 +/- 24 0.002521 0.2261819 0.011704 0.4469197 0.0067908

S2-genes differentially expressed across region.xls Page 12 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI HFE hemochromatosis 171 +/- 34 112 +/- 14 205 +/- 34 169 +/- 21 0.013946 -0.149714 -0.384548 0.1243031 0.098543 HIC2 hypermethylated in cancer 2 316 +/- 33 385 +/- 11 436 +/- 19 484 +/- 44 0.001918 0.0942498 0.356125 0.1175151 0.3392852 HISPPD2A Histidine acid phosphatase domain containing 2A 1213 +/- 73 1266 +/- 63 1466 +/- 60 1458 +/- 61 0.011616 0.5940609 -0.931676 0.9300534 0.8525424 HMGCR 3-hydroxy-3-methylglutaryl-Coenzyme A reductase 1890 +/- 172 1539 +/- 69 2130 +/- 95 1817 +/- 102 0.047367 -0.101924 -0.052681 0.0647459 0.186102 HMGCS1 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) 888 +/- 127 1088 +/- 135 1339 +/- 193 1624 +/- 217 0.005967 0.30577 0.352461 0.6309546 0.7187677 HMGCS2 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) 244 +/- 51 182 +/- 18 480 +/- 52 240 +/- 77 0.015867 -0.292636 -0.035266 0.5851959 0.0341019 HMGN4 high mobility group nucleosomal binding domain 4 674 +/- 26 748 +/- 34 777 +/- 29 836 +/- 55 0.009419 0.1181175 0.378199 0.0081088 0.1820736 HN1 hematological and neurological expressed 1 2135 +/- 127 1867 +/- 131 2279 +/- 72 2214 +/- 49 0.005547 -0.173159 -0.474472 0.1677082 0.3839684 HNMT histamine N-methyltransferase 396 +/- 32 235 +/- 22 494 +/- 59 338 +/- 17 0.006664 -0.002524 -0.044935 0.0213492 0.0723774 HNRPA3P1heterogeneous nuclear ribonucleoprotein A3 pseudogene 1 1370 +/- 59 1182 +/- 49 1527 +/- 55 1349 +/- 65 0.026639 -0.033981 -0.067248 0.1591375 0.2003319 HNRPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kD 1406 +/- 64 1586 +/- 51 1838 +/- 60 1825 +/- 128 0.000959 0.0542112 -0.927465 0.2100338 0.9676209 HNRPH1 heterogeneous nuclear ribonucleoprotein H1 (H) 235 +/- 32 224 +/- 31 278 +/- 35 343 +/- 48 0.009307 -0.813817 0.311559 0.7810284 0.3762832 HNRPH3 heterogeneous nuclear ribonucleoprotein H3 (2H9) 973 +/- 64 1240 +/- 83 1247 +/- 84 1322 +/- 104 0.019894 0.0303551 0.590177 0.0251709 0.9298828 HNRPL heterogeneous nuclear ribonucleoprotein L 5232 +/- 292 6250 +/- 170 6161 +/- 225 7259 +/- 144 0.000633 0.0167305 0.003234 0.1424168 0.0232842 HNRPLL heterogeneous nuclear ribonucleoprotein L-like 2146 +/- 110 2030 +/- 59 2632 +/- 129 2515 +/- 72 0.00017 -0.382238 -0.451629 0.6659535 0.6115848 HNRPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) 398 +/- 37 321 +/- 12 490 +/- 22 438 +/- 23 0.002062 -0.099309 -0.149069 0.164647 0.2954007 HNRPUL1 heterogeneous nuclear ribonucleoprotein U-like 1 1585 +/- 48 1735 +/- 65 2072 +/- 63 2094 +/- 52 2.07E-05 0.0951585 0.787684 0.0367925 0.4223104 HNRPUL2 heterogeneous nuclear ribonucleoprotein U-like 2 237 +/- 37 290 +/- 23 318 +/- 35 333 +/- 27 0.034099 0.2442639 0.742169 0.4194135 0.9459743 HOMER3 homer homolog 3 (Drosophila) 250 +/- 55 168 +/- 32 356 +/- 16 267 +/- 46 0.042918 -0.235754 -0.127127 0.3149873 0.5691435 HPCA hippocalcin 4525 +/- 1471 3518 +/- 972 6948 +/- 943 7510 +/- 790 0.001009 -0.582327 0.658268 0.524834 0.8374407 HPCAL1 hippocalcin-like 1 2659 +/- 438 3108 +/- 442 4291 +/- 447 3897 +/- 386 0.031443 0.486751 -0.522166 0.8306465 0.318843 HPS6 Hermansky-Pudlak syndrome 6 434 +/- 16 386 +/- 21 475 +/- 21 482 +/- 25 0.021706 -0.100081 0.836963 0.1500101 0.6898117 HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog 1817 +/- 77 1987 +/- 109 2042 +/- 85 2290 +/- 106 0.02028 0.235603 0.104562 0.657285 0.1823637 HS2ST1 heparan sulfate 2-O-sulfotransferase 1 360 +/- 44 287 +/- 18 416 +/- 11 365 +/- 23 0.038668 -0.170988 -0.092215 0.1631496 0.2742107 HSF1 heat shock transcription factor 1 560 +/- 17 589 +/- 8 660 +/- 38 628 +/- 44 0.028456 0.1619299 -0.598394 0.2298407 0.8881215 HSFX1 heat shock transcription factor family, X linked 1 176 +/- 12 194 +/- 11 228 +/- 7 212 +/- 15 0.010125 0.3121045 -0.36992 0.6879921 0.3948915 HSPBAP1 HSPB (heat shock 27kDa) associated protein 1 348 +/- 61 277 +/- 18 428 +/- 62 447 +/- 30 0.001334 -0.307183 0.801409 0.7873278 0.4533114 HTATSF1 HIV-1 Tat specific factor 1 303 +/- 38 331 +/- 35 382 +/- 17 411 +/- 24 0.034487 0.5984194 0.359528 0.7409079 0.2627508 HTF9C HpaII tiny fragments locus 9C 237 +/- 18 254 +/- 15 332 +/- 19 284 +/- 19 0.005588 0.5139139 -0.111411 0.4184348 0.3975385 HTR1E 5-hydroxytryptamine (serotonin) receptor 1E 240 +/- 27 232 +/- 25 373 +/- 34 364 +/- 19 0.000181 -0.830667 -0.816784 0.8928121 0.6659734 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 166 +/- 21 103 +/- 13 182 +/- 25 126 +/- 12 0.045284 -0.03231 -0.078401 0.2517837 0.1153546 HTR6 5-hydroxytryptamine (serotonin) receptor 6 215 +/- 16 173 +/- 20 309 +/- 33 301 +/- 16 9.04E-05 -0.135812 -0.827321 0.3142413 0.9396733 HUWE1 HECT, UBA and WWE domain containing 1 814 +/- 59 736 +/- 76 1046 +/- 70 945 +/- 85 0.017673 -0.435488 -0.387623 0.4131299 0.6272215 HYI hydroxypyruvate isomerase homolog (E. coli) 463 +/- 46 521 +/- 42 537 +/- 51 594 +/- 37 0.01668 0.3744449 0.391382 0.4585689 0.5332631 IBRDC3 IBR domain containing 3 179 +/- 43 292 +/- 22 217 +/- 41 344 +/- 26 0.004416 0.0476291 0.029838 0.2191918 0.2995504 ICAM5 intercellular adhesion molecule 5, telencephalin 1523 +/- 281 1420 +/- 243 1957 +/- 122 2241 +/- 112 0.007053 -0.785652 0.121609 0.6239313 0.3475594 ID2 inhibitor of DNA binding 2, dominant negative helix-loop-helix protein 2280 +/- 212 2637 +/- 202 3203 +/- 248 2865 +/- 90 0.028319 0.2513884 -0.245195 0.3611387 0.4927106 IDH3A isocitrate dehydrogenase 3 (NAD+) alpha 1935 +/- 237 1452 +/- 86 2076 +/- 200 2187 +/- 115 0.037656 -0.101217 0.645302 0.0840321 0.8426569 IDS iduronate 2-sulfatase (Hunter syndrome) 10394 +/- 724 9112 +/- 735 15736 +/- 599 15812 +/- 752 1.85E-05 -0.242618 0.938793 0.0762014 0.7956363 IER5 immediate early response 5 755 +/- 51 814 +/- 44 1018 +/- 84 905 +/- 41 0.012306 0.3973124 -0.267984 0.7081438 0.4821491 IFT140 intraflagellar transport 140 homolog (Chlamydomonas) 212 +/- 12 263 +/- 17 244 +/- 23 276 +/- 16 0.045038 0.0372744 0.272873 0.071048 0.3425192 IFT52 intraflagellar transport 52 homolog (Chlamydomonas) 799 +/- 54 918 +/- 33 929 +/- 63 1021 +/- 27 0.003244 0.0941256 0.221829 0.1459342 0.1899747 IGF1 insulin-like growth factor 1 (somatomedin C) 111 +/- 20 131 +/- 23 173 +/- 16 154 +/- 11 0.009665 0.5345284 -0.373254 0.6335202 0.6232785 IGFBP2 insulin-like growth factor binding protein 2, 36kDa 370 +/- 44 326 +/- 37 652 +/- 87 579 +/- 101 0.000872 -0.485188 -0.598233 0.1163711 0.4198447 IGHMBP2 immunoglobulin mu binding protein 2 554 +/- 41 550 +/- 55 617 +/- 24 699 +/- 66 0.014741 -0.963606 0.295088 0.7070517 0.3533595

S2-genes differentially expressed across region.xls Page 13 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI IGSF4D immunoglobulin superfamily, member 4D 1940 +/- 206 1782 +/- 134 2685 +/- 208 2627 +/- 75 6.8E-06 -0.538536 -0.800081 0.5051144 0.9677798 IGSF8 immunoglobulin superfamily, member 8 4894 +/- 541 4812 +/- 361 6767 +/- 442 6580 +/- 191 0.000917 -0.901578 -0.710392 0.636277 0.7357781 IL17D Interleukin 17D 1606 +/- 78 1561 +/- 74 1700 +/- 81 1768 +/- 30 0.012473 -0.682848 0.454498 0.2227996 0.5948912 IL27RA interleukin 27 receptor, alpha 828 +/- 63 715 +/- 55 949 +/- 34 878 +/- 37 0.003618 -0.207187 -0.19514 0.3098327 0.4235523 INADL InaD-like (Drosophila) 87 +/- 10 135 +/- 10 146 +/- 6 143 +/- 14 0.010416 0.0084226 -0.845084 0.0070272 0.4992556 ING1 inhibitor of growth family, member 1 186 +/- 13 186 +/- 6 282 +/- 13 272 +/- 18 1.41E-06 -0.971879 -0.680355 0.6826541 0.8893313 ING5 inhibitor of growth family, member 5 476 +/- 29 580 +/- 43 552 +/- 25 617 +/- 39 0.000235 0.0763868 0.210031 0.1598318 0.7769837 INPP5B inositol polyphosphate-5-phosphatase, 75kDa 713 +/- 76 853 +/- 19 911 +/- 51 953 +/- 52 0.020455 0.1261006 0.577468 0.187619 0.9563544 INSIG2 insulin induced gene 2 1452 +/- 70 1343 +/- 45 1520 +/- 41 1523 +/- 36 0.04289 -0.223566 0.956665 0.1441873 0.5775221 INVS inversin 194 +/- 23 211 +/- 24 213 +/- 20 234 +/- 32 0.020893 0.6114824 0.594496 0.7407488 0.7511405 IPO9 importin 9 1211 +/- 95 1365 +/- 81 1369 +/- 47 1573 +/- 68 0.010492 0.2437822 0.042275 0.2746075 0.0033788 IQCA IQ motif containing with AAA domain 229 +/- 18 273 +/- 33 294 +/- 35 338 +/- 43 0.003202 0.2729979 0.449717 0.2129285 0.1890866 IQCE IQ motif containing E 730 +/- 62 780 +/- 65 1107 +/- 59 1179 +/- 64 6.25E-06 0.5938427 0.429064 0.6022065 0.0956478 IRF2 Interferon regulatory factor 2 107 +/- 6 144 +/- 17 144 +/- 15 154 +/- 14 0.024761 0.0785215 0.624112 0.0237249 0.3975184 IRF2BP1 interferon regulatory factor 2 binding protein 1 670 +/- 55 598 +/- 44 766 +/- 42 707 +/- 50 1.33E-05 -0.329302 -0.386847 0.9177444 0.6375269 ISLR2 immunoglobulin superfamily containing leucine-rich repeat 2 424 +/- 36 459 +/- 44 763 +/- 160 734 +/- 58 0.00695 0.5466159 -0.872166 0.9717311 0.8218768 ITGA7 integrin, alpha 7 389 +/- 76 464 +/- 92 862 +/- 112 749 +/- 58 0.007579 0.5465482 -0.398263 0.8453405 0.4909085 ITGB4 integrin, beta 4 433 +/- 101 626 +/- 98 854 +/- 90 1205 +/- 70 4.03E-05 0.1993225 0.013637 0.3119505 0.1281018 ITGBL1 Integrin, beta-like 1 (with EGF-like repeat domains) 93 +/- 24 53 +/- 9 161 +/- 21 143 +/- 14 2.34E-05 -0.162713 -0.498235 0.1259113 0.3718629 ITSN1 Intersectin 1 (SH3 domain protein) 111 +/- 11 94 +/- 11 157 +/- 18 110 +/- 31 0.015893 -0.29698 -0.228448 0.8433469 0.8498111 JAG2 jagged 2 536 +/- 24 626 +/- 44 662 +/- 54 716 +/- 70 0.015944 0.1127483 0.56334 0.1711277 0.2967106 JAM2 junctional adhesion molecule 2 636 +/- 61 627 +/- 41 711 +/- 45 744 +/- 37 0.011207 -0.895998 0.587077 0.5073111 0.8330516 JMJD1C jumonji domain containing 1C 827 +/- 66 909 +/- 72 1069 +/- 50 1184 +/- 57 2.87E-05 0.4223155 0.163598 0.285488 0.1940872 JMJD2B jumonji domain containing 2B 560 +/- 53 484 +/- 31 727 +/- 47 686 +/- 18 0.001229 -0.253275 -0.451231 0.3877165 0.8324024 JMJD3 jumonji domain containing 3 284 +/- 28 254 +/- 20 377 +/- 19 338 +/- 14 0.004005 -0.410618 -0.133085 0.3738156 0.1195429 JPH1 junctophilin 1 272 +/- 30 243 +/- 28 341 +/- 31 350 +/- 22 0.001426 -0.497456 0.815964 0.6778913 0.5875592 JUN v-jun sarcoma virus 17 oncogene homolog (avian) 700 +/- 55 634 +/- 49 1304 +/- 111 1155 +/- 39 5.94E-05 -0.391705 -0.250602 0.4950022 0.3608595 JUND jun D proto-oncogene 679 +/- 56 787 +/- 57 912 +/- 63 1118 +/- 106 0.00083 0.2023003 0.140334 0.3349942 0.0431503 KALRN Kalirin, RhoGEF kinase 475 +/- 54 556 +/- 48 860 +/- 121 921 +/- 109 0.00051 0.2851089 0.718312 0.555694 0.6324193 KBTBD11 kelch repeat and BTB (POZ) domain containing 11 5249 +/- 698 6336 +/- 653 6767 +/- 348 7712 +/- 454 0.010134 0.2820761 0.137638 0.3833872 0.2150897 KCNA3 potassium voltage-gated channel, shaker-related subfamily, member 3 1164 +/- 208 1231 +/- 268 2262 +/- 115 2064 +/- 121 0.001671 0.8489167 -0.266962 0.987146 0.3684191 KCNC4 potassium voltage-gated channel, Shaw-related subfamily, member 4 984 +/- 119 935 +/- 85 1328 +/- 106 1184 +/- 129 0.042686 -0.743626 -0.413179 0.3906643 0.3977448 KCNG3 potassium voltage-gated channel, subfamily G, member 3 523 +/- 91 366 +/- 66 679 +/- 39 608 +/- 33 0.008937 -0.195832 -0.198259 0.172145 0.5526645 KCNH4 potassium voltage-gated channel, subfamily H (eag-related), member 4 169 +/- 12 150 +/- 11 216 +/- 16 183 +/- 13 0.012511 -0.264364 -0.141993 0.3292707 0.1373193 KCNIP2 Kv channel interacting protein 2 431 +/- 85 376 +/- 106 985 +/- 40 993 +/- 45 3.01E-06 -0.691264 0.904062 0.5677863 0.6624142 KCNJ16 potassium inwardly-rectifying channel, subfamily J, member 16 132 +/- 23 102 +/- 14 178 +/- 23 116 +/- 14 0.010593 -0.296493 -0.050114 0.2193097 0.0426232 KCNK17 potassium channel, subfamily K, member 17 175 +/- 17 111 +/- 10 253 +/- 20 177 +/- 20 0.000433 -0.011002 -0.023816 0.0727271 0.0841288 KCNQ5 potassium voltage-gated channel, KQT-like subfamily, member 5 59 +/- 10 66 +/- 18 152 +/- 32 177 +/- 40 0.002529 0.7587449 0.642275 0.9241791 0.9925552 KCNS2 potassium voltage-gated channel, delayed-rectifier, subfamily S, member 2 234 +/- 46 284 +/- 60 424 +/- 6 400 +/- 19 0.009011 0.5311907 -0.284717 0.9951389 0.070614 KCNT2 Potassium channel, subfamily T, member 2 268 +/- 76 178 +/- 41 517 +/- 52 419 +/- 144 0.001616 -0.329348 -0.550496 0.4796351 0.4538349 KCTD12 potassium channel tetramerisation domain containing 12 4101 +/- 460 4864 +/- 238 5833 +/- 307 6138 +/- 213 3.49E-05 0.1807223 0.435947 0.4129087 0.9201584 KCTD17 potassium channel tetramerisation domain containing 17 288 +/- 31 189 +/- 16 389 +/- 31 381 +/- 41 0.003133 -0.022214 -0.885017 0.0242203 0.706816 KCTD4 potassium channel tetramerisation domain containing 4 2281 +/- 345 1961 +/- 245 2815 +/- 213 2898 +/- 46 0.001162 -0.46836 0.715737 0.6567367 0.6523038 KCTD6 potassium channel tetramerisation domain containing 6 2353 +/- 130 2478 +/- 383 5186 +/- 419 4859 +/- 318 2.02E-07 0.7700119 -0.550604 0.9370203 0.5564221 KCTD7 potassium channel tetramerisation domain containing 7 267 +/- 20 323 +/- 36 326 +/- 27 392 +/- 21 0.037404 0.2119376 0.082319 0.1270004 0.1744268

S2-genes differentially expressed across region.xls Page 14 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI KDELR1 KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1 1452 +/- 117 1410 +/- 52 2023 +/- 51 1999 +/- 50 1.58E-06 -0.755012 -0.73857 0.8268704 0.5485392 KDELR2 KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2 559 +/- 51 614 +/- 53 706 +/- 46 757 +/- 62 0.021324 0.4801087 0.521549 0.6808327 0.893134 KHDRBS3 KH domain containing, RNA binding, signal transduction associated 3 2499 +/- 180 2392 +/- 103 3016 +/- 147 2784 +/- 189 0.008025 -0.619665 -0.359977 0.6470696 0.7862015 KIAA1893 G protein-regulated inducer of neurite outgrowth 1 775 +/- 69 666 +/- 73 1435 +/- 125 1268 +/- 93 0.000114 -0.302767 -0.314141 0.4024895 0.560882 KIF21A kinesin family member 21A 3821 +/- 217 2757 +/- 229 4422 +/- 302 3119 +/- 95 0.000296 -0.007043 -0.00637 0.0635413 0.0334699 KIF3B kinesin family member 3B 1592 +/- 199 1106 +/- 96 1784 +/- 164 1327 +/- 44 0.033434 -0.062974 -0.037931 0.1085254 0.1408668 KIFAP3 Kinesin-associated protein 3 238 +/- 28 260 +/- 24 502 +/- 36 455 +/- 29 3.12E-05 0.5514032 -0.332079 0.8528891 0.4359631 KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog 4667 +/- 898 3619 +/- 752 5985 +/- 275 5401 +/- 655 0.010069 -0.392792 -0.445848 0.3987621 0.4003654 KLF13 Kruppel-like factor 13 1790 +/- 60 1158 +/- 151 2000 +/- 133 1313 +/- 196 0.021147 -0.006851 -0.02201 0.1488425 0.3862592 KLF16 Kruppel-like factor 16 1527 +/- 100 1253 +/- 111 1717 +/- 83 1565 +/- 66 0.004856 -0.098029 -0.187953 0.056007 0.2334464 KLF9 Kruppel-like factor 9 183 +/- 15 217 +/- 25 307 +/- 27 264 +/- 16 0.003894 0.2641485 -0.210261 0.4466555 0.3029169 KLHDC8A kelch domain containing 8A 686 +/- 105 674 +/- 74 1132 +/- 96 908 +/- 78 0.008728 -0.928761 -0.103777 0.867842 0.1019849 KLHDC8B kelch domain containing 8B 826 +/- 85 933 +/- 55 1066 +/- 46 1056 +/- 64 0.009987 0.3200842 -0.896994 0.5793904 0.1798474 KLHL12 kelch-like 12 (Drosophila) 496 +/- 56 456 +/- 40 622 +/- 52 642 +/- 58 0.002254 -0.576798 0.802859 0.3337456 0.7970282 KLHL2 kelch-like 2, Mayven (Drosophila) 4816 +/- 510 3890 +/- 688 7319 +/- 565 5661 +/- 464 0.000996 -0.306975 -0.049757 0.4627157 0.301802 KLHL23 kelch-like 23 (Drosophila) 1020 +/- 86 1240 +/- 95 1216 +/- 99 1392 +/- 89 0.002869 0.1166858 0.217507 0.5984007 0.6129454 KLHL25 kelch-like 25 (Drosophila) 287 +/- 20 348 +/- 24 415 +/- 25 411 +/- 18 0.003626 0.0766178 -0.889554 0.3562486 0.9184839 KLHL7 kelch-like 7 (Drosophila) 133 +/- 14 122 +/- 4 194 +/- 19 220 +/- 17 0.001843 -0.475304 0.327631 0.5814065 0.4098213 KLP1 K562 cell-derived leucine-zipper-like protein 1 2094 +/- 238 1827 +/- 124 2448 +/- 139 2466 +/- 103 0.001487 -0.350868 0.921092 0.444062 0.6980082 KPNA6 karyopherin alpha 6 (importin alpha 7) 351 +/- 17 399 +/- 37 432 +/- 15 435 +/- 25 0.036901 0.27178 0.930368 0.2579965 0.7139128 KTI12 KTI12 homolog, chromatin associated (S. cerevisiae) 730 +/- 40 633 +/- 40 843 +/- 23 827 +/- 43 6.22E-05 -0.115791 -0.747893 0.0482223 0.1717447 L1CAM L1 cell adhesion molecule 605 +/- 36 460 +/- 68 836 +/- 71 770 +/- 75 0.000367 -0.098096 -0.539336 0.1486527 0.881255 LARP1 La ribonucleoprotein domain family, member 1 2654 +/- 61 2210 +/- 101 2989 +/- 126 2762 +/- 90 0.000182 -0.005348 -0.178466 0.0044175 0.1765114 LARP4 La ribonucleoprotein domain family, member 4 166 +/- 10 226 +/- 28 218 +/- 15 281 +/- 67 0.007355 0.0850047 0.407276 0.5538886 0.9329248 LASS1 LAG1 longevity assurance homolog 1 (S. cerevisiae) 522 +/- 32 475 +/- 31 612 +/- 18 600 +/- 29 0.000454 -0.318118 -0.731634 0.5055776 0.2447506 LDOC1 leucine zipper, down-regulated in cancer 1 5604 +/- 497 5003 +/- 329 6003 +/- 186 5865 +/- 263 0.020232 -0.341571 -0.679001 0.1696446 0.3248242 LENG8 leukocyte receptor cluster (LRC) member 8 1847 +/- 278 1913 +/- 186 2578 +/- 194 2546 +/- 160 0.003858 0.8473801 -0.900734 0.8968818 0.8371419 LETM1 Leucine zipper-EF-hand containing transmembrane protein 1 657 +/- 89 493 +/- 44 787 +/- 66 695 +/- 75 0.015072 -0.141475 -0.383286 0.3173109 0.8063094 LGALS14 lectin, galactoside-binding, soluble, 14 188 +/- 23 237 +/- 28 217 +/- 25 242 +/- 37 0.040774 0.2050578 0.588314 0.2577323 0.9470156 LGI4 leucine-rich repeat LGI family, member 4 997 +/- 80 1142 +/- 70 1188 +/- 71 1200 +/- 77 0.001258 0.2035096 0.90965 0.192654 0.8466354 LGR5 leucine-rich repeat-containing G protein-coupled receptor 5 105 +/- 24 112 +/- 25 191 +/- 9 227 +/- 41 0.012207 0.827651 0.43584 0.8092794 0.3970343 LIMD2 LIM domain containing 2 662 +/- 37 561 +/- 70 1039 +/- 34 994 +/- 82 5.75E-05 -0.237391 -0.630843 0.3209015 0.4317537 LIMK1 LIM domain kinase 1 300 +/- 31 305 +/- 27 388 +/- 31 454 +/- 30 0.001386 0.9073882 0.161668 0.8432656 0.4218545 LIN7C lin-7 homolog C (C. elegans) 517 +/- 76 419 +/- 87 761 +/- 86 713 +/- 57 0.000577 -0.415881 -0.65744 0.299029 0.7459311 LIN9 lin-9 homolog (C. elegans) 100 +/- 19 109 +/- 20 180 +/- 21 131 +/- 5 0.009493 0.7354892 -0.06393 0.78466 0.0507246 LINS1 lines homolog 1 (Drosophila) 163 +/- 17 267 +/- 53 219 +/- 23 301 +/- 68 0.001456 0.1113764 0.306792 0.201907 0.4287942 LKAP limkain b1 149 +/- 26 143 +/- 34 216 +/- 33 226 +/- 18 0.038753 -0.901446 0.803337 0.9097234 0.7748115 LMBR1 limb region 1 homolog (mouse) 128 +/- 17 169 +/- 18 182 +/- 19 211 +/- 20 0.008148 0.1326831 0.321422 0.3524662 0.3874522 LMTK2 lemur tyrosine kinase 2 170 +/- 26 132 +/- 23 307 +/- 24 333 +/- 31 0.000104 -0.291227 0.519676 0.032858 0.4552069 LMTK3 lemur tyrosine kinase 3 2829 +/- 148 2865 +/- 137 5491 +/- 441 6358 +/- 451 1.2E-06 0.8617652 0.203198 0.8632938 0.6641366 LNPEP leucyl/cystinyl aminopeptidase 2120 +/- 127 2540 +/- 116 2687 +/- 122 2808 +/- 110 0.003585 0.0351572 0.478574 0.1309289 0.6568302 LOC51334 mesenchymal stem cell protein DSC54 149 +/- 18 123 +/- 12 220 +/- 9 173 +/- 19 0.004622 -0.261091 -0.074858 0.2001632 0.5641509 LOH11CR2loss of heterozygosity, 11, chromosomal region 2, gene A 89 +/- 17 88 +/- 3 310 +/- 27 344 +/- 32 5.99E-06 -0.989318 0.428491 0.813235 0.8124613 LONRF2 LON peptidase N-terminal domain and ring finger 2 5246 +/- 318 4952 +/- 358 6979 +/- 169 6923 +/- 170 9.94E-06 -0.553416 -0.819758 0.2949449 0.6316242 LOR loricrin 136 +/- 10 142 +/- 11 168 +/- 19 186 +/- 15 0.011063 0.6891519 0.48061 0.782402 0.575451

S2-genes differentially expressed across region.xls Page 15 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI LPHN1 latrophilin 1 3273 +/- 149 2840 +/- 165 3901 +/- 181 3300 +/- 124 0.001197 -0.080279 -0.023978 0.0840345 0.2816035 LPIN2 lipin 2 2047 +/- 82 1800 +/- 219 2374 +/- 156 1943 +/- 168 0.013782 -0.328971 -0.094644 0.3390454 0.203824 LPL lipoprotein lipase 464 +/- 38 692 +/- 112 1711 +/- 182 1760 +/- 102 1.69E-06 0.1031246 0.819531 0.1659804 0.610734 LRBA LPS-responsive vesicle trafficking, beach and anchor containing 170 +/- 21 175 +/- 6 242 +/- 14 194 +/- 12 0.019703 0.8467267 -0.031553 0.6117714 0.0536147 LRCH2 leucine-rich repeats and calponin homology (CH) domain containing 2 1068 +/- 87 859 +/- 108 1283 +/- 45 1287 +/- 47 0.00239 -0.165552 0.958238 0.1014569 0.4056246 LRCH3 leucine-rich repeats and calponin homology (CH) domain containing 3 203 +/- 21 255 +/- 25 251 +/- 27 310 +/- 23 0.049952 0.1432771 0.124876 0.1976662 0.3874693 LRCH4 leucine-rich repeats and calponin homology (CH) domain containing 4 282 +/- 15 296 +/- 16 392 +/- 27 354 +/- 34 0.00137 0.5451383 -0.403157 0.533911 0.6711157 LRFN4 leucine rich repeat and fibronectin type III domain containing 4 952 +/- 54 811 +/- 44 1099 +/- 61 1016 +/- 37 0.005731 -0.071285 -0.27678 0.0654939 0.401723 LRP1 low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor) 2003 +/- 63 1954 +/- 76 2328 +/- 60 2621 +/- 151 0.000374 -0.630331 0.127871 0.3776362 0.6146987 LRP3 low density lipoprotein receptor-related protein 3 1738 +/- 85 1898 +/- 194 1955 +/- 148 2034 +/- 76 0.0495 0.4749156 0.646848 0.8507438 0.6838935 LRRC2 leucine rich repeat containing 2 106 +/- 15 88 +/- 11 234 +/- 37 148 +/- 31 0.005304 -0.353719 -0.109006 0.3587107 0.1228812 LRRC28 leucine rich repeat containing 28 389 +/- 32 357 +/- 24 478 +/- 25 395 +/- 14 0.02138 -0.447189 -0.021643 0.3077422 0.1417298 LRRC35 leucine rich repeat containing 35 261 +/- 29 272 +/- 27 313 +/- 23 359 +/- 19 0.000303 0.7864287 0.163857 0.7922677 0.7283294 LRRC3B leucine rich repeat containing 3B 1060 +/- 61 844 +/- 59 1402 +/- 91 1140 +/- 58 0.001031 -0.029331 -0.040665 0.0542119 0.2739959 LRRC41 leucine rich repeat containing 41 863 +/- 56 818 +/- 62 1000 +/- 29 967 +/- 36 0.038964 -0.601833 -0.497717 0.3503785 0.6995281 LRRC4B leucine rich repeat containing 4B 824 +/- 116 770 +/- 31 1338 +/- 49 1398 +/- 68 3.91E-06 -0.666631 0.496685 0.4995362 0.7855827 LRRC55 Leucine rich repeat containing 55 145 +/- 27 159 +/- 14 292 +/- 35 260 +/- 20 0.00261 0.6577763 -0.455844 0.8431364 0.6451832 LRRC57 leucine rich repeat containing 57 227 +/- 50 241 +/- 59 519 +/- 50 505 +/- 22 7.42E-05 0.861464 -0.812119 0.8970009 0.6480956 LRRC61 leucine rich repeat containing 61 283 +/- 25 252 +/- 17 366 +/- 34 325 +/- 19 0.000281 -0.330489 -0.317726 0.5487117 0.469245 LRRC8A leucine rich repeat containing 8 family, member A 317 +/- 17 285 +/- 28 411 +/- 39 345 +/- 27 0.004224 -0.377667 -0.204792 0.4588345 0.3172088 LRRFIP1 leucine rich repeat (in FLII) interacting protein 1 1529 +/- 101 1224 +/- 53 1604 +/- 56 1461 +/- 75 0.036357 -0.0299 -0.167583 0.0459792 0.224864 LRRN1 leucine rich repeat neuronal 1 2089 +/- 141 1602 +/- 137 2532 +/- 162 1964 +/- 83 0.003337 -0.032954 -0.015981 0.0543872 0.048776 LRRN5 leucine rich repeat neuronal 5 1461 +/- 68 1120 +/- 25 1554 +/- 45 1289 +/- 57 0.017935 -0.002904 -0.006257 0.0073701 0.0648862 LRRN6C Leucine rich repeat neuronal 6C 399 +/- 94 458 +/- 120 1054 +/- 98 1154 +/- 101 0.000404 0.7091207 0.494246 0.9091991 0.3626123 LRTM2 leucine-rich repeats and transmembrane domains 2 686 +/- 106 669 +/- 40 1026 +/- 68 956 +/- 44 0.004322 -0.885224 -0.416184 0.7653357 0.6197482 LSG1 large subunit GTPase 1 homolog (S. cerevisiae) 860 +/- 66 708 +/- 50 937 +/- 47 835 +/- 22 0.026655 -0.099076 -0.088301 0.0872797 0.0926958 LSM12 LSM12 homolog (S. cerevisiae) 860 +/- 30 755 +/- 41 912 +/- 33 911 +/- 68 0.017168 -0.06857 -0.98211 0.1320416 0.9456833 LSM5 LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) 860 +/- 61 808 +/- 19 1126 +/- 50 958 +/- 52 6.31E-05 -0.44519 -0.045762 0.5446384 0.0887925 LSS lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) 109 +/- 28 144 +/- 20 177 +/- 34 182 +/- 28 0.032032 0.3303026 0.906594 0.9343314 0.8774983 LUC7L LUC7-like (S. cerevisiae) 849 +/- 108 935 +/- 58 1181 +/- 114 1112 +/- 46 0.001695 0.5065231 -0.594731 0.5554497 0.9725307 LUC7L2 LUC7-like 2 (S. cerevisiae) 465 +/- 27 434 +/- 20 498 +/- 28 468 +/- 26 0.048281 -0.365101 -0.448684 0.515575 0.2894865 LY6E lymphocyte antigen 6 complex, locus E 3030 +/- 326 2371 +/- 331 3650 +/- 198 3326 +/- 204 0.001099 -0.187069 -0.282981 0.1628787 0.2247906 LY6G5C lymphocyte antigen 6 complex, locus G5C 161 +/- 14 176 +/- 20 205 +/- 22 217 +/- 34 0.038885 0.5383382 0.768042 0.4038461 0.3150411 LYNX1 Ly6/neurotoxin 1 3602 +/- 286 2910 +/- 272 3730 +/- 134 3698 +/- 394 0.044527 -0.109859 -0.942264 0.0699691 0.5168291 LYPLA1 lysophospholipase I 367 +/- 48 344 +/- 44 492 +/- 38 427 +/- 11 0.012604 -0.722277 -0.154767 0.7439144 0.0446651 LYPLAL1 Lysophospholipase-like 1 1724 +/- 216 2920 +/- 69 2065 +/- 232 3308 +/- 85 0.00125 0.0018445 0.002086 0.0113258 0.0342218 LZTFL1 leucine zipper transcription factor-like 1 99 +/- 8 113 +/- 8 131 +/- 17 156 +/- 7 0.001652 0.2556093 0.203118 0.4647294 0.7150796 M6PR mannose-6-phosphate receptor (cation dependent) 2454 +/- 102 2245 +/- 88 2610 +/- 102 2447 +/- 77 0.016454 -0.153424 -0.235769 0.0925325 0.3412548 MAB21L2 mab-21-like 2 (C. elegans) 86 +/- 15 117 +/- 25 178 +/- 18 171 +/- 14 0.000164 0.3357437 -0.759375 0.4536169 0.8032387 MADD MAP-kinase activating death domain 2078 +/- 156 1974 +/- 159 2452 +/- 135 2445 +/- 162 9.92E-05 -0.651835 -0.971254 0.2608435 0.4870023 MAGEE1 melanoma antigen family E, 1 1315 +/- 90 1045 +/- 85 1385 +/- 82 1313 +/- 89 0.032925 -0.054496 -0.568035 0.0144637 0.2644807 MAGEF1 melanoma antigen family F, 1 1035 +/- 43 1097 +/- 24 1133 +/- 67 1264 +/- 79 0.037275 0.243333 0.243475 0.4844176 0.2357351 MAGEH1 melanoma antigen family H, 1 5195 +/- 193 4645 +/- 235 6391 +/- 306 5229 +/- 199 0.003344 -0.101413 -0.012417 0.1068453 0.0896327 MAGI2 membrane associated guanylate kinase, WW and PDZ domain containing 2 202 +/- 32 221 +/- 30 363 +/- 50 346 +/- 28 0.000557 0.6772391 -0.785633 0.5898564 0.6563832 MAK10 MAK10 homolog, amino-acid N-acetyltransferase subunit, (S. cerevisiae) 584 +/- 72 678 +/- 47 636 +/- 47 726 +/- 41 0.03569 0.3052668 0.183323 0.2341747 0.2930989

S2-genes differentially expressed across region.xls Page 16 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI MANBA mannosidase, beta A, lysosomal 160 +/- 11 169 +/- 8 205 +/- 12 201 +/- 11 0.014028 0.5022976 -0.824881 0.631972 0.6221121 MAP1LC3Amicrotubule-associated protein 1 light chain 3 alpha 3967 +/- 274 2363 +/- 245 4800 +/- 253 2961 +/- 404 7.47E-05 -0.001442 -0.006366 0.0598346 0.1636142 MAP2K2 mitogen-activated protein kinase kinase 2 356 +/- 31 447 +/- 42 602 +/- 25 669 +/- 29 2.08E-05 0.113189 0.116597 0.3058045 0.3221498 MAP3K5 mitogen-activated protein kinase kinase kinase 5 1628 +/- 220 1557 +/- 187 1959 +/- 115 2245 +/- 101 0.001064 -0.811413 0.094242 0.6049975 0.1478791 MAPK1 mitogen-activated protein kinase 1 10274 +/- 704 8702 +/- 613 10851 +/- 475 10382 +/- 169 0.027474 -0.123569 -0.387302 0.0685587 0.3118207 MAPK8 mitogen-activated protein kinase 8 222 +/- 17 180 +/- 11 286 +/- 23 237 +/- 17 0.003571 -0.070726 -0.122021 0.0853678 0.3060009 MAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 5349 +/- 300 4874 +/- 259 6786 +/- 156 6811 +/- 183 2.9E-06 -0.258705 0.919258 0.1535892 0.5778675 MAPK8IP3 mitogen-activated protein kinase 8 interacting protein 3 1073 +/- 40 1036 +/- 39 1922 +/- 109 1927 +/- 87 1.18E-08 -0.530567 0.971108 0.2939765 0.7712267 MAPRE3 microtubule-associated protein, RP/EB family, member 3 3330 +/- 90 2945 +/- 89 3841 +/- 102 3654 +/- 41 7.86E-06 -0.012243 -0.134442 0.0240235 0.3335935 MARCH7 Membrane-associated ring finger (C3HC4) 7 78 +/- 9 107 +/- 10 117 +/- 12 120 +/- 14 0.010726 0.0589768 0.883444 0.0219383 0.2960323 MARK2 MAP/microtubule affinity-regulating kinase 2 823 +/- 33 702 +/- 30 941 +/- 40 765 +/- 25 0.022501 -0.023036 -0.005707 0.1351836 0.132393 MARS methionine-tRNA synthetase 1159 +/- 62 1049 +/- 63 1287 +/- 56 1247 +/- 41 0.00513 -0.242528 -0.578667 0.1757808 0.3967699 MAT2A methionine adenosyltransferase II, alpha 1133 +/- 111 1391 +/- 164 1459 +/- 136 1761 +/- 98 0.043784 0.2260081 0.107641 0.1227169 0.2084369 MAT2B methionine adenosyltransferase II, beta 1924 +/- 121 3545 +/- 199 2623 +/- 156 3789 +/- 141 0.006021 9.944E-05 0.00036 0.0073799 0.0041307 MBNL2 Muscleblind-like 2 (Drosophila) 431 +/- 36 550 +/- 27 566 +/- 22 603 +/- 44 0.010202 0.0275284 0.492126 0.0026829 0.0633907 MCF2 MCF.2 cell line derived transforming sequence 162 +/- 25 145 +/- 8 292 +/- 36 245 +/- 29 5.27E-05 -0.540855 -0.337597 0.6234139 0.3495585 MCF2L MCF.2 cell line derived transforming sequence-like 169 +/- 11 113 +/- 10 232 +/- 24 135 +/- 8 0.008463 -0.003333 -0.007613 0.033675 0.0679351 MCFD2 multiple coagulation factor deficiency 2 2292 +/- 120 2106 +/- 122 2550 +/- 85 2403 +/- 53 0.010072 -0.303654 -0.181778 0.154751 0.0872331 MCM4 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) 187 +/- 13 208 +/- 18 265 +/- 17 288 +/- 15 0.000145 0.3725244 0.328651 0.8830199 0.5398512 MCPH1 microcephaly, primary autosomal recessive 1 138 +/- 10 168 +/- 10 193 +/- 10 187 +/- 14 0.006829 0.0657567 -0.750027 0.1742722 0.6854824 MDM1 Mdm4, transformed 3T3 cell double minute 1, p53 binding protein (mouse) 458 +/- 30 398 +/- 37 553 +/- 33 459 +/- 37 0.010915 -0.232939 -0.093504 0.6703842 0.3530479 ME1 malic enzyme 1, NADP(+)-dependent, cytosolic 1348 +/- 68 1454 +/- 99 1706 +/- 95 1593 +/- 64 0.01077 0.4040871 -0.349194 0.1012746 0.9352921 MED12L Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae)-like 276 +/- 27 317 +/- 34 447 +/- 40 495 +/- 51 0.002238 0.3669759 0.482094 0.3684587 0.1847388 MEGF8 multiple EGF-like-domains 8 382 +/- 42 348 +/- 25 503 +/- 22 461 +/- 30 0.000986 -0.511495 -0.292769 0.324304 0.1859379 MEIS3P1 Meis1 homolog 3 (mouse) pseudogene 1 175 +/- 34 118 +/- 10 239 +/- 25 167 +/- 18 0.009456 -0.158737 -0.043823 0.2176017 0.0063298 MESP2 mesoderm posterior 2 homolog (mouse) 412 +/- 70 410 +/- 59 474 +/- 83 426 +/- 73 0.021265 -0.984394 -0.673635 0.8656661 0.436771 MEST mesoderm specific transcript homolog (mouse) 2485 +/- 318 2364 +/- 191 3814 +/- 151 3349 +/- 120 0.00046 -0.752188 -0.039852 0.8600518 0.0791152 METTL7A methyltransferase like 7A 306 +/- 63 569 +/- 51 420 +/- 87 661 +/- 102 0.006533 0.009059 0.107987 0.0092362 0.0722464 METTL7B methyltransferase like 7B 719 +/- 115 846 +/- 122 1029 +/- 72 936 +/- 44 0.013554 0.4659878 -0.301892 0.3801525 0.734737 MFAP5 microfibrillar associated protein 5 89 +/- 44 16 +/- 7 273 +/- 67 350 +/- 69 6.28E-05 -0.15794 0.443624 0.3644509 0.8945759 MFGE8 Milk fat globule-EGF factor 8 protein 258 +/- 26 312 +/- 40 353 +/- 27 319 +/- 33 0.006206 0.2847829 -0.450712 0.2658975 0.071113 MFSD2 major facilitator superfamily domain containing 2 579 +/- 26 656 +/- 46 733 +/- 47 698 +/- 77 0.0101 0.1862206 -0.705865 0.4158092 0.1609365 MFSD4 major facilitator superfamily domain containing 4 1487 +/- 177 1155 +/- 97 1969 +/- 109 1846 +/- 21 4.65E-05 -0.139953 -0.315991 0.0871056 0.3532448 MGAT4B mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B 1582 +/- 45 1343 +/- 116 1738 +/- 117 1588 +/- 157 0.017236 -0.101043 -0.466262 0.0043315 0.1194114 MGC15476thymus expressed gene 3-like 4612 +/- 295 4262 +/- 199 5026 +/- 230 5010 +/- 220 0.008135 -0.350424 -0.96139 0.1596113 0.9112123 MGEA5 meningioma expressed antigen 5 (hyaluronidase) 945 +/- 136 829 +/- 65 1149 +/- 90 1013 +/- 47 0.002231 -0.466572 -0.220947 0.5413074 0.2266776 MGRN1 mahogunin, ring finger 1 884 +/- 55 887 +/- 59 1037 +/- 58 1015 +/- 73 0.001422 0.9741278 -0.821427 0.8908812 0.8041259 MIER1 mesoderm induction early response 1 homolog (Xenopus laevis) 445 +/- 23 518 +/- 23 603 +/- 34 526 +/- 34 0.029382 0.0521286 -0.13956 0.1971887 0.0703255 MIER2 mesoderm induction early response 1, family member 2 361 +/- 19 357 +/- 15 431 +/- 35 396 +/- 17 0.036495 -0.856862 -0.400533 0.9324747 0.2267353 MINK1 misshapen-like kinase 1 (zebrafish) 4263 +/- 507 3750 +/- 335 6232 +/- 524 5580 +/- 328 0.000174 -0.420875 -0.321407 0.4264203 0.7196405 MKRN1 makorin, ring finger protein, 1 1477 +/- 115 1219 +/- 47 1585 +/- 102 1555 +/- 49 0.043362 -0.079717 -0.798288 0.061871 0.791392 MKRN2 makorin, ring finger protein, 2 562 +/- 28 628 +/- 58 747 +/- 46 785 +/- 43 0.000747 0.3405765 0.565667 0.6464234 0.9149528 MLC1 megalencephalic leukoencephalopathy with subcortical cysts 1 2374 +/- 112 2737 +/- 163 2567 +/- 136 2912 +/- 205 0.045387 0.1000663 0.202745 0.2221613 0.8221101 MLLT10 Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated 261 +/- 16 374 +/- 37 406 +/- 32 426 +/- 73 0.008615 0.0273662 0.811362 0.0087313 0.4124782 MLPH melanophilin 164 +/- 33 243 +/- 35 237 +/- 27 278 +/- 23 0.045564 0.1295967 0.273809 0.3349234 0.3182119

S2-genes differentially expressed across region.xls Page 17 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI MLXIP MLX interacting protein 717 +/- 34 798 +/- 38 1173 +/- 49 1202 +/- 47 2.35E-06 0.1473828 0.676798 0.31844 0.3349351 MMP16 matrix metallopeptidase 16 (membrane-inserted) 267 +/- 38 205 +/- 20 387 +/- 31 308 +/- 36 0.000527 -0.191611 -0.135813 0.5045814 0.1127163 MMP17 matrix metallopeptidase 17 (membrane-inserted) 318 +/- 39 257 +/- 33 434 +/- 49 367 +/- 40 0.009736 -0.26276 -0.316446 0.3300199 0.5251296 MMS19L MMS19-like (MET18 homolog, S. cerevisiae) 675 +/- 24 691 +/- 38 802 +/- 25 816 +/- 22 0.002354 0.7298489 0.687312 0.7606622 0.8082316 MNT MAX binding protein 2071 +/- 128 2142 +/- 150 2566 +/- 153 2514 +/- 84 0.002494 0.7258884 -0.773544 0.8901995 0.9744644 MOBKL1A MOB1, Mps One Binder kinase activator-like 1A (yeast) 1079 +/- 157 903 +/- 143 1512 +/- 68 1278 +/- 101 0.008659 -0.427334 -0.095545 0.5215872 0.3940588 MOBKL2A MOB1, Mps One Binder kinase activator-like 2A (yeast) 166 +/- 17 108 +/- 15 199 +/- 13 144 +/- 22 0.03428 -0.030366 -0.067489 0.1776544 0.0137142 MORN2 MORN repeat containing 2 641 +/- 58 699 +/- 20 708 +/- 14 801 +/- 49 0.040355 0.3765853 0.127411 0.1600465 0.0285689 MOXD1 monooxygenase, DBH-like 1 1815 +/- 225 2226 +/- 115 2505 +/- 340 2767 +/- 213 0.017494 0.1449141 0.531957 0.211907 0.8288417 MPHOSPHM-phase phosphoprotein 9 810 +/- 122 669 +/- 84 903 +/- 62 804 +/- 68 0.045125 -0.364047 -0.308987 0.3016514 0.180062 MPI Mannose phosphate isomerase 303 +/- 26 272 +/- 31 341 +/- 16 352 +/- 22 0.029129 -0.455689 0.702325 0.91808 0.3072803 MPP2 membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2) 313 +/- 25 334 +/- 27 499 +/- 39 536 +/- 41 0.000159 0.578992 0.536558 0.9397736 0.6852628 MPP6 membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) 324 +/- 28 239 +/- 22 340 +/- 27 328 +/- 27 0.053454 -0.038754 -0.752353 0.0603883 0.912007 MPP7 membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) 84 +/- 38 106 +/- 21 220 +/- 29 254 +/- 18 2.58E-05 0.6144646 0.337363 0.5966691 0.246474 MPPED2 metallophosphoesterase domain containing 2 607 +/- 23 730 +/- 110 1329 +/- 26 1446 +/- 86 1.26E-10 0.3212101 0.254058 0.590878 0.6425262 MRAS muscle RAS oncogene homolog 437 +/- 31 419 +/- 16 563 +/- 36 606 +/- 31 0.000115 -0.61239 0.392977 0.8955626 0.324905 MRFAP1L1Morf4 family associated protein 1-like 1 2399 +/- 97 2557 +/- 45 2605 +/- 103 2784 +/- 128 0.016852 0.1837818 0.308081 0.3973423 0.6958509 MRPL11 mitochondrial ribosomal protein L11 1205 +/- 44 1225 +/- 86 1294 +/- 25 1385 +/- 113 0.00228 0.8425577 0.473353 0.3533103 0.6511952 MRPL3 mitochondrial ribosomal protein L3 781 +/- 81 819 +/- 36 903 +/- 64 1004 +/- 53 0.003113 0.6800191 0.25401 0.6926698 0.117146 MRPL39 mitochondrial ribosomal protein L39 473 +/- 27 523 +/- 19 566 +/- 19 640 +/- 39 0.000183 0.1679329 0.144294 0.3338956 0.3832933 MRPL54 mitochondrial ribosomal protein L54 455 +/- 104 726 +/- 46 610 +/- 107 982 +/- 113 0.004144 0.049292 0.041066 0.0545014 0.0413921 MRPL9 mitochondrial ribosomal protein L9 575 +/- 46 549 +/- 31 651 +/- 28 737 +/- 74 0.003324 -0.639284 0.32733 0.3500354 0.4619352 MRPS10 mitochondrial ribosomal protein S10 356 +/- 43 360 +/- 37 469 +/- 31 497 +/- 43 0.004835 0.9399476 0.606438 0.9899411 0.7233633 MRPS25 mitochondrial ribosomal protein S25 218 +/- 26 254 +/- 22 280 +/- 29 300 +/- 28 0.000808 0.3162447 0.636723 0.5897029 0.808781 MRPS36 mitochondrial ribosomal protein S36 958 +/- 65 818 +/- 28 1027 +/- 74 912 +/- 20 0.01874 -0.089233 -0.185864 0.1237787 0.4673682 MRVI1 murine retrovirus integration site 1 homolog 457 +/- 46 524 +/- 51 613 +/- 45 594 +/- 61 9.99E-05 0.3481361 -0.808375 0.6697419 0.320923 MSL2L1 male-specific lethal 2-like 1 (Drosophila) 398 +/- 18 459 +/- 49 464 +/- 13 511 +/- 53 7.62E-05 0.284194 0.430695 0.2878629 0.4890039 MSRB3 methionine sulfoxide reductase B3 117 +/- 13 133 +/- 28 266 +/- 24 253 +/- 13 5.95E-05 0.6210646 -0.649591 0.9291476 0.766074 MST101 MSTP101 779 +/- 39 705 +/- 21 871 +/- 42 785 +/- 45 0.039148 -0.13329 -0.195258 0.1290803 0.2686055 MTA1 metastasis associated 1 571 +/- 15 536 +/- 18 732 +/- 26 656 +/- 41 0.000472 -0.166695 -0.159407 0.1660907 0.0126497 MTA3 metastasis associated 1 family, member 3 299 +/- 14 293 +/- 29 367 +/- 15 346 +/- 28 0.001465 -0.870304 -0.537243 0.5785176 0.5012516 MTCH2 mitochondrial carrier homolog 2 (C. elegans) 920 +/- 29 979 +/- 56 1087 +/- 41 1002 +/- 69 0.013021 0.3770053 -0.323832 0.6495518 0.0216887 MTF1 metal-regulatory transcription factor 1 916 +/- 47 905 +/- 59 1010 +/- 45 1012 +/- 81 0.017789 -0.876415 0.980004 0.9970961 0.9823894 MTMR7 myotubularin related protein 7 514 +/- 85 382 +/- 44 710 +/- 69 724 +/- 78 0.001643 -0.206931 0.898316 0.1320345 0.922503 MTPN myotrophin 9221 +/- 411 8161 +/- 473 9817 +/- 285 9520 +/- 335 0.017772 -0.122391 -0.517672 0.0516526 0.6858339 MUM1L1 melanoma associated antigen (mutated) 1-like 1 981 +/- 146 739 +/- 116 1184 +/- 67 1198 +/- 168 0.005044 -0.224307 0.937025 0.1619051 0.9637021 MXD4 MAX dimerization protein 4 549 +/- 32 530 +/- 56 609 +/- 44 590 +/- 30 0.037312 -0.774293 -0.734029 0.874651 0.7037527 MXRA7 matrix-remodelling associated 7 203 +/- 23 119 +/- 18 218 +/- 27 164 +/- 25 0.017414 -0.018307 -0.17431 0.0471686 0.2162351 MYCBP2 MYC binding protein 2 1656 +/- 229 1566 +/- 167 2789 +/- 279 2333 +/- 274 0.002512 -0.758852 -0.273458 0.6861531 0.8487281 MYO5B myosin VB 327 +/- 86 237 +/- 59 476 +/- 56 404 +/- 35 0.023161 -0.406118 -0.306366 0.2940251 0.4447915 MYT1L myelin transcription factor 1-like 170 +/- 37 93 +/- 8 240 +/- 30 264 +/- 19 0.002595 -0.095685 0.522797 0.0965847 0.4031136 N4BP2 Nedd4 binding protein 2 631 +/- 115 776 +/- 103 1155 +/- 110 1168 +/- 144 0.007361 0.3691606 0.945 0.3571545 0.7668876 NAALAD2 N-acetylated alpha-linked acidic dipeptidase 2 111 +/- 18 179 +/- 41 512 +/- 50 532 +/- 42 7.91E-06 0.1688075 0.758886 0.2949729 0.6330303 NAB2 NGFI-A binding protein 2 (EGR1 binding protein 2) 419 +/- 51 363 +/- 38 552 +/- 20 533 +/- 21 0.001168 -0.39681 -0.521498 0.2266163 0.6278468 NACA nascent-polypeptide-associated complex alpha polypeptide 13050 +/- 239 13192 +/- 270 13999 +/- 370 14323 +/- 262 0.003425 0.7028534 0.493872 0.639576 0.4010861

S2-genes differentially expressed across region.xls Page 18 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI NAG neuroblastoma-amplified protein 98 +/- 12 158 +/- 21 165 +/- 17 192 +/- 26 0.046839 0.0385576 0.425889 0.014356 0.4542652 NANOS1 nanos homolog 1 (Drosophila) 291 +/- 47 301 +/- 59 618 +/- 64 529 +/- 35 0.001251 0.8989935 -0.257588 0.8131065 0.4376344 NAP1L2 nucleosome assembly protein 1-like 2 5107 +/- 280 4441 +/- 157 5850 +/- 309 5272 +/- 263 0.013288 -0.072808 -0.187873 0.0257402 0.3462678 NAPA N-ethylmaleimide-sensitive factor attachment protein, alpha 1686 +/- 112 1839 +/- 100 2364 +/- 17 2358 +/- 74 1.41E-05 0.3343235 -0.934933 0.5760743 0.8215754 NAPB N-ethylmaleimide-sensitive factor attachment protein, beta 177 +/- 26 406 +/- 74 348 +/- 67 475 +/- 68 0.013533 0.0261124 0.218482 0.1134007 0.3241578 NAPG N-ethylmaleimide-sensitive factor attachment protein, gamma 2846 +/- 59 2625 +/- 247 3514 +/- 149 3585 +/- 331 2.77E-05 -0.418425 0.851188 0.1632512 0.8997516 NARG1 NMDA receptor regulated 1 465 +/- 57 616 +/- 59 591 +/- 31 710 +/- 79 0.010074 0.0961971 0.21605 0.0884586 0.1912418 NARG1L NMDA receptor regulated 1-like 453 +/- 43 495 +/- 23 551 +/- 12 524 +/- 29 0.036716 0.4144784 -0.414229 0.2567032 0.5546383 NAT11 N-acetyltransferase 11 331 +/- 40 324 +/- 20 400 +/- 33 411 +/- 18 0.001232 -0.87335 0.784123 0.9556599 0.8155108 NBEA neurobeachin 1246 +/- 191 967 +/- 153 1825 +/- 171 1607 +/- 53 9.12E-05 -0.282402 -0.270868 0.1198024 0.2284989 NBL1 neuroblastoma, suppression of tumorigenicity 1 1787 +/- 164 1655 +/- 135 2106 +/- 144 2115 +/- 73 0.001221 -0.549548 0.955041 0.3781639 0.7789883 NBR1 neighbor of BRCA1 gene 1 1100 +/- 31 1283 +/- 49 1205 +/- 34 1320 +/- 16 0.027118 0.0122479 0.018009 0.0015849 0.086231 NCDN neurochondrin 1739 +/- 322 1197 +/- 79 3697 +/- 273 3599 +/- 184 2.32E-07 -0.157264 -0.774503 0.2520294 0.9843508 NCKAP1 NCK-associated protein 1 4172 +/- 479 3691 +/- 189 5125 +/- 363 4600 +/- 295 0.006711 -0.383198 -0.290745 0.4365796 0.9712606 NCOA3 Nuclear receptor coactivator 3 89 +/- 15 124 +/- 9 166 +/- 12 180 +/- 18 0.000271 0.0723115 0.528324 0.1500254 0.694608 NCOA5 nuclear receptor coactivator 5 279 +/- 3 222 +/- 19 348 +/- 18 305 +/- 13 6.26E-05 -0.02781 -0.088613 0.0763502 0.2254017 NCOA6IP nuclear receptor coactivator 6 interacting protein 483 +/- 36 554 +/- 44 578 +/- 42 566 +/- 41 0.041829 0.2406947 -0.837756 0.398501 0.5393954 NCOR2 nuclear receptor co-repressor 2 541 +/- 62 580 +/- 42 1088 +/- 67 1184 +/- 80 3.38E-06 0.6235234 0.388699 0.8987635 0.3801074 NDRG4 NDRG family member 4 12069 +/- 928 10040 +/- 881 14103 +/- 758 14395 +/- 580 0.00016 -0.144083 0.766934 0.0346613 0.7670733 NDUFS8 NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductas 3356 +/- 146 2942 +/- 127 3465 +/- 97 3358 +/- 116 0.031185 -0.058368 -0.498414 0.0373897 0.2724169 NECAP1 NECAP endocytosis associated 1 5116 +/- 339 4448 +/- 326 5799 +/- 190 5863 +/- 204 0.000488 -0.185835 0.8237 0.0812145 0.8729847 NEK6 NIMA (never in mitosis gene a)-related kinase 6 812 +/- 36 732 +/- 50 839 +/- 58 877 +/- 67 0.046974 -0.228245 0.680198 0.0955072 0.5734199 NELL1 NEL-like 1 (chicken) 2297 +/- 225 1832 +/- 209 3169 +/- 220 2599 +/- 185 1.52E-05 -0.160379 -0.078758 0.1259619 0.1806587 NELL2 NEL-like 2 (chicken) 3619 +/- 287 3012 +/- 285 4656 +/- 394 3639 +/- 278 0.025357 -0.16434 -0.065561 0.3255075 0.4702339 NEUROD1 neurogenic differentiation 1 3513 +/- 1010 2385 +/- 631 4285 +/- 253 3926 +/- 228 0.049023 -0.370107 -0.320447 0.3538158 0.4492102 NFIC nuclear factor I/C (CCAAT-binding transcription factor) 198 +/- 10 244 +/- 11 275 +/- 22 264 +/- 14 0.015033 0.0111857 -0.689607 0.0046001 0.8942694 NFIL3 nuclear factor, interleukin 3 regulated 495 +/- 40 462 +/- 38 822 +/- 51 669 +/- 29 0.000782 -0.562878 -0.032292 0.5630724 0.1254024 NFIX nuclear factor I/X (CCAAT-binding transcription factor) 215 +/- 16 195 +/- 13 347 +/- 26 287 +/- 15 0.000259 -0.358422 -0.083323 0.4906664 0.1183526 NFKB1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) 175 +/- 8 201 +/- 13 206 +/- 6 207 +/- 7 0.038789 0.1254885 0.92693 0.0277622 0.5966369 NFKBIL1 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 309 +/- 20 323 +/- 16 370 +/- 9 356 +/- 34 0.02112 0.595381 -0.705425 0.4148677 0.5578979 NFXL1 nuclear transcription factor, X-box binding-like 1 253 +/- 22 233 +/- 20 303 +/- 28 251 +/- 30 0.028711 -0.517945 -0.234563 0.7187681 0.672197 NIPBL Nipped-B homolog (Drosophila) 651 +/- 32 814 +/- 41 781 +/- 26 815 +/- 32 0.035838 0.0111975 0.434143 0.0066467 0.6387933 NIPSNAP3 nipsnap homolog 3B (C. elegans) 376 +/- 28 344 +/- 40 429 +/- 39 393 +/- 44 0.036614 -0.522689 -0.560903 0.2714854 0.1574623 NISCH nischarin 262 +/- 23 343 +/- 27 377 +/- 20 423 +/- 44 0.008463 0.0475203 0.38318 0.1719379 0.043231 NKAP NF-kappaB activating protein 839 +/- 49 818 +/- 40 910 +/- 38 909 +/- 26 0.002981 -0.753006 -0.984875 0.9589675 0.6519798 NKD2 naked cuticle homolog 2 (Drosophila) 229 +/- 12 225 +/- 18 335 +/- 22 274 +/- 16 0.00102 -0.873328 -0.053989 0.951125 0.1893995 NKTR natural killer-tumor recognition sequence 456 +/- 55 637 +/- 40 647 +/- 63 788 +/- 65 0.000111 0.024757 0.155518 0.0520207 0.3418644 NLGN2 neuroligin 2 3341 +/- 188 2899 +/- 223 3703 +/- 183 3464 +/- 128 0.007712 -0.161946 -0.313345 0.0405993 0.3029904 NLGN4X neuroligin 4, X-linked 508 +/- 68 429 +/- 65 789 +/- 46 879 +/- 62 1.06E-05 -0.42024 0.276884 0.2797469 0.7043568 NLGN4Y neuroligin 4, Y-linked 170 +/- 27 172 +/- 25 325 +/- 32 341 +/- 35 0.000109 0.9638457 0.733012 0.7659185 0.880029 NLK nemo-like kinase 466 +/- 83 408 +/- 44 662 +/- 86 588 +/- 61 0.008496 -0.559971 -0.50039 0.2209082 0.1324337 NMB neuromedin B 567 +/- 49 552 +/- 48 714 +/- 85 748 +/- 60 0.015368 -0.827904 0.744727 0.401796 0.2734817 NME5 non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase) 104 +/- 6 122 +/- 11 124 +/- 13 140 +/- 10 0.004503 0.1871633 0.34171 0.4481356 0.5136443 NME7 non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase) 497 +/- 29 562 +/- 53 812 +/- 92 632 +/- 91 0.017043 0.3116744 -0.197924 0.2471425 0.3164143 NMNAT3 nicotinamide nucleotide adenylyltransferase 3 330 +/- 40 255 +/- 42 470 +/- 45 414 +/- 33 0.007466 -0.226734 -0.343572 0.2636034 0.334217

S2-genes differentially expressed across region.xls Page 19 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI NMT2 N-myristoyltransferase 2 443 +/- 35 350 +/- 17 594 +/- 32 534 +/- 28 1.87E-05 -0.045269 -0.185807 0.0344018 0.2033577 NOG Noggin 599 +/- 81 458 +/- 67 733 +/- 50 542 +/- 33 0.051327 -0.208994 -0.011914 0.1772806 0.0994863 NOL5A nucleolar protein 5A (56kDa with KKE/D repeat) 2245 +/- 141 2213 +/- 112 2567 +/- 69 2616 +/- 123 0.002654 -0.860991 0.736242 0.9063336 0.5395845 NPAS3 neuronal PAS domain protein 3 566 +/- 42 643 +/- 29 757 +/- 27 753 +/- 32 0.000841 0.1593994 -0.926031 0.2934572 0.1591154 NPDC1 neural proliferation, differentiation and control, 1 4559 +/- 197 3968 +/- 202 5745 +/- 207 5401 +/- 126 3.34E-05 -0.062505 -0.193739 0.0194807 0.319617 NPHP1 nephronophthisis 1 (juvenile) 338 +/- 22 294 +/- 26 541 +/- 52 436 +/- 34 0.000315 -0.226588 -0.129014 0.3839328 0.1181205 NPHP3 nephronophthisis 3 (adolescent) 113 +/- 9 157 +/- 10 147 +/- 18 189 +/- 8 0.005721 0.0088437 0.07045 0.0690173 0.13618 NPNT nephronectin 805 +/- 96 684 +/- 56 1208 +/- 217 758 +/- 30 0.041919 -0.307481 -0.092806 0.3814183 0.2691812 NPTX1 neuronal pentraxin I 8387 +/- 1267 9161 +/- 1287 14648 +/- 897 15790 +/- 811 1.22E-05 0.6773072 0.369852 0.7540747 0.2938702 NPTX2 neuronal pentraxin II 4585 +/- 1217 3276 +/- 1016 6166 +/- 781 6761 +/- 706 0.03526 -0.428822 0.586341 0.3484818 0.5435351 NPTXR neuronal pentraxin receptor 12565 +/- 1977 10967 +/- 1803 16606 +/- 714 16669 +/- 892 0.002955 -0.563599 0.957286 0.4686714 0.9669253 NPY2R neuropeptide Y receptor Y2 107 +/- 19 294 +/- 143 991 +/- 64 1064 +/- 74 2.01E-05 0.248922 0.472566 0.481955 0.5481925 NR1I2 nuclear receptor subfamily 1, group I, member 2 115 +/- 5 95 +/- 10 128 +/- 12 128 +/- 13 0.038964 -0.123811 -0.991496 0.0259035 0.5608694 NR2F1 Nuclear receptor subfamily 2, group F, member 1 3880 +/- 234 4227 +/- 278 4775 +/- 257 5717 +/- 210 0.001232 0.3628789 0.019733 0.6493127 0.043827 NRBF2 nuclear receptor binding factor 2 542 +/- 29 490 +/- 19 617 +/- 19 556 +/- 29 0.004482 -0.168413 -0.118279 0.2261694 0.1943647 NRCAM neuronal cell adhesion molecule 1212 +/- 154 1214 +/- 127 1400 +/- 88 1697 +/- 126 0.032805 0.9944409 0.093955 0.5839952 0.5416734 NRG2 neuregulin 2 233 +/- 16 227 +/- 24 286 +/- 29 285 +/- 16 0.004614 -0.856168 -0.975767 0.8517187 0.8332187 NRP2 neuropilin 2 178 +/- 27 256 +/- 23 279 +/- 17 347 +/- 12 0.001843 0.0546684 0.010231 0.0441847 0.0569377 NRXN2 neurexin 2 1755 +/- 188 1788 +/- 81 2185 +/- 139 2148 +/- 158 0.000237 0.8762021 -0.863398 0.5134719 0.4395353 NRXN3 neurexin 3 3938 +/- 223 3611 +/- 150 4753 +/- 193 5012 +/- 308 0.000116 -0.254617 0.499592 0.2176793 0.8767913 NSBP1 nucleosomal binding protein 1 223 +/- 25 184 +/- 11 265 +/- 12 267 +/- 18 0.010517 -0.191626 0.948789 0.2883785 0.6356066 NSUN2 NOL1/NOP2/Sun domain family, member 2 521 +/- 29 653 +/- 50 633 +/- 51 677 +/- 39 0.005451 0.0534071 0.513679 0.2984479 0.8637154 NT5DC3 5'-nucleotidase domain containing 3 3937 +/- 446 3377 +/- 456 4650 +/- 234 4550 +/- 238 0.003112 -0.399949 -0.76973 0.2562128 0.6632909 NTF3 neurotrophin 3 180 +/- 45 189 +/- 22 361 +/- 59 312 +/- 30 0.014268 0.8641472 -0.474539 0.9074538 0.5847139 NTNG2 netrin G2 1182 +/- 175 925 +/- 139 2032 +/- 132 2043 +/- 94 1.63E-05 -0.277893 0.94951 0.2466772 0.8993512 NTRK3 neurotrophic tyrosine kinase, receptor, type 3 363 +/- 49 319 +/- 48 524 +/- 16 509 +/- 32 0.000153 -0.542719 -0.678636 0.5654823 0.9532434 NUDT11 nudix (nucleoside diphosphate linked moiety X)-type motif 11 1910 +/- 119 1671 +/- 195 2069 +/- 90 2038 +/- 196 0.039126 -0.324739 -0.891197 0.1912925 0.709447 NUDT17 nudix (nucleoside diphosphate linked moiety X)-type motif 17 128 +/- 5 139 +/- 9 173 +/- 3 168 +/- 14 0.000542 0.3077328 -0.78463 0.0901453 0.3533989 NUDT4 nudix (nucleoside diphosphate linked moiety X)-type motif 4 341 +/- 23 281 +/- 33 505 +/- 25 364 +/- 47 0.000121 -0.167955 -0.036215 0.2096351 0.049617 NUDT6 nudix (nucleoside diphosphate linked moiety X)-type motif 6 221 +/- 25 217 +/- 12 279 +/- 33 237 +/- 23 0.01906 -0.902999 -0.325162 0.7622408 0.8276301 NUFIP1 nuclear fragile X mental retardation protein interacting protein 1 389 +/- 16 344 +/- 13 423 +/- 26 378 +/- 24 0.012366 -0.053026 -0.23428 0.0239729 0.108255 NUFIP2 nuclear fragile X mental retardation protein interacting protein 2 761 +/- 34 710 +/- 36 845 +/- 39 746 +/- 16 0.029363 -0.328267 -0.051374 0.748716 0.0581558 NUMA1 nuclear mitotic apparatus protein 1 102 +/- 11 139 +/- 28 124 +/- 11 171 +/- 22 0.04309 0.2684087 0.108222 0.1979136 0.0481205 NUMBL numb homolog (Drosophila)-like 973 +/- 100 832 +/- 70 1483 +/- 86 1438 +/- 91 4.71E-06 -0.277297 -0.731692 0.116575 0.4496121 NUP50 nucleoporin 50kDa 2247 +/- 143 3427 +/- 362 2766 +/- 251 3193 +/- 321 0.052566 0.0208199 0.325114 0.0368692 0.442796 NUP62 nucleoporin 62kDa 885 +/- 11 1158 +/- 43 1053 +/- 49 1241 +/- 35 0.01417 0.0009882 0.012691 0.0697167 0.041829 NUP85 nucleoporin 85kDa 1138 +/- 76 1108 +/- 81 1222 +/- 25 1286 +/- 25 0.031802 -0.79416 0.101761 0.7198646 0.6097356 NUPL1 nucleoporin like 1 80 +/- 12 133 +/- 19 138 +/- 7 175 +/- 24 0.000357 0.0436761 0.212114 0.2357862 0.8285182 OBSL1 obscurin-like 1 676 +/- 34 758 +/- 38 846 +/- 33 930 +/- 30 0.000343 0.1391008 0.095893 0.3398491 0.1534025 OGDHL oxoglutarate dehydrogenase-like 1566 +/- 124 1440 +/- 147 1713 +/- 83 1788 +/- 117 0.005452 -0.52877 0.615523 0.3348651 0.7775816 OGFRL1 opioid growth factor receptor-like 1 121 +/- 12 168 +/- 17 211 +/- 16 189 +/- 23 0.006054 0.0544192 -0.447763 0.0564482 0.47636 OGT O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypepti 1094 +/- 60 899 +/- 25 1800 +/- 83 1694 +/- 67 4.43E-07 -0.022121 -0.34876 0.1719069 0.6526878 OLFM2 olfactomedin 2 1429 +/- 94 1459 +/- 94 1830 +/- 214 1804 +/- 107 0.002395 0.826934 -0.915851 0.6750257 0.759015 OLFML1 olfactomedin-like 1 106 +/- 22 94 +/- 12 127 +/- 21 99 +/- 7 0.044007 -0.637883 -0.242967 0.7561494 0.2797264 OPRS1 opioid receptor, sigma 1 649 +/- 74 520 +/- 38 828 +/- 61 872 +/- 44 0.002167 -0.16362 0.580699 0.1794188 0.621622

S2-genes differentially expressed across region.xls Page 20 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI OPTN optineurin 1234 +/- 134 1443 +/- 56 1440 +/- 68 1677 +/- 80 0.000947 0.1953036 0.051872 0.2762996 0.044902 ORMDL3 ORM1-like 3 (S. cerevisiae) 240 +/- 23 235 +/- 13 311 +/- 12 273 +/- 13 0.00113 -0.861437 -0.065597 0.6230597 0.2103392 OSBPL7 oxysterol binding protein-like 7 109 +/- 8 132 +/- 8 195 +/- 8 172 +/- 26 0.003122 0.0771645 -0.42948 0.1158777 0.5957631 OSRF osmosis responsive factor 556 +/- 112 499 +/- 61 709 +/- 85 607 +/- 43 0.002391 -0.665699 -0.322839 0.8312407 0.5798275 OVOL1 ovo-like 1(Drosophila) 112 +/- 15 124 +/- 9 157 +/- 14 140 +/- 10 0.044664 0.5287326 -0.361026 0.7448317 0.7150908 P2RX5 purinergic receptor P2X, ligand-gated ion channel, 5 1338 +/- 74 1083 +/- 93 1528 +/- 87 1238 +/- 63 0.025993 -0.058172 -0.025511 0.0974106 0.0695644 P2RY14 purinergic receptor P2Y, G-protein coupled, 14 107 +/- 11 100 +/- 8 175 +/- 23 148 +/- 18 0.001602 -0.588146 -0.370044 0.8603651 0.74963 P4HB procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypepti 387 +/- 57 498 +/- 37 540 +/- 34 637 +/- 39 0.00917 0.1426439 0.092837 0.2291056 0.6851306 PA2G4 proliferation-associated 2G4, 38kDa 712 +/- 69 939 +/- 45 885 +/- 51 1182 +/- 44 0.000332 0.0231989 0.001752 0.0297561 0.0004348 PACSIN1 protein kinase C and casein kinase substrate in neurons 1 6703 +/- 229 7137 +/- 561 8175 +/- 310 8546 +/- 341 0.000473 0.498076 0.442272 0.8546795 0.872812 PAG1 phosphoprotein associated with glycosphingolipid microdomains 1 1306 +/- 191 1142 +/- 178 2064 +/- 236 1954 +/- 144 0.00167 -0.544718 -0.699719 0.4568409 0.9529992 PAIP1 poly(A) binding protein interacting protein 1 2718 +/- 182 2080 +/- 74 3154 +/- 104 2326 +/- 67 0.015668 -0.015324 -0.000136 0.031099 0.0141581 PAK3 P21 (CDKN1A)-activated kinase 3 227 +/- 18 167 +/- 19 271 +/- 16 203 +/- 14 0.052076 -0.048202 -0.010497 0.0506195 0.0772543 PALMD palmdelphin 203 +/- 17 151 +/- 13 248 +/- 26 204 +/- 20 0.000514 -0.039876 -0.211303 0.202666 0.2398417 PAPD1 PAP associated domain containing 1 886 +/- 44 1400 +/- 87 1045 +/- 67 1422 +/- 80 0.027286 0.0009632 0.006476 0.002258 0.0023265 PAPOLG poly(A) polymerase gamma 791 +/- 23 698 +/- 22 821 +/- 24 839 +/- 22 0.005476 -0.014573 0.597447 0.041462 0.8523547 PARK2 Parkinson disease (autosomal recessive, juvenile) 2, parkin 143 +/- 18 177 +/- 24 203 +/- 17 251 +/- 14 0.007899 0.2936315 0.05811 0.5035322 0.0350739 PARP8 poly (ADP-ribose) polymerase family, member 8 406 +/- 45 403 +/- 30 443 +/- 29 519 +/- 18 0.010472 -0.951028 0.053006 0.9421587 0.1955899 PAX6 paired box gene 6 (aniridia, keratitis) 1091 +/- 49 1069 +/- 41 1263 +/- 63 1174 +/- 51 0.010181 -0.73126 -0.302512 0.4929127 0.1817337 PBX2 pre-B-cell leukemia transcription factor 2 132 +/- 18 116 +/- 19 181 +/- 22 166 +/- 11 0.014928 -0.566728 -0.565075 0.3138172 0.7277874 PCBD1 pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear fact 1102 +/- 56 1160 +/- 70 1490 +/- 100 1509 +/- 72 6.79E-05 0.5360118 0.880065 0.6517877 0.8975378 PCBD2 pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear fact 859 +/- 60 907 +/- 30 1075 +/- 79 945 +/- 51 0.020975 0.4912241 -0.204049 0.4636112 0.5532759 PCBP3 poly(rC) binding protein 3 391 +/- 60 520 +/- 48 834 +/- 74 869 +/- 61 0.000428 0.1251358 0.718854 0.0887636 0.8597245 PCDH1 protocadherin 1 (cadherin-like 1) 178 +/- 17 162 +/- 12 240 +/- 16 186 +/- 15 0.001299 -0.439022 -0.039922 0.1526281 0.0256257 PCDH18 protocadherin 18 341 +/- 79 322 +/- 66 770 +/- 76 653 +/- 119 0.001588 -0.858248 -0.434952 0.8253399 0.2472093 PCDHB13 protocadherin beta 13 189 +/- 13 200 +/- 26 291 +/- 20 249 +/- 25 0.003952 0.7113407 -0.227119 0.7238273 0.1904339 PCDHB14 protocadherin beta 14 224 +/- 13 229 +/- 9 342 +/- 16 269 +/- 6 0.001369 0.7321903 -0.004447 0.2135377 0.0333462 PCDHB4 protocadherin beta 4 738 +/- 56 649 +/- 66 806 +/- 71 933 +/- 152 0.023461 -0.328135 0.479213 0.3082233 0.4744722 PCDHB5 protocadherin beta 5 309 +/- 10 309 +/- 25 412 +/- 23 457 +/- 43 8.77E-05 0.989283 0.391303 0.9355144 0.4391364 PCDHGC3 protocadherin gamma subfamily C, 3 5303 +/- 514 4484 +/- 605 5809 +/- 536 4995 +/- 765 0.032893 -0.326987 -0.410816 0.076912 0.1403336 PCGF2 polycomb group ring finger 2 253 +/- 17 158 +/- 15 362 +/- 10 234 +/- 26 2.51E-05 -0.001817 -0.005282 0.0438859 0.0396435 PCLO Piccolo (presynaptic cytomatrix protein) 70 +/- 10 73 +/- 10 145 +/- 14 129 +/- 13 2.24E-05 0.8356554 -0.419956 0.2790293 0.4108378 PCMTD1 protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1 416 +/- 44 527 +/- 52 610 +/- 46 619 +/- 28 0.008168 0.1355157 0.868923 0.1658645 0.9334954 PCSK1N proprotein convertase subtilisin/kexin type 1 inhibitor 4821 +/- 222 4645 +/- 264 6220 +/- 120 6379 +/- 174 2.78E-06 -0.621131 0.473382 0.2943972 0.962108 PCTK1 PCTAIRE protein kinase 1 623 +/- 24 568 +/- 32 793 +/- 32 758 +/- 34 2.5E-05 -0.195485 -0.471522 0.1665812 0.1593949 PCYT1A phosphate cytidylyltransferase 1, choline, alpha 86 +/- 6 95 +/- 8 108 +/- 11 132 +/- 17 0.03225 0.4287356 0.262044 0.3271767 0.162941 PDAP1 PDGFA associated protein 1 1147 +/- 90 1105 +/- 54 1359 +/- 51 1259 +/- 19 0.029413 -0.703854 -0.113849 0.5077124 0.469584 PDCD2 programmed cell death 2 1615 +/- 90 1458 +/- 56 1921 +/- 63 1848 +/- 68 0.000578 -0.173641 -0.454813 0.1904585 0.7437484 PDCD6IP programmed cell death 6 interacting protein 1108 +/- 45 997 +/- 36 1209 +/- 22 1087 +/- 41 0.005832 -0.084134 -0.03722 0.1395256 0.0535386 PDE3B phosphodiesterase 3B, cGMP-inhibited 128 +/- 7 139 +/- 13 172 +/- 13 156 +/- 9 0.023799 0.503465 -0.335448 0.667793 0.3003892 PDE8B phosphodiesterase 8B 248 +/- 54 368 +/- 71 377 +/- 54 570 +/- 63 0.005075 0.2073609 0.04528 0.6610919 0.3982629 PDGFC Platelet derived growth factor C 131 +/- 24 160 +/- 22 158 +/- 20 179 +/- 30 0.04805 0.4023728 0.572921 0.5540251 0.8339853 PDIA3 protein disulfide isomerase family A, member 3 143 +/- 8 130 +/- 18 173 +/- 14 152 +/- 15 0.037991 -0.523208 -0.341739 0.2420777 0.1275054 PDK2 pyruvate dehydrogenase kinase, isozyme 2 858 +/- 49 910 +/- 38 1065 +/- 42 1142 +/- 36 0.000265 0.4284164 0.192543 0.8789779 0.4805409 PDK3 Pyruvate dehydrogenase kinase, isozyme 3 704 +/- 98 960 +/- 111 993 +/- 129 1593 +/- 94 0.000496 0.113882 0.00477 0.4282027 0.0643055

S2-genes differentially expressed across region.xls Page 21 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI PDLIM5 PDZ and LIM domain 5 599 +/- 216 870 +/- 119 731 +/- 217 1228 +/- 86 0.005761 0.3051137 0.074103 0.4120091 0.2290282 PDZD4 PDZ domain containing 4 3783 +/- 310 3565 +/- 446 4446 +/- 316 4425 +/- 290 0.032335 -0.699013 -0.961908 0.4809484 0.9422691 PDZD8 PDZ domain containing 8 271 +/- 60 345 +/- 37 353 +/- 56 445 +/- 22 0.004394 0.321713 0.175416 0.2597872 0.2398167 PEA15 phosphoprotein enriched in astrocytes 15 14228 +/- 407 15719 +/- 171 16261 +/- 383 17626 +/- 635 0.001449 0.0125159 0.110018 0.0156896 0.3490645 PECR peroxisomal trans-2-enoyl-CoA reductase 242 +/- 16 218 +/- 22 292 +/- 26 246 +/- 17 0.01089 -0.396809 -0.183302 0.9023752 0.398901 PELI1 Pellino homolog 1 (Drosophila) 365 +/- 31 387 +/- 31 450 +/- 34 479 +/- 55 0.009326 0.6226172 0.669479 0.4346985 0.1170485 PELI3 pellino homolog 3 (Drosophila) 395 +/- 37 431 +/- 37 464 +/- 27 466 +/- 12 0.001595 0.5145036 0.926785 0.7288256 0.7831766 PER3 Period homolog 3 (Drosophila) 83 +/- 10 104 +/- 11 142 +/- 17 152 +/- 16 0.005783 0.189197 0.689759 0.4690703 0.8291918 PERLD1 per1-like domain containing 1 518 +/- 27 460 +/- 26 554 +/- 25 531 +/- 25 0.047237 -0.153677 -0.53496 0.3604362 0.8646718 PERQ1 PERQ amino acid rich, with GYF domain 1 277 +/- 29 340 +/- 41 386 +/- 30 385 +/- 32 0.026901 0.2373479 -0.977119 0.1391125 0.7945747 PEX1 peroxisome biogenesis factor 1 287 +/- 25 338 +/- 14 427 +/- 38 421 +/- 41 0.013313 0.1115574 -0.927683 0.1595497 0.3825966 PFDN4 prefoldin subunit 4 737 +/- 71 858 +/- 41 809 +/- 83 1017 +/- 19 0.014219 0.178896 0.053249 0.1720676 0.2405397 PFTK1 PFTAIRE protein kinase 1 5855 +/- 484 4985 +/- 417 7553 +/- 181 6831 +/- 397 7.23E-05 -0.203789 -0.151968 0.2039817 0.2849173 PGAM5 phosphoglycerate mutase family member 5 281 +/- 55 207 +/- 18 322 +/- 30 313 +/- 30 0.013395 -0.242154 -0.821408 0.1651589 0.6510883 PGBD1 piggyBac transposable element derived 1 355 +/- 32 424 +/- 35 412 +/- 15 406 +/- 35 0.038967 0.1790127 -0.876439 0.0109031 0.2874278 PGBD3 piggyBac transposable element derived 3 247 +/- 13 262 +/- 17 310 +/- 15 281 +/- 16 0.025233 0.5062626 -0.226667 0.3932836 0.3399674 PGBD5 piggyBac transposable element derived 5 6236 +/- 795 5493 +/- 857 7638 +/- 365 7827 +/- 266 0.004081 -0.539617 0.686516 0.422325 0.9795432 PGLS 6-phosphogluconolactonase 1831 +/- 34 1684 +/- 66 2078 +/- 78 2070 +/- 115 0.000516 -0.085313 -0.953696 0.1252865 0.7999246 PGRMC1 progesterone receptor membrane component 1 2730 +/- 146 2649 +/- 115 3350 +/- 249 2861 +/- 162 0.011082 -0.676088 -0.136712 0.5490754 0.2978251 PHACTR2 phosphatase and actin regulator 2 137 +/- 29 119 +/- 11 169 +/- 31 179 +/- 24 0.012061 -0.569764 0.809339 0.4427445 0.9309784 PHC3 Polyhomeotic like 3 (Drosophila) 83 +/- 5 113 +/- 9 127 +/- 13 127 +/- 17 0.036711 0.0254323 -0.986452 0.0747679 0.4313701 PHF10 PHD finger protein 10 327 +/- 31 419 +/- 15 380 +/- 14 460 +/- 26 0.044383 0.0325161 0.034908 0.0388483 0.1085417 PHKA2 phosphorylase kinase, alpha 2 (liver) 175 +/- 5 160 +/- 11 207 +/- 18 184 +/- 9 0.016274 -0.233042 -0.285452 0.9906048 0.5778136 PHKG2 phosphorylase kinase, gamma 2 (testis) 1152 +/- 43 1029 +/- 48 1264 +/- 53 1159 +/- 41 0.002258 -0.087534 -0.152446 0.1256141 0.223844 PHYHIP phytanoyl-CoA 2-hydroxylase interacting protein 4118 +/- 485 3734 +/- 478 5326 +/- 231 5449 +/- 217 0.001771 -0.585295 0.708588 0.4241773 0.5794907 PIB5PA phosphatidylinositol (4,5) bisphosphate 5-phosphatase, A 467 +/- 42 481 +/- 47 576 +/- 45 639 +/- 49 0.002976 0.8285 0.370199 0.7533565 0.7512232 PIGX phosphatidylinositol glycan, class X 397 +/- 18 439 +/- 27 565 +/- 30 500 +/- 19 0.005052 0.2297089 -0.105512 0.3086768 0.0410702 PIK4CA phosphatidylinositol 4-kinase, catalytic, alpha polypeptide 5386 +/- 366 4686 +/- 391 5724 +/- 179 5401 +/- 76 0.02877 -0.220025 -0.142191 0.1857942 0.3860298 PIP5K1C phosphatidylinositol-4-phosphate 5-kinase, type I, gamma 1274 +/- 207 997 +/- 81 1681 +/- 70 1642 +/- 50 0.001558 -0.254248 -0.665272 0.1665274 0.9126233 PIPOX pipecolic acid oxidase 388 +/- 35 493 +/- 32 447 +/- 29 523 +/- 16 0.046104 0.0521533 0.051334 0.0365581 0.3326665 PITPNC1 Phosphatidylinositol transfer protein, cytoplasmic 1 130 +/- 18 150 +/- 13 145 +/- 6 207 +/- 21 0.04088 0.3841422 0.036598 0.1265199 0.0304022 PKD1 polycystic kidney disease 1 (autosomal dominant) 206 +/- 28 238 +/- 17 397 +/- 27 389 +/- 35 0.000259 0.3630592 -0.845297 0.9366522 0.8629816 PLCB4 phospholipase C, beta 4 168 +/- 17 177 +/- 16 198 +/- 6 207 +/- 9 0.017946 0.7101964 0.439328 0.9362058 0.2352323 PLCH2 phospholipase C, eta 2 370 +/- 51 502 +/- 14 513 +/- 63 801 +/- 59 0.000977 0.0493627 0.008957 0.0679472 0.0876322 PLDN pallidin homolog (mouse) 2387 +/- 83 2511 +/- 102 2561 +/- 45 2750 +/- 87 0.010351 0.3677328 0.100902 0.8406481 0.4915065 PLEKHA2 pleckstrin homology domain containing, family A (phosphoinositide binding specific) mem 1645 +/- 642 2063 +/- 631 4165 +/- 342 4910 +/- 263 0.000506 0.652401 0.119086 0.6865533 0.1803055 PLEKHA5 Pleckstrin homology domain containing, family A member 5 566 +/- 51 398 +/- 55 952 +/- 56 599 +/- 55 0.000798 -0.048983 -0.001487 0.0944605 0.0453485 PLEKHG5 pleckstrin homology domain containing, family G (with RhoGef domain) member 5 548 +/- 58 556 +/- 54 879 +/- 69 875 +/- 97 0.000183 0.928448 -0.973622 0.7542979 0.7238556 PLIN perilipin 253 +/- 24 316 +/- 33 401 +/- 27 354 +/- 7 0.000972 0.1520385 -0.141389 0.1926004 0.1674565 PLOD2 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 200 +/- 26 179 +/- 19 274 +/- 19 272 +/- 4 0.000682 -0.526641 -0.922163 0.7050425 0.7733562 PLXNA3 plexin A3 205 +/- 22 318 +/- 36 319 +/- 36 416 +/- 24 0.017336 0.0281193 0.054836 0.0386071 0.213522 PLXNA4A plexin A4, A 1258 +/- 206 1199 +/- 232 2560 +/- 186 2273 +/- 82 3.24E-05 -0.853418 -0.202188 0.7929925 0.1445527 PMM1 phosphomannomutase 1 557 +/- 41 531 +/- 20 616 +/- 23 601 +/- 34 0.035453 -0.589295 -0.724655 0.482258 0.7162024 PMS2 PMS2 postmeiotic segregation increased 2 (S. cerevisiae) 429 +/- 49 1254 +/- 258 530 +/- 58 1843 +/- 341 0.028227 0.0231495 0.017205 0.2009082 0.1194689 PMS2L1 postmeiotic segregation increased 2-like 1 1989 +/- 162 1846 +/- 195 2743 +/- 170 2739 +/- 122 0.00052 -0.583559 -0.985026 0.3680355 0.3705689

S2-genes differentially expressed across region.xls Page 22 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI PNCK pregnancy upregulated non-ubiquitously expressed CaM kinase 583 +/- 36 459 +/- 43 769 +/- 37 629 +/- 37 0.000565 -0.052096 -0.026386 0.0213662 0.0186213 PNMA6A paraneoplastic antigen like 6A 352 +/- 18 343 +/- 34 403 +/- 30 414 +/- 16 0.047138 -0.804881 0.765792 0.4455074 0.7412188 PNPO pyridoxine 5'-phosphate oxidase 786 +/- 88 738 +/- 63 971 +/- 36 1091 +/- 52 0.000492 -0.671859 0.096909 0.5164823 0.3120607 POLD2 Polymerase (DNA directed), delta 2, regulatory subunit 50kDa 88 +/- 13 142 +/- 12 136 +/- 20 197 +/- 12 0.002295 0.0118311 0.028238 0.0796663 0.2561202 POLG polymerase (DNA directed), gamma 1237 +/- 137 1440 +/- 127 1489 +/- 88 1711 +/- 27 0.01403 0.3033735 0.053158 0.1883551 0.1166869 POLR2J2 DNA directed RNA polymerase II polypeptide J-related gene 99 +/- 12 109 +/- 12 148 +/- 7 152 +/- 18 0.000431 0.5514201 0.845704 0.5854269 0.8352996 POLR3G polymerase (RNA) III (DNA directed) polypeptide G (32kD) 105 +/- 12 145 +/- 26 185 +/- 19 196 +/- 22 0.001781 0.1969307 0.703311 0.0626024 0.8856579 POLS Polymerase (DNA directed) sigma 123 +/- 12 117 +/- 13 164 +/- 16 125 +/- 15 0.045445 -0.72565 -0.103764 0.9247623 0.1931912 PPARGC1Bperoxisome proliferative activated receptor, gamma, coactivator 1, beta 214 +/- 21 190 +/- 18 302 +/- 26 318 +/- 26 0.000445 -0.395729 0.688727 0.291459 0.6419474 PPAT phosphoribosyl pyrophosphate amidotransferase 402 +/- 24 384 +/- 26 457 +/- 19 448 +/- 9 0.021824 -0.625755 -0.711799 0.6931226 0.8975347 PPFIA2 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (lip 2104 +/- 410 1922 +/- 516 4103 +/- 315 4205 +/- 250 0.000988 -0.787347 0.806498 0.6836595 0.7915775 PPFIA4 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (lip 1441 +/- 132 1292 +/- 155 2032 +/- 145 1947 +/- 101 0.000379 -0.484273 -0.645035 0.3093163 0.6870115 PPM1E protein phosphatase 1E (PP2C domain containing) 2356 +/- 357 1782 +/- 252 2989 +/- 122 2473 +/- 54 0.006709 -0.221718 -0.0065 0.2717452 0.074406 PPM1G protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform 878 +/- 33 852 +/- 20 906 +/- 20 891 +/- 11 0.022641 -0.519842 -0.537097 0.6782777 0.8354357 PPP1CB protein phosphatase 1, catalytic subunit, beta isoform 508 +/- 37 480 +/- 29 699 +/- 42 612 +/- 54 0.000824 -0.558828 -0.237574 0.8092809 0.2882098 PPP1CC protein phosphatase 1, catalytic subunit, gamma isoform 5446 +/- 240 5140 +/- 254 5881 +/- 153 6000 +/- 183 0.001545 -0.401496 0.630623 0.3485333 0.8270337 PPP1R1A protein phosphatase 1, regulatory (inhibitor) subunit 1A 1913 +/- 322 2096 +/- 505 4210 +/- 569 4809 +/- 390 0.001982 0.7666381 0.409282 0.8644658 0.3356238 PPP1R3E Protein phosphatase 1, regulatory (inhibitor) subunit 3E 694 +/- 57 902 +/- 71 992 +/- 47 1125 +/- 45 0.000582 0.0457328 0.071171 0.0148811 0.0623085 PPP1R3F protein phosphatase 1, regulatory (inhibitor) subunit 3F 346 +/- 24 382 +/- 21 470 +/- 15 458 +/- 26 0.001123 0.2919433 -0.703384 0.429591 0.1955556 PPP1R9B protein phosphatase 1, regulatory subunit 9B, spinophilin 7112 +/- 616 6504 +/- 616 8895 +/- 337 9332 +/- 312 0.00063 -0.500824 0.366438 0.3552957 0.1971081 PPTC7 PTC7 protein phosphatase homolog (S. cerevisiae) 1273 +/- 82 1367 +/- 107 1348 +/- 44 1566 +/- 91 0.031917 0.5031911 0.074304 0.8513528 0.1415888 PR47 platelet receptor for type III collagen, 47 kDa 322 +/- 22 435 +/- 46 397 +/- 25 512 +/- 42 0.002998 0.0619977 0.054506 0.0131489 0.0095289 PRDM2 PR domain containing 2, with ZNF domain 347 +/- 45 386 +/- 34 436 +/- 22 557 +/- 23 0.001651 0.5105142 0.004008 0.5401439 0.004555 PRDM5 PR domain containing 5 120 +/- 17 108 +/- 8 149 +/- 6 142 +/- 12 0.00768 -0.539256 -0.64462 0.3091156 0.2586583 PRDM7 PR domain containing 7 105 +/- 9 95 +/- 9 145 +/- 16 145 +/- 10 0.000165 -0.448909 0.978421 0.5126945 0.8346422 PRDM8 PR domain containing 8 152 +/- 13 167 +/- 16 295 +/- 39 307 +/- 45 0.000259 0.473436 0.851306 0.7118337 0.9701559 PRDX6 peroxiredoxin 6 5355 +/- 529 6504 +/- 257 6108 +/- 398 7058 +/- 298 0.036231 0.0905172 0.089208 0.113441 0.527258 PRKACA protein kinase, cAMP-dependent, catalytic, alpha 100 +/- 12 122 +/- 12 205 +/- 16 218 +/- 20 5.43E-05 0.2280085 0.641599 0.3751015 0.6618126 PRKCE protein kinase C, epsilon 3996 +/- 353 3507 +/- 372 4337 +/- 256 4031 +/- 136 0.006434 -0.362185 -0.323772 0.4046337 0.7751352 PRKCG protein kinase C, gamma 2371 +/- 543 1678 +/- 233 3102 +/- 262 3228 +/- 306 0.012822 -0.280593 0.762009 0.1618917 0.8708546 PRMT6 protein arginine methyltransferase 6 268 +/- 33 294 +/- 30 321 +/- 41 345 +/- 27 0.009411 0.5607052 0.638768 0.605056 0.7282607 PRO0149 PRO0149 protein 3917 +/- 388 3006 +/- 191 4784 +/- 216 3768 +/- 160 0.000998 -0.071798 -0.004643 0.1491221 0.0692187 ProSAPiP1ProSAPiP1 protein 3884 +/- 236 3677 +/- 237 4142 +/- 254 4345 +/- 227 0.037457 -0.5503 0.566546 0.2422239 0.643564 PROSC proline synthetase co-transcribed homolog (bacterial) 599 +/- 88 459 +/- 83 723 +/- 106 583 +/- 146 0.034988 -0.274495 -0.461482 0.3828644 0.3152514 PROX1 Prospero-related homeobox 1 672 +/- 187 825 +/- 133 1884 +/- 163 1688 +/- 231 0.000538 0.5229816 -0.510036 0.3625898 0.872622 PRPF18 PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) 115 +/- 10 97 +/- 13 153 +/- 18 133 +/- 14 0.023639 -0.293936 -0.414367 0.4640357 0.5258124 PRPF38B PRP38 pre-mRNA processing factor 38 (yeast) domain containing B 78 +/- 7 84 +/- 11 106 +/- 10 151 +/- 25 0.010801 0.655778 0.15812 0.3464512 0.5934427 PRPS1 phosphoribosyl pyrophosphate synthetase 1 134 +/- 15 123 +/- 11 177 +/- 14 207 +/- 9 0.00147 -0.554264 0.115717 0.208459 0.7674876 PRR12 proline rich 12 521 +/- 29 456 +/- 25 874 +/- 37 813 +/- 56 6.58E-06 -0.119664 -0.401013 0.0721456 0.8684192 PRR14 proline rich 14 629 +/- 43 691 +/- 22 1014 +/- 25 946 +/- 28 7.93E-06 0.233552 -0.104254 0.2307053 0.4352825 PRR5 proline rich 5 (renal) 314 +/- 23 325 +/- 37 385 +/- 30 404 +/- 57 0.017983 0.8135638 0.776268 0.5733081 0.6633652 PRRT2 proline-rich transmembrane protein 2 2834 +/- 205 2773 +/- 306 4220 +/- 265 4039 +/- 143 4.38E-05 -0.872433 -0.565246 0.4240193 0.344864 PRSS21 protease, serine, 21 (testisin) 154 +/- 18 182 +/- 13 167 +/- 4 246 +/- 21 0.020264 0.2276938 0.019111 0.026258 2.454E-05 PRUNE prune homolog (Drosophila) 113 +/- 20 83 +/- 8 141 +/- 15 138 +/- 7 0.024337 -0.195964 -0.864036 0.2726022 0.6885329 PSAP prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) 7343 +/- 430 6944 +/- 440 7898 +/- 292 8391 +/- 294 0.002159 -0.5309 0.264842 0.2125014 0.8457605

S2-genes differentially expressed across region.xls Page 23 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI PSCD2 pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2) 1012 +/- 49 896 +/- 46 1070 +/- 31 1034 +/- 25 0.014539 -0.11572 -0.402603 0.0387049 0.153735 PSD pleckstrin and Sec7 domain containing 6439 +/- 917 5362 +/- 836 8516 +/- 437 8250 +/- 403 0.002204 -0.406092 -0.664429 0.1999838 0.4657071 PSMA1 proteasome (prosome, macropain) subunit, alpha type, 1 2881 +/- 149 2517 +/- 112 3221 +/- 146 2822 +/- 191 0.049485 -0.081981 -0.135503 0.1593462 0.6351256 PSMB7 Proteasome (prosome, macropain) subunit, beta type, 7 371 +/- 27 515 +/- 39 467 +/- 43 650 +/- 85 0.007924 0.0143763 0.103113 0.0689831 0.4534666 PSMF1 proteasome (prosome, macropain) inhibitor subunit 1 (PI31) 521 +/- 41 572 +/- 19 669 +/- 30 638 +/- 45 0.0045 0.2966879 -0.573243 0.2071042 0.4439162 PSRC1 proline/serine-rich coiled-coil 1 1655 +/- 228 1682 +/- 286 2422 +/- 176 2462 +/- 145 0.00491 0.9429163 0.86546 0.9621631 0.5532965 PTCHD2 patched domain containing 2 219 +/- 37 281 +/- 44 410 +/- 14 382 +/- 62 0.012216 0.3094278 -0.683136 0.3869426 0.9903197 PTK2B PTK2B protein tyrosine kinase 2 beta 1130 +/- 248 892 +/- 144 1495 +/- 96 1331 +/- 31 0.008662 -0.430023 -0.15465 0.2807783 0.4040677 PTMS parathymosin 564 +/- 27 451 +/- 59 772 +/- 43 611 +/- 28 0.001747 -0.12446 -0.013133 0.1036381 0.0200266 PTOV1 prostate tumor overexpressed gene 1 3925 +/- 83 4061 +/- 73 4615 +/- 175 5076 +/- 123 0.000138 0.2433193 0.060998 0.0969817 0.2466308 PTPLA protein tyrosine phosphatase-like (proline instead of catalytic arginine), member A 173 +/- 49 187 +/- 26 358 +/- 29 307 +/- 36 5.84E-05 0.81363 -0.304362 0.5596936 0.5725918 PTPLAD1 protein tyrosine phosphatase-like A domain containing 1 6112 +/- 562 7457 +/- 399 7287 +/- 65 8263 +/- 403 0.015102 0.0830088 0.071859 0.2210998 0.188772 PTPN2 protein tyrosine phosphatase, non-receptor type 2 179 +/- 25 130 +/- 5 268 +/- 19 220 +/- 30 0.001237 -0.10636 -0.22009 0.1014129 0.3462098 PTPN23 protein tyrosine phosphatase, non-receptor type 23 151 +/- 10 158 +/- 7 380 +/- 24 437 +/- 34 6.44E-07 0.5989845 0.219274 0.9075274 0.3110285 PTPN4 protein tyrosine phosphatase, non-receptor type 4 (megakaryocyte) 246 +/- 44 202 +/- 24 319 +/- 38 235 +/- 20 0.030546 -0.405015 -0.085597 0.4106939 0.1783452 PTPRS protein tyrosine phosphatase, receptor type, S 82 +/- 20 72 +/- 18 131 +/- 10 126 +/- 15 0.004117 -0.719537 -0.797785 0.9997926 0.7536546 PTS 6-pyruvoyltetrahydropterin synthase 385 +/- 18 425 +/- 33 533 +/- 49 536 +/- 60 0.000163 0.3145322 0.975155 0.4432393 0.911116 PTTG1IP pituitary tumor-transforming 1 interacting protein 1120 +/- 55 1138 +/- 62 1299 +/- 51 1386 +/- 77 0.017836 0.823153 0.375653 0.3940951 0.8723619 PURB purine-rich element binding protein B 936 +/- 78 839 +/- 60 1218 +/- 51 1114 +/- 68 0.001017 -0.347596 -0.259331 0.1063926 0.0474873 PUS7 pseudouridylate synthase 7 homolog (S. cerevisiae) 228 +/- 10 224 +/- 36 248 +/- 12 252 +/- 46 0.021269 -0.916403 0.934618 0.3232159 0.1708657 PVR poliovirus receptor 87 +/- 11 110 +/- 6 130 +/- 6 144 +/- 14 0.003259 0.0942913 0.390507 0.0707403 0.1747505 QPCT glutaminyl-peptide cyclotransferase (glutaminyl cyclase) 527 +/- 102 510 +/- 108 984 +/- 86 1096 +/- 204 2.22E-05 -0.912268 0.633141 0.8001084 0.690394 QSCN6L1 quiescin Q6-like 1 715 +/- 62 579 +/- 58 1381 +/- 87 1148 +/- 33 1.21E-09 -0.143148 -0.044212 0.175206 0.1498206 QTRT1 queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) 256 +/- 22 498 +/- 21 323 +/- 31 587 +/- 36 0.020811 1.482E-05 0.000476 0.0059661 0.0016492 RAB10 RAB10, member RAS oncogene family 105 +/- 12 136 +/- 19 144 +/- 11 143 +/- 8 0.039699 0.2097026 -0.918551 0.1844926 0.3004474 RAB11B RAB11B, member RAS oncogene family 4485 +/- 192 4565 +/- 280 6708 +/- 245 6448 +/- 94 3.62E-07 0.8183857 -0.358544 0.855232 0.4698891 RAB11FIP2RAB11 family interacting protein 2 (class I) 804 +/- 77 838 +/- 90 1269 +/- 118 1219 +/- 37 9.71E-05 0.7825742 -0.700985 0.8370775 0.748893 RAB12 RAB12, member RAS oncogene family 321 +/- 25 439 +/- 34 442 +/- 78 498 +/- 58 0.034932 0.0211394 0.581549 0.0528651 0.6551118 RAB22A RAB22A, member RAS oncogene family 3247 +/- 178 2973 +/- 123 3675 +/- 96 3565 +/- 162 0.001043 -0.238385 -0.577928 0.1773529 0.5680772 RAB39B RAB39B, member RAS oncogene family 898 +/- 51 860 +/- 25 1029 +/- 72 1040 +/- 31 0.004883 -0.520088 0.893186 0.2007007 0.9953222 RAB3D RAB3D, member RAS oncogene family 194 +/- 32 175 +/- 17 257 +/- 27 288 +/- 16 0.001971 -0.602833 0.354208 0.8057355 0.2833045 RAB3IP RAB3A interacting protein (rabin3) 167 +/- 12 191 +/- 19 202 +/- 19 246 +/- 15 0.00961 0.3068411 0.097491 0.2146503 0.361122 RAB6IP2 RAB6 interacting protein 2 153 +/- 26 103 +/- 9 195 +/- 25 180 +/- 21 0.015009 -0.120469 -0.665416 0.0685205 0.7651142 RAB8B RAB8B, member RAS oncogene family 397 +/- 38 402 +/- 36 545 +/- 46 601 +/- 43 0.001902 0.9257873 0.402756 0.9600861 0.8778103 RABEPK Rab9 effector protein with kelch motifs 1221 +/- 139 1124 +/- 116 1788 +/- 162 1733 +/- 114 0.000954 -0.604913 -0.788298 0.3888761 0.4776052 RAC3 ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) 489 +/- 32 422 +/- 12 573 +/- 36 591 +/- 29 0.004033 -0.099674 0.703528 0.0434275 0.7173997 RAD23A RAD23 homolog A (S. cerevisiae) 2113 +/- 100 2174 +/- 122 2527 +/- 127 2568 +/- 209 0.005817 0.7071364 0.870964 0.9465637 0.3990426 RAD51L3 RAD51-like 3 (S. cerevisiae) 190 +/- 9 204 +/- 12 250 +/- 15 240 +/- 10 0.004866 0.3655233 -0.592161 0.2344551 0.7811717 RAD52 RAD52 homolog (S. cerevisiae) 114 +/- 21 165 +/- 13 189 +/- 16 213 +/- 25 0.0174 0.0744451 0.449711 0.0124654 0.6361856 RALGPS2 Ral GEF with PH domain and SH3 binding motif 2 280 +/- 27 193 +/- 19 326 +/- 37 295 +/- 16 0.008765 -0.026246 -0.484099 0.2424974 0.6605985 RANBP5 RAN binding protein 5 936 +/- 51 1233 +/- 94 1110 +/- 54 1359 +/- 44 0.039116 0.025319 0.005956 0.1892396 0.1865095 RANBP9 RAN binding protein 9 690 +/- 55 605 +/- 43 849 +/- 24 906 +/- 80 0.002602 -0.253063 0.527108 0.2836185 0.9596956 RAPGEF1 Rap guanine nucleotide exchange factor (GEF) 1 616 +/- 50 396 +/- 101 775 +/- 26 416 +/- 82 0.043348 -0.091584 -0.0095 0.072241 0.0039693 RAPGEF2 Rap guanine nucleotide exchange factor (GEF) 2 83 +/- 10 62 +/- 10 145 +/- 12 119 +/- 4 2.34E-05 -0.179458 -0.088938 0.1507976 0.037781 RAPGEFL1Rap guanine nucleotide exchange factor (GEF)-like 1 1885 +/- 109 1723 +/- 162 2860 +/- 200 2567 +/- 150 6.42E-05 -0.427791 -0.271608 0.3111031 0.5439641

S2-genes differentially expressed across region.xls Page 24 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI RAPH1 Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 176 +/- 32 166 +/- 25 251 +/- 33 244 +/- 29 0.030404 -0.797325 -0.860131 0.6091869 0.6624251 RASA1 RAS p21 protein activator (GTPase activating protein) 1 280 +/- 29 303 +/- 28 361 +/- 33 358 +/- 48 0.034413 0.5864133 -0.957737 0.1111604 0.5111607 RASA2 RAS p21 protein activator 2 418 +/- 42 488 +/- 35 603 +/- 51 671 +/- 18 0.000205 0.2347056 0.252934 0.4320581 0.5140061 RASA3 RAS p21 protein activator 3 162 +/- 27 166 +/- 20 200 +/- 15 257 +/- 20 0.008713 0.9114708 0.052284 0.8869965 0.0237445 RASAL1 RAS protein activator like 1 (GAP1 like) 314 +/- 37 235 +/- 36 343 +/- 28 328 +/- 17 0.047983 -0.153951 -0.670509 0.2264319 0.5461943 RASAL2 RAS protein activator like 2 324 +/- 18 409 +/- 46 449 +/- 47 452 +/- 65 0.008586 0.1290618 0.972509 0.0276984 0.5289273 RASD2 RASD family, member 2 813 +/- 162 773 +/- 176 1607 +/- 232 1677 +/- 147 0.000628 -0.872824 0.806836 0.6943011 0.6564199 RASGRF2 Ras protein-specific guanine nucleotide-releasing factor 2 335 +/- 22 273 +/- 17 423 +/- 53 365 +/- 12 0.015254 -0.048142 -0.333609 0.0328393 0.3533591 RASL10B RAS-like, family 10, member B 590 +/- 59 624 +/- 87 801 +/- 45 879 +/- 127 0.001014 0.7507346 0.588747 0.98104 0.8326617 RASL11A RAS-like, family 11, member A 132 +/- 22 136 +/- 9 169 +/- 20 178 +/- 23 0.039437 0.8723694 0.788188 0.7101726 0.6581739 RBBP4 Retinoblastoma binding protein 4 249 +/- 20 207 +/- 24 276 +/- 21 291 +/- 29 0.006744 -0.19939 0.685426 0.1893722 0.4350101 RBM11 RNA binding motif protein 11 201 +/- 38 195 +/- 16 284 +/- 42 306 +/- 16 0.001256 -0.884347 0.644036 0.9732285 0.7444476 RBM13 RNA binding motif protein 13 463 +/- 34 385 +/- 16 506 +/- 17 477 +/- 18 0.009931 -0.077644 -0.271747 0.1756137 0.9210227 RBM15B RNA binding motif protein 15B 878 +/- 30 852 +/- 59 943 +/- 44 973 +/- 49 0.039279 -0.704646 0.658372 0.7465815 0.5583219 RBM25 RNA binding motif protein 25 1188 +/- 33 1453 +/- 33 1540 +/- 42 1830 +/- 101 3.38E-05 0.0002076 0.041769 0.0052 0.2262857 RBM33 RNA binding motif protein 33 475 +/- 19 501 +/- 6 548 +/- 20 591 +/- 30 0.001125 0.2382658 0.271446 0.1788918 0.2214815 RBMXL1 RNA binding motif protein, X-linked-like 1 1447 +/- 44 1539 +/- 65 1762 +/- 51 1747 +/- 55 0.000596 0.2720333 -0.851126 0.2747617 0.9421726 RBP4 retinol binding protein 4, plasma 3170 +/- 320 2513 +/- 318 3602 +/- 191 3351 +/- 160 0.021238 -0.175499 -0.341143 0.1610624 0.1462001 RCE1 RCE1 homolog, prenyl protein peptidase (S. cerevisiae) 601 +/- 19 662 +/- 33 808 +/- 58 687 +/- 35 0.016929 0.1446353 -0.108276 0.1947384 0.2062164 RCOR1 REST corepressor 1 724 +/- 69 692 +/- 65 959 +/- 33 890 +/- 52 0.004938 -0.740265 -0.305182 0.6085367 0.8458175 RDH10 retinol dehydrogenase 10 (all-trans) 114 +/- 20 92 +/- 17 135 +/- 14 145 +/- 26 0.015836 -0.419978 0.725073 0.719916 0.8607565 RDH11 retinol dehydrogenase 11 (all-trans/9-cis/11-cis) 849 +/- 27 901 +/- 60 1459 +/- 71 1744 +/- 114 9.86E-07 0.4576926 0.073114 0.4798064 0.354908 REEP2 receptor accessory protein 2 1273 +/- 34 1129 +/- 71 1401 +/- 45 1248 +/- 55 0.000165 -0.108961 -0.061383 0.1025827 0.0758981 RENBP renin binding protein 106 +/- 14 148 +/- 12 134 +/- 15 152 +/- 8 0.018497 0.053118 0.33391 0.1560433 0.2784616 REXO1 REX1, RNA exonuclease 1 homolog (S. cerevisiae) 655 +/- 28 517 +/- 30 915 +/- 58 744 +/- 67 0.000389 -0.007209 -0.08753 0.0019855 0.2431326 RFNG radical fringe homolog (Drosophila) 490 +/- 18 545 +/- 21 583 +/- 19 626 +/- 14 0.000199 0.0700264 0.109561 0.1126252 0.41884 RFP2 ret finger protein 2 851 +/- 43 760 +/- 39 942 +/- 65 860 +/- 22 0.014094 -0.153933 -0.27531 0.4538743 0.7489593 RFXDC2 regulatory factor X domain containing 2 118 +/- 18 107 +/- 10 152 +/- 16 146 +/- 13 0.036934 -0.615093 -0.770932 0.8637923 0.7002586 RGAG4 retrotransposon gag domain containing 4 755 +/- 18 741 +/- 51 846 +/- 36 768 +/- 36 0.035734 -0.805065 -0.157981 0.8526463 0.178816 RGMB RGM domain family, member B 819 +/- 119 647 +/- 111 1010 +/- 93 865 +/- 99 0.000703 -0.315691 -0.315332 0.1146151 0.1019076 RGPD5 RANBP2-like and GRIP domain containing 5 241 +/- 37 184 +/- 13 312 +/- 43 248 +/- 22 0.019929 -0.194614 -0.220043 0.2570417 0.5634338 RGS11 regulator of G-protein signalling 11 262 +/- 54 525 +/- 62 397 +/- 50 606 +/- 54 0.016368 0.0094108 0.019498 0.0761906 0.0269708 RHOT1 ras homolog gene family, member T1 273 +/- 25 323 +/- 28 377 +/- 19 343 +/- 13 0.006699 0.2147774 -0.164211 0.2595788 0.1355325 RIC3 resistance to inhibitors of cholinesterase 3 homolog (C. elegans) 250 +/- 42 198 +/- 12 287 +/- 31 316 +/- 23 0.037902 -0.281804 0.46362 0.1732732 0.8237914 RICS Rho GTPase-activating protein 284 +/- 32 258 +/- 18 351 +/- 20 392 +/- 24 0.004588 -0.500555 0.218109 0.3805116 0.1281806 RIS1 Ras-induced senescence 1 1904 +/- 153 2190 +/- 193 2560 +/- 118 2356 +/- 92 0.013539 0.2718364 -0.207118 0.7430148 0.1753443 RIT2 Ras-like without CAAX 2 609 +/- 31 437 +/- 55 775 +/- 62 568 +/- 48 0.002842 -0.025575 -0.027876 0.0778629 0.0628945 RLTPR RGD, leucine-rich repeat, tropomodulin and proline-rich containing protein 1022 +/- 127 895 +/- 85 1350 +/- 79 1389 +/- 68 0.001896 -0.429193 0.713318 0.4223463 0.7380079 RMND5A required for meiotic nuclear division 5 homolog A (S. cerevisiae) 1839 +/- 181 1737 +/- 125 2391 +/- 146 2367 +/- 191 0.000845 -0.653898 -0.923399 0.9939108 0.8336583 RMND5B required for meiotic nuclear division 5 homolog B (S. cerevisiae) 479 +/- 38 527 +/- 42 584 +/- 33 601 +/- 17 0.006951 0.4215034 0.657046 0.7190742 0.8324462 RNASEH1 ribonuclease H1 129 +/- 15 178 +/- 14 148 +/- 7 241 +/- 18 0.013749 0.0416065 0.004633 0.229431 0.0325456 RNF123 ring finger protein 123 577 +/- 21 600 +/- 34 633 +/- 43 704 +/- 35 0.03273 0.5805279 0.232506 0.9420261 0.2414312 RNF170 ring finger protein 170 384 +/- 39 453 +/- 32 518 +/- 55 493 +/- 32 0.023423 0.1997535 -0.698505 0.1197989 0.7497304 RNF182 ring finger protein 182 1190 +/- 126 1299 +/- 67 1679 +/- 123 1573 +/- 80 8.75E-06 0.4644971 -0.4904 0.1707664 0.6235059 RNF19 ring finger protein 19 293 +/- 27 343 +/- 26 377 +/- 19 413 +/- 31 0.002022 0.2099337 0.357874 0.0553089 0.0604769

S2-genes differentially expressed across region.xls Page 25 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI RNF24 ring finger protein 24 1650 +/- 211 1457 +/- 243 2321 +/- 131 2201 +/- 160 1.03E-05 -0.562966 -0.574743 0.3200983 0.384435 RNF40 ring finger protein 40 697 +/- 31 616 +/- 10 876 +/- 27 750 +/- 31 0.000102 -0.043587 -0.014865 0.3401724 0.1080425 RNF41 ring finger protein 41 827 +/- 108 700 +/- 95 920 +/- 42 898 +/- 97 0.038621 -0.398743 -0.845613 0.3846319 0.7096138 RNF7 ring finger protein 7 1784 +/- 108 1655 +/- 68 1864 +/- 122 1940 +/- 81 0.008816 -0.341892 0.615917 0.2100194 0.973737 RNMT RNA (guanine-7-) methyltransferase 171 +/- 23 268 +/- 68 232 +/- 22 332 +/- 75 0.001433 0.2213002 0.25954 0.2409138 0.1839788 RNPC2 RNA-binding region (RNP1, RRM) containing 2 2981 +/- 203 2776 +/- 143 3467 +/- 85 3303 +/- 105 0.001494 -0.430685 -0.258494 0.6252206 0.383015 RNPS1 RNA binding protein S1, serine-rich domain 2748 +/- 78 3124 +/- 133 3034 +/- 149 3248 +/- 132 0.026594 0.0408598 0.310943 0.2161541 0.4479756 ROBO1 Roundabout, axon guidance receptor, homolog 1 (Drosophila) 342 +/- 33 264 +/- 31 489 +/- 6 390 +/- 40 0.000387 -0.110332 -0.06635 0.3312246 0.603104 ROD1 ROD1 regulator of differentiation 1 (S. pombe) 1784 +/- 98 1541 +/- 71 1943 +/- 113 1743 +/- 34 0.011037 -0.074084 -0.142457 0.0463187 0.1875405 ROGDI rogdi homolog (Drosophila) 4108 +/- 229 4655 +/- 269 5282 +/- 353 5333 +/- 277 4.61E-05 0.1530418 0.91117 0.1079876 0.8690097 ROR1 Receptor tyrosine kinase-like orphan receptor 1 124 +/- 55 109 +/- 33 385 +/- 44 336 +/- 30 0.000969 -0.81109 -0.385332 0.8684625 0.3481164 RP6-213H1Mst3 and SOK1-related kinase 140 +/- 24 156 +/- 39 261 +/- 60 281 +/- 49 0.01556 0.7356112 0.799393 0.9374917 0.8537854 RPESP RPE-spondin 216 +/- 36 219 +/- 54 370 +/- 37 509 +/- 43 0.001557 0.9565057 0.036551 0.7215285 0.0174944 RPIB9 Rap2-binding protein 9 1388 +/- 111 1270 +/- 94 1665 +/- 42 1512 +/- 53 0.005251 -0.437014 -0.054718 0.4532659 0.3512872 RPIP8 RaP2 interacting protein 8 3549 +/- 231 3204 +/- 200 3873 +/- 200 4026 +/- 156 0.010044 -0.286464 0.56282 0.16043 0.6201375 RPL31 ribosomal protein L31 232 +/- 31 172 +/- 17 275 +/- 9 198 +/- 25 0.048221 -0.128385 -0.032936 0.3364343 0.2694518 RPRC1 arginine/proline rich coiled-coil 1 6598 +/- 221 6480 +/- 243 6871 +/- 111 7286 +/- 69 0.036021 -0.725681 0.013169 0.5078475 0.1042715 RRAGD Ras-related GTP binding D 2145 +/- 150 1853 +/- 133 2546 +/- 120 2476 +/- 88 0.003177 -0.177376 -0.647677 0.1387147 0.5482913 RRH retinal pigment epithelium-derived rhodopsin homolog 138 +/- 8 123 +/- 14 177 +/- 19 150 +/- 4 0.008474 -0.36222 -0.203525 0.3094094 0.4788856 RSAD1 radical S-adenosyl methionine domain containing 1 480 +/- 57 589 +/- 47 652 +/- 40 794 +/- 59 0.001206 0.1749976 0.084648 0.4121308 0.3750654 RSC1A1 regulatory solute carrier protein, family 1, member 1 800 +/- 32 890 +/- 37 1000 +/- 54 1029 +/- 56 0.002255 0.0946429 0.720803 0.0176162 0.3233742 RTEL1 regulator of telomere elongation helicase 1 121 +/- 22 88 +/- 9 196 +/- 27 159 +/- 22 0.000236 -0.209402 -0.314121 0.4474691 0.7839512 RTF1 Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) 1080 +/- 61 889 +/- 52 1157 +/- 39 1177 +/- 28 0.006301 -0.039212 0.687224 0.0166795 0.9882032 RTN4IP1 reticulon 4 interacting protein 1 154 +/- 6 130 +/- 11 186 +/- 18 167 +/- 25 0.033602 -0.101709 -0.537449 0.1266909 0.550043 RTN4RL2 reticulon 4 receptor-like 2 163 +/- 33 113 +/- 19 235 +/- 8 213 +/- 18 0.003015 -0.220541 -0.317217 0.1607859 0.1478692 RUFY2 RUN and FYVE domain containing 2 706 +/- 76 907 +/- 57 1027 +/- 29 1122 +/- 40 0.000576 0.0633193 0.09295 0.0648253 0.0346075 RUFY3 RUN and FYVE domain containing 3 69 +/- 10 111 +/- 9 114 +/- 13 156 +/- 17 0.001984 0.0099158 0.096064 0.0138411 0.0161513 RUTBC1 RUN and TBC1 domain containing 1 206 +/- 31 241 +/- 29 415 +/- 35 402 +/- 20 0.000147 0.4340995 -0.758055 0.4950478 0.6240771 RXRG retinoid X receptor, gamma 219 +/- 45 249 +/- 28 413 +/- 47 365 +/- 26 7.42E-05 0.5891601 -0.396336 0.6832858 0.4130308 SAC3D1 SAC3 domain containing 1 657 +/- 52 643 +/- 23 778 +/- 30 788 +/- 31 0.005319 -0.817125 0.82524 0.9655861 0.6202256 SAFB2 scaffold attachment factor B2 1217 +/- 84 1373 +/- 83 1547 +/- 101 1630 +/- 96 0.004611 0.2123291 0.56266 0.2630684 0.4842056 SALL3 sal-like 3 (Drosophila) 194 +/- 13 178 +/- 21 249 +/- 17 210 +/- 26 0.000545 -0.543745 -0.251428 0.2743355 0.0482473 SAMD1 sterile alpha motif domain containing 1 363 +/- 22 312 +/- 35 561 +/- 23 439 +/- 31 2.83E-07 -0.249772 -0.013804 0.2150173 0.0153949 SAMD13 sterile alpha motif domain containing 13 234 +/- 23 238 +/- 21 261 +/- 20 314 +/- 8 0.027004 0.9033007 0.044956 0.9222472 0.2310658 SAP30L SAP30-like 230 +/- 15 253 +/- 20 262 +/- 5 279 +/- 19 0.04287 0.4003901 0.418652 0.1061515 0.0425077 SAPS1 SAPS domain family, member 1 1077 +/- 65 1094 +/- 119 1530 +/- 101 1540 +/- 59 0.000181 0.9038836 0.938047 0.7853608 0.8280362 SAS10 disrupter of silencing 10 741 +/- 61 612 +/- 20 813 +/- 19 717 +/- 46 0.026176 -0.090135 -0.109822 0.0878673 0.0545552 SBF2 SET binding factor 2 129 +/- 20 128 +/- 22 180 +/- 15 156 +/- 18 0.000919 -0.985719 -0.31673 0.8668356 0.9668462 SBNO1 Sno, strawberry notch homolog 1 (Drosophila) 160 +/- 25 122 +/- 25 232 +/- 40 181 +/- 35 0.003463 -0.303871 -0.361305 0.4112182 0.4143627 SCAMP4 secretory carrier membrane protein 4 743 +/- 44 842 +/- 65 875 +/- 23 973 +/- 45 0.002716 0.2408748 0.098777 0.414176 0.3994015 SCAMP5 secretory carrier membrane protein 5 7112 +/- 255 6561 +/- 419 7973 +/- 275 8158 +/- 266 7.52E-05 -0.29298 0.640297 0.0321993 0.8316471 SCARNA9 Small Cajal body-specific RNA 9 162 +/- 16 184 +/- 16 196 +/- 4 209 +/- 24 0.008919 0.3537661 0.614271 0.7804901 0.9937399 SCC-112 SCC-112 protein 108 +/- 16 158 +/- 17 157 +/- 25 200 +/- 16 4.32E-05 0.0567059 0.184584 0.2494883 0.6263184 SCG3 secretogranin III 3061 +/- 250 2848 +/- 214 3541 +/- 106 3310 +/- 145 0.03373 -0.532236 -0.23503 0.4220333 0.6622037 SCHIP1 schwannomin interacting protein 1 6881 +/- 187 6571 +/- 171 7488 +/- 294 7302 +/- 360 0.021994 -0.248516 -0.699557 0.9727114 0.4526198

S2-genes differentially expressed across region.xls Page 26 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI SCN1B sodium channel, voltage-gated, type I, beta 2478 +/- 96 2640 +/- 159 3251 +/- 193 3267 +/- 114 6.85E-05 0.409883 0.942946 0.5323587 0.9471917 SCN2A2 sodium channel, voltage-gated, type II, alpha 2 8450 +/- 471 7294 +/- 521 9209 +/- 438 8616 +/- 254 0.040612 -0.131204 -0.275937 0.0441534 0.8125718 SCN8A sodium channel, voltage gated, type VIII, alpha 103 +/- 23 90 +/- 16 139 +/- 21 164 +/- 13 0.020038 -0.65351 0.35844 0.4338307 0.5295296 SCRT1 scratch homolog 1, zinc finger protein (Drosophila) 88 +/- 8 99 +/- 12 158 +/- 16 169 +/- 14 0.000196 0.4553579 0.639665 0.6343513 0.5550117 SCYL1BP1SCY1-like 1 binding protein 1 559 +/- 81 610 +/- 81 850 +/- 66 797 +/- 29 0.007999 0.6639639 -0.486864 0.8315539 0.3998438 SCYL2 SCY1-like 2 (S. cerevisiae) 1488 +/- 95 1321 +/- 44 1638 +/- 109 1535 +/- 60 0.02375 -0.153763 -0.433654 0.1074676 0.3250074 SDC2 syndecan 2 (heparan sulfate proteoglycan 1, cell surface-associated, fibroglycan) 504 +/- 26 546 +/- 65 821 +/- 58 807 +/- 31 0.000454 0.5681716 -0.84154 0.7228506 0.9073002 SDCBP syndecan binding protein (syntenin) 8109 +/- 578 6955 +/- 227 9638 +/- 508 9175 +/- 303 6.17E-05 -0.10881 -0.456545 0.0481946 0.5684862 SDCBP2 syndecan binding protein (syntenin) 2 165 +/- 43 139 +/- 25 243 +/- 36 220 +/- 24 0.00035 -0.609193 -0.611958 0.5407396 0.4125482 SDCCAG1 serologically defined colon cancer antigen 1 388 +/- 20 315 +/- 15 516 +/- 63 441 +/- 28 0.005473 -0.017003 -0.314805 0.0037034 0.1962015 SEC22A SEC22 vesicle trafficking protein homolog A (S. cerevisiae) 388 +/- 11 342 +/- 16 440 +/- 19 453 +/- 44 0.00208 -0.03712 0.804564 0.1895016 0.1610125 SEC23A Sec23 homolog A (S. cerevisiae) 459 +/- 49 359 +/- 21 572 +/- 52 577 +/- 46 0.007078 -0.101159 0.947131 0.065943 0.6801096 SEC63 SEC63-like (S. cerevisiae) 192 +/- 34 126 +/- 32 277 +/- 24 221 +/- 34 0.010326 -0.191457 -0.216541 0.2569293 0.397006 SEMA3B sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 215 +/- 39 327 +/- 34 260 +/- 19 343 +/- 40 0.031486 0.0586599 0.113443 0.1486362 0.2385468 SEMA6B sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B 2141 +/- 281 1472 +/- 204 2572 +/- 104 2191 +/- 53 0.002351 -0.085956 -0.013093 0.1058834 0.195481 SEPHS1 selenophosphate synthetase 1 297 +/- 33 289 +/- 25 383 +/- 23 377 +/- 28 0.006151 -0.845896 -0.879328 0.9433685 0.9045879 SEPT3 septin 3 7220 +/- 468 5490 +/- 263 8056 +/- 320 7357 +/- 258 0.000891 -0.012451 -0.1241 0.0213601 0.4560522 SERBP1 SERPINE1 mRNA binding protein 1 681 +/- 62 812 +/- 90 888 +/- 80 925 +/- 98 0.018134 0.260052 0.774907 0.2594121 0.4099514 SERPINB6 serpin peptidase inhibitor, clade B (ovalbumin), member 6 139 +/- 16 197 +/- 19 232 +/- 20 249 +/- 10 0.003543 0.0405549 0.469389 0.2040456 0.7728342 SERTAD2 SERTA domain containing 2 361 +/- 29 373 +/- 27 472 +/- 16 447 +/- 23 0.001359 0.7650236 -0.414877 0.4638359 0.4515351 SET SET translocation (myeloid leukemia-associated) 698 +/- 22 583 +/- 14 1015 +/- 52 866 +/- 35 2.46E-07 -0.002193 -0.04242 0.020561 0.0522707 SETD2 SET domain containing 2 77 +/- 9 85 +/- 12 129 +/- 22 118 +/- 12 0.032902 0.597282 -0.674125 0.3016181 0.6631157 SEZ6L2 Seizure related 6 homolog (mouse)-like 2 1565 +/- 18 1244 +/- 115 2230 +/- 72 2186 +/- 114 0.000161 -0.038027 -0.756044 0.0439181 0.9303992 SF1 splicing factor 1 140 +/- 11 201 +/- 16 207 +/- 11 256 +/- 26 0.000508 0.01371 0.143512 0.0146115 0.103229 SF3A1 splicing factor 3a, subunit 1, 120kDa 1775 +/- 85 1593 +/- 29 1910 +/- 46 1838 +/- 72 0.006802 -0.087211 -0.420864 0.119543 0.9283587 SF3A2 Splicing factor 3a, subunit 2, 66kDa 332 +/- 27 363 +/- 24 492 +/- 35 438 +/- 27 0.001428 0.4060509 -0.248432 0.6079222 0.5013208 SFPQ splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated) 2328 +/- 109 2456 +/- 58 2770 +/- 74 2832 +/- 78 0.000208 0.3319386 0.578521 0.2694177 0.7807726 SFRS14 splicing factor, arginine/serine-rich 14 2501 +/- 104 2096 +/- 137 2785 +/- 51 2631 +/- 85 0.00293 -0.04162 -0.167002 0.0062501 0.0353494 SFRS2 splicing factor, arginine/serine-rich 2 255 +/- 34 335 +/- 13 324 +/- 26 380 +/- 21 0.002841 0.069861 0.133479 0.0253347 0.1884405 SFRS4 splicing factor, arginine/serine-rich 4 2735 +/- 61 2832 +/- 42 2933 +/- 52 2991 +/- 82 0.030369 0.2220573 0.569278 0.1905043 0.8098299 SFXN4 sideroflexin 4 342 +/- 24 319 +/- 11 365 +/- 11 379 +/- 28 0.037644 -0.418334 0.657969 0.5080271 0.7719076 SFXN5 Sideroflexin 5 157 +/- 8 208 +/- 14 190 +/- 13 226 +/- 9 0.010934 0.0109709 0.05192 0.0002757 0.0306315 SGCB sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) 220 +/- 12 203 +/- 11 252 +/- 21 222 +/- 22 0.037749 -0.329488 -0.36182 0.1779702 0.2191165 SGIP1 SH3-domain GRB2-like (endophilin) interacting protein 1 2156 +/- 211 1772 +/- 114 2457 +/- 112 2168 +/- 82 0.001989 -0.150026 -0.068561 0.1536249 0.0617374 SH2B SH2-B homolog 1071 +/- 20 1347 +/- 59 1612 +/- 117 1439 +/- 90 0.013408 0.0041889 -0.272413 0.046181 0.3927911 SH2D5 SH2 domain containing 5 596 +/- 74 690 +/- 40 819 +/- 19 878 +/- 49 0.002288 0.2932819 0.313419 0.815493 0.6624593 SH3BGRL SH3 domain binding glutamic acid-rich protein like 1959 +/- 216 2188 +/- 169 2416 +/- 113 2842 +/- 144 0.014296 0.4222461 0.048322 0.3057342 0.2728617 SH3BGRL2SH3 domain binding glutamic acid-rich protein like 2 1572 +/- 143 1638 +/- 177 1882 +/- 178 1811 +/- 170 0.037863 0.778515 -0.779388 0.4765014 0.455684 SH3GL1 SH3-domain GRB2-like 1 517 +/- 28 419 +/- 30 649 +/- 35 550 +/- 32 0.000487 -0.037857 -0.069011 0.062626 0.4344739 SH3GLB2 SH3-domain GRB2-like endophilin B2 1534 +/- 197 1752 +/- 251 2334 +/- 168 2780 +/- 116 0.000801 0.5112276 0.058696 0.9239354 0.4527989 SH3PXD2ASH3 and PX domains 2A 408 +/- 23 519 +/- 49 572 +/- 22 578 +/- 46 0.018692 0.0816228 0.914945 0.0456051 0.3901388 SHANK1 SH3 and multiple ankyrin repeat domains 1 186 +/- 19 155 +/- 7 208 +/- 15 185 +/- 20 0.048705 -0.1764 -0.371794 0.0686279 0.3554657 SIDT1 SID1 transmembrane family, member 1 745 +/- 120 796 +/- 82 1543 +/- 51 1669 +/- 181 4.34E-05 0.7309273 0.534895 0.8091259 0.8948326 SIRT2 Sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) 1243 +/- 145 1521 +/- 75 1590 +/- 87 1721 +/- 146 0.011069 0.130881 0.469834 0.2097638 0.3628542 SKIL SKI-like 118 +/- 14 143 +/- 10 155 +/- 9 163 +/- 23 0.032871 0.1791027 0.777989 0.0542374 0.1069061

S2-genes differentially expressed across region.xls Page 27 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI SLC16A14 solute carrier family 16 (monocarboxylic acid transporters), member 14 1075 +/- 41 1004 +/- 75 1195 +/- 34 1038 +/- 52 0.036333 -0.435972 -0.03911 0.4885692 0.0290485 SLC16A4 Solute carrier family 16 (monocarboxylic acid transporters), member 4 359 +/- 31 331 +/- 40 433 +/- 20 387 +/- 34 0.044918 -0.596445 -0.296733 0.9986879 0.6256058 SLC17A7 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 20947 +/- 1525 19370 +/- 1552 21896 +/- 745 22824 +/- 1012 0.035327 -0.485189 0.482079 0.2697982 0.9226882 SLC19A2 Solute carrier family 19 (thiamine transporter), member 2 174 +/- 35 226 +/- 16 240 +/- 47 295 +/- 24 0.003342 0.2128254 0.332039 0.2491747 0.3336887 SLC1A1 solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag) 511 +/- 80 386 +/- 60 657 +/- 64 673 +/- 55 0.005747 -0.239542 0.857943 0.194656 0.9149181 SLC1A6 solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 685 +/- 50 598 +/- 48 942 +/- 40 974 +/- 44 1.11E-06 -0.237822 0.612922 0.1094085 0.7801005 SLC24A4 solute carrier family 24 (sodium/potassium/calcium exchanger), member 4 200 +/- 40 153 +/- 20 388 +/- 93 279 +/- 55 0.049151 -0.328575 -0.344011 0.4826206 0.6022908 SLC24A5 Solute carrier family 24, member 5 1183 +/- 81 1115 +/- 57 1455 +/- 56 1211 +/- 48 0.028959 -0.514493 -0.008949 0.3598755 0.0032771 SLC25A16 solute carrier family 25 (mitochondrial carrier; Graves disease autoantigen), member 16 472 +/- 27 547 +/- 23 566 +/- 28 661 +/- 17 0.001135 0.0629683 0.021427 0.4766455 0.0789001 SLC25A22 solute carrier family 25 (mitochondrial carrier: glutamate), member 22 3663 +/- 121 3709 +/- 334 5093 +/- 275 5807 +/- 450 4.23E-05 0.9003307 0.219003 0.4577215 0.6732024 SLC25A25 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 1121 +/- 61 974 +/- 52 1300 +/- 67 1101 +/- 43 0.028599 -0.096748 -0.036721 0.0306786 0.1145057 SLC25A30 solute carrier family 25, member 30 159 +/- 9 164 +/- 29 256 +/- 26 219 +/- 27 0.016928 0.8833456 -0.355562 0.8229475 0.3991031 SLC25A37 solute carrier family 25, member 37 1037 +/- 82 1243 +/- 98 1265 +/- 119 1375 +/- 54 0.007367 0.1387508 0.427593 0.0715431 0.3137814 SLC26A10 solute carrier family 26, member 10 99 +/- 20 85 +/- 13 132 +/- 17 114 +/- 12 0.012561 -0.587428 -0.426805 0.3089693 0.2860397 SLC2A11 solute carrier family 2 (facilitated glucose transporter), member 11 337 +/- 24 347 +/- 19 470 +/- 21 450 +/- 14 3.6E-05 0.7394265 -0.461701 0.9635415 0.5019672 SLC2A13 solute carrier family 2 (facilitated glucose transporter), member 13 2788 +/- 251 2900 +/- 266 4267 +/- 144 4652 +/- 260 5.35E-05 0.7649466 0.239651 0.8029756 0.5666795 SLC2A3 solute carrier family 2 (facilitated glucose transporter), member 3 1026 +/- 38 880 +/- 62 1104 +/- 55 1085 +/- 58 0.021003 -0.078467 -0.818739 0.0242551 0.5835713 SLC35F2 solute carrier family 35, member F2 207 +/- 46 144 +/- 16 326 +/- 25 305 +/- 24 0.000324 -0.244329 -0.561463 0.3249204 0.5505928 SLC37A3 solute carrier family 37 (glycerol-3-phosphate transporter), member 3 931 +/- 71 1070 +/- 51 1261 +/- 82 1354 +/- 60 0.001297 0.1455695 0.387178 0.2447314 0.4459497 SLC39A13 solute carrier family 39 (zinc transporter), member 13 442 +/- 18 474 +/- 23 630 +/- 58 656 +/- 34 0.000119 0.3063952 0.707409 0.0912653 0.6165761 SLC44A2 solute carrier family 44, member 2 1048 +/- 76 1120 +/- 49 1283 +/- 77 1445 +/- 118 0.006709 0.4475349 0.283952 0.3156359 0.8745153 SLC4A10 solute carrier family 4, sodium bicarbonate transporter-like, member 10 1184 +/- 170 1069 +/- 138 1402 +/- 112 1397 +/- 63 0.029531 -0.610961 -0.968619 0.3256783 0.5149316 SLC4A4 solute carrier family 4, sodium bicarbonate cotransporter, member 4 157 +/- 10 174 +/- 20 254 +/- 30 241 +/- 14 0.00147 0.4829416 -0.713869 0.9127288 0.6282629 SLC5A3 solute carrier family 5 (inositol transporters), member 3 289 +/- 39 240 +/- 38 429 +/- 33 342 +/- 17 0.000569 -0.391169 -0.052976 0.3881746 0.0789157 SLC6A17 Solute carrier family 6, member 17 210 +/- 17 169 +/- 34 271 +/- 19 276 +/- 44 0.003758 -0.311296 0.914598 0.322681 0.7293514 SLC6A8 solute carrier family 6 (neurotransmitter transporter, creatine), member 8 1035 +/- 135 1096 +/- 125 1491 +/- 82 1629 +/- 86 0.00203 0.7458516 0.274069 0.6006384 0.5520465 SLC7A10 solute carrier family 7, (neutral amino acid transporter, y+ system) member 10 357 +/- 22 330 +/- 22 416 +/- 20 346 +/- 25 0.038904 -0.393374 -0.060576 0.3787784 0.0353852 SLC7A3 solute carrier family 7 (cationic amino acid transporter, y+ system), member 3 224 +/- 20 260 +/- 16 268 +/- 16 274 +/- 24 0.025146 0.2035323 0.859225 0.2463997 0.9711642 SLC7A8 solute carrier family 7 (cationic amino acid transporter, y+ system), member 8 154 +/- 24 138 +/- 24 223 +/- 35 238 +/- 30 0.031272 -0.661655 0.75489 0.7313684 0.9456271 SLC8A2 solute carrier family 8 (sodium-calcium exchanger), member 2 8880 +/- 579 7417 +/- 677 10805 +/- 749 9813 +/- 387 0.00037 -0.132175 -0.276414 0.0615612 0.2075135 SLC9A5 Solute carrier family 9 (sodium/hydrogen exchanger), member 5 79 +/- 8 66 +/- 16 153 +/- 21 117 +/- 16 0.001044 -0.490409 -0.218032 0.4030349 0.5140448 SLIT3 slit homolog 3 (Drosophila) 168 +/- 14 215 +/- 28 281 +/- 24 327 +/- 30 0.001512 0.1668762 0.264163 0.1731033 0.2279621 SLITRK3 SLIT and NTRK-like family, member 3 805 +/- 73 681 +/- 48 900 +/- 60 883 +/- 35 0.003988 -0.187426 -0.810348 0.1503791 0.5879921 SLU7 step II splicing factor SLU7 686 +/- 49 651 +/- 34 820 +/- 45 760 +/- 36 9.72E-06 -0.574939 -0.323368 0.9977697 0.4958945 SMAD1 SMAD, mothers against DPP homolog 1 (Drosophila) 643 +/- 44 720 +/- 16 780 +/- 22 738 +/- 27 0.015328 0.1515251 -0.25302 0.0856621 0.693038 SMAD3 SMAD, mothers against DPP homolog 3 (Drosophila) 188 +/- 33 121 +/- 27 344 +/- 37 309 +/- 37 0.000421 -0.148624 -0.522044 0.0728908 0.5333286 SMAD7 SMAD, mothers against DPP homolog 7 (Drosophila) 307 +/- 16 324 +/- 24 412 +/- 21 416 +/- 28 0.000386 0.5585009 0.910322 0.7755308 0.9310852 SMAD9 SMAD, mothers against DPP homolog 9 (Drosophila) 257 +/- 56 213 +/- 34 442 +/- 75 408 +/- 55 6.24E-05 -0.512901 -0.723789 0.5260257 0.819869 SMARCA1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, 952 +/- 143 852 +/- 93 1284 +/- 94 1301 +/- 131 0.001373 -0.572468 0.915481 0.495872 0.6066946 SMARCB1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, 1109 +/- 26 1171 +/- 34 1237 +/- 33 1243 +/- 59 0.040304 0.177362 0.93229 0.4453046 0.4318511 SMC1L1 SMC1 structural maintenance of chromosomes 1-like 1 (yeast) 109 +/- 11 124 +/- 5 124 +/- 8 155 +/- 12 0.002664 0.2579351 0.068434 0.1604097 0.012924 SMC5L1 SMC5 structural maintenance of chromosomes 5-like 1 (yeast) 80 +/- 7 105 +/- 11 119 +/- 18 132 +/- 16 0.0119 0.0903609 0.598996 0.0340661 0.9771966 SMCX Smcy homolog, X-linked (mouse) 532 +/- 25 498 +/- 36 799 +/- 43 712 +/- 66 0.00098 -0.46199 -0.306702 0.3722781 0.3141839 SMPD3 sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) 1229 +/- 172 973 +/- 155 1527 +/- 73 1365 +/- 83 0.004174 -0.294544 -0.178859 0.320479 0.3281313 SMS spermine synthase 1440 +/- 104 1361 +/- 60 1756 +/- 49 1533 +/- 42 0.001876 -0.531937 -0.00736 0.6064133 0.1179732

S2-genes differentially expressed across region.xls Page 28 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI SMYD2 SET and MYND domain containing 2 109 +/- 17 181 +/- 16 144 +/- 19 192 +/- 26 0.015144 0.0113553 0.180434 0.003035 0.0154565 SNAP25 Synaptosomal-associated protein, 25kDa 583 +/- 70 304 +/- 41 826 +/- 34 518 +/- 59 0.001182 -0.008466 -0.00321 0.0112881 0.0123304 SNAP91 synaptosomal-associated protein, 91kDa homolog (mouse) 10224 +/- 579 10352 +/- 809 10870 +/- 483 12419 +/- 359 0.016655 0.9003596 0.030731 0.7005781 0.2501644 SNAPC2 small nuclear RNA activating complex, polypeptide 2, 45kDa 561 +/- 35 472 +/- 29 611 +/- 43 565 +/- 27 0.009263 -0.081153 -0.396468 0.0297021 0.2882394 SNAPC3 small nuclear RNA activating complex, polypeptide 3, 50kDa 237 +/- 12 210 +/- 12 240 +/- 8 264 +/- 11 0.032721 -0.150438 0.122994 0.2219438 0.1821385 SNCA synuclein, alpha (non A4 component of amyloid precursor) 8572 +/- 460 8035 +/- 541 10395 +/- 780 10982 +/- 711 0.000525 -0.466771 0.591098 0.1529883 0.7778674 SNIP SNAP25-interacting protein 171 +/- 8 150 +/- 14 197 +/- 10 199 +/- 9 0.002983 -0.217767 0.8845 0.4745023 0.5472284 SNPH syntaphilin 2613 +/- 145 2551 +/- 229 3210 +/- 152 2979 +/- 147 0.000413 -0.824861 -0.304685 0.5676558 0.557265 SNRPD1 small nuclear ribonucleoprotein D1 polypeptide 16kDa 576 +/- 52 651 +/- 23 669 +/- 57 782 +/- 25 1.44E-05 0.2287422 0.116701 0.4277502 0.2528515 SNRPN small nuclear ribonucleoprotein polypeptide N 132 +/- 14 163 +/- 31 176 +/- 10 237 +/- 52 0.017771 0.3858409 0.318396 0.1257248 0.0182757 SNX4 sorting nexin 4 300 +/- 31 376 +/- 26 403 +/- 33 453 +/- 12 0.001799 0.0956579 0.213336 0.0266034 0.2046534 SNX9 sorting nexin 9 1081 +/- 34 1199 +/- 60 1155 +/- 37 1282 +/- 40 0.012901 0.1251068 0.045268 0.0200866 0.0494946 SOCS1 suppressor of cytokine signaling 1 193 +/- 12 114 +/- 15 214 +/- 14 159 +/- 18 0.001196 -0.002148 -0.038543 0.0006493 0.0014373 SORBS1 sorbin and SH3 domain containing 1 688 +/- 45 536 +/- 41 818 +/- 42 620 +/- 39 0.045933 -0.032044 -0.007103 0.0445536 0.0045809 SORBS3 sorbin and SH3 domain containing 3 492 +/- 36 495 +/- 36 591 +/- 39 549 +/- 15 0.048526 0.9556806 -0.352191 0.7225567 0.3175566 SORD sorbitol dehydrogenase 145 +/- 14 169 +/- 20 200 +/- 17 225 +/- 15 0.001378 0.3531917 0.297006 0.9720434 0.7361801 SOX1 SRY (sex determining region Y)-box 1 375 +/- 39 272 +/- 18 455 +/- 28 336 +/- 23 0.006275 -0.050161 -0.010004 0.1370089 0.0085711 SOX12 SRY (sex determining region Y)-box 12 320 +/- 19 281 +/- 25 370 +/- 17 338 +/- 14 0.02039 -0.24604 -0.183594 0.2677755 0.3727289 SOX21 SRY (sex determining region Y)-box 21 152 +/- 17 149 +/- 21 246 +/- 19 235 +/- 20 0.003097 -0.91847 -0.703642 0.9548535 0.4735048 SOX9 SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal) 2092 +/- 250 2332 +/- 152 3201 +/- 297 3377 +/- 147 5.26E-05 0.4345332 0.611422 0.7155578 0.8029809 SP3 Sp3 transcription factor 192 +/- 6 217 +/- 16 257 +/- 16 258 +/- 16 0.004414 0.1733608 0.9805 0.0307224 0.9935729 SPAG5 sperm associated antigen 5 430 +/- 73 461 +/- 68 641 +/- 77 620 +/- 57 0.030989 0.7603672 -0.829771 0.9383403 0.6253147 SPATA5L1 spermatogenesis associated 5-like 1 165 +/- 23 207 +/- 16 215 +/- 12 299 +/- 49 0.012911 0.1620911 0.161599 0.2291866 0.4044434 SPATS2 spermatogenesis associated, serine-rich 2 189 +/- 17 228 +/- 22 259 +/- 31 311 +/- 24 0.003773 0.1994109 0.211877 0.1310841 0.2909312 SPEG SPEG complex locus 382 +/- 16 419 +/- 56 489 +/- 33 545 +/- 69 0.001074 0.550069 0.485418 0.1441813 0.2410492 SPIN3 spindlin family, member 3 583 +/- 41 531 +/- 70 834 +/- 37 749 +/- 30 0.00046 -0.544367 -0.112436 0.4072844 0.0904056 SPINLW1 serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) 118 +/- 6 137 +/- 15 187 +/- 9 158 +/- 15 0.011178 0.2607438 -0.147915 0.2346082 0.0700427 SPOCD1 SPOC domain containing 1 158 +/- 17 118 +/- 16 211 +/- 18 162 +/- 27 0.008586 -0.123117 -0.169082 0.224603 0.5423912 SPOCK1 sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1 7167 +/- 214 7938 +/- 1021 13027 +/- 650 13078 +/- 476 2.33E-06 0.4916382 0.950583 0.84795 0.879922 SPPL2B signal peptide peptidase-like 2B 326 +/- 22 313 +/- 24 418 +/- 18 351 +/- 27 0.00919 -0.707456 -0.0787 0.9510915 0.3276354 SPRN shadow of prion protein homolog (zebrafish) 269 +/- 38 230 +/- 17 364 +/- 21 375 +/- 25 0.000965 -0.378278 0.741331 0.0754887 0.5748826 SPRYD3 SPRY domain containing 3 4819 +/- 160 4681 +/- 224 5328 +/- 112 5128 +/- 120 0.01062 -0.630231 -0.254198 0.1993207 0.1067404 SPTAN1 spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) 7252 +/- 431 6761 +/- 337 7762 +/- 270 7878 +/- 221 0.014299 -0.392515 0.746458 0.1154177 0.862094 SPTB spectrin, beta, erythrocytic (includes spherocytosis, clinical type I) 269 +/- 8 261 +/- 20 349 +/- 22 330 +/- 23 0.002036 -0.711673 -0.571083 0.9517376 0.8940819 SPTBN1 spectrin, beta, non-erythrocytic 1 1270 +/- 153 835 +/- 88 1365 +/- 200 1056 +/- 161 0.020923 -0.038683 -0.260652 0.1486036 0.4901887 SPTBN2 spectrin, beta, non-erythrocytic 2 1861 +/- 231 1513 +/- 170 2833 +/- 203 2733 +/- 201 0.000275 -0.25685 -0.733922 0.1506683 0.8724221 SR-A1 serine arginine-rich pre-mRNA splicing factor SR-A1 440 +/- 30 527 +/- 58 660 +/- 33 789 +/- 43 3.71E-05 0.2204766 0.046113 0.8153769 0.1057452 SREBF2 sterol regulatory element binding transcription factor 2 1173 +/- 80 1157 +/- 64 1650 +/- 13 1844 +/- 70 1.99E-05 -0.882991 0.049562 0.5901255 0.2589213 SRM spermidine synthase 1721 +/- 23 1637 +/- 95 1864 +/- 78 1890 +/- 56 0.009582 -0.423893 0.79391 0.1461832 0.9354647 SRP9 signal recognition particle 9kDa 4130 +/- 251 4473 +/- 110 4814 +/- 303 4892 +/- 242 0.006342 0.2511049 0.845472 0.2851241 0.9372765 SRXN1 sulfiredoxin 1 homolog (S. cerevisiae) 1120 +/- 71 1170 +/- 51 1218 +/- 61 1286 +/- 56 0.030218 0.5755012 0.437612 0.9049488 0.6239331 SSBP4 single stranded DNA binding protein 4 1116 +/- 95 896 +/- 82 1436 +/- 99 1297 +/- 62 0.000873 -0.111448 -0.266965 0.0710235 0.2834373 SSR4 Signal sequence receptor, delta (translocon-associated protein delta) 211 +/- 36 243 +/- 27 273 +/- 15 296 +/- 28 0.002643 0.4919386 0.496365 0.3513944 0.1385836 SSRP1 structure specific recognition protein 1 461 +/- 41 489 +/- 26 598 +/- 31 625 +/- 24 0.001692 0.575885 0.497805 0.7468257 0.3328728 ST8SIA4 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 420 +/- 36 487 +/- 18 530 +/- 45 521 +/- 14 0.030783 0.1405574 -0.854865 0.2535427 0.8085928

S2-genes differentially expressed across region.xls Page 29 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI STAU1 Staufen, RNA binding protein, homolog 1 (Drosophila) 135 +/- 17 151 +/- 17 155 +/- 8 159 +/- 7 0.023236 0.5348711 0.685589 0.2607829 0.6094558 STC1 stanniocalcin 1 166 +/- 23 116 +/- 17 235 +/- 30 186 +/- 29 0.004022 -0.112545 -0.272351 0.1284177 0.4235365 STEAP3 STEAP family member 3 95 +/- 19 83 +/- 5 159 +/- 18 149 +/- 19 0.000498 -0.55905 -0.720709 0.8429664 0.4117541 STH saitohin 204 +/- 19 176 +/- 18 224 +/- 25 274 +/- 26 0.011096 -0.30825 0.194955 0.2280277 0.2497963 STK11 serine/threonine kinase 11 840 +/- 24 788 +/- 36 928 +/- 20 849 +/- 36 0.011475 -0.270219 -0.101026 0.0078114 0.0298969 STK32B serine/threonine kinase 32B 243 +/- 24 228 +/- 32 384 +/- 31 326 +/- 32 0.002198 -0.711977 -0.220768 0.4002021 0.0260982 STK38 serine/threonine kinase 38 341 +/- 28 391 +/- 25 433 +/- 21 437 +/- 26 0.003453 0.2154051 0.888895 0.3961512 0.8886195 STRN4 striatin, calmodulin binding protein 4 2259 +/- 118 2127 +/- 112 3229 +/- 188 3070 +/- 160 5.2E-05 -0.436998 -0.536684 0.4629875 0.8324429 STX17 syntaxin 17 148 +/- 13 149 +/- 12 205 +/- 19 180 +/- 21 0.02549 0.9697172 -0.41372 0.6681478 0.2259742 STX1B2 Syntaxin 1B2 2479 +/- 136 2482 +/- 193 2925 +/- 136 3039 +/- 90 0.005405 0.9900455 0.506406 0.3052837 0.4007239 SUB1 SUB1 homolog (S. cerevisiae) 198 +/- 12 153 +/- 13 233 +/- 7 187 +/- 11 0.008317 -0.029381 -0.009334 0.0705728 0.2008498 SUGT1 SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) 266 +/- 18 181 +/- 12 342 +/- 23 283 +/- 32 0.000158 -0.003216 -0.181555 0.0325977 0.7391313 SUHW3 suppressor of hairy wing homolog 3 (Drosophila) 164 +/- 8 195 +/- 7 232 +/- 8 239 +/- 11 0.000226 0.0123894 0.663982 0.0018506 0.4146132 SUMO1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) 319 +/- 19 270 +/- 20 387 +/- 34 307 +/- 18 0.033679 -0.100938 -0.073092 0.3731749 0.2069742 SUMO2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) 12943 +/- 496 11884 +/- 615 16502 +/- 609 16284 +/- 549 2.99E-05 -0.211266 -0.796145 0.0992828 0.7056612 SUPT6H suppressor of Ty 6 homolog (S. cerevisiae) 1172 +/- 55 1281 +/- 91 1334 +/- 101 1469 +/- 81 0.002814 0.3359974 0.328502 0.8999097 0.9211334 SURF6 surfeit 6 376 +/- 23 356 +/- 13 414 +/- 22 397 +/- 25 0.001643 -0.46303 -0.637214 0.367044 0.6966128 SUSD1 sushi domain containing 1 189 +/- 19 169 +/- 14 260 +/- 28 234 +/- 13 0.001415 -0.415794 -0.424985 0.1215955 0.5501742 SUSD4 sushi domain containing 4 4749 +/- 449 3837 +/- 255 5238 +/- 182 5024 +/- 172 0.004361 -0.115599 -0.414886 0.1190265 0.4527459 SUV420H1Suppressor of variegation 4-20 homolog 1 (Drosophila) 123 +/- 7 134 +/- 6 175 +/- 16 167 +/- 11 0.000595 0.2879019 -0.682286 0.1197948 0.9587164 SWAP70 SWAP-70 protein 261 +/- 38 328 +/- 44 534 +/- 48 549 +/- 36 0.000669 0.2799525 0.813608 0.6487726 0.7354966 SYN1 synapsin I 9377 +/- 166 8662 +/- 478 9876 +/- 310 9961 +/- 440 0.013961 -0.206004 0.878363 0.0050647 0.8227875 SYNJ2BP Synaptojanin 2 binding protein 752 +/- 43 787 +/- 33 1031 +/- 19 944 +/- 14 6.14E-05 0.5393535 -0.005859 0.1846546 0.0128158 SYNPR synaptoporin 7801 +/- 1027 6762 +/- 1198 12700 +/- 298 11939 +/- 542 5.49E-05 -0.52563 -0.262481 0.6085074 0.718369 SYT13 synaptotagmin XIII 3220 +/- 472 4234 +/- 268 3776 +/- 271 4951 +/- 494 0.029122 0.0988615 0.079487 0.2194171 0.1377098 SYT4 synaptotagmin IV 9719 +/- 546 8818 +/- 490 10923 +/- 459 10364 +/- 486 0.00467 -0.247397 -0.424941 0.3130202 0.8516169 SYT5 synaptotagmin V 1590 +/- 119 1315 +/- 96 2095 +/- 145 1945 +/- 55 3.29E-05 -0.1048 -0.367943 0.0417789 0.3913412 SYT7 synaptotagmin VII 114 +/- 17 89 +/- 14 257 +/- 20 201 +/- 21 2.81E-05 -0.287383 -0.086561 0.5795385 0.2106884 TACR1 tachykinin receptor 1 118 +/- 3 103 +/- 12 193 +/- 23 142 +/- 11 0.002369 -0.259958 -0.085604 0.1369636 0.091764 TACSTD1 tumor-associated calcium signal transducer 1 86 +/- 16 85 +/- 5 123 +/- 14 124 +/- 16 0.003462 -0.925072 0.965966 0.8481644 0.7204601 TAF1B TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa 234 +/- 23 246 +/- 38 330 +/- 22 422 +/- 23 0.000316 0.7891915 0.019503 0.8921318 0.0425003 TAF1L TAF1-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 210kDa 212 +/- 20 232 +/- 11 284 +/- 14 279 +/- 6 0.001271 0.4072719 -0.736238 0.2955867 0.9889043 TANC1 tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1 1471 +/- 317 1200 +/- 295 2982 +/- 167 2612 +/- 197 1.88E-05 -0.545908 -0.187932 0.7487271 0.3194197 TANC2 tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2 183 +/- 32 149 +/- 22 226 +/- 11 226 +/- 9 0.024707 -0.408692 -0.998032 0.4164271 0.9535105 TAOK1 TAO kinase 1 173 +/- 17 198 +/- 24 218 +/- 20 226 +/- 29 0.033917 0.4185466 0.823241 0.0750809 0.4564026 TAOK2 TAO kinase 2 625 +/- 34 519 +/- 39 790 +/- 45 709 +/- 38 0.000751 -0.068182 -0.196187 0.0138477 0.2934772 TARSL1 threonyl-tRNA synthetase-like 1 436 +/- 27 420 +/- 16 475 +/- 27 483 +/- 12 0.010493 -0.628132 0.796268 0.6217059 0.9312237 TASP1 taspase, threonine aspartase, 1 270 +/- 19 225 +/- 18 323 +/- 12 254 +/- 14 0.006132 -0.113118 -0.005654 0.0680737 0.0494189 TBC1D19 TBC1 domain family, member 19 395 +/- 25 343 +/- 13 415 +/- 31 417 +/- 24 0.043573 -0.099158 0.968567 0.0678114 0.9021212 TBC1D24 TBC1 domain family, member 24 2474 +/- 168 2207 +/- 117 2868 +/- 190 2598 +/- 160 0.001489 -0.22621 -0.305466 0.0484201 0.1543133 TBCCD1 TBCC domain containing 1 233 +/- 24 296 +/- 18 329 +/- 21 322 +/- 5 0.009985 0.0619208 -0.733815 0.2555343 0.6875354 TBL1X transducin (beta)-like 1X-linked 155 +/- 28 369 +/- 21 212 +/- 17 525 +/- 99 0.040271 0.0001457 0.033161 0.0033462 0.0739863 tcag7.981 juxtaposed with another zinc finger gene 1 381 +/- 39 292 +/- 28 458 +/- 39 490 +/- 40 0.007887 -0.096156 0.578672 0.0446762 0.7339668 TCEAL8 transcription elongation factor A (SII)-like 8 2261 +/- 121 2724 +/- 87 2754 +/- 147 2815 +/- 38 0.006499 0.012366 0.698956 0.0146709 0.6065382 TCF4 transcription factor 4 4852 +/- 158 4083 +/- 243 6014 +/- 463 4769 +/- 717 0.034252 -0.027614 -0.187716 0.3837346 0.9305804

S2-genes differentially expressed across region.xls Page 30 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI TEAD1 TEA domain family member 1 (SV40 transcriptional enhancer factor) 138 +/- 24 120 +/- 19 207 +/- 11 183 +/- 24 0.003691 -0.552191 -0.404854 0.7806656 0.5873142 TFAP2C transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) 162 +/- 7 171 +/- 15 220 +/- 12 192 +/- 14 0.006882 0.6086945 -0.160198 0.7618906 0.0750851 TFE3 transcription factor binding to IGHM enhancer 3 464 +/- 38 530 +/- 20 669 +/- 32 746 +/- 49 0.000306 0.1647344 0.2308 0.7114555 0.591942 TGFB2 Transforming growth factor, beta 2 228 +/- 32 306 +/- 56 395 +/- 33 454 +/- 28 0.005897 0.2570186 0.213191 0.4591064 0.1115706 TGFB3 transforming growth factor, beta 3 505 +/- 61 393 +/- 48 651 +/- 37 486 +/- 46 0.001231 -0.182678 -0.022851 0.548311 0.08447 THAP11 THAP domain containing 11 1105 +/- 29 934 +/- 18 1207 +/- 52 1091 +/- 55 0.014562 -0.000838 -0.162464 0.0183226 0.0649289 THBS3 thrombospondin 3 546 +/- 53 445 +/- 55 780 +/- 44 597 +/- 55 0.002165 -0.214844 -0.03159 0.2819204 0.0687474 THNSL1 threonine synthase-like 1 (bacterial) 904 +/- 69 911 +/- 73 1077 +/- 20 1202 +/- 28 0.002795 0.9472046 0.007608 0.7074732 0.0448758 THOC3 THO complex 3 822 +/- 43 768 +/- 27 912 +/- 33 917 +/- 44 0.006396 -0.314602 0.926553 0.7553929 0.4127478 THRAP1 thyroid hormone receptor associated protein 1 2516 +/- 97 2385 +/- 106 2836 +/- 108 2482 +/- 58 0.047824 -0.383922 -0.021616 0.9182598 0.0677997 THUMPD1 THUMP domain containing 1 742 +/- 69 810 +/- 24 942 +/- 25 840 +/- 57 0.04308 0.389768 -0.156107 0.1551043 0.7150661 TIA1 TIA1 cytotoxic granule-associated RNA binding protein 874 +/- 68 1007 +/- 33 956 +/- 62 1060 +/- 49 0.0472 0.1217875 0.221386 0.0524667 0.4665781 TIAL1 TIA1 cytotoxic granule-associated RNA binding protein-like 1 649 +/- 26 712 +/- 30 716 +/- 24 763 +/- 39 0.040576 0.1379057 0.340948 0.2041902 0.9254245 TIAM1 T-cell lymphoma invasion and metastasis 1 7165 +/- 523 6210 +/- 579 8375 +/- 702 7935 +/- 526 0.039446 -0.249449 -0.628303 0.2288296 0.735679 TIFA TRAF-interacting protein with a forkhead-associated domain 97 +/- 8 121 +/- 14 110 +/- 14 155 +/- 22 0.049453 0.1748475 0.128136 0.2620997 0.3263587 TIGD1 tigger transposable element derived 1 131 +/- 10 163 +/- 16 178 +/- 9 158 +/- 6 0.03645 0.1265411 -0.099891 0.0570281 0.3808026 TIGD4 Tigger transposable element derived 4 169 +/- 19 172 +/- 8 219 +/- 22 201 +/- 18 0.037805 0.8777507 -0.533143 0.5864319 0.7218253 TIMM9 translocase of inner mitochondrial membrane 9 homolog (yeast) 1054 +/- 79 1023 +/- 58 1314 +/- 116 1222 +/- 67 0.029722 -0.758442 -0.516216 0.6882885 0.8239718 TLCD1 TLC domain containing 1 244 +/- 18 250 +/- 25 290 +/- 13 305 +/- 19 0.00131 0.8458537 0.533168 0.4851929 0.534917 TM2D3 TM2 domain containing 3 1598 +/- 99 1706 +/- 91 1817 +/- 112 1766 +/- 67 0.003348 0.4378893 -0.706016 0.6280413 0.9190701 TMCC1 Transmembrane and coiled-coil domain family 1 100 +/- 14 167 +/- 12 175 +/- 18 218 +/- 27 0.008644 0.0045566 0.232236 0.002973 0.630778 TMEM101 transmembrane protein 101 675 +/- 42 618 +/- 45 750 +/- 17 705 +/- 15 0.01854 -0.369877 -0.080463 0.3060146 0.1110347 TMEM106ATransmembrane protein 106A 262 +/- 38 357 +/- 34 391 +/- 26 405 +/- 32 0.03326 0.0927646 0.754754 0.0391729 0.482147 TMEM106Btransmembrane protein 106B 4831 +/- 291 4025 +/- 238 5533 +/- 280 4811 +/- 200 0.007133 -0.058398 -0.066771 0.150374 0.086534 TMEM132Etransmembrane protein 132E 138 +/- 10 118 +/- 18 232 +/- 19 159 +/- 19 0.003245 -0.347428 -0.023539 0.1210173 0.0709417 TMEM145 transmembrane protein 145 217 +/- 45 302 +/- 45 461 +/- 25 437 +/- 46 0.004792 0.2133024 -0.656727 0.2795145 0.5993333 TMEM155 transmembrane protein 155 681 +/- 159 731 +/- 102 1005 +/- 95 952 +/- 109 0.005603 0.7964121 -0.719275 0.8814584 0.5369674 TMEM157 transmembrane protein 157 1071 +/- 81 1357 +/- 141 1396 +/- 135 1514 +/- 51 0.00413 0.1169164 0.443375 0.3751543 0.860073 TMEM17 transmembrane protein 17 1201 +/- 190 943 +/- 121 1496 +/- 81 1397 +/- 26 0.002791 -0.284126 -0.288136 0.2533249 0.2733874 TMEM2 transmembrane protein 2 579 +/- 105 540 +/- 122 879 +/- 63 937 +/- 51 0.001128 -0.816285 0.492065 0.8577714 0.1842878 TMEM25 transmembrane protein 25 2517 +/- 184 2187 +/- 216 3846 +/- 199 3254 +/- 106 0.000106 -0.27173 -0.032156 0.2729187 0.1297675 TMEM34 transmembrane protein 34 461 +/- 47 399 +/- 28 561 +/- 32 509 +/- 34 0.013101 -0.288718 -0.288026 0.213151 0.1128186 TMEM35 transmembrane protein 35 3372 +/- 336 2655 +/- 290 3993 +/- 156 3988 +/- 198 0.000691 -0.138043 -0.982368 0.0911204 0.490275 TMEM50A transmembrane protein 50A 3252 +/- 47 2957 +/- 223 4076 +/- 139 3448 +/- 34 0.001072 -0.248078 -0.005475 0.4666802 0.025314 TMEM57 transmembrane protein 57 604 +/- 27 705 +/- 15 723 +/- 33 725 +/- 8 0.046032 0.0126412 0.945545 0.0242234 0.8980879 TMEM69 transmembrane protein 69 384 +/- 5 399 +/- 22 508 +/- 26 437 +/- 37 0.032387 0.5290993 -0.159565 0.6220057 0.009146 TMEM76 transmembrane protein 76 3761 +/- 96 3217 +/- 173 4452 +/- 182 4256 +/- 291 0.000104 -0.025905 -0.587721 0.0215696 0.3439686 TNC Tenascin C (hexabrachion) 123 +/- 12 95 +/- 12 170 +/- 14 135 +/- 16 0.005698 -0.134881 -0.136861 0.1756168 0.0793545 TNFAIP3 tumor necrosis factor, alpha-induced protein 3 384 +/- 33 430 +/- 22 498 +/- 36 553 +/- 20 3.42E-06 0.2742655 0.217638 0.6023076 0.7698438 TNFAIP8L1tumor necrosis factor, alpha-induced protein 8-like 1 625 +/- 37 647 +/- 29 658 +/- 40 775 +/- 14 0.03887 0.6471604 0.032229 0.8891392 0.0755139 TNFRSF19tumor necrosis factor receptor superfamily, member 19 113 +/- 17 70 +/- 3 136 +/- 13 101 +/- 8 0.022313 -0.052431 -0.056666 0.0367617 0.1181179 TNFRSF21tumor necrosis factor receptor superfamily, member 21 1253 +/- 128 1127 +/- 85 1726 +/- 160 1813 +/- 182 0.000373 -0.435265 0.73039 0.2155143 0.9284655 TNFRSF25tumor necrosis factor receptor superfamily, member 25 329 +/- 55 221 +/- 33 476 +/- 65 375 +/- 17 0.001449 -0.127113 -0.18511 0.1017872 0.2260367 TNIK TRAF2 and NCK interacting kinase 1817 +/- 199 1891 +/- 141 2203 +/- 142 2032 +/- 65 0.042747 0.7697162 -0.309801 0.8328915 0.4567642 TNPO1 Transportin 1 463 +/- 30 493 +/- 19 563 +/- 32 586 +/- 23 0.002662 0.4321005 0.574342 0.2614933 0.5518329

S2-genes differentially expressed across region.xls Page 31 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI TNRC4 trinucleotide repeat containing 4 4534 +/- 210 3677 +/- 260 5558 +/- 185 4760 +/- 252 0.000551 -0.029148 -0.035167 0.0107826 0.1256802 TNRC6B trinucleotide repeat containing 6B 1117 +/- 123 1256 +/- 41 1325 +/- 75 1510 +/- 107 0.000842 0.3218746 0.199193 0.4025022 0.1235851 TNRC6C trinucleotide repeat containing 6C 152 +/- 17 203 +/- 20 220 +/- 12 223 +/- 22 0.039677 0.0849266 0.933611 0.020591 0.837777 TOB1 transducer of ERBB2, 1 172 +/- 34 132 +/- 24 260 +/- 18 168 +/- 38 0.034751 -0.356433 -0.07453 0.2580621 0.0374096 TOM1L1 Target of myb1-like 1 (chicken) 114 +/- 13 121 +/- 6 130 +/- 12 171 +/- 11 0.025777 0.6318576 0.030309 0.6264392 0.0957465 TOM1L2 target of myb1-like 2 (chicken) 427 +/- 53 454 +/- 32 627 +/- 29 729 +/- 42 0.000143 0.6758655 0.083306 0.7544948 0.0737582 TOMM20 translocase of outer mitochondrial membrane 20 homolog (yeast) 2077 +/- 219 1780 +/- 210 2535 +/- 203 2410 +/- 320 0.00979 -0.350615 -0.74992 0.1375626 0.5406237 TOPORS topoisomerase I binding, arginine/serine-rich 675 +/- 31 711 +/- 19 808 +/- 28 789 +/- 20 0.00139 0.3338017 -0.58379 0.0562058 0.8958109 TOR1AIP1 torsin A interacting protein 1 848 +/- 70 1089 +/- 39 1211 +/- 42 1195 +/- 20 0.002503 0.0176232 -0.739001 0.0164243 0.7918809 TP53BP1 tumor protein p53 binding protein, 1 161 +/- 33 128 +/- 20 228 +/- 14 192 +/- 13 0.00526 -0.425918 -0.094506 0.3988633 0.4583462 TP53I11 tumor protein p53 inducible protein 11 260 +/- 27 226 +/- 20 441 +/- 27 402 +/- 21 2.92E-05 -0.3405 -0.289464 0.1040261 0.6878425 TPARL TPA regulated locus 2090 +/- 91 1940 +/- 66 2330 +/- 87 2232 +/- 49 0.014825 -0.218201 -0.352865 0.3419041 0.4953587 TPD52 tumor protein D52 200 +/- 13 258 +/- 21 346 +/- 17 380 +/- 12 1.36E-06 0.0473867 0.132678 0.1577976 0.2435336 TPM1 Tropomyosin 1 (alpha) 396 +/- 71 679 +/- 70 626 +/- 100 856 +/- 98 0.000756 0.0180423 0.135836 0.0174101 0.0760796 TPM3 Tropomyosin 3 66 +/- 18 93 +/- 16 149 +/- 12 140 +/- 8 0.001461 0.2891188 -0.537357 0.2336129 0.2107406 TPP2 tripeptidyl peptidase II 551 +/- 39 474 +/- 52 619 +/- 20 581 +/- 36 0.026383 -0.261144 -0.386592 0.152663 0.2232007 TPT1 tumor protein, translationally-controlled 1 440 +/- 33 330 +/- 27 527 +/- 42 469 +/- 48 0.001767 -0.028535 -0.385139 0.0312002 0.4178308 TRA2A transformer-2 alpha 1956 +/- 84 1639 +/- 102 2458 +/- 104 1919 +/- 80 0.000253 -0.03851 -0.002777 0.3163716 0.022919 TRAF3 TNF receptor-associated factor 3 1143 +/- 89 1002 +/- 66 1473 +/- 46 1224 +/- 60 0.001402 -0.233136 -0.011177 0.193721 0.0476289 TRAK1 trafficking protein, kinesin binding 1 827 +/- 63 630 +/- 58 1039 +/- 46 885 +/- 23 0.000187 -0.044996 -0.018605 0.0166329 0.0124228 TRAPPC1 trafficking protein particle complex 1 2834 +/- 124 2513 +/- 112 3260 +/- 100 3381 +/- 157 0.000489 -0.084079 0.533986 0.062045 0.4098492 TREX2 three prime repair exonuclease 2 95 +/- 10 117 +/- 10 117 +/- 14 160 +/- 11 0.001483 0.1529645 0.039352 0.6087976 0.2772861 TRFP Trf (TATA binding protein-related factor)-proximal homolog (Drosophila) 760 +/- 31 669 +/- 45 899 +/- 11 837 +/- 43 0.000485 -0.131777 -0.229599 0.3502448 0.9840086 TRIM25 tripartite motif-containing 25 564 +/- 66 671 +/- 95 691 +/- 50 799 +/- 33 0.023927 0.3766349 0.108873 0.5719798 0.2297354 TRIM28 tripartite motif-containing 28 2148 +/- 73 2098 +/- 79 2740 +/- 118 2779 +/- 71 0.000141 -0.652851 0.784704 0.4603828 0.6012828 TRIM33 tripartite motif-containing 33 174 +/- 16 183 +/- 20 252 +/- 21 241 +/- 25 0.009426 0.7354711 -0.743118 0.9353268 0.3024073 TRIO Triple functional domain (PTPRF interacting) 232 +/- 36 388 +/- 49 416 +/- 22 423 +/- 36 0.012866 0.0303455 0.867579 0.15146 0.6239491 TRIT1 tRNA isopentenyltransferase 1 289 +/- 26 281 +/- 55 428 +/- 45 417 +/- 54 0.003712 -0.90035 -0.890699 0.3481056 0.3372158 TSEN2 tRNA splicing endonuclease 2 homolog (S. cerevisiae) 680 +/- 36 775 +/- 24 764 +/- 50 848 +/- 36 0.036102 0.0587654 0.200491 0.200548 0.3558005 TSHZ1 teashirt family zinc finger 1 1426 +/- 157 1624 +/- 111 1877 +/- 150 2037 +/- 168 0.027596 0.3298796 0.497611 0.4602512 0.3670014 TSPAN17 tetraspanin 17 446 +/- 46 438 +/- 39 552 +/- 47 559 +/- 56 0.001608 -0.900023 0.924545 0.6270373 0.8927033 TSPAN33 tetraspanin 33 602 +/- 55 629 +/- 49 799 +/- 8 815 +/- 30 6.61E-05 0.7228646 0.624172 0.5972228 0.5699226 TSPYL3 TSPY-like 3 (pseudogene) 716 +/- 53 638 +/- 63 867 +/- 80 645 +/- 47 0.049766 -0.361301 -0.04429 0.4325361 0.1425855 TSPYL5 TSPY-like 5 2033 +/- 101 1907 +/- 210 2531 +/- 101 2467 +/- 60 0.000968 -0.603119 -0.598137 0.186607 0.1726726 TSR1 TSR1, 20S rRNA accumulation, homolog (S. cerevisiae) 478 +/- 37 400 +/- 29 506 +/- 20 512 +/- 34 0.035658 -0.129096 0.892873 0.1401649 0.9607942 TSTA3 tissue specific transplantation antigen P35B 575 +/- 27 585 +/- 20 622 +/- 26 649 +/- 29 0.024056 0.7729235 0.501225 0.8804125 0.9551662 TTC13 Tetratricopeptide repeat domain 13 138 +/- 8 126 +/- 8 200 +/- 12 191 +/- 19 3.46E-05 -0.322786 -0.717409 0.643149 0.9171223 TTC19 tetratricopeptide repeat domain 19 2924 +/- 101 1559 +/- 359 3189 +/- 138 1757 +/- 528 0.051201 -0.011318 -0.051447 0.0656807 0.1680265 TTC21A tetratricopeptide repeat domain 21A 332 +/- 47 407 +/- 38 457 +/- 68 502 +/- 47 0.016501 0.247463 0.596643 0.2961846 0.3642178 TTC3 tetratricopeptide repeat domain 3 2645 +/- 419 2041 +/- 301 3000 +/- 226 3160 +/- 310 0.046395 -0.271423 0.68729 0.2178277 0.9574265 TTC7B tetratricopeptide repeat domain 7B 3304 +/- 232 3005 +/- 181 3535 +/- 88 3570 +/- 154 0.045743 -0.334656 0.847084 0.2314993 0.1073848 TTC9B tetratricopeptide repeat domain 9B 1423 +/- 122 1065 +/- 141 1668 +/- 84 1424 +/- 82 0.038822 -0.084279 -0.068593 0.0566297 0.1299875 TTLL3 tubulin tyrosine ligase-like family, member 3 482 +/- 36 418 +/- 52 618 +/- 61 483 +/- 42 0.006441 -0.343168 -0.103728 0.0970144 0.0704493 TTYH3 tweety homolog 3 (Drosophila) 919 +/- 53 806 +/- 80 1280 +/- 86 1140 +/- 79 0.001661 -0.2702 -0.26088 0.0512111 0.1536357 TUSC1 tumor suppressor candidate 1 965 +/- 92 888 +/- 78 1306 +/- 67 1105 +/- 34 0.012341 -0.537009 -0.031015 0.4691942 0.0926691

S2-genes differentially expressed across region.xls Page 32 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI TUSC3 tumor suppressor candidate 3 593 +/- 43 437 +/- 30 743 +/- 35 545 +/- 36 0.00246 -0.015208 -0.003574 0.0268112 0.0789955 TWISTNB TWIST neighbor 120 +/- 10 136 +/- 17 170 +/- 19 172 +/- 10 0.003003 0.4217582 0.937788 0.4335335 0.7887629 TXNDC thioredoxin domain containing 476 +/- 65 553 +/- 69 609 +/- 50 751 +/- 51 0.014994 0.4355608 0.077209 0.2584736 0.2376259 TXNL1 thioredoxin-like 1 2413 +/- 328 2952 +/- 186 3020 +/- 129 3692 +/- 218 0.011617 0.1909979 0.03449 0.2960004 0.0595136 TYW3 tRNA-yW synthesizing protein 3 homolog (S. cerevisiae) 375 +/- 42 369 +/- 42 440 +/- 45 426 +/- 47 0.020135 -0.918334 -0.839601 0.5098858 0.5781038 UBE1 ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing) 6200 +/- 205 6514 +/- 169 6473 +/- 145 7267 +/- 148 0.00557 0.2658432 0.004142 0.8529343 0.0520709 UBE1C Ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) 132 +/- 17 140 +/- 15 180 +/- 15 161 +/- 7 0.042562 0.7276579 -0.268491 0.6819736 0.3028668 UBE1DC1 ubiquitin-activating enzyme E1-domain containing 1 944 +/- 81 433 +/- 60 1060 +/- 122 436 +/- 35 0.016502 -0.00065 -0.00298 0.0148963 0.0210426 UBE2E3 Ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast) 228 +/- 39 362 +/- 76 367 +/- 52 438 +/- 93 0.030393 0.1566628 0.529079 0.4646421 0.9420953 UBE2J1 ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) 447 +/- 25 412 +/- 15 587 +/- 24 472 +/- 9 0.001425 -0.256295 -0.003566 0.1363247 0.0170116 UBE2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) 1036 +/- 59 1047 +/- 67 1358 +/- 89 1401 +/- 79 0.001926 0.9009209 0.72824 0.8286661 0.994021 UBE2R2 ubiquitin-conjugating enzyme E2R 2 1064 +/- 43 984 +/- 33 1216 +/- 47 1128 +/- 17 0.006447 -0.169439 -0.126029 0.2372278 0.2827777 UBE3B ubiquitin protein ligase E3B 1301 +/- 22 1234 +/- 44 1463 +/- 39 1389 +/- 48 0.000393 -0.215786 -0.26552 0.2228435 0.4061134 UBLCP1 ubiquitin-like domain containing CTD phosphatase 1 704 +/- 65 599 +/- 48 1007 +/- 130 701 +/- 68 0.003007 -0.22025 -0.072872 0.1877406 0.0890775 UBPH ubiquitin-binding protein homolog 1695 +/- 104 1607 +/- 42 1871 +/- 43 1800 +/- 84 0.018389 -0.456352 -0.476111 0.7825261 0.7518558 UBR1 ubiquitin protein ligase E3 component n-recognin 1 655 +/- 48 599 +/- 38 837 +/- 25 692 +/- 31 0.002617 -0.387825 -0.006326 0.393552 0.0068199 UBTF upstream binding transcription factor, RNA polymerase I 637 +/- 57 695 +/- 48 792 +/- 49 824 +/- 23 0.006395 0.4502225 0.577258 0.4149284 0.9642524 UBXD1 UBX domain containing 1 2280 +/- 115 2689 +/- 177 2731 +/- 204 3215 +/- 112 0.000632 0.0862916 0.072838 0.4621686 0.131601 UEV3 ubiquitin-conjugating enzyme E2-like 206 +/- 22 244 +/- 17 285 +/- 21 283 +/- 11 0.023915 0.2131659 -0.910342 0.2155643 0.5593932 UGCG UDP-glucose ceramide glucosyltransferase 1454 +/- 63 1024 +/- 64 1638 +/- 69 1487 +/- 121 0.016063 -0.000733 -0.318254 0.0010664 0.4295965 UHMK1 U2AF homology motif (UHM) kinase 1 347 +/- 98 318 +/- 62 466 +/- 78 581 +/- 90 0.037222 -0.80937 0.362263 0.6089661 0.4656584 UNC13A unc-13 homolog A (C. elegans) 4375 +/- 548 3490 +/- 308 4914 +/- 292 4433 +/- 101 0.015851 -0.19733 -0.169391 0.2125362 0.4315568 UNC13C unc-13 homolog C (C. elegans) 555 +/- 100 536 +/- 112 1134 +/- 113 1508 +/- 113 6.49E-05 -0.904622 0.044441 0.9489766 0.1532116 UNC5A unc-5 homolog A (C. elegans) 2942 +/- 415 2588 +/- 308 4685 +/- 192 4537 +/- 141 6.4E-06 -0.509057 -0.553178 0.4376494 0.7312857 UNQ9391 tryptophan/serine protease 230 +/- 32 230 +/- 28 376 +/- 68 330 +/- 64 0.001186 0.9995504 -0.638373 0.9957608 0.9262423 URG4 Up-regulated gene 4 143 +/- 13 185 +/- 39 207 +/- 17 225 +/- 44 0.002523 0.3477214 0.722383 0.1728096 0.5130241 USF2 upstream transcription factor 2, c-fos interacting 1751 +/- 120 1856 +/- 103 2246 +/- 71 2251 +/- 91 0.000256 0.5198199 0.963564 0.7121665 0.3098994 USP1 ubiquitin specific peptidase 1 246 +/- 23 298 +/- 23 324 +/- 22 333 +/- 20 0.04091 0.13719 0.749478 0.0504862 0.4958402 USP11 ubiquitin specific peptidase 11 5050 +/- 405 5345 +/- 377 6253 +/- 122 6562 +/- 395 0.002376 0.6063314 0.490697 0.8201948 0.0599795 USP22 ubiquitin specific peptidase 22 6620 +/- 505 6082 +/- 535 7734 +/- 576 7141 +/- 522 0.000709 -0.481633 -0.465468 0.6597791 0.6400643 USP28 ubiquitin specific peptidase 28 309 +/- 28 362 +/- 44 450 +/- 38 451 +/- 43 0.00054 0.3286532 0.985795 0.0764637 0.6491161 USP31 ubiquitin specific peptidase 31 360 +/- 19 436 +/- 27 499 +/- 36 687 +/- 45 0.000805 0.0458499 0.011486 0.1335057 0.1219157 USP33 ubiquitin specific peptidase 33 626 +/- 44 814 +/- 106 896 +/- 94 889 +/- 102 0.002988 0.1474969 -0.956814 0.0288263 0.3352423 USP54 ubiquitin specific peptidase 54 290 +/- 19 331 +/- 30 392 +/- 38 347 +/- 31 0.046401 0.2828821 -0.38414 0.4232834 0.1444715 UST uronyl-2-sulfotransferase 78 +/- 24 140 +/- 44 249 +/- 39 293 +/- 39 0.000857 0.2434981 0.450922 0.5862095 0.8619314 UTP18 UTP18, small subunit (SSU) processome component, homolog (yeast) 599 +/- 58 413 +/- 38 673 +/- 50 479 +/- 70 0.043722 -0.026658 -0.055462 0.1362504 0.3656743 UXS1 UDP-glucuronate decarboxylase 1 801 +/- 71 662 +/- 61 974 +/- 34 857 +/- 59 0.001719 -0.170227 -0.133849 0.1819497 0.4824605 VAMP2 vesicle-associated membrane protein 2 (synaptobrevin 2) 12990 +/- 255 12003 +/- 489 16322 +/- 332 16183 +/- 451 2.51E-07 -0.113853 -0.811338 0.0068253 0.6205985 VASH1 Vasohibin 1 535 +/- 16 744 +/- 63 1011 +/- 90 999 +/- 53 2.52E-05 0.0198306 -0.908355 0.071398 0.9152143 VEGFB vascular endothelial growth factor B 190 +/- 21 174 +/- 25 345 +/- 11 376 +/- 33 9.22E-06 -0.622574 0.410876 0.6961231 0.3428105 VGCNL1 voltage gated channel like 1 82 +/- 17 96 +/- 16 158 +/- 21 136 +/- 15 0.012019 0.5640064 -0.416453 0.4779032 0.429056 VPS13A vacuolar protein sorting 13 homolog A (S. cerevisiae) 1676 +/- 195 1449 +/- 92 2198 +/- 82 1927 +/- 110 4.42E-05 -0.326019 -0.08481 0.4163498 0.3039349 VPS37B vacuolar protein sorting 37 homolog B (S. cerevisiae) 1798 +/- 95 1607 +/- 186 2109 +/- 138 1793 +/- 92 0.045411 -0.389358 -0.091311 0.3721637 0.2454975 VPS37D vacuolar protein sorting 37 homolog D (S. cerevisiae) 519 +/- 26 502 +/- 35 638 +/- 37 616 +/- 49 0.018984 -0.717953 -0.737571 0.3614872 0.4340391 VPS41 vacuolar protein sorting 41 (yeast) 837 +/- 38 914 +/- 46 896 +/- 48 1005 +/- 64 0.004625 0.2251202 0.209433 0.2144679 0.2704232

S2-genes differentially expressed across region.xls Page 33 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI VPS52 vacuolar protein sorting 52 (S. cerevisiae) 2096 +/- 88 2181 +/- 130 2255 +/- 63 2679 +/- 158 0.004678 0.6022707 0.052527 0.8677483 0.0234175 WBP11 WW domain binding protein 11 3554 +/- 268 3189 +/- 119 3778 +/- 79 3610 +/- 55 0.035057 -0.254967 -0.11805 0.1339353 0.0502493 WBP5 WW domain binding protein 5 2443 +/- 318 2531 +/- 139 3156 +/- 84 2951 +/- 97 0.008611 0.8083123 -0.146902 0.7072396 0.2504388 WBSCR17 Williams-Beuren syndrome chromosome region 17 3846 +/- 222 2759 +/- 329 4476 +/- 273 3946 +/- 239 0.003811 -0.023533 -0.178057 0.0095031 0.5398113 WDFY3 WD repeat and FYVE domain containing 3 2635 +/- 304 2528 +/- 167 3233 +/- 258 3486 +/- 170 0.009028 -0.764655 0.435442 0.9916405 0.4114281 WDR39 WD repeat domain 39 633 +/- 28 580 +/- 22 791 +/- 33 754 +/- 48 0.000392 -0.169727 -0.546174 0.2151565 0.8293635 WDR45L WDR45-like 610 +/- 39 568 +/- 18 726 +/- 26 684 +/- 24 0.000238 -0.366247 -0.264986 0.2734846 0.8377011 WDR67 WD repeat domain 67 408 +/- 52 473 +/- 25 518 +/- 47 573 +/- 44 0.010942 0.2989411 0.413276 0.2648955 0.6170061 WDSUB1 WD repeat, sterile alpha motif and U-box domain containing 1 515 +/- 23 464 +/- 34 586 +/- 34 515 +/- 28 0.035757 -0.258457 -0.138048 0.2613205 0.455581 WFIKKN1 WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 171 +/- 26 162 +/- 21 244 +/- 22 211 +/- 20 0.002521 -0.788262 -0.300441 0.5165338 0.3147883 WHDC1 WAS protein homology region 2 domain containing 1 276 +/- 18 251 +/- 22 322 +/- 19 263 +/- 13 0.001236 -0.402533 -0.033976 0.2380032 0.061158 WIZ widely-interspaced zinc finger motifs 1361 +/- 65 1217 +/- 28 1483 +/- 47 1401 +/- 85 0.004563 -0.082209 -0.42558 0.0485294 0.4006016 WNT2B wingless-type MMTV integration site family, member 2B 141 +/- 11 118 +/- 11 163 +/- 9 142 +/- 19 0.042071 -0.168066 -0.362651 0.0346482 0.1807721 WNT3 wingless-type MMTV integration site family, member 3 355 +/- 36 256 +/- 29 472 +/- 51 342 +/- 26 0.001763 -0.059504 -0.055886 0.1603254 0.1346164 WRNIP1 Werner helicase interacting protein 1 2354 +/- 101 2186 +/- 134 3033 +/- 152 2971 +/- 26 4.26E-05 -0.340677 -0.698637 0.3712669 0.7585791 WSB2 WD repeat and SOCS box-containing 2 7569 +/- 145 7300 +/- 367 8524 +/- 288 8313 +/- 399 0.001263 -0.519115 -0.679261 0.2104443 0.4683081 WTAP Wilms tumor 1 associated protein 519 +/- 31 710 +/- 21 683 +/- 41 771 +/- 66 0.012206 0.0006447 0.292143 0.0003647 0.0433612 WWC3 WWC family member 3 680 +/- 28 741 +/- 29 739 +/- 37 775 +/- 48 0.034064 0.1617262 0.569867 0.0872943 0.5981949 XPR1 Xenotropic and polytropic retrovirus receptor 1829 +/- 201 1625 +/- 117 2672 +/- 206 2242 +/- 152 0.005698 -0.405808 -0.128756 0.5144049 0.395138 XRCC6BP1XRCC6 binding protein 1 301 +/- 20 313 +/- 32 419 +/- 27 409 +/- 17 0.000586 0.7486826 -0.760635 0.429831 0.9985575 XRN1 5'-3' exoribonuclease 1 314 +/- 23 346 +/- 18 416 +/- 18 408 +/- 28 0.003161 0.3058441 -0.80817 0.4214337 0.4761623 YIPF2 Yip1 domain family, member 2 771 +/- 39 783 +/- 85 966 +/- 39 1017 +/- 43 0.002247 0.9009127 0.405569 0.8341146 0.138297 YIPF5 Yip1 domain family, member 5 2776 +/- 72 2388 +/- 136 2883 +/- 102 2798 +/- 74 0.02751 -0.037028 -0.516929 0.0084887 0.633725 YRDC yrdC domain containing (E. coli) 396 +/- 15 305 +/- 14 466 +/- 9 394 +/- 30 0.001004 -0.001409 -0.075638 0.0027356 0.4238058 YTHDF2 YTH domain family, member 2 2059 +/- 97 1665 +/- 58 2492 +/- 66 2178 +/- 98 0.00012 -0.007866 -0.031414 0.1128308 0.432849 YWHAG tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polyp 20235 +/- 783 18812 +/- 635 20710 +/- 678 21437 +/- 756 0.028379 -0.189738 0.492819 0.1535006 0.9845022 ZA20D3 Zinc finger, A20 domain containing 3 171 +/- 10 222 +/- 21 222 +/- 14 221 +/- 13 0.019293 0.0655536 -0.940827 0.0085964 0.7023625 ZAK sterile alpha motif and leucine zipper containing kinase AZK 523 +/- 34 457 +/- 37 703 +/- 101 533 +/- 46 0.022417 -0.215943 -0.169083 0.2238292 0.3289735 ZBED1 zinc finger, BED-type containing 1 1205 +/- 69 1265 +/- 97 1434 +/- 62 1473 +/- 72 0.020184 0.6245763 0.695318 0.9299459 0.427471 ZBTB2 zinc finger and BTB domain containing 2 196 +/- 4 189 +/- 6 236 +/- 8 210 +/- 10 8.85E-05 -0.426691 -0.078207 0.7487008 0.5458288 ZBTB20 Zinc finger and BTB domain containing 20 73 +/- 9 98 +/- 8 119 +/- 14 127 +/- 11 0.000232 0.0645116 0.623125 0.0108026 0.5206635 ZBTB33 zinc finger and BTB domain containing 33 2068 +/- 190 1926 +/- 93 2266 +/- 50 2340 +/- 47 0.007219 -0.520695 0.305785 0.5668458 0.3407075 ZBTB6 zinc finger and BTB domain containing 6 241 +/- 10 243 +/- 12 301 +/- 12 279 +/- 10 0.000162 0.9477737 -0.184121 0.7667897 0.0544392 ZBTB7A zinc finger and BTB domain containing 7A 1920 +/- 64 1641 +/- 79 2555 +/- 279 2188 +/- 127 0.001221 -0.021473 -0.27096 0.1108262 0.5977751 ZBTB8 zinc finger and BTB domain containing 8 163 +/- 26 200 +/- 13 226 +/- 23 248 +/- 21 0.007398 0.2512714 0.501441 0.5335853 0.7457664 ZC3H14 zinc finger CCCH-type containing 14 1650 +/- 138 1245 +/- 70 1782 +/- 55 1603 +/- 67 0.006636 -0.033219 -0.072998 0.0842893 0.4758872 ZC3H7A zinc finger CCCH-type containing 7A 323 +/- 29 364 +/- 14 362 +/- 23 412 +/- 17 0.0263 0.2413865 0.11879 0.3548957 0.297632 ZCCHC17 zinc finger, CCHC domain containing 17 904 +/- 39 1024 +/- 47 1131 +/- 49 1021 +/- 52 0.016006 0.0762725 -0.162783 0.4129188 0.0337418 ZCCHC4 zinc finger, CCHC domain containing 4 129 +/- 15 142 +/- 11 179 +/- 11 158 +/- 13 0.003647 0.5316745 -0.255222 0.2284574 0.3419854 ZCCHC9 zinc finger, CCHC domain containing 9 897 +/- 53 848 +/- 32 1071 +/- 46 942 +/- 45 0.022511 -0.454538 -0.074569 0.8930571 0.2459693 ZDHHC1 zinc finger, DHHC-type containing 1 157 +/- 21 200 +/- 25 235 +/- 16 257 +/- 16 0.004213 0.2159697 0.347212 0.2799185 0.9170135 ZDHHC15 zinc finger, DHHC-type containing 15 332 +/- 20 338 +/- 18 397 +/- 14 362 +/- 14 0.009873 0.8314976 -0.108421 0.9000848 0.0795048 ZDHHC18 zinc finger, DHHC-type containing 18 223 +/- 14 215 +/- 9 266 +/- 15 299 +/- 19 0.000597 -0.625972 0.204311 0.9259997 0.1438175 ZDHHC3 zinc finger, DHHC-type containing 3 327 +/- 26 276 +/- 38 418 +/- 25 375 +/- 15 0.006707 -0.297697 -0.168635 0.201915 0.1320413 ZDHHC8 zinc finger, DHHC-type containing 8 483 +/- 29 442 +/- 16 630 +/- 45 536 +/- 23 0.006318 -0.254056 -0.103633 0.3887959 0.2342682

S2-genes differentially expressed across region.xls Page 34 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ZF HCF-binding transcription factor Zhangfei 481 +/- 22 590 +/- 26 608 +/- 20 692 +/- 20 0.000708 0.0098783 0.016805 0.0079603 0.3500007 ZFP30 zinc finger protein 30 homolog (mouse) 507 +/- 14 543 +/- 38 615 +/- 45 648 +/- 71 0.003177 0.4017811 0.710667 0.0485218 0.3973963 ZFP90 zinc finger protein 90 homolog (mouse) 3528 +/- 279 4260 +/- 161 3870 +/- 345 4323 +/- 157 0.025622 0.0525751 0.270959 0.0915682 0.4421446 ZFYVE1 zinc finger, FYVE domain containing 1 204 +/- 23 229 +/- 17 263 +/- 13 296 +/- 17 0.013793 0.4098305 0.171816 0.6915038 0.3958799 ZFYVE20 zinc finger, FYVE domain containing 20 102 +/- 17 70 +/- 6 122 +/- 11 112 +/- 5 0.023073 -0.121761 -0.455929 0.0874595 0.4969319 ZFYVE27 zinc finger, FYVE domain containing 27 2177 +/- 96 2097 +/- 162 2316 +/- 52 2309 +/- 109 0.040981 -0.68373 -0.952885 0.1717035 0.2790218 ZFYVE28 zinc finger, FYVE domain containing 28 425 +/- 19 395 +/- 10 596 +/- 35 569 +/- 51 0.000193 -0.210957 -0.667875 0.2468468 0.9875766 ZMYM3 zinc finger, MYM-type 3 281 +/- 15 293 +/- 13 354 +/- 15 392 +/- 20 4.39E-05 0.5681973 0.174397 0.9456254 0.4530916 ZMYM4 zinc finger, MYM-type 4 607 +/- 22 589 +/- 35 721 +/- 22 682 +/- 13 0.000205 -0.676327 -0.159697 0.5478719 0.0538551 ZNF114 zinc finger protein 114 107 +/- 3 125 +/- 14 135 +/- 12 126 +/- 11 0.020501 0.2593782 -0.598494 0.3699002 0.4357346 ZNF12 zinc finger protein 12 809 +/- 59 700 +/- 10 934 +/- 44 828 +/- 54 0.008745 -0.122813 -0.165469 0.1224268 0.4543277 ZNF131 Zinc finger protein 131 (clone pHZ-10) 124 +/- 18 238 +/- 29 160 +/- 22 267 +/- 25 0.016536 0.0100522 0.011395 0.1261566 0.0668083 ZNF189 zinc finger protein 189 1618 +/- 207 1411 +/- 158 1863 +/- 46 1837 +/- 69 0.018596 -0.446162 -0.756508 0.5814633 0.8002239 ZNF19 zinc finger protein 19 148 +/- 17 107 +/- 13 190 +/- 15 173 +/- 14 0.020289 -0.087649 -0.438561 0.3217213 0.43376 ZNF195 zinc finger protein 195 327 +/- 48 323 +/- 19 379 +/- 48 349 +/- 14 0.042791 -0.938495 -0.568958 0.7849727 0.4991122 ZNF197 zinc finger protein 197 370 +/- 35 200 +/- 10 492 +/- 28 326 +/- 18 1.85E-05 -0.00368 -0.000982 0.0070485 0.01695 ZNF219 zinc finger protein 219 720 +/- 46 791 +/- 57 886 +/- 23 918 +/- 33 0.000566 0.3616298 0.449246 0.84916 0.8722246 ZNF221 zinc finger protein 221 175 +/- 14 164 +/- 12 202 +/- 14 195 +/- 17 0.03668 -0.55094 -0.770858 0.8516415 0.5586447 ZNF238 zinc finger protein 238 7398 +/- 390 6014 +/- 471 8244 +/- 127 6592 +/- 730 0.049554 -0.047964 -0.08576 0.1522323 0.5300615 ZNF25 zinc finger protein 25 1239 +/- 41 1519 +/- 51 1305 +/- 50 1653 +/- 73 0.004793 0.0018547 0.00525 0.0858856 0.0659915 ZNF267 zinc finger protein 267 177 +/- 18 186 +/- 22 250 +/- 8 228 +/- 16 0.00191 0.7579929 -0.284428 0.4325489 0.6491789 ZNF268 zinc finger protein 268 176 +/- 5 187 +/- 9 248 +/- 13 245 +/- 10 6.97E-05 0.3168509 -0.866404 0.1821338 0.6363664 ZNF278 zinc finger protein 278 239 +/- 11 277 +/- 12 317 +/- 19 300 +/- 16 0.019184 0.0427149 -0.486719 0.0601858 0.7465071 ZNF282 zinc finger protein 282 587 +/- 32 638 +/- 26 745 +/- 26 698 +/- 20 0.00278 0.2408864 -0.172911 0.4845465 0.3355149 ZNF292 zinc finger protein 292 587 +/- 17 672 +/- 38 793 +/- 46 717 +/- 48 0.019011 0.0801015 -0.289456 0.0765608 0.6619526 ZNF30 zinc finger protein 30 183 +/- 21 193 +/- 20 252 +/- 36 225 +/- 26 0.001603 0.7322227 -0.558786 0.1122953 0.6091916 ZNF306 Zinc finger protein 306 118 +/- 16 157 +/- 18 180 +/- 18 182 +/- 18 0.005107 0.1371985 0.951361 0.2601777 0.6833357 ZNF323 zinc finger protein 323 162 +/- 15 187 +/- 22 236 +/- 13 296 +/- 17 0.001103 0.368404 0.023073 0.6848203 0.0231175 ZNF331 zinc finger protein 331 749 +/- 77 878 +/- 49 955 +/- 35 1047 +/- 27 0.001112 0.193887 0.067534 0.1724808 0.028373 ZNF347 zinc finger protein 347 126 +/- 11 178 +/- 7 162 +/- 16 181 +/- 15 0.030671 0.0042757 0.409142 0.0166876 0.8325214 ZNF358 zinc finger protein 358 556 +/- 13 684 +/- 43 872 +/- 81 877 +/- 12 0.000402 0.028655 0.953891 0.0864711 0.9796446 ZNF364 zinc finger protein 364 1966 +/- 224 1611 +/- 142 2340 +/- 263 1915 +/- 122 0.000806 -0.215149 -0.185383 0.3870021 0.4582997 ZNF385 zinc finger protein 385 773 +/- 60 764 +/- 62 1254 +/- 67 1190 +/- 42 4.87E-06 -0.916787 -0.443772 0.4341433 0.2357291 ZNF390 zinc finger protein 390 168 +/- 21 180 +/- 10 217 +/- 15 212 +/- 9 0.014646 0.6272967 -0.758499 0.7023835 0.7942171 ZNF430 zinc finger protein 430 359 +/- 59 450 +/- 57 481 +/- 70 519 +/- 74 0.027438 0.2894408 0.715109 0.4684203 0.9933476 ZNF434 zinc finger protein 434 229 +/- 27 279 +/- 34 429 +/- 30 416 +/- 54 0.000965 0.2723783 -0.832146 0.1064989 0.8937655 ZNF435 zinc finger protein 435 110 +/- 9 147 +/- 5 143 +/- 12 160 +/- 5 0.033286 0.0094135 0.248189 0.0044561 0.7177566 ZNF436 zinc finger protein 436 845 +/- 77 754 +/- 83 1185 +/- 39 1061 +/- 36 0.000267 -0.443221 -0.045877 0.4482469 0.0157843 ZNF444 zinc finger protein 444 641 +/- 67 642 +/- 25 770 +/- 39 731 +/- 38 0.01247 0.9900256 -0.478501 0.8013133 0.6119273 ZNF447 zinc finger protein 447 6480 +/- 176 6013 +/- 367 8138 +/- 192 8323 +/- 247 8.12E-07 -0.287621 0.57145 0.0133939 0.6867979 ZNF45 zinc finger protein 45 279 +/- 18 336 +/- 17 332 +/- 22 400 +/- 18 0.001286 0.0495013 0.045354 0.2410449 0.225943 ZNF452 zinc finger protein 452 148 +/- 21 151 +/- 15 209 +/- 9 177 +/- 7 0.014954 0.8964179 -0.023525 0.9426293 0.0763942 ZNF493 zinc finger protein 493 153 +/- 21 181 +/- 15 179 +/- 24 212 +/- 8 0.001711 0.3042095 0.226304 0.5064487 0.3033026 ZNF501 zinc finger protein 501 137 +/- 4 217 +/- 10 206 +/- 8 206 +/- 8 0.044857 0.0002728 -0.95366 0.0016356 0.8863999 ZNF502 zinc finger protein 502 356 +/- 27 410 +/- 20 386 +/- 16 431 +/- 16 0.037923 0.1447858 0.072678 0.5431746 0.0974448

S2-genes differentially expressed across region.xls Page 35 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ZNF503 zinc finger protein 503 989 +/- 27 822 +/- 31 1260 +/- 34 1165 +/- 60 1.25E-06 -0.002239 -0.214355 0.0017121 0.1866212 ZNF555 zinc finger protein 555 362 +/- 20 380 +/- 17 437 +/- 27 378 +/- 24 0.04619 0.516341 -0.136688 0.5125201 0.1286823 ZNF559 Zinc finger protein 559 116 +/- 9 94 +/- 8 149 +/- 18 118 +/- 7 0.005537 -0.095442 -0.166653 0.2433891 0.4638124 ZNF566 zinc finger protein 566 228 +/- 18 246 +/- 30 336 +/- 14 336 +/- 34 2.04E-05 0.6175617 0.993307 0.2325645 0.5332713 ZNF570 zinc finger protein 570 94 +/- 6 112 +/- 10 149 +/- 4 137 +/- 7 0.000537 0.1720326 -0.145391 0.2220687 0.9296035 ZNF573 zinc finger protein 573 187 +/- 34 144 +/- 13 251 +/- 30 152 +/- 28 0.039697 -0.279536 -0.039265 0.5620131 0.4317854 ZNF575 zinc finger protein 575 345 +/- 35 284 +/- 22 392 +/- 19 412 +/- 13 0.019584 -0.171246 0.412578 0.0419573 0.1456269 ZNF580 zinc finger protein 580 977 +/- 46 933 +/- 22 1370 +/- 34 1366 +/- 51 3.43E-06 -0.415504 -0.952135 0.5930736 0.7841005 ZNF586 zinc finger protein 586 316 +/- 21 232 +/- 26 438 +/- 20 279 +/- 34 0.00204 -0.031863 -0.005803 0.0353043 0.0017489 ZNF607 zinc finger protein 607 223 +/- 9 235 +/- 18 291 +/- 11 257 +/- 17 0.001841 0.5705562 -0.126544 0.4880873 0.1250607 ZNF625 zinc finger protein 625 105 +/- 8 110 +/- 9 122 +/- 14 113 +/- 8 0.04849 0.6801533 -0.600199 0.4741532 0.8616622 ZNF629 zinc finger protein 629 637 +/- 43 636 +/- 24 753 +/- 28 734 +/- 21 0.003072 -0.991827 -0.587096 0.8007429 0.7441857 ZNF644 zinc finger protein 644 199 +/- 32 259 +/- 36 281 +/- 20 341 +/- 52 0.046895 0.236237 0.326765 0.2638957 0.5021827 ZNF651 zinc finger protein 651 305 +/- 22 361 +/- 29 396 +/- 9 412 +/- 43 0.044273 0.1526737 0.740549 0.0634426 0.6643048 ZNF658 zinc finger protein 658 201 +/- 19 171 +/- 8 278 +/- 14 206 +/- 22 0.013475 -0.206711 -0.026069 0.2586914 0.1252363 ZNF672 zinc finger protein 672 159 +/- 20 192 +/- 23 219 +/- 8 245 +/- 24 0.008891 0.2914762 0.373429 0.3790093 0.6438691 ZNF75 zinc finger protein 75 (D8C6) 118 +/- 9 171 +/- 11 232 +/- 23 191 +/- 11 0.013173 0.0039383 -0.160314 0.0464456 0.0901358 ZNF76 zinc finger protein 76 (expressed in testis) 527 +/- 36 550 +/- 37 616 +/- 36 580 +/- 23 0.046598 0.6650163 -0.429049 0.6548385 0.7129831 ZNHIT4 zinc finger, HIT type 4 320 +/- 15 362 +/- 24 394 +/- 18 402 +/- 35 0.024347 0.1659424 0.831873 0.1170649 0.4533148 ZYG11B Zyg-11 homolog B (C. elegans) 783 +/- 95 1096 +/- 114 916 +/- 54 1329 +/- 49 0.004684 0.0627527 0.000304 0.2823152 0.0091151 Overexpressed in DG AACS acetoacetyl-CoA synthetase 1751 +/- 102 1591 +/- 86 1523 +/- 75 1481 +/- 49 -0.016233 -0.258778 -0.648834 0.128366 0.6137267 AADAT aminoadipate aminotransferase 271 +/- 29 237 +/- 19 145 +/- 17 143 +/- 19 -0.000335 -0.370529 -0.959854 0.3032134 0.7254791 AASDH 2-aminoadipic 6-semialdehyde dehydrogenase 587 +/- 19 771 +/- 21 501 +/- 29 664 +/- 26 -6.83E-05 8.644E-05 0.002318 0.0046175 0.0347906 AATF apoptosis antagonizing transcription factor 389 +/- 16 435 +/- 24 350 +/- 17 373 +/- 10 -0.013171 0.1575421 0.265816 0.0173386 0.1137768 ABCA2 ATP-binding cassette, sub-family A (ABC1), member 2 3508 +/- 582 4558 +/- 624 2835 +/- 141 3151 +/- 200 -0.044031 0.2467877 0.235291 0.0806423 0.2178105 ABCA8 ATP-binding cassette, sub-family A (ABC1), member 8 492 +/- 90 430 +/- 93 322 +/- 37 285 +/- 38 -0.014315 -0.645006 -0.50183 0.7149486 0.8450982 ABCC4 ATP-binding cassette, sub-family C (CFTR/MRP), member 4 437 +/- 32 380 +/- 18 322 +/- 22 284 +/- 35 -0.003298 -0.154478 -0.389004 0.4768687 0.2189396 ABCC5 ATP-binding cassette, sub-family C (CFTR/MRP), member 5 882 +/- 71 833 +/- 12 721 +/- 31 739 +/- 53 -0.005957 -0.522499 0.775559 0.6652941 0.6341033 ABCD4 ATP-binding cassette, sub-family D (ALD), member 4 223 +/- 61 147 +/- 28 162 +/- 33 99 +/- 17 -0.013608 -0.300099 -0.139387 0.4001925 0.211957 ABCG2 ATP-binding cassette, sub-family G (WHITE), member 2 437 +/- 47 523 +/- 31 335 +/- 10 398 +/- 11 -0.001844 0.1582204 0.002045 0.1826101 0.0544598 ABHD10 abhydrolase domain containing 10 2354 +/- 67 2239 +/- 127 1737 +/- 50 1834 +/- 41 -0.000188 -0.450627 0.165473 0.2208405 0.0744561 ABHD4 abhydrolase domain containing 4 719 +/- 43 630 +/- 34 585 +/- 27 564 +/- 36 -0.001432 -0.134428 -0.651686 0.2115173 0.3011896 ABHD7 abhydrolase domain containing 7 227 +/- 36 201 +/- 30 140 +/- 21 163 +/- 15 -0.004116 -0.590751 0.400479 0.2824869 0.4086488 ABL1 v-abl Abelson murine leukemia viral oncogene homolog 1 1405 +/- 84 1379 +/- 70 1223 +/- 31 1231 +/- 20 -0.008732 -0.81665 0.834521 0.8396622 0.481535 ABR active BCR-related gene 4665 +/- 282 4223 +/- 241 3977 +/- 225 3991 +/- 129 -0.011536 -0.261566 0.959197 0.0809025 0.9587233 ABTB2 Ankyrin repeat and BTB (POZ) domain containing 2 654 +/- 122 485 +/- 39 505 +/- 32 342 +/- 18 -0.030184 -0.236531 -0.002265 0.5021444 0.0832426 ACAA2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) 589 +/- 56 854 +/- 62 529 +/- 31 645 +/- 33 -0.03897 0.0097889 0.030153 0.000594 0.1078687 ACADS acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain 311 +/- 31 346 +/- 34 261 +/- 15 265 +/- 24 -0.021818 0.4642014 0.876887 0.2333204 0.3001278 ACAT1 acetyl-Coenzyme A acetyltransferase 1 (acetoacetyl Coenzyme A thiolase) 4099 +/- 188 3515 +/- 106 3250 +/- 137 3238 +/- 137 -0.007467 -0.027046 -0.954273 0.1128106 0.9705821 ACBD5 acyl-Coenzyme A binding domain containing 5 4356 +/- 369 4915 +/- 354 3761 +/- 224 3927 +/- 60 -0.003678 0.3001289 0.503026 0.1545917 0.3403616 ACCN1 amiloride-sensitive cation channel 1, neuronal (degenerin) 1925 +/- 136 1421 +/- 164 1179 +/- 59 900 +/- 42 -0.000254 -0.040656 -0.004392 0.0273253 0.0121481 ACOT7 acyl-CoA thioesterase 7 4567 +/- 119 4226 +/- 238 3764 +/- 158 3740 +/- 137 -0.000176 -0.239117 -0.91357 0.0443048 0.7733545 ACOT8 acyl-CoA thioesterase 8 651 +/- 34 662 +/- 39 571 +/- 17 601 +/- 28 -0.023548 0.8362974 0.403548 0.3351409 0.1514284 ACOT9 acyl-CoA thioesterase 9 370 +/- 33 344 +/- 40 284 +/- 9 218 +/- 20 -0.001741 -0.63831 -0.024981 0.6715461 0.2839666

S2-genes differentially expressed across region.xls Page 36 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ACP6 acid phosphatase 6, lysophosphatidic 881 +/- 49 984 +/- 51 742 +/- 28 837 +/- 8 -0.002935 0.171551 0.018151 0.0355159 0.0710112 ACPL2 acid phosphatase-like 2 2269 +/- 344 1642 +/- 129 1273 +/- 65 1279 +/- 73 -0.016713 -0.135982 0.955883 0.0922752 0.8212643 ACSS1 acyl-CoA synthetase short-chain family member 1 229 +/- 21 311 +/- 28 196 +/- 16 228 +/- 23 -0.013777 0.0442198 0.295382 0.092173 0.4151929 ACTL6A actin-like 6A 309 +/- 22 369 +/- 42 267 +/- 20 291 +/- 12 -0.020727 0.2424735 0.356269 0.0266745 0.3532226 ADAM10 ADAM metallopeptidase domain 10 1557 +/- 99 1808 +/- 84 1420 +/- 70 1612 +/- 46 -0.053577 0.0837127 0.049117 0.0627916 0.3649355 ADAMTS17ADAM metallopeptidase with thrombospondin type 1 motif, 17 113 +/- 10 126 +/- 15 89 +/- 6 97 +/- 13 -0.030633 0.4731131 0.544571 0.1948285 0.2868528 ADCK1 aarF domain containing kinase 1 478 +/- 32 485 +/- 25 408 +/- 25 400 +/- 26 -0.002745 0.8668716 -0.83027 0.5364103 0.5037556 ADCY5 adenylate cyclase 5 782 +/- 43 764 +/- 28 636 +/- 14 627 +/- 29 -0.000126 -0.727758 -0.795005 0.7575292 0.1320195 ADCY7 adenylate cyclase 7 154 +/- 16 183 +/- 20 122 +/- 11 111 +/- 9 -0.002811 0.2882067 -0.444627 0.0573021 0.8758977 ADCY8 adenylate cyclase 8 (brain) 842 +/- 32 772 +/- 62 492 +/- 39 485 +/- 16 -1.27E-05 -0.346282 -0.874863 0.1549163 0.6633748 ADD1 adducin 1 (alpha) 4262 +/- 135 3716 +/- 140 3921 +/- 116 3527 +/- 163 -0.000231 -0.018613 -0.086088 0.0313122 0.2337141 ADH5 alcohol dehydrogenase 5 (class III), chi polypeptide 2880 +/- 119 2714 +/- 250 2505 +/- 94 2725 +/- 150 -0.001489 -0.566182 0.253363 0.0822124 0.7457064 ADIPOR2 adiponectin receptor 2 2814 +/- 343 3544 +/- 400 1735 +/- 118 2083 +/- 155 -0.001503 0.197116 0.112243 0.1105974 0.3560335 ADPRH ADP-ribosylarginine hydrolase 189 +/- 23 309 +/- 20 168 +/- 16 281 +/- 20 -0.015382 0.0030101 0.00234 0.0028139 0.0090525 ADSS adenylosuccinate synthase 1086 +/- 45 1042 +/- 80 888 +/- 50 878 +/- 45 -8.9E-05 -0.637651 -0.884415 0.8606578 0.6521034 ADSSL1 adenylosuccinate synthase like 1 613 +/- 114 708 +/- 114 483 +/- 48 459 +/- 46 -0.015538 0.5705081 -0.719204 0.1866972 0.7457888 AEBP1 AE binding protein 1 344 +/- 61 279 +/- 23 185 +/- 45 234 +/- 26 -0.008481 -0.357009 0.381106 0.1734807 0.6822614 AFAP actin filament associated protein 2123 +/- 453 1801 +/- 181 675 +/- 94 685 +/- 54 -0.00094 -0.531267 0.926429 0.3430637 0.9160407 AGA aspartylglucosaminidase 134 +/- 12 153 +/- 15 116 +/- 11 120 +/- 16 -0.019452 0.3560886 0.827171 0.1060341 0.6355566 AGXT2L1 alanine-glyoxylate aminotransferase 2-like 1 3918 +/- 414 5586 +/- 247 3574 +/- 289 4607 +/- 398 -0.017396 0.0082839 0.070131 0.0534143 0.1478785 AHSA1 AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) 3672 +/- 96 3351 +/- 98 3165 +/- 122 3121 +/- 103 -0.010338 -0.04181 -0.788981 0.0745863 0.7081707 AIF1 allograft inflammatory factor 1 304 +/- 36 332 +/- 38 246 +/- 22 256 +/- 20 -0.032217 0.6062468 0.740217 0.2232582 0.5621515 AIM1 absent in melanoma 1 368 +/- 97 238 +/- 44 114 +/- 15 175 +/- 22 -0.04556 -0.265509 0.050898 0.4169037 0.3555645 AKNA AT-hook transcription factor 176 +/- 8 199 +/- 22 114 +/- 11 139 +/- 9 -0.000767 0.3725376 0.100771 0.3327064 0.0792742 AKR1A1 aldo-keto reductase family 1, member A1 (aldehyde reductase) 1965 +/- 241 2348 +/- 190 1513 +/- 61 1657 +/- 96 -0.002833 0.2414578 0.246697 0.3894574 0.8508787 AKR1C1 aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alph 5920 +/- 823 6993 +/- 469 3173 +/- 424 3822 +/- 213 -7.24E-06 0.29023 0.212276 0.2995837 0.2755385 AKR7A2 aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) 1175 +/- 77 1379 +/- 69 853 +/- 46 1153 +/- 50 -0.000463 0.0781494 0.001841 0.3070654 0.0892846 AKT3 V-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) 4120 +/- 100 3342 +/- 250 3311 +/- 184 3045 +/- 90 -0.019216 -0.025143 -0.234272 0.0121962 0.4146659 ALAD aminolevulinate, delta-, dehydratase 192 +/- 24 250 +/- 23 165 +/- 13 200 +/- 16 -0.045111 0.1145861 0.127988 0.0348568 0.0100427 ALAS1 aminolevulinate, delta-, synthase 1 751 +/- 34 738 +/- 30 597 +/- 32 663 +/- 25 -6.43E-05 -0.77894 0.141671 0.399775 0.477872 ALCAM activated leukocyte cell adhesion molecule 756 +/- 67 849 +/- 78 726 +/- 67 711 +/- 70 -0.048273 0.3849278 -0.877535 0.0919495 0.4747488 ALDH3B1 aldehyde dehydrogenase 3 family, member B1 147 +/- 9 263 +/- 37 91 +/- 15 124 +/- 5 -0.002691 0.024391 0.084644 0.0284083 0.1508073 ALG14 asparagine-linked glycosylation 14 homolog (yeast) 546 +/- 44 655 +/- 63 425 +/- 42 588 +/- 69 -0.021244 0.1887698 0.085366 0.0494473 0.0235134 ALG2 asparagine-linked glycosylation 2 homolog (S. cerevisiae, alpha-1,3-mannosyltransferase 1345 +/- 98 1121 +/- 59 1178 +/- 47 1022 +/- 125 -0.046497 -0.085638 -0.293861 0.09534 0.0210985 ALG5 asparagine-linked glycosylation 5 homolog (S. cerevisiae, dolichyl-phosphate beta-glucos 601 +/- 24 662 +/- 22 529 +/- 14 623 +/- 25 -0.004397 0.089144 0.015616 0.0442575 0.0523303 ALG8 asparagine-linked glycosylation 8 homolog (S. cerevisiae, alpha-1,3-glucosyltransferase) 733 +/- 32 698 +/- 33 624 +/- 20 638 +/- 35 -0.010331 -0.457548 0.72987 0.5370396 0.1738882 ALK anaplastic lymphoma kinase (Ki-1) 251 +/- 35 294 +/- 23 159 +/- 20 184 +/- 14 -0.001162 0.3377061 0.323848 0.2777344 0.1256262 ALKBH7 alkB, alkylation repair homolog 7 (E. coli) 923 +/- 34 1035 +/- 62 775 +/- 18 786 +/- 21 -0.00099 0.1493891 0.696325 0.1490856 0.8886323 ALOX5AP arachidonate 5-lipoxygenase-activating protein 396 +/- 54 473 +/- 55 281 +/- 18 331 +/- 24 -0.010084 0.3371905 0.134169 0.3069445 0.6138821 ANAPC1 anaphase promoting complex subunit 1 942 +/- 48 810 +/- 32 776 +/- 30 736 +/- 25 -0.002459 -0.048252 -0.328608 0.0962622 0.410849 ANAPC11 APC11 anaphase promoting complex subunit 11 homolog (yeast) 1418 +/- 65 1478 +/- 46 1036 +/- 22 1185 +/- 40 -2.42E-05 0.4651226 0.015793 0.9601436 0.2593954 ANAPC4 anaphase promoting complex subunit 4 1110 +/- 148 1228 +/- 79 754 +/- 85 767 +/- 42 -0.000294 0.5018834 0.900382 0.6510049 0.7067331 ANAPC5 anaphase promoting complex subunit 5 3847 +/- 68 3641 +/- 158 3390 +/- 66 3482 +/- 121 -0.033691 -0.27225 0.527117 0.6152763 0.1928984 ANGEL1 angel homolog 1 (Drosophila) 990 +/- 89 1199 +/- 141 815 +/- 92 911 +/- 143 -0.010072 0.2428381 0.589213 0.5379547 0.5706761 ANKIB1 ankyrin repeat and IBR domain containing 1 1928 +/- 106 2973 +/- 269 1694 +/- 82 1917 +/- 65 -0.007949 0.009743 0.063334 9E-05 0.0839082

S2-genes differentially expressed across region.xls Page 37 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ANKRD12 ankyrin repeat domain 12 1799 +/- 76 1732 +/- 62 1476 +/- 41 1446 +/- 52 -2.63E-05 -0.51388 -0.66722 0.8296338 0.9189295 ANKRD13 ankyrin repeat domain 13 961 +/- 84 1099 +/- 32 779 +/- 24 905 +/- 49 -0.007945 0.1730586 0.062163 0.0705879 0.524255 ANKRD15 ankyrin repeat domain 15 2043 +/- 338 2719 +/- 167 1931 +/- 248 2257 +/- 101 -0.038817 0.1137291 0.265351 0.1394026 0.4901454 ANKRD20Aankyrin repeat domain 20 family, member A1 143 +/- 19 247 +/- 40 57 +/- 20 106 +/- 21 -0.004106 0.0508737 0.130608 0.0214372 0.1929429 ANKRD25 ankyrin repeat domain 25 477 +/- 50 637 +/- 71 372 +/- 41 460 +/- 26 -0.013238 0.1001724 0.106964 0.0382709 0.1581043 ANKRD28 ankyrin repeat domain 28 3418 +/- 127 3600 +/- 239 3181 +/- 93 3270 +/- 110 -0.049427 0.5230741 0.55216 0.2240239 0.5540591 ANKRD38 ankyrin repeat domain 38 211 +/- 21 250 +/- 30 170 +/- 17 182 +/- 22 -0.007404 0.3189819 0.677062 0.4691072 0.7174673 ANKRD46 ankyrin repeat domain 46 4646 +/- 144 4415 +/- 178 4103 +/- 199 4477 +/- 128 -0.031772 -0.336974 0.151229 0.095881 0.5963632 ANKRD50 ankyrin repeat domain 50 329 +/- 30 259 +/- 18 225 +/- 20 193 +/- 21 -0.001732 -0.083156 -0.294519 0.0364904 0.8501523 ANKS1A ankyrin repeat and sterile alpha motif domain containing 1A 908 +/- 65 891 +/- 68 654 +/- 52 594 +/- 13 -0.0003 -0.863369 -0.310129 0.9891414 0.4643054 ANLN anillin, actin binding protein (scraps homolog, Drosophila) 2264 +/- 473 3340 +/- 683 1196 +/- 209 1603 +/- 198 -0.011277 0.2279467 0.190942 0.0433415 0.1516347 ANP32B acidic (leucine-rich) nuclear phosphoprotein 32 family, member B 5261 +/- 630 6605 +/- 629 4342 +/- 135 4863 +/- 137 -0.020829 0.1620245 0.024182 0.065918 0.0327325 ANTXR1 anthrax toxin receptor 1 2619 +/- 129 2393 +/- 92 2291 +/- 97 2213 +/- 85 -0.01722 -0.189885 -0.560737 0.3574363 0.5253278 AP1S1 adaptor-related protein complex 1, sigma 1 subunit 4669 +/- 306 3964 +/- 224 3161 +/- 69 3298 +/- 116 -0.001375 -0.095771 0.347447 0.0284608 0.9469582 AP2B1 adaptor-related protein complex 2, beta 1 subunit 7691 +/- 354 5572 +/- 446 5527 +/- 121 4060 +/- 189 -0.000127 -0.004297 -0.000323 0.0287975 0.0273872 AP3M1 adaptor-related protein complex 3, mu 1 subunit 345 +/- 25 382 +/- 27 290 +/- 26 296 +/- 12 -0.014847 0.3293828 0.864174 0.0762015 0.8386735 AP3S1 adaptor-related protein complex 3, sigma 1 subunit 4609 +/- 211 3959 +/- 235 3561 +/- 127 3309 +/- 187 -0.000858 -0.067063 -0.299324 0.1956016 0.916877 APEH N-acylaminoacyl-peptide hydrolase 560 +/- 15 620 +/- 32 491 +/- 29 540 +/- 23 -0.009036 0.1320897 0.211972 0.7321269 0.5195361 APH1B anterior pharynx defective 1 homolog B (C. elegans) 308 +/- 18 294 +/- 22 231 +/- 11 272 +/- 18 -0.013807 -0.63656 0.089354 0.5775505 0.217942 APOA1BP apolipoprotein A-I binding protein 3444 +/- 146 3736 +/- 191 2627 +/- 74 3301 +/- 117 -0.000886 0.2539505 0.001861 0.2150369 0.0332909 APOD apolipoprotein D 1694 +/- 156 2698 +/- 413 1143 +/- 60 1879 +/- 176 -0.002071 0.0605189 0.011046 0.0199683 0.0099519 APOLD1 apolipoprotein L domain containing 1 405 +/- 51 331 +/- 34 309 +/- 40 287 +/- 19 -0.016736 -0.252973 -0.645961 0.6881354 0.6652725 APPBP2 amyloid beta precursor protein (cytoplasmic tail) binding protein 2 427 +/- 29 490 +/- 39 329 +/- 19 367 +/- 33 -0.003508 0.2249044 0.368394 0.114124 0.2279848 AQP11 aquaporin 11 454 +/- 20 431 +/- 34 389 +/- 9 423 +/- 26 -0.039894 -0.581801 0.275537 0.3538998 0.5710959 ARCN1 archain 1 3733 +/- 117 3567 +/- 79 3444 +/- 82 3322 +/- 114 -0.003342 -0.273316 -0.409748 0.6610527 0.9570511 ARG2 arginase, type II 521 +/- 31 499 +/- 51 443 +/- 30 455 +/- 21 -0.035714 -0.725526 0.752874 0.2416734 0.6399468 ARHGAP23Rho GTPase activating protein 23 2054 +/- 218 2294 +/- 232 1799 +/- 51 1763 +/- 76 -0.043482 0.468632 -0.704243 0.2860338 0.5431348 ARHGAP24Rho GTPase activating protein 24 136 +/- 12 186 +/- 20 108 +/- 10 127 +/- 11 -0.005389 0.0660111 0.233288 0.0346964 0.6347949 ARHGDIB Rho GDP dissociation inhibitor (GDI) beta 407 +/- 55 462 +/- 38 316 +/- 20 364 +/- 32 -0.030199 0.4306757 0.246602 0.4508659 0.3548889 ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7 3467 +/- 223 2866 +/- 102 2911 +/- 102 2790 +/- 61 -0.02838 -0.043674 -0.336368 0.0223283 0.410873 ARIH2 ariadne homolog 2 (Drosophila) 1295 +/- 30 1073 +/- 49 1029 +/- 41 1044 +/- 54 -0.015864 -0.004513 0.829441 0.1147651 0.7756638 ARL2 ADP-ribosylation factor-like 2 3128 +/- 228 3731 +/- 217 2370 +/- 109 2961 +/- 240 -0.000183 0.0849026 0.068544 0.0152728 0.0087737 ARL6IP4 ADP-ribosylation-like factor 6 interacting protein 4 4648 +/- 382 4150 +/- 266 3223 +/- 149 3173 +/- 184 -0.000148 -0.312604 -0.838356 0.4210307 0.576805 ARL9 ADP-ribosylation factor-like 9 194 +/- 38 201 +/- 22 130 +/- 19 133 +/- 11 -0.000764 0.8672757 0.882188 0.8031975 0.8307795 ARMCX3 armadillo repeat containing, X-linked 3 6123 +/- 136 5991 +/- 95 5672 +/- 212 5450 +/- 148 -0.017872 -0.446391 -0.41396 0.2480136 0.7252492 ARMET arginine-rich, mutated in early stage tumors 2227 +/- 120 1976 +/- 100 1929 +/- 85 1785 +/- 58 -0.037439 -0.141053 -0.195524 0.2220125 0.5383648 ARPC1A actin related protein 2/3 complex, subunit 1A, 41kDa 4084 +/- 587 3175 +/- 168 2942 +/- 129 2844 +/- 144 -0.047181 -0.188913 -0.625167 0.1674972 0.7157558 ARPC1B actin related protein 2/3 complex, subunit 1B, 41kDa 265 +/- 16 338 +/- 19 206 +/- 20 235 +/- 20 -0.001183 0.0141484 0.321994 0.0021732 0.7072972 ARPP-19 cyclic AMP phosphoprotein, 19 kD 4176 +/- 221 3910 +/- 221 3613 +/- 90 3636 +/- 130 -0.032804 -0.414751 0.890752 0.4210115 0.8026563 ARRDC2 arrestin domain containing 2 560 +/- 89 753 +/- 112 291 +/- 23 365 +/- 29 -0.000987 0.2086178 0.077614 0.0897362 0.0283749 ARSA arylsulfatase A 195 +/- 14 195 +/- 15 130 +/- 5 163 +/- 23 -0.001969 -0.998645 0.237997 0.6336815 0.2168226 ARVCF armadillo repeat gene deletes in velocardiofacial syndrome 824 +/- 112 914 +/- 35 724 +/- 56 724 +/- 24 -0.029073 0.4775261 -0.991083 0.4394348 0.5878608 ASAH1 N-acylsphingosine amidohydrolase (acid ceramidase) 1 8879 +/- 1417 7399 +/- 331 5610 +/- 412 6304 +/- 502 -0.03534 -0.351536 0.315616 0.3754098 0.3166588 ASL argininosuccinate lyase 747 +/- 41 753 +/- 44 589 +/- 18 598 +/- 23 -0.000201 0.9296472 0.759648 0.5641495 0.7326546 ASPA aspartoacylase (Canavan disease) 1618 +/- 355 1441 +/- 348 645 +/- 98 601 +/- 82 -0.004613 -0.729741 -0.743485 0.7211329 0.8423832

S2-genes differentially expressed across region.xls Page 38 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ATG16L2 ATG16 autophagy related 16-like 2 (S. cerevisiae) 298 +/- 56 283 +/- 28 167 +/- 12 132 +/- 11 -0.001216 -0.818568 -0.067976 0.6174556 0.2098369 ATIC 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase 1046 +/- 49 1113 +/- 28 796 +/- 52 837 +/- 52 -3.01E-05 0.2687251 0.581846 0.3786463 0.748156 ATP13A1 ATPase type 13A1 883 +/- 41 842 +/- 30 811 +/- 30 756 +/- 20 -0.016005 -0.43696 -0.167854 0.1642851 0.3632495 ATP2C1 ATPase, Ca++ transporting, type 2C, member 1 2029 +/- 118 1514 +/- 111 1497 +/- 112 1332 +/- 42 -0.003401 -0.009807 -0.213381 0.0042259 0.2469229 ATP5G1 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9) 4666 +/- 306 4340 +/- 232 3791 +/- 109 4046 +/- 110 -0.01373 -0.417367 0.132922 0.1087628 0.4153918 ATP6V0D1ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1 4894 +/- 273 4556 +/- 302 3696 +/- 199 4137 +/- 252 -0.005661 -0.424763 0.206387 0.1078222 0.4488251 ATXN7L3 ataxin 7-like 3 4182 +/- 141 3742 +/- 124 3753 +/- 122 3589 +/- 83 -0.010413 -0.041159 -0.297757 0.0055341 0.7757953 AURKA aurora kinase A 143 +/- 8 167 +/- 14 108 +/- 7 135 +/- 5 -0.003063 0.1749027 0.017575 0.0328976 0.0511261 AVPI1 arginine vasopressin-induced 1 540 +/- 39 555 +/- 37 400 +/- 23 385 +/- 13 -4.8E-05 0.7886688 -0.567339 0.7794425 0.5393363 AXL AXL receptor tyrosine kinase 951 +/- 51 1108 +/- 50 906 +/- 51 754 +/- 67 -0.01455 0.054106 -0.110761 0.2028006 0.0169621 AZIN1 antizyme inhibitor 1 4030 +/- 142 3939 +/- 206 3743 +/- 98 3601 +/- 141 -0.013634 -0.725887 -0.431721 0.2744238 0.1158331 B2M beta-2-microglobulin 7946 +/- 519 10622 +/- 678 6616 +/- 572 9837 +/- 646 -0.020796 0.0114966 0.005147 0.0423626 0.1051803 B3GAT1 beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) 11398 +/- 1244 9768 +/- 502 8896 +/- 432 8482 +/- 230 -0.02284 -0.266114 -0.423017 0.2414764 0.5132703 B4GALT3 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3 1509 +/- 46 1313 +/- 50 1304 +/- 35 1196 +/- 29 -0.000177 -0.016366 -0.043079 0.0059715 0.0109205 B4GALT5 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 2214 +/- 209 1568 +/- 99 1896 +/- 165 1568 +/- 81 -0.03683 -0.026376 -0.117469 0.0216119 0.1666359 BACE1 beta-site APP-cleaving enzyme 1 1499 +/- 72 1712 +/- 169 1121 +/- 69 1178 +/- 52 -0.001965 0.2870571 0.527948 0.0910948 0.5494259 BACE2 beta-site APP-cleaving enzyme 2 198 +/- 25 171 +/- 23 102 +/- 20 105 +/- 33 -0.000431 -0.452204 0.934003 0.9347589 0.4622899 BACH1 BTB and CNC homology 1, basic leucine zipper transcription factor 1 336 +/- 28 426 +/- 45 246 +/- 26 251 +/- 6 -0.001069 0.1255322 0.865043 0.1031752 0.9669212 BAD BCL2-antagonist of cell death 209 +/- 15 173 +/- 10 175 +/- 14 142 +/- 18 -0.047761 -0.079261 -0.191143 0.0926671 0.276239 BANF1 barrier to autointegration factor 1 5619 +/- 213 5422 +/- 81 4635 +/- 123 4511 +/- 76 -5.86E-06 -0.420581 -0.413525 0.5468054 0.5294386 BANP BTG3 associated nuclear protein 1318 +/- 96 1164 +/- 71 966 +/- 97 745 +/- 38 -0.00035 -0.226622 -0.074958 0.354293 0.3534149 BAT4 HLA-B associated transcript 4 923 +/- 45 833 +/- 59 747 +/- 20 734 +/- 39 -0.000314 -0.25476 -0.775151 0.2757341 0.9190475 BAX BCL2-associated X protein 831 +/- 23 877 +/- 24 728 +/- 13 807 +/- 21 -0.000268 0.188443 0.01614 0.3452937 0.1605293 BAZ1A bromodomain adjacent to zinc finger domain, 1A 183 +/- 22 197 +/- 23 162 +/- 16 140 +/- 6 -0.025496 0.6640051 -0.252279 0.232456 0.4963289 BAZ2B bromodomain adjacent to zinc finger domain, 2B 1194 +/- 122 1319 +/- 64 1005 +/- 31 954 +/- 29 -0.006499 0.3904675 -0.267764 0.2309764 0.1062429 BBX bobby sox homolog (Drosophila) 1408 +/- 90 1558 +/- 89 1131 +/- 18 1251 +/- 43 -0.00139 0.2635379 0.045523 0.1257724 0.0998663 BCAP31 B-cell receptor-associated protein 31 1029 +/- 88 1543 +/- 54 860 +/- 54 1436 +/- 92 -0.001081 0.0009896 0.001211 0.004477 0.0133562 BCAS3 breast carcinoma amplified sequence 3 899 +/- 50 839 +/- 42 779 +/- 9 787 +/- 23 -0.02314 -0.381883 0.776341 0.1978594 0.3531261 BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) 2186 +/- 49 1964 +/- 100 1929 +/- 97 1818 +/- 115 -0.042392 -0.084824 -0.480451 0.0366434 0.8290018 BCOR BCL6 co-repressor 280 +/- 31 258 +/- 25 197 +/- 8 205 +/- 19 -0.005537 -0.595716 0.71134 0.8097029 0.3866545 BCS1L BCS1-like (yeast) 1146 +/- 51 1199 +/- 38 1019 +/- 31 1085 +/- 57 -0.018167 0.4268623 0.350435 0.8144832 0.5808784 BEAN brain expressed, associated with Nedd4 306 +/- 16 345 +/- 12 264 +/- 11 295 +/- 13 -0.009825 0.0835698 0.109426 0.0355509 0.3352152 BHLHB2 basic helix-loop-helix domain containing, class B, 2 2284 +/- 239 2096 +/- 126 1184 +/- 103 1186 +/- 80 -1.48E-05 -0.50647 0.986727 0.3977209 0.9228881 BLCAP bladder cancer associated protein 6297 +/- 214 5807 +/- 369 5335 +/- 115 5220 +/- 151 -0.008169 -0.284221 -0.562069 0.1410327 0.2993708 BLM Bloom syndrome 527 +/- 29 536 +/- 16 468 +/- 19 432 +/- 17 -0.000325 0.790032 -0.184989 0.4078555 0.5534124 BLNK B-cell linker 342 +/- 38 401 +/- 43 216 +/- 10 229 +/- 18 -0.001881 0.3333053 0.546194 0.0542291 0.7412732 BLVRA biliverdin reductase A 536 +/- 46 610 +/- 47 458 +/- 22 468 +/- 22 -0.008009 0.286536 0.760519 0.3998478 0.6820022 BLVRB biliverdin reductase B (flavin reductase (NADPH)) 746 +/- 102 869 +/- 57 633 +/- 61 674 +/- 9 -0.007411 0.3219592 0.541909 0.1971174 0.4492661 BM88 BM88 antigen 2949 +/- 174 2888 +/- 181 2570 +/- 119 2243 +/- 114 -0.009952 -0.812992 -0.079298 0.7135657 0.2239279 BMP6 bone morphogenetic protein 6 200 +/- 47 173 +/- 21 143 +/- 25 157 +/- 13 -0.045627 -0.614211 0.63084 0.2322577 0.7160686 BMPR2 bone morphogenetic protein receptor, type II (serine/threonine kinase) 2903 +/- 165 2526 +/- 226 2520 +/- 106 2489 +/- 125 -0.002045 -0.209677 -0.85298 0.0531943 0.3307733 BNC2 Basonuclin 2 207 +/- 14 162 +/- 19 153 +/- 22 111 +/- 28 -0.001657 -0.086503 -0.274122 0.1780284 0.393502 BNIP3L BCL2/adenovirus E1B 19kDa interacting protein 3-like 6405 +/- 246 5854 +/- 216 5448 +/- 168 5621 +/- 257 -0.007864 -0.123451 0.590037 0.0800667 0.9906479 BOK BCL2-related ovarian killer 1816 +/- 298 2272 +/- 392 1063 +/- 118 1258 +/- 73 -0.00669 0.3784022 0.19611 0.1724041 0.1438707 BOLA2 bolA-like 2 (E. coli) 1548 +/- 67 1349 +/- 88 1261 +/- 65 1171 +/- 33 -0.000927 -0.103608 -0.255983 0.2832108 0.3709337

S2-genes differentially expressed across region.xls Page 39 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI BRAP BRCA1 associated protein 215 +/- 22 232 +/- 30 202 +/- 14 227 +/- 18 -0.049544 0.6733937 0.304641 0.9341578 0.6183887 BRCC2 BRCC2 260 +/- 26 370 +/- 35 220 +/- 11 242 +/- 10 -0.012762 0.0323446 0.172532 0.0087585 0.3163604 BRMS1 breast cancer metastasis suppressor 1 627 +/- 26 624 +/- 17 522 +/- 14 538 +/- 60 -0.00909 -0.909345 0.811949 0.935505 0.2866148 BST2 bone marrow stromal cell antigen 2 1075 +/- 131 1405 +/- 112 750 +/- 66 988 +/- 93 -0.000978 0.0849869 0.072007 0.1305267 0.2453014 BTAF1 BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolo 1066 +/- 31 1095 +/- 61 962 +/- 29 964 +/- 54 -0.001328 0.6917603 0.970487 0.194634 0.4782109 BTBD1 BTB (POZ) domain containing 1 3234 +/- 190 2975 +/- 162 2851 +/- 56 2663 +/- 63 -0.039695 -0.323669 -0.055108 0.4454523 0.2622056 BTD biotinidase 593 +/- 44 613 +/- 50 409 +/- 25 423 +/- 15 -5.51E-05 0.7736425 0.65985 0.3200071 0.6100478 BUB3 BUB3 budding uninhibited by benzimidazoles 3 homolog (yeast) 4140 +/- 127 3656 +/- 142 3469 +/- 169 3052 +/- 117 -0.000718 -0.02942 -0.074618 0.2495534 0.1460212 BVES blood vessel epicardial substance 293 +/- 14 378 +/- 50 191 +/- 15 204 +/- 17 -0.001771 0.1550702 0.562175 0.0738602 0.6090485 BZRP benzodiazapine receptor (peripheral) 565 +/- 74 798 +/- 62 374 +/- 21 504 +/- 36 -0.003355 0.0377401 0.018487 0.0214934 0.1014083 BZW1 basic leucine zipper and W2 domains 1 5216 +/- 116 4624 +/- 192 4100 +/- 120 3941 +/- 258 -0.000248 -0.028896 -0.596853 0.0047402 0.2150581 BZW2 basic leucine zipper and W2 domains 2 1355 +/- 81 1222 +/- 78 1031 +/- 49 1064 +/- 33 -0.000724 -0.26247 0.592032 0.7478192 0.2736955 C1QC complement component 1, q subcomponent, C chain 541 +/- 44 951 +/- 109 482 +/- 52 755 +/- 55 -0.028928 0.0110178 0.006202 0.0003612 0.0024066 C1QDC1 C1q domain containing 1 3407 +/- 154 3237 +/- 192 2541 +/- 134 2572 +/- 35 -0.000177 -0.506771 0.827597 0.5153574 0.7032083 C1QL1 complement component 1, q subcomponent-like 1 982 +/- 98 1014 +/- 72 913 +/- 62 825 +/- 30 -0.019822 0.8037602 -0.237101 0.7257115 0.4072478 C3 complement component 3 2677 +/- 238 3668 +/- 587 1655 +/- 154 2039 +/- 196 -0.003793 0.1642487 0.161879 0.0141031 0.1501054 C5AR1 complement component 5a receptor 1 398 +/- 25 396 +/- 23 297 +/- 14 281 +/- 16 -0.000586 -0.959126 -0.477977 0.3903895 0.4704312 CA2 carbonic anhydrase II 2992 +/- 303 2711 +/- 398 1124 +/- 92 1449 +/- 133 -0.000157 -0.586672 0.081113 0.6653623 0.0914077 CAB39 calcium binding protein 39 3226 +/- 177 2875 +/- 16 2822 +/- 86 2809 +/- 43 -0.008929 -0.104914 -0.89456 0.2203009 0.9880869 CACNA1F calcium channel, voltage-dependent, alpha 1F subunit 242 +/- 23 237 +/- 21 158 +/- 10 154 +/- 14 -0.00017 -0.862851 -0.817354 0.6575548 0.2681175 CACNA1G calcium channel, voltage-dependent, alpha 1G subunit 204 +/- 42 154 +/- 12 115 +/- 22 79 +/- 20 -0.006792 -0.294413 -0.249143 0.3128674 0.3876326 CACYBP calcyclin binding protein 6654 +/- 415 5565 +/- 323 5426 +/- 190 5114 +/- 281 -0.026934 -0.06666 -0.387609 0.1515321 0.8317044 CAMK1G calcium/calmodulin-dependent protein kinase IG 454 +/- 102 321 +/- 31 169 +/- 32 170 +/- 33 -0.008988 -0.259042 0.979103 0.1581339 0.905078 CAMK2D calcium/calmodulin-dependent protein kinase (CaM kinase) II delta 7913 +/- 785 7412 +/- 778 3163 +/- 483 2842 +/- 481 -2.02E-06 -0.659473 -0.649178 0.5348365 0.8320903 CAMKK2 calcium/calmodulin-dependent protein kinase kinase 2, beta 2835 +/- 306 2109 +/- 116 2217 +/- 96 1716 +/- 125 -0.024082 -0.065712 -0.012947 0.0168465 0.0277705 CAMSAP1 calmodulin regulated spectrin-associated protein 1 1864 +/- 55 1556 +/- 68 1653 +/- 70 1442 +/- 53 -0.000838 -0.005929 -0.039507 0.036372 0.1589263 CAP1 CAP, adenylate cyclase-associated protein 1 (yeast) 437 +/- 32 364 +/- 31 413 +/- 25 321 +/- 36 -0.018914 -0.131829 -0.069778 0.0678381 0.0342027 CAPN2 calpain 2, (m/II) large subunit 2440 +/- 204 3016 +/- 132 1931 +/- 137 2492 +/- 70 -0.000223 0.0430506 0.00757 0.0562623 0.0767471 CAPN3 calpain 3, (p94) 177 +/- 35 220 +/- 35 119 +/- 12 147 +/- 20 -0.014529 0.3989082 0.26792 0.1054587 0.0254313 CAPZB capping protein (actin filament) muscle Z-line, beta 8247 +/- 582 6857 +/- 252 6783 +/- 248 5959 +/- 245 -0.006127 -0.065542 -0.0425 0.1260651 0.0938284 CARS cysteinyl-tRNA synthetase 3163 +/- 246 2735 +/- 215 2432 +/- 118 2385 +/- 187 -0.001372 -0.220874 -0.836701 0.1018267 0.3585534 CASC3 cancer susceptibility candidate 3 2989 +/- 158 3141 +/- 57 2732 +/- 87 2752 +/- 48 -0.015715 0.3985361 0.847341 0.3257599 0.6690413 CASK calcium/calmodulin-dependent serine protein kinase (MAGUK family) 3089 +/- 307 2784 +/- 194 2506 +/- 182 2384 +/- 113 -0.004652 -0.424256 -0.583175 0.1497415 0.2021971 CAST calpastatin 990 +/- 127 1424 +/- 112 822 +/- 41 1048 +/- 19 -0.011634 0.0287645 0.00158 0.0161794 0.0076301 CAT catalase 380 +/- 36 472 +/- 86 228 +/- 22 286 +/- 38 -0.011889 0.3587229 0.226379 0.1374508 0.9282353 CBARA1 calcium binding atopy-related autoantigen 1 1698 +/- 52 1549 +/- 37 1445 +/- 72 1507 +/- 58 -0.017109 -0.04407 0.517737 0.1406721 0.701145 CBLN4 cerebellin 4 precursor 948 +/- 233 1015 +/- 167 575 +/- 35 436 +/- 67 -0.009931 0.8185865 -0.113187 0.826758 0.2379308 CCDC107 coiled-coil domain containing 107 1204 +/- 147 1523 +/- 190 1038 +/- 49 1091 +/- 74 -0.035455 0.2155086 0.56166 0.3113471 0.9487094 CCDC109Bcoiled-coil domain containing 109B 702 +/- 55 757 +/- 79 580 +/- 14 634 +/- 21 -0.016021 0.5775461 0.068799 0.2820263 0.1115963 CCDC12 coiled-coil domain containing 12 919 +/- 95 799 +/- 33 800 +/- 58 743 +/- 42 -0.035269 -0.275869 -0.455314 0.0976448 0.3226566 CCDC23 coiled-coil domain containing 23 911 +/- 80 793 +/- 55 707 +/- 57 776 +/- 17 -0.033896 -0.25815 0.291538 0.3115724 0.4504873 CCDC24 coiled-coil domain containing 24 288 +/- 17 305 +/- 25 196 +/- 18 201 +/- 21 -3.26E-05 0.5859979 0.858713 0.168644 0.3917022 CCDC4 coiled-coil domain containing 4 385 +/- 33 305 +/- 35 274 +/- 28 271 +/- 9 -0.04259 -0.121359 -0.919138 0.0865921 0.8461716 CCDC44 coiled-coil domain containing 44 792 +/- 36 669 +/- 27 578 +/- 22 598 +/- 31 -0.00364 -0.021618 0.607391 0.0106531 0.7380396 CCDC45 coiled-coil domain containing 45 450 +/- 18 407 +/- 16 364 +/- 15 322 +/- 20 -3.42E-06 -0.100746 -0.13213 0.20171 0.0710755

S2-genes differentially expressed across region.xls Page 40 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI CCDC50 coiled-coil domain containing 50 1250 +/- 119 1491 +/- 184 898 +/- 27 932 +/- 26 -0.001154 0.3021422 0.391477 0.1723793 0.3883138 CCDC72 coiled-coil domain containing 72 4579 +/- 407 4039 +/- 229 3214 +/- 212 2593 +/- 139 -0.000493 -0.281026 -0.038621 0.5045182 0.0746598 CCDC77 coiled-coil domain containing 77 406 +/- 21 425 +/- 41 352 +/- 10 375 +/- 51 -0.001461 0.6976125 0.677344 0.7048333 0.5447284 CCDC84 coiled-coil domain containing 84 574 +/- 64 492 +/- 41 476 +/- 29 359 +/- 41 -0.004905 -0.311384 -0.049202 0.5339314 0.2964875 CCDC98 coiled-coil domain containing 98 121 +/- 10 163 +/- 15 95 +/- 14 126 +/- 13 -0.036337 0.0442752 0.141438 0.0179696 0.319486 CCDC99 Coiled-coil domain containing 99 843 +/- 108 1220 +/- 192 518 +/- 27 688 +/- 24 -0.003641 0.1255292 0.001168 0.0422772 0.0247073 CCM2 cerebral cavernous malformation 2 1736 +/- 55 1623 +/- 72 1383 +/- 47 1386 +/- 25 -0.00015 -0.239711 0.952121 0.0843132 0.7609951 CCNA1 cyclin A1 165 +/- 10 276 +/- 37 154 +/- 14 177 +/- 31 -0.03939 0.0301126 0.505994 0.0100349 0.5542809 CCNDBP1 cyclin D-type binding-protein 1 1466 +/- 70 1579 +/- 106 1288 +/- 57 1562 +/- 89 -0.009416 0.3968733 0.035355 0.750233 0.1385804 CCR1 chemokine (C-C motif) receptor 1 508 +/- 66 686 +/- 92 422 +/- 42 439 +/- 49 -0.011561 0.1485346 0.79297 0.0482147 0.6272116 CCT3 chaperonin containing TCP1, subunit 3 (gamma) 2068 +/- 138 2872 +/- 102 1999 +/- 97 2727 +/- 72 -0.051148 0.0010854 0.000218 0.0758965 0.0618846 CCT4 chaperonin containing TCP1, subunit 4 (delta) 7126 +/- 125 6542 +/- 349 6285 +/- 206 6033 +/- 201 -0.003184 -0.164119 -0.403413 0.0336816 0.1625099 CD1D CD1d molecule 135 +/- 21 161 +/- 8 104 +/- 12 124 +/- 12 -0.01181 0.2834532 0.248772 0.1866177 0.3856134 CD200 CD200 molecule 10117 +/- 348 9051 +/- 463 8280 +/- 173 8089 +/- 224 -0.000309 -0.097653 -0.518914 0.0088964 0.0639654 CD2BP2 CD2 (cytoplasmic tail) binding protein 2 434 +/- 27 533 +/- 24 382 +/- 9 508 +/- 28 -0.052734 0.0224622 0.007719 0.1918141 0.0223613 CD320 CD320 molecule 549 +/- 24 584 +/- 28 467 +/- 29 468 +/- 31 -0.003082 0.3709861 0.970382 0.3795205 0.5724715 CD37 CD37 molecule 340 +/- 29 391 +/- 47 266 +/- 7 287 +/- 28 -0.016525 0.3776994 0.484042 0.0576715 0.7448742 CD48 CD48 molecule 394 +/- 43 577 +/- 75 274 +/- 24 387 +/- 42 -0.003904 0.0660649 0.055195 0.0056324 0.040375 CD52 CD52 molecule 303 +/- 58 621 +/- 52 251 +/- 51 388 +/- 37 -0.01515 0.0023325 0.057454 0.0100072 0.2205889 CD55 CD55 molecule, decay accelerating factor for complement (Cromer blood group) 367 +/- 42 394 +/- 80 265 +/- 26 278 +/- 29 -0.047118 0.7764513 0.750849 0.2936239 0.627126 CD58 CD58 molecule 179 +/- 17 277 +/- 45 140 +/- 9 195 +/- 13 -0.021073 0.0842023 0.010655 0.0174593 0.0055731 CD63 CD63 molecule 3961 +/- 565 4903 +/- 297 3173 +/- 139 3664 +/- 165 -0.011074 0.1803626 0.050996 0.198092 0.4036024 CD74 CD74 molecule, major histocompatibility complex, class II invariant chain 1810 +/- 283 3714 +/- 547 1215 +/- 171 2317 +/- 340 -0.016033 0.016153 0.027629 0.0079133 0.1049361 CD83 CD83 molecule 590 +/- 71 654 +/- 44 464 +/- 30 474 +/- 42 -0.007547 0.4713149 0.855211 0.3224844 0.5411394 CD86 CD86 molecule 127 +/- 14 208 +/- 39 86 +/- 6 132 +/- 24 -0.011942 0.0945678 0.123421 0.0061986 0.1125134 CD93 CD93 molecule 186 +/- 7 162 +/- 12 144 +/- 15 142 +/- 6 -0.001551 -0.123185 -0.920462 0.041405 0.7045664 CDC14B CDC14 cell division cycle 14 homolog B (S. cerevisiae) 236 +/- 22 203 +/- 22 176 +/- 21 160 +/- 15 -0.000214 -0.304794 -0.544328 0.4365035 0.6022839 CDC16 CDC16 cell division cycle 16 homolog (S. cerevisiae) 1071 +/- 33 1180 +/- 23 991 +/- 39 1063 +/- 17 -8.94E-05 0.0248663 0.13443 0.1310045 0.324503 CDC20 CDC20 cell division cycle 20 homolog (S. cerevisiae) 139 +/- 14 153 +/- 23 66 +/- 17 82 +/- 6 -0.00066 0.6150645 0.398175 0.287569 0.4926364 CDC25A cell division cycle 25A 235 +/- 35 330 +/- 18 224 +/- 25 264 +/- 25 -0.041807 0.0419309 0.285905 0.2069823 0.6622406 CDC26 cell division cycle 26 1490 +/- 41 1571 +/- 73 1266 +/- 44 1267 +/- 31 -0.000221 0.360711 0.978095 0.0845525 0.9835508 CDC42EP2CDC42 effector protein (Rho GTPase binding) 2 110 +/- 12 153 +/- 20 83 +/- 10 110 +/- 10 -0.033348 0.0956561 0.086805 0.0222289 0.0497078 CDC91L1 CDC91 cell division cycle 91-like 1 (S. cerevisiae) 598 +/- 28 647 +/- 38 522 +/- 14 512 +/- 19 -0.00171 0.3203868 -0.682265 0.0518592 0.8244477 CDCA7L cell division cycle associated 7-like 440 +/- 76 615 +/- 92 252 +/- 19 331 +/- 31 -0.001117 0.1753182 0.065602 0.0350888 0.0044514 CDH4 cadherin 4, type 1, R-cadherin (retinal) 468 +/- 43 438 +/- 52 352 +/- 33 387 +/- 31 -0.045426 -0.666822 0.445024 0.7600655 0.2423994 CDH5 cadherin 5, type 2, VE-cadherin (vascular epithelium) 209 +/- 8 206 +/- 15 146 +/- 9 153 +/- 21 -0.000349 -0.887945 0.768577 0.628134 0.4073582 CDK4 cyclin-dependent kinase 4 845 +/- 25 921 +/- 62 765 +/- 26 811 +/- 30 -0.022109 0.2970694 0.275314 0.0747921 0.3169219 CDK5R2 cyclin-dependent kinase 5, regulatory subunit 2 (p39) 6071 +/- 411 5283 +/- 483 4217 +/- 165 4091 +/- 295 -0.004431 -0.24351 -0.721818 0.1226054 0.8391592 CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) 1769 +/- 133 2136 +/- 160 1637 +/- 58 1549 +/- 26 -0.017152 0.1083299 -0.20917 0.0565452 0.1360662 CDKN1C Cyclin-dependent kinase inhibitor 1C (p57, Kip2) 1939 +/- 365 2891 +/- 608 950 +/- 126 1077 +/- 10 -0.004385 0.215247 0.360802 0.1337689 0.3626213 CDKN3 cyclin-dependent kinase inhibitor 3 (CDK2-associated dual specificity phosphatase) 120 +/- 8 133 +/- 6 95 +/- 3 93 +/- 5 -0.000273 0.2385591 -0.763096 0.6773918 0.8901373 CDS1 CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 2137 +/- 201 1588 +/- 146 1255 +/- 83 1183 +/- 110 -0.001244 -0.054361 -0.618529 0.0098995 0.1633426 CDYL chromodomain protein, Y-like 526 +/- 22 496 +/- 36 434 +/- 27 374 +/- 26 -0.000757 -0.499769 -0.147671 0.8702822 0.327993 CEBPG CCAAT/enhancer binding protein (C/EBP), gamma 1085 +/- 18 1141 +/- 43 915 +/- 21 949 +/- 30 -7.44E-08 0.267795 0.380616 0.3376512 0.4576828 CEP110 centrosomal protein 110kDa 305 +/- 21 344 +/- 33 256 +/- 16 276 +/- 17 -0.018392 0.3395418 0.424021 0.2373612 0.5721337

S2-genes differentially expressed across region.xls Page 41 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI CFC1 cripto, FRL-1, cryptic family 1 204 +/- 53 194 +/- 25 132 +/- 21 136 +/- 19 -0.029513 -0.871633 0.88259 0.8342035 0.9226345 CFD complement factor D (adipsin) 636 +/- 37 806 +/- 68 459 +/- 49 496 +/- 23 -0.001454 0.0607964 0.513996 0.0158211 0.7474582 CGI-38 brain specific protein 1185 +/- 160 1693 +/- 328 699 +/- 44 901 +/- 69 -0.01281 0.2043037 0.04339 0.1728503 0.1358712 CGNL1 cingulin-like 1 570 +/- 49 495 +/- 29 369 +/- 22 353 +/- 15 -1.12E-05 -0.227407 -0.552722 0.2423364 0.498816 CHCHD1 coiled-coil-helix-coiled-coil-helix domain containing 1 1511 +/- 99 1473 +/- 61 1365 +/- 29 1298 +/- 41 -0.011043 -0.753117 -0.222667 0.3291322 0.1596994 CHCHD4 coiled-coil-helix-coiled-coil-helix domain containing 4 566 +/- 39 497 +/- 32 476 +/- 27 398 +/- 11 -9.49E-05 -0.201579 -0.032704 0.2495831 0.0645877 CHFR checkpoint with forkhead and ring finger domains 1732 +/- 127 1777 +/- 97 1255 +/- 74 1243 +/- 79 -0.000117 0.7849809 -0.915752 0.646859 0.8979464 ChGn Chondroitin beta1,4 N-acetylgalactosaminyltransferase 117 +/- 12 160 +/- 11 80 +/- 9 120 +/- 16 -0.010554 0.0304215 0.074225 0.0509525 0.0348366 CHKB choline kinase beta 1172 +/- 97 1316 +/- 106 1019 +/- 71 1088 +/- 72 -0.022472 0.3394214 0.513408 0.768875 0.7856563 CHL1 cell adhesion molecule with homology to L1CAM (close homolog of L1) 5666 +/- 319 6673 +/- 332 5043 +/- 360 5085 +/- 214 -0.004389 0.0537679 0.922984 0.26906 0.9666862 CHMP2A chromatin modifying protein 2A 2413 +/- 67 2208 +/- 90 1884 +/- 39 1919 +/- 60 -0.000236 -0.099905 0.639603 0.1340368 0.6911937 CHMP4B chromatin modifying protein 4B 7492 +/- 280 6828 +/- 181 6337 +/- 187 6537 +/- 75 -0.005706 -0.079052 0.355853 0.0342813 0.2974919 CHMP6 chromatin modifying protein 6 455 +/- 12 534 +/- 45 403 +/- 23 454 +/- 21 -0.005973 0.1429622 0.140388 0.2109581 0.8072828 CHMP7 CHMP family, member 7 1152 +/- 54 1137 +/- 37 941 +/- 27 1028 +/- 35 -0.001275 -0.823906 0.084549 0.6461979 0.0676543 CHODL chondrolectin 272 +/- 39 306 +/- 35 185 +/- 15 209 +/- 24 -0.003126 0.5266179 0.429431 0.2657017 0.094144 CHRNB1 cholinergic receptor, nicotinic, beta 1 (muscle) 429 +/- 39 368 +/- 32 303 +/- 12 356 +/- 34 -0.021022 -0.255701 0.204894 0.3749951 0.3565374 CHST3 carbohydrate (chondroitin 6) sulfotransferase 3 639 +/- 84 773 +/- 125 385 +/- 5 482 +/- 26 -0.005146 0.3983593 0.019465 0.1758969 0.0445424 CHST8 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 856 +/- 116 664 +/- 49 471 +/- 61 480 +/- 37 -0.009209 -0.172908 0.900243 0.1233535 0.949405 CHURC1 churchill domain containing 1 2879 +/- 78 3384 +/- 118 2472 +/- 75 2873 +/- 90 -0.000854 0.006514 0.008507 0.0013983 0.0100486 CIB1 calcium and integrin binding 1 (calmyrin) 763 +/- 61 776 +/- 44 577 +/- 33 613 +/- 27 -0.00194 0.8647032 0.421586 0.7861799 0.8221939 CITED4 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4 176 +/- 20 190 +/- 15 131 +/- 7 133 +/- 6 -0.00089 0.6001236 0.814855 0.9260024 0.6290701 CKLF chemokine-like factor 686 +/- 32 579 +/- 45 611 +/- 22 492 +/- 40 -0.011824 -0.086706 -0.037465 0.2042547 0.0197104 CKMT1B creatine kinase, mitochondrial 1B 6947 +/- 213 5592 +/- 354 5522 +/- 246 4526 +/- 212 -0.00021 -0.010828 -0.013362 0.013891 0.1270608 CKS1B CDC28 protein kinase regulatory subunit 1B 822 +/- 24 945 +/- 35 686 +/- 28 763 +/- 14 -0.000153 0.0180419 0.041455 0.0451127 0.2475416 CLCA1 chloride channel, calcium activated, family member 1 154 +/- 63 323 +/- 100 56 +/- 17 112 +/- 21 -0.021548 0.1890092 0.064658 0.0077885 0.0043554 CLCC1 chloride channel CLIC-like 1 332 +/- 29 441 +/- 25 310 +/- 14 395 +/- 5 -0.051789 0.0179946 0.001145 0.066525 0.011344 CLDN15 claudin 15 352 +/- 48 618 +/- 92 272 +/- 21 393 +/- 56 -0.005536 0.0355177 0.099515 0.0144859 0.0386216 CLDND1 claudin domain containing 1 8178 +/- 1113 11194 +/- 1739 4771 +/- 507 6760 +/- 758 -0.008637 0.1799766 0.064248 0.0538869 0.1418948 CLIC1 chloride intracellular channel 1 450 +/- 32 468 +/- 12 341 +/- 23 402 +/- 15 -3.93E-05 0.605477 0.058328 0.7900033 0.4782277 CLK1 CDC-like kinase 1 4770 +/- 372 4554 +/- 254 4356 +/- 206 3850 +/- 108 -0.037174 -0.642794 -0.064031 0.9239291 0.1542996 CLK4 CDC-like kinase 4 2680 +/- 52 2262 +/- 87 2336 +/- 119 1847 +/- 72 -1.37E-05 -0.003237 -0.00787 0.0890823 0.0935707 CLMN calmin (calponin-like, transmembrane) 580 +/- 69 728 +/- 158 301 +/- 52 394 +/- 61 -0.008414 0.419897 0.279282 0.1286718 0.3338183 CLN5 ceroid-lipofuscinosis, neuronal 5 617 +/- 55 568 +/- 41 522 +/- 35 439 +/- 19 -0.010704 -0.49019 -0.075743 0.9144284 0.0715605 CLPTM1 cleft lip and palate associated transmembrane protein 1 3386 +/- 62 3647 +/- 150 3115 +/- 57 3343 +/- 158 -0.000817 0.155692 0.231743 0.7635097 0.4392052 CLU clusterin 3878 +/- 541 3451 +/- 377 2851 +/- 426 2733 +/- 149 -0.020096 -0.533241 -0.802516 0.8002128 0.7249083 CMTM2 CKLF-like MARVEL transmembrane domain containing 2 150 +/- 19 128 +/- 11 109 +/- 19 96 +/- 14 -0.016372 -0.347331 -0.59341 0.5361555 0.9939926 CMTM3 CKLF-like MARVEL transmembrane domain containing 3 684 +/- 73 783 +/- 21 553 +/- 20 645 +/- 18 -0.004933 0.2413487 0.007506 0.1880707 0.0672783 CMTM5 CKLF-like MARVEL transmembrane domain containing 5 2631 +/- 293 3487 +/- 483 1346 +/- 59 1861 +/- 191 -0.000853 0.1669512 0.051693 0.069623 0.0709191 CMTM7 CKLF-like MARVEL transmembrane domain containing 7 335 +/- 14 306 +/- 20 275 +/- 21 276 +/- 21 -0.016541 -0.254926 0.964587 0.0929472 0.460033 CNDP1 carnosine dipeptidase 1 (metallopeptidase M20 family) 1872 +/- 548 3446 +/- 569 974 +/- 183 2032 +/- 172 -0.014765 0.0742997 0.002279 0.0431478 0.006867 CNDP2 CNDP dipeptidase 2 (metallopeptidase M20 family) 4336 +/- 335 4565 +/- 314 3112 +/- 53 3220 +/- 84 -0.000241 0.6286596 0.310143 0.552157 0.8870322 CNFN cornifelin 249 +/- 35 281 +/- 22 219 +/- 15 214 +/- 12 -0.048069 0.4703657 -0.808291 0.8704765 0.5061143 CNP 2',3'-cyclic nucleotide 3' phosphodiesterase 8116 +/- 1232 11141 +/- 2317 4506 +/- 307 5837 +/- 379 -0.0058 0.2839787 0.025495 0.0954527 0.0080065 CNTN2 contactin 2 (axonal) 1460 +/- 297 1616 +/- 311 695 +/- 83 672 +/- 63 -0.005522 0.7247294 -0.827563 0.4145266 0.7630422 CNTN3 contactin 3 (plasmacytoma associated) 447 +/- 20 399 +/- 15 388 +/- 29 293 +/- 34 -0.003086 -0.091245 -0.064052 0.0416454 0.2389211

S2-genes differentially expressed across region.xls Page 42 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI CNTNAP5 contactin associated protein-like 5 325 +/- 36 243 +/- 23 184 +/- 13 158 +/- 9 -0.000282 -0.086368 -0.149139 0.0457127 0.2825933 COASY Coenzyme A synthase 627 +/- 42 567 +/- 29 486 +/- 23 439 +/- 20 -0.001244 -0.270332 -0.157179 0.3994476 0.5245985 COG5 component of oligomeric golgi complex 5 690 +/- 66 715 +/- 31 579 +/- 32 577 +/- 21 -0.002405 0.7426074 -0.959618 0.5414251 0.7348352 COG6 component of oligomeric golgi complex 6 1115 +/- 85 1119 +/- 39 1026 +/- 28 1056 +/- 44 -0.027176 0.9737657 0.577801 0.9369848 0.5411019 COL5A2 collagen, type V, alpha 2 370 +/- 33 376 +/- 27 313 +/- 22 284 +/- 16 -0.005388 0.8926962 -0.317904 0.7735424 0.1832831 COL8A1 Collagen, type VIII, alpha 1 97 +/- 13 150 +/- 14 69 +/- 14 113 +/- 15 -0.023159 0.0164826 0.061623 0.0556603 0.0055949 COL9A2 collagen, type IX, alpha 2 2080 +/- 276 2457 +/- 345 1452 +/- 77 1666 +/- 161 -0.018675 0.4141915 0.277935 0.3059566 0.7099132 COMMD4 COMM domain containing 4 1930 +/- 147 2000 +/- 71 1418 +/- 76 1580 +/- 67 -2.9E-05 0.6792306 0.145219 0.5970648 0.4660706 COP1 caspase-1 dominant-negative inhibitor pseudo-ICE 118 +/- 12 144 +/- 28 95 +/- 6 76 +/- 10 -0.033682 0.4210082 -0.145496 0.0687351 0.321298 COPZ2 coatomer protein complex, subunit zeta 2 410 +/- 43 502 +/- 44 289 +/- 23 302 +/- 21 -0.000483 0.1649153 0.67809 0.0992265 0.9260088 COQ5 coenzyme Q5 homolog, methyltransferase (S. cerevisiae) 581 +/- 71 553 +/- 56 486 +/- 54 495 +/- 30 -0.003981 -0.762039 0.891731 0.9596295 0.8906728 CORO1A coronin, actin binding protein, 1A 1257 +/- 114 1020 +/- 57 834 +/- 46 816 +/- 54 -0.004675 -0.102708 -0.80123 0.1541214 0.8940173 CORO1B coronin, actin binding protein, 1B 452 +/- 65 514 +/- 58 344 +/- 20 331 +/- 13 -0.013343 0.4981705 -0.610323 0.4446883 0.4003394 CORO1C coronin, actin binding protein, 1C 1945 +/- 199 1698 +/- 55 1268 +/- 64 1244 +/- 86 -0.00105 -0.27734 -0.826591 0.3309357 0.9284348 CORO2A Coronin, actin binding protein, 2A 276 +/- 74 283 +/- 50 195 +/- 23 169 +/- 14 -0.041583 0.9356185 -0.360214 0.8854581 0.5596585 COX5B cytochrome c oxidase subunit Vb 6825 +/- 788 9221 +/- 528 4736 +/- 885 7292 +/- 122 -3.18E-05 0.0331089 0.03388 0.0665571 0.0655704 COX6A1 cytochrome c oxidase subunit VIa polypeptide 1 2380 +/- 151 2589 +/- 131 2121 +/- 157 2189 +/- 96 -0.004083 0.3191634 0.721927 0.7688196 0.8338715 COX6C cytochrome c oxidase subunit VIc 7080 +/- 300 8976 +/- 497 6419 +/- 237 8142 +/- 260 -0.027156 0.0110211 0.000959 0.2042941 0.0616353 COX7A1 cytochrome c oxidase subunit VIIa polypeptide 1 (muscle) 2121 +/- 162 2252 +/- 141 1510 +/- 121 1727 +/- 103 -0.000127 0.555656 0.204574 0.282774 0.1505355 COX7A2 cytochrome c oxidase subunit VIIa polypeptide 2 (liver) 15324 +/- 717 14542 +/- 939 12731 +/- 389 12708 +/- 852 -0.000599 -0.523988 -0.981198 0.1169768 0.1868283 COX7A2L cytochrome c oxidase subunit VIIa polypeptide 2 like 868 +/- 27 853 +/- 24 800 +/- 29 748 +/- 33 -0.004685 -0.693418 -0.270303 0.6377515 0.2469298 COX7C cytochrome c oxidase subunit VIIc 10606 +/- 617 9242 +/- 390 8355 +/- 280 8437 +/- 325 -0.002098 -0.096629 0.852578 0.039567 0.8537689 CP110 CP110 protein 316 +/- 38 427 +/- 84 266 +/- 35 341 +/- 42 -0.045713 0.268209 0.204427 0.0148117 0.0252812 CPAMD8 C3 and PZP-like, alpha-2-macroglobulin domain containing 8 404 +/- 74 343 +/- 32 271 +/- 35 193 +/- 14 -0.000728 -0.471263 -0.084415 0.3925015 0.1122125 CPLX3 complexin 3 825 +/- 307 690 +/- 172 272 +/- 42 188 +/- 27 -0.005969 -0.711267 -0.127072 0.7480225 0.2834839 CPM carboxypeptidase M 510 +/- 36 719 +/- 162 320 +/- 31 381 +/- 32 -0.010773 0.2575553 0.203373 0.0179931 0.1028616 CPSF3 cleavage and polyadenylation specific factor 3, 73kDa 1455 +/- 65 1526 +/- 71 1228 +/- 39 1231 +/- 37 -0.002023 0.4757292 0.956525 0.3320076 0.8323454 CPSF4 cleavage and polyadenylation specific factor 4, 30kDa 1388 +/- 119 1359 +/- 42 1127 +/- 55 1148 +/- 45 -0.006904 -0.829127 0.778144 0.7054476 0.5413253 CRAMP1L Crm, cramped-like (Drosophila) 799 +/- 22 1083 +/- 81 759 +/- 43 853 +/- 75 -0.02985 0.0158006 0.319566 0.109389 0.8164495 CRBN cereblon 4636 +/- 161 4288 +/- 215 3986 +/- 132 3919 +/- 238 -0.000982 -0.225906 -0.812653 0.0080603 0.1939425 CREB3L1 cAMP responsive element binding protein 3-like 1 189 +/- 18 180 +/- 11 129 +/- 16 153 +/- 14 -0.007422 -0.68332 0.286968 0.2869703 0.5171331 CREB5 cAMP responsive element binding protein 5 345 +/- 71 521 +/- 109 181 +/- 16 223 +/- 14 -0.005129 0.2097603 0.080698 0.0473383 0.0667082 CREBL1 cAMP responsive element binding protein-like 1 522 +/- 42 565 +/- 23 446 +/- 18 460 +/- 29 -0.010171 0.3931707 0.70253 0.3685313 0.3114359 CREG1 cellular repressor of E1A-stimulated genes 1 1583 +/- 121 1899 +/- 179 1341 +/- 149 1470 +/- 146 -0.004148 0.1776922 0.551809 0.0098578 0.30433 CREM cAMP responsive element modulator 740 +/- 125 542 +/- 24 499 +/- 31 481 +/- 19 -0.023758 -0.175598 -0.635624 0.1193692 0.4861042 CRIM1 cysteine rich transmembrane BMP regulator 1 (chordin-like) 4775 +/- 419 3704 +/- 278 2971 +/- 172 2522 +/- 262 -0.001812 -0.063243 -0.195364 0.1499319 0.3960486 CRKL v-crk sarcoma virus CT10 oncogene homolog (avian)-like 1767 +/- 44 1717 +/- 67 1489 +/- 11 1401 +/- 15 -2.12E-05 -0.547481 -0.001865 0.9929446 0.0092549 CRR9 cisplatin resistance related protein CRR9p 2783 +/- 165 2725 +/- 158 2109 +/- 71 2119 +/- 54 -0.000669 -0.805264 0.911848 0.8654179 0.2676213 CRYAB crystallin, alpha B 11364 +/- 1007 13433 +/- 1482 8015 +/- 574 9478 +/- 561 -0.006287 0.2787117 0.101862 0.0900014 0.1000622 CRYM crystallin, mu 9716 +/- 788 8920 +/- 1069 5034 +/- 526 5665 +/- 360 -9.96E-05 -0.563728 0.349823 0.2800552 0.8508624 CSE1L CSE1 chromosome segregation 1-like (yeast) 3286 +/- 73 3432 +/- 127 2679 +/- 80 2915 +/- 76 -2.47E-06 0.350729 0.061084 0.5774952 0.2957668 CSF2RB colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) 151 +/- 7 191 +/- 15 133 +/- 12 133 +/- 8 -0.007943 0.0451243 -0.994117 0.0008691 0.7750291 CSNK2A2 casein kinase 2, alpha prime polypeptide 549 +/- 14 523 +/- 29 468 +/- 15 484 +/- 16 -0.014569 -0.460198 0.483988 0.6746479 0.6557905 CSNK2B casein kinase 2, beta polypeptide 5331 +/- 209 5162 +/- 171 4911 +/- 68 4721 +/- 101 -0.001806 -0.544323 -0.160066 0.6968934 0.8700755 CSRP1 cysteine and glycine-rich protein 1 10474 +/- 785 12051 +/- 791 7739 +/- 205 8522 +/- 429 -0.000707 0.1872883 0.153005 0.053743 0.6262066

S2-genes differentially expressed across region.xls Page 43 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI CSRP2 cysteine and glycine-rich protein 2 211 +/- 30 185 +/- 24 159 +/- 19 145 +/- 11 -0.033657 -0.531955 -0.553877 0.4985332 0.4271501 CSTB cystatin B (stefin B) 6756 +/- 454 7724 +/- 450 6112 +/- 199 7133 +/- 147 -0.030487 0.1610221 0.002746 0.2614149 0.0407972 CSTF2T cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant 319 +/- 30 389 +/- 19 279 +/- 22 321 +/- 19 -0.000598 0.0810061 0.184758 0.1481679 0.4097779 CSTF3 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa 591 +/- 22 545 +/- 19 524 +/- 22 521 +/- 21 -0.043912 -0.140529 -0.922226 0.2859229 0.7890716 CTAGE5 CTAGE family, member 5 542 +/- 50 613 +/- 67 463 +/- 22 425 +/- 40 -0.008101 0.4224351 -0.427376 0.0632479 0.8484196 CTDSP2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 2715 +/- 105 2657 +/- 150 2420 +/- 54 2153 +/- 92 -0.000546 -0.758013 -0.04211 0.7117736 0.1471537 CTGF connective tissue growth factor 756 +/- 161 908 +/- 140 271 +/- 40 368 +/- 55 -0.000694 0.4949783 0.193561 0.7186414 0.288553 CTHRC1 collagen triple helix repeat containing 1 391 +/- 48 395 +/- 20 283 +/- 50 300 +/- 42 -0.00317 0.9371471 0.806473 0.8716606 0.7262983 CTNNA1 catenin (cadherin-associated protein), alpha 1, 102kDa 1297 +/- 102 1593 +/- 116 1048 +/- 44 1115 +/- 39 -0.002255 0.0843797 0.278267 0.0385138 0.8219737 CTNND1 catenin (cadherin-associated protein), delta 1 637 +/- 49 718 +/- 21 604 +/- 44 615 +/- 22 -0.035106 0.1772398 0.829645 0.2934047 0.6295722 CTNND2 catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat prote 3584 +/- 180 3514 +/- 224 2908 +/- 77 2899 +/- 72 -0.002084 -0.811785 -0.931461 0.9720678 0.6668659 CUEDC2 CUE domain containing 2 3749 +/- 117 3595 +/- 94 3133 +/- 60 3081 +/- 20 -2.08E-05 -0.330031 -0.437773 0.0988349 0.4121574 CUL3 cullin 3 487 +/- 49 505 +/- 47 409 +/- 29 416 +/- 14 -0.003313 0.7940857 0.8386 0.4797853 0.7534171 CUTA cutA divalent cation tolerance homolog (E. coli) 2675 +/- 120 2832 +/- 174 2482 +/- 147 2291 +/- 125 -0.02037 0.4764614 -0.348924 0.7129541 0.2539292 CUTC cutC copper transporter homolog (E. coli) 578 +/- 24 592 +/- 54 494 +/- 25 497 +/- 38 -0.001325 0.8313859 0.94994 0.5048611 0.5248844 CWF19L2 CWF19-like 2, cell cycle control (S. pombe) 477 +/- 43 740 +/- 86 434 +/- 31 541 +/- 30 -0.053544 0.0270477 0.035306 0.0017024 0.0346302 CX3CR1 chemokine (C-X3-C motif) receptor 1 2269 +/- 226 2075 +/- 194 1738 +/- 136 1511 +/- 55 -0.001111 -0.52976 -0.168501 0.9434528 0.3390027 CXXC4 CXXC finger 4 336 +/- 31 432 +/- 16 266 +/- 21 359 +/- 24 -0.004324 0.0253939 0.01767 0.098273 0.053262 CYBA cytochrome b-245, alpha polypeptide 1308 +/- 102 1275 +/- 108 1067 +/- 80 888 +/- 62 -0.00236 -0.824324 -0.110376 0.3275515 0.6007482 CYBASC3 cytochrome b, ascorbate dependent 3 572 +/- 34 531 +/- 34 467 +/- 13 426 +/- 18 -0.001906 -0.40351 -0.098372 0.8280679 0.0418748 CYC1 cytochrome c-1 5184 +/- 150 4730 +/- 241 4549 +/- 119 4491 +/- 199 -0.018168 -0.14693 -0.810564 0.0092214 0.350359 CYLD Cylindromatosis (turban tumor syndrome) 1476 +/- 58 1288 +/- 84 1067 +/- 33 1026 +/- 43 -0.000225 -0.098503 -0.470961 0.1327949 0.4715388 CYLN2 cytoplasmic linker 2 1723 +/- 167 2123 +/- 197 1484 +/- 103 1612 +/- 63 -0.018909 0.1533753 0.317208 0.082689 0.3058558 CYP2U1 cytochrome P450, family 2, subfamily U, polypeptide 1 588 +/- 29 675 +/- 23 522 +/- 14 543 +/- 13 -0.000203 0.0432777 0.29925 0.0939982 0.3473348 D21S2056EDNA segment on chromosome 21 (unique) 2056 expressed sequence 941 +/- 105 1138 +/- 59 786 +/- 44 1023 +/- 84 -0.012674 0.1404877 0.04602 0.2599288 0.1698107 D4ST1 dermatan 4 sulfotransferase 1 165 +/- 15 183 +/- 6 123 +/- 12 154 +/- 8 -0.008236 0.3041757 0.071265 0.3077858 0.2574387 DAB2 disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) 692 +/- 53 525 +/- 58 501 +/- 39 504 +/- 29 -0.001653 -0.060277 0.954667 0.0111378 0.5244314 DAD1 defender against cell death 1 3547 +/- 95 3727 +/- 84 2771 +/- 49 2933 +/- 86 -2.75E-06 0.187388 0.14759 0.0493468 0.3702756 DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1) 859 +/- 104 790 +/- 88 686 +/- 53 604 +/- 48 -0.025635 -0.621655 -0.28306 0.3804186 0.0757875 DAPK3 death-associated protein kinase 3 980 +/- 115 863 +/- 89 684 +/- 82 729 +/- 47 -0.032469 -0.439015 0.64705 0.6875479 0.3427059 DAZAP2 DAZ associated protein 2 7917 +/- 569 8723 +/- 596 6161 +/- 477 6980 +/- 181 -0.000448 0.3510831 0.156523 0.117269 0.1919636 DBNDD1 dysbindin (dystrobrevin binding protein 1) domain containing 1 776 +/- 68 823 +/- 44 553 +/- 56 608 +/- 27 -0.004054 0.5761059 0.398768 0.9969725 0.2159216 DBNDD2 dysbindin (dystrobrevin binding protein 1) domain containing 2 6719 +/- 435 8157 +/- 929 5664 +/- 297 6675 +/- 313 -0.029077 0.2029886 0.044247 0.0499254 0.0076164 DBP D site of albumin promoter (albumin D-box) binding protein 129 +/- 20 148 +/- 14 98 +/- 17 89 +/- 10 -0.006967 0.4707602 -0.668048 0.2058911 0.4015884 DCHS1 dachsous 1 (Drosophila) 1010 +/- 114 1167 +/- 88 680 +/- 60 676 +/- 32 -0.000191 0.303339 -0.950341 0.2219554 0.9214016 DCLRE1B DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae) 229 +/- 11 243 +/- 15 186 +/- 10 186 +/- 8 -6.52E-05 0.4581383 -0.966724 0.5370239 0.826896 DCLRE1C DNA cross-link repair 1C (PSO2 homolog, S. cerevisiae) 235 +/- 32 211 +/- 25 150 +/- 3 165 +/- 11 -0.012651 -0.569095 0.24421 0.6780514 0.029606 DCTN2 dynactin 2 (p50) 4947 +/- 147 5280 +/- 167 4616 +/- 118 4855 +/- 82 -0.000893 0.165316 0.1329 0.4185021 0.217952 DCUN1D1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) 2306 +/- 41 1936 +/- 62 1971 +/- 52 1884 +/- 74 -0.002173 -0.00083 -0.365085 0.000397 0.2125893 DCUN1D4 DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae) 2977 +/- 334 2547 +/- 104 2316 +/- 83 2194 +/- 32 -0.031937 -0.265294 -0.214331 0.1588636 0.2847437 DDEF1 development and differentiation enhancing factor 1 1813 +/- 134 1564 +/- 105 1341 +/- 50 1340 +/- 19 -0.000433 -0.174365 -0.981809 0.0909203 0.8257003 DDHD1 DDHD domain containing 1 1480 +/- 235 2265 +/- 180 1320 +/- 221 1767 +/- 178 -0.001847 0.0253805 0.151224 0.1292407 0.5710365 DDT D-dopachrome tautomerase 2124 +/- 184 2217 +/- 150 1583 +/- 129 1578 +/- 167 -9.51E-05 0.7020827 -0.97974 0.395433 0.3868208 DDX17 DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 13896 +/- 610 13170 +/- 307 11855 +/- 464 11561 +/- 356 -0.004932 -0.321979 -0.627664 0.4803163 0.4090845 DDX18 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 1466 +/- 69 1407 +/- 49 1320 +/- 58 1244 +/- 43 -0.000321 -0.508073 -0.318355 0.7927342 0.4294238

S2-genes differentially expressed across region.xls Page 44 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 301 +/- 9 270 +/- 19 250 +/- 11 221 +/- 23 -0.002308 -0.172629 -0.313421 0.2619663 0.0979476 DDX26B DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 26B 381 +/- 10 396 +/- 28 356 +/- 9 341 +/- 13 -0.032645 0.6437637 -0.378567 0.6163309 0.9539329 DDX39 DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 637 +/- 63 757 +/- 41 507 +/- 25 534 +/- 21 -0.000558 0.1479424 0.432373 0.0660562 0.324358 DDX47 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 1524 +/- 140 1488 +/- 103 1368 +/- 52 1162 +/- 115 -0.004655 -0.841536 -0.156106 0.5780658 0.6302275 DDX48 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 1505 +/- 88 1696 +/- 45 1343 +/- 72 1561 +/- 54 -0.030789 0.0917214 0.038878 0.0217036 0.228826 DDX54 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 855 +/- 63 781 +/- 56 489 +/- 51 613 +/- 71 -0.000903 -0.397416 0.199961 0.4375784 0.4773648 DENND1C DENN/MADD domain containing 1C 279 +/- 20 236 +/- 9 241 +/- 20 176 +/- 16 -0.018819 -0.089721 -0.034211 0.1710881 0.0882096 DGAT2 diacylglycerol O-acyltransferase homolog 2 (mouse) 316 +/- 31 292 +/- 17 253 +/- 10 245 +/- 18 -0.013727 -0.521246 -0.722393 0.1535305 0.3606466 DHCR24 24-dehydrocholesterol reductase 1795 +/- 223 2736 +/- 414 1447 +/- 100 1797 +/- 163 -0.02642 0.0817219 0.110005 0.1142132 0.4341585 DHDH dihydrodiol dehydrogenase (dimeric) 173 +/- 11 172 +/- 17 134 +/- 12 116 +/- 5 -0.005216 -0.973499 -0.214215 0.5929071 0.3594928 DHPS deoxyhypusine synthase 796 +/- 30 755 +/- 26 696 +/- 34 641 +/- 23 -0.001098 -0.318859 -0.215239 0.1309789 0.0656294 DHRS1 dehydrogenase/reductase (SDR family) member 1 1266 +/- 69 1336 +/- 38 943 +/- 15 1002 +/- 24 -8.11E-06 0.4008152 0.07182 0.8469828 0.3190888 DHRS7 dehydrogenase/reductase (SDR family) member 7 1300 +/- 138 1424 +/- 161 907 +/- 67 960 +/- 73 -0.002539 0.5728443 0.604809 0.4569128 0.9039066 DHRS7B dehydrogenase/reductase (SDR family) member 7B 172 +/- 9 177 +/- 8 144 +/- 9 155 +/- 7 -0.010726 0.69642 0.387969 0.8531804 0.4989443 DHX16 DEAH (Asp-Glu-Ala-His) box polypeptide 16 670 +/- 35 550 +/- 12 568 +/- 33 500 +/- 24 -0.001543 -0.01723 -0.127297 0.0113765 0.3034065 DHX36 DEAH (Asp-Glu-Ala-His) box polypeptide 36 2428 +/- 119 3034 +/- 236 2322 +/- 107 2271 +/- 112 -0.0288 0.0537281 -0.750334 0.0091283 0.7833466 DHX37 DEAH (Asp-Glu-Ala-His) box polypeptide 37 343 +/- 17 390 +/- 25 286 +/- 22 339 +/- 28 -0.017602 0.1549653 0.170284 0.5727511 0.9543945 DHX40 DEAH (Asp-Glu-Ala-His) box polypeptide 40 1324 +/- 109 1371 +/- 9 1120 +/- 41 1114 +/- 44 -0.012507 0.6848303 -0.921147 0.4351592 0.5953142 DIABLO diablo homolog (Drosophila) 2692 +/- 59 2562 +/- 90 2300 +/- 58 2345 +/- 69 -0.001534 -0.258468 0.635741 0.0479766 0.5912605 DICER1 Dicer1, Dcr-1 homolog (Drosophila) 2501 +/- 90 2771 +/- 167 2261 +/- 38 2111 +/- 62 -0.00265 0.19297 -0.080482 0.0266581 0.1142624 DIP2B DIP2 disco-interacting protein 2 homolog B (Drosophila) 2784 +/- 338 3487 +/- 476 1923 +/- 60 2046 +/- 83 -0.003664 0.2595343 0.264269 0.0745389 0.0935278 DIRAS2 DIRAS family, GTP-binding RAS-like 2 6727 +/- 443 6285 +/- 413 5082 +/- 391 4626 +/- 149 -0.000105 -0.482539 -0.314331 0.3823621 0.5292168 DKFZp451ADKFZp451A211 protein 109 +/- 14 123 +/- 6 88 +/- 9 96 +/- 5 -0.003852 0.388696 0.481791 0.1317141 0.3447215 DKFZP564 DKFZP564O0823 protein 374 +/- 32 310 +/- 31 137 +/- 28 130 +/- 15 -2.07E-05 -0.182308 -0.842571 0.5191514 0.8937024 DKFZP779 PRO0845 1349 +/- 59 1055 +/- 62 1202 +/- 31 955 +/- 46 -0.002435 -0.006254 -0.002851 0.1175901 0.0847625 DLD dihydrolipoamide dehydrogenase 2403 +/- 100 2013 +/- 175 2063 +/- 44 1934 +/- 103 -0.003551 -0.08897 -0.295369 0.0580228 0.4884774 DLEU8 deleted in lymphocytic leukemia 8 330 +/- 44 467 +/- 42 228 +/- 18 277 +/- 20 -0.002854 0.0495718 0.100475 0.0142841 0.0960935 DLGAP4 discs, large (Drosophila) homolog-associated protein 4 5343 +/- 423 4150 +/- 339 3362 +/- 229 2932 +/- 190 -0.000264 -0.053687 -0.182775 0.0176151 0.2355429 DLST dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) 1089 +/- 65 1029 +/- 44 1007 +/- 49 935 +/- 25 -0.045492 -0.466363 -0.222515 0.2419215 0.1016999 DMAP1 DNA methyltransferase 1 associated protein 1 978 +/- 46 900 +/- 26 880 +/- 30 790 +/- 45 -0.010705 -0.174236 -0.140291 0.2209929 0.483699 DMN desmuslin 3032 +/- 226 3515 +/- 237 2383 +/- 127 2365 +/- 123 -0.001143 0.1710849 -0.921298 0.1816787 0.502669 DMTF1 cyclin D binding myb-like transcription factor 1 2324 +/- 160 2497 +/- 93 2237 +/- 55 2007 +/- 60 -0.025523 0.3743629 -0.021051 0.1431864 0.1538993 DNAH5 dynein, axonemal, heavy polypeptide 5 218 +/- 23 235 +/- 36 149 +/- 12 150 +/- 13 -0.010029 0.6957083 0.968096 0.9822735 0.8734867 DNAJA3 DnaJ (Hsp40) homolog, subfamily A, member 3 1490 +/- 50 1569 +/- 35 1346 +/- 61 1437 +/- 60 -0.019569 0.230657 0.311202 0.3766572 0.0410861 DNAJA5 DnaJ homology subfamily A member 5 145 +/- 11 219 +/- 23 120 +/- 15 134 +/- 16 -0.006805 0.0223379 0.546184 0.0218671 0.9701908 DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 3851 +/- 133 3442 +/- 136 3301 +/- 213 2897 +/- 45 -0.000198 -0.057154 -0.117919 0.2855382 0.2374256 DNAJB13 DnaJ (Hsp40) related, subfamily B, member 13 744 +/- 100 526 +/- 79 333 +/- 38 239 +/- 29 -2.18E-05 -0.119807 -0.082037 0.0456375 0.0794843 DNAJC14 DnaJ (Hsp40) homolog, subfamily C, member 14 968 +/- 18 1049 +/- 43 861 +/- 26 907 +/- 32 -0.000111 0.1261939 0.300634 0.1240299 0.9314316 DNAJC4 DnaJ (Hsp40) homolog, subfamily C, member 4 1453 +/- 70 1283 +/- 73 1081 +/- 44 1026 +/- 60 -9.32E-05 -0.123533 -0.488926 0.0211991 0.2608965 DNAJC8 DnaJ (Hsp40) homolog, subfamily C, member 8 3047 +/- 260 3518 +/- 99 2399 +/- 188 2856 +/- 98 -9.07E-06 0.1390022 0.065946 0.1667775 0.2228931 DNHD2 dynein heavy chain domain 2 285 +/- 75 175 +/- 59 78 +/- 18 100 +/- 29 -0.043756 -0.274975 0.53757 0.4125895 0.9290702 DNM2 dynamin 2 623 +/- 66 744 +/- 63 541 +/- 14 626 +/- 29 -0.040153 0.2143312 0.04064 0.1455557 0.0790072 DNMT1 DNA (cytosine-5-)-methyltransferase 1 1492 +/- 85 1567 +/- 21 1284 +/- 33 1244 +/- 71 -0.00143 0.4265879 -0.62086 0.2778813 0.5980369 DNTTIP2 deoxynucleotidyltransferase, terminal, interacting protein 2 1533 +/- 85 1584 +/- 47 1354 +/- 52 1492 +/- 56 -0.030032 0.609282 0.106363 0.7105215 0.5099277 DOCK5 dedicator of cytokinesis 5 1225 +/- 213 1548 +/- 310 535 +/- 70 703 +/- 41 -0.002714 0.4123773 0.071828 0.0751143 0.0425816

S2-genes differentially expressed across region.xls Page 45 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI DOCK9 Dedicator of cytokinesis 9 389 +/- 64 597 +/- 76 312 +/- 34 320 +/- 24 -0.02219 0.0639086 0.852778 0.1116284 0.8583269 DOLPP1 dolichyl pyrophosphate phosphatase 1 772 +/- 47 737 +/- 45 605 +/- 24 586 +/- 22 -0.003056 -0.607208 -0.583696 0.8155 0.7798358 DPF2 D4, zinc and double PHD fingers family 2 1230 +/- 66 1314 +/- 49 971 +/- 34 1002 +/- 17 -2E-05 0.3341748 0.433913 0.2052562 0.4839399 DPM2 dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit 870 +/- 18 1041 +/- 46 787 +/- 41 847 +/- 24 -0.004586 0.0119429 0.24205 0.0784903 0.3300363 DPM3 dolichyl-phosphate mannosyltransferase polypeptide 3 1718 +/- 70 1725 +/- 73 1331 +/- 29 1338 +/- 40 -2.16E-05 0.9448201 0.896672 0.6603977 0.5281242 DPP10 dipeptidyl-peptidase 10 989 +/- 85 984 +/- 123 752 +/- 50 645 +/- 13 -0.000366 -0.971908 -0.08378 0.7460663 0.1391857 DPY19L1 Dpy-19-like 1 (C. elegans) 219 +/- 36 292 +/- 34 155 +/- 20 190 +/- 14 -0.002063 0.1710724 0.192253 0.3076372 0.6063187 DPYSL2 Dihydropyrimidinase-like 2 414 +/- 28 507 +/- 33 403 +/- 15 391 +/- 41 -0.045935 0.0561399 -0.800796 0.0131823 0.4995106 DPYSL3 dihydropyrimidinase-like 3 1885 +/- 168 1922 +/- 214 1785 +/- 129 1607 +/- 189 -0.040995 0.894956 -0.460967 0.5263917 0.1380595 DPYSL5 dihydropyrimidinase-like 5 1113 +/- 109 1309 +/- 190 792 +/- 74 853 +/- 89 -0.003269 0.3973008 0.61627 0.5385025 0.98366 DRB1 developmentally regulated RNA-binding protein 1 747 +/- 41 661 +/- 33 647 +/- 19 614 +/- 19 -0.011181 -0.138338 -0.255377 0.0215398 0.0512189 DSCR2 Down syndrome critical region gene 2 3952 +/- 261 3478 +/- 155 3473 +/- 186 3068 +/- 211 -0.004602 -0.156197 -0.186055 0.3187667 0.4886926 DSCR3 Down syndrome critical region gene 3 1323 +/- 44 1391 +/- 71 1203 +/- 58 1299 +/- 61 -0.049855 0.4389988 0.28768 0.148534 0.1880045 DTX4 deltex 4 homolog (Drosophila) 1525 +/- 190 1311 +/- 103 1153 +/- 66 1016 +/- 47 -0.009416 -0.352117 -0.126487 0.5262212 0.212902 DUS1L dihydrouridine synthase 1-like (S. cerevisiae) 768 +/- 39 693 +/- 25 667 +/- 18 617 +/- 17 -0.022908 -0.139694 -0.079211 0.150679 0.1869882 DUS3L dihydrouridine synthase 3-like (S. cerevisiae) 283 +/- 16 306 +/- 11 221 +/- 15 291 +/- 20 -0.028388 0.273665 0.024862 0.3718957 0.1447019 DUSP10 dual specificity phosphatase 10 486 +/- 31 534 +/- 74 279 +/- 33 309 +/- 31 -0.001295 0.5715769 0.518575 0.162983 0.3068365 DUSP2 dual specificity phosphatase 2 190 +/- 25 154 +/- 19 120 +/- 11 97 +/- 5 -0.000336 -0.286294 -0.094397 0.2792268 0.2159902 DUSP6 dual specificity phosphatase 6 2476 +/- 370 1560 +/- 213 892 +/- 95 753 +/- 79 -0.001338 -0.064093 -0.290109 0.047101 0.4572996 DYM dymeclin 885 +/- 42 957 +/- 31 771 +/- 20 925 +/- 49 -0.025964 0.2012926 0.030225 0.5115503 0.0192984 DYNC1I2 dynein, cytoplasmic 1, intermediate chain 2 4419 +/- 229 4611 +/- 192 4024 +/- 127 3996 +/- 104 -0.006478 0.535568 -0.867919 0.2159818 0.6268196 DYNLRB2 dynein, light chain, roadblock-type 2 969 +/- 64 781 +/- 110 554 +/- 38 592 +/- 44 -0.007029 -0.175743 0.539316 0.347415 0.168933 DYNLT1 dynein, light chain, Tctex-type 1 1590 +/- 66 1613 +/- 65 1166 +/- 53 1304 +/- 33 -7.63E-06 0.804566 0.056801 0.6298356 0.3548616 E2F6 E2F transcription factor 6 309 +/- 33 341 +/- 23 267 +/- 21 274 +/- 15 -0.010235 0.4441386 0.791645 0.2302235 0.4433692 ECHDC1 enoyl Coenzyme A hydratase domain containing 1 1796 +/- 60 1599 +/- 72 1651 +/- 62 1404 +/- 53 -0.003041 -0.061427 -0.013714 0.6316257 0.1433039 EDG2 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 5091 +/- 735 6481 +/- 945 2992 +/- 289 3972 +/- 198 -0.006517 0.2741158 0.022002 0.0528127 0.027661 EEF1B2 eukaryotic translation elongation factor 1 beta 2 9038 +/- 382 8490 +/- 383 7693 +/- 269 6940 +/- 280 -0.000118 -0.335255 -0.085368 0.409393 0.2383939 EFCBP2 EF-hand calcium binding protein 2 1044 +/- 125 1270 +/- 149 793 +/- 79 1021 +/- 182 -0.002932 0.2726921 0.297385 0.909308 0.9838533 EFEMP2 EGF-containing fibulin-like extracellular matrix protein 2 739 +/- 71 813 +/- 81 569 +/- 39 613 +/- 41 -0.001685 0.5041821 0.464647 0.7450947 0.5355539 EFHA1 EF-hand domain family, member A1 1315 +/- 242 1782 +/- 116 1171 +/- 184 1429 +/- 96 -0.00604 0.1247375 0.252362 0.1382484 0.314598 EFHC1 EF-hand domain (C-terminal) containing 1 279 +/- 33 220 +/- 25 192 +/- 36 182 +/- 19 -0.025251 -0.1813 -0.806439 0.147156 0.3939764 EFHC2 EF-hand domain (C-terminal) containing 2 234 +/- 19 266 +/- 17 174 +/- 10 237 +/- 7 -0.003752 0.235678 0.000755 0.3789393 0.0642173 EFNB2 ephrin-B2 1795 +/- 111 1406 +/- 118 910 +/- 113 742 +/- 59 -4.69E-06 -0.03715 -0.223194 0.0464358 0.3631596 EFTUD2 elongation factor Tu GTP binding domain containing 2 2058 +/- 62 1919 +/- 84 1721 +/- 40 1758 +/- 52 -0.000176 -0.214433 0.593615 0.0260732 0.6172757 EGFL8 EGF-like-domain, multiple 8 530 +/- 42 549 +/- 24 458 +/- 24 437 +/- 20 -0.000779 0.7138991 -0.513479 0.7533771 0.3828939 EHBP1L1 EH domain binding protein 1-like 1 249 +/- 15 220 +/- 27 194 +/- 17 189 +/- 14 -0.009938 -0.363253 -0.83033 0.3097291 0.5929391 EIF2AK3 eukaryotic translation initiation factor 2-alpha kinase 3 394 +/- 45 400 +/- 39 300 +/- 14 292 +/- 9 -0.002919 0.9153484 -0.67177 0.8461236 0.7087189 EIF2AK4 eukaryotic translation initiation factor 2 alpha kinase 4 413 +/- 20 484 +/- 18 343 +/- 27 326 +/- 8 -0.000295 0.0263662 -0.570408 0.0087158 0.5093347 EIF2C2 Eukaryotic translation initiation factor 2C, 2 189 +/- 5 239 +/- 22 172 +/- 9 153 +/- 13 -0.003365 0.0782655 -0.278046 0.0180835 0.5226322 EIF2S2 eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa 892 +/- 48 779 +/- 24 742 +/- 37 686 +/- 37 -0.001173 -0.071512 -0.310929 0.1751001 0.1187861 EIF3S1 eukaryotic translation initiation factor 3, subunit 1 alpha, 35kDa 392 +/- 26 392 +/- 22 361 +/- 14 341 +/- 13 -0.044319 0.9876901 -0.324347 0.6518479 0.1609296 EIF3S3 eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa 3085 +/- 253 2946 +/- 180 2120 +/- 137 2247 +/- 52 -5.85E-05 -0.665212 0.417685 0.4929001 0.6263351 EIF3S7 eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa 2662 +/- 54 2791 +/- 67 2401 +/- 59 2691 +/- 31 -0.004037 0.1657818 0.002838 0.2860517 0.0059068 EIF3S8 eukaryotic translation initiation factor 3, subunit 8, 110kDa 3667 +/- 317 3087 +/- 171 3009 +/- 145 2951 +/- 106 -0.035598 -0.147343 -0.753654 0.1408421 0.9077313 EIF4EBP1 eukaryotic translation initiation factor 4E binding protein 1 362 +/- 23 361 +/- 37 292 +/- 13 256 +/- 28 -0.001076 -0.977605 -0.294307 0.7704956 0.6527003

S2-genes differentially expressed across region.xls Page 46 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI EIF5 eukaryotic translation initiation factor 5 8827 +/- 251 9331 +/- 262 7941 +/- 139 8250 +/- 152 -0.000234 0.1949471 0.169831 0.1857902 0.2000823 ELMO2 engulfment and cell motility 2 1952 +/- 114 1744 +/- 64 1412 +/- 137 1391 +/- 139 -0.001477 -0.150189 -0.914956 0.1107665 0.6343618 ELOVL1 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 1 1213 +/- 157 1604 +/- 287 748 +/- 28 884 +/- 79 -0.006513 0.2668783 0.164108 0.0513098 0.1862667 ELTD1 EGF, latrophilin and seven transmembrane domain containing 1 216 +/- 15 319 +/- 20 190 +/- 19 228 +/- 17 -0.026701 0.0022995 0.16525 0.0005254 0.2394358 EMG1 EMG1 nucleolar protein homolog (S. cerevisiae) 1710 +/- 78 1487 +/- 91 1238 +/- 49 1195 +/- 60 -0.000154 -0.094425 -0.593953 0.0175036 0.2306062 EMP3 epithelial membrane protein 3 385 +/- 23 473 +/- 25 311 +/- 21 374 +/- 24 -0.001739 0.0295194 0.081156 0.0951945 0.7970266 ENDOD1 endonuclease domain containing 1 204 +/- 20 244 +/- 25 170 +/- 13 174 +/- 6 -0.01548 0.2535616 0.753639 0.2457314 0.9603205 ENO1 enolase 1, (alpha) 7846 +/- 333 9198 +/- 336 6480 +/- 135 7880 +/- 253 -0.000391 0.0169672 0.00252 0.0783902 0.0568711 ENPP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) 3043 +/- 553 3566 +/- 683 1105 +/- 243 1628 +/- 295 -0.000988 0.5654331 0.207101 0.1712243 0.3085393 ENPP6 ectonucleotide pyrophosphatase/phosphodiesterase 6 311 +/- 73 377 +/- 55 171 +/- 24 208 +/- 13 -0.001622 0.4908494 0.227004 0.7249054 0.7951349 ENY2 enhancer of yellow 2 homolog (Drosophila) 1695 +/- 57 1761 +/- 66 1537 +/- 41 1584 +/- 40 -0.003072 0.4696526 0.440732 0.810821 0.9320109 EPAS1 endothelial PAS domain protein 1 3071 +/- 166 2921 +/- 93 2305 +/- 183 2256 +/- 84 -3.57E-06 -0.452943 -0.813512 0.3579985 0.5246164 EPB41L2 erythrocyte membrane protein band 4.1-like 2 5330 +/- 488 6169 +/- 576 3765 +/- 111 3975 +/- 44 -0.000887 0.2930551 0.125517 0.1425261 0.1745448 EPB41L4A erythrocyte membrane protein band 4.1 like 4A 703 +/- 69 644 +/- 118 350 +/- 37 298 +/- 18 -0.001169 -0.673735 -0.2491 0.5566313 0.5821436 EPC2 enhancer of polycomb homolog 2 (Drosophila) 1863 +/- 112 1971 +/- 30 1637 +/- 86 1711 +/- 73 -0.012254 0.3905076 0.525919 0.2103227 0.6519041 EPHA3 EPH receptor A3 372 +/- 55 421 +/- 110 240 +/- 49 185 +/- 18 -0.005647 0.7000492 -0.333694 0.7532884 0.53131 EPM2A epilepsy, progressive myoclonus type 2A, Lafora disease (laforin) 318 +/- 20 349 +/- 36 278 +/- 19 264 +/- 51 -0.012415 0.47148 -0.804046 0.8968049 0.3139062 EPS8 epidermal growth factor receptor pathway substrate 8 1375 +/- 55 1537 +/- 28 1326 +/- 75 1257 +/- 47 -0.015856 0.0333534 -0.451632 0.0403725 0.1494764 ERCC1 excision repair cross-complementing rodent repair deficiency, complementation group 1 (i 682 +/- 32 831 +/- 69 597 +/- 41 631 +/- 29 -0.015277 0.0885035 0.525301 0.0875 0.9902521 ERGIC3 ERGIC and golgi 3 2303 +/- 107 2433 +/- 73 2054 +/- 51 2319 +/- 42 -0.015545 0.3425289 0.003142 0.8177734 0.1119695 ERICH1 glutamate-rich 1 931 +/- 135 1265 +/- 111 765 +/- 145 1023 +/- 52 -0.0202 0.086356 0.142185 0.1052947 0.309919 ERO1LB ERO1-like beta (S. cerevisiae) 947 +/- 63 1073 +/- 83 814 +/- 79 937 +/- 65 -0.000811 0.2579165 0.261626 0.7228308 0.4841156 ERRFI1 ERBB receptor feedback inhibitor 1 1173 +/- 57 1147 +/- 41 1073 +/- 44 978 +/- 42 -0.038692 -0.717351 -0.152492 0.8919617 0.123803 ESRRG estrogen-related receptor gamma 778 +/- 68 735 +/- 84 490 +/- 33 480 +/- 19 -0.000272 -0.699539 -0.799959 0.3359832 0.8692106 ETFB electron-transfer-flavoprotein, beta polypeptide 2760 +/- 279 2855 +/- 163 1875 +/- 76 1935 +/- 94 -0.000253 0.775005 0.636571 0.799702 0.8054761 ETFDH electron-transferring-flavoprotein dehydrogenase 382 +/- 17 463 +/- 16 356 +/- 16 395 +/- 22 -0.011825 0.0066056 0.190975 0.1096217 0.4593126 ETV1 ets variant gene 1 702 +/- 128 593 +/- 43 429 +/- 50 390 +/- 33 -0.001468 -0.446172 -0.528891 0.3821082 0.3714505 EXOC2 exocyst complex component 2 1154 +/- 52 1250 +/- 52 1015 +/- 35 1145 +/- 65 -0.000258 0.223044 0.127503 0.7022921 0.7702248 EXOC4 exocyst complex component 4 1061 +/- 58 1164 +/- 55 820 +/- 38 927 +/- 36 -0.000573 0.2207805 0.070249 0.3513183 0.3638676 EXOSC10 exosome component 10 957 +/- 62 1170 +/- 42 800 +/- 35 895 +/- 36 -0.000661 0.0202287 0.092599 0.0296892 0.1904323 EXOSC4 exosome component 4 371 +/- 25 410 +/- 33 338 +/- 9 340 +/- 28 -0.031187 0.3674989 0.964598 0.0795553 0.8005771 EXOSC5 exosome component 5 648 +/- 33 544 +/- 16 532 +/- 31 483 +/- 14 -0.008 -0.025109 -0.20146 0.0251747 0.4908932 EXT2 exostoses (multiple) 2 970 +/- 52 841 +/- 25 802 +/- 22 759 +/- 47 -0.003722 -0.058591 -0.431526 0.3594458 0.7883677 EXTL3 exostoses (multiple)-like 3 1197 +/- 60 1026 +/- 64 936 +/- 42 954 +/- 43 -0.0339 -0.080422 0.769533 0.1122746 0.5792303 F2RL1 coagulation factor II (thrombin) receptor-like 1 106 +/- 24 135 +/- 20 79 +/- 12 92 +/- 20 -0.045948 0.3883311 0.615583 0.2548334 0.8407825 FA2H fatty acid 2-hydroxylase 1088 +/- 162 1680 +/- 349 622 +/- 81 901 +/- 73 -0.012014 0.1668608 0.030927 0.0274475 0.011596 FANCC Fanconi anemia, complementation group C 492 +/- 43 461 +/- 26 405 +/- 25 393 +/- 26 -0.008609 -0.550951 -0.756556 0.4950782 0.253809 FARP1 FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) 387 +/- 68 1140 +/- 179 336 +/- 59 910 +/- 114 -0.005662 0.0066696 0.004169 0.1398067 0.0912823 FARSLA phenylalanine-tRNA synthetase-like, alpha subunit 925 +/- 38 978 +/- 40 762 +/- 42 831 +/- 36 -0.000165 0.3620869 0.244758 0.8171697 0.6987873 FASTK Fas-activated serine/threonine kinase 625 +/- 37 797 +/- 40 549 +/- 14 706 +/- 35 -0.014604 0.010554 0.0082 0.048923 0.0043773 FAU Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derive 11577 +/- 613 11037 +/- 270 10727 +/- 416 10809 +/- 332 -0.030234 -0.446954 0.881039 0.4328387 0.9805711 FBL fibrillarin 2698 +/- 192 2717 +/- 152 2118 +/- 63 1971 +/- 56 -0.000539 0.9414396 -0.117263 0.8262261 0.2396561 FBXL15 F-box and leucine-rich repeat protein 15 628 +/- 23 580 +/- 19 529 +/- 24 562 +/- 22 -0.047834 -0.142194 0.337817 0.0947749 0.6588446 FBXL20 F-box and leucine-rich repeat protein 20 390 +/- 20 371 +/- 24 331 +/- 14 297 +/- 26 -0.003937 -0.56614 -0.304651 0.8984593 0.4724749 FBXL7 F-box and leucine-rich repeat protein 7 683 +/- 62 618 +/- 28 534 +/- 37 503 +/- 13 -0.000802 -0.374871 -0.457287 0.6892061 0.4546082

S2-genes differentially expressed across region.xls Page 47 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI FBXW7 F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila) 10339 +/- 647 9502 +/- 444 7207 +/- 268 6594 +/- 413 -6.67E-05 -0.314329 -0.252229 0.2752317 0.7455432 FCER1G Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide 283 +/- 30 393 +/- 40 195 +/- 12 277 +/- 15 -0.002161 0.0544574 0.002516 0.0320568 0.0074183 FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) 228 +/- 21 288 +/- 27 185 +/- 20 221 +/- 14 -0.020592 0.1104118 0.177116 0.0384468 0.1607987 FCGR3B Fc fragment of IgG, low affinity IIIb, receptor (CD16b) 158 +/- 14 222 +/- 21 125 +/- 11 167 +/- 20 -0.001601 0.0307221 0.112317 0.0083523 0.0039974 FCGRT Fc fragment of IgG, receptor, transporter, alpha 1436 +/- 82 1462 +/- 100 1118 +/- 52 1110 +/- 100 -6.95E-07 0.842287 -0.942242 0.8073778 0.3493553 FCHSD2 FCH and double SH3 domains 2 602 +/- 50 541 +/- 45 432 +/- 23 355 +/- 19 -0.000261 -0.3862 -0.031367 0.7664457 0.1745995 FCN3 ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen) 773 +/- 82 612 +/- 38 616 +/- 53 473 +/- 26 -0.007798 -0.119355 -0.044076 0.2732374 0.2516669 FEZ1 fasciculation and elongation protein zeta 1 (zygin I) 8114 +/- 604 9755 +/- 837 6969 +/- 218 8397 +/- 232 -0.032367 0.1460565 0.001635 0.0511895 0.0020014 FGFRL1 fibroblast growth factor receptor-like 1 394 +/- 27 446 +/- 32 345 +/- 19 370 +/- 11 -0.003687 0.2475898 0.28718 0.3559833 0.1768308 FHOD1 formin homology 2 domain containing 1 217 +/- 23 209 +/- 14 174 +/- 6 170 +/- 11 -0.010597 -0.748054 -0.757478 0.4553819 0.8876134 FHOD3 formin homology 2 domain containing 3 907 +/- 38 718 +/- 69 579 +/- 57 434 +/- 53 -1.2E-05 -0.043636 -0.096171 0.0103365 0.3282478 FIBP fibroblast growth factor (acidic) intracellular binding protein 1480 +/- 33 1419 +/- 89 1251 +/- 30 1310 +/- 73 -0.003098 -0.537754 0.486959 0.2505944 0.9064475 FIGN Fidgetin 193 +/- 22 240 +/- 26 159 +/- 11 150 +/- 19 -0.00165 0.1886099 -0.717939 0.0824478 0.700346 FIS1 fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) 3880 +/- 232 4094 +/- 258 2895 +/- 34 3141 +/- 186 -0.002259 0.5502331 0.257717 0.5529002 0.471051 FKBP1A FK506 binding protein 1A, 12kDa 97 +/- 8 145 +/- 24 74 +/- 11 99 +/- 19 -0.010683 0.0996453 0.287728 0.0226258 0.0704455 FKBP2 FK506 binding protein 2, 13kDa 4114 +/- 176 3978 +/- 228 3006 +/- 59 3192 +/- 134 -0.000312 -0.647115 0.253838 0.289261 0.5309577 FLAD1 FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) 480 +/- 17 507 +/- 18 423 +/- 22 406 +/- 19 -0.007278 0.2981438 -0.573008 0.0756129 0.7571344 FLII flightless I homolog (Drosophila) 1073 +/- 71 1217 +/- 48 871 +/- 23 1050 +/- 35 -0.002118 0.1284841 0.003355 0.1624965 0.0318487 FLJ10292 mago-nashi homolog 1261 +/- 70 1368 +/- 139 1084 +/- 60 1073 +/- 111 -0.007505 0.5131335 -0.933414 0.1717963 0.2933406 FLJ20160 FLJ20160 protein 2368 +/- 78 2623 +/- 149 1983 +/- 98 2097 +/- 64 -0.001853 0.1707144 0.357235 0.027552 0.3568701 FLJ22624 FLJ22624 protein 104 +/- 8 113 +/- 10 91 +/- 5 95 +/- 11 -0.021867 0.4694018 0.791768 0.6780549 0.6409617 FLJ25084 Skin ASpartic Protease 244 +/- 31 304 +/- 25 191 +/- 22 211 +/- 10 -0.00665 0.1606849 0.41894 0.0755458 0.2275585 FLJ25530 hepatocyte cell adhesion molecule 4362 +/- 359 5227 +/- 460 3822 +/- 130 4496 +/- 91 -0.014625 0.171006 0.002411 0.064663 0.003096 FLJ44968 FLJ44968 protein 1611 +/- 160 1591 +/- 166 1194 +/- 70 1303 +/- 48 -0.01069 -0.93357 0.23285 0.9789572 0.4866205 FLNC filamin C, gamma (actin binding protein 280) 423 +/- 64 485 +/- 58 196 +/- 21 278 +/- 15 -0.000385 0.4850993 0.012306 0.4644705 0.0496698 FLRT2 fibronectin leucine rich transmembrane protein 2 402 +/- 68 350 +/- 52 276 +/- 38 272 +/- 34 -0.024393 -0.557496 -0.941913 0.2671411 0.7750184 FLT3 fms-related tyrosine kinase 3 200 +/- 23 262 +/- 27 110 +/- 9 165 +/- 6 -0.000328 0.1216677 0.000867 0.1610886 0.0425169 FMNL3 formin-like 3 460 +/- 48 539 +/- 26 364 +/- 20 416 +/- 26 -0.005429 0.1812658 0.156208 0.1578796 0.2393277 FN1 fibronectin 1 1193 +/- 123 1012 +/- 110 905 +/- 96 749 +/- 48 -0.001069 -0.298974 -0.189998 0.0287898 0.0711712 FNBP1 Formin binding protein 1 2180 +/- 284 2827 +/- 398 1337 +/- 59 1373 +/- 54 -0.001667 0.2188058 0.666709 0.0506931 0.2454356 FNBP1L formin binding protein 1-like 1518 +/- 234 1397 +/- 114 1153 +/- 107 925 +/- 84 -0.013429 -0.655786 -0.127988 0.476082 0.251493 FNDC4 fibronectin type III domain containing 4 1758 +/- 59 1586 +/- 117 1365 +/- 57 1386 +/- 52 -0.001788 -0.232663 0.7921 0.040767 0.7491224 FOLH1 folate hydrolase (prostate-specific membrane antigen) 1 917 +/- 152 1069 +/- 251 549 +/- 87 598 +/- 99 -0.023544 0.6166617 0.717415 0.2241539 0.684578 FOXJ2 forkhead box J2 2189 +/- 117 1920 +/- 77 1475 +/- 92 1318 +/- 68 -4.45E-05 -0.089255 -0.204781 0.1117535 0.361873 FOXO3A Forkhead box O3A 331 +/- 35 512 +/- 68 283 +/- 27 375 +/- 22 -0.005025 0.0486744 0.026861 0.0574577 0.0218273 FOXP1 forkhead box P1 285 +/- 29 214 +/- 23 213 +/- 24 144 +/- 16 -0.006384 -0.086046 -0.043082 0.7429002 0.2205373 FOXRED1 FAD-dependent oxidoreductase domain containing 1 1473 +/- 29 1404 +/- 61 1245 +/- 56 1342 +/- 70 -0.02516 -0.334044 0.304842 0.1366828 0.7227087 FRAS1 Fraser syndrome 1 977 +/- 53 821 +/- 113 575 +/- 71 461 +/- 23 -6.08E-05 -0.252111 -0.177067 0.1438506 0.5209527 FRMD4A FERM domain containing 4A 926 +/- 37 796 +/- 36 829 +/- 20 663 +/- 39 -0.002359 -0.030713 -0.008665 0.1317086 0.0397146 FRYL furry homolog-like (Drosophila) 557 +/- 54 763 +/- 84 509 +/- 27 455 +/- 20 -0.037007 0.0703112 -0.140128 0.0077 0.4955705 FSD1 fibronectin type III and SPRY domain containing 1 920 +/- 35 816 +/- 42 802 +/- 30 741 +/- 32 -0.026155 -0.087406 -0.206386 0.0074091 0.2822247 FSTL1 follistatin-like 1 1444 +/- 104 1350 +/- 62 956 +/- 42 1093 +/- 20 -0.000477 -0.458445 0.021569 0.493828 0.1436632 FTL ferritin, light polypeptide 16238 +/- 1033 18500 +/- 704 14150 +/- 408 17258 +/- 618 -0.026136 0.1045271 0.00385 0.1129855 0.1026607 FTS fused toes homolog (mouse) 5169 +/- 308 4766 +/- 212 3873 +/- 239 4259 +/- 174 -0.014268 -0.309813 0.225314 0.3223975 0.3513101 FTSJ1 FtsJ homolog 1 (E. coli) 785 +/- 66 718 +/- 44 602 +/- 26 589 +/- 38 -0.003276 -0.41955 -0.785941 0.5435209 0.6997935

S2-genes differentially expressed across region.xls Page 48 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI FTSJ2 FtsJ homolog 2 (E. coli) 498 +/- 18 476 +/- 31 406 +/- 8 463 +/- 17 -0.012851 -0.548167 0.02704 0.1548927 0.031354 FTSJ3 FtsJ homolog 3 (E. coli) 505 +/- 43 529 +/- 18 404 +/- 14 474 +/- 29 -0.018588 0.6174046 0.076046 0.3370954 0.2169031 FZD4 frizzled homolog 4 (Drosophila) 592 +/- 53 458 +/- 30 398 +/- 15 384 +/- 24 -0.007693 -0.059875 -0.621091 0.0270553 0.970464 FZD6 frizzled homolog 6 (Drosophila) 299 +/- 32 257 +/- 11 211 +/- 22 207 +/- 19 -0.005079 -0.254938 -0.901009 0.4751081 0.423233 GAB2 GRB2-associated binding protein 2 1847 +/- 344 2157 +/- 306 982 +/- 34 1092 +/- 59 -0.001689 0.5161619 0.155237 0.2224056 0.0012169 GABARAP GABA(A) receptor-associated protein 11702 +/- 341 11960 +/- 190 10752 +/- 157 11400 +/- 130 -0.00177 0.5282196 0.011299 0.5584819 0.0638276 GABRA1 gamma-aminobutyric acid (GABA) A receptor, alpha 1 2907 +/- 336 3256 +/- 506 2181 +/- 178 2201 +/- 74 -0.003368 0.5803607 0.920507 0.9151562 0.7572503 GADD45B growth arrest and DNA-damage-inducible, beta 730 +/- 33 660 +/- 48 590 +/- 40 509 +/- 21 -0.00445 -0.260921 -0.110977 0.7345771 0.2523531 GAL3ST1 galactose-3-O-sulfotransferase 1 482 +/- 85 597 +/- 117 232 +/- 22 269 +/- 14 -0.003121 0.4435998 0.185218 0.1979505 0.4341002 GALNT1 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (G 1487 +/- 76 1307 +/- 95 1286 +/- 57 1262 +/- 72 -0.043484 -0.171201 -0.803101 0.1872101 0.5941416 GALNT12 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 ( 638 +/- 84 773 +/- 57 529 +/- 34 592 +/- 21 -0.014652 0.2167411 0.152624 0.2460076 0.3731398 GALNT4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (G 171 +/- 20 168 +/- 14 132 +/- 15 111 +/- 11 -0.000411 -0.886065 -0.299838 0.7395903 0.1659209 GALNT6 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (G 309 +/- 90 416 +/- 84 144 +/- 13 201 +/- 31 -0.007189 0.406547 0.145237 0.262658 0.0822429 GALNT7 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (G 507 +/- 38 593 +/- 37 358 +/- 16 368 +/- 28 -0.000144 0.1347164 0.751859 0.3556087 0.7982724 GALNTL2 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 448 +/- 45 296 +/- 38 272 +/- 18 235 +/- 32 -0.010103 -0.026776 -0.343099 0.0897514 0.3666745 GANC glucosidase, alpha; neutral C 451 +/- 60 883 +/- 120 322 +/- 16 530 +/- 33 -0.013584 0.0135516 0.001369 0.0051561 0.0059706 GAP43 growth associated protein 43 3187 +/- 286 3188 +/- 320 2477 +/- 133 2184 +/- 65 -0.001472 0.9973364 -0.086847 0.7035552 0.267515 GARNL1 GTPase activating Rap/RanGAP domain-like 1 683 +/- 64 626 +/- 46 579 +/- 30 462 +/- 37 -0.012651 -0.48589 -0.040099 0.9233998 0.0687345 GARS glycyl-tRNA synthetase 2928 +/- 135 2668 +/- 137 2369 +/- 60 2385 +/- 60 -0.002177 -0.207385 0.855197 0.0731511 0.8854352 GAS1 growth arrest-specific 1 1332 +/- 48 1196 +/- 95 1232 +/- 73 1064 +/- 38 -0.026022 -0.236095 -0.07768 0.9419286 0.3216515 GAS7 growth arrest-specific 7 9122 +/- 492 10812 +/- 463 7341 +/- 461 8180 +/- 533 -0.000193 0.0313393 0.265978 0.1668645 0.1972682 GBA glucosidase, beta; acid (includes glucosylceramidase) 340 +/- 28 340 +/- 24 273 +/- 19 283 +/- 17 -0.001428 0.9846466 0.704739 0.472304 0.3603423 GCDH glutaryl-Coenzyme A dehydrogenase 441 +/- 27 371 +/- 17 418 +/- 24 317 +/- 19 -0.043161 -0.058952 -0.009162 0.0303135 0.0002663 GCN1L1 GCN1 general control of amino-acid synthesis 1-like 1 (yeast) 678 +/- 63 753 +/- 36 547 +/- 13 570 +/- 22 -0.002305 0.3249385 0.398553 0.235447 0.2281114 Gcom1 GRINL1A combined protein 380 +/- 75 485 +/- 91 188 +/- 12 195 +/- 15 -0.001506 0.3935844 0.717825 0.4131573 0.7146714 GDAP2 ganglioside induced differentiation associated protein 2 573 +/- 31 663 +/- 36 534 +/- 33 563 +/- 34 -0.016399 0.08683 0.559845 0.1251724 0.5865968 GDI2 GDP dissociation inhibitor 2 5306 +/- 108 5016 +/- 127 4579 +/- 86 4402 +/- 99 -0.000136 -0.113474 -0.212161 0.232678 0.6120108 GDPD3 glycerophosphodiester phosphodiesterase domain containing 3 119 +/- 19 128 +/- 13 89 +/- 16 101 +/- 11 -0.040643 0.715043 0.556802 0.3444737 0.856881 GFRA2 GDNF family receptor alpha 2 784 +/- 107 694 +/- 41 624 +/- 53 523 +/- 42 -0.044649 -0.463273 -0.1683 0.3268422 0.0943996 GGA3 golgi associated, gamma adaptin ear containing, ARF binding protein 3 2318 +/- 194 2668 +/- 92 2042 +/- 88 2005 +/- 68 -0.00245 0.1463827 -0.749114 0.4001113 0.983511 GGTLA4 gamma-glutamyltransferase-like activity 4 155 +/- 2 188 +/- 13 139 +/- 10 162 +/- 8 -0.043682 0.0487262 0.12909 0.0644891 0.1799802 GIT2 G protein-coupled receptor kinase interactor 2 2774 +/- 76 3131 +/- 101 2423 +/- 103 2775 +/- 131 -0.004492 0.0196676 0.067079 0.1746952 0.0318407 GJA12 gap junction protein, alpha 12, 47kDa 683 +/- 86 921 +/- 117 493 +/- 44 660 +/- 35 -0.026775 0.1357396 0.015548 0.0902397 0.1246776 GJA7 gap junction protein, alpha 7, 45kDa (connexin 45) 242 +/- 25 209 +/- 20 154 +/- 14 145 +/- 13 -0.002071 -0.322531 -0.635095 0.4175594 0.6979369 GLA galactosidase, alpha 400 +/- 19 475 +/- 12 357 +/- 11 391 +/- 12 -0.005319 0.0101419 0.061628 0.0135964 0.3486946 GLDC glycine dehydrogenase (decarboxylating) 661 +/- 136 491 +/- 38 332 +/- 12 295 +/- 16 -0.008112 -0.273915 -0.107303 0.2292532 0.083932 GLT25D2 glycosyltransferase 25 domain containing 2 714 +/- 57 732 +/- 52 438 +/- 21 422 +/- 28 -3.91E-06 0.8198134 -0.659207 0.7293567 0.9968209 GLTP glycolipid transfer protein 651 +/- 53 840 +/- 92 531 +/- 50 675 +/- 51 -0.004488 0.1131156 0.076299 0.0379876 0.0466051 GMFG glia maturation factor, gamma 412 +/- 31 565 +/- 21 316 +/- 21 420 +/- 14 -1.16E-05 0.0027447 0.002888 0.0042369 0.048963 GMPPA GDP-mannose pyrophosphorylase A 538 +/- 19 566 +/- 20 479 +/- 14 495 +/- 22 -0.00092 0.3286272 0.562579 0.8454384 0.5011415 GMPS guanine monphosphate synthetase 570 +/- 45 619 +/- 27 438 +/- 20 530 +/- 21 -0.001525 0.3776871 0.011127 0.4369728 0.0406228 GNAI1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 3885 +/- 382 4376 +/- 455 2314 +/- 243 2600 +/- 342 -0.001308 0.4289106 0.516613 0.277299 0.6153514 GNAI3 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 2053 +/- 166 2183 +/- 82 1828 +/- 79 1864 +/- 49 -0.014175 0.5051208 0.711206 0.735016 0.6806788 GNG12 guanine nucleotide binding protein (G protein), gamma 12 2154 +/- 146 2439 +/- 165 1842 +/- 112 1621 +/- 52 -0.001139 0.2243651 -0.116575 0.1638139 0.2867848 GNG5 guanine nucleotide binding protein (G protein), gamma 5 1170 +/- 71 1069 +/- 100 919 +/- 44 998 +/- 61 -0.034156 -0.434271 0.329067 0.1440744 0.819154

S2-genes differentially expressed across region.xls Page 49 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI GOLGA7 golgi autoantigen, golgin subfamily a, 7 9156 +/- 727 11245 +/- 1212 6208 +/- 390 7268 +/- 419 -0.002789 0.1770344 0.098229 0.0400406 0.1986683 GOLGB1 golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1 1330 +/- 86 1392 +/- 33 1182 +/- 53 1174 +/- 30 -0.00163 0.5238144 -0.902338 0.5692584 0.783573 GOT2 glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) 5041 +/- 112 4879 +/- 319 3831 +/- 214 3914 +/- 71 -1.26E-05 -0.646713 0.725853 0.2427372 0.9803293 GPAA1 glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) 1271 +/- 55 1395 +/- 75 1093 +/- 43 1152 +/- 77 -0.007848 0.2139451 0.52516 0.196772 0.9134345 GPAM glycerol-3-phosphate acyltransferase, mitochondrial 1282 +/- 69 1560 +/- 140 1174 +/- 80 1164 +/- 58 -0.007966 0.1168151 -0.924631 0.1758006 0.8861056 GPATC3 G patch domain containing 3 320 +/- 15 340 +/- 17 286 +/- 13 290 +/- 20 -0.011772 0.4061917 0.87391 0.2922303 0.9308755 GPBP1 GC-rich promoter binding protein 1 3119 +/- 38 3284 +/- 79 2948 +/- 59 2947 +/- 102 -0.008832 0.1007156 -0.997791 0.1681871 0.276958 GPD1L glycerol-3-phosphate dehydrogenase 1-like 2350 +/- 255 2199 +/- 106 1785 +/- 106 1855 +/- 97 -0.009423 -0.601597 0.636576 0.3477899 0.780757 GPI glucose phosphate isomerase 6760 +/- 187 7193 +/- 293 6165 +/- 134 6432 +/- 171 -0.004869 0.2453242 0.253767 0.6267276 0.1627335 GPR108 G protein-coupled receptor 108 588 +/- 34 683 +/- 48 453 +/- 18 551 +/- 40 -0.002268 0.1420679 0.071056 0.1203747 0.4435396 GPR123 G protein-coupled receptor 123 901 +/- 107 822 +/- 84 415 +/- 43 466 +/- 54 -2.03E-05 -0.574612 0.483517 0.2096511 0.4761395 GPR161 G protein-coupled receptor 161 343 +/- 64 227 +/- 15 188 +/- 10 151 +/- 20 -0.014845 -0.130879 -0.146649 0.1539986 0.3639722 GPR17 G protein-coupled receptor 17 224 +/- 28 272 +/- 34 167 +/- 11 176 +/- 16 -0.011206 0.3069854 0.652405 0.1464185 0.6509733 GPR177 G protein-coupled receptor 177 880 +/- 63 834 +/- 57 708 +/- 32 751 +/- 48 -0.008557 -0.607869 0.480375 0.7237345 0.6418846 GPR178 G protein-coupled receptor 178 1121 +/- 79 1008 +/- 36 995 +/- 38 980 +/- 56 -0.04548 -0.23516 -0.835181 0.2763983 0.7669644 GPR30 G protein-coupled receptor 30 152 +/- 12 174 +/- 14 119 +/- 12 130 +/- 11 -0.000254 0.2687788 0.509656 0.2010264 0.3384045 GPR44 G protein-coupled receptor 44 233 +/- 18 202 +/- 21 190 +/- 13 171 +/- 16 -0.049682 -0.292564 -0.379645 0.9611111 0.8004753 GPR92 G protein-coupled receptor 92 317 +/- 25 375 +/- 36 247 +/- 23 253 +/- 17 -0.004923 0.2163604 0.827466 0.0131433 0.9423623 GPRASP1 G protein-coupled receptor associated sorting protein 1 4611 +/- 221 4291 +/- 243 3914 +/- 135 3833 +/- 188 -0.006149 -0.353383 -0.736575 0.1285376 0.9705996 GPRASP2 G protein-coupled receptor associated sorting protein 2 5341 +/- 141 5103 +/- 227 4677 +/- 122 4541 +/- 125 -0.00044 -0.399976 -0.453759 0.1456033 0.6818846 GPRC5C G protein-coupled receptor, family C, group 5, member C 245 +/- 13 255 +/- 16 183 +/- 9 174 +/- 13 -2.72E-06 0.6432329 -0.568755 0.9438822 0.8840864 GPX1 glutathione peroxidase 1 2291 +/- 139 2315 +/- 137 1356 +/- 129 1223 +/- 80 -1.64E-07 0.9061546 -0.406245 0.9327754 0.9553075 GPX4 glutathione peroxidase 4 (phospholipid hydroperoxidase) 7963 +/- 464 8464 +/- 561 6679 +/- 145 7234 +/- 220 -0.004326 0.507451 0.071994 0.3572032 0.2390889 GRASP GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein 1081 +/- 146 848 +/- 57 840 +/- 85 627 +/- 53 -0.002364 -0.183818 -0.065668 0.1136651 0.1946781 GRB14 growth factor receptor-bound protein 14 789 +/- 225 1157 +/- 257 347 +/- 87 346 +/- 64 -0.002449 0.306788 -0.987917 0.297082 0.5684402 GRIK1 glutamate receptor, ionotropic, kainate 1 107 +/- 11 355 +/- 42 65 +/- 9 194 +/- 15 -0.005795 0.0015561 0.000176 0.0077755 0.005943 GRINL1A glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A 108 +/- 11 145 +/- 23 83 +/- 9 91 +/- 7 -0.018665 0.1861878 0.486197 0.0767677 0.282887 GRM3 glutamate receptor, metabotropic 3 6089 +/- 758 6114 +/- 726 3163 +/- 219 2897 +/- 273 -0.000172 0.9814705 -0.46892 0.7541802 0.8651269 GRM5 Glutamate receptor, metabotropic 5 4099 +/- 219 3135 +/- 171 3523 +/- 175 2644 +/- 98 -0.054363 -0.006511 -0.00259 0.0064815 0.0858297 GRN granulin 1767 +/- 125 1735 +/- 78 1562 +/- 33 1507 +/- 52 -0.011714 -0.830231 -0.402746 0.4755725 0.154468 GRPEL1 GrpE-like 1, mitochondrial (E. coli) 854 +/- 38 802 +/- 45 690 +/- 34 640 +/- 41 -3.37E-06 -0.400589 -0.374876 0.5356235 0.4308289 GSN gelsolin (amyloidosis, Finnish type) 4180 +/- 541 5269 +/- 560 2845 +/- 206 3577 +/- 214 -0.001124 0.1927344 0.03637 0.2336001 0.1320149 GSTK1 glutathione S-transferase kappa 1 1331 +/- 123 1341 +/- 79 1107 +/- 49 1135 +/- 32 -0.004846 0.9516599 0.641679 0.6371197 0.6072014 GSTO1 glutathione S-transferase omega 1 2518 +/- 351 3310 +/- 538 1226 +/- 48 1372 +/- 69 -0.000434 0.2501203 0.123916 0.4686214 0.3728452 GSTO2 glutathione S-transferase omega 2 193 +/- 11 168 +/- 15 152 +/- 11 142 +/- 5 -0.015265 -0.225592 -0.426157 0.2815143 0.6030164 GSTZ1 glutathione transferase zeta 1 (maleylacetoacetate isomerase) 532 +/- 57 415 +/- 37 407 +/- 38 321 +/- 36 -0.013776 -0.116965 -0.132144 0.1604297 0.5070547 GTF2B general transcription factor IIB 2008 +/- 33 1910 +/- 69 1609 +/- 31 1665 +/- 27 -2.09E-05 -0.23827 0.213192 0.1330927 0.4786923 GTF2H5 general transcription factor IIH, polypeptide 5 1686 +/- 106 1502 +/- 113 1400 +/- 61 1199 +/- 50 -0.002828 -0.260413 -0.030987 0.2897615 0.0226314 GTPBP2 GTP binding protein 2 666 +/- 34 654 +/- 10 583 +/- 19 591 +/- 25 -0.017656 -0.747301 0.805625 0.8757073 0.8779331 GUF1 GUF1 GTPase homolog (S. cerevisiae) 391 +/- 29 526 +/- 32 365 +/- 39 430 +/- 27 -0.017923 0.0116682 0.209153 0.0437322 0.3849475 GUK1 guanylate kinase 1 4058 +/- 348 3405 +/- 159 3210 +/- 51 3114 +/- 124 -0.040578 -0.132199 -0.503028 0.0666274 0.1528928 GUP1 GUP1 glycerol uptake/transporter homolog (S. cerevisiae) 602 +/- 64 1060 +/- 102 467 +/- 55 753 +/- 60 -0.00411 0.0046362 0.006826 0.0049883 0.0567797 H1F0 H1 histone family, member 0 1586 +/- 121 1504 +/- 164 1458 +/- 52 1178 +/- 25 -0.030654 -0.696287 -0.001738 0.4357901 0.0087921 H3F3B H3 histone, family 3B (H3.3B) 8142 +/- 343 8900 +/- 497 7212 +/- 297 7453 +/- 107 -0.004344 0.2417335 0.472215 0.1061159 0.4509122 HADH2 hydroxyacyl-Coenzyme A dehydrogenase, type II 2567 +/- 64 2333 +/- 98 2257 +/- 48 2004 +/- 54 -4.57E-05 -0.078236 -0.007019 0.1175237 0.0859568

S2-genes differentially expressed across region.xls Page 50 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI HADHA hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzy 1139 +/- 62 1306 +/- 61 887 +/- 37 962 +/- 44 -0.000355 0.0832165 0.22687 0.0639413 0.2434309 HADHB hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzy 914 +/- 38 909 +/- 85 750 +/- 24 687 +/- 61 -0.000128 -0.951326 -0.378431 0.7439664 0.237353 HADHSC L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain 894 +/- 53 981 +/- 44 674 +/- 30 650 +/- 26 -6.88E-05 0.2413289 -0.562883 0.0747124 0.8072174 HAGH hydroxyacylglutathione hydrolase 3456 +/- 243 2690 +/- 123 2867 +/- 137 2258 +/- 72 -0.000327 -0.024497 -0.005119 0.0301329 0.0219011 HAGHL hydroxyacylglutathione hydrolase-like 559 +/- 53 491 +/- 35 436 +/- 21 337 +/- 5 -0.000591 -0.315099 -0.004581 0.402573 0.0145572 HAPLN2 hyaluronan and proteoglycan link protein 2 1664 +/- 368 1772 +/- 329 879 +/- 126 1005 +/- 169 -0.010789 0.8324099 0.565409 0.2947258 0.3034251 HARSL histidyl-tRNA synthetase-like 1101 +/- 27 1110 +/- 50 988 +/- 28 1016 +/- 22 -0.0213 0.8816235 0.468098 0.8722715 0.7578004 HBA1 hemoglobin, alpha 1 1624 +/- 541 843 +/- 154 896 +/- 326 696 +/- 162 -0.005345 -0.216078 -0.598393 0.1440203 0.7479856 HBB hemoglobin, beta 8460 +/- 2004 4805 +/- 635 4797 +/- 1700 4794 +/- 780 -0.016388 -0.132731 -0.998774 0.0507226 0.8826689 HBLD1 HESB like domain containing 1 2165 +/- 133 1995 +/- 80 1700 +/- 67 1797 +/- 92 -0.007551 -0.302842 0.417565 0.0987163 0.8563894 HBP1 HMG-box transcription factor 1 1383 +/- 97 1536 +/- 117 1161 +/- 36 1145 +/- 18 -0.00361 0.3376842 -0.695255 0.112367 0.4011065 HBXIP hepatitis B virus x interacting protein 3411 +/- 160 3370 +/- 81 3108 +/- 187 3119 +/- 85 -0.016891 -0.825863 0.958879 0.7114329 0.4852797 HCG12 HLA complex group 12 719 +/- 49 705 +/- 36 557 +/- 26 547 +/- 20 -0.000694 -0.828325 -0.782796 0.7842467 0.782299 HCN1 Hyperpolarization activated cyclic nucleotide-gated potassium channel 1 1694 +/- 66 1549 +/- 133 1568 +/- 145 1286 +/- 110 -0.035824 -0.358955 -0.157183 0.205571 0.2183205 HCN3 hyperpolarization activated cyclic nucleotide-gated potassium channel 3 278 +/- 57 310 +/- 28 222 +/- 23 216 +/- 17 -0.01641 0.6280701 -0.833617 0.7440994 0.8733552 HDAC11 Histone deacetylase 11 3200 +/- 431 3729 +/- 400 2319 +/- 144 2838 +/- 131 -0.009873 0.3894085 0.025646 0.2581662 0.0703184 HDAC5 histone deacetylase 5 2528 +/- 173 2311 +/- 152 1656 +/- 111 1578 +/- 91 -0.000166 -0.368913 -0.597727 0.4153134 0.8951227 HDAC6 histone deacetylase 6 858 +/- 46 1082 +/- 96 763 +/- 14 907 +/- 33 -0.011085 0.0726796 0.008625 0.2408406 0.0768492 HDGF hepatoma-derived growth factor (high-mobility group protein 1-like) 2905 +/- 89 2801 +/- 95 2676 +/- 61 2604 +/- 36 -0.044063 -0.44341 -0.340901 0.3588285 0.7199556 HDHD2 haloacid dehalogenase-like hydrolase domain containing 2 2281 +/- 128 1872 +/- 156 1738 +/- 57 1691 +/- 117 -0.000657 -0.071 -0.729736 0.0663882 0.5656462 HDHD3 haloacid dehalogenase-like hydrolase domain containing 3 439 +/- 18 424 +/- 24 392 +/- 23 384 +/- 14 -0.003649 -0.620855 -0.760103 0.85028 0.6597599 HECA headcase homolog (Drosophila) 2099 +/- 164 2042 +/- 81 1592 +/- 64 1621 +/- 85 -0.002568 -0.766018 0.787575 0.3553691 0.6875549 HECW2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 1877 +/- 76 1823 +/- 130 1536 +/- 76 1304 +/- 73 -0.000251 -0.727012 -0.055461 0.6126667 0.4919653 HERC1 hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-li 3490 +/- 87 3209 +/- 99 3290 +/- 56 3128 +/- 37 -0.051693 -0.059808 -0.04215 0.0071283 0.1525603 HEXB hexosaminidase B (beta polypeptide) 1326 +/- 143 1620 +/- 123 1084 +/- 68 1192 +/- 38 -0.015061 0.1511077 0.204955 0.0606865 0.2903156 HEXIM1 hexamethylene bis-acetamide inducible 1 1258 +/- 39 1102 +/- 16 1185 +/- 37 1098 +/- 42 -0.018271 -0.008471 -0.155924 0.0188297 0.4940762 HIAT1 hippocampus abundant transcript 1 1360 +/- 11 1519 +/- 98 1331 +/- 70 1217 +/- 57 -0.032754 0.1661196 -0.237759 0.1032789 0.435948 HIATL1 hippocampus abundant transcript-like 1 1573 +/- 61 1591 +/- 95 1428 +/- 72 1495 +/- 67 -0.044455 0.8767334 0.511956 0.516753 0.5507297 HIGD1B HIG1 domain family, member 1B 225 +/- 13 268 +/- 11 154 +/- 8 203 +/- 12 -2.08E-05 0.0303291 0.009653 0.0324301 0.0572933 HILS1 histone linker H1 domain, spermatid-specific 1 238 +/- 37 334 +/- 64 129 +/- 13 173 +/- 19 -0.006373 0.2273874 0.101731 0.0512947 0.1278626 HINT2 histidine triad nucleotide binding protein 2 2798 +/- 188 2811 +/- 104 2613 +/- 87 2583 +/- 74 -0.036827 0.9506599 -0.7998 0.8338289 0.9433845 HIP1 Huntingtin interacting protein 1 758 +/- 66 935 +/- 101 589 +/- 42 657 +/- 62 -0.013073 0.1785935 0.391061 0.0841524 0.3668913 HIPK2 Homeodomain interacting protein kinase 2 13628 +/- 1528 17700 +/- 1910 8772 +/- 365 10920 +/- 395 -0.001574 0.1284122 0.003367 0.0120334 0.0028744 HIRIP3 HIRA interacting protein 3 668 +/- 45 749 +/- 24 617 +/- 19 655 +/- 27 -0.022473 0.1529568 0.28509 0.4466955 0.8692679 HIST1H1C histone 1, H1c 322 +/- 49 360 +/- 48 220 +/- 18 248 +/- 23 -0.009955 0.5931834 0.366453 0.39816 0.5588052 HIST1H2BKhistone 1, H2bk 852 +/- 177 597 +/- 60 514 +/- 33 446 +/- 26 -0.047255 -0.22033 -0.139105 0.2745184 0.215615 HKR1 GLI-Kruppel family member HKR1 894 +/- 40 852 +/- 49 803 +/- 44 787 +/- 25 -0.011724 -0.521806 -0.756728 0.8266141 0.746866 HLA-B major histocompatibility complex, class I, B 759 +/- 175 595 +/- 95 510 +/- 116 308 +/- 96 -0.012743 -0.433495 -0.210947 0.6054086 0.6482547 HLA-DMA major histocompatibility complex, class II, DM alpha 100 +/- 12 213 +/- 26 81 +/- 17 132 +/- 17 -0.03817 0.0051739 0.062295 0.0063115 0.1378359 HLA-DPA1 major histocompatibility complex, class II, DP alpha 1 242 +/- 37 469 +/- 34 224 +/- 35 351 +/- 66 -0.052627 0.0011439 0.139667 0.0230307 0.3222405 HLA-DPB1 major histocompatibility complex, class II, DP beta 1 275 +/- 55 485 +/- 42 153 +/- 53 409 +/- 56 -0.000767 0.0138141 0.009355 0.1031803 0.0248301 HLA-DRA major histocompatibility complex, class II, DR alpha 537 +/- 59 893 +/- 114 350 +/- 59 668 +/- 140 -0.022781 0.0254037 0.086285 0.0282195 0.3021055 HLA-E major histocompatibility complex, class I, E 238 +/- 18 323 +/- 43 214 +/- 28 187 +/- 13 -0.018308 0.113861 -0.403342 0.3376283 0.5193864 HMG20B high-mobility group 20B 388 +/- 20 405 +/- 23 354 +/- 12 336 +/- 14 -0.008641 0.6006965 -0.3566 0.4152061 0.4636145 HMHA1 histocompatibility (minor) HA-1 162 +/- 13 218 +/- 28 125 +/- 10 148 +/- 16 -0.005266 0.114584 0.271131 0.2578326 0.8287283

S2-genes differentially expressed across region.xls Page 51 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI HMOX2 heme oxygenase (decycling) 2 2412 +/- 178 2381 +/- 115 1831 +/- 94 2040 +/- 110 -0.00308 -0.888603 0.18397 0.9739114 0.3922139 HNT neurotrimin 2284 +/- 104 2116 +/- 86 1684 +/- 71 1696 +/- 67 -0.000178 -0.242383 0.906694 0.2118724 0.8818579 HOMER1 homer homolog 1 (Drosophila) 2901 +/- 169 2385 +/- 132 2363 +/- 91 2257 +/- 80 -0.023078 -0.038188 -0.408436 0.0448595 0.3022804 HOOK2 hook homolog 2 (Drosophila) 223 +/- 21 209 +/- 15 179 +/- 17 169 +/- 16 -0.003135 -0.616496 -0.673025 0.7207212 0.7091053 HRASLS3 HRAS-like suppressor 3 3691 +/- 429 4571 +/- 653 2117 +/- 93 2770 +/- 179 -0.001697 0.290076 0.017368 0.1218947 0.0152259 HS1BP3 HS1-binding protein 3 323 +/- 18 374 +/- 24 190 +/- 32 362 +/- 29 -0.023458 0.1289462 0.0032 0.3309578 0.0331526 HS3ST1 heparan sulfate (glucosamine) 3-O-sulfotransferase 1 585 +/- 86 446 +/- 58 300 +/- 44 255 +/- 23 -0.000237 -0.2158 -0.394184 0.121015 0.6786008 HS3ST2 heparan sulfate (glucosamine) 3-O-sulfotransferase 2 330 +/- 58 411 +/- 74 170 +/- 26 146 +/- 16 -0.001058 0.4034336 -0.456095 0.5273847 0.672396 HS6ST3 heparan sulfate 6-O-sulfotransferase 3 2846 +/- 592 1913 +/- 169 1347 +/- 145 1363 +/- 47 -0.022947 -0.182153 0.918908 0.0997798 0.9199959 HSBP1 heat shock factor binding protein 1 4920 +/- 366 5203 +/- 167 4384 +/- 209 4328 +/- 203 -0.004956 0.5048028 -0.851416 0.2356832 0.7514638 HSD11B1 hydroxysteroid (11-beta) dehydrogenase 1 182 +/- 39 214 +/- 25 78 +/- 15 136 +/- 13 -0.009558 0.5032791 0.015016 0.5861892 0.0606506 HSD17B6 hydroxysteroid (17-beta) dehydrogenase 6 3312 +/- 301 2181 +/- 99 2842 +/- 167 1796 +/- 97 -0.003708 -0.011557 -0.00067 0.0240631 0.0116791 HSD17B8 hydroxysteroid (17-beta) dehydrogenase 8 953 +/- 84 856 +/- 49 744 +/- 42 752 +/- 43 -0.006774 -0.351849 0.903909 0.5702645 0.8827309 HSP90AB1heat shock protein 90kDa alpha (cytosolic), class B member 1 11071 +/- 195 13271 +/- 831 9909 +/- 375 10714 +/- 222 -0.006433 0.0450899 0.102338 0.1705916 0.0942842 HSPA12B heat shock 70kD protein 12B 255 +/- 35 232 +/- 14 172 +/- 6 171 +/- 17 -0.006819 -0.550858 -0.952264 0.8933895 0.614376 HSPB1 heat shock 27kDa protein 1 3278 +/- 248 3121 +/- 237 2296 +/- 145 2560 +/- 172 -0.002544 -0.656623 0.2722 0.8494225 0.4187507 HSPB8 heat shock 22kDa protein 8 2202 +/- 213 3231 +/- 299 2037 +/- 225 2797 +/- 223 -0.014424 0.0205075 0.040316 0.0801769 0.3845944 HSPBP1 hsp70-interacting protein 2962 +/- 168 2490 +/- 168 2343 +/- 76 2141 +/- 114 -0.003381 -0.074945 -0.183278 0.0120651 0.2457536 HSPC142 HSPC142 protein 2272 +/- 130 2255 +/- 86 1858 +/- 67 1924 +/- 27 -0.000137 -0.916832 0.390418 0.8570846 0.2506358 HSPC171 HSPC171 protein 1285 +/- 10 1228 +/- 28 1081 +/- 40 1118 +/- 47 -0.000576 -0.102422 0.565018 0.0043881 0.8704792 HSPE1 heat shock 10kDa protein 1 (chaperonin 10) 3558 +/- 135 3064 +/- 138 3300 +/- 90 2930 +/- 94 -0.054454 -0.028255 -0.019469 0.0466405 0.0305899 HTLF human T-cell leukemia virus enhancer factor 3074 +/- 294 3758 +/- 466 2275 +/- 114 2457 +/- 173 -0.010047 0.2475937 0.406503 0.0371805 0.4634845 HVCN1 hydrogen voltage-gated channel 1 468 +/- 71 398 +/- 35 317 +/- 23 306 +/- 29 -0.030419 -0.403404 -0.773217 0.8073495 0.452806 HYLS1 hydrolethalus syndrome 1 748 +/- 26 632 +/- 33 635 +/- 12 572 +/- 47 -0.002791 -0.020014 -0.253758 0.0580063 0.9635505 HYPK Huntingtin interacting protein K 2194 +/- 146 2010 +/- 57 1779 +/- 84 1712 +/- 99 -0.002484 -0.282399 -0.620898 0.4431515 0.927486 IAPP Islet amyloid polypeptide 535 +/- 38 484 +/- 41 411 +/- 29 428 +/- 44 -0.001318 -0.373448 0.763409 0.1390971 0.8839214 ICAM3 intercellular adhesion molecule 3 168 +/- 7 185 +/- 9 159 +/- 13 159 +/- 12 -0.019841 0.1685375 0.979403 0.070669 0.5196951 ID1 inhibitor of DNA binding 1, dominant negative helix-loop-helix protein 394 +/- 73 443 +/- 59 308 +/- 32 282 +/- 20 -0.002975 0.6127017 -0.500074 0.8671656 0.3788378 IDE insulin-degrading enzyme 665 +/- 21 696 +/- 35 558 +/- 17 572 +/- 22 -0.000173 0.4716953 0.608086 0.1372707 0.522891 IDH3B isocitrate dehydrogenase 3 (NAD+) beta 1428 +/- 48 1204 +/- 29 1103 +/- 38 1036 +/- 36 -0.00011 -0.003798 -0.233414 0.0286207 0.7010248 IDH3G isocitrate dehydrogenase 3 (NAD+) gamma 2628 +/- 270 2453 +/- 134 1997 +/- 71 2177 +/- 82 -0.029348 -0.580233 0.133656 0.3381091 0.5473444 IER2 immediate early response 2 599 +/- 65 576 +/- 35 507 +/- 24 470 +/- 25 -0.024213 -0.768513 -0.320357 0.7803853 0.4800627 IFI16 interferon, gamma-inducible protein 16 330 +/- 19 339 +/- 20 237 +/- 14 240 +/- 21 -0.000715 0.7261972 0.890853 0.2966608 0.7748373 IFI44 interferon-induced protein 44 261 +/- 30 265 +/- 25 175 +/- 21 215 +/- 19 -0.001606 0.9087625 0.194834 0.3857896 0.2358217 IFIT3 interferon-induced protein with tetratricopeptide repeats 3 309 +/- 18 383 +/- 25 287 +/- 18 321 +/- 15 -0.051765 0.0397857 0.17307 0.0204367 0.3699558 IFITM1 interferon induced transmembrane protein 1 (9-27) 833 +/- 79 879 +/- 83 590 +/- 60 753 +/- 89 -0.000503 0.6952804 0.17386 0.755764 0.0403564 IFITM2 interferon induced transmembrane protein 2 (1-8D) 839 +/- 59 783 +/- 114 679 +/- 81 657 +/- 78 -0.00023 -0.675757 -0.850529 0.6366633 0.9723548 IFNAR1 interferon (alpha, beta and omega) receptor 1 514 +/- 18 825 +/- 71 497 +/- 23 630 +/- 59 -0.027489 0.0063634 0.088126 0.0011211 0.1302925 IFNGR1 interferon gamma receptor 1 1775 +/- 52 2011 +/- 102 1350 +/- 54 1527 +/- 56 -0.000166 0.0756206 0.049648 0.0313991 0.0954485 IFP38 IFP38 3530 +/- 175 3705 +/- 99 3217 +/- 84 3297 +/- 106 -0.001233 0.411997 0.569433 0.6136826 0.7105462 IGBP1 immunoglobulin (CD79A) binding protein 1 1965 +/- 47 2506 +/- 69 1885 +/- 71 1954 +/- 88 -0.021218 0.0001316 0.556128 0.0088608 0.3758762 IGFBP4 insulin-like growth factor binding protein 4 517 +/- 34 422 +/- 31 372 +/- 29 376 +/- 25 -0.01523 -0.065764 0.918885 0.0142596 0.4116001 IGFBP6 insulin-like growth factor binding protein 6 795 +/- 47 804 +/- 66 655 +/- 38 750 +/- 54 -0.0153 0.9125309 0.191107 0.255318 0.8540409 IGFBP7 insulin-like growth factor binding protein 7 1047 +/- 88 816 +/- 52 766 +/- 62 678 +/- 70 -0.001545 -0.078309 -0.372074 0.0422431 0.4657143 IGKC immunoglobulin kappa constant 214 +/- 51 211 +/- 12 144 +/- 11 165 +/- 19 -0.034349 -0.964313 0.372452 0.8438192 0.1970326

S2-genes differentially expressed across region.xls Page 52 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI IGSF11 immunoglobulin superfamily, member 11 1835 +/- 127 2330 +/- 191 1702 +/- 60 1916 +/- 70 -0.044071 0.0602246 0.048375 0.0127626 0.0923113 IGSF21 immunoglobin superfamily, member 21 2589 +/- 227 2116 +/- 77 1428 +/- 26 1159 +/- 124 -6.95E-05 -0.094198 -0.095985 0.0461055 0.0281133 IGSF4 immunoglobulin superfamily, member 4 2000 +/- 94 2276 +/- 143 1619 +/- 80 1666 +/- 98 -4.65E-05 0.1427333 0.72063 0.0269001 0.4254562 IKBKAP inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated p 3512 +/- 159 3207 +/- 197 3071 +/- 108 2708 +/- 98 -0.000191 -0.257208 -0.034273 0.9879113 0.3691163 IL10RA interleukin 10 receptor, alpha 173 +/- 20 220 +/- 18 140 +/- 11 160 +/- 8 -0.0117 0.1100806 0.176611 0.1045609 0.2012281 ILKAP integrin-linked kinase-associated serine/threonine phosphatase 2C 587 +/- 52 550 +/- 29 427 +/- 29 451 +/- 37 -0.005466 -0.552223 0.624437 0.7625935 0.5441965 IMP3 IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast) 1494 +/- 106 1345 +/- 112 1151 +/- 48 1116 +/- 91 -0.00046 -0.356686 -0.744279 0.2216162 0.1764377 IMP4 IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) 525 +/- 30 464 +/- 18 434 +/- 20 444 +/- 32 -0.018635 -0.120641 0.805872 0.0058275 0.3863858 INPP5F inositol polyphosphate-5-phosphatase F 7316 +/- 853 6772 +/- 702 4777 +/- 305 4323 +/- 133 -0.000705 -0.633408 -0.216146 0.5046604 0.3041385 INTS4 integrator complex subunit 4 572 +/- 27 670 +/- 75 472 +/- 18 543 +/- 33 -0.005617 0.2655561 0.104248 0.0175688 0.0102469 INTS8 integrator complex subunit 8 550 +/- 35 533 +/- 17 506 +/- 26 471 +/- 20 -0.01725 -0.680762 -0.319143 0.6574837 0.5907708 IQGAP1 IQ motif containing GTPase activating protein 1 930 +/- 56 1158 +/- 88 871 +/- 23 882 +/- 53 -0.035868 0.0582734 0.850847 0.1777142 0.7626615 IRF8 interferon regulatory factor 8 319 +/- 24 330 +/- 47 252 +/- 22 217 +/- 19 -0.006048 0.8430084 -0.25384 0.2365138 0.5420586 IRS2 insulin receptor substrate 2 5710 +/- 303 5168 +/- 167 4725 +/- 171 4676 +/- 173 -0.011047 -0.156353 -0.843904 0.0887965 0.945802 ISG15 ISG15 ubiquitin-like modifier 312 +/- 25 375 +/- 30 216 +/- 17 298 +/- 16 -0.001794 0.1427383 0.007132 0.1085195 0.2043022 ISGF3G interferon-stimulated transcription factor 3, gamma 48kDa 643 +/- 47 741 +/- 40 580 +/- 32 592 +/- 32 -0.022285 0.1460074 0.785364 0.0443342 0.95397 ISOC2 isochorismatase domain containing 2 245 +/- 16 247 +/- 20 230 +/- 13 201 +/- 10 -0.032999 0.9599469 -0.124688 0.5387185 0.1182203 ITFG1 Integrin alpha FG-GAP repeat containing 1 7600 +/- 215 6251 +/- 221 6538 +/- 483 6093 +/- 241 -0.020899 -0.001403 -0.437198 0.0083954 0.6222976 ITGA9 integrin, alpha 9 349 +/- 37 250 +/- 36 173 +/- 17 170 +/- 14 -7.19E-05 -0.086134 -0.900995 0.048457 0.7015164 ITGB4BP integrin beta 4 binding protein 1162 +/- 72 1267 +/- 32 1073 +/- 45 1031 +/- 73 -0.007018 0.2212276 -0.641713 0.03942 0.5366645 IVNS1ABP influenza virus NS1A binding protein 993 +/- 80 1097 +/- 80 745 +/- 17 848 +/- 36 -0.000147 0.3783459 0.043123 0.2703699 0.0029402 JMJD1A jumonji domain containing 1A 959 +/- 56 908 +/- 41 794 +/- 33 787 +/- 38 -0.000306 -0.476108 -0.896091 0.5363759 0.7586733 JMJD1B jumonji domain containing 1B 1494 +/- 105 1603 +/- 48 1306 +/- 121 1285 +/- 64 -0.000322 0.3740458 -0.883392 0.6168754 0.4767708 JOSD1 Josephin domain containing 1 2148 +/- 113 1993 +/- 62 1909 +/- 91 1796 +/- 58 -0.007044 -0.265239 -0.326716 0.3934449 0.6376179 JTB jumping translocation breakpoint 5670 +/- 182 5899 +/- 212 5330 +/- 174 5451 +/- 225 -0.000163 0.433297 0.682823 0.1347663 0.3245097 JTV1 JTV1 gene 1612 +/- 83 1452 +/- 88 1310 +/- 38 1223 +/- 42 -9.22E-05 -0.215755 -0.160704 0.2667909 0.1489044 KBTBD3 kelch repeat and BTB (POZ) domain containing 3 245 +/- 17 231 +/- 14 224 +/- 11 186 +/- 9 -0.00714 -0.559314 -0.027498 0.8803297 0.0445753 KCNF1 potassium voltage-gated channel, subfamily F, member 1 1301 +/- 149 1157 +/- 130 914 +/- 59 867 +/- 82 -0.004603 -0.482317 -0.651226 0.1991061 0.6935352 KCNK9 CDNA FLJ37082 fis, clone BRACE2016465 443 +/- 74 291 +/- 34 131 +/- 23 109 +/- 16 -0.000195 -0.103079 -0.451343 0.0532054 0.4321239 KEAP1 kelch-like ECH-associated protein 1 1142 +/- 76 1174 +/- 47 1028 +/- 40 969 +/- 25 -0.008321 0.7272021 -0.244847 0.7175914 0.4381657 KHDRBS1 KH domain containing, RNA binding, signal transduction associated 1 434 +/- 12 512 +/- 23 424 +/- 28 401 +/- 15 -0.035547 0.0168749 -0.478305 0.3175819 0.3900469 KHDRBS2 KH domain containing, RNA binding, signal transduction associated 2 174 +/- 28 148 +/- 14 118 +/- 18 128 +/- 8 -0.001609 -0.442173 0.634274 0.2318912 0.6807588 KIDINS220Kinase D-interacting substance of 220 kDa 221 +/- 98 520 +/- 82 208 +/- 72 376 +/- 75 -0.033925 0.0420293 0.142025 0.1127798 0.3116226 KIF13B kinesin family member 13B 1459 +/- 204 1815 +/- 246 960 +/- 78 1028 +/- 71 -0.00386 0.2921502 0.536881 0.083335 0.1896473 KIF1B kinesin family member 1B 9860 +/- 467 9016 +/- 271 9037 +/- 492 8493 +/- 275 -0.039808 -0.156672 -0.364326 0.4949853 0.4732774 KIF3C kinesin family member 3C 5625 +/- 458 4054 +/- 161 4195 +/- 251 3767 +/- 99 -0.012429 -0.016957 -0.16042 0.0051181 0.2302338 KIF5A kinesin family member 5A 2488 +/- 627 4967 +/- 527 2216 +/- 519 3839 +/- 250 -0.025483 0.0131554 0.025515 0.0698021 0.1681597 KIF5C kinesin family member 5C 16153 +/- 387 17048 +/- 555 15496 +/- 754 15176 +/- 641 -0.028021 0.2185443 -0.753928 0.2349383 0.7855717 KIF6 kinesin family member 6 175 +/- 12 141 +/- 19 112 +/- 8 115 +/- 10 -0.020904 -0.161401 0.824485 0.9758413 0.2624701 KLC4 kinesin light chain 4 771 +/- 46 694 +/- 18 672 +/- 36 597 +/- 40 -0.002145 -0.168056 -0.195756 0.1193463 0.0819752 KLF5 Kruppel-like factor 5 (intestinal) 174 +/- 48 201 +/- 58 98 +/- 21 95 +/- 20 -0.016261 0.7232551 -0.928849 0.7592427 0.7340477 KLHDC2 kelch domain containing 2 4166 +/- 301 4530 +/- 181 3483 +/- 181 3652 +/- 131 -0.0002 0.3289498 0.471012 0.2628451 0.2056535 KLHDC3 kelch domain containing 3 8738 +/- 505 7977 +/- 393 6742 +/- 137 6563 +/- 102 -0.000354 -0.263576 -0.321858 0.0438481 0.2491643 KLHL13 kelch-like 13 (Drosophila) 604 +/- 100 498 +/- 54 385 +/- 35 394 +/- 25 -0.025034 -0.376511 0.8452 0.3114399 0.8925399 KLHL17 kelch-like 17 (Drosophila) 368 +/- 27 349 +/- 13 321 +/- 16 298 +/- 17 -0.013291 -0.528632 -0.354492 0.303531 0.1302696

S2-genes differentially expressed across region.xls Page 53 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI KLHL21 kelch-like 21 (Drosophila) 1952 +/- 122 1902 +/- 103 1702 +/- 32 1533 +/- 45 -0.00605 -0.762969 -0.016513 0.7908324 0.0086259 KLK6 kallikrein 6 (neurosin, zyme) 1030 +/- 190 1991 +/- 422 602 +/- 104 823 +/- 47 -0.014244 0.07681 0.094055 0.0120377 0.0600326 KRT17 keratin 17 472 +/- 164 400 +/- 85 125 +/- 17 128 +/- 10 -0.004346 -0.707531 0.894511 0.810175 0.9343139 Kua ubiquitin-conjugating enzyme variant Kua 968 +/- 77 876 +/- 70 858 +/- 38 702 +/- 42 -0.020679 -0.400916 -0.022812 0.6755094 0.0203844 LAMP1 lysosomal-associated membrane protein 1 4569 +/- 324 5261 +/- 423 3749 +/- 165 4315 +/- 199 -0.019346 0.225437 0.059178 0.1044209 0.2230529 LAMP2 lysosomal-associated membrane protein 2 1002 +/- 127 1448 +/- 245 598 +/- 83 745 +/- 47 -0.00608 0.1463506 0.164417 0.0226136 0.1815686 LANCL1 LanC lantibiotic synthetase component C-like 1 (bacterial) 3537 +/- 248 3724 +/- 361 2526 +/- 76 3336 +/- 364 -0.034458 0.6797791 0.089243 0.2045664 0.1855416 LANCL2 LanC lantibiotic synthetase component C-like 2 (bacterial) 2838 +/- 540 2166 +/- 175 1623 +/- 103 1597 +/- 88 -0.017856 -0.280338 -0.854056 0.2394972 0.5946485 LARP5 La ribonucleoprotein domain family, member 5 1526 +/- 96 1282 +/- 61 1338 +/- 49 1181 +/- 56 -0.042165 -0.062159 -0.065877 0.2175997 0.4722559 LARP6 La ribonucleoprotein domain family, member 6 1822 +/- 122 1949 +/- 164 1462 +/- 121 1546 +/- 147 -0.033311 0.5508393 0.668272 0.2070976 0.6854497 LASS2 LAG1 longevity assurance homolog 2 (S. cerevisiae) 1543 +/- 159 2019 +/- 247 1012 +/- 58 1354 +/- 79 -0.005517 0.1417419 0.008589 0.0146985 0.0166976 LAYN layilin 174 +/- 39 137 +/- 9 89 +/- 5 120 +/- 9 -0.030574 -0.392233 0.018567 0.164778 0.2289666 LCP2 lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) 200 +/- 23 250 +/- 30 148 +/- 13 171 +/- 24 -0.000379 0.2152897 0.44138 0.050337 0.1261438 LDB3 LIM domain binding 3 241 +/- 39 397 +/- 60 173 +/- 29 191 +/- 12 -0.014245 0.0600527 0.571194 0.0353184 0.922557 LDHA lactate dehydrogenase A 7428 +/- 363 7503 +/- 464 5839 +/- 323 6507 +/- 427 -7.99E-06 0.9005206 0.248174 0.3634801 0.9661517 LDHB lactate dehydrogenase B 21336 +/- 581 20009 +/- 863 18771 +/- 407 19615 +/- 1000 -0.048622 -0.234781 0.467554 0.0687089 0.8661576 LDOC1L leucine zipper, down-regulated in cancer 1-like 2517 +/- 82 2439 +/- 61 2312 +/- 80 2272 +/- 68 -0.000218 -0.463737 -0.713159 0.3506474 0.9529308 LGALS1 lectin, galactoside-binding, soluble, 1 (galectin 1) 1561 +/- 151 2182 +/- 457 1000 +/- 65 1126 +/- 127 -0.001133 0.2440003 0.41052 0.5646307 0.8880205 LGI3 leucine-rich repeat LGI family, member 3 1933 +/- 374 2190 +/- 318 1012 +/- 99 1060 +/- 37 -0.001907 0.6124157 0.665252 0.4292039 0.4346088 LGTN ligatin 798 +/- 26 801 +/- 41 551 +/- 26 581 +/- 26 -7.4E-06 0.9444288 0.417883 0.8889981 0.5572322 LHFPL3 lipoma HMGIC fusion partner-like 3 1093 +/- 129 955 +/- 73 793 +/- 54 630 +/- 52 -0.002826 -0.380638 -0.058136 0.8018992 0.119491 LHPP phospholysine phosphohistidine inorganic pyrophosphate phosphatase 1897 +/- 163 2175 +/- 138 1606 +/- 105 1935 +/- 93 -0.035074 0.2232212 0.043902 0.1423172 0.2170396 LIFR leukemia inhibitory factor receptor alpha 2392 +/- 272 3256 +/- 164 2200 +/- 139 2374 +/- 110 -0.023868 0.025646 0.350575 0.0182029 0.8117387 LIMS1 LIM and senescent cell antigen-like domains 1 815 +/- 63 1059 +/- 110 676 +/- 38 871 +/- 66 -0.017507 0.0901478 0.040073 0.0174023 0.0048156 LIN28B lin-28 homolog B (C. elegans) 158 +/- 30 129 +/- 16 60 +/- 14 54 +/- 5 -7.78E-05 -0.417165 -0.687115 0.566961 0.5086811 LIN7B lin-7 homolog B (C. elegans) 1185 +/- 89 1059 +/- 44 994 +/- 42 1035 +/- 52 -0.037835 -0.246077 0.558555 0.1378564 0.6800087 LIPE lipase, hormone-sensitive 641 +/- 74 906 +/- 109 439 +/- 29 475 +/- 22 -0.004068 0.0768589 0.355349 0.022113 0.3041516 LITAF lipopolysaccharide-induced TNF factor 1951 +/- 250 2975 +/- 472 1460 +/- 69 1769 +/- 112 -0.014996 0.0932603 0.052765 0.0133063 0.013689 LMAN2L lectin, mannose-binding 2-like 532 +/- 54 623 +/- 35 497 +/- 50 581 +/- 22 -0.047312 0.1923099 0.169516 0.1113651 0.2193227 LMCD1 LIM and cysteine-rich domains 1 535 +/- 76 697 +/- 87 307 +/- 25 374 +/- 28 -0.001364 0.189184 0.10736 0.1164308 0.4521707 LMO3 LIM domain only 3 (rhombotin-like 2) 9466 +/- 961 9899 +/- 747 7430 +/- 667 7767 +/- 555 -0.028017 0.7296423 0.706493 0.9104439 0.5982932 LOC129285smooth muscle myosin heavy chain 11 isoform SM1-like 2168 +/- 172 2422 +/- 230 1473 +/- 60 1606 +/- 80 -0.000487 0.3998912 0.221625 0.2429813 0.021305 LOC153222adult retina protein 3642 +/- 199 3900 +/- 259 3038 +/- 147 3074 +/- 77 -0.000141 0.4486248 0.831885 0.4802418 0.6970846 LOC285442mitochondrial translational release factor 1-like pseudogene 834 +/- 74 851 +/- 65 706 +/- 59 744 +/- 66 -0.000782 0.8668399 0.677212 0.7024378 0.862232 LOC338328high density lipoprotein-binding protein 121 +/- 14 120 +/- 25 78 +/- 6 95 +/- 15 -0.00164 -0.994323 0.341684 0.8770398 0.2069446 LOC401152HCV F-transactivated protein 1 1726 +/- 109 1938 +/- 113 1345 +/- 93 1558 +/- 63 -0.000176 0.2078518 0.092326 0.2490615 0.3522363 LOC84661 dpy-30-like protein 1971 +/- 93 1783 +/- 56 1614 +/- 43 1602 +/- 77 -0.000169 -0.119618 -0.897007 0.1204572 0.8002153 LONRF1 LON peptidase N-terminal domain and ring finger 1 1782 +/- 86 1651 +/- 145 1231 +/- 68 1230 +/- 61 -5.1E-05 -0.458125 -0.990484 0.3359009 0.7337757 LPAAT-TH lysophosphatidic acid acyltransferase theta 238 +/- 53 232 +/- 51 114 +/- 24 108 +/- 16 -0.00153 -0.939118 -0.83364 0.8852671 0.8686009 LPHN3 latrophilin 3 1577 +/- 209 1274 +/- 54 1121 +/- 48 1025 +/- 80 -0.0177 -0.213786 -0.339531 0.3249337 0.7274178 LRP4 low density lipoprotein receptor-related protein 4 2654 +/- 217 2898 +/- 123 2313 +/- 52 2144 +/- 59 -0.004045 0.3572511 -0.063176 0.3310908 0.0511113 LRP6 low density lipoprotein receptor-related protein 6 1780 +/- 105 1710 +/- 106 1436 +/- 55 1341 +/- 57 -0.002563 -0.65083 -0.261352 0.6444996 0.1469601 LRPAP1 low density lipoprotein receptor-related protein associated protein 1 2078 +/- 142 1823 +/- 50 1654 +/- 103 1679 +/- 49 -0.009176 -0.140406 0.836413 0.1308419 0.8635857 LRRC1 leucine rich repeat containing 1 137 +/- 19 178 +/- 20 100 +/- 6 121 +/- 14 -0.014943 0.175425 0.219422 0.1256817 0.6373534 LRRC20 leucine rich repeat containing 20 662 +/- 29 640 +/- 23 578 +/- 22 536 +/- 14 -0.001795 -0.565524 -0.150319 0.5329298 0.1103759

S2-genes differentially expressed across region.xls Page 54 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI LRRC32 leucine rich repeat containing 32 129 +/- 13 156 +/- 8 93 +/- 6 108 +/- 13 -0.000726 0.1021982 0.353141 0.2486034 0.3838753 LRRC47 leucine rich repeat containing 47 2563 +/- 274 2433 +/- 179 2180 +/- 53 2020 +/- 78 -0.038199 -0.701014 -0.131984 0.4837557 0.0121734 LRRC54 leucine rich repeat containing 54 154 +/- 32 174 +/- 20 97 +/- 17 124 +/- 11 -0.016741 0.6045747 0.214596 0.8435089 0.4677715 LRRC8B leucine rich repeat containing 8 family, member B 165 +/- 19 124 +/- 18 104 +/- 13 95 +/- 16 -0.014938 -0.154948 -0.668965 0.5064695 0.5853261 LRRC8C Leucine rich repeat containing 8 family, member C 232 +/- 33 400 +/- 51 193 +/- 36 225 +/- 20 -0.039294 0.0233036 0.464991 0.0011553 0.528369 LRRK2 leucine-rich repeat kinase 2 281 +/- 21 330 +/- 23 245 +/- 15 258 +/- 13 -0.007971 0.1495882 0.529988 0.0860688 0.3099626 LRRTM2 leucine rich repeat transmembrane neuronal 2 2543 +/- 91 2640 +/- 129 2308 +/- 69 2200 +/- 88 -0.000245 0.556089 -0.360835 0.5470758 0.7243256 LSM1 LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae) 1866 +/- 40 1812 +/- 65 1690 +/- 49 1703 +/- 77 -0.008504 -0.504871 0.889677 0.1254078 0.796149 LSM10 LSM10, U7 small nuclear RNA associated 1379 +/- 69 1430 +/- 27 1268 +/- 42 1360 +/- 21 -0.006979 0.5221094 0.08598 0.8484129 0.349762 LSM7 LSM7 homolog, U6 small nuclear RNA associated (S. cerevisiae) 1460 +/- 112 1295 +/- 74 952 +/- 37 920 +/- 33 -3.08E-05 -0.252411 -0.545703 0.4674008 0.8706011 LTB lymphotoxin beta (TNF superfamily, member 3) 150 +/- 32 249 +/- 30 143 +/- 12 161 +/- 19 -0.05276 0.0471031 0.446585 0.0280837 0.4278686 LTB4DH leukotriene B4 12-hydroxydehydrogenase 163 +/- 30 136 +/- 16 81 +/- 15 87 +/- 11 -0.000898 -0.445351 0.763637 0.321739 0.8510308 LTC4S leukotriene C4 synthase 236 +/- 25 314 +/- 22 201 +/- 17 191 +/- 16 -0.013328 0.0419076 -0.662254 0.0265734 0.2643918 LTV1 LTV1 homolog (S. cerevisiae) 825 +/- 67 855 +/- 48 722 +/- 39 741 +/- 17 -0.029719 0.7280491 0.6765 0.8758578 0.7599985 LUZP2 Leucine zipper protein 2 148 +/- 21 101 +/- 16 116 +/- 17 69 +/- 8 -0.002594 -0.10366 -0.035562 0.0774908 0.0654404 LXN latexin 599 +/- 97 409 +/- 66 350 +/- 98 211 +/- 10 -0.012741 -0.141497 -0.216458 0.1632218 0.2473691 LYZ lysozyme (renal amyloidosis) 354 +/- 21 541 +/- 71 247 +/- 38 330 +/- 29 -0.002236 0.045574 0.117092 0.0080013 0.1612533 MAD2L1BPMAD2L1 binding protein 801 +/- 48 804 +/- 14 744 +/- 14 700 +/- 10 -0.014653 0.9520305 -0.030987 0.8433146 0.1503947 MAF1 MAF1 homolog (S. cerevisiae) 1795 +/- 125 1939 +/- 131 1613 +/- 29 1593 +/- 37 -0.025973 0.4467837 -0.679652 0.4519663 0.5465594 MAFB v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) 407 +/- 19 391 +/- 12 309 +/- 17 288 +/- 20 -9.97E-05 -0.463725 -0.456781 0.9044338 0.9732484 MAN1A1 Mannosidase, alpha, class 1A, member 1 4011 +/- 616 2920 +/- 214 2376 +/- 276 2392 +/- 134 -0.037782 -0.143866 0.960428 0.1492796 0.8338358 MAN2A1 Mannosidase, alpha, class 2A, member 1 1559 +/- 217 2028 +/- 286 855 +/- 94 1116 +/- 57 -0.000591 0.2219283 0.044273 0.1878816 0.1437013 MAN2B1 mannosidase, alpha, class 2B, member 1 373 +/- 23 415 +/- 33 331 +/- 18 351 +/- 25 -0.005078 0.3287767 0.514351 0.4867223 0.9270466 MAN2B2 mannosidase, alpha, class 2B, member 2 622 +/- 44 583 +/- 25 586 +/- 54 558 +/- 29 -0.037604 -0.453454 -0.67163 0.3663965 0.5735695 MANEAL mannosidase, endo-alpha-like 2351 +/- 85 2004 +/- 64 2117 +/- 72 1985 +/- 75 -0.041567 -0.009304 -0.239499 0.0043888 0.1250339 MAOA monoamine oxidase A 1504 +/- 108 1546 +/- 51 1232 +/- 100 1314 +/- 125 -0.002127 0.7371193 0.622807 0.6924609 0.6597459 MAP1LC3Bmicrotubule-associated protein 1 light chain 3 beta 4795 +/- 243 5232 +/- 261 4483 +/- 110 4532 +/- 142 -0.011572 0.2487294 0.792139 0.2662723 0.9466995 MAP2K5 mitogen-activated protein kinase kinase 5 569 +/- 16 605 +/- 36 481 +/- 12 468 +/- 17 -0.001265 0.3924884 -0.5345 0.2242973 0.3052941 MAP3K13 Mitogen-activated protein kinase kinase kinase 13 655 +/- 36 806 +/- 40 539 +/- 37 736 +/- 36 -0.003978 0.0192338 0.004278 0.1930077 0.0880737 MAP3K6 mitogen-activated protein kinase kinase kinase 6 292 +/- 47 544 +/- 98 200 +/- 27 272 +/- 34 -0.006841 0.0514709 0.136153 0.0664212 0.0156765 MAP3K7IP mitogen-activated protein kinase kinase kinase 7 interacting protein 2 2168 +/- 80 2360 +/- 68 2014 +/- 52 1942 +/- 32 -0.002022 0.0969371 -0.274435 0.0601452 0.1897326 MAP4 microtubule-associated protein 4 1385 +/- 222 1637 +/- 169 1048 +/- 61 1256 +/- 99 -0.047768 0.3892734 0.119411 0.4555047 0.2092243 MAP4K4 mitogen-activated protein kinase kinase kinase kinase 4 1103 +/- 94 1434 +/- 119 899 +/- 30 954 +/- 55 -0.002764 0.0546811 0.40866 0.0191518 0.1934536 MAP6D1 MAP6 domain containing 1 975 +/- 44 1056 +/- 109 825 +/- 77 876 +/- 64 -0.042112 0.5100763 0.617977 0.1256705 0.7458617 MAPBPIP mitogen-activated protein-binding protein-interacting protein 1203 +/- 34 1132 +/- 25 984 +/- 45 1014 +/- 9 -0.002263 -0.12357 0.539067 0.0444964 0.502111 MAPK6 mitogen-activated protein kinase 6 3225 +/- 189 3472 +/- 168 2883 +/- 80 3049 +/- 106 -0.024034 0.3531164 0.247569 0.4862643 0.3953045 MAPRE1 microtubule-associated protein, RP/EB family, member 1 1826 +/- 135 2236 +/- 182 1327 +/- 73 1595 +/- 31 -0.000328 0.1033306 0.012844 0.0227255 0.0289238 MARCH3 membrane-associated ring finger (C3HC4) 3 241 +/- 17 273 +/- 16 191 +/- 8 193 +/- 5 -0.00087 0.2060585 0.910708 0.123276 0.3711175 MARCH6 membrane-associated ring finger (C3HC4) 6 1489 +/- 19 1154 +/- 34 1345 +/- 50 1122 +/- 25 -0.028684 -2.87E-05 -0.004757 0.0050401 0.0443174 MARCH8 membrane-associated ring finger (C3HC4) 8 1386 +/- 109 1563 +/- 162 1150 +/- 65 1225 +/- 86 -0.031534 0.3894667 0.51011 0.2367577 0.6708436 MARCKSL MARCKS-like 1 6758 +/- 771 8650 +/- 1092 4059 +/- 216 4873 +/- 366 -0.001131 0.190565 0.09974 0.1156372 0.3063554 MASA E-1 enzyme 3744 +/- 190 3956 +/- 117 3375 +/- 91 3432 +/- 96 -0.005682 0.3700109 0.678661 0.4517469 0.5713322 MAWBP MAWD binding protein 1195 +/- 55 1390 +/- 158 1024 +/- 61 1009 +/- 69 -0.003381 0.2860165 -0.868038 0.0310123 0.4493709 MBD5 methyl-CpG binding domain protein 5 1291 +/- 137 1781 +/- 99 1091 +/- 72 1304 +/- 87 -0.00565 0.0174222 0.094876 0.0314458 0.2979001 MBP myelin basic protein 267 +/- 30 382 +/- 30 198 +/- 26 233 +/- 24 -0.002067 0.0221518 0.35689 0.1632095 0.6588283

S2-genes differentially expressed across region.xls Page 55 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI MCAM melanoma cell adhesion molecule 1170 +/- 212 1662 +/- 293 589 +/- 48 817 +/- 61 -0.004321 0.2075345 0.018798 0.0884724 0.0316572 MCM6 MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisia 573 +/- 51 487 +/- 39 454 +/- 18 431 +/- 31 -0.002683 -0.212274 -0.542778 0.2214485 0.9865348 MCOLN1 mucolipin 1 869 +/- 34 752 +/- 20 733 +/- 41 707 +/- 21 -0.001182 -0.019151 -0.582762 0.1177468 0.6944621 MDH1 malate dehydrogenase 1, NAD (soluble) 16855 +/- 421 16112 +/- 725 14018 +/- 537 15178 +/- 746 -0.002053 -0.401764 0.244125 0.0795962 0.7230242 MDH1B malate dehydrogenase 1B, NAD (soluble) 438 +/- 14 356 +/- 17 295 +/- 17 277 +/- 4 -0.000321 -0.004634 -0.33135 0.064732 0.4103282 MDS032 uncharacterized hematopoietic stem/progenitor cells protein MDS032 676 +/- 40 751 +/- 22 602 +/- 16 590 +/- 19 -0.001719 0.1423633 -0.639749 0.1893594 0.4052906 MED10 mediator of RNA polymerase II transcription, subunit 10 homolog (NUT2, S. cerevisiae) 798 +/- 37 769 +/- 26 608 +/- 25 637 +/- 42 -0.000222 -0.540207 0.565305 0.2684865 0.6725126 MED11 mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) 413 +/- 31 455 +/- 22 346 +/- 14 349 +/- 16 -0.006952 0.3035423 0.908648 0.1470224 0.8164084 MED19 mediator of RNA polymerase II transcription, subunit 19 homolog (S. cerevisiae) 1426 +/- 32 1306 +/- 36 1307 +/- 44 1177 +/- 45 -0.003276 -0.032648 -0.069495 0.2870689 0.2965897 MED31 mediator of RNA polymerase II transcription, subunit 31 homolog (S. cerevisiae) 3069 +/- 150 2753 +/- 153 2514 +/- 53 2339 +/- 71 -0.002892 -0.171528 -0.084412 0.1931007 0.4984009 MEGF10 multiple EGF-like-domains 10 775 +/- 88 1122 +/- 99 648 +/- 44 768 +/- 24 -0.025721 0.0259818 0.045696 0.0129927 0.2333869 MEIS1 Meis1, myeloid ecotropic viral integration site 1 homolog (mouse) 163 +/- 28 218 +/- 24 108 +/- 10 122 +/- 11 -0.000967 0.1680677 0.371723 0.4255201 0.7907528 METT10D methyltransferase 10 domain containing 191 +/- 20 242 +/- 24 146 +/- 12 174 +/- 17 -0.001511 0.1315229 0.219259 0.4735469 0.4689051 MFAP1 microfibrillar-associated protein 1 1476 +/- 22 1315 +/- 35 1367 +/- 41 1252 +/- 27 -0.012697 -0.004145 -0.047852 0.0023246 0.0629694 MGAT2 mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase 777 +/- 36 680 +/- 33 664 +/- 33 568 +/- 47 -0.006424 -0.073247 -0.135121 0.3775768 0.3160081 MGAT4A mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A 718 +/- 63 625 +/- 39 627 +/- 15 498 +/- 51 -0.021769 -0.242402 -0.064397 0.2104359 0.055879 MGC13379HSPC244 461 +/- 26 516 +/- 20 376 +/- 14 391 +/- 8 -9.49E-05 0.1287207 0.377785 0.0913592 0.7322016 MGC16372CG10958-like 283 +/- 27 218 +/- 22 163 +/- 14 194 +/- 34 -0.040009 -0.096923 0.434247 0.2029161 0.3777589 MGC16824esophageal cancer associated protein 765 +/- 47 860 +/- 57 683 +/- 33 683 +/- 31 -0.016935 0.2298522 0.988077 0.0974879 0.7414654 MGC17330HGFL gene 1002 +/- 83 1116 +/- 59 773 +/- 36 806 +/- 48 -0.000192 0.2917969 0.600396 0.5687043 0.5161542 MGC4172 short-chain dehydrogenase/reductase 870 +/- 42 789 +/- 23 813 +/- 49 709 +/- 40 -0.013958 -0.132823 -0.132952 0.2279703 0.4509919 MGST2 microsomal glutathione S-transferase 2 775 +/- 83 727 +/- 54 528 +/- 50 467 +/- 19 -0.000188 -0.63898 -0.293942 0.6565622 0.6880658 MICAL2 microtubule associated monoxygenase, calponin and LIM domain containing 2 3255 +/- 512 2565 +/- 156 2176 +/- 140 2192 +/- 134 -0.01536 -0.245748 0.933089 0.0991871 0.8330273 MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) 7449 +/- 286 6942 +/- 382 5425 +/- 93 5531 +/- 146 -5.35E-06 -0.314916 0.557763 0.2591961 0.8384949 MIF4GD MIF4G domain containing 691 +/- 34 723 +/- 49 622 +/- 29 586 +/- 34 -0.001584 0.6089853 -0.43925 0.2283763 0.9183326 mimitin Myc-induced mitochondria protein 964 +/- 50 955 +/- 68 819 +/- 53 831 +/- 32 -0.008033 -0.920226 0.852593 0.7613019 0.9948019 MIR16 membrane interacting protein of RGS16 117 +/- 15 140 +/- 23 72 +/- 9 98 +/- 13 -0.026688 0.4205332 0.138912 0.2024455 0.4829234 MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) 569 +/- 45 480 +/- 41 470 +/- 32 363 +/- 16 -0.005193 -0.174971 -0.019893 0.7383671 0.0561074 MNDA myeloid cell nuclear differentiation antigen 320 +/- 40 372 +/- 36 226 +/- 32 250 +/- 57 -0.008323 0.3491494 0.728875 0.1296748 0.9467625 MOBP myelin-associated oligodendrocyte basic protein 3329 +/- 750 6661 +/- 1634 1660 +/- 465 3347 +/- 458 -0.029555 0.106168 0.029747 0.0163244 0.0573451 MOG myelin oligodendrocyte glycoprotein 4168 +/- 662 5730 +/- 1129 2440 +/- 425 3331 +/- 467 -0.022341 0.2665493 0.193074 0.0701614 0.2461795 MON2 MON2 homolog (S. cerevisiae) 810 +/- 21 895 +/- 63 735 +/- 54 696 +/- 25 -0.009129 0.2463985 -0.532529 0.0622108 0.3918161 MORC4 MORC family CW-type zinc finger 4 242 +/- 15 260 +/- 30 191 +/- 15 194 +/- 13 -0.042593 0.6242526 0.850643 0.1401842 0.9475274 MOSPD2 motile sperm domain containing 2 378 +/- 37 537 +/- 58 304 +/- 24 339 +/- 26 -0.01698 0.0480053 0.356208 0.001366 0.4828524 MPDU1 mannose-P-dolichol utilization defect 1 988 +/- 28 918 +/- 26 917 +/- 34 890 +/- 35 -0.032858 -0.09339 -0.602937 0.0758011 0.3786318 MPDZ multiple PDZ domain protein 1604 +/- 47 1759 +/- 193 1514 +/- 42 1411 +/- 75 -0.045356 0.46568 -0.272578 0.1459382 0.399132 MPEG1 macrophage expressed gene 1 612 +/- 60 826 +/- 70 399 +/- 27 583 +/- 25 -0.001424 0.0432552 0.000738 0.0122355 0.0359659 MPPED1 metallophosphoesterase domain containing 1 1301 +/- 175 1135 +/- 79 413 +/- 60 397 +/- 75 -1.47E-05 -0.417146 -0.866829 0.2065673 0.7765558 M-RIP myosin phosphatase-Rho interacting protein 3617 +/- 157 3133 +/- 138 3127 +/- 109 2889 +/- 119 -0.019774 -0.043121 -0.177579 0.0014899 0.3564228 MRPL20 mitochondrial ribosomal protein L20 2020 +/- 172 2410 +/- 102 1610 +/- 52 1867 +/- 59 -0.000857 0.0863428 0.010571 0.1739134 0.0151383 MRPL22 mitochondrial ribosomal protein L22 706 +/- 20 652 +/- 26 641 +/- 17 556 +/- 21 -0.001229 -0.128265 -0.014089 0.2421444 0.2526271 MRPL28 mitochondrial ribosomal protein L28 661 +/- 39 642 +/- 27 526 +/- 24 554 +/- 24 -0.002908 -0.696383 0.443053 0.2747998 0.4539893 MRPL36 mitochondrial ribosomal protein L36 1273 +/- 70 1234 +/- 49 1013 +/- 33 1108 +/- 45 -0.003656 -0.661187 0.134003 0.9730805 0.2057185 MRPL37 mitochondrial ribosomal protein L37 3196 +/- 166 3187 +/- 166 2627 +/- 101 2901 +/- 86 -0.003272 -0.970737 0.06926 0.2821168 0.2189765 MRPL41 mitochondrial ribosomal protein L41 1938 +/- 120 1693 +/- 157 1481 +/- 38 1408 +/- 131 -0.000726 -0.245092 -0.616474 0.5279377 0.6660583

S2-genes differentially expressed across region.xls Page 56 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI MRPL51 mitochondrial ribosomal protein L51 1329 +/- 87 1194 +/- 51 1081 +/- 82 1052 +/- 32 -0.000206 -0.218219 -0.755498 0.3402625 0.8628368 MRPL53 mitochondrial ribosomal protein L53 2757 +/- 48 2752 +/- 123 1973 +/- 79 2085 +/- 134 -2.19E-06 -0.969825 0.49531 0.5118797 0.9462023 MRPL55 mitochondrial ribosomal protein L55 1219 +/- 64 1369 +/- 79 1087 +/- 53 1194 +/- 51 -0.022585 0.1695806 0.177039 0.60038 0.4932739 MRPS16 mitochondrial ribosomal protein S16 1490 +/- 80 1323 +/- 48 1292 +/- 51 1273 +/- 113 -0.041131 -0.109823 -0.880767 0.0446191 0.5076385 MRPS18A mitochondrial ribosomal protein S18A 633 +/- 57 659 +/- 22 556 +/- 25 527 +/- 16 -0.010532 0.6912774 -0.356718 0.9171512 0.887213 MRPS18B mitochondrial ribosomal protein S18B 1315 +/- 77 1289 +/- 70 1145 +/- 25 1180 +/- 45 -0.011096 -0.809417 0.525139 0.5904777 0.6973735 MRPS26 mitochondrial ribosomal protein S26 2291 +/- 233 2175 +/- 122 1963 +/- 161 1726 +/- 114 -0.002832 -0.67152 -0.260489 0.732496 0.1979787 MRPS27 mitochondrial ribosomal protein S27 1613 +/- 60 1618 +/- 99 1404 +/- 37 1535 +/- 77 -0.000628 0.9687653 0.178043 0.3215298 0.5521987 MRPS28 mitochondrial ribosomal protein S28 473 +/- 28 481 +/- 26 355 +/- 22 380 +/- 13 -0.000228 0.8415485 0.350608 0.9097605 0.9486482 MRPS33 mitochondrial ribosomal protein S33 2981 +/- 265 3013 +/- 172 2248 +/- 110 2590 +/- 162 -0.008836 0.9208983 0.12259 0.5991618 0.4506278 MRRF mitochondrial ribosome recycling factor 420 +/- 20 360 +/- 12 352 +/- 20 323 +/- 20 -0.019024 -0.032655 -0.331146 0.0505642 0.376247 MSRB2 methionine sulfoxide reductase B2 1907 +/- 249 1687 +/- 94 1639 +/- 202 1464 +/- 110 -0.002533 -0.43782 -0.47151 0.5855485 0.8323144 MST150 MSTP150 529 +/- 45 464 +/- 15 467 +/- 24 407 +/- 18 -0.014356 -0.218812 -0.076499 0.404919 0.0667702 MT1X metallothionein 1X 5805 +/- 490 4035 +/- 332 4142 +/- 463 3622 +/- 294 -0.003586 -0.015631 -0.370711 0.0501186 0.8429125 MTG1 mitochondrial GTPase 1 homolog (S. cerevisiae) 1704 +/- 61 1474 +/- 43 1403 +/- 55 1356 +/- 42 -0.00047 -0.01262 -0.51801 0.0267037 0.549807 MTHFD1L methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like 788 +/- 33 696 +/- 40 551 +/- 23 558 +/- 41 -0.000149 -0.107829 0.885582 0.0188315 0.8514756 MTIF2 mitochondrial translational initiation factor 2 1457 +/- 95 1709 +/- 108 1346 +/- 64 1482 +/- 33 -0.025055 0.1114125 0.098167 0.1213455 0.2730887 MTM1 myotubularin 1 350 +/- 14 409 +/- 32 325 +/- 20 353 +/- 7 -0.035518 0.1366969 0.248263 0.0559788 0.3139205 MTMR10 myotubularin related protein 10 2416 +/- 317 2968 +/- 315 1639 +/- 68 1599 +/- 104 -0.002336 0.2449853 -0.753464 0.0741602 0.1464852 MTO1 mitochondrial translation optimization 1 homolog (S. cerevisiae) 2273 +/- 147 2075 +/- 221 2012 +/- 80 2126 +/- 137 -0.030063 -0.476916 0.498153 0.2177795 0.9670432 MTRR 5-methyltetrahydrofolate-homocysteine methyltransferase reductase 1842 +/- 159 1672 +/- 213 1452 +/- 108 1499 +/- 180 -0.001705 -0.538157 0.831739 0.2474989 0.6712217 MTUS1 mitochondrial tumor suppressor 1 1480 +/- 263 2124 +/- 342 1103 +/- 78 1358 +/- 89 -0.031927 0.168812 0.060021 0.0389657 0.1914933 MXD1 MAX dimerization protein 1 648 +/- 28 589 +/- 27 530 +/- 21 515 +/- 29 -0.00672 -0.151254 -0.700638 0.0949698 0.9202084 MXI1 MAX interactor 1 6342 +/- 614 7312 +/- 652 4531 +/- 138 5071 +/- 166 -0.000504 0.3040709 0.036293 0.11012 0.0351373 MYD88 myeloid differentiation primary response gene (88) 210 +/- 31 284 +/- 16 174 +/- 17 212 +/- 13 -0.010976 0.0693878 0.107125 0.0648871 0.6070201 MYEOV2 myeloma overexpressed 2 11575 +/- 867 12859 +/- 629 9590 +/- 539 11808 +/- 395 -0.019511 0.2608522 0.009414 0.3886535 0.0341309 MYL6 myosin, light polypeptide 6, alkali, smooth muscle and non-muscle 5381 +/- 175 5499 +/- 158 4330 +/- 103 4685 +/- 116 -1.69E-05 0.6286577 0.048931 0.9036789 0.209868 MYL6B myosin, light polypeptide 6B, alkali, smooth muscle and non-muscle 3763 +/- 200 3536 +/- 211 2745 +/- 13 2729 +/- 79 -0.000354 -0.453449 -0.855573 0.3938505 0.7475447 MYLIP myosin regulatory light chain interacting protein 1437 +/- 163 1322 +/- 140 795 +/- 51 733 +/- 36 -0.000221 -0.604428 -0.34735 0.8347581 0.8685184 MYLK myosin, light polypeptide kinase 2425 +/- 309 3340 +/- 241 1446 +/- 132 2443 +/- 131 -0.000588 0.043083 0.000478 0.1166348 0.0693647 MYO15A myosin XVA 221 +/- 48 214 +/- 41 111 +/- 19 93 +/- 23 -0.000348 -0.903975 -0.564122 0.9883616 0.8142285 MYO1B myosin IB 240 +/- 40 180 +/- 30 189 +/- 25 121 +/- 13 -0.019778 -0.250919 -0.045613 0.4635443 0.0741961 MYO1D myosin ID 361 +/- 48 454 +/- 93 229 +/- 32 255 +/- 22 -0.008251 0.4045427 0.519761 0.1871262 0.2287992 MYO5A myosin VA (heavy polypeptide 12, myoxin) 9504 +/- 824 10302 +/- 557 8508 +/- 469 8961 +/- 216 -0.007725 0.4437191 0.409427 0.7794412 0.3378118 MYO9B myosin IXB 669 +/- 94 844 +/- 90 446 +/- 24 519 +/- 35 -0.001818 0.2050871 0.131864 0.1292527 0.2400304 NADSYN1 NAD synthetase 1 338 +/- 32 449 +/- 39 242 +/- 20 262 +/- 19 -0.000626 0.054237 0.498735 0.0355291 0.7809915 NAGK N-acetylglucosamine kinase 848 +/- 43 849 +/- 36 683 +/- 26 643 +/- 4 -0.000131 0.9812932 -0.188269 0.6721995 0.3228093 NAGPA N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase 1060 +/- 107 1025 +/- 54 924 +/- 50 826 +/- 63 -0.010452 -0.777136 -0.254905 0.3837368 0.2362316 NANS N-acetylneuraminic acid synthase (sialic acid synthase) 699 +/- 66 712 +/- 64 591 +/- 15 563 +/- 25 -0.040496 0.8932018 -0.368414 0.8032002 0.8005137 NAP1L1 nucleosome assembly protein 1-like 1 1463 +/- 33 1829 +/- 95 1400 +/- 79 1475 +/- 74 -0.038209 0.0102 0.499868 0.0008993 0.2561711 NAP5 Nck-associated protein 5 211 +/- 31 212 +/- 27 162 +/- 11 123 +/- 8 -0.011994 0.97362 -0.021145 0.6593897 0.1115944 NARF nuclear prelamin A recognition factor 560 +/- 39 505 +/- 31 498 +/- 33 455 +/- 17 -0.039852 -0.294264 -0.281931 0.1873588 0.3532489 NARS asparaginyl-tRNA synthetase 6379 +/- 455 5602 +/- 434 5379 +/- 327 5287 +/- 291 -0.016749 -0.244769 -0.839001 0.0345688 0.2870676 NAT12 N-acetyltransferase 12 1754 +/- 40 1710 +/- 10 1674 +/- 29 1650 +/- 39 -0.024339 -0.329531 -0.62656 0.3497704 0.5388663 NCAM1 neural cell adhesion molecule 1 374 +/- 80 1218 +/- 237 262 +/- 43 746 +/- 266 -0.031607 0.014441 0.142824 0.0932677 0.5633117

S2-genes differentially expressed across region.xls Page 57 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI NCBP2 nuclear cap binding protein subunit 2, 20kDa 5839 +/- 503 5405 +/- 196 4023 +/- 235 4471 +/- 238 -0.000535 -0.449293 0.212522 0.493286 0.0810031 NCKAP1L NCK-associated protein 1-like 212 +/- 13 256 +/- 26 173 +/- 11 197 +/- 12 -0.025597 0.1723018 0.19137 0.0092334 0.1482138 NDRG1 N-myc downstream regulated gene 1 6429 +/- 896 7904 +/- 978 4264 +/- 354 5467 +/- 340 -0.011262 0.2923671 0.036858 0.1415015 0.025735 NDST3 N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 409 +/- 45 412 +/- 44 278 +/- 41 254 +/- 28 -0.001514 0.9639593 -0.648075 0.8836409 0.9246601 NDST4 N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 338 +/- 57 277 +/- 56 197 +/- 42 121 +/- 18 -0.002682 -0.463618 -0.144953 0.5303195 0.2435026 NDUFA11 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 11, 14.7kDa 1903 +/- 101 1767 +/- 114 1531 +/- 70 1620 +/- 99 -0.007971 -0.395734 0.485658 0.0912642 0.8701878 NDUFA12 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 6412 +/- 235 6443 +/- 448 5561 +/- 145 5666 +/- 168 -0.003403 0.9531605 0.648569 0.8220009 0.7731163 NDUFA13 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 8687 +/- 338 8344 +/- 348 7000 +/- 229 7571 +/- 199 -0.000509 -0.496204 0.092413 0.2781405 0.0417498 NDUFA2 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa 5025 +/- 150 4531 +/- 197 4426 +/- 159 4245 +/- 198 -0.013638 -0.076187 -0.496555 0.2323814 0.2933485 NDUFA3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa 5433 +/- 483 5143 +/- 186 4163 +/- 321 4578 +/- 116 -0.013126 -0.594536 0.268405 0.58759 0.3382084 NDUFA4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa 16552 +/- 763 15922 +/- 532 13801 +/- 258 14573 +/- 339 -0.003205 -0.515137 0.10814 0.4871307 0.0164315 NDUFA6 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa 1534 +/- 35 1471 +/- 53 1392 +/- 49 1332 +/- 34 -0.002868 -0.349464 -0.340639 0.2262425 0.2648731 NDUFA7 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa 3451 +/- 140 3160 +/- 177 2509 +/- 49 2371 +/- 80 -2.53E-06 -0.227536 -0.186505 0.4181364 0.7692729 NDUFB11 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11, 17.3kDa 2865 +/- 96 2764 +/- 142 2466 +/- 71 2532 +/- 62 -0.000995 -0.567567 0.498781 0.2392619 0.6299135 NDUFB2 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa 2656 +/- 38 2528 +/- 107 2217 +/- 39 2426 +/- 38 -0.003763 -0.300337 0.003892 0.0102589 0.1145787 NDUFB3 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa 1376 +/- 67 1340 +/- 15 1233 +/- 19 1274 +/- 68 -0.044877 -0.624109 0.58888 0.7791277 0.8279813 NDUFB5 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa 5548 +/- 466 4759 +/- 317 4733 +/- 226 4438 +/- 426 -0.035636 -0.195699 -0.563035 0.0982054 0.160601 NDUFB6 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa 491 +/- 69 658 +/- 114 377 +/- 32 443 +/- 68 -0.01034 0.2446562 0.408038 0.029816 0.0384755 NDUFB8 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa 11769 +/- 658 13321 +/- 391 9711 +/- 250 12186 +/- 194 -0.00071 0.0763743 2.97E-05 0.311303 0.0182935 NDUFB9 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa 9059 +/- 465 9283 +/- 437 7901 +/- 152 8430 +/- 229 -0.004473 0.7333913 0.095287 0.9207544 0.1624003 NDUFC2 NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa 3438 +/- 105 3558 +/- 205 2851 +/- 67 3170 +/- 189 -1.17E-06 0.6167435 0.171232 0.6876896 0.5572069 NDUFS4 NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductas 4066 +/- 55 3938 +/- 133 3675 +/- 70 3676 +/- 75 -0.011756 -0.401939 0.994261 0.224438 0.5738107 NDUFV1 NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa 2923 +/- 155 2506 +/- 143 2595 +/- 65 2288 +/- 108 -0.051091 -0.076226 -0.045771 0.1271505 0.2553183 NECAP2 NECAP endocytosis associated 2 1224 +/- 129 1647 +/- 142 993 +/- 53 1204 +/- 15 -0.006308 0.0523344 0.009162 0.0413594 0.0504406 NEDD9 neural precursor cell expressed, developmentally down-regulated 9 171 +/- 24 264 +/- 31 142 +/- 23 201 +/- 20 -0.016703 0.0430915 0.08859 0.0470127 0.0403083 NEFH neurofilament, heavy polypeptide 200kDa 2571 +/- 416 2682 +/- 404 1722 +/- 165 1952 +/- 101 -0.018098 0.8512098 0.268276 0.8129419 0.298178 NEK3 NIMA (never in mitosis gene a)-related kinase 3 167 +/- 28 217 +/- 6 148 +/- 21 158 +/- 9 -0.002126 0.1358206 0.658683 0.0690078 0.6219159 NEK7 NIMA (never in mitosis gene a)-related kinase 7 2990 +/- 247 3754 +/- 214 2291 +/- 122 2919 +/- 48 -0.000365 0.0420786 0.002479 0.0245299 0.0114918 NENF neuron derived neurotrophic factor 194 +/- 26 226 +/- 18 170 +/- 12 166 +/- 16 -0.019878 0.3457196 -0.821354 0.1771557 0.9232304 NES nestin 445 +/- 71 622 +/- 83 293 +/- 42 359 +/- 34 -0.001403 0.1375265 0.25166 0.0984884 0.4228017 NET1 neuroepithelial cell transforming gene 1 818 +/- 40 947 +/- 56 737 +/- 38 793 +/- 20 -0.022346 0.0953697 0.238802 0.0424955 0.1892177 NETO2 neuropilin (NRP) and tolloid (TLL)-like 2 2261 +/- 64 1727 +/- 92 1797 +/- 68 1378 +/- 100 -1.76E-05 -0.001018 -0.009544 0.0059959 0.0224438 NFE2L2 nuclear factor (erythroid-derived 2)-like 2 2479 +/- 133 3346 +/- 356 1953 +/- 113 2015 +/- 87 -0.004502 0.0599949 0.671825 0.0028749 0.622519 NFE2L3 nuclear factor (erythroid-derived 2)-like 3 294 +/- 94 236 +/- 49 127 +/- 23 123 +/- 30 -0.044697 -0.598639 -0.920379 0.5087678 0.7486646 NFKBIZ Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta 167 +/- 11 264 +/- 26 150 +/- 6 206 +/- 14 -0.035425 0.0112849 0.01217 0.0001388 0.0254422 NGRN neugrin, neurite outgrowth associated 8356 +/- 531 7815 +/- 373 6974 +/- 247 6978 +/- 271 -0.019283 -0.426917 0.99351 0.3639453 0.9910679 NIBP NIK and IKK{beta} binding protein 744 +/- 67 889 +/- 46 598 +/- 42 755 +/- 42 -0.019114 0.1096693 0.026365 0.160192 0.1644102 NIFUN NifU-like N-terminal domain containing 10519 +/- 373 10858 +/- 390 9520 +/- 257 10727 +/- 229 -0.01697 0.5434208 0.00664 0.7958581 0.0285253 NINJ2 ninjurin 2 672 +/- 49 990 +/- 158 361 +/- 35 494 +/- 25 -0.002231 0.1042603 0.014367 0.0107827 0.03445 NIT1 nitrilase 1 692 +/- 54 611 +/- 14 607 +/- 33 558 +/- 26 -0.02054 -0.199232 -0.276493 0.2430751 0.2577545 NIT2 nitrilase family, member 2 1354 +/- 75 1495 +/- 47 1151 +/- 35 1286 +/- 75 -0.00068 0.1455497 0.155469 0.0787604 0.2018773 NKRF NF-kappaB repressing factor 1178 +/- 77 1360 +/- 69 1124 +/- 61 1084 +/- 52 -0.019362 0.1076942 -0.62943 0.0731064 0.9080355 NKX2-2 NK2 transcription factor related, locus 2 (Drosophila) 1239 +/- 183 1408 +/- 216 872 +/- 56 902 +/- 35 -0.01107 0.5666356 0.668881 0.3541144 0.3494846 NME2 non-metastatic cells 2, protein (NM23B) expressed in 9915 +/- 392 9618 +/- 216 8873 +/- 225 9524 +/- 210 -0.040218 -0.526307 0.063238 0.3844042 0.1183607 NME3 non-metastatic cells 3, protein expressed in 1224 +/- 85 1346 +/- 148 1100 +/- 107 1153 +/- 204 -0.004854 0.4960717 0.8285 0.9106562 0.5549015

S2-genes differentially expressed across region.xls Page 58 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI NMI N-myc (and STAT) interactor 480 +/- 56 429 +/- 54 345 +/- 21 313 +/- 43 -0.01103 -0.524033 -0.530292 0.6894528 0.985168 NMRAL1 NmrA-like family domain containing 1 1470 +/- 75 1003 +/- 79 1214 +/- 54 797 +/- 29 -0.000141 -0.00164 -0.000186 0.0303439 0.0085358 NOLA1 nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) 834 +/- 97 901 +/- 60 761 +/- 55 717 +/- 46 -0.044875 0.5685022 -0.552782 0.3386188 0.9459478 NOPE likely ortholog of mouse neighbor of Punc E11 1990 +/- 160 1901 +/- 105 1805 +/- 130 1684 +/- 49 -0.047746 -0.655182 -0.413241 0.9018591 0.7427891 NOSTRIN nitric oxide synthase trafficker 107 +/- 8 126 +/- 9 91 +/- 8 86 +/- 4 -0.020274 0.1428083 -0.632002 0.0395262 0.4937208 NPAT nuclear protein, ataxia-telangiectasia locus 490 +/- 15 619 +/- 32 435 +/- 15 535 +/- 41 -0.001088 0.0079427 0.071889 0.0227281 0.1146014 NPC1 Niemann-Pick disease, type C1 1631 +/- 195 2450 +/- 429 909 +/- 54 1244 +/- 72 -0.003597 0.1255651 0.006001 0.0150477 0.004993 NPC2 Niemann-Pick disease, type C2 3019 +/- 294 3544 +/- 283 2140 +/- 161 2387 +/- 146 -0.001404 0.2267512 0.284005 0.1434239 0.6376006 NR4A1 nuclear receptor subfamily 4, group A, member 1 363 +/- 65 247 +/- 46 154 +/- 38 115 +/- 20 -8.74E-05 -0.180247 -0.38616 0.1178416 0.6747038 NR4A2 nuclear receptor subfamily 4, group A, member 2 769 +/- 158 560 +/- 84 472 +/- 79 451 +/- 48 -0.047099 -0.278426 -0.826346 0.4680283 0.8414883 NRF1 nuclear respiratory factor 1 180 +/- 11 223 +/- 12 164 +/- 5 186 +/- 16 -0.006482 0.0251685 0.246261 0.0249377 0.1096532 NRIP1 nuclear receptor interacting protein 1 2778 +/- 469 2228 +/- 121 1966 +/- 107 1869 +/- 103 -0.038244 -0.301729 -0.529434 0.2950315 0.7692809 NSD1 nuclear receptor binding SET domain protein 1 901 +/- 32 858 +/- 45 828 +/- 18 773 +/- 42 -0.027473 -0.462006 -0.278625 0.6850018 0.6727283 NSMCE1 non-SMC element 1 homolog (S. cerevisiae) 1331 +/- 123 1415 +/- 177 876 +/- 51 895 +/- 122 -0.00015 0.7033895 0.888826 0.9469581 0.5854921 NT5C3L 5'-nucleotidase, cytosolic III-like 1161 +/- 46 1096 +/- 101 948 +/- 60 945 +/- 59 -0.005893 -0.579072 -0.970947 0.0620936 0.5538538 NTHL1 nth endonuclease III-like 1 (E. coli) 389 +/- 30 390 +/- 16 334 +/- 23 333 +/- 14 -0.013727 0.9681059 -0.996248 0.6648193 0.8282208 NUAK1 NUAK family, SNF1-like kinase, 1 3806 +/- 657 4343 +/- 436 2886 +/- 286 2918 +/- 140 -0.018164 0.5135212 0.921418 0.4925862 0.5538532 NUBP1 nucleotide binding protein 1 (MinD homolog, E. coli) 679 +/- 25 548 +/- 12 607 +/- 21 506 +/- 19 -0.004407 -0.002201 -0.006862 0.0254274 0.0773208 NUCB1 nucleobindin 1 1411 +/- 89 1393 +/- 56 1288 +/- 74 1371 +/- 50 -0.015575 -0.872065 0.378212 0.5716614 0.5769925 NUDT1 nudix (nucleoside diphosphate linked moiety X)-type motif 1 660 +/- 103 577 +/- 62 543 +/- 105 480 +/- 75 -0.01441 -0.511029 -0.636428 0.5019372 0.4903507 NUDT14 nudix (nucleoside diphosphate linked moiety X)-type motif 14 619 +/- 49 467 +/- 78 535 +/- 52 434 +/- 91 -0.018479 -0.133543 -0.367287 0.051609 0.1362729 NUDT16L1nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 1200 +/- 60 1085 +/- 44 949 +/- 40 960 +/- 27 -0.001393 -0.154984 0.820666 0.2076926 0.6732427 NUDT5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 884 +/- 75 1105 +/- 67 672 +/- 11 954 +/- 63 -0.003564 0.0533906 0.010161 0.0942105 0.1224147 NUDT9P1 nudix (nucleoside diphosphate linked moiety X)-type motif 9 pseudogene 1 172 +/- 13 232 +/- 27 116 +/- 12 148 +/- 23 -0.003603 0.0846632 0.270128 0.1281903 0.3168278 NUP205 nucleoporin 205kDa 445 +/- 17 522 +/- 39 400 +/- 9 414 +/- 19 -0.01357 0.1141584 0.503183 0.1107064 0.2926634 NUP98 nucleoporin 98kDa 569 +/- 31 670 +/- 43 499 +/- 29 570 +/- 13 -0.012462 0.0905121 0.060159 0.0900318 0.0618543 NXPH3 neurexophilin 3 182 +/- 77 246 +/- 74 68 +/- 14 43 +/- 11 -0.009881 0.5556689 -0.201123 0.7609458 0.3045937 NYREN18 NEDD8 ultimate buster-1 1202 +/- 131 1359 +/- 62 1083 +/- 43 1143 +/- 33 -0.049319 0.3153611 0.294697 0.1584412 0.0800793 OACT2 O-acyltransferase (membrane bound) domain containing 2 2382 +/- 161 2359 +/- 217 1902 +/- 68 1895 +/- 91 -0.00383 -0.933936 -0.950505 0.6345297 0.7856182 OAT ornithine aminotransferase (gyrate atrophy) 7034 +/- 153 7241 +/- 109 6462 +/- 50 6686 +/- 368 -0.002156 0.2995915 0.577277 0.5914579 0.7549995 OBFC1 oligonucleotide/oligosaccharide-binding fold containing 1 224 +/- 12 220 +/- 22 169 +/- 11 211 +/- 12 -0.012381 -0.891075 0.028949 0.4253951 0.3264349 OCA2 oculocutaneous albinism II (pink-eye dilution homolog, mouse) 224 +/- 39 164 +/- 22 175 +/- 18 148 +/- 22 -0.033424 -0.21941 -0.373784 0.1253069 0.1681369 OCIAD2 OCIA domain containing 2 1106 +/- 94 912 +/- 89 801 +/- 93 910 +/- 93 -0.045137 -0.165999 0.430209 0.0451204 0.6888983 OFD1 oral-facial-digital syndrome 1 747 +/- 52 782 +/- 43 690 +/- 41 664 +/- 24 -0.009098 0.6165589 -0.5857 0.362779 0.9056248 OGDH oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1659 +/- 60 1455 +/- 67 1434 +/- 34 1400 +/- 37 -0.010026 -0.045988 -0.523984 0.0404441 0.3839513 OLFM3 olfactomedin 3 567 +/- 99 458 +/- 41 221 +/- 45 166 +/- 25 -0.000122 -0.343362 -0.319771 0.2686437 0.3295441 OLFML2A olfactomedin-like 2A 225 +/- 17 206 +/- 13 153 +/- 12 173 +/- 12 -0.006244 -0.390797 0.261728 0.4884851 0.676613 OLFML2B olfactomedin-like 2B 198 +/- 18 198 +/- 17 152 +/- 6 158 +/- 9 -0.006676 0.9872658 0.565581 0.6675167 0.3278659 OLFML3 olfactomedin-like 3 953 +/- 74 970 +/- 68 745 +/- 45 686 +/- 18 -0.000902 0.8654499 -0.263746 0.5878086 0.3127015 OLIG1 oligodendrocyte transcription factor 1 10505 +/- 1321 11917 +/- 1508 6389 +/- 320 7664 +/- 455 -0.002276 0.4974144 0.053366 0.2657782 0.0402461 OPLAH 5-oxoprolinase (ATP-hydrolysing) 727 +/- 84 771 +/- 88 617 +/- 45 553 +/- 51 -0.010416 0.7250965 -0.37351 0.5829988 0.4664037 OPRK1 opioid receptor, kappa 1 246 +/- 37 227 +/- 34 78 +/- 8 108 +/- 13 -0.000554 -0.710685 0.100116 0.3842987 0.2003617 ORMDL2 ORM1-like 2 (S. cerevisiae) 365 +/- 22 344 +/- 25 270 +/- 9 290 +/- 26 -0.001703 -0.552489 0.5135 0.7526918 0.8789357 OSBPL11 oxysterol binding protein-like 11 1537 +/- 69 1374 +/- 41 1376 +/- 35 1266 +/- 31 -0.011451 -0.075652 -0.044285 0.0694511 0.0146336 OSBPL1A oxysterol binding protein-like 1A 5228 +/- 458 5797 +/- 389 3578 +/- 80 4173 +/- 148 -0.000969 0.3665203 0.011533 0.3098806 0.0059963

S2-genes differentially expressed across region.xls Page 59 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI OSBPL3 oxysterol binding protein-like 3 336 +/- 79 263 +/- 48 202 +/- 35 165 +/- 14 -0.004859 -0.453522 -0.368408 0.5264513 0.3601188 OSBPL9 oxysterol binding protein-like 9 1350 +/- 86 1500 +/- 155 981 +/- 36 1019 +/- 37 -0.000603 0.4223128 0.482898 0.3558818 0.6831957 OSGEP O-sialoglycoprotein endopeptidase 459 +/- 30 536 +/- 32 410 +/- 14 486 +/- 23 -0.0199 0.11213 0.027453 0.4425687 0.0759765 OSTF1 osteoclast stimulating factor 1 992 +/- 75 1259 +/- 157 723 +/- 75 891 +/- 79 -0.008483 0.1667636 0.158565 0.0311753 0.3869694 OVOL2 ovo-like 2 (Drosophila) 222 +/- 74 242 +/- 63 89 +/- 11 109 +/- 27 -0.003771 0.8452813 0.522729 0.9832937 0.655419 OXA1L oxidase (cytochrome c) assembly 1-like 1415 +/- 12 1313 +/- 88 1244 +/- 45 1227 +/- 46 -0.002782 -0.302886 -0.800921 0.1264329 0.8381491 OXR1 oxidation resistance 1 6372 +/- 469 5445 +/- 539 4933 +/- 406 3985 +/- 371 -0.002007 -0.223875 -0.118585 0.148656 0.2086449 OXSM 3-oxoacyl-ACP synthase, mitochondrial 285 +/- 30 283 +/- 19 233 +/- 24 248 +/- 15 -0.018663 -0.970132 0.625145 0.5006826 0.7032857 P2RY12 purinergic receptor P2Y, G-protein coupled, 12 1248 +/- 88 1177 +/- 79 1077 +/- 48 848 +/- 66 -0.011588 -0.565026 -0.023701 0.9972657 0.0388881 P8 p8 protein (candidate of metastasis 1) 397 +/- 62 578 +/- 55 378 +/- 56 493 +/- 36 -0.036934 0.0539708 0.12096 0.0367176 0.1038003 PACRG PARK2 co-regulated 1071 +/- 37 1394 +/- 73 893 +/- 44 1132 +/- 74 -0.000382 0.0049231 0.028089 0.0005899 0.0063267 PACSIN3 protein kinase C and casein kinase substrate in neurons 3 165 +/- 24 204 +/- 22 134 +/- 13 146 +/- 19 -0.042943 0.2563319 0.605792 0.3097612 0.4678509 PAIP2 poly(A) binding protein interacting protein 2 6014 +/- 283 6884 +/- 216 5284 +/- 145 5886 +/- 240 -0.001414 0.0363882 0.070781 0.0951697 0.28479 PAM peptidylglycine alpha-amidating monooxygenase 9384 +/- 821 7664 +/- 510 6095 +/- 300 5478 +/- 570 -0.000307 -0.111477 -0.374081 0.0420264 0.7853194 PAOX polyamine oxidase (exo-N4-amino) 424 +/- 48 456 +/- 37 383 +/- 30 376 +/- 20 -0.035244 0.6145202 -0.847774 0.7240013 0.5500314 PAPSS2 3'-phosphoadenosine 5'-phosphosulfate synthase 2 312 +/- 32 396 +/- 30 220 +/- 17 217 +/- 10 -0.000418 0.0823627 -0.874406 0.1284375 0.4088238 PARP12 poly (ADP-ribose) polymerase family, member 12 112 +/- 11 143 +/- 10 92 +/- 10 84 +/- 8 -0.002556 0.0689912 -0.54295 0.0237215 0.4426224 PARP14 poly (ADP-ribose) polymerase family, member 14 113 +/- 11 138 +/- 13 97 +/- 7 109 +/- 7 -0.014107 0.1627839 0.227367 0.5921469 0.707575 PBX1 Pre-B-cell leukemia transcription factor 1 1716 +/- 118 2769 +/- 217 1455 +/- 111 2238 +/- 91 -0.003412 0.0029746 0.000413 0.0044114 0.0061288 PCAF p300/CBP-associated factor 2476 +/- 204 3098 +/- 223 2140 +/- 50 2124 +/- 43 -0.00566 0.0667669 -0.819142 0.0062563 0.9549231 PCDH10 protocadherin 10 956 +/- 148 1051 +/- 135 653 +/- 82 380 +/- 31 -0.000867 0.6474616 -0.019473 0.7187845 0.0700467 PCDHA9 protocadherin alpha 9 3547 +/- 76 3268 +/- 189 3072 +/- 119 3035 +/- 114 -0.036055 -0.2163 -0.827638 0.0443213 0.679443 PCDHGB6 protocadherin gamma subfamily B, 6 4504 +/- 348 5671 +/- 569 2978 +/- 131 3473 +/- 103 -0.000517 0.1168144 0.016108 0.0748677 0.0446749 PCID2 PCI domain containing 2 422 +/- 26 371 +/- 24 406 +/- 11 289 +/- 11 -0.037605 -0.183262 -4.09E-05 0.0833054 0.0063696 PCNP PEST proteolytic signal containing nuclear protein 4478 +/- 358 5960 +/- 334 4068 +/- 199 4741 +/- 246 -0.010461 0.0127892 0.065491 0.0062998 0.2290232 PCP4L1 Purkinje cell protein 4 like 1 1327 +/- 152 1335 +/- 258 726 +/- 175 514 +/- 83 -0.000481 0.979951 -0.309747 0.5903336 0.6198512 PCSK1 proprotein convertase subtilisin/kexin type 1 2563 +/- 313 2492 +/- 184 1441 +/- 236 1764 +/- 250 -0.002163 -0.850131 0.372176 0.8146939 0.4633285 PCSK2 proprotein convertase subtilisin/kexin type 2 3146 +/- 220 2328 +/- 198 2601 +/- 119 2049 +/- 105 -0.013546 -0.020418 -0.006951 0.0123245 0.060491 PDCD10 programmed cell death 10 2306 +/- 97 2498 +/- 84 2142 +/- 43 2106 +/- 115 -0.024685 0.1668987 -0.782766 0.1896822 0.0991738 PDCD11 programmed cell death 11 478 +/- 26 501 +/- 23 398 +/- 17 429 +/- 19 -3.85E-05 0.513203 0.250095 0.714708 0.7772868 PDCD8 programmed cell death 8 (apoptosis-inducing factor) 568 +/- 20 580 +/- 30 482 +/- 21 531 +/- 26 -0.032765 0.7374145 0.184882 0.8803758 0.6851928 PDE4B phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila 146 +/- 33 186 +/- 30 95 +/- 9 117 +/- 10 -0.007024 0.3911785 0.137745 0.1904672 0.0449681 PDE8A Phosphodiesterase 8A 844 +/- 229 1285 +/- 192 545 +/- 66 690 +/- 45 -0.010516 0.1714711 0.103609 0.0417375 0.0495444 PDE9A phosphodiesterase 9A 625 +/- 72 588 +/- 32 503 +/- 39 431 +/- 16 -0.008911 -0.646916 -0.136198 0.5876266 0.4216819 PDF peptide deformylase (mitochondrial) 1600 +/- 78 1639 +/- 99 1424 +/- 100 1346 +/- 26 -0.015368 0.7644762 -0.479925 0.9176751 0.8806535 PDHA1 pyruvate dehydrogenase (lipoamide) alpha 1 2108 +/- 81 2051 +/- 151 1831 +/- 52 1761 +/- 167 -0.016965 -0.746885 -0.707375 0.1758669 0.2540294 PDLIM2 PDZ and LIM domain 2 (mystique) 370 +/- 20 395 +/- 31 313 +/- 4 310 +/- 25 -0.001546 0.5144343 -0.893563 0.5010098 0.4315887 PDRG1 p53 and DNA damage regulated 1 1527 +/- 55 1634 +/- 56 1412 +/- 56 1486 +/- 25 -0.000572 0.2006981 0.267419 0.2814875 0.3144108 PDZD11 PDZ domain containing 11 2816 +/- 76 2619 +/- 92 2358 +/- 67 2227 +/- 79 -0.000108 -0.131682 -0.24046 0.1497944 0.3299025 PDZRN4 PDZ domain containing RING finger 4 961 +/- 82 934 +/- 58 831 +/- 30 732 +/- 22 -0.021366 -0.790399 -0.028345 0.5257061 0.0958787 PEBP4 phosphatidylethanolamine-binding protein 4 169 +/- 29 121 +/- 16 68 +/- 11 89 +/- 16 -0.005699 -0.190034 0.29898 0.0347136 0.4226273 PECAM1 Platelet/endothelial cell adhesion molecule (CD31 antigen) 362 +/- 44 381 +/- 13 279 +/- 26 306 +/- 20 -0.016358 0.7042854 0.439124 0.6837403 0.6043239 PECI peroxisomal D3,D2-enoyl-CoA isomerase 1599 +/- 178 1627 +/- 109 1420 +/- 126 1470 +/- 74 -0.028385 0.8957688 0.740328 0.6469807 0.7405449 PEX16 peroxisomal biogenesis factor 16 681 +/- 46 699 +/- 17 620 +/- 21 649 +/- 36 -0.031751 0.7290861 0.517346 0.8018593 0.5021918 PFDN1 prefoldin subunit 1 3757 +/- 69 3495 +/- 74 3517 +/- 72 3281 +/- 42 -0.02002 -0.026758 -0.022375 0.003462 0.1034135

S2-genes differentially expressed across region.xls Page 60 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI PFDN2 prefoldin subunit 2 2401 +/- 84 2442 +/- 73 1814 +/- 33 1910 +/- 40 -9.22E-07 0.718541 0.101826 0.5324639 0.0593668 PFDN5 prefoldin subunit 5 3533 +/- 418 4256 +/- 249 3196 +/- 420 3430 +/- 126 -0.015965 0.1746728 0.613775 0.2705479 0.9476383 PFDN6 prefoldin subunit 6 2309 +/- 119 2019 +/- 59 1875 +/- 44 1786 +/- 69 -0.002889 -0.06373 -0.311792 0.1052694 0.968735 PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 531 +/- 38 759 +/- 35 440 +/- 25 533 +/- 18 -0.00079 0.0013335 0.014641 0.005562 0.0135006 PGAM1 phosphoglycerate mutase 1 (brain) 11785 +/- 417 13568 +/- 474 10224 +/- 320 12402 +/- 471 -0.001479 0.0183042 0.006018 0.1469321 0.0390915 PGAP1 GPI deacylase 4359 +/- 249 3952 +/- 304 3230 +/- 165 2950 +/- 203 -0.000126 -0.325559 -0.315204 0.2164275 0.3399198 PGRMC2 progesterone receptor membrane component 2 1541 +/- 44 1588 +/- 101 1223 +/- 49 1264 +/- 53 -0.000474 0.6787798 0.581483 0.7613088 0.9359732 PHACTR3 phosphatase and actin regulator 3 1160 +/- 77 1317 +/- 94 980 +/- 47 1072 +/- 52 -0.017441 0.2261748 0.224925 0.147136 0.3561551 PHB prohibitin 649 +/- 61 637 +/- 42 571 +/- 12 542 +/- 22 -0.034471 -0.871404 -0.30262 0.8334379 0.0325261 PHC1 polyhomeotic-like 1 (Drosophila) 853 +/- 33 913 +/- 40 797 +/- 21 763 +/- 22 -0.013407 0.2725264 -0.297689 0.3228185 0.3129029 PHCA phytoceramidase, alkaline 897 +/- 115 1163 +/- 106 725 +/- 49 780 +/- 36 -0.011577 0.119908 0.39205 0.0552162 0.570056 PHF13 PHD finger protein 13 758 +/- 20 767 +/- 20 684 +/- 16 705 +/- 22 -0.000285 0.7433085 0.458934 0.6379738 0.3248581 PHGDH phosphoglycerate dehydrogenase 2919 +/- 250 3311 +/- 176 2445 +/- 152 2384 +/- 122 -0.001847 0.231559 -0.761753 0.1052867 0.5845992 PHKA1 Phosphorylase kinase, alpha 1 (muscle) 995 +/- 57 1071 +/- 4 849 +/- 59 924 +/- 42 -0.01196 0.2419015 0.327334 0.5001502 0.6329023 PHLDA3 pleckstrin homology-like domain, family A, member 3 372 +/- 32 407 +/- 35 321 +/- 16 317 +/- 27 -0.005693 0.4766902 -0.893488 0.2156886 0.6595865 PHLDB1 pleckstrin homology-like domain, family B, member 1 246 +/- 32 207 +/- 20 159 +/- 23 156 +/- 37 -0.039867 -0.339027 -0.963218 0.616177 0.5000766 PHPT1 phosphohistidine phosphatase 1 2789 +/- 69 3031 +/- 99 2586 +/- 107 2786 +/- 69 -0.002696 0.0753768 0.15298 0.208543 0.6048488 PHYHIPL phytanoyl-CoA 2-hydroxylase interacting protein-like 4278 +/- 428 4666 +/- 474 3197 +/- 254 3741 +/- 221 -0.00608 0.5572128 0.140476 0.7375028 0.4988666 PI4K2B phosphatidylinositol 4-kinase type 2 beta 120 +/- 13 146 +/- 13 102 +/- 4 116 +/- 8 -0.006752 0.1855562 0.171197 0.0602943 0.1328526 PIAS3 protein inhibitor of activated STAT, 3 677 +/- 60 752 +/- 37 547 +/- 51 545 +/- 31 -0.005598 0.3124513 -0.983924 0.2760691 0.9400962 PIAS4 protein inhibitor of activated STAT, 4 424 +/- 32 442 +/- 27 370 +/- 26 349 +/- 39 -0.003322 0.6808359 -0.67535 0.5078991 0.9813771 PICK1 protein interacting with PRKCA 1 578 +/- 46 694 +/- 80 480 +/- 26 562 +/- 28 -0.023275 0.2439404 0.063743 0.3895662 0.2224386 PIGF phosphatidylinositol glycan, class F 1304 +/- 20 1281 +/- 57 1162 +/- 26 1211 +/- 43 -0.025344 -0.718521 0.364852 0.7980427 0.8485982 PIK3AP1 phosphoinositide-3-kinase adaptor protein 1 176 +/- 27 213 +/- 17 122 +/- 10 136 +/- 16 -0.004491 0.2772138 0.508031 0.3272904 0.7934704 PIK3C2A Phosphoinositide-3-kinase, class 2, alpha polypeptide 1130 +/- 44 1263 +/- 40 1085 +/- 33 1109 +/- 30 -0.003738 0.0481159 0.603829 0.0485674 0.7559619 PIK3C2B phosphoinositide-3-kinase, class 2, beta polypeptide 885 +/- 57 911 +/- 93 569 +/- 31 622 +/- 33 -0.000412 0.8193608 0.270407 0.3787876 0.0631785 PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha) 2940 +/- 204 3517 +/- 224 2478 +/- 130 2184 +/- 153 -0.000846 0.0865263 -0.179755 0.0258247 0.4474323 PIK3R3 phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) 618 +/- 41 536 +/- 20 397 +/- 29 423 +/- 20 -0.000455 -0.112294 0.481118 0.049276 0.5690352 PIK4CB phosphatidylinositol 4-kinase, catalytic, beta polypeptide 1465 +/- 48 1509 +/- 75 1268 +/- 27 1411 +/- 57 -0.033406 0.6297321 0.067029 0.2652995 0.4574089 PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type I, beta 1195 +/- 134 787 +/- 70 608 +/- 59 541 +/- 13 -0.001939 -0.028873 -0.314437 0.0181839 0.2623352 PIP5K3 phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III 1685 +/- 66 1442 +/- 57 1387 +/- 56 1232 +/- 71 -0.000187 -0.020366 -0.126116 0.1793597 0.222708 PITPNA phosphatidylinositol transfer protein, alpha 1944 +/- 110 1824 +/- 34 1554 +/- 72 1700 +/- 62 -0.009003 -0.336495 0.157894 0.2482591 0.3897361 PITPNB phosphatidylinositol transfer protein, beta 4243 +/- 114 4008 +/- 152 3325 +/- 75 3545 +/- 134 -0.000116 -0.246936 0.199195 0.0155593 0.4378877 PITRM1 Pitrilysin metallopeptidase 1 1006 +/- 168 1405 +/- 240 721 +/- 69 801 +/- 48 -0.010961 0.2066023 0.364492 0.0254679 0.115179 PKN1 protein kinase N1 1025 +/- 87 1327 +/- 101 952 +/- 42 1034 +/- 60 -0.051585 0.0479771 0.299131 0.1114371 0.5076743 PLAC8 placenta-specific 8 299 +/- 25 311 +/- 73 160 +/- 9 212 +/- 42 -0.008282 0.8776749 0.281755 0.2507321 0.1082357 PLAC9 placenta-specific 9 1835 +/- 282 1130 +/- 254 1524 +/- 212 916 +/- 172 -0.023748 -0.093037 -0.053552 0.4320796 0.3136659 PLCH1 phospholipase C, eta 1 327 +/- 12 405 +/- 32 217 +/- 23 304 +/- 19 -3.84E-05 0.0581358 0.016981 0.1075869 0.099588 PLCXD3 phosphatidylinositol-specific phospholipase C, X domain containing 3 1405 +/- 285 1244 +/- 206 979 +/- 108 964 +/- 202 -0.026609 -0.656499 -0.950889 0.4886636 0.3441277 PLD1 phospholipase D1, phosphatidylcholine-specific 225 +/- 35 314 +/- 48 141 +/- 17 181 +/- 14 -0.008504 0.1694986 0.103033 0.0679803 0.400129 PLEKHA7 pleckstrin homology domain containing, family A member 7 234 +/- 8 229 +/- 17 183 +/- 6 141 +/- 17 -0.00092 -0.774243 -0.072415 0.9105697 0.0003622 PLEKHB1 pleckstrin homology domain containing, family B (evectins) member 1 6842 +/- 885 9643 +/- 1345 4705 +/- 239 6453 +/- 287 -0.01047 0.1174381 0.001452 0.0255133 0.0059289 PLEKHF1 pleckstrin homology domain containing, family F (with FYVE domain) member 1 337 +/- 32 429 +/- 38 283 +/- 22 270 +/- 18 -0.003912 0.0953419 -0.667729 0.0669687 0.9679047 PLEKHG1 pleckstrin homology domain containing, family G (with RhoGef domain) member 1 324 +/- 38 374 +/- 35 261 +/- 21 300 +/- 31 -0.01809 0.3621849 0.328418 0.9659273 0.9676069 PLEKHH1 pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 5498 +/- 891 7056 +/- 1244 2329 +/- 184 3044 +/- 221 -0.001295 0.3350938 0.037095 0.1269824 0.055862

S2-genes differentially expressed across region.xls Page 61 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI PLEKHH2 pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 148 +/- 20 195 +/- 19 84 +/- 7 103 +/- 7 -0.000229 0.1251707 0.093024 0.0298257 0.0670013 PLEKHM2 pleckstrin homology domain containing, family M (with RUN domain) member 2 1209 +/- 132 1445 +/- 88 1020 +/- 44 987 +/- 49 -0.005401 0.172739 -0.630274 0.2787292 0.9036257 PLK1 polo-like kinase 1 (Drosophila) 21403 +/- 877 23680 +/- 475 19863 +/- 1093 22695 +/- 710 -0.047931 0.0530814 0.060486 0.3501264 0.3189812 PLK4 polo-like kinase 4 (Drosophila) 159 +/- 9 169 +/- 20 141 +/- 15 123 +/- 14 -0.021462 0.667746 -0.413566 0.5529981 0.4850564 PLLP plasma membrane proteolipid (plasmolipin) 5475 +/- 1056 7693 +/- 1468 2458 +/- 331 3460 +/- 405 -0.004559 0.2510121 0.09102 0.0579541 0.0697138 PLS3 plastin 3 (T isoform) 3006 +/- 316 3435 +/- 348 1917 +/- 119 2025 +/- 109 -0.001069 0.3829981 0.521281 0.1263417 0.4713675 PLXNB3 plexin B3 457 +/- 56 453 +/- 43 384 +/- 19 316 +/- 15 -0.015855 -0.953366 -0.019454 0.6546227 0.0750267 PLXNC1 plexin C1 1648 +/- 174 1573 +/- 93 1087 +/- 85 1061 +/- 49 -2.66E-05 -0.713422 -0.803315 0.7716299 0.668172 PMM2 phosphomannomutase 2 886 +/- 41 829 +/- 26 670 +/- 33 687 +/- 24 -0.000342 -0.277974 0.694491 0.1618448 0.9125394 PMP2 peripheral myelin protein 2 1246 +/- 290 2589 +/- 684 671 +/- 122 1112 +/- 259 -0.000848 0.1153266 0.176269 0.2347837 0.7818775 PMP22 Peripheral myelin protein 22 414 +/- 67 541 +/- 45 286 +/- 24 316 +/- 42 -0.00316 0.1519767 0.563274 0.1115839 0.9803347 PMPCB peptidase (mitochondrial processing) beta 2214 +/- 95 2048 +/- 139 1994 +/- 49 1865 +/- 81 -0.017456 -0.351055 -0.213294 0.4222111 0.3842492 PMVK phosphomevalonate kinase 1142 +/- 76 1074 +/- 69 930 +/- 42 963 +/- 63 -0.012257 -0.517031 0.681947 0.2293754 0.9979378 PNKD paroxysmal nonkinesiogenic dyskinesia 1868 +/- 146 1953 +/- 60 1610 +/- 94 1532 +/- 64 -0.013647 0.6093361 -0.507682 0.9564998 0.4677922 PNMA3 paraneoplastic antigen MA3 683 +/- 71 695 +/- 53 532 +/- 44 566 +/- 30 -0.002264 0.8926914 0.542634 0.6848308 0.4307017 PNOC prepronociceptin 565 +/- 80 656 +/- 158 457 +/- 42 393 +/- 33 -0.036304 0.6197071 -0.260369 0.7072795 0.4112155 POLA polymerase (DNA directed), alpha 420 +/- 30 480 +/- 34 376 +/- 13 382 +/- 11 -0.008573 0.2145296 0.736805 0.045781 0.4126258 POLE4 polymerase (DNA-directed), epsilon 4 (p12 subunit) 2611 +/- 103 2205 +/- 69 2073 +/- 57 2027 +/- 37 -0.004837 -0.009911 -0.52405 0.0030335 0.945593 POLR2G polymerase (RNA) II (DNA directed) polypeptide G 1501 +/- 46 1883 +/- 158 1045 +/- 54 1288 +/- 36 -0.000348 0.0603422 0.005367 0.0213566 0.0389687 POLR2I polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa 4116 +/- 295 4115 +/- 205 3411 +/- 135 3674 +/- 92 -0.001498 -0.998633 0.143612 0.883904 0.1430231 POLR2L polymerase (RNA) II (DNA directed) polypeptide L, 7.6kDa 2034 +/- 162 1876 +/- 118 1448 +/- 68 1615 +/- 80 -0.003944 -0.453163 0.150771 0.5519555 0.1040439 POMGNT1 protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase 1662 +/- 85 1667 +/- 87 1300 +/- 31 1268 +/- 36 -2.09E-05 0.9681029 -0.516174 0.5687295 0.7351611 POP5 processing of precursor 5, ribonuclease P/MRP subunit (S. cerevisiae) 1675 +/- 44 1502 +/- 87 1357 +/- 28 1271 +/- 48 -0.000971 -0.116937 -0.175332 0.0366651 0.1180031 POU3F1 POU domain, class 3, transcription factor 1 5136 +/- 1055 4008 +/- 595 1167 +/- 451 1031 +/- 340 -0.000846 -0.379267 -0.815739 0.3774895 0.8810302 PPA1 pyrophosphatase (inorganic) 1 225 +/- 22 258 +/- 18 214 +/- 15 177 +/- 24 -0.020726 0.2716038 -0.229216 0.2539245 0.5310344 PPAP2B phosphatidic acid phosphatase type 2B 2720 +/- 167 3718 +/- 213 2634 +/- 127 2728 +/- 239 -0.040275 0.0045712 0.74131 0.0631569 0.390909 PPAP2C phosphatidic acid phosphatase type 2C 847 +/- 150 1265 +/- 223 430 +/- 69 630 +/- 61 -0.006536 0.154889 0.057659 0.091321 0.1818752 PPIB peptidylprolyl isomerase B (cyclophilin B) 2898 +/- 79 2835 +/- 89 2361 +/- 89 2393 +/- 73 -0.000109 -0.613527 0.788278 0.7390331 0.6261293 PPIL3 peptidylprolyl isomerase (cyclophilin)-like 3 1204 +/- 34 1212 +/- 75 1026 +/- 46 1101 +/- 64 -0.015401 0.9220126 0.367322 0.5921788 0.7064592 PPM1A protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform 2547 +/- 67 2757 +/- 109 2340 +/- 81 2452 +/- 119 -0.009462 0.1379245 0.459524 0.4371068 0.6589125 PPM1B protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform 2968 +/- 253 3629 +/- 258 2741 +/- 80 2996 +/- 47 -0.046699 0.0972619 0.02543 0.0367724 0.0098338 PPM1K protein phosphatase 1K (PP2C domain containing) 4521 +/- 377 5333 +/- 379 3697 +/- 191 3990 +/- 155 -0.001511 0.1593614 0.26444 0.0447384 0.3057373 PPM1M protein phosphatase 1M (PP2C domain containing) 291 +/- 33 281 +/- 13 182 +/- 6 226 +/- 25 -0.006166 -0.795166 0.161232 0.6497394 0.2206157 PPME1 protein phosphatase methylesterase 1 2999 +/- 69 2959 +/- 132 2515 +/- 61 2564 +/- 44 -0.00063 -0.79469 0.539665 0.1788383 0.4715651 PPP1R14Aprotein phosphatase 1, regulatory (inhibitor) subunit 14A 6747 +/- 1060 8514 +/- 1392 3565 +/- 165 5136 +/- 704 -0.006314 0.3377909 0.088662 0.138628 0.1075956 PPP2R1B protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform 287 +/- 19 260 +/- 12 100 +/- 26 121 +/- 16 -1.07E-05 -0.24549 0.515692 0.3272793 0.2972862 PPP2R2C protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), gamma isoform 1920 +/- 87 1787 +/- 75 1505 +/- 116 1415 +/- 120 -0.000321 -0.275206 -0.604954 0.1711659 0.7534965 PPP2R2D protein phosphatase 2, regulatory subunit B, delta isoform 1244 +/- 31 1237 +/- 39 1121 +/- 49 1173 +/- 32 -0.004433 -0.892091 0.403892 0.9857067 0.3778994 PPP2R3A protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha 964 +/- 135 1257 +/- 136 776 +/- 33 834 +/- 46 -0.009557 0.1567783 0.332014 0.0381379 0.5770586 PPP2R5C protein phosphatase 2, regulatory subunit B (B56), gamma isoform 1380 +/- 94 967 +/- 75 1220 +/- 33 860 +/- 51 -0.02482 -0.006726 -0.000553 0.1080542 0.0232061 PPP3CB protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta) 15152 +/- 1195 12631 +/- 714 12302 +/- 318 11683 +/- 323 -0.038687 -0.106985 -0.205682 0.0930427 0.2706447 PPP4R1 protein phosphatase 4, regulatory subunit 1 635 +/- 45 605 +/- 39 538 +/- 21 525 +/- 28 -0.006451 -0.618645 -0.718113 0.7170786 0.655908 PPP6C protein phosphatase 6, catalytic subunit 1759 +/- 55 1718 +/- 73 1502 +/- 42 1527 +/- 77 -0.001254 -0.662141 0.789272 0.9256708 0.2989036 PQLC3 PQ loop repeat containing 3 422 +/- 15 400 +/- 47 254 +/- 11 298 +/- 12 -0.000134 -0.674626 0.023289 0.696103 0.3334257 PRDX4 peroxiredoxin 4 801 +/- 39 830 +/- 38 686 +/- 17 724 +/- 27 -0.000375 0.5977847 0.260903 0.2923476 0.1129491

S2-genes differentially expressed across region.xls Page 62 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI PRDX5 peroxiredoxin 5 3941 +/- 95 3412 +/- 66 3326 +/- 71 3314 +/- 37 -0.008912 -0.0014 -0.885484 0.0020949 0.9175833 PRG1 proteoglycan 1, secretory granule 1662 +/- 87 2134 +/- 206 1240 +/- 135 1457 +/- 182 -0.002262 0.0739621 0.368565 0.0304929 0.4925677 PRIMA1 proline rich membrane anchor 1 564 +/- 83 750 +/- 125 309 +/- 30 390 +/- 40 -0.004927 0.2483317 0.147625 0.1831599 0.4877595 PRKACB protein kinase, cAMP-dependent, catalytic, beta 495 +/- 144 444 +/- 88 237 +/- 41 214 +/- 32 -0.006964 -0.76968 -0.658152 0.8831969 0.8845366 PRKCB1 Protein kinase C, beta 1 2174 +/- 618 1285 +/- 104 803 +/- 83 774 +/- 98 -0.016372 -0.211927 -0.824718 0.1741265 0.972401 PRKCH protein kinase C, eta 181 +/- 18 255 +/- 18 129 +/- 12 131 +/- 19 -0.000728 0.0168632 0.943452 0.1302874 0.8546068 PRKCQ protein kinase C, theta 608 +/- 116 724 +/- 105 467 +/- 37 538 +/- 25 -0.031235 0.4760915 0.149647 0.1796332 0.0166771 PRKCSH protein kinase C substrate 80K-H 919 +/- 125 1252 +/- 183 631 +/- 105 794 +/- 20 -0.001559 0.1684318 0.18489 0.1086474 0.1416255 PRKCZ protein kinase C, zeta 5442 +/- 328 5041 +/- 226 4396 +/- 91 4519 +/- 86 -0.007149 -0.340031 0.35279 0.1430127 0.2503548 PRMT3 protein arginine methyltransferase 3 829 +/- 57 689 +/- 73 680 +/- 25 559 +/- 59 -0.002615 -0.163433 -0.11103 0.5221687 0.2510679 PRMT5 protein arginine methyltransferase 5 1041 +/- 31 1003 +/- 41 873 +/- 16 910 +/- 28 -0.000445 -0.485184 0.297004 0.1595882 0.8973365 PRMT8 protein arginine methyltransferase 8 282 +/- 50 232 +/- 24 142 +/- 18 151 +/- 19 -0.002489 -0.396408 0.7377 0.1623122 0.9718183 PROM1 prominin 1 550 +/- 67 570 +/- 64 325 +/- 23 328 +/- 33 -0.000107 0.8312817 0.934858 0.2893773 0.3443635 PROS1 protein S (alpha) 774 +/- 108 426 +/- 28 473 +/- 63 375 +/- 23 -0.044059 -0.022169 -0.190453 0.0335408 0.2462449 PRPF4 PRP4 pre-mRNA processing factor 4 homolog (yeast) 512 +/- 6 550 +/- 18 463 +/- 9 479 +/- 12 -0.001825 0.0967279 0.301007 0.4445516 0.733277 PRPF8 PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) 3050 +/- 162 3083 +/- 68 2898 +/- 94 2801 +/- 62 -0.020731 0.8538068 -0.410641 0.8894511 0.671097 PRPS2 phosphoribosyl pyrophosphate synthetase 2 1966 +/- 106 1443 +/- 29 1604 +/- 58 1369 +/- 46 -0.02071 -0.003525 -0.011978 0.0015139 0.084104 PRR13 proline rich 13 3251 +/- 117 3856 +/- 164 2723 +/- 24 3074 +/- 150 -0.000652 0.0147229 0.079234 0.0860497 0.1849899 PRRG1 proline rich Gla (G-carboxyglutamic acid) 1 909 +/- 109 1222 +/- 166 601 +/- 15 713 +/- 29 -0.004497 0.149903 0.01455 0.0265939 0.0612018 PRSS15 protease, serine, 15 878 +/- 21 844 +/- 21 741 +/- 29 658 +/- 38 -0.000308 -0.288104 -0.118514 0.1803022 0.404888 PSCD1 pleckstrin homology, Sec7 and coiled-coil domains 1(cytohesin 1) 542 +/- 28 648 +/- 46 410 +/- 24 447 +/- 23 -0.000233 0.0876713 0.282194 0.0474065 0.1952757 PSCD4 pleckstrin homology, Sec7 and coiled-coil domains 4 213 +/- 21 291 +/- 13 199 +/- 18 202 +/- 16 -0.035829 0.012288 0.924077 0.0949477 0.4844499 PSEN2 presenilin 2 (Alzheimer disease 4) 1262 +/- 63 1124 +/- 22 865 +/- 40 903 +/- 38 -0.000224 -0.083931 0.506342 0.1731793 0.5550119 PSENEN presenilin enhancer 2 homolog (C. elegans) 1358 +/- 137 1479 +/- 87 974 +/- 44 1110 +/- 105 -0.000912 0.4784512 0.281797 0.2749988 0.0857524 PSMA2 proteasome (prosome, macropain) subunit, alpha type, 2 1231 +/- 39 1139 +/- 46 1017 +/- 44 912 +/- 20 -5.25E-05 -0.15732 -0.068328 0.524044 0.3395444 PSMA7 proteasome (prosome, macropain) subunit, alpha type, 7 11663 +/- 491 10451 +/- 417 10379 +/- 187 9857 +/- 468 -0.033198 -0.09008 -0.344795 0.2060846 0.6007155 PSMB10 proteasome (prosome, macropain) subunit, beta type, 10 568 +/- 63 536 +/- 23 443 +/- 82 399 +/- 28 -0.000222 -0.652532 -0.632334 0.3580461 0.3530405 PSMB4 proteasome (prosome, macropain) subunit, beta type, 4 3705 +/- 115 3631 +/- 124 3280 +/- 73 3573 +/- 83 -0.020303 -0.669068 0.027907 0.9954379 0.0416578 PSMB9 proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2 262 +/- 10 349 +/- 12 233 +/- 6 295 +/- 8 -0.001327 0.0002752 0.000367 0.0088962 0.033051 PSMC3 proteasome (prosome, macropain) 26S subunit, ATPase, 3 1589 +/- 109 1605 +/- 70 1331 +/- 76 1442 +/- 85 -0.00218 0.9051648 0.357346 0.5546138 0.4551318 PSMD4 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 2476 +/- 113 2897 +/- 73 2016 +/- 75 2132 +/- 95 -1.49E-05 0.0127068 0.364098 0.0997047 0.5086628 PSMD7 proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog) 2889 +/- 47 2710 +/- 85 2591 +/- 69 2534 +/- 58 -0.006381 -0.101669 -0.545845 0.1067665 0.9168746 PSME1 proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) 1517 +/- 84 1712 +/- 77 1341 +/- 45 1539 +/- 108 -0.005046 0.1185632 0.147611 0.4206797 0.8360293 PSME4 proteasome (prosome, macropain) activator subunit 4 639 +/- 35 600 +/- 46 499 +/- 11 506 +/- 27 -0.00095 -0.520907 0.818529 0.7408671 0.7653912 PSPC1 paraspeckle component 1 4137 +/- 535 3110 +/- 267 3353 +/- 193 2357 +/- 289 -0.016884 -0.127704 -0.023391 0.5110806 0.1583018 PSTPIP1 proline-serine-threonine phosphatase interacting protein 1 194 +/- 23 195 +/- 15 156 +/- 13 126 +/- 14 -0.009109 0.9586673 -0.140863 0.8420173 0.5030265 PTBP1 polypyrimidine tract binding protein 1 992 +/- 109 1105 +/- 62 800 +/- 34 778 +/- 23 -0.002971 0.3957911 -0.60763 0.1908457 0.6374071 PTD008 PTD008 protein 3390 +/- 189 3387 +/- 145 2765 +/- 160 2918 +/- 72 -2.04E-05 -0.989725 0.413223 0.7063254 0.4441046 PTDSR phosphatidylserine receptor 654 +/- 24 621 +/- 28 512 +/- 21 470 +/- 15 -1.87E-05 -0.393163 -0.140472 0.8405425 0.3571908 PTGDS prostaglandin D2 synthase 21kDa (brain) 22511 +/- 2273 24357 +/- 1112 18690 +/- 1056 21104 +/- 809 -0.006496 0.4885458 0.103668 0.6344669 0.5541901 PTGFRN prostaglandin F2 receptor negative regulator 649 +/- 71 794 +/- 81 506 +/- 45 509 +/- 18 -0.003843 0.2064427 0.962011 0.1855553 0.9433773 PTK9 PTK9 protein tyrosine kinase 9 2570 +/- 54 2776 +/- 132 2429 +/- 75 2488 +/- 79 -0.006942 0.1926692 0.597621 0.0710839 0.4014532 PTPDC1 protein tyrosine phosphatase domain containing 1 715 +/- 75 923 +/- 154 399 +/- 37 491 +/- 31 -0.003478 0.2621752 0.088951 0.0680314 0.1366069 PTPLAD2 protein tyrosine phosphatase-like A domain containing 2 114 +/- 22 191 +/- 25 74 +/- 9 99 +/- 11 -0.003222 0.0418422 0.112482 0.0235631 0.4311144 PTPN18 protein tyrosine phosphatase, non-receptor type 18 (brain-derived) 1028 +/- 71 941 +/- 68 849 +/- 67 853 +/- 46 -0.009359 -0.396585 0.9586 0.2677889 0.8432134

S2-genes differentially expressed across region.xls Page 63 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI PTPRB protein tyrosine phosphatase, receptor type, B 193 +/- 17 229 +/- 15 142 +/- 9 176 +/- 12 -0.000313 0.1467456 0.053789 0.1643753 0.1592827 PTPRD Protein tyrosine phosphatase, receptor type, D 3412 +/- 455 4494 +/- 451 2387 +/- 204 2839 +/- 148 -0.000604 0.1221485 0.107674 0.0948477 0.1160613 PTPRR protein tyrosine phosphatase, receptor type, R 696 +/- 106 733 +/- 94 607 +/- 43 552 +/- 47 -0.039739 0.799284 -0.416032 0.7429474 0.1852475 PVALB parvalbumin 391 +/- 24 469 +/- 50 287 +/- 22 418 +/- 28 -0.020259 0.1995472 0.005842 0.5771577 0.0334362 PXDN peroxidasin homolog (Drosophila) 225 +/- 13 204 +/- 15 193 +/- 18 161 +/- 10 -0.011688 -0.313093 -0.155814 0.1028608 0.0343556 PXMP2 peroxisomal membrane protein 2, 22kDa 919 +/- 52 996 +/- 33 853 +/- 37 825 +/- 30 -0.020738 0.2433881 -0.565953 0.276982 0.2095274 PYGB phosphorylase, glycogen; brain 3155 +/- 246 3198 +/- 255 2648 +/- 110 2428 +/- 67 -0.004464 0.9060542 -0.125182 0.8293315 0.1089386 PYGL phosphorylase, glycogen; liver (Hers disease, glycogen storage disease type VI) 160 +/- 29 223 +/- 46 112 +/- 15 126 +/- 25 -0.005056 0.2807569 0.648166 0.0228758 0.0863138 PYGM phosphorylase, glycogen; muscle (McArdle syndrome, glycogen storage disease type V) 1609 +/- 210 1777 +/- 111 1318 +/- 107 1540 +/- 79 -0.011448 0.5008947 0.129767 0.6130123 0.3472768 PYGO2 pygopus homolog 2 (Drosophila) 1108 +/- 143 1141 +/- 41 936 +/- 75 841 +/- 25 -0.015185 0.8329628 -0.274444 0.7454591 0.4034806 QARS glutaminyl-tRNA synthetase 1311 +/- 57 1292 +/- 43 950 +/- 17 929 +/- 22 -1.44E-06 -0.801316 -0.458587 0.7941832 0.5468403 R3HDM2 R3H domain containing 2 3854 +/- 202 3846 +/- 224 3526 +/- 89 3218 +/- 156 -0.020388 -0.980525 -0.134115 0.8413577 0.7046981 RAB11FIP4RAB11 family interacting protein 4 (class II) 4018 +/- 291 4087 +/- 199 3007 +/- 99 3075 +/- 162 -6.13E-05 0.8497989 0.730535 0.9145243 0.2969427 RAB1A RAB1A, member RAS oncogene family 992 +/- 40 1101 +/- 51 862 +/- 26 897 +/- 34 -0.003979 0.1281371 0.44462 0.0674852 0.7252461 RAB27B RAB27B, member RAS oncogene family 2145 +/- 133 1839 +/- 265 1389 +/- 162 1287 +/- 126 -0.000315 -0.334554 -0.632606 0.0790225 0.6702685 RAB32 RAB32, member RAS oncogene family 198 +/- 18 242 +/- 23 137 +/- 8 201 +/- 18 -0.000716 0.1654716 0.020572 0.1703733 0.1214088 RAB35 RAB35, member RAS oncogene family 778 +/- 29 811 +/- 19 718 +/- 14 670 +/- 5 -0.001143 0.3674905 -0.015566 0.5906463 0.0883262 RAB3GAP2RAB3 GTPase activating protein subunit 2 (non-catalytic) 2868 +/- 148 3105 +/- 104 2399 +/- 77 2721 +/- 85 -0.000532 0.2241835 0.020968 0.4857475 0.0543959 RAB6IP1 RAB6 interacting protein 1 1889 +/- 88 1974 +/- 155 1281 +/- 66 1285 +/- 52 -9.7E-05 0.6473273 0.961317 0.3121541 0.6522225 RAB7 RAB7, member RAS oncogene family 9341 +/- 493 9851 +/- 264 7242 +/- 277 8105 +/- 249 -1.27E-05 0.3893238 0.045658 0.7249005 0.2878582 RAB8A RAB8A, member RAS oncogene family 660 +/- 25 807 +/- 25 552 +/- 11 656 +/- 19 -6.22E-05 0.0021892 0.002421 0.0048684 0.0003511 RABAC1 Rab acceptor 1 (prenylated) 3817 +/- 159 3921 +/- 206 2935 +/- 80 3016 +/- 129 -0.000103 0.6989125 0.608635 0.853502 0.7374481 RABEP1 rabaptin, RAB GTPase binding effector protein 1 2142 +/- 134 2463 +/- 95 1858 +/- 114 2298 +/- 159 -0.046629 0.0823083 0.056551 0.1709026 0.2422783 RABEP2 rabaptin, RAB GTPase binding effector protein 2 275 +/- 8 281 +/- 16 254 +/- 9 247 +/- 10 -0.011503 0.7313138 -0.63573 0.1656135 0.760974 RABIF RAB interacting factor 534 +/- 29 645 +/- 54 468 +/- 14 555 +/- 44 -0.000249 0.1105411 0.119867 0.0505082 0.0027574 RABL4 RAB, member of RAS oncogene family-like 4 740 +/- 95 1128 +/- 110 549 +/- 56 835 +/- 106 -0.000823 0.0238958 0.054058 0.0768268 0.3212915 RAD21 RAD21 homolog (S. pombe) 5571 +/- 207 5762 +/- 93 5413 +/- 165 4952 +/- 247 -0.038491 0.4288964 -0.163859 0.2137327 0.2571036 RAG1AP1 recombination activating gene 1 activating protein 1 962 +/- 79 843 +/- 84 777 +/- 37 656 +/- 48 -0.004419 -0.325465 -0.082613 0.2431811 0.0455059 RALA v-ral simian leukemia viral oncogene homolog A (ras related) 2306 +/- 161 2038 +/- 111 1849 +/- 56 1803 +/- 66 -0.006592 -0.204021 -0.611925 0.4712526 0.7060513 RALBP1 ralA binding protein 1 887 +/- 38 1005 +/- 43 826 +/- 18 837 +/- 56 -0.047193 0.0657419 0.850109 0.2570634 0.2129839 RALGDS ral guanine nucleotide dissociation stimulator 1650 +/- 149 1792 +/- 196 970 +/- 61 1034 +/- 81 -0.000114 0.5789536 0.546252 0.1317755 0.2657105 RALY RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse 932 +/- 71 1060 +/- 64 771 +/- 41 809 +/- 23 -0.00125 0.2122587 0.443477 0.2176908 0.5794756 RAN RAN, member RAS oncogene family 5585 +/- 337 5166 +/- 255 4667 +/- 225 5138 +/- 283 -0.020597 -0.346163 0.228518 0.0649918 0.8079327 RANGNRF RAN guanine nucleotide release factor 425 +/- 34 318 +/- 15 318 +/- 17 272 +/- 27 -0.003175 -0.024131 -0.192118 0.0152892 0.0205336 RAP1A RAP1A, member of RAS oncogene family 2755 +/- 114 3035 +/- 157 2475 +/- 162 2521 +/- 63 -0.021004 0.1829802 0.797143 0.050363 0.9605492 RAP2A RAP2A, member of RAS oncogene family 4457 +/- 62 4234 +/- 325 3871 +/- 217 3497 +/- 172 -0.001575 -0.527556 -0.210754 0.9815063 0.4888205 RAP80 receptor associated protein 80 1079 +/- 80 1269 +/- 127 891 +/- 21 956 +/- 15 -0.005113 0.2402403 0.033389 0.3779647 0.1303282 RASA4 RAS p21 protein activator 4 383 +/- 36 354 +/- 23 326 +/- 24 258 +/- 21 -0.010923 -0.514392 -0.05755 0.2802148 0.0195089 RASD1 RAS, dexamethasone-induced 1 3412 +/- 336 3284 +/- 187 2240 +/- 175 2565 +/- 111 -0.002513 -0.748457 0.155101 0.3967154 0.1805933 RASL11B RAS-like, family 11, member B 525 +/- 73 390 +/- 82 142 +/- 22 130 +/- 19 -0.000195 -0.249543 -0.689563 0.3792793 0.8186812 RASSF2 Ras association (RalGDS/AF-6) domain family 2 2060 +/- 315 2292 +/- 384 1182 +/- 82 1158 +/- 79 -0.002153 0.6506082 -0.841192 0.1590444 0.7698049 RASSF3 Ras association (RalGDS/AF-6) domain family 3 1493 +/- 99 1586 +/- 78 1343 +/- 33 1259 +/- 24 -0.004242 0.4811135 -0.06658 0.2075246 0.332505 RASSF4 Ras association (RalGDS/AF-6) domain family 4 725 +/- 66 756 +/- 50 647 +/- 40 670 +/- 16 -0.019642 0.7176493 0.615234 0.3135811 0.1488019 RASSF8 Ras association (RalGDS/AF-6) domain family 8 637 +/- 74 677 +/- 35 597 +/- 48 552 +/- 33 -0.036735 0.639533 -0.457421 0.8227437 0.1629416 RAVER2 ribonucleoprotein, PTB-binding 2 873 +/- 37 787 +/- 47 763 +/- 38 650 +/- 44 -0.009749 -0.185052 -0.083126 0.1142858 0.0794836

S2-genes differentially expressed across region.xls Page 64 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI RB1CC1 RB1-inducible coiled-coil 1 1324 +/- 75 1490 +/- 80 1157 +/- 82 1036 +/- 31 -0.001613 0.1595626 -0.213576 0.0719652 0.6426079 RBBP8 retinoblastoma binding protein 8 127 +/- 15 122 +/- 10 91 +/- 12 96 +/- 17 -0.022267 -0.790124 0.808943 0.6856397 0.15006 RBJ Ras-associated protein Rap1 1529 +/- 36 1613 +/- 61 1371 +/- 54 1460 +/- 62 -0.002764 0.2657031 0.306639 0.7448603 0.2482896 RBKS ribokinase 159 +/- 20 146 +/- 10 130 +/- 9 110 +/- 6 -0.036763 -0.570289 -0.093368 0.719304 0.2386031 RBM14 RNA binding motif protein 14 1342 +/- 61 1512 +/- 53 1256 +/- 76 1219 +/- 78 -0.046067 0.0637591 -0.744553 0.1004658 0.5667873 RBM19 RNA binding motif protein 19 727 +/- 55 514 +/- 46 537 +/- 38 381 +/- 60 -0.002644 -0.014212 -0.062673 0.07568 0.1984864 RBM22 RNA binding motif protein 22 1765 +/- 139 2069 +/- 109 1394 +/- 57 1420 +/- 24 -0.000323 0.1182209 0.694776 0.0939271 0.4587915 RBMS1 RNA binding motif, single stranded interacting protein 1 195 +/- 15 138 +/- 12 149 +/- 8 122 +/- 10 -0.023495 -0.016158 -0.059221 0.0416093 0.2834169 RBPSUH recombining binding protein suppressor of hairless (Drosophila) 2981 +/- 267 3106 +/- 262 2221 +/- 120 2238 +/- 74 -0.001207 0.7461259 0.907723 0.4020175 0.5302816 RCC2 regulator of chromosome condensation 2 1128 +/- 61 1061 +/- 38 1027 +/- 60 1015 +/- 31 -0.045894 -0.373532 -0.867703 0.4987412 0.7242669 RCCD1 RCC1 domain containing 1 175 +/- 24 201 +/- 26 144 +/- 11 168 +/- 12 -0.033671 0.4739111 0.179508 0.2022238 0.2077872 RCN2 reticulocalbin 2, EF-hand calcium binding domain 5726 +/- 226 5999 +/- 126 5453 +/- 322 5211 +/- 80 -0.015756 0.3238108 -0.496805 0.5153718 0.364355 RCOR2 REST corepressor 2 369 +/- 19 382 +/- 29 333 +/- 13 302 +/- 18 -0.01718 0.7200743 -0.210434 0.6945482 0.4149349 RDBP RD RNA binding protein 1187 +/- 21 1219 +/- 110 913 +/- 25 937 +/- 43 -0.003231 0.7847859 0.649046 0.6433342 0.945711 RDH12 retinol dehydrogenase 12 (all-trans/9-cis/11-cis) 452 +/- 34 392 +/- 47 340 +/- 21 351 +/- 59 -0.017986 -0.336189 0.85896 0.0945357 0.9739087 RDX radixin 340 +/- 45 357 +/- 31 213 +/- 7 219 +/- 10 -0.000887 0.7630346 0.612992 0.8472482 0.7219133 REEP1 receptor accessory protein 1 3525 +/- 94 2995 +/- 201 2782 +/- 183 2618 +/- 100 -0.00511 -0.048147 -0.455031 0.0133104 0.8612964 RER1 RER1 retention in endoplasmic reticulum 1 homolog (S. cerevisiae) 2397 +/- 128 2043 +/- 116 1810 +/- 67 1911 +/- 139 -0.000969 -0.067708 0.535953 0.1050865 0.8214577 RFC1 replication factor C (activator 1) 1, 145kDa 456 +/- 21 417 +/- 17 412 +/- 18 386 +/- 26 -0.035333 -0.183259 -0.431201 0.0218956 0.1886658 RFP ret finger protein 1377 +/- 107 1242 +/- 62 1191 +/- 44 1033 +/- 76 -0.033781 -0.306975 -0.117652 0.3543826 0.1006262 RFXANK regulatory factor X-associated ankyrin-containing protein 382 +/- 19 443 +/- 19 312 +/- 22 350 +/- 23 -0.000451 0.0455876 0.256114 0.0324922 0.0503628 RGC32 response gene to complement 32 6859 +/- 127 8305 +/- 653 5556 +/- 183 6802 +/- 669 -0.000298 0.0779701 0.13729 0.3430082 0.646021 RGL1 ral guanine nucleotide dissociation stimulator-like 1 2405 +/- 65 2230 +/- 58 1949 +/- 71 1953 +/- 77 -2.77E-05 -0.07391 0.969413 0.1153387 0.4356903 RGS4 regulator of G-protein signalling 4 1494 +/- 470 1745 +/- 400 548 +/- 133 512 +/- 149 -0.00077 0.6930025 -0.858951 0.9349628 0.8882524 RGS5 regulator of G-protein signalling 5 2558 +/- 229 3114 +/- 232 2237 +/- 166 2519 +/- 210 -0.007642 0.118181 0.322261 0.1959147 0.3067307 RHBDF1 rhomboid 5 homolog 1 (Drosophila) 290 +/- 21 331 +/- 17 256 +/- 15 270 +/- 13 -0.007655 0.1643482 0.492793 0.1366439 0.5157773 RHBDL2 rhomboid, veinlet-like 2 (Drosophila) 159 +/- 38 241 +/- 53 91 +/- 15 121 +/- 8 -0.017273 0.2358206 0.107435 0.0888637 0.2288532 RHEBL1 Ras homolog enriched in brain like 1 228 +/- 26 221 +/- 29 176 +/- 10 154 +/- 10 -0.004442 -0.864525 -0.176486 0.7092064 0.1938841 RHOBTB1 Rho-related BTB domain containing 1 303 +/- 17 310 +/- 25 217 +/- 23 215 +/- 14 -0.001526 0.8045995 -0.948916 0.8143246 0.9841809 RHOQ ras homolog gene family, member Q 1652 +/- 187 2322 +/- 252 1510 +/- 132 2021 +/- 227 -0.025698 0.0605443 0.094703 0.0802522 0.2605742 RHOU ras homolog gene family, member U 2390 +/- 265 3326 +/- 556 1311 +/- 72 1705 +/- 52 -0.001259 0.1711621 0.00185 0.0287121 0.0028013 RHPN2 rhophilin, Rho GTPase binding protein 2 1152 +/- 182 1102 +/- 155 788 +/- 76 715 +/- 84 -0.003049 -0.839588 -0.538191 0.8055805 0.5809222 RICTOR rapamycin-insensitive companion of mTOR 2415 +/- 271 3115 +/- 219 2053 +/- 54 2477 +/- 109 -0.018334 0.073289 0.013266 0.0617395 0.0030748 RIF1 RAP1 interacting factor homolog (yeast) 637 +/- 107 956 +/- 124 515 +/- 28 671 +/- 28 -0.040163 0.080335 0.003329 0.0156319 0.0176562 RIPK2 receptor-interacting serine-threonine kinase 2 475 +/- 23 551 +/- 22 420 +/- 19 435 +/- 19 -0.000169 0.040023 0.590228 0.2592377 0.9694159 RNASE1 ribonuclease, RNase A family, 1 (pancreatic) 2782 +/- 213 3018 +/- 344 1769 +/- 96 1909 +/- 98 -8.04E-05 0.5745971 0.334639 0.3801282 0.1111012 RNASE4 ribonuclease, RNase A family, 4 204 +/- 17 250 +/- 22 133 +/- 12 240 +/- 28 -0.003533 0.1324862 0.015739 0.2850226 0.0024759 RNASE6 ribonuclease, RNase A family, k6 397 +/- 35 654 +/- 73 255 +/- 16 436 +/- 47 -0.003229 0.0149096 0.014506 0.0062424 0.0746811 RNASEH2Aribonuclease H2, large subunit 227 +/- 15 280 +/- 16 209 +/- 14 224 +/- 16 -0.051719 0.0374968 0.500722 0.1277155 0.7681737 RND2 Rho family GTPase 2 570 +/- 97 667 +/- 106 381 +/- 30 457 +/- 41 -0.008905 0.5131604 0.171732 0.3752886 0.3217803 RNF13 ring finger protein 13 3691 +/- 205 4662 +/- 821 2704 +/- 169 3107 +/- 143 -0.004602 0.2979435 0.101574 0.1364455 0.2439547 RNF130 ring finger protein 130 2915 +/- 322 3688 +/- 372 2157 +/- 119 2608 +/- 105 -0.00724 0.1481042 0.019517 0.0619848 0.0806561 RNF150 ring finger protein 150 1265 +/- 67 1161 +/- 47 727 +/- 55 825 +/- 45 -1.7E-05 -0.23155 0.199869 0.1043199 0.1848287 RNF169 ring finger protein 169 832 +/- 65 876 +/- 28 722 +/- 30 699 +/- 25 -0.018221 0.5556799 -0.565262 0.379811 0.9933702 RNF187 ring finger protein 187 4466 +/- 174 4113 +/- 168 4156 +/- 120 3630 +/- 66 -0.038965 -0.175609 -0.005496 0.0383765 0.0309644

S2-genes differentially expressed across region.xls Page 65 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI RNF31 ring finger protein 31 560 +/- 30 585 +/- 27 518 +/- 44 504 +/- 30 -0.014446 0.5524084 -0.791178 0.4145564 0.6720362 RNF4 ring finger protein 4 3001 +/- 120 2944 +/- 147 2498 +/- 80 2543 +/- 127 -0.000177 -0.771791 0.769723 0.1662269 0.5217967 RNF5 ring finger protein 5 2066 +/- 79 2018 +/- 85 1672 +/- 90 1720 +/- 58 -0.001377 -0.689741 0.667614 0.2508549 0.9852024 RNF8 ring finger protein 8 1471 +/- 47 1414 +/- 41 1327 +/- 58 1342 +/- 35 -0.038975 -0.375303 0.821652 0.1086006 0.9210567 RNPC1 RNA-binding region (RNP1, RRM) containing 1 456 +/- 27 478 +/- 42 379 +/- 20 416 +/- 24 -0.021848 0.669057 0.27006 0.2154478 0.0728935 ROBO2 roundabout, axon guidance receptor, homolog 2 (Drosophila) 1844 +/- 48 1630 +/- 69 1649 +/- 73 1537 +/- 82 -0.030465 -0.032174 -0.337925 0.001026 0.6461609 RP11-217Himplantation-associated protein 540 +/- 57 800 +/- 92 433 +/- 35 574 +/- 40 -0.025838 0.0414708 0.027973 0.004277 0.1483887 RP11-529I deleted in a mouse model of primary ciliary dyskinesia 1563 +/- 158 1588 +/- 71 1256 +/- 136 1535 +/- 70 -0.027485 0.8908397 0.108769 0.7478505 0.3240476 RP11-82K1kynurenine aminotransferase III 1081 +/- 48 1236 +/- 69 926 +/- 68 993 +/- 30 -0.003627 0.0977327 0.397431 0.0530337 0.3786187 RPL10A ribosomal protein L10a 8674 +/- 630 9506 +/- 500 7539 +/- 549 8750 +/- 404 -0.001703 0.3264763 0.11057 0.3804491 0.1314289 RPL12 ribosomal protein L12 9430 +/- 547 8804 +/- 725 7866 +/- 527 7907 +/- 590 -1.14E-05 -0.50805 0.959344 0.3907329 0.8422057 RPL13 ribosomal protein L13 3331 +/- 242 4584 +/- 286 2546 +/- 122 3520 +/- 104 -9.29E-05 0.0076985 0.000189 0.0087231 0.0059545 RPL13A ribosomal protein L13a 17473 +/- 716 18114 +/- 433 15464 +/- 747 17201 +/- 373 -0.000835 0.4649311 0.074644 0.6891465 0.198273 RPL18 ribosomal protein L18 10779 +/- 411 11534 +/- 392 8934 +/- 293 9623 +/- 357 -8.83E-07 0.2127203 0.173178 0.5068458 0.4955911 RPL19 ribosomal protein L19 9357 +/- 185 10005 +/- 306 8787 +/- 293 9412 +/- 306 -0.002193 0.1062607 0.174019 0.0809968 0.0496284 RPL24 ribosomal protein L24 6992 +/- 211 7638 +/- 185 6738 +/- 313 6697 +/- 239 -0.021558 0.0444177 -0.919546 0.1335962 0.5753282 RPL26L1 ribosomal protein L26-like 1 2945 +/- 149 2782 +/- 137 2548 +/- 62 2607 +/- 60 -0.024552 -0.438809 0.509001 0.0937983 0.8778367 RPL27 ribosomal protein L27 13413 +/- 461 13705 +/- 419 10512 +/- 356 11855 +/- 499 -2.71E-05 0.6494446 0.061547 0.8221047 0.0243609 RPL35 ribosomal protein L35 10578 +/- 573 10858 +/- 301 8520 +/- 386 8832 +/- 240 -7.58E-07 0.6766814 0.510725 0.7943887 0.567852 RPL9 ribosomal protein L9 12030 +/- 543 14358 +/- 285 10191 +/- 563 9811 +/- 1825 -0.000122 0.0062042 -0.850374 0.0454903 0.908068 RPLP1 ribosomal protein, large, P1 17390 +/- 565 17107 +/- 538 15240 +/- 465 16379 +/- 611 -0.012218 -0.724215 0.17693 0.7472193 0.2154918 RPN1 ribophorin I 3214 +/- 165 3122 +/- 98 2919 +/- 210 3018 +/- 46 -0.025388 -0.64072 0.663249 0.4883486 0.6726586 RPN2 ribophorin II 3882 +/- 259 3547 +/- 134 3085 +/- 71 2861 +/- 98 -0.000779 -0.285888 -0.102355 0.4366176 0.093173 RPP21 ribonuclease P 21kDa subunit 734 +/- 18 738 +/- 38 594 +/- 26 589 +/- 26 -0.000119 0.9279823 -0.900898 0.6494391 0.3300117 RPS12 ribosomal protein S12 4329 +/- 211 3150 +/- 239 3422 +/- 186 2508 +/- 191 -0.000234 -0.004219 -0.007636 0.0932139 0.1503967 RPS13 ribosomal protein S13 16142 +/- 483 16738 +/- 421 14590 +/- 129 15112 +/- 432 -0.00047 0.3746461 0.302058 0.4992474 0.6660365 RPS15 ribosomal protein S15 10399 +/- 364 11238 +/- 397 8679 +/- 352 9306 +/- 297 -1.3E-05 0.149955 0.206866 0.1198067 0.0973583 RPS15A ribosomal protein S15a 14626 +/- 477 16659 +/- 556 11527 +/- 341 13684 +/- 211 -3.97E-06 0.019978 0.000642 0.0187529 0.0088016 RPS16 ribosomal protein S16 6925 +/- 336 6071 +/- 172 5467 +/- 306 4710 +/- 188 -1.69E-06 -0.055727 -0.067967 0.1166208 0.2461751 RPS17 ribosomal protein S17 15133 +/- 550 14659 +/- 548 12410 +/- 409 12221 +/- 313 -2.6E-05 -0.555233 -0.722046 0.6327906 0.9831168 RPS18 ribosomal protein S18 16692 +/- 562 16817 +/- 293 14381 +/- 399 14595 +/- 334 -2.53E-06 0.8488996 0.689688 0.8384108 0.8751669 RPS19 ribosomal protein S19 13041 +/- 438 13747 +/- 408 10634 +/- 404 11010 +/- 220 -1.67E-06 0.2654267 0.438195 0.1167424 0.3878737 RPS19BP1ribosomal protein S19 binding protein 1 1321 +/- 154 1432 +/- 192 1046 +/- 59 1038 +/- 73 -0.010934 0.6605775 -0.932222 0.8738857 0.5374471 RPS20 ribosomal protein S20 14369 +/- 692 16473 +/- 680 11954 +/- 708 13764 +/- 510 -5.35E-05 0.0551395 0.069241 0.079733 0.1577603 RPS26 ribosomal protein S26 13524 +/- 616 13145 +/- 731 11557 +/- 620 11245 +/- 406 -1.66E-06 -0.699809 -0.684406 0.8656369 0.9429498 RPS3 ribosomal protein S3 17172 +/- 407 17830 +/- 319 15180 +/- 725 16590 +/- 466 -0.006142 0.2338888 0.139541 0.4730761 0.2048544 RPS3A ribosomal protein S3A 18537 +/- 735 19266 +/- 623 16671 +/- 703 16606 +/- 768 -6.23E-05 0.4669776 -0.952102 0.4531818 0.7517223 RPS4X ribosomal protein S4, X-linked 9260 +/- 677 10466 +/- 375 7253 +/- 542 8406 +/- 347 -0.002163 0.1586952 0.109686 0.1959557 0.1845735 RPS6KA2 ribosomal protein S6 kinase, 90kDa, polypeptide 2 2685 +/- 589 3697 +/- 536 1477 +/- 86 1670 +/- 72 -0.002546 0.2332351 0.119535 0.247605 0.1223245 RPS6KL1 ribosomal protein S6 kinase-like 1 1701 +/- 59 1554 +/- 51 1265 +/- 36 1177 +/- 44 -9.84E-06 -0.088499 -0.155107 0.0267874 0.1540367 RPS8 ribosomal protein S8 7154 +/- 320 7698 +/- 258 6230 +/- 225 6609 +/- 223 -0.000455 0.2167886 0.261404 0.329606 0.1804054 RQCD1 RCD1 required for cell differentiation1 homolog (S. pombe) 501 +/- 35 445 +/- 17 432 +/- 30 405 +/- 21 -0.018195 -0.189014 -0.486167 0.1893383 0.360913 RRAGC Ras-related GTP binding C 2912 +/- 161 3207 +/- 157 2579 +/- 91 2330 +/- 56 -0.000557 0.2207537 -0.047748 0.4301719 0.0631591 RRP22 RAS-related on chromosome 22 2248 +/- 321 1726 +/- 119 1139 +/- 106 977 +/- 76 -0.000973 -0.175486 -0.246375 0.1208223 0.7171852 RSNL2 restin-like 2 1784 +/- 121 1614 +/- 119 1325 +/- 39 1200 +/- 54 -0.000334 -0.341303 -0.100024 0.9270609 0.1793657

S2-genes differentially expressed across region.xls Page 66 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI RSPO2 R-spondin 2 homolog (Xenopus laevis) 596 +/- 111 506 +/- 102 399 +/- 67 387 +/- 44 -0.023991 -0.562046 -0.87551 0.3345013 0.952499 RSPRY1 ring finger and SPRY domain containing 1 2028 +/- 269 2432 +/- 220 1462 +/- 115 1593 +/- 128 -0.001896 0.2720152 0.467212 0.2797266 0.4121253 RTKN rhotekin 1246 +/- 231 1567 +/- 284 660 +/- 62 696 +/- 58 -0.004647 0.4011988 0.68267 0.1203308 0.3029594 RWDD2 RWD domain containing 2 370 +/- 11 338 +/- 12 325 +/- 8 330 +/- 13 -0.008486 -0.080304 0.726507 0.0406136 0.9149342 RXFP1 relaxin/insulin-like family peptide receptor 1 591 +/- 226 702 +/- 201 175 +/- 61 122 +/- 31 -0.002859 0.7223694 -0.4598 0.7890036 0.5376481 S100A13 S100 calcium binding protein A13 1569 +/- 113 1603 +/- 85 1364 +/- 110 1346 +/- 39 -0.001511 0.8122238 -0.882788 0.6478642 0.6144458 S100A6 S100 calcium binding protein A6 (calcyclin) 923 +/- 64 1156 +/- 72 682 +/- 81 1085 +/- 58 -0.00207 0.0359624 0.003149 0.2202466 0.1084609 SAMSN1 SAM domain, SH3 domain and nuclear localisation signals, 1 178 +/- 18 168 +/- 12 131 +/- 17 151 +/- 9 -0.033264 -0.655748 0.337741 0.8102787 0.6358359 SAP130 Sin3A-associated protein, 130kDa 1623 +/- 42 1504 +/- 24 1469 +/- 39 1472 +/- 40 -0.005698 -0.03916 0.964552 0.0232187 0.486987 SAP18 Sin3A-associated protein, 18kDa 2878 +/- 140 4280 +/- 172 2600 +/- 139 3721 +/- 168 -0.020479 0.0001016 0.000789 9.322E-05 0.0050428 SAR1B SAR1 gene homolog B (S. cerevisiae) 378 +/- 14 408 +/- 29 334 +/- 20 328 +/- 7 -0.005747 0.3659433 -0.78977 0.1761479 0.8803547 SART1 squamous cell carcinoma antigen recognised by T cells 480 +/- 21 534 +/- 37 435 +/- 30 422 +/- 17 -0.019021 0.2315699 -0.715831 0.2269534 0.8099678 SAT2 spermidine/spermine N1-acetyltransferase 2 2339 +/- 140 2895 +/- 304 2086 +/- 161 2480 +/- 236 -0.01743 0.1407012 0.208904 0.3660211 0.547859 SCAND1 SCAN domain containing 1 1972 +/- 139 1711 +/- 96 1540 +/- 65 1425 +/- 30 -0.000341 -0.157718 -0.14949 0.2564767 0.1738159 SCAP1 src family associated phosphoprotein 1 230 +/- 42 137 +/- 25 48 +/- 22 45 +/- 9 -0.000443 -0.092402 -0.906312 0.1152447 0.9464595 SCARB2 scavenger receptor class B, member 2 223 +/- 19 306 +/- 41 195 +/- 9 222 +/- 13 -0.032468 0.111617 0.144392 0.0329419 0.0354211 SCRG1 scrapie responsive protein 1 2376 +/- 217 2095 +/- 125 1932 +/- 160 1732 +/- 278 -0.04466 -0.295179 -0.5549 0.2679309 0.7386949 SCRIB scribbled homolog (Drosophila) 1091 +/- 125 1365 +/- 127 994 +/- 50 944 +/- 48 -0.043245 0.155152 -0.488183 0.1363655 0.5885018 SCRN1 secernin 1 9838 +/- 238 10891 +/- 605 8473 +/- 141 10001 +/- 220 -0.003911 0.152799 0.000635 0.8102889 0.0171262 SDCCAG10serologically defined colon cancer antigen 10 894 +/- 29 887 +/- 42 836 +/- 34 773 +/- 18 -0.044456 -0.889559 -0.139259 0.4090283 0.1312519 SDF4 stromal cell derived factor 4 3206 +/- 87 3305 +/- 145 2800 +/- 67 2975 +/- 51 -0.003943 0.5759365 0.067073 0.4892865 0.2434686 SDHC succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa 1763 +/- 91 1634 +/- 45 1610 +/- 52 1461 +/- 35 -0.000608 -0.242924 -0.043667 0.2746808 0.025762 SDK2 sidekick homolog 2 (chicken) 931 +/- 129 836 +/- 94 667 +/- 46 527 +/- 27 -0.009243 -0.563399 -0.030214 0.5199325 0.0915624 SEC23B Sec23 homolog B (S. cerevisiae) 1038 +/- 61 1069 +/- 58 923 +/- 23 1057 +/- 33 -0.045226 0.7178976 0.0106 0.7702871 0.0156109 SELI selenoprotein I 1692 +/- 39 1602 +/- 60 1526 +/- 45 1504 +/- 69 -0.001468 -0.24377 -0.795643 0.0630807 0.2380834 SELK selenoprotein K 3585 +/- 88 3497 +/- 51 3262 +/- 116 3310 +/- 125 -0.002919 -0.417087 0.786128 0.3954927 0.9427398 SELPLG selectin P ligand 660 +/- 60 886 +/- 79 469 +/- 46 573 +/- 50 -0.001155 0.047568 0.159001 0.0551214 0.3252283 SELS selenoprotein S 2984 +/- 127 2979 +/- 209 2303 +/- 53 2615 +/- 85 -0.002081 -0.98617 0.017136 0.7899292 0.0790283 SEMA6A Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A 212 +/- 31 323 +/- 46 169 +/- 22 220 +/- 19 -0.017338 0.0755396 0.108891 0.0161403 0.2971259 SEMA6D sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D 2458 +/- 259 2944 +/- 376 1878 +/- 109 1873 +/- 110 -0.004932 0.3152062 -0.977008 0.1669905 0.6277085 SENP2 SUMO1/sentrin/SMT3 specific peptidase 2 1325 +/- 60 1350 +/- 58 1227 +/- 36 1342 +/- 55 -0.033713 0.762104 0.121099 0.4703381 0.4481699 SENP5 SUMO1/sentrin specific peptidase 5 2276 +/- 80 2148 +/- 38 2131 +/- 52 2107 +/- 34 -0.049266 -0.189824 -0.704969 0.1497195 0.6790794 SEP15 15 kDa selenoprotein 5824 +/- 161 6027 +/- 301 5348 +/- 180 5501 +/- 151 -0.009826 0.5706167 0.53304 0.1874084 0.7205999 SEPHS2 selenophosphate synthetase 2 1665 +/- 90 1673 +/- 73 1356 +/- 51 1441 +/- 41 -0.004414 0.9464308 0.229268 0.8042592 0.5104553 SEPT10 septin 10 585 +/- 55 750 +/- 93 369 +/- 49 487 +/- 43 -0.004941 0.1630644 0.102702 0.035267 0.2214484 SEPT2 septin 2 9480 +/- 477 10693 +/- 414 8162 +/- 297 8305 +/- 230 -0.001421 0.0843496 0.712153 0.0353315 0.6665899 SEPT4 septin 4 2139 +/- 390 3276 +/- 670 986 +/- 142 1503 +/- 193 -0.00558 0.1800351 0.064105 0.0298758 0.054253 SEPT6 septin 6 119 +/- 9 143 +/- 8 83 +/- 7 98 +/- 6 -6.08E-05 0.0760879 0.174252 0.301611 0.4387799 SEPT8 septin 8 2774 +/- 489 5315 +/- 965 1763 +/- 94 3266 +/- 249 -0.02563 0.0491891 0.002205 0.0143031 0.0038959 SERF2 Small EDRK-rich factor 2 471 +/- 44 530 +/- 28 389 +/- 17 433 +/- 17 -0.025532 0.2905828 0.105805 0.0933809 0.3224705 SERINC5 Serine incorporator 5 1915 +/- 212 2175 +/- 321 1061 +/- 102 977 +/- 33 -0.001017 0.5177342 -0.461578 0.1427016 0.5419836 SERP1 stress-associated endoplasmic reticulum protein 1 1318 +/- 69 1146 +/- 64 1052 +/- 40 1059 +/- 58 -0.002948 -0.099627 0.930534 0.5212441 0.4222426 SERPINF1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor 508 +/- 96 868 +/- 124 280 +/- 49 579 +/- 121 -0.001876 0.0462446 0.067889 0.2048228 0.1776994 SERPINI1 serpin peptidase inhibitor, clade I (neuroserpin), member 1 4281 +/- 494 4810 +/- 769 2709 +/- 429 2006 +/- 287 -0.00065 0.5775638 -0.20904 0.5307427 0.6041588 SESN3 sestrin 3 200 +/- 26 188 +/- 12 153 +/- 7 151 +/- 16 -0.00382 -0.682553 -0.924363 0.5468163 0.634063

S2-genes differentially expressed across region.xls Page 67 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI SESTD1 SEC14 and spectrin domains 1 1606 +/- 83 1779 +/- 95 1506 +/- 58 1574 +/- 53 -0.039507 0.2005865 0.41066 0.2309524 0.464126 SF3A3 Splicing factor 3a, subunit 3, 60kDa 3426 +/- 274 2814 +/- 133 2964 +/- 63 2412 +/- 70 -0.010701 -0.082988 -0.000278 0.0193853 0.0052495 SF3B5 splicing factor 3b, subunit 5, 10kDa 2837 +/- 201 2672 +/- 214 2213 +/- 144 2343 +/- 106 -0.000564 -0.585189 0.484432 0.5448035 0.6522958 SFRP1 secreted frizzled-related protein 1 378 +/- 122 475 +/- 148 162 +/- 54 156 +/- 36 -0.010604 0.623064 -0.927847 0.2028574 0.6036077 SFXN1 sideroflexin 1 947 +/- 84 649 +/- 46 800 +/- 33 630 +/- 29 -0.014836 -0.014754 -0.004025 0.0228448 0.0559987 SGK serum/glucocorticoid regulated kinase 1969 +/- 150 2626 +/- 225 1220 +/- 179 1632 +/- 102 -0.000539 0.0390527 0.08161 0.0471041 0.2118836 SGPP1 sphingosine-1-phosphate phosphatase 1 1758 +/- 95 1914 +/- 54 1672 +/- 50 1726 +/- 58 -0.046787 0.191944 0.49523 0.2417377 0.7470477 SH3BP4 SH3-domain binding protein 4 814 +/- 96 1038 +/- 89 566 +/- 21 669 +/- 39 -0.000986 0.11864 0.056439 0.0759538 0.0418179 SH3GLB1 SH3-domain GRB2-like endophilin B1 567 +/- 50 626 +/- 33 510 +/- 25 528 +/- 24 -0.032648 0.3511974 0.616778 0.3175337 0.6337238 SH3KBP1 SH3-domain kinase binding protein 1 986 +/- 71 824 +/- 87 753 +/- 42 615 +/- 74 -0.00691 -0.181956 -0.153524 0.5901198 0.4450613 SH3MD4 SH3 multiple domains 4 1732 +/- 142 1583 +/- 171 1400 +/- 71 1205 +/- 43 -0.003383 -0.518297 -0.047787 0.9282667 0.2591898 SH3PX3 SH3 and PX domain containing 3 243 +/- 24 325 +/- 26 209 +/- 22 227 +/- 14 -0.015925 0.0443613 0.515619 0.0464582 0.7937885 SH3YL1 SH3 domain containing, Ysc84-like 1 (S. cerevisiae) 293 +/- 15 244 +/- 29 225 +/- 18 220 +/- 33 -0.006978 -0.172553 -0.896661 0.1078019 0.5187248 SHC1 SHC (Src homology 2 domain containing) transforming protein 1 1132 +/- 63 1127 +/- 51 787 +/- 49 779 +/- 16 -1.05E-07 -0.953187 -0.879671 0.4632275 0.6804978 SHC4 SHC (Src homology 2 domain containing) family, member 4 124 +/- 15 167 +/- 22 90 +/- 8 111 +/- 5 -0.010218 0.1410114 0.058293 0.0459135 0.0739981 SHE Src homology 2 domain containing E 156 +/- 17 142 +/- 16 93 +/- 18 115 +/- 11 -0.009505 -0.579007 0.333889 0.6488268 0.3167174 SHKBP1 SH3KBP1 binding protein 1 410 +/- 53 488 +/- 37 264 +/- 16 289 +/- 20 -0.000591 0.2601923 0.373108 0.4243334 0.575259 SIAHBP1 fuse-binding protein-interacting repressor 4107 +/- 169 3884 +/- 157 3813 +/- 111 3601 +/- 140 -0.02899 -0.356573 -0.269114 0.2963646 0.4423939 SIL1 SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae) 661 +/- 37 651 +/- 43 520 +/- 24 516 +/- 26 -0.005778 -0.858568 -0.892754 0.7061563 0.4682069 SITPEC signaling intermediate in Toll pathway, evolutionarily conserved 863 +/- 62 845 +/- 22 736 +/- 29 761 +/- 22 -0.013172 -0.799523 0.505429 0.5759923 0.7518272 SIVA CD27-binding (Siva) protein 668 +/- 39 649 +/- 86 405 +/- 38 508 +/- 38 -0.000339 -0.845799 0.085813 0.3055674 0.7244419 SLC12A2 solute carrier family 12 (sodium/potassium/chloride transporters), member 2 528 +/- 75 635 +/- 104 328 +/- 46 433 +/- 45 -0.005046 0.4271903 0.138724 0.2139427 0.2095361 SLC12A7 solute carrier family 12 (potassium/chloride transporters), member 7 242 +/- 22 238 +/- 20 199 +/- 16 171 +/- 18 -0.01019 -0.90519 -0.269562 0.4645587 0.7554538 SLC15A3 solute carrier family 15, member 3 255 +/- 27 313 +/- 19 234 +/- 7 271 +/- 8 -0.020326 0.1124988 0.005854 0.1031174 0.0062236 SLC16A1 solute carrier family 16 (monocarboxylic acid transporters), member 1 180 +/- 12 227 +/- 25 163 +/- 14 144 +/- 13 -0.030079 0.1247604 -0.341952 0.0204312 0.5040037 SLC1A4 solute carrier family 1 (glutamate/neutral amino acid transporter), member 4 3466 +/- 213 3097 +/- 143 3093 +/- 119 2554 +/- 111 -0.007805 -0.185181 -0.009141 0.0994061 0.0194672 SLC22A15 solute carrier family 22 (organic cation transporter), member 15 462 +/- 24 483 +/- 46 394 +/- 24 391 +/- 22 -0.009614 0.7010988 -0.928356 0.5040354 0.8838533 SLC22A18 solute carrier family 22 (organic cation transporter), member 18 269 +/- 23 400 +/- 39 191 +/- 11 317 +/- 21 -0.002158 0.0198389 0.00162 0.1136965 0.0449552 SLC25A10 solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10 192 +/- 8 212 +/- 12 175 +/- 14 177 +/- 12 -0.041889 0.1817923 0.91544 0.1332509 0.4288061 SLC25A12 solute carrier family 25 (mitochondrial carrier, Aralar), member 12 623 +/- 45 584 +/- 47 551 +/- 47 553 +/- 39 -0.026332 -0.555544 0.975908 0.201819 0.4583938 SLC25A26 solute carrier family 25, member 26 945 +/- 55 958 +/- 43 853 +/- 42 773 +/- 58 -0.025582 0.8486563 -0.298285 0.3796062 0.5763735 SLC25A27 solute carrier family 25, member 27 2459 +/- 165 2633 +/- 172 2360 +/- 76 2160 +/- 127 -0.04084 0.4840725 -0.221118 0.4522667 0.4764481 SLC25A36 Solute carrier family 25, member 36 2741 +/- 70 2971 +/- 126 2298 +/- 105 2441 +/- 54 -8.67E-05 0.1500621 0.26298 0.5027017 0.7056145 SLC27A5 solute carrier family 27 (fatty acid transporter), member 5 151 +/- 19 178 +/- 11 114 +/- 5 141 +/- 13 -0.001421 0.2585592 0.09764 0.3084692 0.007251 SLC29A1 solute carrier family 29 (nucleoside transporters), member 1 289 +/- 31 319 +/- 33 213 +/- 10 240 +/- 16 -0.001194 0.524223 0.189616 0.9310837 0.094163 SLC30A1 Solute carrier family 30 (zinc transporter), member 1 1715 +/- 48 1708 +/- 50 1238 +/- 51 1294 +/- 16 -3.34E-07 -0.927111 0.337576 0.8248866 0.3439634 SLC30A9 solute carrier family 30 (zinc transporter), member 9 2587 +/- 153 2562 +/- 105 2326 +/- 46 2421 +/- 128 -0.018456 -0.896299 0.512071 0.7667366 0.6676872 SLC32A1 solute carrier family 32 (GABA vesicular transporter), member 1 1003 +/- 65 920 +/- 118 863 +/- 64 859 +/- 29 -0.049537 -0.552654 -0.957181 0.1885677 0.9633984 SLC35A1 solute carrier family 35 (CMP-sialic acid transporter), member A1 2716 +/- 151 2471 +/- 132 2296 +/- 72 2051 +/- 114 -0.003963 -0.249954 -0.11229 0.4729585 0.2116098 SLC35F1 solute carrier family 35, member F1 3034 +/- 284 2519 +/- 280 1601 +/- 180 1193 +/- 132 -2.85E-05 -0.224771 -0.101401 0.2302804 0.4519835 SLC36A1 solute carrier family 36 (proton/amino acid symporter), member 1 489 +/- 32 475 +/- 38 407 +/- 37 431 +/- 40 -0.036443 -0.784179 0.672771 0.3847319 0.975655 SLC38A3 solute carrier family 38, member 3 745 +/- 45 672 +/- 64 609 +/- 29 564 +/- 43 -0.012786 -0.372483 -0.419096 0.7895595 0.6099201 SLC38A6 solute carrier family 38, member 6 241 +/- 14 266 +/- 14 203 +/- 15 222 +/- 9 -0.018292 0.2465405 0.308043 0.4574683 0.3006164 SLC39A9 solute carrier family 39 (zinc transporter), member 9 1773 +/- 64 1724 +/- 49 1546 +/- 30 1624 +/- 34 -0.003627 -0.558111 0.120269 0.5466566 0.2848608 SLC44A1 solute carrier family 44, member 1 1861 +/- 227 2331 +/- 464 1230 +/- 177 1377 +/- 94 -0.018005 0.3917113 0.486105 0.0649311 0.2689055

S2-genes differentially expressed across region.xls Page 68 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI SLC44A3 solute carrier family 44, member 3 321 +/- 39 318 +/- 17 284 +/- 18 283 +/- 13 -0.024975 -0.951476 -0.972706 0.8818561 0.8996318 SLC45A3 solute carrier family 45, member 3 194 +/- 24 304 +/- 46 108 +/- 28 201 +/- 22 -0.005552 0.0675492 0.027744 0.0448504 0.0220202 SLC4A1APsolute carrier family 4 (anion exchanger), member 1, adaptor protein 1272 +/- 54 1197 +/- 48 1085 +/- 48 1152 +/- 31 -0.030609 -0.322699 0.280839 0.1058573 0.4778692 SLC4A2 solute carrier family 4, anion exchanger, member 2 (erythrocyte membrane protein band 3 882 +/- 184 733 +/- 68 510 +/- 10 535 +/- 34 -0.01687 -0.473852 0.504634 0.8536538 0.2047179 SLC4A8 solute carrier family 4, sodium bicarbonate cotransporter, member 8 334 +/- 22 396 +/- 44 270 +/- 15 324 +/- 20 -0.015693 0.2468422 0.069122 0.0192412 0.0744722 SLC5A11 solute carrier family 5 (sodium/glucose cotransporter), member 11 675 +/- 128 1122 +/- 169 389 +/- 31 607 +/- 40 -0.006695 0.063233 0.002558 0.0506597 0.0129141 SLC5A6 solute carrier family 5 (sodium-dependent vitamin transporter), member 6 537 +/- 27 477 +/- 33 374 +/- 28 342 +/- 21 -0.00115 -0.189725 -0.39002 0.1713593 0.3949025 SLC7A9 solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 985 +/- 116 1241 +/- 290 513 +/- 53 577 +/- 51 -0.001799 0.4417194 0.405934 0.1343201 0.1256434 SLC8A1 solute carrier family 8 (sodium/calcium exchanger), member 1 687 +/- 85 552 +/- 40 481 +/- 78 461 +/- 44 -0.04709 -0.192116 -0.826151 0.0618767 0.7463658 SLC8A3 Solute carrier family 8 (sodium-calcium exchanger), member 3 119 +/- 26 152 +/- 25 84 +/- 8 75 +/- 13 -0.003391 0.3761061 -0.57588 0.1920385 0.708155 SLCO1C1 solute carrier organic anion transporter family, member 1C1 525 +/- 72 446 +/- 45 404 +/- 18 280 +/- 27 -0.008426 -0.380552 -0.006315 0.5831536 0.0395767 SLCO3A1 solute carrier organic anion transporter family, member 3A1 1006 +/- 122 1086 +/- 66 881 +/- 69 914 +/- 56 -0.035836 0.5790633 0.721966 0.3956251 0.7333596 SLTM SAFB-like, transcription modulator 1522 +/- 138 1552 +/- 40 1300 +/- 34 1309 +/- 40 -0.005845 0.8397678 0.866296 0.6270067 0.5689292 SMAD2 SMAD, mothers against DPP homolog 2 (Drosophila) 215 +/- 25 236 +/- 12 154 +/- 11 179 +/- 13 -0.00502 0.4716141 0.177536 0.3326621 0.5184842 SMAP1 stromal membrane-associated protein 1 1839 +/- 57 1785 +/- 100 1664 +/- 91 1630 +/- 56 -0.009411 -0.653278 -0.758078 0.196703 0.443799 SMAP1L stromal membrane-associated protein 1-like 3627 +/- 234 3974 +/- 202 3163 +/- 165 3575 +/- 309 -0.008983 0.2880253 0.282115 0.3261457 0.1099691 SMARCD3 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, 2372 +/- 277 2462 +/- 119 2130 +/- 163 2183 +/- 151 -0.040129 0.7756842 0.817176 0.7342855 0.9321508 SMTN smoothelin 114 +/- 10 129 +/- 10 91 +/- 7 86 +/- 7 -0.005809 0.3001178 -0.637821 0.037805 0.5812242 SNAPAP SNAP-associated protein 2027 +/- 47 2062 +/- 110 1747 +/- 37 1758 +/- 101 -0.001517 0.7746385 0.917984 0.244101 0.9701145 SNF1LK SNF1-like kinase 448 +/- 53 272 +/- 52 268 +/- 49 172 +/- 19 -0.002689 -0.038141 -0.114625 0.0501826 0.2906952 SNF1LK2 SNF1-like kinase 2 558 +/- 25 484 +/- 38 392 +/- 21 359 +/- 26 -0.000225 -0.140076 -0.347504 0.3704946 0.2727385 SNORA24 Small nucleolar RNA, H/ACA box 24 4609 +/- 197 5659 +/- 543 3508 +/- 255 4534 +/- 188 -0.00027 0.1169872 0.010709 0.2964168 0.0667392 SNRPA small nuclear ribonucleoprotein polypeptide A 911 +/- 13 1003 +/- 42 800 +/- 43 792 +/- 37 -0.00124 0.0801748 -0.900706 0.0167262 0.7913614 SNRPC small nuclear ribonucleoprotein polypeptide C 2169 +/- 169 2013 +/- 122 1712 +/- 77 1620 +/- 72 -0.001356 -0.475964 -0.410965 0.4789804 0.3509827 SNRPD2 small nuclear ribonucleoprotein D2 polypeptide 16.5kDa 5044 +/- 192 5118 +/- 280 4200 +/- 80 4327 +/- 97 -0.000411 0.8318711 0.339606 0.8300159 0.3711196 SNRPE small nuclear ribonucleoprotein polypeptide E 2808 +/- 54 2931 +/- 100 2631 +/- 81 2805 +/- 26 -0.028664 0.3104969 0.087745 0.7075338 0.0803164 SNX10 sorting nexin 10 2827 +/- 121 2812 +/- 104 2703 +/- 159 2491 +/- 109 -0.024538 -0.927785 -0.299942 0.8574633 0.4587122 SNX11 sorting nexin 11 881 +/- 30 952 +/- 31 830 +/- 30 882 +/- 25 -0.007082 0.1267566 0.215322 0.5910406 0.8996539 SNX15 sorting nexin 15 663 +/- 53 776 +/- 37 621 +/- 28 587 +/- 40 -0.026805 0.1151833 -0.501619 0.0903526 0.5747247 SNX17 sorting nexin 17 2369 +/- 40 2361 +/- 64 1963 +/- 54 2132 +/- 51 -1.88E-05 -0.920823 0.050425 0.208648 0.3840665 SNX27 sorting nexin family member 27 2232 +/- 103 2045 +/- 65 2055 +/- 71 1925 +/- 38 -0.004032 -0.162788 -0.146211 0.108063 0.094232 SNX30 sorting nexin family member 30 880 +/- 79 1048 +/- 104 795 +/- 47 857 +/- 33 -0.038655 0.2296862 0.315384 0.1273127 0.2177501 SNX6 sorting nexin 6 2150 +/- 178 2464 +/- 218 1646 +/- 80 1831 +/- 137 -0.003101 0.2911647 0.284639 0.1116551 0.4438647 SOD1 superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) 15028 +/- 667 14469 +/- 400 13419 +/- 438 14140 +/- 462 -0.045138 -0.492015 0.286938 0.3010988 0.7549947 SON SON DNA binding protein 2932 +/- 117 3228 +/- 54 2710 +/- 123 2947 +/- 45 -0.042723 0.0552217 0.119386 0.0395948 0.3818334 SORCS3 sortilin-related VPS10 domain containing receptor 3 635 +/- 81 577 +/- 90 436 +/- 27 409 +/- 29 -0.000868 -0.638102 -0.509089 0.4266451 0.3580386 SOSTDC1 sclerostin domain containing 1 136 +/- 46 171 +/- 40 63 +/- 9 50 +/- 10 -0.010743 0.5788926 -0.373539 0.3541955 0.3901666 SOX10 SRY (sex determining region Y)-box 10 1512 +/- 231 1765 +/- 300 1072 +/- 119 1226 +/- 105 -0.036581 0.5194585 0.357787 0.2775658 0.2698654 SOX11 SRY (sex determining region Y)-box 11 664 +/- 52 459 +/- 46 303 +/- 32 221 +/- 13 -9.38E-06 -0.014598 -0.048754 0.0117623 0.0555967 SOX8 SRY (sex determining region Y)-box 8 4560 +/- 534 5166 +/- 601 3026 +/- 177 3084 +/- 58 -0.000776 0.4684656 0.76591 0.184624 0.3038223 SPAG4 sperm associated antigen 4 127 +/- 23 141 +/- 18 93 +/- 13 97 +/- 6 -0.003243 0.6240954 0.756983 0.9201044 0.7807081 SPAG7 sperm associated antigen 7 821 +/- 34 775 +/- 17 722 +/- 42 731 +/- 25 -0.01507 -0.268687 0.86817 0.2739969 0.7802959 SPAG9 sperm associated antigen 9 477 +/- 60 705 +/- 50 407 +/- 40 498 +/- 44 -0.007115 0.0161276 0.158938 0.0151246 0.0335196 SPARC secreted protein, acidic, cysteine-rich (osteonectin) 8905 +/- 463 8242 +/- 567 7668 +/- 633 7800 +/- 553 -0.024834 -0.387006 0.8783 0.0628968 0.6293729 SPARCL1 SPARC-like 1 (mast9, hevin) 20455 +/- 810 21245 +/- 1040 19170 +/- 136 20139 +/- 549 -0.025195 0.5630312 0.154543 0.8203254 0.2140363

S2-genes differentially expressed across region.xls Page 69 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI SPCS1 signal peptidase complex subunit 1 homolog (S. cerevisiae) 6159 +/- 178 6206 +/- 222 5341 +/- 222 6203 +/- 226 -0.008114 0.8729474 0.023845 0.565696 0.2051347 SPFH2 SPFH domain family, member 2 1304 +/- 91 1526 +/- 47 1181 +/- 79 1298 +/- 53 -0.04899 0.0630554 0.248438 0.0451804 0.4763109 SPG21 spastic paraplegia 21 (autosomal recessive, Mast syndrome) 2258 +/- 168 2325 +/- 93 1675 +/- 47 1917 +/- 25 -0.000469 0.7353056 0.002344 0.9724206 0.0643185 SPIN1 spinster 704 +/- 30 692 +/- 15 625 +/- 19 591 +/- 17 -0.001482 -0.727428 -0.215797 0.995689 0.1087393 SPON1 spondin 1, extracellular matrix protein 3932 +/- 216 3444 +/- 271 2878 +/- 136 2785 +/- 177 -0.000402 -0.190823 -0.689139 0.0690268 0.2612625 SPOP speckle-type POZ protein 592 +/- 40 555 +/- 12 517 +/- 14 508 +/- 19 -0.011957 -0.414847 -0.722522 0.618913 0.7034069 SPRY4 sprouty homolog 4 (Drosophila) 610 +/- 57 510 +/- 59 368 +/- 27 339 +/- 21 -0.000288 -0.249621 -0.427974 0.2459985 0.7111496 SPSB1 splA/ryanodine receptor domain and SOCS box containing 1 459 +/- 70 573 +/- 96 232 +/- 15 269 +/- 25 -0.001037 0.3611914 0.257303 0.3080036 0.2377916 SPTLC1 serine palmitoyltransferase, long chain base subunit 1 2170 +/- 85 2440 +/- 104 1815 +/- 58 2111 +/- 70 -0.000449 0.0739814 0.010986 0.0123306 0.0526412 SPTLC2 serine palmitoyltransferase, long chain base subunit 2 254 +/- 29 289 +/- 42 162 +/- 20 230 +/- 28 -0.007986 0.5073701 0.088952 0.3263208 0.2920906 SQRDL sulfide quinone reductase-like (yeast) 544 +/- 35 567 +/- 50 379 +/- 39 373 +/- 14 -0.000394 0.7194467 -0.895267 0.9785462 0.5774806 SRA1 steroid receptor RNA activator 1 1111 +/- 39 1011 +/- 42 978 +/- 40 910 +/- 29 -0.012648 -0.113964 -0.202639 0.214092 0.719729 SREBF1 sterol regulatory element binding transcription factor 1 2104 +/- 259 2329 +/- 174 1764 +/- 82 1893 +/- 76 -0.036204 0.489834 0.279554 0.6932232 0.6517139 SRGAP1 SLIT-ROBO Rho GTPase activating protein 1 181 +/- 23 118 +/- 19 124 +/- 13 113 +/- 17 -0.028159 -0.060887 -0.612146 0.3001269 0.8563141 SRL sarcalumenin 199 +/- 11 222 +/- 15 157 +/- 11 153 +/- 14 -0.000216 0.2497416 -0.830879 0.1203476 0.6111236 SRP72 signal recognition particle 72kDa 2192 +/- 106 2030 +/- 131 2025 +/- 105 1927 +/- 137 -0.009117 -0.360844 -0.583591 0.9166134 0.8367181 SRPK1 SFRS protein kinase 1 1817 +/- 59 1693 +/- 67 1666 +/- 51 1568 +/- 49 -0.011348 -0.193987 -0.196262 0.1972328 0.1463466 SRPR signal recognition particle receptor ('docking protein') 1844 +/- 37 1767 +/- 54 1683 +/- 24 1662 +/- 48 -0.000453 -0.263969 -0.721713 0.1509418 0.2228499 SSBP2 Single-stranded DNA binding protein 2 148 +/- 42 473 +/- 63 131 +/- 36 339 +/- 32 -0.014338 0.0021597 0.00188 0.0049527 0.0027213 SSFA2 sperm specific antigen 2 1940 +/- 120 2218 +/- 179 1740 +/- 78 1949 +/- 103 -0.038664 0.2304902 0.144994 0.094111 0.3561498 SSH2 slingshot homolog 2 (Drosophila) 1235 +/- 80 1447 +/- 79 906 +/- 29 970 +/- 16 -0.000163 0.0884523 0.089311 0.0592516 0.1922352 SSR2 signal sequence receptor, beta (translocon-associated protein beta) 2068 +/- 137 1585 +/- 78 1623 +/- 93 1483 +/- 68 -0.002111 -0.01556 -0.256528 0.0048081 0.1498578 SSTR2 somatostatin receptor 2 181 +/- 26 94 +/- 7 135 +/- 18 66 +/- 7 -0.029494 -0.018012 -0.010197 0.0054803 0.0554253 SSU72 SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae) 2015 +/- 93 2625 +/- 115 1716 +/- 93 2050 +/- 54 -0.001308 0.0022663 0.015168 0.1119429 0.0672956 ST3GAL1 ST3 beta-galactoside alpha-2,3-sialyltransferase 1 171 +/- 22 151 +/- 13 123 +/- 12 105 +/- 10 -0.005419 -0.445923 -0.275251 0.3136454 0.1756918 ST3GAL5 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 3502 +/- 17 3278 +/- 141 3218 +/- 158 3058 +/- 84 -0.037835 -0.174871 -0.396941 0.2127487 0.7734036 ST8SIA2 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 339 +/- 35 212 +/- 17 124 +/- 23 93 +/- 11 -6.27E-05 -0.013714 -0.261209 0.0369402 0.6086224 STAC SH3 and cysteine rich domain 182 +/- 43 116 +/- 21 79 +/- 12 55 +/- 14 -0.003166 -0.218293 -0.22891 0.3123598 0.1344703 STAC2 SH3 and cysteine rich domain 2 655 +/- 79 726 +/- 61 585 +/- 47 555 +/- 28 -0.014899 0.4969834 -0.601054 0.8221281 0.4048439 STAP2 signal-transducing adaptor protein-2 238 +/- 28 216 +/- 22 195 +/- 19 172 +/- 16 -0.0264 -0.554155 -0.394298 0.3342414 0.4568618 STARD3NLSTARD3 N-terminal like 1387 +/- 85 942 +/- 16 1203 +/- 86 834 +/- 52 -0.044391 -0.003026 -0.006158 0.0077875 0.0443081 STAT4 signal transducer and activator of transcription 4 499 +/- 51 426 +/- 51 244 +/- 24 260 +/- 16 -0.000459 -0.342349 0.581356 0.1921317 0.4811733 STAT5B signal transducer and activator of transcription 5B 521 +/- 41 647 +/- 33 390 +/- 30 402 +/- 19 -0.000162 0.0378386 0.730509 0.0959601 0.5692423 STATIP1 signal transducer and activator of transcription 3 interacting protein 1 192 +/- 9 200 +/- 11 175 +/- 7 172 +/- 5 -0.014039 0.5761851 -0.73477 0.0715561 0.5751141 STAU2 staufen, RNA binding protein, homolog 2 (Drosophila) 1544 +/- 68 1433 +/- 75 1177 +/- 94 1092 +/- 26 -0.000151 -0.299679 -0.419622 0.1505723 0.5924159 STK16 serine/threonine kinase 16 1003 +/- 75 934 +/- 33 792 +/- 43 811 +/- 28 -0.002567 -0.429383 0.715518 0.1620612 0.8047645 STK3 serine/threonine kinase 3 (STE20 homolog, yeast) 306 +/- 31 350 +/- 30 248 +/- 29 215 +/- 9 -0.004177 0.3258765 -0.319178 0.1444463 0.401753 STK33 serine/threonine kinase 33 108 +/- 13 123 +/- 10 84 +/- 9 90 +/- 10 -0.016125 0.3829272 0.639826 0.1991779 0.8902341 STK39 serine threonine kinase 39 (STE20/SPS1 homolog, yeast) 1847 +/- 187 1846 +/- 170 1220 +/- 71 1109 +/- 21 -0.000252 -0.999279 -0.184001 0.7830289 0.486802 STK40 serine/threonine kinase 40 854 +/- 48 733 +/- 38 741 +/- 24 677 +/- 27 -0.017463 -0.080274 -0.10879 0.0890168 0.4602821 STMN4 stathmin-like 4 6297 +/- 736 7226 +/- 726 4568 +/- 192 5124 +/- 228 -0.003472 0.3896738 0.097795 0.2333729 0.1045957 STRBP spermatid perinuclear RNA binding protein 926 +/- 84 754 +/- 29 785 +/- 30 663 +/- 42 -0.037528 -0.098304 -0.049348 0.2126192 0.2508432 STS steroid sulfatase (microsomal), arylsulfatase C, isozyme S 522 +/- 29 428 +/- 25 424 +/- 42 346 +/- 14 -0.021387 -0.033391 -0.124594 0.0267615 0.1852603 STT3A STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) 1088 +/- 54 1052 +/- 27 935 +/- 47 988 +/- 32 -0.000417 -0.563674 0.378733 0.7024884 0.6669422 STX12 syntaxin 12 3568 +/- 232 3187 +/- 252 2719 +/- 109 2730 +/- 137 -0.003187 -0.291729 0.951933 0.6026275 0.9246573

S2-genes differentially expressed across region.xls Page 70 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI STX3 syntaxin 3 659 +/- 36 631 +/- 34 561 +/- 32 501 +/- 27 -0.001138 -0.577329 -0.18856 0.3676607 0.5598432 STX4 syntaxin 4 970 +/- 42 831 +/- 53 729 +/- 27 675 +/- 33 -0.000123 -0.071429 -0.238852 0.3496419 0.8957216 STYK1 serine/threonine/tyrosine kinase 1 1663 +/- 206 1301 +/- 132 1062 +/- 95 973 +/- 54 -0.014018 -0.174975 -0.43961 0.182045 0.7661065 SUCLG1 succinate-CoA ligase, GDP-forming, alpha subunit 4090 +/- 140 3799 +/- 126 3291 +/- 62 3434 +/- 113 -0.000816 -0.153623 0.309941 0.0769856 0.6830995 SURB7 SRB7 suppressor of RNA polymerase B homolog (yeast) 1618 +/- 167 2473 +/- 241 1436 +/- 158 2200 +/- 169 -0.002882 0.0173654 0.00959 0.0264854 0.0236746 SURF1 surfeit 1 1906 +/- 52 2026 +/- 101 1667 +/- 86 1959 +/- 45 -0.001352 0.3240518 0.018135 0.7308047 0.0332671 SURF4 surfeit 4 844 +/- 29 730 +/- 19 751 +/- 33 661 +/- 21 -0.005552 -0.010483 -0.048623 0.1021851 0.242073 SURF5 surfeit 5 671 +/- 35 675 +/- 39 628 +/- 21 630 +/- 33 -0.019229 0.9353535 0.953853 0.4694861 0.6321273 SUSD3 sushi domain containing 3 133 +/- 11 158 +/- 15 100 +/- 8 115 +/- 15 -0.001208 0.1963738 0.440701 0.0574398 0.2270891 SUSD5 sushi domain containing 5 152 +/- 16 179 +/- 26 105 +/- 8 101 +/- 4 -0.004127 0.4100232 -0.696263 0.5696639 0.8611905 SYBL1 synaptobrevin-like 1 2257 +/- 120 2398 +/- 173 1670 +/- 98 1902 +/- 111 -0.001637 0.5221661 0.154011 0.3264443 0.3403463 SYNGR4 synaptogyrin 4 345 +/- 36 312 +/- 23 289 +/- 21 221 +/- 22 -0.008731 -0.459799 -0.054898 0.6978628 0.2609005 SYNJ2 synaptojanin 2 2864 +/- 243 3440 +/- 265 2046 +/- 167 2454 +/- 148 -0.001217 0.1405879 0.099777 0.0299966 0.0826187 SYNPO2L synaptopodin 2-like 274 +/- 17 355 +/- 22 261 +/- 23 265 +/- 16 -0.049675 0.0168779 0.89537 0.025081 0.8519871 SYPL1 synaptophysin-like 1 3583 +/- 248 4205 +/- 292 3124 +/- 126 3310 +/- 50 -0.01368 0.1367387 0.212215 0.0555698 0.3792294 SYT12 synaptotagmin XII 471 +/- 30 454 +/- 15 393 +/- 27 397 +/- 23 -5.47E-05 -0.624515 0.920903 0.5148692 0.8999209 SYTL2 synaptotagmin-like 2 300 +/- 78 429 +/- 112 148 +/- 20 94 +/- 10 -0.006273 0.368996 -0.048362 0.4373566 0.1563739 TAF12 TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa 416 +/- 22 447 +/- 37 368 +/- 21 391 +/- 23 -0.047488 0.4921011 0.483023 0.1033617 0.3812812 TAF6L TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65k 170 +/- 28 231 +/- 29 131 +/- 18 158 +/- 11 -0.047624 0.1559957 0.222118 0.1285349 0.4286729 TAF7 TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa 4918 +/- 290 4864 +/- 249 4071 +/- 222 4314 +/- 212 -0.000604 -0.88971 0.44943 0.5876385 0.7835555 TAGLN3 transgelin 3 8773 +/- 613 8568 +/- 590 7683 +/- 413 7384 +/- 319 -0.006774 -0.814278 -0.582208 0.3759476 0.7040371 TA-NFKBHT-cell activation NFKB-like protein 182 +/- 21 157 +/- 19 149 +/- 10 111 +/- 16 -0.029674 -0.401066 -0.0836 0.3718951 0.2907645 TAOK3 TAO kinase 3 1375 +/- 110 1436 +/- 84 1229 +/- 51 1163 +/- 64 -0.008837 0.6672215 -0.445797 0.6944222 0.8765451 TAPBPL TAP binding protein-like 238 +/- 24 255 +/- 19 179 +/- 19 203 +/- 33 -0.014745 0.5928527 0.535816 0.3622389 0.531331 TARDBP TAR DNA binding protein 6008 +/- 205 5570 +/- 111 4929 +/- 296 4904 +/- 111 -0.006981 -0.099176 -0.937517 0.0959205 0.9070113 TAX1BP1 Tax1 (human T-cell leukemia virus type I) binding protein 1 2220 +/- 188 2772 +/- 275 1866 +/- 123 2493 +/- 268 -0.003659 0.1320265 0.080473 0.8374351 0.5951673 TAX1BP3 Tax1 (human T-cell leukemia virus type I) binding protein 3 495 +/- 43 525 +/- 26 448 +/- 18 457 +/- 11 -0.047121 0.5539292 0.679951 0.6454881 0.6205134 TBC1D12 TBC1 domain family, member 12 234 +/- 17 302 +/- 27 195 +/- 16 232 +/- 18 -0.039962 0.0609842 0.148761 0.0670071 0.8452444 TBC1D14 TBC1 domain family, member 14 2125 +/- 84 1815 +/- 92 1795 +/- 52 1639 +/- 43 -0.001141 -0.031696 -0.047245 0.2222672 0.2088684 TBC1D17 TBC1 domain family, member 17 1262 +/- 103 1539 +/- 80 1047 +/- 42 1261 +/- 39 -0.007657 0.0615205 0.004927 0.0854102 0.0422091 TBC1D2 TBC1 domain family, member 2 166 +/- 23 136 +/- 14 98 +/- 11 126 +/- 12 -0.036608 -0.300716 0.119388 0.8193561 0.3301407 TBC1D22ATBC1 domain family, member 22A 694 +/- 57 800 +/- 64 615 +/- 56 657 +/- 64 -0.032761 0.241692 0.62996 0.3037399 0.6998126 TBC1D23 TBC1 domain family, member 23 1555 +/- 45 1653 +/- 46 1421 +/- 46 1451 +/- 35 -6.02E-05 0.1545906 0.60528 0.0545596 0.8447142 TBC1D7 TBC1 domain family, member 7 696 +/- 65 587 +/- 30 597 +/- 40 556 +/- 8 -0.011985 -0.168953 -0.350739 0.2128575 0.3705893 TBC1D9 TBC1 domain family, member 9 5875 +/- 79 5392 +/- 40 4902 +/- 118 5160 +/- 124 -0.000105 -0.000793 0.165632 0.0006887 0.6018428 TBL1XR1 transducin (beta)-like 1X-linked receptor 1 6054 +/- 153 5768 +/- 138 5573 +/- 194 5567 +/- 116 -0.044855 -0.19556 -0.97936 0.3577985 0.4670105 TCEB2 transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B) 9094 +/- 408 8720 +/- 388 7768 +/- 202 7944 +/- 151 -0.000905 -0.521378 0.504044 0.522319 0.4461638 TCERG1L transcription elongation regulator 1-like 650 +/- 61 622 +/- 60 279 +/- 31 220 +/- 30 -5.05E-07 -0.754584 -0.200959 0.4588186 0.662007 TCF12 transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) 3252 +/- 190 3452 +/- 144 2724 +/- 157 2755 +/- 41 -0.000681 0.4222045 0.856976 0.2320191 0.8009612 TCF7L2 transcription factor 7-like 2 (T-cell specific, HMG-box) 1721 +/- 95 2204 +/- 120 1530 +/- 109 1712 +/- 113 -0.004094 0.0109185 0.276317 0.0005919 0.481373 TETRAN tetracycline transporter-like protein 1502 +/- 79 1665 +/- 55 1324 +/- 116 1477 +/- 58 -0.017747 0.1251175 0.274168 0.170974 0.3951985 TEX2 testis expressed sequence 2 2858 +/- 124 2844 +/- 75 2621 +/- 70 2533 +/- 74 -0.029018 -0.929672 -0.412529 0.8184058 0.4540058 TF transferrin 7019 +/- 1463 9974 +/- 1622 4189 +/- 354 6128 +/- 572 -0.012445 0.2061852 0.024171 0.1130597 0.0107645 TFG TRK-fused gene 5280 +/- 199 4443 +/- 99 4815 +/- 94 4186 +/- 147 -0.008725 -0.006472 -0.008783 0.00884 0.2431836 TFPI tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) 202 +/- 14 337 +/- 20 145 +/- 11 265 +/- 18 -0.000333 0.0003604 0.001002 0.0062585 0.0073557

S2-genes differentially expressed across region.xls Page 71 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI TFPT TCF3 (E2A) fusion partner (in childhood Leukemia) 369 +/- 36 499 +/- 34 283 +/- 25 365 +/- 25 -0.000916 0.0243374 0.04248 0.0484472 0.0114551 TGFBI transforming growth factor, beta-induced, 68kDa 198 +/- 33 110 +/- 12 75 +/- 20 58 +/- 9 -0.01942 -0.067467 -0.466341 0.0597685 0.586412 TGFBR1 transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kDa) 1158 +/- 54 1250 +/- 84 1057 +/- 37 1019 +/- 39 -0.015771 0.3799338 -0.498251 0.0579136 0.7932237 TGIF TGFB-induced factor (TALE family homeobox) 415 +/- 35 516 +/- 83 285 +/- 28 358 +/- 24 -0.016122 0.2967985 0.075203 0.0427712 0.0772841 THOC5 THO complex 5 765 +/- 46 710 +/- 24 612 +/- 40 546 +/- 21 -6.06E-07 -0.313424 -0.181253 0.5831286 0.4904124 THSD1 thrombospondin, type I, domain containing 1 225 +/- 19 241 +/- 13 201 +/- 12 230 +/- 16 -0.024703 0.528681 0.179766 0.7732635 0.5771873 THUMPD2 THUMP domain containing 2 297 +/- 26 340 +/- 13 273 +/- 23 267 +/- 20 -0.031588 0.1817258 -0.841428 0.1403765 0.9750369 TIMM10 translocase of inner mitochondrial membrane 10 homolog (yeast) 1629 +/- 120 1047 +/- 77 1208 +/- 47 967 +/- 68 -0.01987 -0.003153 -0.021238 0.0054885 0.1440759 TIMM13 translocase of inner mitochondrial membrane 13 homolog (yeast) 2117 +/- 135 2107 +/- 61 1853 +/- 52 1848 +/- 82 -0.005034 -0.948966 -0.964 0.5007031 0.6750839 TM4SF18 Transmembrane 4 L six family member 18 153 +/- 13 142 +/- 18 132 +/- 13 99 +/- 14 -0.021488 -0.616619 -0.114351 0.9690264 0.2755219 TM6SF1 transmembrane 6 superfamily member 1 983 +/- 82 1128 +/- 151 621 +/- 46 758 +/- 56 -0.002232 0.4238201 0.093395 0.8476168 0.3604281 TM7SF2 transmembrane 7 superfamily member 2 1226 +/- 65 1455 +/- 59 1141 +/- 34 1258 +/- 73 -0.037433 0.0261398 0.201389 0.0392401 0.5412888 TMBIM1 transmembrane BAX inhibitor motif containing 1 2080 +/- 216 2514 +/- 203 1362 +/- 54 1608 +/- 56 -0.000612 0.1734264 0.012063 0.0932183 0.1379688 TMC6 transmembrane channel-like 6 289 +/- 73 451 +/- 67 233 +/- 44 287 +/- 28 -0.037 0.1341396 0.330175 0.0777583 0.23139 TMC7 transmembrane channel-like 7 489 +/- 91 732 +/- 166 265 +/- 40 317 +/- 24 -0.009612 0.2355944 0.299908 0.0671777 0.0984577 TMED10 transmembrane emp24-like trafficking protein 10 (yeast) 2096 +/- 68 2382 +/- 217 1504 +/- 99 1726 +/- 109 -0.001198 0.2557307 0.166505 0.1263812 0.4455591 TMED4 transmembrane emp24 protein transport domain containing 4 1134 +/- 55 1431 +/- 104 1087 +/- 50 1361 +/- 59 -0.045916 0.0364997 0.007201 0.0773 0.0247357 TMEFF1 transmembrane protein with EGF-like and two follistatin-like domains 1 2516 +/- 241 2897 +/- 346 2173 +/- 125 1935 +/- 86 -0.01485 0.3899269 -0.152192 0.1217905 0.5016522 TMEM10 transmembrane protein 10 3669 +/- 618 5332 +/- 1409 1617 +/- 278 2033 +/- 179 -0.005902 0.3161815 0.24258 0.0447156 0.0692211 TMEM100 transmembrane protein 100 1126 +/- 85 764 +/- 92 810 +/- 93 482 +/- 73 -8.87E-06 -0.016282 -0.021697 0.0020981 0.0122112 TMEM11 transmembrane protein 11 845 +/- 33 981 +/- 25 769 +/- 31 945 +/- 37 -0.003105 0.0089299 0.006195 0.1398978 0.1068125 TMEM111 transmembrane protein 111 1589 +/- 60 1468 +/- 57 1300 +/- 50 1373 +/- 62 -0.003408 -0.173349 0.384283 0.2954679 0.2599387 TMEM116 transmembrane protein 116 119 +/- 13 198 +/- 25 90 +/- 10 142 +/- 15 -0.009295 0.0227208 0.023266 0.0091373 0.0654129 TMEM117 transmembrane protein 117 156 +/- 17 204 +/- 36 131 +/- 14 163 +/- 23 -0.032149 0.2611902 0.282804 0.1336358 0.0299912 TMEM123 transmembrane protein 123 1006 +/- 86 1324 +/- 185 668 +/- 53 697 +/- 24 -0.001602 0.1612782 0.632965 0.020275 0.7832746 TMEM125 transmembrane protein 125 1877 +/- 348 2703 +/- 395 920 +/- 69 1320 +/- 139 -0.003931 0.1481536 0.042879 0.0392519 0.0982602 TMEM126Atransmembrane protein 126A 3507 +/- 125 3122 +/- 143 3119 +/- 73 3024 +/- 134 -0.027968 -0.069902 -0.555831 0.0561418 0.4291045 TMEM132Btransmembrane protein 132B 1274 +/- 123 930 +/- 71 1051 +/- 67 730 +/- 54 -0.001972 -0.041705 -0.004742 0.2404027 0.1566965 TMEM132Ctransmembrane protein 132C 685 +/- 65 666 +/- 31 466 +/- 32 418 +/- 32 -0.00016 -0.798761 -0.318844 0.6632678 0.6726583 TMEM134 transmembrane protein 134 869 +/- 72 1058 +/- 55 744 +/- 31 792 +/- 58 -0.002805 0.0660617 0.490246 0.0720144 0.2240706 TMEM141 transmembrane protein 141 1178 +/- 93 1179 +/- 170 822 +/- 36 803 +/- 73 -0.001507 0.9947763 -0.823851 0.4254468 0.2983975 TMEM144 transmembrane protein 144 2319 +/- 434 3785 +/- 835 1023 +/- 194 1521 +/- 159 -0.004677 0.1600789 0.078679 0.049251 0.1244338 TMEM14A transmembrane protein 14A 5032 +/- 299 5119 +/- 141 4337 +/- 262 4685 +/- 423 -0.002904 0.7990582 0.507207 0.8458168 0.4591046 TMEM16D transmembrane protein 16D 287 +/- 24 220 +/- 14 135 +/- 14 141 +/- 14 -2.12E-05 -0.043747 0.778349 0.0263544 0.8800053 TMEM16K transmembrane protein 16K 684 +/- 69 633 +/- 20 439 +/- 19 451 +/- 16 -0.000255 -0.506042 0.633738 0.2597513 0.2615007 TMEM23 transmembrane protein 23 637 +/- 52 624 +/- 26 513 +/- 52 478 +/- 15 -0.010821 -0.827126 -0.537566 0.6479802 0.4806171 TMEM31 transmembrane protein 31 169 +/- 17 201 +/- 30 131 +/- 9 124 +/- 10 -0.012336 0.3774421 -0.630391 0.1461281 0.7377884 TMEM32 transmembrane protein 32 1686 +/- 64 1704 +/- 35 1536 +/- 43 1602 +/- 60 -0.01792 0.8108688 0.400605 0.5529983 0.8330296 TMEM41A Transmembrane protein 41A 242 +/- 11 261 +/- 18 213 +/- 6 231 +/- 9 -0.019711 0.3929711 0.155152 0.2823279 0.0867396 TMEM42 transmembrane protein 42 1365 +/- 45 1466 +/- 33 1244 +/- 38 1458 +/- 62 -0.040193 0.1013395 0.022844 0.3727597 0.1865494 TMEM49 transmembrane protein 49 2310 +/- 181 2124 +/- 109 1915 +/- 64 2005 +/- 98 -0.015649 -0.402536 0.463548 0.109979 0.9583833 TMEM5 transmembrane protein 5 1006 +/- 53 1106 +/- 58 882 +/- 59 912 +/- 32 -0.000983 0.2340156 0.669264 0.3283794 0.9878123 TMEM50B transmembrane protein 50B 10086 +/- 744 7418 +/- 951 7691 +/- 435 6249 +/- 882 -0.010509 -0.053133 -0.193944 0.0045491 0.0299147 TMEM51 transmembrane protein 51 459 +/- 34 555 +/- 15 354 +/- 21 396 +/- 28 -0.000141 0.0384605 0.277148 0.1356155 0.5956454 TMEM62 transmembrane protein 62 272 +/- 20 338 +/- 21 254 +/- 9 273 +/- 20 -0.024941 0.0504131 0.433539 0.0665573 0.7988088

S2-genes differentially expressed across region.xls Page 72 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI TMEM77 transmembrane protein 77 1008 +/- 35 1082 +/- 86 902 +/- 22 909 +/- 42 -0.008777 0.4508496 0.894352 0.0741051 0.2304578 TMEM80 transmembrane protein 80 995 +/- 50 1270 +/- 42 785 +/- 32 942 +/- 27 -1.15E-05 0.0019743 0.00455 0.0025206 0.0233829 TMEM91 transmembrane protein 91 837 +/- 63 517 +/- 43 501 +/- 26 438 +/- 36 -0.003916 -0.00249 -0.196617 0.0104634 0.54782 TMEM95 transmembrane protein 95 431 +/- 78 521 +/- 65 295 +/- 13 314 +/- 39 -0.01145 0.3948066 0.668059 0.3866796 0.6229672 TMEM97 transmembrane protein 97 1042 +/- 36 1322 +/- 88 914 +/- 45 1135 +/- 30 -0.00711 0.0231497 0.003205 0.0174164 0.0196386 TMEM98 transmembrane protein 98 1104 +/- 116 1292 +/- 184 606 +/- 45 748 +/- 70 -0.001678 0.4120835 0.133539 0.072057 0.2145276 TMEM99 transmembrane protein 99 1082 +/- 48 1094 +/- 40 875 +/- 47 942 +/- 20 -1.72E-05 0.855035 0.230958 0.9665876 0.5153736 TMSB10 thymosin, beta 10 19055 +/- 1135 17143 +/- 1661 14060 +/- 1235 13713 +/- 1031 -0.000416 -0.367193 -0.834313 0.1867661 0.5824763 TMTC2 transmembrane and tetratricopeptide repeat containing 2 1180 +/- 126 1475 +/- 241 798 +/- 46 811 +/- 49 -0.007281 0.3120659 0.85678 0.1139305 0.8947591 TMTC4 transmembrane and tetratricopeptide repeat containing 4 1389 +/- 212 2022 +/- 276 751 +/- 81 984 +/- 63 -0.000888 0.1009476 0.049565 0.1159551 0.1834717 TNFAIP1 tumor necrosis factor, alpha-induced protein 1 (endothelial) 1266 +/- 56 1364 +/- 79 1097 +/- 69 1144 +/- 42 -0.004513 0.3408433 0.584352 0.7164751 0.8064452 TNFAIP6 tumor necrosis factor, alpha-induced protein 6 258 +/- 31 467 +/- 111 161 +/- 13 251 +/- 29 -0.02623 0.1228848 0.031792 0.012802 0.0430429 TNFRSF1Atumor necrosis factor receptor superfamily, member 1A 690 +/- 39 724 +/- 15 639 +/- 33 674 +/- 26 -0.032037 0.4472086 0.410385 0.9337673 0.9435001 TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 626 +/- 50 900 +/- 75 508 +/- 50 663 +/- 50 -0.023771 0.0144421 0.056884 0.006527 0.1341425 TNIP3 TNFAIP3 interacting protein 3 241 +/- 75 259 +/- 63 129 +/- 12 129 +/- 24 -0.017255 0.8575564 0.994032 0.7621026 0.2182326 TNNT1 troponin T type 1 (skeletal, slow) 480 +/- 67 379 +/- 49 279 +/- 10 282 +/- 13 -0.004255 -0.256996 0.888727 0.1533947 0.7468215 TNRC8 trinucleotide repeat containing 8 1197 +/- 27 1075 +/- 90 1008 +/- 34 991 +/- 92 -0.026475 -0.239098 -0.869522 0.0416345 0.1696799 TNS3 tensin 3 5926 +/- 520 7061 +/- 361 4964 +/- 174 6047 +/- 403 -0.002609 0.1067943 0.052473 0.3937513 0.3502093 TNXA tenascin XA pseudogene 497 +/- 80 915 +/- 160 295 +/- 39 476 +/- 26 -0.012749 0.0506152 0.004207 0.0098381 0.0079701 TOMM7 translocase of outer mitochondrial membrane 7 homolog (yeast) 17105 +/- 620 17063 +/- 697 14743 +/- 300 16384 +/- 704 -0.031531 -0.965458 0.080481 0.955107 0.0384624 TOX thymus high mobility group box protein TOX 193 +/- 43 267 +/- 53 97 +/- 33 86 +/- 18 -0.002 0.3044172 -0.781925 0.4045686 0.9984712 TP53I3 tumor protein p53 inducible protein 3 233 +/- 21 323 +/- 39 196 +/- 27 222 +/- 19 -0.005994 0.0761319 0.457613 0.371556 0.9136729 TP53INP2 tumor protein p53 inducible nuclear protein 2 4502 +/- 647 5735 +/- 906 2898 +/- 254 3656 +/- 275 -0.015201 0.2963812 0.074433 0.0962711 0.0598894 TPBG trophoblast glycoprotein 425 +/- 74 290 +/- 22 280 +/- 32 252 +/- 29 -0.046539 -0.130069 -0.532739 0.187662 0.73036 TPD52L1 tumor protein D52-like 1 1678 +/- 239 1486 +/- 175 1145 +/- 36 1308 +/- 167 -0.024544 -0.533223 0.390696 0.8205144 0.1585684 TPD52L2 tumor protein D52-like 2 2130 +/- 101 2242 +/- 40 1785 +/- 74 1860 +/- 60 -0.001347 0.3376215 0.453274 0.3109445 0.4664876 TPRKB TP53RK binding protein 296 +/- 6 269 +/- 11 275 +/- 21 229 +/- 14 -0.04136 -0.05871 -0.100795 0.0451972 0.4756008 TPST2 tyrosylprotein sulfotransferase 2 1104 +/- 39 1008 +/- 50 1000 +/- 42 925 +/- 54 -0.018596 -0.16616 -0.307659 0.3258407 0.3255292 TRAF5 TNF receptor-associated factor 5 706 +/- 45 663 +/- 42 592 +/- 28 554 +/- 14 -0.036503 -0.49623 -0.269719 0.440349 0.8873457 TRAFD1 TRAF-type zinc finger domain containing 1 1185 +/- 77 1021 +/- 41 913 +/- 16 812 +/- 32 -0.000443 -0.098765 -0.028756 0.2705776 0.0476544 TRAK2 trafficking protein, kinesin binding 2 2695 +/- 155 3432 +/- 218 2515 +/- 153 2941 +/- 67 -0.029831 0.0224005 0.039325 0.0113992 0.044261 TRAM1 translocation associated membrane protein 1 2897 +/- 113 3090 +/- 156 2217 +/- 59 2441 +/- 84 -8.54E-05 0.3432087 0.063704 0.1270406 0.1480807 TRAM1L1 translocation associated membrane protein 1-like 1 638 +/- 47 545 +/- 37 569 +/- 36 527 +/- 17 -0.049402 -0.154149 -0.325713 0.3797812 0.6716263 TRAPPC3 trafficking protein particle complex 3 2640 +/- 113 2794 +/- 149 2242 +/- 126 2315 +/- 154 -0.002761 0.429548 0.720252 0.50484 0.5742297 TRAPPC4 trafficking protein particle complex 4 2721 +/- 85 2573 +/- 45 2301 +/- 33 2311 +/- 111 -0.000458 -0.161868 0.936534 0.2485648 0.8590408 TREML1 triggering receptor expressed on myeloid cells-like 1 204 +/- 19 240 +/- 14 170 +/- 10 186 +/- 17 -0.011396 0.1658589 0.443808 0.0938203 0.7478014 TRIM22 tripartite motif-containing 22 782 +/- 58 916 +/- 73 631 +/- 35 721 +/- 51 -0.002919 0.1823005 0.186117 0.0369424 0.0455313 TRIM26 tripartite motif-containing 26 633 +/- 65 690 +/- 74 540 +/- 34 483 +/- 19 -0.020513 0.5745436 -0.191967 0.3058803 0.2343582 TRIM45 tripartite motif-containing 45 174 +/- 14 187 +/- 15 127 +/- 7 171 +/- 24 -0.007586 0.5376546 0.141252 0.9981157 0.6189933 TRIM62 tripartite motif-containing 62 489 +/- 22 467 +/- 74 386 +/- 26 340 +/- 23 -0.013178 -0.781925 -0.215636 0.5740182 0.4832981 TRIM66 tripartite motif-containing 66 399 +/- 62 451 +/- 53 306 +/- 13 323 +/- 17 -0.008839 0.5397541 0.449346 0.9670394 0.9394052 TRIM8 tripartite motif-containing 8 2072 +/- 134 1995 +/- 107 1703 +/- 42 1583 +/- 45 -0.001021 -0.663793 -0.083724 0.5942171 0.0992646 TRIP6 thyroid hormone receptor interactor 6 448 +/- 39 566 +/- 21 435 +/- 17 459 +/- 14 -0.045033 0.0308227 0.305349 0.1291424 0.8550607 TSEN34 tRNA splicing endonuclease 34 homolog (S. cerevisiae) 991 +/- 52 1198 +/- 77 784 +/- 49 985 +/- 39 -0.00045 0.0529505 0.010934 0.3530183 0.0224772 TSGA10 testis specific, 10 320 +/- 34 273 +/- 35 216 +/- 16 205 +/- 6 -0.003369 -0.355906 -0.56521 0.6339427 0.9859942

S2-genes differentially expressed across region.xls Page 73 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI TSHZ2 Teashirt family zinc finger 2 295 +/- 104 395 +/- 118 168 +/- 50 148 +/- 28 -0.016645 0.5416636 -0.723938 0.8433482 0.9433977 TSPAN5 tetraspanin 5 7109 +/- 792 6252 +/- 373 3724 +/- 226 4003 +/- 207 -0.000629 -0.359568 0.386669 0.3452767 0.5477652 TTBK2 Tau tubulin kinase 2 1287 +/- 128 1604 +/- 88 1035 +/- 99 1388 +/- 79 -8.85E-05 0.0719335 0.02098 0.0493838 0.0120741 TTC1 tetratricopeptide repeat domain 1 3073 +/- 161 3032 +/- 98 2309 +/- 92 2550 +/- 73 -5.53E-05 -0.835261 0.071231 0.6220168 0.2547885 TTC23 tetratricopeptide repeat domain 23 257 +/- 18 216 +/- 9 216 +/- 15 210 +/- 19 -0.021089 -0.079969 -0.830796 0.0918539 0.4283755 TTL tubulin tyrosine ligase 814 +/- 54 875 +/- 68 691 +/- 38 714 +/- 29 -0.00844 0.5024002 0.639894 0.4407879 0.7570256 TTLL4 tubulin tyrosine ligase-like family, member 4 380 +/- 22 458 +/- 23 287 +/- 14 309 +/- 11 -0.000194 0.0373522 0.234891 0.0031157 0.3031171 TUBB2B tubulin, beta 2B 3672 +/- 237 3414 +/- 308 3355 +/- 175 2679 +/- 191 -0.021638 -0.521948 -0.029004 0.7455158 0.0914478 TUBB2C tubulin, beta 2C 6113 +/- 306 5607 +/- 320 5167 +/- 184 4784 +/- 104 -0.005635 -0.28004 -0.109006 0.4483524 0.350733 TUBB6 tubulin, beta 6 258 +/- 62 139 +/- 14 221 +/- 66 111 +/- 10 -0.024856 -0.113924 -0.152978 0.0931711 0.2290146 TUBGCP3 tubulin, gamma complex associated protein 3 912 +/- 58 972 +/- 37 839 +/- 21 801 +/- 22 -0.00633 0.4046451 -0.247261 0.2318623 0.6549196 TUFT1 tuftelin 1 796 +/- 100 1318 +/- 115 650 +/- 8 921 +/- 37 -0.01834 0.0067359 0.001405 0.0112191 0.03166 TUG1 taurine upregulated gene 1 4033 +/- 292 4761 +/- 163 3178 +/- 178 3449 +/- 128 -1.29E-05 0.0617943 0.247537 0.0605662 0.3851874 TXNDC11 thioredoxin domain containing 11 412 +/- 9 411 +/- 25 352 +/- 31 366 +/- 15 -0.019628 -0.986322 0.70255 0.4615681 0.908829 TXNDC4 thioredoxin domain containing 4 (endoplasmic reticulum) 1246 +/- 67 1050 +/- 62 1153 +/- 88 920 +/- 29 -0.037892 -0.057809 -0.044765 0.3896292 0.2612776 TXNDC5 thioredoxin domain containing 5 1374 +/- 68 1288 +/- 61 1186 +/- 38 1210 +/- 40 -0.010084 -0.37212 0.675468 0.1387274 0.6543168 TXNIP thioredoxin interacting protein 5289 +/- 536 5717 +/- 204 3799 +/- 467 4165 +/- 165 -2.27E-05 0.4813546 0.486203 0.5074603 0.8279049 TYMS thymidylate synthetase 239 +/- 53 386 +/- 88 103 +/- 7 138 +/- 26 -0.011587 0.1915353 0.255007 0.0685544 0.8354915 TYROBP TYRO protein tyrosine kinase binding protein 1055 +/- 57 1424 +/- 146 717 +/- 24 899 +/- 63 -0.00327 0.0533008 0.041326 0.0060139 0.1496422 U2AF1L2 U2 small nuclear RNA auxiliary factor 1-like 2 387 +/- 36 332 +/- 19 298 +/- 20 292 +/- 23 -0.005164 -0.21513 -0.862584 0.4774695 0.7373872 UBA52 ubiquitin A-52 residue ribosomal protein fusion product 1 10074 +/- 469 10094 +/- 459 7804 +/- 222 7562 +/- 358 -1.15E-05 0.9764199 -0.583322 0.7630909 0.6502829 UBAP2L ubiquitin associated protein 2-like 1045 +/- 48 1221 +/- 34 950 +/- 19 1068 +/- 43 -0.002699 0.0145306 0.047736 0.033027 0.2012221 UBE1L ubiquitin-activating enzyme E1-like 415 +/- 26 418 +/- 20 349 +/- 22 313 +/- 14 -0.00034 0.9134008 -0.198316 0.6839851 0.2172874 UBE2G1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 643 +/- 51 771 +/- 63 502 +/- 12 695 +/- 17 -0.021358 0.150032 2.34E-05 0.3163649 0.0032084 UBE2V1 ubiquitin-conjugating enzyme E2 variant 1 5462 +/- 163 4988 +/- 212 4639 +/- 86 4820 +/- 111 -0.010593 -0.108694 0.233362 0.074307 0.581624 UBE3A ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syn 6737 +/- 339 5705 +/- 371 5837 +/- 154 5224 +/- 81 -0.046238 -0.067394 -0.008728 0.1696783 0.0658193 UBE4A ubiquitination factor E4A (UFD2 homolog, yeast) 3607 +/- 89 3639 +/- 134 3135 +/- 59 3280 +/- 92 -0.002075 0.845674 0.228291 0.7030251 0.5943389 UBL7 ubiquitin-like 7 (bone marrow stromal cell-derived) 1773 +/- 45 1687 +/- 70 1665 +/- 96 1631 +/- 70 -0.027501 -0.328403 -0.779048 0.058029 0.4958572 UBOX5 U-box domain containing 5 532 +/- 29 614 +/- 41 430 +/- 21 481 +/- 13 -0.000849 0.1380396 0.070685 0.0691841 0.0095445 UBP1 upstream binding protein 1 (LBP-1a) 5553 +/- 347 5901 +/- 351 4548 +/- 161 4656 +/- 108 -0.002056 0.4959916 0.592553 0.1544972 0.2652676 UBQLN4 ubiquilin 4 2237 +/- 158 2044 +/- 65 1764 +/- 124 1670 +/- 82 -0.000358 -0.297369 -0.543512 0.3034843 0.9766542 UCK1 uridine-cytidine kinase 1 1401 +/- 163 1218 +/- 106 1046 +/- 54 836 +/- 69 -0.004824 -0.371207 -0.04278 0.4779683 0.0327209 UFC1 ubiquitin-fold modifier conjugating enzyme 1 3552 +/- 80 3489 +/- 180 3025 +/- 89 3035 +/- 152 -0.001756 -0.760268 0.95442 0.7869309 0.1523464 UNC50 unc-50 homolog (C. elegans) 2544 +/- 110 2493 +/- 78 2077 +/- 115 2257 +/- 132 -0.000436 -0.713524 0.334193 0.448593 0.8387599 UNC5B unc-5 homolog B (C. elegans) 1063 +/- 172 1437 +/- 225 776 +/- 87 783 +/- 33 -0.014097 0.2183388 0.946934 0.0788974 0.9331042 UNQ338 LGLL338 161 +/- 17 147 +/- 13 122 +/- 13 125 +/- 22 -0.049749 -0.544891 0.895831 0.5642408 0.2403051 UQCRQ ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa 9854 +/- 493 9051 +/- 586 7551 +/- 253 7899 +/- 364 -0.002507 -0.319624 0.456759 0.2468119 0.3634864 UROD uroporphyrinogen decarboxylase 853 +/- 28 837 +/- 32 731 +/- 30 753 +/- 17 -6.12E-06 -0.703895 0.54355 0.5836733 0.3061872 USMG5 upregulated during skeletal muscle growth 5 homolog (mouse) 6103 +/- 231 5953 +/- 295 5136 +/- 309 5013 +/- 98 -0.004019 -0.698373 -0.718204 0.5407318 0.9485892 USP16 ubiquitin specific peptidase 16 2644 +/- 41 2740 +/- 45 2363 +/- 11 2432 +/- 39 -2.1E-05 0.1466757 0.155418 0.1155372 0.4144073 USP24 ubiquitin specific peptidase 24 1423 +/- 41 1339 +/- 35 1236 +/- 71 1134 +/- 33 -0.000486 -0.15288 -0.235263 0.0665959 0.1237188 USP25 ubiquitin specific peptidase 25 1181 +/- 100 1073 +/- 45 895 +/- 37 944 +/- 33 -0.007661 -0.357436 0.342985 0.2096952 0.1333635 USP30 Ubiquitin specific peptidase 30 1266 +/- 52 1470 +/- 81 1076 +/- 43 1124 +/- 27 -0.000896 0.06356 0.374687 0.0663158 0.3657258 USP35 ubiquitin specific peptidase 35 723 +/- 79 654 +/- 78 550 +/- 28 547 +/- 89 -0.004771 -0.548233 -0.980309 0.2181016 0.3237613 USP40 Ubiquitin specific peptidase 40 406 +/- 43 522 +/- 47 286 +/- 12 340 +/- 21 -0.001279 0.0982792 0.060868 0.106011 0.3948635

S2-genes differentially expressed across region.xls Page 74 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI USP46 ubiquitin specific peptidase 46 4376 +/- 301 3749 +/- 289 3148 +/- 96 3106 +/- 71 -0.001148 -0.164097 -0.735468 0.3955782 0.9640516 USP9X ubiquitin specific peptidase 9, X-linked 1393 +/- 46 1365 +/- 39 1118 +/- 44 1092 +/- 22 -3.98E-05 -0.648364 -0.612075 0.6927944 0.5020402 UTRN utrophin (homologous to dystrophin) 778 +/- 80 836 +/- 96 535 +/- 37 528 +/- 26 -9.39E-05 0.6497611 -0.874117 0.8270791 0.517505 UVRAG UV radiation resistance associated gene 689 +/- 38 661 +/- 28 603 +/- 8 596 +/- 17 -0.003463 -0.56975 -0.742658 0.8084487 0.8823588 VAPA VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa 11082 +/- 381 9798 +/- 151 10132 +/- 197 8992 +/- 317 -0.01107 -0.018084 -0.018774 0.1149009 0.004102 VARSL valyl-tRNA synthetase like 979 +/- 65 917 +/- 38 794 +/- 39 773 +/- 44 -8.67E-05 -0.437222 -0.736868 0.7868086 0.8304472 VAV3 vav 3 oncogene 260 +/- 51 241 +/- 33 180 +/- 15 148 +/- 16 -0.020055 -0.766793 -0.187852 0.3567513 0.1630713 VCP valosin-containing protein 1102 +/- 39 1144 +/- 45 966 +/- 36 1049 +/- 44 -0.01094 0.4980118 0.1826 0.5609657 0.1624896 VDAC1 voltage-dependent anion channel 1 10509 +/- 219 9821 +/- 619 8704 +/- 233 9278 +/- 468 -0.002867 -0.333655 0.314765 0.0726371 0.8313087 VDAC2 voltage-dependent anion channel 2 7034 +/- 244 6827 +/- 253 6349 +/- 197 6691 +/- 271 -0.013633 -0.569095 0.338771 0.181822 0.7730682 VGLL4 vestigial like 4 (Drosophila) 1518 +/- 61 1412 +/- 70 1161 +/- 30 1109 +/- 28 -7.3E-06 -0.278628 -0.235746 0.6525835 0.5326966 VLDLR very low density lipoprotein receptor 1209 +/- 73 1269 +/- 119 837 +/- 53 801 +/- 52 -0.00028 0.6795589 -0.646197 0.4324676 0.6755647 VMO1 vitelline membrane outer layer 1 homolog (chicken) 691 +/- 50 1261 +/- 202 543 +/- 79 843 +/- 117 -0.002328 0.0360849 0.069831 0.0180857 0.022593 VPS11 vacuolar protein sorting 11 (yeast) 1606 +/- 43 1717 +/- 54 1384 +/- 37 1499 +/- 37 -1.44E-05 0.1392299 0.056107 0.6825803 0.3694175 VPS25 vacuolar protein sorting 25 homolog (S. cerevisiae) 1301 +/- 19 1308 +/- 32 1166 +/- 20 1194 +/- 29 -5.53E-05 0.8506718 0.464655 0.8720726 0.839856 VPS26A vacuolar protein sorting 26 homolog A (yeast) 4021 +/- 256 4122 +/- 132 3598 +/- 103 3581 +/- 174 -0.001178 0.7367235 -0.934864 0.8993346 0.8660691 VPS4B vacuolar protein sorting 4 homolog B (S. cerevisiae) 1185 +/- 93 1276 +/- 45 1112 +/- 58 1113 +/- 43 -0.007193 0.4046993 0.98919 0.1550663 0.4698651 VWA1 von Willebrand factor A domain containing 1 657 +/- 93 816 +/- 108 494 +/- 41 588 +/- 30 -0.021513 0.2897269 0.095404 0.2826215 0.0372321 WAC WW domain containing adaptor with coiled-coil 3946 +/- 99 4045 +/- 77 3700 +/- 73 4022 +/- 31 -0.008381 0.4501341 0.005534 0.3428936 0.03231 WARS tryptophanyl-tRNA synthetase 1334 +/- 60 1422 +/- 80 1043 +/- 43 1162 +/- 34 -1.83E-05 0.398165 0.059022 0.7965628 0.1886048 WASPIP Wiskott-Aldrich syndrome protein interacting protein 453 +/- 53 836 +/- 159 339 +/- 48 483 +/- 31 -0.031369 0.0615464 0.036627 0.0056073 0.0362281 WDFY1 WD repeat and FYVE domain containing 1 1082 +/- 175 808 +/- 190 857 +/- 69 579 +/- 68 -0.015588 -0.316252 -0.018632 0.8156256 0.2181605 WDR13 WD repeat domain 13 912 +/- 48 932 +/- 53 761 +/- 28 826 +/- 29 -0.019993 0.7807711 0.140289 0.8884983 0.0187858 WDR16 WD repeat domain 16 352 +/- 58 263 +/- 42 200 +/- 26 166 +/- 33 -0.030829 -0.240965 -0.440948 0.255448 0.463629 WDR18 WD repeat domain 18 291 +/- 13 310 +/- 30 259 +/- 18 250 +/- 8 -0.033086 0.5805064 -0.640415 0.2445521 0.8073076 WDR20 WD repeat domain 20 126 +/- 15 179 +/- 17 107 +/- 16 129 +/- 10 -0.02678 0.039402 0.281023 0.0059351 0.4156628 WDR24 WD repeat domain 24 361 +/- 20 312 +/- 15 308 +/- 8 282 +/- 18 -0.001215 -0.075995 -0.24858 0.0422095 0.0070221 WDR26 WD repeat domain 26 1894 +/- 85 1730 +/- 89 1521 +/- 83 1535 +/- 51 -0.000592 -0.214324 0.890893 0.5491414 0.9420649 WDR51B WD repeat domain 51B 654 +/- 107 685 +/- 65 551 +/- 48 463 +/- 25 -0.02057 0.809955 -0.138971 0.5816266 0.4971565 WDR54 WD repeat domain 54 1339 +/- 70 1280 +/- 65 1149 +/- 26 1125 +/- 59 -0.003338 -0.552485 -0.727311 0.7934557 0.9598929 WDR71 WD repeat domain 71 1094 +/- 63 1355 +/- 56 978 +/- 43 1086 +/- 116 -0.007047 0.0110987 0.422752 0.0963653 0.7656435 WDR74 WD repeat domain 74 744 +/- 14 687 +/- 26 664 +/- 37 566 +/- 24 -0.004433 -0.095725 -0.05306 0.2019727 0.1043173 WIBG within bgcn homolog (Drosophila) 453 +/- 36 532 +/- 22 420 +/- 46 462 +/- 43 -0.019784 0.0950643 0.515238 0.3446929 0.9162314 WIPI2 WD repeat domain, phosphoinositide interacting 2 4733 +/- 242 4270 +/- 138 3716 +/- 142 3609 +/- 94 -0.000211 -0.134098 -0.547963 0.1833678 0.9265282 WNK1 WNK lysine deficient protein kinase 1 169 +/- 12 233 +/- 22 147 +/- 11 191 +/- 17 -0.025793 0.0311083 0.069392 0.0020538 0.0029629 WWC1 WW, C2 and coiled-coil domain containing 1 1143 +/- 78 964 +/- 43 793 +/- 33 836 +/- 38 -0.003458 -0.082054 0.42034 0.0337062 0.6989543 WWOX WW domain containing oxidoreductase 524 +/- 32 696 +/- 66 457 +/- 17 483 +/- 57 -0.018561 0.0511504 0.684042 0.0066884 0.8592822 WWP1 WW domain containing E3 ubiquitin protein ligase 1 1658 +/- 161 2181 +/- 165 1410 +/- 176 1562 +/- 24 -0.003309 0.0466357 0.43057 0.0263697 0.3583863 XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-bre 1545 +/- 90 1864 +/- 59 1424 +/- 33 1774 +/- 97 -0.034978 0.0164915 0.019361 0.2486474 0.11865 YIF1A Yip1 interacting factor homolog A (S. cerevisiae) 1268 +/- 101 1457 +/- 104 804 +/- 64 1043 +/- 52 -0.000185 0.2207861 0.017631 0.1303022 0.2988299 YIPF1 Yip1 domain family, member 1 1334 +/- 99 1431 +/- 121 950 +/- 44 1022 +/- 35 -0.000469 0.5488273 0.238303 0.4843735 0.4709888 YIPF6 Yip1 domain family, member 6 3308 +/- 117 3207 +/- 46 3070 +/- 67 2974 +/- 54 -0.006007 -0.451895 -0.290677 0.649032 0.1455605 YPEL2 Yippee-like 2 (Drosophila) 365 +/- 62 481 +/- 70 257 +/- 19 243 +/- 15 -0.005996 0.2427428 -0.588208 0.0808224 0.373383 ZA20D1 Zinc finger, A20 domain containing 1 797 +/- 84 1133 +/- 185 517 +/- 38 609 +/- 38 -0.00383 0.1419004 0.125868 0.0364418 0.0617364 ZA20D2 zinc finger, A20 domain containing 2 11645 +/- 435 10719 +/- 480 10212 +/- 434 9777 +/- 415 -0.012691 -0.183482 -0.48745 0.127798 0.7192585

S2-genes differentially expressed across region.xls Page 75 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ZBTB3 zinc finger and BTB domain containing 3 584 +/- 48 622 +/- 34 503 +/- 31 546 +/- 14 -0.029169 0.5292432 0.246727 0.1762176 0.2991777 ZBTB5 zinc finger and BTB domain containing 5 695 +/- 27 675 +/- 14 639 +/- 23 595 +/- 12 -0.023594 -0.524185 -0.126431 0.56804 0.3206235 ZC3H13 Zinc finger CCCH-type containing 13 2082 +/- 90 1605 +/- 162 1861 +/- 75 1362 +/- 193 -0.012616 -0.0334 -0.05896 0.0151073 0.0145363 ZC3H5 zinc finger CCCH-type containing 5 511 +/- 50 651 +/- 39 464 +/- 35 503 +/- 41 -0.035672 0.0536802 0.490227 0.1864741 0.5588391 ZDHHC14 zinc finger, DHHC-type containing 14 2530 +/- 306 2466 +/- 164 1723 +/- 79 1804 +/- 73 -0.003016 -0.858581 0.468934 0.9226812 0.293288 ZDHHC20 zinc finger, DHHC-type containing 20 838 +/- 63 1262 +/- 183 760 +/- 30 734 +/- 39 -0.035345 0.0697697 -0.604544 0.0126002 0.9743389 ZDHHC4 zinc finger, DHHC-type containing 4 469 +/- 18 399 +/- 36 377 +/- 25 354 +/- 10 -0.009186 -0.127043 -0.409293 0.2066598 0.5654692 ZDHHC6 zinc finger, DHHC-type containing 6 2052 +/- 177 1740 +/- 44 1711 +/- 38 1629 +/- 53 -0.03551 -0.140014 -0.247338 0.1097199 0.1083696 ZDHHC9 zinc finger, DHHC-type containing 9 653 +/- 64 929 +/- 196 443 +/- 58 569 +/- 38 -0.020696 0.2288252 0.106911 0.0322781 0.1316479 ZFAND1 zinc finger, AN1-type domain 1 806 +/- 46 867 +/- 25 708 +/- 51 802 +/- 26 -0.044217 0.2822837 0.144424 0.7932191 0.5636639 ZFAND2A zinc finger, AN1-type domain 2A 1098 +/- 80 990 +/- 53 911 +/- 72 884 +/- 28 -0.001556 -0.291699 -0.733123 0.6018191 0.955744 ZFAND2B zinc finger, AN1-type domain 2B 916 +/- 53 963 +/- 40 801 +/- 19 841 +/- 33 -0.013467 0.4962933 0.331667 0.5859159 0.0196032 ZFAND3 zinc finger, AN1-type domain 3 2097 +/- 52 2250 +/- 65 1871 +/- 52 1949 +/- 73 -0.00022 0.0989892 0.410971 0.2255063 0.8483913 ZFHX1B Zinc finger homeobox 1b 1252 +/- 143 1470 +/- 218 1060 +/- 90 1093 +/- 95 -0.009753 0.426942 0.807767 0.177423 0.5339198 ZFYVE16 zinc finger, FYVE domain containing 16 246 +/- 40 362 +/- 35 189 +/- 22 192 +/- 20 -0.005063 0.0558115 0.915161 0.0155606 0.1767158 ZFYVE21 zinc finger, FYVE domain containing 21 3246 +/- 159 3737 +/- 247 2783 +/- 44 3064 +/- 118 -0.0075 0.1303818 0.074415 0.0568761 0.0938414 ZKSCAN1 zinc finger with KRAB and SCAN domains 1 1152 +/- 95 1766 +/- 191 984 +/- 18 1287 +/- 34 -0.037284 0.0226658 0.000206 0.0075827 0.0004425 ZNF134 zinc finger protein 134 (clone pHZ-15) 473 +/- 12 593 +/- 38 437 +/- 27 544 +/- 37 -0.049071 0.0233991 0.04835 0.0086541 0.3033516 ZNF148 zinc finger protein 148 (pHZ-52) 3030 +/- 203 3016 +/- 105 2838 +/- 165 2665 +/- 78 -0.047642 -0.952843 -0.375087 0.750964 0.5023352 ZNF161 zinc finger protein 161 1955 +/- 205 2188 +/- 214 1741 +/- 43 1557 +/- 39 -0.029816 0.4513335 -0.01118 0.120171 0.0720792 ZNF211 zinc finger protein 211 881 +/- 58 788 +/- 34 705 +/- 45 678 +/- 28 -0.001422 -0.202526 -0.619466 0.303169 0.9279066 ZNF217 zinc finger protein 217 147 +/- 16 150 +/- 10 132 +/- 17 106 +/- 14 -0.018115 0.8693352 -0.283582 0.7367072 0.4735861 ZNF22 zinc finger protein 22 (KOX 15) 1474 +/- 69 1454 +/- 59 1350 +/- 37 1277 +/- 19 -0.007635 -0.826142 -0.122989 0.6502111 0.1876815 ZNF281 Zinc finger protein 281 3309 +/- 170 2979 +/- 275 2498 +/- 75 2219 +/- 101 -0.000654 -0.336235 -0.058241 0.9813794 0.382401 ZNF289 zinc finger protein 289, ID1 regulated 2314 +/- 152 2528 +/- 129 1889 +/- 71 1863 +/- 86 -0.001893 0.3076602 -0.818247 0.2238748 0.5133445 ZNF300 zinc finger protein 300 835 +/- 75 879 +/- 60 595 +/- 19 581 +/- 5 -0.000192 0.6565088 -0.510905 0.5564632 0.2056708 ZNF313 zinc finger protein 313 694 +/- 43 845 +/- 53 607 +/- 21 631 +/- 16 -0.006952 0.0520928 0.396094 0.033531 0.7041382 ZNF336 zinc finger protein 336 1688 +/- 83 1861 +/- 80 1511 +/- 33 1629 +/- 15 -0.001594 0.1653536 0.013896 0.0909008 0.1093542 ZNF337 zinc finger protein 337 717 +/- 131 465 +/- 26 573 +/- 81 445 +/- 36 -0.042234 -0.113773 -0.193152 0.3612534 0.6307567 ZNF407 zinc finger protein 407 796 +/- 53 855 +/- 36 646 +/- 25 714 +/- 22 -0.004197 0.3899288 0.070591 0.6391975 0.4100433 ZNF426 zinc finger protein 426 477 +/- 14 603 +/- 52 419 +/- 16 393 +/- 44 -0.001146 0.0609532 -0.606598 0.1545061 0.1516937 ZNF443 zinc finger protein 443 305 +/- 12 327 +/- 14 278 +/- 12 250 +/- 10 -0.005324 0.2674538 -0.100752 0.2907618 0.0591832 ZNF469 zinc finger protein 469 394 +/- 35 350 +/- 17 320 +/- 12 250 +/- 18 -0.002244 -0.294886 -0.015176 0.2768855 0.1902113 ZNF488 zinc finger protein 488 981 +/- 105 851 +/- 94 602 +/- 64 629 +/- 23 -0.000462 -0.378014 0.69765 0.6099653 0.5675187 ZNF533 zinc finger protein 533 789 +/- 59 829 +/- 51 608 +/- 84 643 +/- 77 -0.008702 0.6176858 0.765257 0.5260967 0.9135945 ZNF536 zinc finger protein 536 1336 +/- 254 1954 +/- 342 1012 +/- 67 1119 +/- 56 -0.030434 0.1800763 0.250432 0.044143 0.1020454 ZNF545 zinc finger protein 545 438 +/- 16 501 +/- 24 393 +/- 16 427 +/- 31 -0.002633 0.0550814 0.36234 0.0035827 0.1350272 ZNF561 zinc finger protein 561 444 +/- 41 512 +/- 38 329 +/- 24 397 +/- 16 -0.001359 0.2492244 0.046431 0.617956 0.0441045 ZNF565 zinc finger protein 565 993 +/- 165 973 +/- 84 880 +/- 111 771 +/- 40 -0.041348 -0.916358 -0.386275 0.5250892 0.8649062 ZNF571 zinc finger protein 571 216 +/- 9 212 +/- 8 197 +/- 16 180 +/- 13 -0.020566 -0.769492 -0.441984 0.9857831 0.2785937 ZNF576 zinc finger protein 576 565 +/- 15 524 +/- 23 520 +/- 16 472 +/- 34 -0.015052 -0.164166 -0.247143 0.0162655 0.1097252 ZNF622 zinc finger protein 622 946 +/- 28 1045 +/- 22 741 +/- 18 848 +/- 18 -1.86E-05 0.0219261 0.002302 0.0210693 0.0499573 ZNF641 zinc finger protein 641 882 +/- 68 828 +/- 35 690 +/- 34 686 +/- 43 -0.003444 -0.505211 -0.950144 0.8445956 0.3360656 ZNF671 zinc finger protein 671 730 +/- 18 711 +/- 28 695 +/- 19 663 +/- 27 -0.047893 -0.569133 -0.363161 0.1577803 0.0269636 ZNF692 zinc finger protein 692 586 +/- 28 567 +/- 43 502 +/- 20 514 +/- 36 -0.000916 -0.711215 0.781328 0.7696669 0.3736862

S2-genes differentially expressed across region.xls Page 76 Symbol Description Young CA Aged CA Young DG Aged DG CA v DG CA Y v A DG Y v A CA to PMI DG to PMI ZNF697 zinc finger protein 697 458 +/- 22 383 +/- 9 394 +/- 27 303 +/- 1 -0.000306 -0.018619 -0.020789 0.0263098 0.033517 ZNF703 zinc finger protein 703 395 +/- 42 381 +/- 50 311 +/- 25 268 +/- 13 -0.021177 -0.837298 -0.16919 0.6603022 0.2147872 ZNF710 zinc finger protein 710 610 +/- 19 561 +/- 21 545 +/- 17 515 +/- 13 -0.012112 -0.118339 -0.205276 0.0168475 0.7738473 ZNF9 zinc finger protein 9 6431 +/- 219 5316 +/- 196 5728 +/- 139 4867 +/- 76 -0.000164 -0.00362 -0.000742 0.0312627 0.0114342 ZNFX1 zinc finger, NFX1-type containing 1 1163 +/- 84 1153 +/- 33 968 +/- 25 1002 +/- 18 -0.004144 -0.916447 0.310451 0.8141582 0.4752647 ZNHIT1 zinc finger, HIT type 1 2326 +/- 104 2521 +/- 87 1892 +/- 10 1858 +/- 27 -0.000186 0.1816358 -0.289607 0.3176995 0.2491168 ZSWIM6 zinc finger, SWIM-type containing 6 3973 +/- 250 3673 +/- 404 2866 +/- 104 2695 +/- 171 -0.00067 -0.544998 -0.422074 0.7667326 0.7222284

S2-genes differentially expressed across region.xls Page 77 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Up in Both Rat and Rhesus Aging Acacb acetyl-Coenzyme A carboxylase beta 124 +/- 17 175 +/- 13 138 +/- 12 164 +/- 12 0.9431665 0.0376355 0.1522192 Adam10 ADAM metallopeptidase domain 10 1557 +/- 99 1808 +/- 84 1420 +/- 70 1612 +/- 46 -0.0535771 0.0837127 0.0491171 Akr7a2 aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) 1175 +/- 77 1379 +/- 69 853 +/- 46 1153 +/- 50 -0.0004630 0.0781494 0.0018406 Akr7a3 aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) 722 +/- 43 917 +/- 15 718 +/- 9 959 +/- 30 0.6341318 0.0049548 0.0007634 Anp32b acidic (leucine-rich) nuclear phosphoprotein 32 family, member B 5261 +/- 630 6605 +/- 629 4342 +/- 135 4863 +/- 137 -0.0208286 0.1620245 0.0241818 Apod apolipoprotein D 1694 +/- 156 2698 +/- 413 1143 +/- 60 1879 +/- 176 -0.0020709 0.0605189 0.0110462 Arpc1b actin related protein 2/3 complex, subunit 1B, 41kDa 265 +/- 16 338 +/- 19 206 +/- 20 235 +/- 20 -0.0011827 0.0141484 0.3219935 B2m beta-2-microglobulin 7946 +/- 519 10622 +/- 678 6616 +/- 572 9837 +/- 646 -0.0207958 0.0114966 0.0051473 Bzrp benzodiazapine receptor (peripheral) 565 +/- 74 798 +/- 62 374 +/- 21 504 +/- 36 -0.0033553 0.0377401 0.0184873 Capn2 calpain 2, (m/II) large subunit 2440 +/- 204 3016 +/- 132 1931 +/- 137 2492 +/- 70 -0.0002235 0.0430506 0.0075702 Cast calpastatin 990 +/- 127 1424 +/- 112 822 +/- 41 1048 +/- 19 -0.0116342 0.0287645 0.0015796 Cd44 CD44 molecule (Indian blood group) 390 +/- 49 579 +/- 97 642 +/- 74 919 +/- 44 0.0005290 0.1243777 0.0129636 Cd74 CD74 molecule, major histocompatibility complex, class II invariant chain 1810 +/- 283 3714 +/- 547 1215 +/- 171 2317 +/- 340 -0.0160328 0.0161530 0.0276289 Cd81 CD81 molecule 13811 +/- 551 15425 +/- 255 12732 +/- 477 14927 +/- 743 -0.0999738 0.0323751 0.0418025 Ctsl cathepsin L 1295 +/- 116 1543 +/- 70 1117 +/- 86 1413 +/- 26 -0.1059453 0.1024043 0.0172698 Ctss cathepsin S 157 +/- 19 238 +/- 20 157 +/- 21 194 +/- 33 -0.2573411 0.0137798 0.3711978 Dnm2 dynamin 2 623 +/- 66 744 +/- 63 541 +/- 14 626 +/- 29 -0.0401533 0.2143312 0.0406403 Edg2 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 5091 +/- 735 6481 +/- 945 2992 +/- 289 3972 +/- 198 -0.0065174 0.2741158 0.0220017 Eef1a1 eukaryotic translation elongation factor 1 alpha 1 11705 +/- 649 13512 +/- 606 11114 +/- 370 12852 +/- 588 -0.1952757 0.0694277 0.0412874 Eltd1 EGF, latrophilin and seven transmembrane domain containing 1 216 +/- 15 319 +/- 20 190 +/- 19 228 +/- 17 -0.0267015 0.0022995 0.1652497 Emp3 epithelial membrane protein 3 385 +/- 23 473 +/- 25 311 +/- 21 374 +/- 24 -0.0017385 0.0295194 0.0811556 Faim Fas apoptotic inhibitory molecule 608 +/- 41 920 +/- 63 711 +/- 51 974 +/- 37 0.1126147 0.0027375 0.0024603 Gabarap GABA(A) receptor-associated protein 11702 +/- 341 11960 +/- 190 10752 +/- 157 11400 +/- 130 -0.0017698 0.5282196 0.0112989 Grik1 glutamate receptor, ionotropic, kainate 1 107 +/- 11 355 +/- 42 65 +/- 9 194 +/- 15 -0.0057951 0.0015561 0.0001762 Gsn gelsolin (amyloidosis, Finnish type) 4180 +/- 541 5269 +/- 560 2845 +/- 206 3577 +/- 214 -0.0011239 0.1927344 0.0363701 Hmgn2 high-mobility group nucleosomal binding domain 2 197 +/- 23 265 +/- 10 221 +/- 5 255 +/- 33 0.5194376 0.0313723 0.3765625 Hrasls3 HRAS-like suppressor 3 3691 +/- 429 4571 +/- 653 2117 +/- 93 2770 +/- 179 -0.0016972 0.2900760 0.0173676 Igf1r Insulin-like growth factor 1 receptor 545 +/- 60 811 +/- 50 668 +/- 61 725 +/- 86 0.4020595 0.0071628 0.6081189 Insig1 insulin induced gene 1 1796 +/- 160 2510 +/- 276 2251 +/- 175 2909 +/- 224 0.1445151 0.0554641 0.0492649 Lgmn legumain 2017 +/- 149 2333 +/- 156 1847 +/- 52 2339 +/- 106 -0.1475833 0.1751615 0.0060751 Lyz lysozyme (renal amyloidosis) 354 +/- 21 541 +/- 71 247 +/- 38 330 +/- 29 -0.0022358 0.0455740 0.1170922 Mobp myelin-associated oligodendrocyte basic protein 3329 +/- 750 6661 +/- 1634 1660 +/- 465 3347 +/- 458 -0.0295547 0.1061680 0.0297469 Mpeg1 macrophage expressed gene 1 612 +/- 60 826 +/- 70 399 +/- 27 583 +/- 25 -0.0014237 0.0432552 0.0007380 Nap1l1 nucleosome assembly protein 1-like 1 1463 +/- 33 1829 +/- 95 1400 +/- 79 1475 +/- 74 -0.0382093 0.0102000 0.4998684 Ninj2 ninjurin 2 672 +/- 49 990 +/- 158 361 +/- 35 494 +/- 25 -0.0022306 0.1042603 0.0143672 Pabpc1 Poly(A) binding protein, cytoplasmic 1 183 +/- 21 254 +/- 18 240 +/- 24 272 +/- 26 0.0738248 0.0283996 0.4009303 Pex11a Peroxisomal biogenesis factor 11A 115 +/- 9 142 +/- 7 134 +/- 13 144 +/- 10 0.2226551 0.0408075 0.5452803 Pkn1 protein kinase N1 1025 +/- 87 1327 +/- 101 952 +/- 42 1034 +/- 60 -0.0515847 0.0479771 0.2991313 Plekhb1 pleckstrin homology domain containing, family B (evectins) member 1 6842 +/- 885 9643 +/- 1345 4705 +/- 239 6453 +/- 287 -0.0104695 0.1174381 0.0014521 Ppap2b phosphatidic acid phosphatase type 2B 2720 +/- 167 3718 +/- 213 2634 +/- 127 2728 +/- 239 -0.0402748 0.0045712 0.7413099 Prkch protein kinase C, eta 181 +/- 18 255 +/- 18 129 +/- 12 131 +/- 19 -0.0007280 0.0168632 0.9434525 Psmb9 proteasome (prosome, macropain) subunit, beta type, 9 262 +/- 10 349 +/- 12 233 +/- 6 295 +/- 8 -0.0013274 0.0002752 0.0003673 Psme2 proteasome (prosome, macropain) activator subunit 2 (PA28 beta) 821 +/- 80 977 +/- 77 784 +/- 43 921 +/- 39 -0.6965119 0.1883069 0.0424353 Rab7 RAB7, member RAS oncogene family 9341 +/- 493 9851 +/- 264 7242 +/- 277 8105 +/- 249 -0.0000127 0.3893238 0.0456579 Rnase4 ribonuclease, RNase A family, 4 204 +/- 17 250 +/- 22 133 +/- 12 240 +/- 28 -0.0035331 0.1324862 0.0157391 S100a6 S100 calcium binding protein A6 (calcyclin) 923 +/- 64 1156 +/- 72 682 +/- 81 1085 +/- 58 -0.0020704 0.0359624 0.0031494 Sds serine dehydratase 467 +/- 22 597 +/- 50 542 +/- 35 590 +/- 52 0.1669323 0.0486479 0.4692068 Serpina1 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), mem 442 +/- 80 836 +/- 138 331 +/- 29 562 +/- 91 -0.0572560 0.0392633 0.0620666 Sgk serum/glucocorticoid regulated kinase 1969 +/- 150 2626 +/- 225 1220 +/- 179 1632 +/- 102 -0.0005393 0.0390527 0.0816104 Slc15a3 solute carrier family 15, member 3 255 +/- 27 313 +/- 19 234 +/- 7 271 +/- 8 -0.0203261 0.1124988 0.0058542 Slc2a5 solute carrier family 2 (facilitated glucose/fructose transporter), member 5 534 +/- 45 707 +/- 82 446 +/- 26 613 +/- 49 -0.1667049 0.1022042 0.0238476 Snx1 Sorting nexin 1 172 +/- 19 261 +/- 27 175 +/- 13 234 +/- 16 -0.7803549 0.0242684 0.0202648 Ssr3 signal sequence receptor, gamma (translocon-associated protein gamma) 1416 +/- 40 1776 +/- 112 1570 +/- 46 1920 +/- 158 0.0821917 0.0221610 0.0903644 Tap1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) 270 +/- 14 334 +/- 23 295 +/- 13 305 +/- 17 -0.8615634 0.0462617 0.6676726 Tf transferrin 7019 +/- 1463 9974 +/- 1622 4189 +/- 354 6128 +/- 572 -0.0124450 0.2061852 0.0241712 Tjp2 tight junction protein 2 (zona occludens 2) 2291 +/- 204 3125 +/- 237 2144 +/- 139 2859 +/- 124 -0.1469352 0.0240802 0.0040534 Tnxa tenascin XA pseudogene 497 +/- 80 915 +/- 160 295 +/- 39 476 +/- 26 -0.0127490 0.0506152 0.0042067 Tyrobp TYRO protein tyrosine kinase binding protein 1055 +/- 57 1424 +/- 146 717 +/- 24 899 +/- 63 -0.0032704 0.0533008 0.0413257 Waspip Wiskott-Aldrich syndrome protein interacting protein 453 +/- 53 836 +/- 159 339 +/- 48 483 +/- 31 -0.0313687 0.0615464 0.0366266

S3- Aging related genes in rhesus and rat.xls Page 1 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Xrcc5 X-ray repair complementing defective repair in Chinese hamster cells 5 1545 +/- 90 1864 +/- 59 1424 +/- 33 1774 +/- 97 -0.0349779 0.0164915 0.0193613 Down in Both Rat and Rhesus Aging Studies Abtb2 Ankyrin repeat and BTB (POZ) domain containing 2 654 +/- 122 485 +/- 39 505 +/- 32 342 +/- 18 -0.0301841 -0.2365310 -0.0022652 Acat1 acetyl-Coenzyme A acetyltransferase 1 (acetoacetyl Coenzyme A thiolase) 4099 +/- 188 3515 +/- 106 3250 +/- 137 3238 +/- 137 -0.0074670 -0.0270457 -0.9542733 Accn1 amiloride-sensitive cation channel 1, neuronal (degenerin) 1925 +/- 136 1421 +/- 164 1179 +/- 59 900 +/- 42 -0.0002544 -0.0406562 -0.0043916 Add1 adducin 1 (alpha) 4262 +/- 135 3716 +/- 140 3921 +/- 116 3527 +/- 163 -0.0002315 -0.0186129 -0.0860880 Ap2b1 adaptor-related protein complex 2, beta 1 subunit 7691 +/- 354 5572 +/- 446 5527 +/- 121 4060 +/- 189 -0.0001268 -0.0042974 -0.0003231 Atp2c1 ATPase, Ca++ transporting, type 2C, member 1 2029 +/- 118 1514 +/- 111 1497 +/- 112 1332 +/- 42 -0.0034015 -0.0098068 -0.2133815 Bcl10 B-cell CLL/lymphoma 10 367 +/- 32 332 +/- 22 459 +/- 20 380 +/- 15 0.0102893 -0.3959548 -0.0114145 Capns1 calpain, small subunit 1 7708 +/- 486 6177 +/- 323 7211 +/- 399 6252 +/- 282 -0.3532394 -0.0283825 -0.0828269 Capzb capping protein (actin filament) muscle Z-line, beta 8247 +/- 582 6857 +/- 252 6783 +/- 248 5959 +/- 245 -0.0061274 -0.0655422 -0.0425001 Chka choline kinase alpha 607 +/- 41 586 +/- 44 764 +/- 36 646 +/- 32 0.0304713 -0.7389491 -0.0352925 Fxyd7 FXYD domain containing ion transport regulator 7 3435 +/- 178 2940 +/- 109 4078 +/- 119 3624 +/- 147 0.0008447 -0.0440768 -0.0424292 Fyn FYN oncogene related to SRC, FGR, YES 243 +/- 42 94 +/- 25 298 +/- 27 175 +/- 27 0.0193077 -0.0148174 -0.0109022 Ggh gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) 1157 +/- 71 923 +/- 51 1159 +/- 47 1032 +/- 52 0.2146496 -0.0247358 -0.1036536 Gnmt glycine N-methyltransferase 171 +/- 11 138 +/- 16 174 +/- 20 111 +/- 11 -0.3479966 -0.1268625 -0.0244554 Gsk3a glycogen synthase kinase 3 alpha 4199 +/- 213 3357 +/- 277 4155 +/- 242 3593 +/- 133 0.7848632 -0.0381389 -0.0784960 H1f0 H1 histone family, member 0 1586 +/- 121 1504 +/- 164 1458 +/- 52 1178 +/- 25 -0.0306539 -0.6962873 -0.0017378 Hagh hydroxyacylglutathione hydrolase 3456 +/- 243 2690 +/- 123 2867 +/- 137 2258 +/- 72 -0.0003267 -0.0244967 -0.0051190 Hnmt histamine N-methyltransferase 396 +/- 32 235 +/- 22 494 +/- 59 338 +/- 17 0.0066641 -0.0025243 -0.0449354 Homer1 homer homolog 1 (Drosophila) 2901 +/- 169 2385 +/- 132 2363 +/- 91 2257 +/- 80 -0.0230777 -0.0381883 -0.4084363 Kif3c kinesin family member 3C 5625 +/- 458 4054 +/- 161 4195 +/- 251 3767 +/- 99 -0.0124291 -0.0169574 -0.1604198 Lphn1 latrophilin 1 3273 +/- 149 2840 +/- 165 3901 +/- 181 3300 +/- 124 0.0011972 -0.0802788 -0.0239780 Map1lc3a microtubule-associated protein 1 light chain 3 alpha 3967 +/- 274 2363 +/- 245 4800 +/- 253 2961 +/- 404 0.0000747 -0.0014419 -0.0063656 Mapk9 Mitogen-activated protein kinase 9 4733 +/- 223 3991 +/- 177 4745 +/- 208 4279 +/- 155 0.3865928 -0.0270888 -0.1070479 Nudt4 nudix (nucleoside diphosphate linked moiety X)-type motif 4 341 +/- 23 281 +/- 33 505 +/- 25 364 +/- 47 0.0001215 -0.1679549 -0.0362152 Pak3 P21 (CDKN1A)-activated kinase 3 227 +/- 18 167 +/- 19 271 +/- 16 203 +/- 14 0.0520762 -0.0482017 -0.0104975 Pkia Protein kinase (cAMP-dependent, catalytic) inhibitor alpha 252 +/- 9 223 +/- 13 277 +/- 14 229 +/- 14 0.1021183 -0.0941440 -0.0411478 Prdx5 peroxiredoxin 5 3941 +/- 95 3412 +/- 66 3326 +/- 71 3314 +/- 37 -0.0089121 -0.0013997 -0.8854836 Ptprn2 protein tyrosine phosphatase, receptor type, N polypeptide 2 6494 +/- 194 5488 +/- 322 6194 +/- 400 5533 +/- 188 -0.5663775 -0.0274318 -0.1780531 Rasgrf1 Ras protein-specific guanine nucleotide-releasing factor 1 125 +/- 16 76 +/- 11 145 +/- 12 104 +/- 10 0.0770149 -0.0314711 -0.0251922 Ryr1 ryanodine receptor 1 (skeletal) 1032 +/- 27 722 +/- 33 993 +/- 101 590 +/- 54 -0.2123464 -0.0000308 -0.0085973 Sema6b sema domain, transmembrane domain (TM), and cytoplasmic domain, 6B 2141 +/- 281 1472 +/- 204 2572 +/- 104 2191 +/- 53 0.0023509 -0.0859556 -0.0130934 Sfxn3 sideroflexin 3 918 +/- 58 841 +/- 34 1028 +/- 50 787 +/- 44 0.5966323 -0.2905131 -0.0054915 Slc31a1 solute carrier family 31 (copper transporters), member 1 325 +/- 19 228 +/- 24 334 +/- 28 277 +/- 30 0.2290539 -0.0117825 -0.1987807 Slco1c1 solute carrier organic anion transporter family, member 1C1 525 +/- 72 446 +/- 45 404 +/- 18 280 +/- 27 -0.0084258 -0.3805519 -0.0063149 Ssb Sjogren syndrome antigen B (autoantigen La) 2114 +/- 118 1777 +/- 99 2359 +/- 70 1882 +/- 138 0.0632167 -0.0546976 -0.0216278 Sstr2 somatostatin receptor 2 181 +/- 26 94 +/- 7 135 +/- 18 66 +/- 7 -0.0294941 -0.0180115 -0.0101966 Ssx2ip synovial sarcoma, X breakpoint 2 interacting protein 256 +/- 17 190 +/- 21 218 +/- 7 166 +/- 12 -0.0858889 -0.0364965 -0.0076264 Stmn2 stathmin-like 2 15575 +/- 785 11635 +/- 678 13350 +/- 985 11673 +/- 910 -0.1115448 -0.0036256 -0.2426939 Thy1 Thy-1 cell surface antigen 9156 +/- 962 5509 +/- 551 8393 +/- 478 6379 +/- 427 0.9248060 -0.0110921 -0.0119199 Ugcg UDP-glucose ceramide glucosyltransferase 1454 +/- 63 1024 +/- 64 1638 +/- 69 1487 +/- 121 0.0160627 -0.0007334 -0.3182540 Xpnpep1 X-prolyl aminopeptidase (aminopeptidase P) 1, soluble 1226 +/- 73 1063 +/- 32 1269 +/- 56 1109 +/- 24 0.3377216 -0.0807381 -0.0356985 Up in Rhesus Aging Studies Only Qtrt1 queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) 256 +/- 22 498 +/- 21 323 +/- 31 587 +/- 36 0.0208109 0.0000148 0.0004756 Rab28 RAB28, member RAS oncogene family 757 +/- 67 1355 +/- 52 860 +/- 38 1404 +/- 71 0.1109075 0.0000472 0.0004438 Igbp1 immunoglobulin (CD79A) binding protein 1 1965 +/- 47 2506 +/- 69 1885 +/- 71 1954 +/- 88 -0.0212182 0.0001316 0.5561280 Sgce sarcoglycan, epsilon 754 +/- 60 1219 +/- 49 868 +/- 51 1204 +/- 28 0.2318045 0.0001575 0.0004831 Rpl13 ribosomal protein L13 3331 +/- 242 4584 +/- 286 2546 +/- 122 3520 +/- 104 -0.0000929 0.0076985 0.0001885 Cct3 chaperonin containing TCP1, subunit 3 (gamma) 2068 +/- 138 2872 +/- 102 1999 +/- 97 2727 +/- 72 -0.0511476 0.0010854 0.0002178 Tfpi tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) 202 +/- 14 337 +/- 20 145 +/- 11 265 +/- 18 -0.0003335 0.0003604 0.0010016 Gpm6B glycoprotein M6B 5149 +/- 133 6879 +/- 254 6195 +/- 386 7340 +/- 323 0.0268381 0.0003929 0.0492732 Rps15A ribosomal protein S15a 14626 +/- 477 16659 +/- 556 11527 +/- 341 13684 +/- 211 -0.0000040 0.0199780 0.0006419 Nasp Nuclear autoantigenic sperm protein (histone-binding) 466 +/- 56 813 +/- 84 458 +/- 42 736 +/- 35 -0.0708711 0.0078378 0.0006624 Rbm3 RNA binding motif (RNP1, RRM) protein 3 669 +/- 111 1358 +/- 95 805 +/- 127 1331 +/- 55 0.3776472 0.0008674 0.0071442 Cox6C cytochrome c oxidase subunit VIc 7080 +/- 300 8976 +/- 497 6419 +/- 237 8142 +/- 260 -0.0271562 0.0110211 0.0009592 Nup62 nucleoporin 62kDa 885 +/- 11 1158 +/- 43 1053 +/- 49 1241 +/- 35 0.0141701 0.0009882 0.0126909 Rps6Kb1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 1507 +/- 66 2010 +/- 89 1589 +/- 57 1912 +/- 83 -0.8305136 0.0013009 0.0138070 Pfkfb2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 531 +/- 38 759 +/- 35 440 +/- 25 533 +/- 18 -0.0007897 0.0013335 0.0146411 Slc11A2 solute carrier family 11 (proton-coupled divalent metal ion transporters), mem 475 +/- 88 1020 +/- 94 461 +/- 69 952 +/- 81 -0.3381127 0.0017448 0.0015062

S3- Aging related genes in rhesus and rat.xls Page 2 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Mybbp1A MYB binding protein (P160) 1a 227 +/- 13 300 +/- 10 267 +/- 21 271 +/- 13 0.7416572 0.0015408 0.8770270 Slc22A18 solute carrier family 22 (organic cation transporter), member 18 269 +/- 23 400 +/- 39 191 +/- 11 317 +/- 21 -0.0021577 0.0198389 0.0016195 Pa2G4 proliferation-associated 2G4, 38kDa 712 +/- 69 939 +/- 45 885 +/- 51 1182 +/- 44 0.0003316 0.0231989 0.0017518 Cbr4 Carbonyl reductase 4 86 +/- 10 138 +/- 8 105 +/- 19 109 +/- 10 -0.8829589 0.0021832 0.8718192 Rab8A RAB8A, member RAS oncogene family 660 +/- 25 807 +/- 25 552 +/- 11 656 +/- 19 -0.0000622 0.0021892 0.0024208 Cd52 CD52 molecule 303 +/- 58 621 +/- 52 251 +/- 51 388 +/- 37 -0.0151501 0.0023325 0.0574541 Psma3 proteasome (prosome, macropain) subunit, alpha type, 3 1537 +/- 38 1871 +/- 67 1481 +/- 61 1913 +/- 144 -0.6547428 0.0025823 0.0363393 Cstb cystatin B (stefin B) 6756 +/- 454 7724 +/- 450 6112 +/- 199 7133 +/- 147 -0.0304870 0.1610221 0.0027462 Icam1 intercellular adhesion molecule 1 (CD54), human rhinovirus receptor 120 +/- 10 185 +/- 13 126 +/- 9 167 +/- 8 -0.5099021 0.0027757 0.0079736 Eif3S7 eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa 2662 +/- 54 2791 +/- 67 2401 +/- 59 2691 +/- 31 -0.0040374 0.1657818 0.0028384 Wbp1 WW domain binding protein 1 1823 +/- 116 2506 +/- 129 1839 +/- 149 2251 +/- 134 -0.1118393 0.0028595 0.0697452 Foxo1A forkhead box O1A (rhabdomyosarcoma) 720 +/- 44 1108 +/- 81 1000 +/- 116 1225 +/- 114 0.0467044 0.0031203 0.1999455 Comt catechol-O-methyltransferase 2761 +/- 143 3402 +/- 147 2879 +/- 124 3569 +/- 121 0.3771375 0.0106563 0.0031852 Hnrpl heterogeneous nuclear ribonucleoprotein L 5232 +/- 292 6250 +/- 170 6161 +/- 225 7259 +/- 144 0.0006335 0.0167305 0.0032344 Kif3A kinesin family member 3A 6022 +/- 351 7526 +/- 490 5756 +/- 353 7952 +/- 415 0.9322618 0.0338848 0.0034460 Gclm glutamate-cysteine ligase, modifier subunit 771 +/- 36 943 +/- 26 851 +/- 27 1048 +/- 44 0.0142308 0.0036647 0.0072509 Crnkl1 Crn, crooked neck-like 1 (Drosophila) 267 +/- 89 512 +/- 56 270 +/- 68 595 +/- 45 0.4404922 0.0464274 0.0036829 Coq3 coenzyme Q3 homolog, methyltransferase (S. cerevisiae) 962 +/- 18 1137 +/- 65 967 +/- 22 1098 +/- 25 -0.8171245 0.0430001 0.0038244 Tas2R14 Taste receptor, type 2, member 14 101 +/- 20 203 +/- 19 104 +/- 21 230 +/- 27 0.3959057 0.0044384 0.0059735 Pigs phosphatidylinositol glycan, class S 2823 +/- 73 3263 +/- 183 2696 +/- 71 3307 +/- 125 -0.8260991 0.0625930 0.0044961 Golga2 golgi autoantigen, golgin subfamily a, 2 80 +/- 6 112 +/- 6 118 +/- 19 140 +/- 9 0.0095632 0.0047140 0.3287570 Pdk3 Pyruvate dehydrogenase kinase, isozyme 3 704 +/- 98 960 +/- 111 993 +/- 129 1593 +/- 94 0.0004963 0.1138820 0.0047699 Exoc7 exocyst complex component 7 228 +/- 32 391 +/- 33 285 +/- 12 351 +/- 36 0.5131319 0.0049690 0.1443382 Slc12A9 solute carrier family 12 (potassium/chloride transporters), member 9 1093 +/- 101 1413 +/- 80 1023 +/- 47 1257 +/- 43 -0.0694043 0.0331331 0.0050508 Hla-Dma major histocompatibility complex, class II, DM alpha 100 +/- 12 213 +/- 26 81 +/- 17 132 +/- 17 -0.0381701 0.0051739 0.0622950 Ptk2 PTK2 protein tyrosine kinase 2 352 +/- 24 487 +/- 29 419 +/- 31 528 +/- 44 0.0914861 0.0053450 0.0810219 Pvalb parvalbumin 391 +/- 24 469 +/- 50 287 +/- 22 418 +/- 28 -0.0202594 0.1995472 0.0058419 Pgam1 phosphoglycerate mutase 1 (brain) 11785 +/- 417 13568 +/- 474 10224 +/- 320 12402 +/- 471 -0.0014795 0.0183042 0.0060177 Glg1 golgi apparatus protein 1 4000 +/- 666 6938 +/- 706 3906 +/- 536 6715 +/- 578 -0.6644467 0.0127739 0.0064554 Ctnnb1 Catenin (cadherin-associated protein), beta 1, 88kDa 80 +/- 20 240 +/- 38 98 +/- 21 283 +/- 59 0.2020876 0.0064958 0.0312557 Etfdh electron-transferring-flavoprotein dehydrogenase 382 +/- 17 463 +/- 16 356 +/- 16 395 +/- 22 -0.0118249 0.0066056 0.1909748 Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit 294 +/- 22 397 +/- 21 287 +/- 16 334 +/- 20 -0.0730364 0.0066895 0.1038060 Prkab2 protein kinase, AMP-activated, beta 2 non-catalytic subunit 697 +/- 64 885 +/- 68 772 +/- 23 952 +/- 40 0.0795326 0.0722526 0.0067765 Gstt1 Glutathione S-transferase theta 1 180 +/- 17 259 +/- 19 171 +/- 15 240 +/- 13 -0.6973574 0.0126606 0.0074401 Aldh3A1 aldehyde dehydrogenase 3 family, memberA1 99 +/- 11 267 +/- 57 115 +/- 22 357 +/- 54 0.1926285 0.0317146 0.0081362 Rtcd1 RNA terminal phosphate cyclase domain 1 145 +/- 9 194 +/- 12 137 +/- 5 174 +/- 10 -0.1042368 0.0082334 0.0170400 Hdac6 histone deacetylase 6 858 +/- 46 1082 +/- 96 763 +/- 14 907 +/- 33 -0.0110853 0.0726796 0.0086251 Ppia peptidylprolyl isomerase A (cyclophilin A) 281 +/- 45 463 +/- 32 349 +/- 57 440 +/- 15 0.1857574 0.0089627 0.1748525 Fbp1 fructose-1,6-bisphosphatase 1 89 +/- 10 125 +/- 32 81 +/- 8 136 +/- 13 0.7590811 0.3232018 0.0091964 Scfd1 sec1 family domain containing 1 83 +/- 2 121 +/- 10 105 +/- 6 109 +/- 8 0.7154337 0.0094376 0.7336046 Acaa2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A 589 +/- 56 854 +/- 62 529 +/- 31 645 +/- 33 -0.0389698 0.0097889 0.0301529 Rps24 ribosomal protein S24 19440 +/- 754 21550 +/- 931 17416 +/- 712 21538 +/- 983 -0.0889793 0.1101938 0.0101360 Nudt5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 884 +/- 75 1105 +/- 67 672 +/- 11 954 +/- 63 -0.0035642 0.0533906 0.0101613 Cycs cytochrome c, somatic 1114 +/- 69 1523 +/- 105 1124 +/- 93 1485 +/- 52 -0.9949297 0.0103416 0.0101909 Nrp2 neuropilin 2 178 +/- 27 256 +/- 23 279 +/- 17 347 +/- 12 0.0018426 0.0546684 0.0102310 Gja1 gap junction protein, alpha 1, 43kDa (connexin 43) 11658 +/- 328 13350 +/- 420 12800 +/- 751 13085 +/- 225 0.3273443 0.0106693 0.7288143 Sfxn5 Sideroflexin 5 157 +/- 8 208 +/- 14 190 +/- 13 226 +/- 9 0.0109338 0.0109709 0.0519195 Emp2 epithelial membrane protein 2 131 +/- 20 209 +/- 17 138 +/- 17 214 +/- 16 0.4980607 0.0133384 0.0112681 Smyd2 SET and MYND domain containing 2 109 +/- 17 181 +/- 16 144 +/- 19 192 +/- 26 0.0151438 0.0113553 0.1804340 Gnb2L1 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 8227 +/- 314 9584 +/- 311 7947 +/- 439 9269 +/- 228 -0.1775069 0.0118215 0.0303457 Tgoln2 trans-golgi network protein 2 6539 +/- 384 7975 +/- 222 6466 +/- 326 7084 +/- 273 -0.1784386 0.0119243 0.1799392 Dpm2 dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit 870 +/- 18 1041 +/- 46 787 +/- 41 847 +/- 24 -0.0045859 0.0119429 0.2420499 Psmd4 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 2476 +/- 113 2897 +/- 73 2016 +/- 75 2132 +/- 95 -0.0000149 0.0127068 0.3640977 Nr3C1 Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) 263 +/- 52 681 +/- 115 326 +/- 52 464 +/- 66 -0.2665340 0.0127770 0.1384024 Mapre1 microtubule-associated protein, RP/EB family, member 1 1826 +/- 135 2236 +/- 182 1327 +/- 73 1595 +/- 31 -0.0003278 0.1033306 0.0128438 Kif5A kinesin family member 5A 2488 +/- 627 4967 +/- 527 2216 +/- 519 3839 +/- 250 -0.0254831 0.0131554 0.0255153 Ptpn21 Protein tyrosine phosphatase, non-receptor type 21 156 +/- 37 305 +/- 48 153 +/- 15 276 +/- 31 -0.1743656 0.0360460 0.0133489 Cugbp2 CUG triplet repeat, RNA binding protein 2 1263 +/- 229 2289 +/- 319 1772 +/- 270 3161 +/- 347 0.0089862 0.0280230 0.0134923 Itgb4 integrin, beta 4 433 +/- 101 626 +/- 98 854 +/- 90 1205 +/- 70 0.0000403 0.1993225 0.0136368 Ncam1 neural cell adhesion molecule 1 374 +/- 80 1218 +/- 237 262 +/- 43 746 +/- 266 -0.0316066 0.0144410 0.1428245

S3- Aging related genes in rhesus and rat.xls Page 3 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Thedc1 thioesterase domain containing 1 35 +/- 9 287 +/- 70 65 +/- 11 160 +/- 25 -0.2943253 0.0152784 0.0148744 Cntn1 contactin 1 1106 +/- 69 1435 +/- 87 1088 +/- 58 1302 +/- 69 -0.2678585 0.0149104 0.0435794 Hsd11B1 hydroxysteroid (11-beta) dehydrogenase 1 182 +/- 39 214 +/- 25 78 +/- 15 136 +/- 13 -0.0095581 0.5032791 0.0150164 Rchy1 ring finger and CHY zinc finger domain containing 1 986 +/- 103 1349 +/- 54 982 +/- 90 1245 +/- 17 -0.3680508 0.0152129 0.0317875 Slc4A7 solute carrier family 4, sodium bicarbonate cotransporter, member 7 1387 +/- 232 3599 +/- 647 1815 +/- 290 4114 +/- 614 0.0968112 0.0171541 0.0156976 Bax BCL2-associated X protein 831 +/- 23 877 +/- 24 728 +/- 13 807 +/- 21 -0.0002679 0.1884430 0.0161400 Ptp4A2 protein tyrosine phosphatase type IVA, member 2 6286 +/- 287 7210 +/- 518 5634 +/- 234 7240 +/- 434 -0.3881091 0.1580916 0.0164181 Atf1 Activating transcription factor 1 660 +/- 47 833 +/- 36 824 +/- 43 894 +/- 69 0.0855870 0.0165201 0.4139316 Itgam integrin, alpha M (complement component 3 receptor 3 subunit) 332 +/- 23 412 +/- 12 323 +/- 26 348 +/- 34 -0.1719967 0.0167668 0.5845841 Ufd1L ubiquitin fusion degradation 1 like (yeast) 1115 +/- 186 1832 +/- 170 1206 +/- 182 1617 +/- 52 -0.8846977 0.0175615 0.0738794 Cxxc4 CXXC finger 4 336 +/- 31 432 +/- 16 266 +/- 21 359 +/- 24 -0.0043239 0.0253939 0.0176697 Mfn2 mitofusin 2 4230 +/- 255 4976 +/- 285 3779 +/- 52 4823 +/- 275 -0.1321544 0.0800305 0.0179208 Surf1 surfeit 1 1906 +/- 52 2026 +/- 101 1667 +/- 86 1959 +/- 45 -0.0013522 0.3240518 0.0181346 Mcam melanoma cell adhesion molecule 1170 +/- 212 1662 +/- 293 589 +/- 48 817 +/- 61 -0.0043207 0.2075345 0.0187982 Cdk5Rap3 CDK5 regulatory subunit associated protein 3 2040 +/- 139 2326 +/- 68 1983 +/- 100 2344 +/- 76 -0.9127573 0.1059012 0.0188578 Prss21 protease, serine, 21 (testisin) 154 +/- 18 182 +/- 13 167 +/- 4 246 +/- 21 0.0202638 0.2276938 0.0191110 Chst3 carbohydrate (chondroitin 6) sulfotransferase 3 639 +/- 84 773 +/- 125 385 +/- 5 482 +/- 26 -0.0051464 0.3983593 0.0194651 Usp14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) 1553 +/- 103 1788 +/- 135 1582 +/- 72 1981 +/- 114 0.4919039 0.1987562 0.0210156 Mbp myelin basic protein 267 +/- 30 382 +/- 30 198 +/- 26 233 +/- 24 -0.0020672 0.0221518 0.3568898 Mrps15 mitochondrial ribosomal protein S15 1027 +/- 36 1142 +/- 68 917 +/- 42 1056 +/- 29 -0.0564321 0.1731679 0.0239796 Pgam2 phosphoglycerate mutase 2 (muscle) 175 +/- 19 219 +/- 19 163 +/- 19 222 +/- 6 -0.9459678 0.1251703 0.0241524 Tfpt TCF3 (E2A) fusion partner (in childhood Leukemia) 369 +/- 36 499 +/- 34 283 +/- 25 365 +/- 25 -0.0009157 0.0243374 0.0424801 Cald1 caldesmon 1 84 +/- 6 130 +/- 15 132 +/- 8 136 +/- 6 0.0056260 0.0251705 0.7102261 Lifr leukemia inhibitory factor receptor alpha 2392 +/- 272 3256 +/- 164 2200 +/- 139 2374 +/- 110 -0.0238676 0.0256460 0.3505753 Megf10 multiple EGF-like-domains 10 775 +/- 88 1122 +/- 99 648 +/- 44 768 +/- 24 -0.0257209 0.0259818 0.0456957 Znf384 zinc finger protein 384 657 +/- 34 714 +/- 19 682 +/- 19 758 +/- 21 0.1644873 0.1755146 0.0262426 Emd emerin (Emery-Dreifuss muscular dystrophy) 883 +/- 36 950 +/- 27 898 +/- 19 978 +/- 23 0.5449752 0.1708619 0.0266424 Cul5 cullin 5 2810 +/- 145 3330 +/- 140 3029 +/- 94 3332 +/- 99 0.3701530 0.0273875 0.0549282 Timm17A translocase of inner mitochondrial membrane 17 homolog A (yeast) 4599 +/- 193 5229 +/- 399 4545 +/- 37 5734 +/- 354 0.2577344 0.1964322 0.0279685 Plxna3 plexin A3 205 +/- 22 318 +/- 36 319 +/- 36 416 +/- 24 0.0173357 0.0281193 0.0548365 Tjp1 tight junction protein 1 (zona occludens 1) 881 +/- 94 1108 +/- 148 851 +/- 67 1056 +/- 37 -0.3393285 0.2295937 0.0297285 Snap91 synaptosomal-associated protein, 91kDa homolog (mouse) 10224 +/- 579 10352 +/- 809 10870 +/- 483 12419 +/- 359 0.0166552 0.9003596 0.0307305 Cox5B cytochrome c oxidase subunit Vb 6825 +/- 788 9221 +/- 528 4736 +/- 885 7292 +/- 122 -0.0000318 0.0331089 0.0338796 Ada adenosine deaminase 265 +/- 19 352 +/- 29 341 +/- 51 373 +/- 25 0.1306048 0.0331452 0.5933048 Yme1L1 YME1-like 1 (S. cerevisiae) 3560 +/- 193 4334 +/- 244 3653 +/- 205 4168 +/- 128 -0.8001904 0.0331531 0.0657470 Pfkl phosphofructokinase, liver 1784 +/- 148 1890 +/- 147 1627 +/- 63 1907 +/- 88 -0.8612850 0.6198749 0.0337229 Wnt5A wingless-type MMTV integration site family, member 5A 485 +/- 51 763 +/- 107 574 +/- 90 877 +/- 81 0.2343027 0.0504035 0.0341374 Uqcrc1 ubiquinol-cytochrome c reductase core protein I 5480 +/- 38 5588 +/- 258 5191 +/- 86 5734 +/- 178 -0.4680811 0.6955350 0.0344393 Txnl1 thioredoxin-like 1 2413 +/- 328 2952 +/- 186 3020 +/- 129 3692 +/- 218 0.0116165 0.1909979 0.0344896 Lnpep leucyl/cystinyl aminopeptidase 2120 +/- 127 2540 +/- 116 2687 +/- 122 2808 +/- 110 0.0035853 0.0351572 0.4785739 Chm choroideremia (Rab escort protein 1) 1904 +/- 130 2177 +/- 95 1862 +/- 132 2272 +/- 98 0.9895516 0.1232673 0.0354721 Pdgfa platelet-derived growth factor alpha polypeptide 71 +/- 16 134 +/- 20 87 +/- 13 170 +/- 42 0.2924605 0.0358641 0.1192919 Mxi1 MAX interactor 1 6342 +/- 614 7312 +/- 652 4531 +/- 138 5071 +/- 166 -0.0005043 0.3040709 0.0362927 Eif5B eukaryotic translation initiation factor 5B 746 +/- 19 848 +/- 36 734 +/- 20 791 +/- 15 -0.2524863 0.0371269 0.0522234 Stat5B signal transducer and activator of transcription 5B 521 +/- 41 647 +/- 33 390 +/- 30 402 +/- 19 -0.0001616 0.0378386 0.7305091 Lyk5 protein kinase LYK5 1182 +/- 41 1209 +/- 34 1201 +/- 21 1266 +/- 17 0.3933912 0.6225568 0.0388311 Ogg1 8-oxoguanine DNA glycosylase 301 +/- 28 351 +/- 24 298 +/- 15 364 +/- 22 0.7001058 0.2095177 0.0394676 Dbt dihydrolipoamide branched chain transacylase E2 462 +/- 33 608 +/- 51 504 +/- 30 557 +/- 59 -0.4121075 0.0398338 0.4561002 Serpinb6 serpin peptidase inhibitor, clade B (ovalbumin), member 6 139 +/- 16 197 +/- 19 232 +/- 20 249 +/- 10 0.0035434 0.0405549 0.4693888 Mkln1 muskelin 1, intracellular mediator containing kelch motifs 727 +/- 42 901 +/- 60 770 +/- 27 780 +/- 19 -0.5074141 0.0406878 0.7599058 Cdc25A cell division cycle 25A 235 +/- 35 330 +/- 18 224 +/- 25 264 +/- 25 -0.0418073 0.0419309 0.2859052 Kidins220 Kinase D-interacting substance of 220 kDa 221 +/- 98 520 +/- 82 208 +/- 72 376 +/- 75 -0.0339253 0.0420293 0.1420246 Arrb1 arrestin, beta 1 380 +/- 17 456 +/- 27 349 +/- 27 389 +/- 28 -0.1563952 0.0424103 0.3310625 Rpl30 ribosomal protein L30 15248 +/- 656 16050 +/- 750 15243 +/- 600 17322 +/- 644 0.2488685 0.4397322 0.0433302 Nupl1 nucleoporin like 1 80 +/- 12 133 +/- 19 138 +/- 7 175 +/- 24 0.0003569 0.0436761 0.2121140 Apbb1 amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) 5027 +/- 303 5203 +/- 381 4570 +/- 115 4883 +/- 59 -0.1535392 0.7245922 0.0441443 Man2A1 Mannosidase, alpha, class 2A, member 1 1559 +/- 217 2028 +/- 286 855 +/- 94 1116 +/- 57 -0.0005909 0.2219283 0.0442734 Edg7 Endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 906 +/- 85 1108 +/- 56 920 +/- 115 1256 +/- 85 0.2705779 0.0789611 0.0446720 Srd5A1 steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4 264 +/- 147 539 +/- 62 255 +/- 133 609 +/- 56 0.5887142 0.1303820 0.0447201 Taldo1 transaldolase 1 955 +/- 59 1652 +/- 264 1013 +/- 64 1727 +/- 292 0.6311775 0.0454565 0.0696408

S3- Aging related genes in rhesus and rat.xls Page 4 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Slc26A2 solute carrier family 26 (sulfate transporter), member 2 87 +/- 10 217 +/- 49 135 +/- 50 223 +/- 61 0.4187154 0.0457219 0.2943412 Serpinf1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium d 508 +/- 96 868 +/- 124 280 +/- 49 579 +/- 121 -0.0018758 0.0462446 0.0678895 Nisch nischarin 262 +/- 23 343 +/- 27 377 +/- 20 423 +/- 44 0.0084632 0.0475203 0.3831798 Acy1 aminoacylase 1 493 +/- 28 523 +/- 20 435 +/- 30 520 +/- 22 -0.0727130 0.3972113 0.0487243 Down in Rhesus Aging Studies Only Strbp spermatid perinuclear RNA binding protein 926 +/- 84 754 +/- 29 785 +/- 30 663 +/- 42 -0.0375280 -0.0983040 -0.0493482 Rasgrf2 Ras protein-specific guanine nucleotide-releasing factor 2 335 +/- 22 273 +/- 17 423 +/- 53 365 +/- 12 0.0152537 -0.0481425 -0.3336087 Ubc ubiquitin C 6672 +/- 739 5772 +/- 426 7116 +/- 734 5191 +/- 278 -0.8565367 -0.3216693 -0.0472473 Gnb5 guanine nucleotide binding protein (G protein), beta 5 2978 +/- 211 2392 +/- 140 2607 +/- 211 2224 +/- 172 -0.1263201 -0.0465412 -0.1926185 Rac1 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding pro 4505 +/- 188 3402 +/- 520 4425 +/- 250 3002 +/- 507 -0.6489174 -0.0910153 -0.0459855 Myo1B myosin IB 240 +/- 40 180 +/- 30 189 +/- 25 121 +/- 13 -0.0197776 -0.2509192 -0.0456126 Syt11 synaptotagmin XI 2979 +/- 924 523 +/- 91 2747 +/- 804 738 +/- 96 -0.7192157 -0.0449002 -0.0544050 Ggtl3 gamma-glutamyltransferase-like 3 2529 +/- 133 2114 +/- 121 2837 +/- 156 2630 +/- 103 0.0017072 -0.0442082 -0.2981175 Arhgef7 Rho guanine nucleotide exchange factor (GEF) 7 3467 +/- 223 2866 +/- 102 2911 +/- 102 2790 +/- 61 -0.0283798 -0.0436735 -0.3363678 Sdhc succinate dehydrogenase complex, subunit C, integral membrane protein, 15 1763 +/- 91 1634 +/- 45 1610 +/- 52 1461 +/- 35 -0.0006076 -0.2429239 -0.0436667 Kcnh2 potassium voltage-gated channel, subfamily H (eag-related), member 2 579 +/- 45 458 +/- 18 529 +/- 26 467 +/- 29 -0.6410461 -0.0431321 -0.1478153 Phlda1 pleckstrin homology-like domain, family A, member 1 421 +/- 36 319 +/- 24 453 +/- 52 320 +/- 63 0.7121824 -0.0430721 -0.1408083 Pdgfb platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) o 468 +/- 21 394 +/- 23 454 +/- 37 444 +/- 37 0.7005402 -0.0429013 -0.8469609 Atp5D ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit 1719 +/- 90 1425 +/- 89 2012 +/- 103 1836 +/- 84 0.0005180 -0.0426828 -0.2155545 Gorasp2 golgi reassembly stacking protein 2, 55kDa 1888 +/- 69 1700 +/- 21 1893 +/- 58 1861 +/- 71 0.1220173 -0.0407395 -0.7377233 Casp6 Caspase 6, apoptosis-related cysteine peptidase 345 +/- 21 260 +/- 29 301 +/- 24 283 +/- 18 -0.2331043 -0.0403679 -0.5666451 Coro7 coronin 7 1060 +/- 57 830 +/- 78 1137 +/- 43 970 +/- 97 0.0534742 -0.0402191 -0.1709090 Garnl1 GTPase activating Rap/RanGAP domain-like 1 683 +/- 64 626 +/- 46 579 +/- 30 462 +/- 37 -0.0126510 -0.4858900 -0.0400986 Plk2 polo-like kinase 2 (Drosophila) 4576 +/- 519 3141 +/- 223 3989 +/- 267 3502 +/- 175 -0.7938381 -0.0397434 -0.1639475 Col11A2 collagen, type XI, alpha 2 262 +/- 34 231 +/- 38 308 +/- 19 229 +/- 25 0.5000081 -0.5567156 -0.0383340 Snf1Lk SNF1-like kinase 448 +/- 53 272 +/- 52 268 +/- 49 172 +/- 19 -0.0026891 -0.0381415 -0.1146252 Aplp2 Amyloid beta (A4) precursor-like protein 2 397 +/- 24 319 +/- 21 448 +/- 28 403 +/- 22 0.0107720 -0.0373294 -0.2404392 Pdcd6Ip programmed cell death 6 interacting protein 1108 +/- 45 997 +/- 36 1209 +/- 22 1087 +/- 41 0.0058318 -0.0841336 -0.0372195 Slc25A25 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 1121 +/- 61 974 +/- 52 1300 +/- 67 1101 +/- 43 0.0285995 -0.0967483 -0.0367214 Grina glutamate receptor, ionotropic, N-methyl D-asparate-associated protein 1 (gl 5549 +/- 298 4625 +/- 234 6955 +/- 300 6870 +/- 234 0.0000245 -0.0360443 -0.8289728 Itga8 integrin, alpha 8 101 +/- 17 101 +/- 14 126 +/- 12 92 +/- 3 0.1331249 -0.9822653 -0.0359952 Hmgcs2 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) 244 +/- 51 182 +/- 18 480 +/- 52 240 +/- 77 0.0158673 -0.2926355 -0.0352664 Pparg Peroxisome proliferative activated receptor, gamma 118 +/- 5 118 +/- 16 156 +/- 13 117 +/- 8 0.1182899 -0.9774051 -0.0350082 Ikbkap inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex 3512 +/- 159 3207 +/- 197 3071 +/- 108 2708 +/- 98 -0.0001912 -0.2572085 -0.0342733 Pex14 peroxisomal biogenesis factor 14 1046 +/- 74 925 +/- 41 1172 +/- 79 945 +/- 26 0.2574630 -0.1903719 -0.0341549 Cd5 CD5 molecule 143 +/- 6 90 +/- 18 125 +/- 19 122 +/- 17 0.7027175 -0.0340309 -0.9064233 Dctn4 dynactin 4 (p62) 2167 +/- 114 1786 +/- 115 2057 +/- 72 1769 +/- 87 -0.2351288 -0.0395917 -0.0338534 Pex6 peroxisomal biogenesis factor 6 1024 +/- 62 855 +/- 43 1081 +/- 68 886 +/- 19 0.2627287 -0.0513789 -0.0336719 Kcnip1 Kv channel interacting protein 1 233 +/- 13 189 +/- 12 243 +/- 17 208 +/- 14 0.4977055 -0.0334199 -0.1487670 Timm8A translocase of inner mitochondrial membrane 8 homolog A (yeast) 203 +/- 12 163 +/- 10 217 +/- 9 201 +/- 18 0.1477676 -0.0326712 -0.4626918 Htr3A 5-hydroxytryptamine (serotonin) receptor 3A 166 +/- 21 103 +/- 13 182 +/- 25 126 +/- 12 0.0452838 -0.0323096 -0.0784015 Tm9Sf2 transmembrane 9 superfamily member 2 1860 +/- 104 971 +/- 362 1861 +/- 69 746 +/- 349 -0.7211977 -0.0573688 -0.0315880 Egln1 egl nine homolog 1 (C. elegans) 599 +/- 67 426 +/- 24 799 +/- 81 561 +/- 31 0.0132782 -0.0500585 -0.0314922 Trpm7 transient receptor potential cation channel, subfamily M, member 7 356 +/- 40 334 +/- 21 378 +/- 30 285 +/- 20 -0.8708571 -0.6291703 -0.0314346 Acp1 acid phosphatase 1, soluble 2644 +/- 165 2074 +/- 157 2357 +/- 91 2031 +/- 147 -0.0852981 -0.0313063 -0.1018205 Dffa DNA fragmentation factor, 45kDa, alpha polypeptide 632 +/- 34 537 +/- 27 694 +/- 27 593 +/- 28 0.0281539 -0.0543054 -0.0312074 Kpna1 karyopherin alpha 1 (importin alpha 5) 2311 +/- 102 1982 +/- 79 2132 +/- 68 2092 +/- 86 -0.3460581 -0.0303221 -0.7251541 Tmf1 TATA element modulatory factor 1 2030 +/- 133 1560 +/- 130 2148 +/- 200 1684 +/- 181 0.2471757 -0.0295354 -0.1188377 Bzw1 basic leucine zipper and W2 domains 1 5216 +/- 116 4624 +/- 192 4100 +/- 120 3941 +/- 258 -0.0002480 -0.0288959 -0.5968530 Tcf4 transcription factor 4 4852 +/- 158 4083 +/- 243 6014 +/- 463 4769 +/- 717 0.0342518 -0.0276142 -0.1877160 Pnck pregnancy upregulated non-ubiquitously expressed CaM kinase 583 +/- 36 459 +/- 43 769 +/- 37 629 +/- 37 0.0005653 -0.0520961 -0.0263864 Crebbp CREB binding protein (Rubinstein-Taybi syndrome) 1537 +/- 76 1340 +/- 29 1670 +/- 25 1368 +/- 90 0.0746315 -0.0477222 -0.0261380 Kcnq2 potassium voltage-gated channel, KQT-like subfamily, member 2 279 +/- 12 234 +/- 12 261 +/- 17 237 +/- 14 -0.7567866 -0.0255233 -0.2864954 P2Rx5 purinergic receptor P2X, ligand-gated ion channel, 5 1338 +/- 74 1083 +/- 93 1528 +/- 87 1238 +/- 63 0.0259930 -0.0581722 -0.0255109 Akt3 V-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) 4120 +/- 100 3342 +/- 250 3311 +/- 184 3045 +/- 90 -0.0192162 -0.0251429 -0.2342718 Fndc5 fibronectin type III domain containing 5 519 +/- 27 422 +/- 25 470 +/- 26 433 +/- 42 -0.6271012 -0.0247525 -0.4894345 Ptbp2 polypyrimidine tract binding protein 2 2307 +/- 103 1782 +/- 161 2298 +/- 39 1811 +/- 152 0.9890389 -0.0238185 -0.0308303 Acvr1B activin A receptor, type IB 1767 +/- 140 1532 +/- 52 1675 +/- 66 1338 +/- 98 -0.1179970 -0.1633812 -0.0237609 Arhgef11 Rho guanine nucleotide exchange factor (GEF) 11 658 +/- 70 564 +/- 45 969 +/- 43 769 +/- 57 0.0005791 -0.2902121 -0.0236981 Doc2B double C2-like domains, beta 258 +/- 27 166 +/- 20 253 +/- 19 182 +/- 20 0.9949891 -0.0235749 -0.0301955

S3- Aging related genes in rhesus and rat.xls Page 5 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Kcnk2 potassium channel, subfamily K, member 2 130 +/- 38 100 +/- 18 109 +/- 11 75 +/- 3 -0.2321199 -0.4837929 -0.0227702 Cryba2 crystallin, beta A2 197 +/- 9 153 +/- 13 189 +/- 23 170 +/- 6 0.9023736 -0.0227566 -0.4580255 Ogt O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosam 1094 +/- 60 899 +/- 25 1800 +/- 83 1694 +/- 67 0.0000004 -0.0221208 -0.3487604 Cacnb1 calcium channel, voltage-dependent, beta 1 subunit 1084 +/- 79 825 +/- 44 1214 +/- 70 1044 +/- 36 0.0095231 -0.0219788 -0.0658955 Smu1 Smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) 1021 +/- 44 845 +/- 49 1000 +/- 21 892 +/- 31 0.7566227 -0.0241009 -0.0215579 Myadm myeloid-associated differentiation marker 444 +/- 27 337 +/- 28 548 +/- 77 393 +/- 23 0.0648073 -0.0207015 -0.1044596 Mlh1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) 569 +/- 45 480 +/- 41 470 +/- 32 363 +/- 16 -0.0051932 -0.1749710 -0.0198928 Max MYC associated factor X 1031 +/- 87 779 +/- 39 1103 +/- 54 911 +/- 39 0.0983481 -0.0342138 -0.0195643 Hspe1 heat shock 10kDa protein 1 (chaperonin 10) 3558 +/- 135 3064 +/- 138 3300 +/- 90 2930 +/- 94 -0.0544537 -0.0282547 -0.0194695 Nrxn1 neurexin 1 10862 +/- 245 9499 +/- 402 10764 +/- 142 9886 +/- 287 0.7704651 -0.0193078 -0.0339303 Wdfy1 WD repeat and FYVE domain containing 1 1082 +/- 175 808 +/- 190 857 +/- 69 579 +/- 68 -0.0155882 -0.3162518 -0.0186316 Vapa VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa 11082 +/- 381 9798 +/- 151 10132 +/- 197 8992 +/- 317 -0.0110698 -0.0180844 -0.0187738 Ucn urocortin 399 +/- 28 387 +/- 22 380 +/- 17 317 +/- 13 -0.1100041 -0.7410435 -0.0179206 Pik3R2 phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta) 5364 +/- 460 3833 +/- 348 4458 +/- 93 3892 +/- 156 -0.3389194 -0.0256077 -0.0177811 Hes5 hairy and enhancer of split 5 (Drosophila) 714 +/- 104 558 +/- 31 840 +/- 53 603 +/- 61 0.1479058 -0.2015184 -0.0177293 Rheb Ras homolog enriched in brain 7133 +/- 176 6424 +/- 174 6526 +/- 211 6223 +/- 272 -0.0979010 -0.0167407 -0.4047302 Fat3 FAT tumor suppressor homolog 3 (Drosophila) 802 +/- 78 532 +/- 31 691 +/- 50 625 +/- 62 -0.8582295 -0.0163774 -0.4303413 Sox11 SRY (sex determining region Y)-box 11 664 +/- 52 459 +/- 46 303 +/- 32 221 +/- 13 -0.0000094 -0.0145985 -0.0487541 Sec61A1 Sec61 alpha 1 subunit (S. cerevisiae) 320 +/- 43 269 +/- 28 351 +/- 21 276 +/- 12 0.6510648 -0.3404193 -0.0145461 Bicd2 bicaudal D homolog 2 (Drosophila) 1123 +/- 23 818 +/- 85 1051 +/- 110 902 +/- 129 0.0566063 -0.0144352 -0.4047251 Ptms parathymosin 564 +/- 27 451 +/- 59 772 +/- 43 611 +/- 28 0.0017468 -0.1244597 -0.0131330 Camkk2 calcium/calmodulin-dependent protein kinase kinase 2, beta 2835 +/- 306 2109 +/- 116 2217 +/- 96 1716 +/- 125 -0.0240822 -0.0657120 -0.0129466 Cttnbp2 cortactin binding protein 2 537 +/- 51 438 +/- 37 539 +/- 47 360 +/- 32 -0.3629991 -0.1546185 -0.0124030 Mapre3 microtubule-associated protein, RP/EB family, member 3 3330 +/- 90 2945 +/- 89 3841 +/- 102 3654 +/- 41 0.0000079 -0.0122431 -0.1344417 Nog Noggin 599 +/- 81 458 +/- 67 733 +/- 50 542 +/- 33 0.0513269 -0.2089942 -0.0119141 Skiv2L superkiller viralicidic activity 2-like (S. cerevisiae) 624 +/- 40 474 +/- 21 631 +/- 36 490 +/- 27 0.5380196 -0.0113638 -0.0121907 Pnma1 paraneoplastic antigen MA1 10959 +/- 391 9451 +/- 261 10858 +/- 318 10579 +/- 360 0.2885476 -0.0112107 -0.5770706 Baalc brain and acute leukemia, cytoplasmic 5677 +/- 195 4724 +/- 234 5418 +/- 180 4924 +/- 267 -0.9717174 -0.0110503 -0.1674274 Fads3 fatty acid desaturase 3 1186 +/- 74 995 +/- 86 1240 +/- 69 944 +/- 62 0.7204854 -0.1234827 -0.0109791 Scye1 small inducible cytokine subfamily E, member 1 (endothelial monocyte-activa 1313 +/- 43 1283 +/- 31 1364 +/- 27 1259 +/- 16 0.5242370 -0.5850455 -0.0098649 Prelp proline/arginine-rich end leucine-rich repeat protein 1546 +/- 52 1157 +/- 104 1515 +/- 84 1157 +/- 70 -0.6850686 -0.0116289 -0.0098213 Rapgef1 Rap guanine nucleotide exchange factor (GEF) 1 616 +/- 50 396 +/- 101 775 +/- 26 416 +/- 82 0.0433485 -0.0915843 -0.0095002 Zwint ZW10 interactor 592 +/- 49 356 +/- 55 462 +/- 44 332 +/- 21 -0.1055223 -0.0093248 -0.0321759 Suox sulfite oxidase 266 +/- 15 222 +/- 19 301 +/- 8 220 +/- 19 0.2424594 -0.1004053 -0.0088787 Cdipt CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol 3358 +/- 206 3149 +/- 168 3343 +/- 117 2839 +/- 95 -0.4056603 -0.4511265 -0.0087776 Psma6 proteasome (prosome, macropain) subunit, alpha type, 6 5154 +/- 216 4141 +/- 221 4713 +/- 143 4252 +/- 182 -0.3569863 -0.0083183 -0.0815815 Hes1 hairy and enhancer of split 1, (Drosophila) 395 +/- 33 353 +/- 23 461 +/- 26 352 +/- 18 0.1136724 -0.3270469 -0.0080258 Pcsk2 proprotein convertase subtilisin/kexin type 2 3146 +/- 220 2328 +/- 198 2601 +/- 119 2049 +/- 105 -0.0135462 -0.0204179 -0.0069513 Mark2 MAP/microtubule affinity-regulating kinase 2 823 +/- 33 702 +/- 30 941 +/- 40 765 +/- 25 0.0225006 -0.0230355 -0.0057067 Cacna1A calcium channel, voltage-dependent, P/Q type, alpha 1A subunit 961 +/- 89 664 +/- 44 1617 +/- 110 993 +/- 119 0.0001939 -0.0186447 -0.0041932 Idh3B isocitrate dehydrogenase 3 (NAD+) beta 1428 +/- 48 1204 +/- 29 1103 +/- 38 1036 +/- 36 -0.0001105 -0.0037977 -0.2334142 Prps2 phosphoribosyl pyrophosphate synthetase 2 1966 +/- 106 1443 +/- 29 1604 +/- 58 1369 +/- 46 -0.0207095 -0.0035250 -0.0119785 Brd2 bromodomain containing 2 683 +/- 52 469 +/- 53 945 +/- 41 572 +/- 72 0.0046808 -0.0165249 -0.0034740 Mgll monoglyceride lipase 886 +/- 65 790 +/- 40 920 +/- 52 666 +/- 35 -0.4728268 -0.2405204 -0.0033626 Mcf2L MCF.2 cell line derived transforming sequence-like 169 +/- 11 113 +/- 10 232 +/- 24 135 +/- 8 0.0084632 -0.0033328 -0.0076132 Snap25 Synaptosomal-associated protein, 25kDa 583 +/- 70 304 +/- 41 826 +/- 34 518 +/- 59 0.0011821 -0.0084664 -0.0032100 Timm10 translocase of inner mitochondrial membrane 10 homolog (yeast) 1629 +/- 120 1047 +/- 77 1208 +/- 47 967 +/- 68 -0.0198704 -0.0031533 -0.0212383 Usp15 ubiquitin specific peptidase 15 2158 +/- 53 1639 +/- 107 2009 +/- 51 1697 +/- 92 -0.2965901 -0.0031348 -0.0232526 Plaa phospholipase A2-activating protein 952 +/- 29 788 +/- 29 921 +/- 43 883 +/- 20 0.5396908 -0.0023769 -0.4435661 Gpm6A glycoprotein M6A 20299 +/- 453 16530 +/- 744 18774 +/- 263 16875 +/- 764 -0.1436309 -0.0023422 -0.0660502 Kcna5 potassium voltage-gated channel, shaker-related subfamily, member 5 160 +/- 33 140 +/- 13 180 +/- 11 124 +/- 7 0.9269433 -0.5818771 -0.0023091 Set SET translocation (myeloid leukemia-associated) 698 +/- 22 583 +/- 14 1015 +/- 52 866 +/- 35 0.0000002 -0.0021928 -0.0424203 Socs1 suppressor of cytokine signaling 1 193 +/- 12 114 +/- 15 214 +/- 14 159 +/- 18 0.0011964 -0.0021482 -0.0385425 Rtn3 reticulon 3 21006 +/- 1230 13544 +/- 1159 20556 +/- 206 13859 +/- 1090 -0.8308618 -0.0013140 -0.0029463 Gchfr GTP cyclohydrolase I feedback regulator 417 +/- 27 263 +/- 11 403 +/- 32 315 +/- 18 0.4719246 -0.0012334 -0.0441217 Gabrg3 gamma-aminobutyric acid (GABA) A receptor, gamma 3 83 +/- 11 74 +/- 14 144 +/- 6 100 +/- 6 0.0009088 -0.5968885 -0.0007611 Ltbp1 latent transforming growth factor beta binding protein 1 361 +/- 17 236 +/- 20 447 +/- 38 218 +/- 28 0.1951325 -0.0007146 -0.0009587 Pum2 pumilio homolog 2 (Drosophila) 2633 +/- 77 1935 +/- 111 2753 +/- 131 1997 +/- 141 0.4111452 -0.0006045 -0.0036483 Shank2 SH3 and multiple ankyrin repeat domains 2 3124 +/- 137 2183 +/- 63 2942 +/- 130 2120 +/- 154 -0.4032327 -0.0004179 -0.0032766 Lrrn3 leucine rich repeat neuronal 3 1738 +/- 84 1547 +/- 86 1796 +/- 57 1379 +/- 50 -0.8735055 -0.1422784 -0.0003750

S3- Aging related genes in rhesus and rat.xls Page 6 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Znrd1 zinc ribbon domain containing 1 926 +/- 41 625 +/- 37 853 +/- 26 720 +/- 30 0.9403265 -0.0002687 -0.0099077 Up in Rat Aging Studies Only Ebp emopamil binding protein (sterol isomerase) 1051 +/- 62 1024 +/- 49 1079 +/- 36 1062 +/- 60 0.6436479 -0.7417843 -0.8184843 Aspa aspartoacylase (Canavan disease) 1618 +/- 355 1441 +/- 348 645 +/- 98 601 +/- 82 -0.0046134 -0.7297413 -0.7434847 Atp1A2 ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide 13537 +/- 634 13224 +/- 524 13312 +/- 436 13206 +/- 529 -0.9963926 -0.7118095 -0.8808246 Bet1L blocked early in transport 1 homolog (S. cerevisiae)-like 621 +/- 54 586 +/- 65 668 +/- 65 667 +/- 22 0.2985623 -0.6887562 -0.9926211 Rps26 ribosomal protein S26 13524 +/- 616 13145 +/- 731 11557 +/- 620 11245 +/- 406 -0.0000017 -0.6998089 -0.6844055 Prdx1 peroxiredoxin 1 107 +/- 8 101 +/- 8 113 +/- 12 106 +/- 9 0.6589474 -0.6361815 -0.6725031 Eef2K eukaryotic elongation factor-2 kinase 1581 +/- 177 1477 +/- 98 1721 +/- 108 1635 +/- 117 0.2001932 -0.6232192 -0.6018169 Igsf1 immunoglobulin superfamily, member 1 781 +/- 111 761 +/- 66 793 +/- 81 738 +/- 55 -0.8841248 -0.8824478 -0.5864280 Sort1 sortilin 1 796 +/- 70 720 +/- 113 917 +/- 106 914 +/- 77 0.2273917 -0.5817462 -0.9862295 Gfap glial fibrillary acidic protein 93 +/- 19 92 +/- 11 120 +/- 16 104 +/- 24 0.3535950 -0.9686178 -0.5813068 Ache acetylcholinesterase (Yt blood group) 247 +/- 28 246 +/- 21 390 +/- 22 370 +/- 26 0.0002706 -0.9668567 -0.5719788 Galc galactosylceramidase 844 +/- 48 808 +/- 51 817 +/- 49 750 +/- 95 -0.3583847 -0.6187456 -0.5570988 Rps17 ribosomal protein S17 15133 +/- 550 14659 +/- 548 12410 +/- 409 12221 +/- 313 -0.0000260 -0.5552325 -0.7220457 Clu clusterin 3878 +/- 541 3451 +/- 377 2851 +/- 426 2733 +/- 149 -0.0200961 -0.5332410 -0.8025165 Zfp36 zinc finger protein 36, C3H type, homolog (mouse) 562 +/- 20 558 +/- 20 562 +/- 13 533 +/- 37 -0.4668616 -0.8898812 -0.4906276 Dbnl drebrin-like 1945 +/- 80 1865 +/- 71 1998 +/- 76 1957 +/- 55 0.1274646 -0.4733026 -0.6765954 Epas1 endothelial PAS domain protein 1 3071 +/- 166 2921 +/- 93 2305 +/- 183 2256 +/- 84 -0.0000036 -0.4529431 -0.8135122 Grn granulin 1767 +/- 125 1735 +/- 78 1562 +/- 33 1507 +/- 52 -0.0117135 -0.8302307 -0.4027456 Cplx2 complexin 2 10282 +/- 1444 8795 +/- 869 9830 +/- 852 9803 +/- 400 0.5932191 -0.4025333 -0.9779180 Hadhb hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/e 914 +/- 38 909 +/- 85 750 +/- 24 687 +/- 61 -0.0001281 -0.9513261 -0.3784315 Dnajc5 DnaJ (Hsp40) homolog, subfamily C, member 5 2366 +/- 278 2055 +/- 187 3214 +/- 219 2977 +/- 149 0.0000445 -0.3771709 -0.3959412 Aadat aminoadipate aminotransferase 271 +/- 29 237 +/- 19 145 +/- 17 143 +/- 19 -0.0003348 -0.3705286 -0.9598542 Rab31 RAB31, member RAS oncogene family 3868 +/- 294 3537 +/- 160 3341 +/- 297 3197 +/- 162 -0.0596723 -0.3544097 -0.6818644 Gng8 guanine nucleotide binding protein (G protein), gamma 8 409 +/- 52 397 +/- 57 518 +/- 45 467 +/- 27 0.0071224 -0.8821712 -0.3536938 Bcas1 breast carcinoma amplified sequence 1 2055 +/- 301 1589 +/- 364 1783 +/- 208 1588 +/- 181 -0.4604672 -0.3476227 -0.4960115 Phldb1 pleckstrin homology-like domain, family B, member 1 246 +/- 32 207 +/- 20 159 +/- 23 156 +/- 37 -0.0398668 -0.3390269 -0.9632182 Cxcl12 chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) 703 +/- 160 527 +/- 166 1328 +/- 203 1073 +/- 141 0.0097847 -0.4640184 -0.3318161 Fhl1 four and a half LIM domains 1 4434 +/- 296 3835 +/- 494 6047 +/- 320 5973 +/- 158 0.0001980 -0.3280146 -0.8413964 Acsl3 acyl-CoA synthetase long-chain family member 3 2056 +/- 491 1593 +/- 214 2104 +/- 482 1547 +/- 203 0.8668591 -0.4158419 -0.3237906 Neurod1 neurogenic differentiation 1 3513 +/- 1010 2385 +/- 631 4285 +/- 253 3926 +/- 228 0.0490227 -0.3701073 -0.3204471 Synj1 synaptojanin 1 7756 +/- 393 7178 +/- 387 7982 +/- 449 7838 +/- 286 0.1676523 -0.3188251 -0.7931439 Acvr1C activin A receptor, type IC 504 +/- 117 394 +/- 115 1153 +/- 118 1015 +/- 47 0.0000267 -0.5174737 -0.3121776 Fbxl20 F-box and leucine-rich repeat protein 20 390 +/- 20 371 +/- 24 331 +/- 14 297 +/- 26 -0.0039374 -0.5661398 -0.3046508 Mapk12 mitogen-activated protein kinase 12 426 +/- 42 393 +/- 25 440 +/- 29 395 +/- 28 0.6847311 -0.5192586 -0.2947657 Eif4Ebp1 eukaryotic translation initiation factor 4E binding protein 1 362 +/- 23 361 +/- 37 292 +/- 13 256 +/- 28 -0.0010758 -0.9776054 -0.2943072 Galm galactose mutarotase (aldose 1-epimerase) 270 +/- 41 220 +/- 17 203 +/- 49 190 +/- 43 -0.1138736 -0.2938327 -0.8409153 Blcap bladder cancer associated protein 6297 +/- 214 5807 +/- 369 5335 +/- 115 5220 +/- 151 -0.0081694 -0.2842212 -0.5620694 Cited2 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal doma 371 +/- 56 291 +/- 43 432 +/- 47 407 +/- 51 0.1240745 -0.2829936 -0.7214939 Ak2 adenylate kinase 2 232 +/- 19 205 +/- 13 283 +/- 15 268 +/- 21 0.0012047 -0.2587292 -0.5733950 Ptma prothymosin, alpha (gene sequence 28) 2337 +/- 132 2228 +/- 259 2180 +/- 138 1932 +/- 148 -0.2410777 -0.7196411 -0.2511091 Podxl podocalyxin-like 1966 +/- 277 1641 +/- 107 1601 +/- 72 1461 +/- 87 -0.1040655 -0.3132247 -0.2484971 Sfrs5 splicing factor, arginine/serine-rich 5 233 +/- 46 174 +/- 13 258 +/- 29 215 +/- 18 0.1853768 -0.2657320 -0.2479777 Mpg N-methylpurine-DNA glycosylase 722 +/- 51 636 +/- 45 644 +/- 33 607 +/- 22 -0.0783427 -0.2341435 -0.3733852 Nr1H2 nuclear receptor subfamily 1, group H, member 2 848 +/- 64 838 +/- 63 795 +/- 32 722 +/- 43 -0.1682361 -0.9116841 -0.2114146 Sdc3 syndecan 3 (N-syndecan) 3986 +/- 175 3565 +/- 247 3931 +/- 74 3858 +/- 235 0.2787272 -0.1972956 -0.7802691 Rpl41 Ribosomal protein L41 2686 +/- 132 2481 +/- 156 2471 +/- 52 2345 +/- 71 -0.1651898 -0.3422815 -0.1940890 Fn1 fibronectin 1 1193 +/- 123 1012 +/- 110 905 +/- 96 749 +/- 48 -0.0010688 -0.2989742 -0.1899981 Rapgef3 Rap guanine nucleotide exchange factor (GEF) 3 432 +/- 74 415 +/- 48 358 +/- 20 326 +/- 10 -0.0979008 -0.8546773 -0.1801893 Il6St Interleukin 6 signal transducer (gp130, oncostatin M receptor) 119 +/- 7 94 +/- 15 122 +/- 13 90 +/- 23 0.9931486 -0.1751611 -0.2737672 Dck deoxycytidine kinase 498 +/- 49 406 +/- 39 523 +/- 24 507 +/- 25 0.1616639 -0.1712138 -0.6588035 Galnt1 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltra 1487 +/- 76 1307 +/- 95 1286 +/- 57 1262 +/- 72 -0.0434836 -0.1712013 -0.8031009 Tpmt thiopurine S-methyltransferase 634 +/- 112 427 +/- 68 643 +/- 125 460 +/- 55 0.3263324 -0.1533641 -0.2212500 Pdp2 Pyruvate dehydrogenase phosphatase isoenzyme 2 929 +/- 58 889 +/- 19 897 +/- 35 820 +/- 34 -0.1380455 -0.5325371 -0.1495255 Fst follistatin 144 +/- 19 122 +/- 15 243 +/- 50 155 +/- 19 0.0244298 -0.3908552 -0.1477706 Nppa natriuretic peptide precursor A 1453 +/- 633 764 +/- 148 557 +/- 90 356 +/- 83 -0.1076100 -0.3333928 -0.1357757 Uxs1 UDP-glucuronate decarboxylase 1 801 +/- 71 662 +/- 61 974 +/- 34 857 +/- 59 0.0017190 -0.1702266 -0.1338492 Scp2 Sterol carrier protein 2 687 +/- 54 604 +/- 26 740 +/- 39 654 +/- 35 0.3488124 -0.2108697 -0.1329085 Hbb hemoglobin, beta 8460 +/- 2004 4805 +/- 635 4797 +/- 1700 4794 +/- 780 -0.0163883 -0.1327314 -0.9987740

S3- Aging related genes in rhesus and rat.xls Page 7 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Herpud1 homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-lik 3426 +/- 201 3045 +/- 102 3151 +/- 116 3068 +/- 66 -0.2347543 -0.1318209 -0.5535816 Egfr Epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) onco 196 +/- 15 157 +/- 18 185 +/- 16 155 +/- 12 -0.6933144 -0.1296721 -0.1567750 Homer3 homer homolog 3 (Drosophila) 250 +/- 55 168 +/- 32 356 +/- 16 267 +/- 46 0.0429181 -0.2357537 -0.1271272 Csda cold shock domain protein A 681 +/- 100 532 +/- 69 518 +/- 36 450 +/- 14 -0.1019928 -0.2538941 -0.1266500 Mapk1 mitogen-activated protein kinase 1 10274 +/- 704 8702 +/- 613 10851 +/- 475 10382 +/- 169 0.0274736 -0.1235692 -0.3873025 Slc38A2 Solute carrier family 38, member 2 139 +/- 18 127 +/- 8 166 +/- 24 119 +/- 9 0.6003279 -0.5704620 -0.1224705 Pcna proliferating cell nuclear antigen 1009 +/- 49 887 +/- 53 1030 +/- 12 998 +/- 73 0.2257035 -0.1209201 -0.6891272 Pscd2 pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2) 1012 +/- 49 896 +/- 46 1070 +/- 31 1034 +/- 25 0.0145388 -0.1157200 -0.4026028 Kcnn1 potassium intermediate/small conductance calcium-activated channel, subfa 1284 +/- 146 953 +/- 120 1350 +/- 85 1296 +/- 62 0.0797488 -0.1117496 -0.6188773 Pam peptidylglycine alpha-amidating monooxygenase 9384 +/- 821 7664 +/- 510 6095 +/- 300 5478 +/- 570 -0.0003074 -0.1114771 -0.3740806 Cyba cytochrome b-245, alpha polypeptide 1308 +/- 102 1275 +/- 108 1067 +/- 80 888 +/- 62 -0.0023596 -0.8243239 -0.1103755 Slc9A3 Solute carrier family 9 (sodium/hydrogen exchanger), member 3 215 +/- 18 177 +/- 1 195 +/- 21 176 +/- 16 -0.3643239 -0.0940914 -0.4928612 Ensa endosulfine alpha 8616 +/- 458 6910 +/- 758 8136 +/- 370 6992 +/- 536 -0.6244127 -0.0891463 -0.1204346 Ednrb endothelin receptor type B 673 +/- 94 582 +/- 62 937 +/- 97 716 +/- 58 0.0078100 -0.4398319 -0.0872201 Mertk c-mer proto-oncogene tyrosine kinase 700 +/- 45 687 +/- 47 695 +/- 34 609 +/- 27 -0.6064318 -0.8486662 -0.0837144 Nfix nuclear factor I/X (CCAAT-binding transcription factor) 215 +/- 16 195 +/- 13 347 +/- 26 287 +/- 15 0.0002591 -0.3584218 -0.0833231 Mapt microtubule-associated protein tau 6978 +/- 252 6298 +/- 237 7144 +/- 345 6697 +/- 249 0.2188318 -0.0781682 -0.3222767 Prkab1 protein kinase, AMP-activated, beta 1 non-catalytic subunit 182 +/- 22 157 +/- 14 201 +/- 9 161 +/- 17 0.5403512 -0.3502186 -0.0746359 Tob1 transducer of ERBB2, 1 172 +/- 34 132 +/- 24 260 +/- 18 168 +/- 38 0.0347507 -0.3564328 -0.0745297 Rod1 ROD1 regulator of differentiation 1 (S. pombe) 1784 +/- 98 1541 +/- 71 1943 +/- 113 1743 +/- 34 0.0110373 -0.0740837 -0.1424573 Cdh2 cadherin 2, type 1, N-cadherin (neuronal) 810 +/- 57 761 +/- 34 909 +/- 44 800 +/- 28 0.0426973 -0.4805146 -0.0675224 Tgfbi transforming growth factor, beta-induced, 68kDa 198 +/- 33 110 +/- 12 75 +/- 20 58 +/- 9 -0.0194205 -0.0674671 -0.4663408 Adra2C adrenergic, alpha-2C-, receptor 433 +/- 40 332 +/- 23 364 +/- 33 304 +/- 35 -0.2371924 -0.0599996 -0.2482227 Lasp1 LIM and SH3 protein 1 3715 +/- 152 3291 +/- 108 3992 +/- 179 3563 +/- 143 0.1229790 -0.0486642 -0.0948552 Slc12A5 solute carrier family 12, (potassium-chloride transporter) member 5 9260 +/- 395 7923 +/- 381 8510 +/- 287 7677 +/- 214 -0.1625091 -0.0351655 -0.0457884 Sts steroid sulfatase (microsomal), arylsulfatase C, isozyme S 522 +/- 29 428 +/- 25 424 +/- 42 346 +/- 14 -0.0213875 -0.0333906 -0.1245938 Rpl31 ribosomal protein L31 232 +/- 31 172 +/- 17 275 +/- 9 198 +/- 25 0.0482206 -0.1283849 -0.0329356 Nfil3 nuclear factor, interleukin 3 regulated 495 +/- 40 462 +/- 38 822 +/- 51 669 +/- 29 0.0007822 -0.5628783 -0.0322917 Robo2 roundabout, axon guidance receptor, homolog 2 (Drosophila) 1844 +/- 48 1630 +/- 69 1649 +/- 73 1537 +/- 82 -0.0304645 -0.0321743 -0.3379253 Csnk1D casein kinase 1, delta 809 +/- 36 702 +/- 20 866 +/- 60 764 +/- 92 0.2643752 -0.0313341 -0.3867637 Tpt1 tumor protein, translationally-controlled 1 440 +/- 33 330 +/- 27 527 +/- 42 469 +/- 48 0.0017671 -0.0285349 -0.3851388 Tgm2 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransf 141 +/- 8 82 +/- 19 137 +/- 20 132 +/- 28 0.2640993 -0.0261351 -0.9007881 Fut1 Fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, H blood gro 147 +/- 13 121 +/- 9 139 +/- 12 91 +/- 13 -0.1229487 -0.1360864 -0.0250978 Tgfb3 transforming growth factor, beta 3 505 +/- 61 393 +/- 48 651 +/- 37 486 +/- 46 0.0012308 -0.1826780 -0.0228507 Pros1 protein S (alpha) 774 +/- 108 426 +/- 28 473 +/- 63 375 +/- 23 -0.0440593 -0.0221690 -0.1904530 Rpl6 ribosomal protein L6 10615 +/- 183 9845 +/- 191 10876 +/- 273 9843 +/- 437 0.3850185 -0.0155943 -0.0857000 Echdc1 enoyl Coenzyme A hydratase domain containing 1 1796 +/- 60 1599 +/- 72 1651 +/- 62 1404 +/- 53 -0.0030410 -0.0614275 -0.0137135 Masp1 mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra 248 +/- 14 181 +/- 18 233 +/- 11 210 +/- 10 0.4003435 -0.0136085 -0.1571761 Acadl acyl-Coenzyme A dehydrogenase, long chain 234 +/- 12 184 +/- 12 226 +/- 9 179 +/- 19 -0.3592968 -0.0135989 -0.0654766 Csnk1G2 casein kinase 1, gamma 2 464 +/- 48 332 +/- 27 622 +/- 27 496 +/- 28 0.0018629 -0.0446968 -0.0104091 Cd47 CD47 molecule 2438 +/- 231 1877 +/- 56 2572 +/- 107 2131 +/- 78 0.0686341 -0.0595074 -0.0091856 Matr3 matrin 3 18535 +/- 672 15789 +/- 403 18512 +/- 661 16674 +/- 807 0.5064254 -0.0077536 -0.1152625 Rad50 RAD50 homolog (S. cerevisiae) 416 +/- 27 289 +/- 24 390 +/- 21 293 +/- 18 -0.3483701 -0.0053463 -0.0068159 Rpl15 Ribosomal protein L15 201 +/- 25 146 +/- 14 188 +/- 13 126 +/- 10 -0.2369957 -0.0919435 -0.0051823 Rps12 ribosomal protein S12 4329 +/- 211 3150 +/- 239 3422 +/- 186 2508 +/- 191 -0.0002343 -0.0042185 -0.0076363 Spa17 sperm autoantigenic protein 17 537 +/- 65 330 +/- 45 443 +/- 23 329 +/- 17 -0.5063023 -0.0275973 -0.0033444 Grm5 Glutamate receptor, metabotropic 5 4099 +/- 219 3135 +/- 171 3523 +/- 175 2644 +/- 98 -0.0543625 -0.0065106 -0.0025904 Vegf vascular endothelial growth factor 2713 +/- 90 2084 +/- 104 2439 +/- 134 2150 +/- 54 -0.4078724 -0.0010854 -0.0888537 Cd63 CD63 molecule 3961 +/- 565 4903 +/- 297 3173 +/- 139 3664 +/- 165 -0.0110740 0.1803626 0.0509958 Litaf lipopolysaccharide-induced TNF factor 1951 +/- 250 2975 +/- 472 1460 +/- 69 1769 +/- 112 -0.0149956 0.0932603 0.0527648 Renbp renin binding protein 106 +/- 14 148 +/- 12 134 +/- 15 152 +/- 8 0.0184974 0.0531180 0.3339102 Olig1 oligodendrocyte transcription factor 1 10505 +/- 1321 11917 +/- 1508 6389 +/- 320 7664 +/- 455 -0.0022764 0.4974144 0.0533656 Cspg2 chondroitin sulfate proteoglycan 2 (versican) 919 +/- 73 1111 +/- 44 1113 +/- 87 1200 +/- 59 0.1012591 0.0537952 0.4315227 Bin1 bridging integrator 1 3287 +/- 261 3907 +/- 355 3154 +/- 108 3522 +/- 125 -0.2615745 0.1924902 0.0547158 Cd48 CD48 molecule 394 +/- 43 577 +/- 75 274 +/- 24 387 +/- 42 -0.0039038 0.0660649 0.0551950 Ppap2C phosphatidic acid phosphatase type 2C 847 +/- 150 1265 +/- 223 430 +/- 69 630 +/- 61 -0.0065357 0.1548890 0.0576590 Clic1 chloride intracellular channel 1 450 +/- 32 468 +/- 12 341 +/- 23 402 +/- 15 -0.0000393 0.6054770 0.0583284 Lamp1 lysosomal-associated membrane protein 1 4569 +/- 324 5261 +/- 423 3749 +/- 165 4315 +/- 199 -0.0193457 0.2254370 0.0591777 Nfe2L2 nuclear factor (erythroid-derived 2)-like 2 2479 +/- 133 3346 +/- 356 1953 +/- 113 2015 +/- 87 -0.0045017 0.0599949 0.6718247 Rps9 Ribosomal protein S9 2793 +/- 217 3319 +/- 103 2565 +/- 81 2814 +/- 257 -0.0861802 0.0638984 0.3998186

S3- Aging related genes in rhesus and rat.xls Page 8 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG S100A4 S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, m 129 +/- 16 182 +/- 19 118 +/- 9 146 +/- 18 -0.0505237 0.0651834 0.1981630 Msn moesin 2073 +/- 132 2454 +/- 128 2123 +/- 63 2318 +/- 72 -0.6835327 0.0657167 0.0749129 Ctsd cathepsin D (lysosomal aspartyl peptidase) 96 +/- 9 129 +/- 13 113 +/- 15 163 +/- 20 0.0372739 0.0665338 0.0760518 Sh3Bp5 SH3-domain binding protein 5 (BTK-associated) 884 +/- 60 1084 +/- 77 999 +/- 36 1050 +/- 39 0.3357869 0.0690928 0.3628986 Gpx4 glutathione peroxidase 4 (phospholipid hydroperoxidase) 7963 +/- 464 8464 +/- 561 6679 +/- 145 7234 +/- 220 -0.0043258 0.5074510 0.0719936 Ncoa3 Nuclear receptor coactivator 3 89 +/- 15 124 +/- 9 166 +/- 12 180 +/- 18 0.0002706 0.0723115 0.5283242 Gpsm3 G-protein signalling modulator 3 (AGS3-like, C. elegans) 184 +/- 13 220 +/- 15 181 +/- 7 221 +/- 17 -0.8190921 0.1028015 0.0724839 Map1B microtubule-associated protein 1B 23091 +/- 887 25213 +/- 565 23220 +/- 887 24935 +/- 1214 -0.9070223 0.0763942 0.2886424 Klk6 kallikrein 6 (neurosin, zyme) 1030 +/- 190 1991 +/- 422 602 +/- 104 823 +/- 47 -0.0142445 0.0768100 0.0940555 Lipe lipase, hormone-sensitive 641 +/- 74 906 +/- 109 439 +/- 29 475 +/- 22 -0.0040681 0.0768589 0.3553490 Ntrk2 neurotrophic tyrosine kinase, receptor, type 2 10935 +/- 281 12204 +/- 576 11020 +/- 667 11737 +/- 475 -0.3476670 0.0868529 0.4047357 Dpp7 Dipeptidyl-peptidase 7 609 +/- 22 921 +/- 147 597 +/- 21 848 +/- 117 -0.0688070 0.0870566 0.0989640 Rhoa ras homolog gene family, member A 6057 +/- 234 6889 +/- 365 6013 +/- 192 6545 +/- 208 -0.3131028 0.0889958 0.0942288 Ptprf protein tyrosine phosphatase, receptor type, F 1938 +/- 116 2391 +/- 203 2131 +/- 38 2379 +/- 181 0.0671870 0.0891860 0.2479416 Prdx6 peroxiredoxin 6 5355 +/- 529 6504 +/- 257 6108 +/- 398 7058 +/- 298 0.0362312 0.0905172 0.0892078 Gpd1 glycerol-3-phosphate dehydrogenase 1 (soluble) 451 +/- 61 597 +/- 65 607 +/- 50 773 +/- 69 0.0458656 0.1314136 0.0894053 Pde11A phosphodiesterase 11A 104 +/- 10 172 +/- 32 86 +/- 7 103 +/- 21 -0.0648692 0.0932092 0.4813220 Ptpn6 protein tyrosine phosphatase, non-receptor type 6 277 +/- 7 305 +/- 17 299 +/- 11 327 +/- 10 0.0690073 0.1710919 0.0943146 Cd86 CD86 molecule 127 +/- 14 208 +/- 39 86 +/- 6 132 +/- 24 -0.0119421 0.0945678 0.1234214 Asrgl1 asparaginase like 1 1405 +/- 86 1591 +/- 49 1479 +/- 54 1618 +/- 167 0.6547981 0.0980042 0.4685318 Cryab crystallin, alpha B 11364 +/- 1007 13433 +/- 1482 8015 +/- 574 9478 +/- 561 -0.0062872 0.2787117 0.1018619 Pld1 phospholipase D1, phosphatidylcholine-specific 225 +/- 35 314 +/- 48 141 +/- 17 181 +/- 14 -0.0085037 0.1694986 0.1030327 Pde8A Phosphodiesterase 8A 844 +/- 229 1285 +/- 192 545 +/- 66 690 +/- 45 -0.0105159 0.1714711 0.1036090 Ptgds prostaglandin D2 synthase 21kDa (brain) 22511 +/- 2273 24357 +/- 1112 18690 +/- 1056 21104 +/- 809 -0.0064959 0.4885458 0.1036685 Eef1D Eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange 115 +/- 11 148 +/- 15 119 +/- 12 134 +/- 12 -0.5641078 0.1041495 0.4202773 Rpl19 ribosomal protein L19 9357 +/- 185 10005 +/- 306 8787 +/- 293 9412 +/- 306 -0.0021929 0.1062607 0.1740191 Rab13 RAB13, member RAS oncogene family 906 +/- 66 1051 +/- 48 935 +/- 61 1071 +/- 50 0.5919567 0.1084248 0.1152042 Bet1 BET1 homolog (S. cerevisiae) 629 +/- 39 713 +/- 26 683 +/- 35 689 +/- 9 0.6592954 0.1100186 0.8740623 Aldh6A1 aldehyde dehydrogenase 6 family, member A1 582 +/- 117 835 +/- 107 602 +/- 74 747 +/- 28 -0.7825405 0.1423545 0.1115908 Jag2 jagged 2 536 +/- 24 626 +/- 44 662 +/- 54 716 +/- 70 0.0159436 0.1127483 0.5633403 Alad aminolevulinate, delta-, dehydratase 192 +/- 24 250 +/- 23 165 +/- 13 200 +/- 16 -0.0451113 0.1145861 0.1279879 Psme1 proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) 1517 +/- 84 1712 +/- 77 1341 +/- 45 1539 +/- 108 -0.0050461 0.1185632 0.1476109 Tgfa transforming growth factor, alpha 114 +/- 13 154 +/- 25 140 +/- 23 188 +/- 15 0.2370484 0.1921067 0.1213186 Itpkb inositol 1,4,5-trisphosphate 3-kinase B 15463 +/- 1359 18302 +/- 973 16204 +/- 924 16683 +/- 494 -0.9729089 0.1234325 0.6603844 Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) 175 +/- 8 201 +/- 13 206 +/- 6 207 +/- 7 0.0387886 0.1254885 0.9269295 Ctsc cathepsin C 128 +/- 11 141 +/- 20 95 +/- 7 122 +/- 14 -0.0847890 0.5889993 0.1301646 Myo9B myosin IXB 669 +/- 94 844 +/- 90 446 +/- 24 519 +/- 35 -0.0018177 0.2050871 0.1318636 Alox5Ap arachidonate 5-lipoxygenase-activating protein 396 +/- 54 473 +/- 55 281 +/- 18 331 +/- 24 -0.0100841 0.3371905 0.1341690 Galnt7 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltra 507 +/- 38 593 +/- 37 358 +/- 16 368 +/- 28 -0.0001438 0.1347164 0.7518594 Slc12A2 solute carrier family 12 (sodium/potassium/chloride transporters), member 2 528 +/- 75 635 +/- 104 328 +/- 46 433 +/- 45 -0.0050464 0.4271903 0.1387237 Jund jun D proto-oncogene 679 +/- 56 787 +/- 57 912 +/- 63 1118 +/- 106 0.0008301 0.2023003 0.1403335 S100B S100 calcium binding protein, beta (neural) 123 +/- 20 174 +/- 25 143 +/- 22 179 +/- 40 0.6902436 0.1405708 0.4532374 Kctd10 potassium channel tetramerisation domain containing 10 234 +/- 64 309 +/- 28 239 +/- 54 348 +/- 40 0.2546901 0.3205415 0.1407388 Pbef1 pre-B-cell colony enhancing factor 1 1321 +/- 53 1436 +/- 49 1222 +/- 45 1355 +/- 80 -0.2117934 0.1443293 0.1926653 Lamp2 lysosomal-associated membrane protein 2 1002 +/- 127 1448 +/- 245 598 +/- 83 745 +/- 47 -0.0060799 0.1463506 0.1644174 Stxbp3 syntaxin binding protein 3 208 +/- 28 329 +/- 69 213 +/- 18 301 +/- 69 -0.8802035 0.1495316 0.2825708 Dnase2 deoxyribonuclease II, lysosomal 327 +/- 64 457 +/- 53 308 +/- 33 407 +/- 51 -0.2965691 0.1496835 0.1502204 Rps15 ribosomal protein S15 10399 +/- 364 11238 +/- 397 8679 +/- 352 9306 +/- 297 -0.0000130 0.1499550 0.2068656 Pmp22 Peripheral myelin protein 22 414 +/- 67 541 +/- 45 286 +/- 24 316 +/- 42 -0.0031605 0.1519767 0.5632738 Csrp1 cysteine and glycine-rich protein 1 10474 +/- 785 12051 +/- 791 7739 +/- 205 8522 +/- 429 -0.0007075 0.1872883 0.1530052 Sparcl1 SPARC-like 1 (mast9, hevin) 20455 +/- 810 21245 +/- 1040 19170 +/- 136 20139 +/- 549 -0.0251948 0.5630312 0.1545429 Laptm5 lysosomal associated multispanning membrane protein 5 499 +/- 54 666 +/- 93 552 +/- 59 639 +/- 82 0.9792278 0.1587406 0.4184732 Cirbp cold inducible RNA binding protein 1972 +/- 371 2621 +/- 206 1719 +/- 180 2040 +/- 99 -0.0910952 0.1661094 0.1593236 St18 suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) 1703 +/- 277 2524 +/- 453 1389 +/- 126 1471 +/- 154 -0.0729390 0.1595393 0.6898929 Gatm glycine amidinotransferase (L-arginine:glycine amidinotransferase) 3776 +/- 286 4611 +/- 460 3996 +/- 251 4390 +/- 166 -0.8133916 0.1606008 0.2256984 C3 complement component 3 2677 +/- 238 3668 +/- 587 1655 +/- 154 2039 +/- 196 -0.0037930 0.1642487 0.1618787 Pcm1 pericentriolar material 1 276 +/- 35 351 +/- 37 376 +/- 43 381 +/- 24 0.0827748 0.1671968 0.9247813 Eif5 eukaryotic translation initiation factor 5 8827 +/- 251 9331 +/- 262 7941 +/- 139 8250 +/- 152 -0.0002345 0.1949471 0.1698313 Rpl18 ribosomal protein L18 10779 +/- 411 11534 +/- 392 8934 +/- 293 9623 +/- 357 -0.0000009 0.2127203 0.1731781 Psmb8 proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctiona 94 +/- 14 121 +/- 11 98 +/- 7 120 +/- 15 0.8999676 0.1734383 0.2419583

S3- Aging related genes in rhesus and rat.xls Page 9 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Scap2 src family associated phosphoprotein 2 209 +/- 21 253 +/- 20 211 +/- 21 215 +/- 19 -0.2261262 0.1735452 0.8775638 Vcp valosin-containing protein 1102 +/- 39 1144 +/- 45 966 +/- 36 1049 +/- 44 -0.0109398 0.4980118 0.1826003 Plec1 plectin 1, intermediate filament binding protein 500kDa 3168 +/- 240 3605 +/- 190 3366 +/- 213 3724 +/- 122 0.3068389 0.1853166 0.1834081 Bhlhb3 basic helix-loop-helix domain containing, class B, 3 119 +/- 21 160 +/- 29 214 +/- 15 265 +/- 31 0.0007434 0.2717205 0.1836970 Glul glutamate-ammonia ligase (glutamine synthetase) 14106 +/- 1429 15962 +/- 1614 14216 +/- 1203 16286 +/- 811 0.8407058 0.4095551 0.1899573 Igfbp6 insulin-like growth factor binding protein 6 795 +/- 47 804 +/- 66 655 +/- 38 750 +/- 54 -0.0153002 0.9125309 0.1911073 Pdk2 pyruvate dehydrogenase kinase, isozyme 2 858 +/- 49 910 +/- 38 1065 +/- 42 1142 +/- 36 0.0002650 0.4284164 0.1925428 Mog myelin oligodendrocyte glycoprotein 4168 +/- 662 5730 +/- 1129 2440 +/- 425 3331 +/- 467 -0.0223406 0.2665493 0.1930741 Ctgf connective tissue growth factor 756 +/- 161 908 +/- 140 271 +/- 40 368 +/- 55 -0.0006944 0.4949783 0.1935612 Enpp2 ectonucleotide pyrophosphatase/phosphodiesterase 2 (autotaxin) 3043 +/- 553 3566 +/- 683 1105 +/- 243 1628 +/- 295 -0.0009880 0.5654331 0.2071009 Tgfb2 Transforming growth factor, beta 2 228 +/- 32 306 +/- 56 395 +/- 33 454 +/- 28 0.0058972 0.2570186 0.2131912 Erp29 endoplasmic reticulum protein 29 2591 +/- 270 3030 +/- 187 2475 +/- 50 2637 +/- 169 -0.2314625 0.2142378 0.4044746 Lcp2 lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 7 200 +/- 23 250 +/- 30 148 +/- 13 171 +/- 24 -0.0003787 0.2152897 0.4413804 Rps8 ribosomal protein S8 7154 +/- 320 7698 +/- 258 6230 +/- 225 6609 +/- 223 -0.0004546 0.2167886 0.2614040 Hibadh 3-hydroxyisobutyrate dehydrogenase 953 +/- 34 1018 +/- 38 924 +/- 36 1001 +/- 44 -0.4438434 0.2318732 0.2170234 Nol3 nucleolar protein 3 (apoptosis repressor with CARD domain) 438 +/- 45 519 +/- 42 423 +/- 42 465 +/- 28 -0.4094220 0.2175803 0.4258190 Unc5B unc-5 homolog B (C. elegans) 1063 +/- 172 1437 +/- 225 776 +/- 87 783 +/- 33 -0.0140969 0.2183388 0.9469340 Fnbp1 Formin binding protein 1 2180 +/- 284 2827 +/- 398 1337 +/- 59 1373 +/- 54 -0.0016667 0.2188058 0.6667091 Ptpn23 protein tyrosine phosphatase, non-receptor type 23 151 +/- 10 158 +/- 7 380 +/- 24 437 +/- 34 0.0000006 0.5989845 0.2192745 Itpr2 inositol 1,4,5-triphosphate receptor, type 2 207 +/- 24 269 +/- 41 207 +/- 22 224 +/- 12 -0.2629609 0.2217053 0.5279752 Npc2 Niemann-Pick disease, type C2 3019 +/- 294 3544 +/- 283 2140 +/- 161 2387 +/- 146 -0.0014036 0.2267512 0.2840053 Ndrg2 NDRG family member 2 18345 +/- 695 18774 +/- 863 17718 +/- 792 19181 +/- 804 -0.9317219 0.7068393 0.2277965 Nqo1 NAD(P)H dehydrogenase, quinone 1 486 +/- 70 634 +/- 93 447 +/- 48 525 +/- 80 -0.1698396 0.2350849 0.4292454 Abca2 ATP-binding cassette, sub-family A (ABC1), member 2 3508 +/- 582 4558 +/- 624 2835 +/- 141 3151 +/- 200 -0.0440307 0.2467877 0.2352910 Ddr1 discoidin domain receptor family, member 1 4423 +/- 279 4934 +/- 300 4776 +/- 104 4805 +/- 83 0.5664858 0.2405970 0.8311610 Lgals1 lectin, galactoside-binding, soluble, 1 (galectin 1) 1561 +/- 151 2182 +/- 457 1000 +/- 65 1126 +/- 127 -0.0011326 0.2440003 0.4105198 Arid4B AT rich interactive domain 4B (RBP1- like) 316 +/- 29 368 +/- 31 332 +/- 10 352 +/- 14 -0.8350039 0.2474180 0.2808336 Gpr83 G protein-coupled receptor 83 865 +/- 102 1046 +/- 106 997 +/- 133 1169 +/- 237 0.2661960 0.2474181 0.5486987 Mapk6 mitogen-activated protein kinase 6 3225 +/- 189 3472 +/- 168 2883 +/- 80 3049 +/- 106 -0.0240340 0.3531164 0.2475687 Tmod1 tropomodulin 1 417 +/- 23 439 +/- 35 462 +/- 34 520 +/- 32 0.1627506 0.6028933 0.2501989 Gnb1 Guanine nucleotide binding protein (G protein), beta polypeptide 1 353 +/- 46 428 +/- 41 522 +/- 40 546 +/- 40 0.0003344 0.2541550 0.6763455 Eef2 eukaryotic translation elongation factor 2 4388 +/- 465 5007 +/- 176 4613 +/- 307 5049 +/- 258 0.5367915 0.2569351 0.3060227 Cutl1 Cut-like 1, CCAAT displacement protein (Drosophila) 378 +/- 77 485 +/- 43 522 +/- 91 525 +/- 41 0.0062172 0.2576020 0.9794509 Rps19 ribosomal protein S19 13041 +/- 438 13747 +/- 408 10634 +/- 404 11010 +/- 220 -0.0000017 0.2654267 0.4381949 Cebpg CCAAT/enhancer binding protein (C/EBP), gamma 1085 +/- 18 1141 +/- 43 915 +/- 21 949 +/- 30 -0.0000001 0.2677950 0.3806164 Mcl1 myeloid cell leukemia sequence 1 (BCL2-related) 228 +/- 27 285 +/- 41 242 +/- 30 244 +/- 13 -0.6128966 0.2708576 0.9427433 Slc6A8 solute carrier family 6 (neurotransmitter transporter, creatine), member 8 1035 +/- 135 1096 +/- 125 1491 +/- 82 1629 +/- 86 0.0020302 0.7458516 0.2740694 Pdk4 pyruvate dehydrogenase kinase, isozyme 4 187 +/- 26 201 +/- 44 179 +/- 20 210 +/- 18 0.6028056 0.7887622 0.2747174 Srebf1 sterol regulatory element binding transcription factor 1 2104 +/- 259 2329 +/- 174 1764 +/- 82 1893 +/- 76 -0.0362036 0.4898340 0.2795539 Pygl phosphorylase, glycogen; liver (Hers disease, glycogen storage disease type 160 +/- 29 223 +/- 46 112 +/- 15 126 +/- 25 -0.0050562 0.2807569 0.6481656 Rpl4 ribosomal protein L4 384 +/- 14 409 +/- 17 407 +/- 22 409 +/- 25 0.5636868 0.2926158 0.9469728 Apoe apolipoprotein E 4911 +/- 575 5291 +/- 415 4507 +/- 729 5576 +/- 645 -0.6985110 0.6051368 0.3004621 Rims1 regulating synaptic membrane exocytosis 1 157 +/- 25 198 +/- 28 204 +/- 31 228 +/- 25 0.0735058 0.3020276 0.5682607 Cnn3 calponin 3, acidic 3895 +/- 265 4259 +/- 217 4114 +/- 215 4335 +/- 181 0.6282432 0.3145390 0.4503497 Bcl2L1 BCL2-like 1 216 +/- 37 263 +/- 25 181 +/- 30 209 +/- 31 -0.1759850 0.3297557 0.5249078 Cd37 CD37 molecule 340 +/- 29 391 +/- 47 266 +/- 7 287 +/- 28 -0.0165252 0.3776994 0.4840415 Nme6 non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) 323 +/- 15 336 +/- 10 337 +/- 15 358 +/- 18 0.1932614 0.4850175 0.4072801 Myo5A myosin VA (heavy polypeptide 12, myoxin) 9504 +/- 824 10302 +/- 557 8508 +/- 469 8961 +/- 216 -0.0077248 0.4437191 0.4094266 Tnfrsf1A tumor necrosis factor receptor superfamily, member 1A 690 +/- 39 724 +/- 15 639 +/- 33 674 +/- 26 -0.0320372 0.4472086 0.4103849 Cib1 calcium and integrin binding 1 (calmyrin) 763 +/- 61 776 +/- 44 577 +/- 33 613 +/- 27 -0.0019397 0.8647032 0.4215863 Decr1 2,4-dienoyl CoA reductase 1, mitochondrial 510 +/- 33 562 +/- 54 560 +/- 32 619 +/- 67 0.1439405 0.4281085 0.4590942 Jtb jumping translocation breakpoint 5670 +/- 182 5899 +/- 212 5330 +/- 174 5451 +/- 225 -0.0001632 0.4332970 0.6828229 Acads acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain 311 +/- 31 346 +/- 34 261 +/- 15 265 +/- 24 -0.0218185 0.4642014 0.8768870 Txnip thioredoxin interacting protein 5289 +/- 536 5717 +/- 204 3799 +/- 467 4165 +/- 165 -0.0000227 0.4813546 0.4862027 Timp2 TIMP metallopeptidase inhibitor 2 359 +/- 72 394 +/- 67 451 +/- 68 517 +/- 77 0.2483089 0.7365934 0.5398178 Ralgds ral guanine nucleotide dissociation stimulator 1650 +/- 149 1792 +/- 196 970 +/- 61 1034 +/- 81 -0.0001141 0.5789536 0.5462520 Hapln2 hyaluronan and proteoglycan link protein 2 1664 +/- 368 1772 +/- 329 879 +/- 126 1005 +/- 169 -0.0107892 0.8324099 0.5654092 Slco3A1 solute carrier organic anion transporter family, member 3A1 1006 +/- 122 1086 +/- 66 881 +/- 69 914 +/- 56 -0.0358357 0.5790633 0.7219657 Cbfb core-binding factor, beta subunit 154 +/- 24 172 +/- 20 143 +/- 8 154 +/- 35 -0.5351414 0.5820813 0.7783966 Picalm phosphatidylinositol binding clathrin assembly protein 1015 +/- 125 1129 +/- 156 1035 +/- 115 1098 +/- 94 -0.7648469 0.5824581 0.6835197

S3- Aging related genes in rhesus and rat.xls Page 10 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Ifitm3 interferon induced transmembrane protein 3 (1-8U) 119 +/- 10 128 +/- 25 116 +/- 8 127 +/- 17 -0.4017021 0.7627126 0.5886238 Aif1 allograft inflammatory factor 1 304 +/- 36 332 +/- 38 246 +/- 22 256 +/- 20 -0.0322170 0.6062468 0.7402166 Padi2 peptidyl arginine deiminase, type II 143 +/- 36 169 +/- 46 201 +/- 24 202 +/- 38 0.3076244 0.6628414 0.9901754 Cspg5 chondroitin sulfate proteoglycan 5 (neuroglycan C) 645 +/- 130 684 +/- 71 783 +/- 137 842 +/- 34 0.0433197 0.7995659 0.6894908 Anxa3 annexin A3 179 +/- 17 187 +/- 12 172 +/- 13 182 +/- 25 -0.6010575 0.7090863 0.7229061 Gnai2 guanine nucleotide binding protein (G protein), alpha inhibiting activity polype 1209 +/- 75 1245 +/- 68 1205 +/- 42 1240 +/- 80 -0.7659332 0.7239160 0.7134297 Dll1 delta-like 1 (Drosophila) 166 +/- 14 173 +/- 22 203 +/- 21 212 +/- 15 0.1269054 0.7917236 0.7487577 Adamts1 ADAM metallopeptidase with thrombospondin type 1 motif, 1 128 +/- 24 137 +/- 31 104 +/- 14 105 +/- 24 -0.1352877 0.8083947 0.9766611 Fxyd1 FXYD domain containing ion transport regulator 1 (phospholemman) 4254 +/- 282 4317 +/- 263 4481 +/- 302 4511 +/- 256 0.2722129 0.8731544 0.9418538 Stxbp2 syntaxin binding protein 2 135 +/- 12 137 +/- 13 118 +/- 9 119 +/- 12 -0.0961304 0.9066216 0.9766021 Down in Rat Aging Studies Only Slc17A6 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporte 902 +/- 242 895 +/- 168 1208 +/- 91 1179 +/- 134 0.1764563 -0.9835658 -0.8599302 Btg2 BTG family, member 2 122 +/- 14 118 +/- 16 140 +/- 13 135 +/- 10 0.1399547 -0.8669667 -0.7861833 Adcy5 adenylate cyclase 5 782 +/- 43 764 +/- 28 636 +/- 14 627 +/- 29 -0.0001256 -0.7277576 -0.7950049 Col11A1 Collagen, type XI, alpha 1 242 +/- 36 233 +/- 53 334 +/- 77 299 +/- 25 0.0083434 -0.8907137 -0.6830708 Madd MAP-kinase activating death domain 2078 +/- 156 1974 +/- 159 2452 +/- 135 2445 +/- 162 0.0000992 -0.6518347 -0.9712544 Camk2D calcium/calmodulin-dependent protein kinase (CaM kinase) II delta 7913 +/- 785 7412 +/- 778 3163 +/- 483 2842 +/- 481 -0.0000020 -0.6594735 -0.6491775 Adrbk1 adrenergic, beta, receptor kinase 1 285 +/- 27 268 +/- 21 265 +/- 30 256 +/- 42 -0.6075008 -0.6300619 -0.8680339 Ca4 carbonic anhydrase IV 614 +/- 77 577 +/- 69 979 +/- 67 927 +/- 74 0.0003622 -0.7276679 -0.6169736 Csrp2 cysteine and glycine-rich protein 2 211 +/- 30 185 +/- 24 159 +/- 19 145 +/- 11 -0.0336570 -0.5319547 -0.5538771 Ppm1G protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isofo 878 +/- 33 852 +/- 20 906 +/- 20 891 +/- 11 0.0226414 -0.5198417 -0.5370966 Ppm2C protein phosphatase 2C, magnesium-dependent, catalytic subunit 3113 +/- 189 2927 +/- 234 2699 +/- 186 2556 +/- 48 -0.0874371 -0.5491130 -0.4882572 Igfbp2 insulin-like growth factor binding protein 2, 36kDa 370 +/- 44 326 +/- 37 652 +/- 87 579 +/- 101 0.0008725 -0.4851884 -0.5982331 Ppfia4 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacti 1441 +/- 132 1292 +/- 155 2032 +/- 145 1947 +/- 101 0.0003794 -0.4842730 -0.6450353 Kcnf1 potassium voltage-gated channel, subfamily F, member 1 1301 +/- 149 1157 +/- 130 914 +/- 59 867 +/- 82 -0.0046032 -0.4823173 -0.6512262 Myh6 myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypert 278 +/- 33 245 +/- 30 327 +/- 31 321 +/- 22 0.0660676 -0.4771881 -0.8794734 Cacnb2 calcium channel, voltage-dependent, beta 2 subunit 715 +/- 48 653 +/- 54 799 +/- 59 786 +/- 48 0.0299949 -0.4160334 -0.8665600 Hspa4 Heat shock 70kDa protein 4 3762 +/- 367 3378 +/- 245 3542 +/- 138 3432 +/- 190 -0.5041630 -0.4072292 -0.6521646 Prkar1A protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific ex 3090 +/- 443 2621 +/- 285 2802 +/- 285 2785 +/- 336 -0.5545442 -0.3975163 -0.9706189 Dpysl4 dihydropyrimidinase-like 4 1199 +/- 32 1102 +/- 101 1215 +/- 55 1206 +/- 80 0.1187996 -0.3963646 -0.9275164 Kit v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog 4667 +/- 898 3619 +/- 752 5985 +/- 275 5401 +/- 655 0.0100688 -0.3927923 -0.4458483 Pou3F1 POU domain, class 3, transcription factor 1 5136 +/- 1055 4008 +/- 595 1167 +/- 451 1031 +/- 340 -0.0008464 -0.3792670 -0.8157391 Rgs14 regulator of G-protein signalling 14 298 +/- 89 208 +/- 22 207 +/- 5 204 +/- 13 -0.3689393 -0.3672639 -0.8628189 Tmsb10 thymosin, beta 10 19055 +/- 1135 17143 +/- 1661 14060 +/- 1235 13713 +/- 1031 -0.0004159 -0.3671934 -0.8343127 Cacna1B calcium channel, voltage-dependent, L type, alpha 1B subunit 1982 +/- 101 1808 +/- 172 2242 +/- 49 2084 +/- 149 0.0298995 -0.4076252 -0.3622133 Nxf1 nuclear RNA export factor 1 3265 +/- 226 3157 +/- 115 3275 +/- 143 3118 +/- 75 -0.8600065 -0.6841242 -0.3605383 Gprasp1 G protein-coupled receptor associated sorting protein 1 4611 +/- 221 4291 +/- 243 3914 +/- 135 3833 +/- 188 -0.0061492 -0.3533829 -0.7365751 Eif4E eukaryotic translation initiation factor 4E 3169 +/- 170 2943 +/- 156 2890 +/- 146 2731 +/- 85 -0.1546955 -0.3497306 -0.3739587 Adcy8 adenylate cyclase 8 (brain) 842 +/- 32 772 +/- 62 492 +/- 39 485 +/- 16 -0.0000127 -0.3462817 -0.8748631 Hdgf hepatoma-derived growth factor (high-mobility group protein 1-like) 2905 +/- 89 2801 +/- 95 2676 +/- 61 2604 +/- 36 -0.0440635 -0.4434101 -0.3409014 Wrnip1 Werner helicase interacting protein 1 2354 +/- 101 2186 +/- 134 3033 +/- 152 2971 +/- 26 0.0000426 -0.3406765 -0.6986370 Neu1 sialidase 1 (lysosomal sialidase) 678 +/- 30 616 +/- 53 647 +/- 52 590 +/- 48 -0.1012240 -0.3384211 -0.4384886 Gabrd gamma-aminobutyric acid (GABA) A receptor, delta 701 +/- 68 649 +/- 83 1003 +/- 85 887 +/- 75 0.0269858 -0.6363141 -0.3336106 Rab4B RAB4B, member RAS oncogene family 2461 +/- 101 2309 +/- 109 2161 +/- 63 2153 +/- 84 -0.0665174 -0.3305738 -0.9368148 Olfm3 olfactomedin 3 567 +/- 99 458 +/- 41 221 +/- 45 166 +/- 25 -0.0001225 -0.3433617 -0.3197715 Ctbp1 C-terminal binding protein 1 2888 +/- 204 2604 +/- 172 3515 +/- 93 3484 +/- 319 0.0000783 -0.3112263 -0.9302968 Flot2 flotillin 2 1471 +/- 66 1362 +/- 76 1778 +/- 32 1705 +/- 72 0.0000211 -0.3066409 -0.3914526 Myo5B myosin VB 327 +/- 86 237 +/- 59 476 +/- 56 404 +/- 35 0.0231608 -0.4061175 -0.3063663 Kcnip4 Kv channel interacting protein 4 1579 +/- 121 1440 +/- 183 1673 +/- 153 1407 +/- 182 0.9504456 -0.5431295 -0.2952709 Scrg1 scrapie responsive protein 1 2376 +/- 217 2095 +/- 125 1932 +/- 160 1732 +/- 278 -0.0446596 -0.2951793 -0.5548998 Gnas GNAS complex locus 3449 +/- 199 3168 +/- 250 4315 +/- 161 3991 +/- 231 0.0000165 -0.4015160 -0.2849155 Ttr transthyretin (prealbumin, amyloidosis type I) 435 +/- 177 190 +/- 60 458 +/- 177 269 +/- 112 0.4852815 -0.2844008 -0.3918960 Nr4A2 nuclear receptor subfamily 4, group A, member 2 769 +/- 158 560 +/- 84 472 +/- 79 451 +/- 48 -0.0470992 -0.2784260 -0.8263460 Ppp2R2C protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), gamma is 1920 +/- 87 1787 +/- 75 1505 +/- 116 1415 +/- 120 -0.0003213 -0.2752064 -0.6049540 Dio2 deiodinase, iodothyronine, type II 167 +/- 41 112 +/- 21 144 +/- 10 139 +/- 15 0.8484750 -0.2702075 -0.7862242 Impa1 inositol(myo)-1(or 4)-monophosphatase 1 1283 +/- 103 1184 +/- 46 1257 +/- 31 1171 +/- 63 -0.6882145 -0.4122801 -0.2658341 Synpr synaptoporin 7801 +/- 1027 6762 +/- 1198 12700 +/- 298 11939 +/- 542 0.0000549 -0.5256297 -0.2624811 Actn1 actinin, alpha 1 3839 +/- 279 3397 +/- 240 4211 +/- 216 4122 +/- 276 0.0325171 -0.2588756 -0.8047270 Aacs acetoacetyl-CoA synthetase 1751 +/- 102 1591 +/- 86 1523 +/- 75 1481 +/- 49 -0.0162333 -0.2587777 -0.6488343 Tubb3 tubulin, beta 3 2758 +/- 164 2409 +/- 237 2608 +/- 109 2390 +/- 172 -0.4130377 -0.2577232 -0.3201985

S3- Aging related genes in rhesus and rat.xls Page 11 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Arl1 ADP-ribosylation factor-like 1 1349 +/- 146 1142 +/- 86 1453 +/- 83 1392 +/- 83 0.1329023 -0.2575022 -0.6179959 Jun v-jun sarcoma virus 17 oncogene homolog (avian) 700 +/- 55 634 +/- 49 1304 +/- 111 1155 +/- 39 0.0000594 -0.3917051 -0.2506023 Syt4 synaptotagmin IV 9719 +/- 546 8818 +/- 490 10923 +/- 459 10364 +/- 486 0.0046699 -0.2473968 -0.4249406 Ap3M2 Adaptor-related protein complex 3, mu 2 subunit 112 +/- 11 103 +/- 9 132 +/- 10 114 +/- 11 0.1997690 -0.5198280 -0.2427328 Grin1 glutamate receptor, ionotropic, N-methyl D-aspartate 1 3870 +/- 384 3282 +/- 267 4398 +/- 321 3934 +/- 220 0.0025778 -0.2399177 -0.2644958 Rabggta Rab geranylgeranyltransferase, alpha subunit 889 +/- 42 857 +/- 33 884 +/- 23 832 +/- 32 -0.3489373 -0.5593272 -0.2178008 Prkcb1 Protein kinase C, beta 1 2174 +/- 618 1285 +/- 104 803 +/- 83 774 +/- 98 -0.0163718 -0.2119266 -0.8247185 Sumo2 SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) 12943 +/- 496 11884 +/- 615 16502 +/- 609 16284 +/- 549 0.0000299 -0.2112664 -0.7961450 Rgs7 regulator of G-protein signalling 7 794 +/- 71 668 +/- 60 873 +/- 57 791 +/- 27 0.1398040 -0.2035436 -0.2346507 Pctk1 PCTAIRE protein kinase 1 623 +/- 24 568 +/- 32 793 +/- 32 758 +/- 34 0.0000250 -0.1954851 -0.4715217 Lum lumican 437 +/- 84 293 +/- 58 385 +/- 107 363 +/- 44 0.8125121 -0.1935834 -0.8558000 Ndufv2 NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa 3532 +/- 76 3362 +/- 94 3553 +/- 80 3385 +/- 134 0.9152323 -0.1916097 -0.3198443 Kcnmb4 potassium large conductance calcium-activated channel, subfamily M, beta m 4385 +/- 214 3933 +/- 241 3832 +/- 305 3761 +/- 273 -0.1854436 -0.1912233 -0.8661861 Spon1 spondin 1, extracellular matrix protein 3932 +/- 216 3444 +/- 271 2878 +/- 136 2785 +/- 177 -0.0004018 -0.1908226 -0.6891389 Prkar2B protein kinase, cAMP-dependent, regulatory, type II, beta 3106 +/- 228 2727 +/- 129 2921 +/- 117 2710 +/- 127 -0.1774722 -0.1856242 -0.2551475 Stk39 serine threonine kinase 39 (STE20/SPS1 homolog, yeast) 1847 +/- 187 1846 +/- 170 1220 +/- 71 1109 +/- 21 -0.0002523 -0.9992789 -0.1840012 Cntnap1 contactin associated protein 1 3407 +/- 281 3364 +/- 250 3245 +/- 211 2852 +/- 171 -0.1115418 -0.9116809 -0.1815519 Smpd3 sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomye 1229 +/- 172 973 +/- 155 1527 +/- 73 1365 +/- 83 0.0041741 -0.2945442 -0.1788589 Dnm1L dynamin 1-like 1354 +/- 93 1195 +/- 43 1395 +/- 78 1327 +/- 68 0.1464051 -0.1650452 -0.5281813 Atp2A2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 6511 +/- 315 5809 +/- 343 6375 +/- 208 6016 +/- 283 0.6808326 -0.1625754 -0.3379554 B4Galt6 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6 3702 +/- 250 3117 +/- 295 4186 +/- 123 4025 +/- 189 0.0020351 -0.1612396 -0.4982988 Irs2 insulin receptor substrate 2 5710 +/- 303 5168 +/- 167 4725 +/- 171 4676 +/- 173 -0.0110467 -0.1563534 -0.8439038 Maged2 melanoma antigen family D, 2 161 +/- 23 129 +/- 8 157 +/- 25 114 +/- 10 -0.6241616 -0.2262033 -0.1555367 Sh3Kbp1 SH3-domain kinase binding protein 1 986 +/- 71 824 +/- 87 753 +/- 42 615 +/- 74 -0.0069100 -0.1819558 -0.1535237 Hfe hemochromatosis 171 +/- 34 112 +/- 14 205 +/- 34 169 +/- 21 0.0139458 -0.1497135 -0.3845483 Calu calumenin 1229 +/- 91 1066 +/- 40 1331 +/- 57 1230 +/- 23 0.0103953 -0.1466325 -0.1459970 Lxn latexin 599 +/- 97 409 +/- 66 350 +/- 98 211 +/- 10 -0.0127412 -0.1414969 -0.2164585 Drb1 developmentally regulated RNA-binding protein 1 747 +/- 41 661 +/- 33 647 +/- 19 614 +/- 19 -0.0111814 -0.1383381 -0.2553773 Sip1 survival of motor neuron protein interacting protein 1 279 +/- 20 215 +/- 33 293 +/- 33 255 +/- 15 0.5654550 -0.1364447 -0.3295476 Mmp16 matrix metallopeptidase 16 (membrane-inserted) 267 +/- 38 205 +/- 20 387 +/- 31 308 +/- 36 0.0005269 -0.1916111 -0.1358128 Tnc Tenascin C (hexabrachion) 123 +/- 12 95 +/- 12 170 +/- 14 135 +/- 16 0.0056985 -0.1348806 -0.1368609 Sstr1 somatostatin receptor 1 707 +/- 107 496 +/- 68 698 +/- 72 588 +/- 72 0.6642807 -0.1342815 -0.3076193 Dscam Down syndrome cell adhesion molecule 513 +/- 35 438 +/- 30 494 +/- 29 472 +/- 28 0.7994537 -0.1335554 -0.5953409 Mtpn myotrophin 9221 +/- 411 8161 +/- 473 9817 +/- 285 9520 +/- 335 0.0177722 -0.1223914 -0.5176720 Cacna1D calcium channel, voltage-dependent, L type, alpha 1D subunit 275 +/- 14 241 +/- 31 393 +/- 22 346 +/- 16 0.0004820 -0.3451331 -0.1207091 Atxn10 ataxin 10 7387 +/- 309 6658 +/- 277 7155 +/- 165 6860 +/- 168 -0.9533112 -0.1104730 -0.2435040 Mpzl1 myelin protein zero-like 1 127 +/- 19 119 +/- 8 157 +/- 17 122 +/- 9 0.3689070 -0.7005603 -0.1100686 Ppp3Cb protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineu15152 +/- 1195 12631 +/- 714 12302 +/- 318 11683 +/- 323 -0.0386873 -0.1069854 -0.2056822 Syt5 synaptotagmin V 1590 +/- 119 1315 +/- 96 2095 +/- 145 1945 +/- 55 0.0000329 -0.1048000 -0.3679430 Coro1A coronin, actin binding protein, 1A 1257 +/- 114 1020 +/- 57 834 +/- 46 816 +/- 54 -0.0046753 -0.1027082 -0.8012305 Egr1 early growth response 1 233 +/- 29 173 +/- 15 205 +/- 13 168 +/- 15 -0.2665383 -0.1072100 -0.1011808 Safb scaffold attachment factor B 1532 +/- 134 1300 +/- 132 1486 +/- 73 1283 +/- 80 -0.2585838 -0.2445264 -0.0954789 Gpd2 glycerol-3-phosphate dehydrogenase 2 (mitochondrial) 2261 +/- 127 1979 +/- 148 2578 +/- 83 2297 +/- 121 0.0108904 -0.1789762 -0.0953327 Rab2 RAB2, member RAS oncogene family 867 +/- 89 668 +/- 49 784 +/- 73 643 +/- 71 -0.1457303 -0.0885972 -0.2017205 Scn2B sodium channel, voltage-gated, type II, beta 5113 +/- 69 4612 +/- 234 4913 +/- 202 4910 +/- 177 0.9080094 -0.0871552 -0.9904250 Syt7 synaptotagmin VII 114 +/- 17 89 +/- 14 257 +/- 20 201 +/- 21 0.0000281 -0.2873830 -0.0865606 Tacr1 tachykinin receptor 1 118 +/- 3 103 +/- 12 193 +/- 23 142 +/- 11 0.0023689 -0.2599580 -0.0856042 Bckdhb branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup 919 +/- 59 784 +/- 36 912 +/- 32 883 +/- 55 0.3338507 -0.0842831 -0.6679067 Tcp1 t-complex 1 3494 +/- 291 2853 +/- 122 3105 +/- 55 2928 +/- 107 -0.4544495 -0.0837687 -0.1900724 Psma1 proteasome (prosome, macropain) subunit, alpha type, 1 2881 +/- 149 2517 +/- 112 3221 +/- 146 2822 +/- 191 0.0494849 -0.0819812 -0.1355033 Dcamkl1 Doublecortin and CaM kinase-like 1 6154 +/- 315 5802 +/- 319 6877 +/- 374 5921 +/- 307 0.1320771 -0.4504813 -0.0795961 Bad BCL2-antagonist of cell death 209 +/- 15 173 +/- 10 175 +/- 14 142 +/- 18 -0.0477613 -0.0792615 -0.1911432 Nell1 NEL-like 1 (chicken) 2297 +/- 225 1832 +/- 209 3169 +/- 220 2599 +/- 185 0.0000152 -0.1603789 -0.0787580 Gnaz guanine nucleotide binding protein (G protein), alpha z polypeptide 1857 +/- 101 1699 +/- 201 1760 +/- 110 1480 +/- 88 -0.3190603 -0.5036353 -0.0777622 Kcnv1 potassium channel, subfamily V, member 1 194 +/- 32 123 +/- 8 173 +/- 25 145 +/- 18 0.9129403 -0.0768855 -0.3933686 Npy neuropeptide Y 2431 +/- 393 2354 +/- 346 2387 +/- 348 1610 +/- 96 -0.1608260 -0.8851595 -0.0767649 Nudc nuclear distribution gene C homolog (A. nidulans) 416 +/- 31 398 +/- 27 405 +/- 31 328 +/- 20 -0.1185474 -0.6608070 -0.0736951 Camk2A calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha 1142 +/- 306 753 +/- 139 2093 +/- 147 1714 +/- 114 0.0027982 -0.2858937 -0.0726365 Nr4A3 nuclear receptor subfamily 4, group A, member 3 1621 +/- 76 1285 +/- 140 1691 +/- 80 1403 +/- 136 0.1507312 -0.0695149 -0.1127868 Pacs1 phosphofurin acidic cluster sorting protein 1 1143 +/- 107 979 +/- 113 1099 +/- 53 969 +/- 31 -0.9045245 -0.3178664 -0.0687470

S3- Aging related genes in rhesus and rat.xls Page 12 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Ptpro protein tyrosine phosphatase, receptor type, O 182 +/- 28 154 +/- 7 190 +/- 7 136 +/- 22 -0.9613774 -0.3692253 -0.0680233 Robo1 Roundabout, axon guidance receptor, homolog 1 (Drosophila) 342 +/- 33 264 +/- 31 489 +/- 6 390 +/- 40 0.0003866 -0.1103317 -0.0663500 Dusp6 dual specificity phosphatase 6 2476 +/- 370 1560 +/- 213 892 +/- 95 753 +/- 79 -0.0013380 -0.0640929 -0.2901094 Fkbp4 FK506 binding protein 4, 59kDa 2670 +/- 102 2376 +/- 106 2656 +/- 93 2428 +/- 45 0.7423512 -0.0730113 -0.0621010 Slc7A10 solute carrier family 7, (neutral amino acid transporter, y+ system) member 1 357 +/- 22 330 +/- 22 416 +/- 20 346 +/- 25 0.0389038 -0.3933736 -0.0605756 Scamp1 Secretory carrier membrane protein 1 2137 +/- 86 1819 +/- 119 2099 +/- 116 1898 +/- 94 0.8043134 -0.0579743 -0.2100475 Mark1 MAP/microtubule affinity-regulating kinase 1 803 +/- 91 576 +/- 38 699 +/- 67 547 +/- 62 -0.1761843 -0.0571661 -0.1322871 Hmgcr 3-hydroxy-3-methylglutaryl-Coenzyme A reductase 1890 +/- 172 1539 +/- 69 2130 +/- 95 1817 +/- 102 0.0473670 -0.1019236 -0.0526806 Ube2G1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 643 +/- 51 771 +/- 63 502 +/- 12 695 +/- 17 -0.0213582 0.1500320 0.0000234 Vdp vesicle docking protein p115 333 +/- 23 487 +/- 24 387 +/- 27 440 +/- 22 0.4782452 0.0008437 0.1629648 Pld2 phospholipase D2 201 +/- 20 312 +/- 13 231 +/- 27 280 +/- 18 -0.8057218 0.0013884 0.1656428 Fez1 fasciculation and elongation protein zeta 1 (zygin I) 8114 +/- 604 9755 +/- 837 6969 +/- 218 8397 +/- 232 -0.0323666 0.1460565 0.0016351 Adprh ADP-ribosylarginine hydrolase 189 +/- 23 309 +/- 20 168 +/- 16 281 +/- 20 -0.0153821 0.0030101 0.0023404 Eno1 enolase 1, (alpha) 7846 +/- 333 9198 +/- 336 6480 +/- 135 7880 +/- 253 -0.0003912 0.0169672 0.0025195 Bpnt1 3'(2'), 5'-bisphosphate nucleotidase 1 161 +/- 18 269 +/- 23 210 +/- 23 267 +/- 28 0.1584836 0.0048843 0.1565358 Slc15A4 Solute carrier family 15, member 4 630 +/- 46 885 +/- 55 671 +/- 24 756 +/- 14 -0.4346732 0.0053372 0.0149817 Polr2G polymerase (RNA) II (DNA directed) polypeptide G 1501 +/- 46 1883 +/- 158 1045 +/- 54 1288 +/- 36 -0.0003485 0.0603422 0.0053670 Pak2 p21 (CDKN1A)-activated kinase 2 335 +/- 25 481 +/- 33 362 +/- 10 410 +/- 22 -0.4680143 0.0059192 0.0965466 Gamt guanidinoacetate N-methyltransferase 1022 +/- 43 1199 +/- 29 1147 +/- 61 1359 +/- 156 0.0431660 0.0094010 0.2610730 Sc4Mol sterol-C4-methyl oxidase-like 5593 +/- 587 6927 +/- 418 5526 +/- 348 7175 +/- 378 0.8695356 0.0972138 0.0112460 Nme1 Non-metastatic cells 1, protein (NM23A) expressed in 1207 +/- 74 1482 +/- 34 1218 +/- 92 1399 +/- 47 -0.4883093 0.0116430 0.1198986 Slc14A1 Solute carrier family 14 (urea transporter), member 1 (Kidd blood group) 669 +/- 53 1000 +/- 92 657 +/- 68 883 +/- 90 -0.4189439 0.0141667 0.0795725 Psmb7 Proteasome (prosome, macropain) subunit, beta type, 7 371 +/- 27 515 +/- 39 467 +/- 43 650 +/- 85 0.0079241 0.0143763 0.1031129 Tpm1 Tropomyosin 1 (alpha) 396 +/- 71 679 +/- 70 626 +/- 100 856 +/- 98 0.0007556 0.0180423 0.1358359 Nr2F1 Nuclear receptor subfamily 2, group F, member 1 3880 +/- 234 4227 +/- 278 4775 +/- 257 5717 +/- 210 0.0012320 0.3628789 0.0197335 Rab12 RAB12, member RAS oncogene family 321 +/- 25 439 +/- 34 442 +/- 78 498 +/- 58 0.0349318 0.0211394 0.5815494 Ppm1B protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform 2968 +/- 253 3629 +/- 258 2741 +/- 80 2996 +/- 47 -0.0466994 0.0972619 0.0254303 Gas7 growth arrest-specific 7 9122 +/- 492 10812 +/- 463 7341 +/- 461 8180 +/- 533 -0.0001930 0.0313393 0.2659777 Itgb1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 include 657 +/- 64 790 +/- 95 641 +/- 35 744 +/- 25 -0.3751664 0.2811003 0.0411422 Slc37A4 solute carrier family 37 (glycerol-6-phosphate transporter), member 4 195 +/- 20 266 +/- 23 170 +/- 18 236 +/- 25 -0.0929626 0.0446113 0.0680649 Nef3 neurofilament 3 (150kDa medium) 3259 +/- 240 3569 +/- 242 3410 +/- 281 4209 +/- 212 0.1287468 0.3830351 0.0501556 Sepw1 selenoprotein W, 1 585 +/- 50 738 +/- 47 595 +/- 42 690 +/- 37 -0.7112477 0.0506265 0.1253401 Hbld2 HESB like domain containing 2 5203 +/- 248 5912 +/- 354 5130 +/- 262 6496 +/- 509 0.1457051 0.1351919 0.0540074 Sh3Bp4 SH3-domain binding protein 4 814 +/- 96 1038 +/- 89 566 +/- 21 669 +/- 39 -0.0009859 0.1186400 0.0564387 Elovl6 ELOVL family member 6, elongation of long chain fatty acids (FEN1/Elo2, SU 896 +/- 196 1262 +/- 163 882 +/- 155 1274 +/- 98 -0.4382912 0.1833837 0.0640144 Rhoj ras homolog gene family, member J 171 +/- 12 208 +/- 13 156 +/- 11 198 +/- 20 -0.0888798 0.0693867 0.1148322 Rfng radical fringe homolog (Drosophila) 490 +/- 18 545 +/- 21 583 +/- 19 626 +/- 14 0.0001991 0.0700264 0.1095614 Snx16 sorting nexin 16 190 +/- 19 284 +/- 41 202 +/- 16 253 +/- 45 -0.8547074 0.0750360 0.3336320 Rgc32 response gene to complement 32 6859 +/- 127 8305 +/- 653 5556 +/- 183 6802 +/- 669 -0.0002985 0.0779701 0.1372903 Acox1 acyl-Coenzyme A oxidase 1, palmitoyl 1306 +/- 106 1447 +/- 58 1243 +/- 59 1393 +/- 50 -0.1515180 0.2791040 0.0829987 Hadha hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/e 1139 +/- 62 1306 +/- 61 887 +/- 37 962 +/- 44 -0.0003546 0.0832165 0.2268701 Fgfr1 fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syn 853 +/- 44 977 +/- 70 915 +/- 29 1117 +/- 91 0.0866015 0.1705280 0.0912459 Agpat4 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltr 616 +/- 22 811 +/- 94 644 +/- 36 696 +/- 57 -0.2965638 0.0931535 0.4606310 Nfasc neurofascin homolog (chicken) 243 +/- 22 331 +/- 47 283 +/- 36 357 +/- 13 0.5310021 0.1326910 0.1024117 Lpl lipoprotein lipase 464 +/- 38 692 +/- 112 1711 +/- 182 1760 +/- 102 0.0000017 0.1031246 0.8195307 Npepps aminopeptidase puromycin sensitive 2463 +/- 200 2784 +/- 133 2444 +/- 84 2716 +/- 120 -0.8204139 0.2157397 0.1032154 Ptpra Protein tyrosine phosphatase, receptor type, A 220 +/- 35 297 +/- 25 242 +/- 29 292 +/- 22 0.5180175 0.1062726 0.2034382 Epn2 epsin 2 1356 +/- 42 1515 +/- 77 1289 +/- 42 1368 +/- 29 -0.1358640 0.1071461 0.1617644 Ptprd Protein tyrosine phosphatase, receptor type, D 3412 +/- 455 4494 +/- 451 2387 +/- 204 2839 +/- 148 -0.0006036 0.1221485 0.1076744 Idi1 Isopentenyl-diphosphate delta isomerase 1 482 +/- 34 583 +/- 47 553 +/- 44 604 +/- 44 0.0981726 0.1174262 0.4330849 Itgae Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alp 525 +/- 30 582 +/- 15 548 +/- 16 556 +/- 35 -0.9820220 0.1239934 0.8355691 Mir16 membrane interacting protein of RGS16 117 +/- 15 140 +/- 23 72 +/- 9 98 +/- 13 -0.0266885 0.4205332 0.1389118 Dad1 defender against cell death 1 3547 +/- 95 3727 +/- 84 2771 +/- 49 2933 +/- 86 -0.0000027 0.1873880 0.1475904 Cacng2 calcium channel, voltage-dependent, gamma subunit 2 437 +/- 87 573 +/- 89 862 +/- 54 1025 +/- 88 0.0000341 0.3000925 0.1581798 Gucy1A3 Guanylate cyclase 1, soluble, alpha 3 1920 +/- 323 2521 +/- 220 2846 +/- 165 3163 +/- 421 0.0130197 0.1585896 0.5125459 Slit3 slit homolog 3 (Drosophila) 168 +/- 14 215 +/- 28 281 +/- 24 327 +/- 30 0.0015119 0.1668762 0.2641627 Gls2 glutaminase 2 (liver, mitochondrial) 82 +/- 12 93 +/- 9 102 +/- 9 138 +/- 21 0.0307679 0.4757278 0.1799443 Pdcd8 programmed cell death 8 (apoptosis-inducing factor) 568 +/- 20 580 +/- 30 482 +/- 21 531 +/- 26 -0.0327650 0.7374145 0.1848819 Per3 Period homolog 3 (Drosophila) 83 +/- 10 104 +/- 11 142 +/- 17 152 +/- 16 0.0057826 0.1891970 0.6897589 Slc7A3 solute carrier family 7 (cationic amino acid transporter, y+ system), member 224 +/- 20 260 +/- 16 268 +/- 16 274 +/- 24 0.0251458 0.2035323 0.8592245

S3- Aging related genes in rhesus and rat.xls Page 13 Young vs Aged Symbol Description Young CA Aged CA Young DG Aged DG CA v DG in CA in DG Dhcr7 7-dehydrocholesterol reductase 440 +/- 49 518 +/- 33 575 +/- 44 595 +/- 31 0.0115535 0.2184644 0.7135665 Akr1A1 aldo-keto reductase family 1, member A1 (aldehyde reductase) 1965 +/- 241 2348 +/- 190 1513 +/- 61 1657 +/- 96 -0.0028328 0.2414578 0.2466969 Marcks myristoylated alanine-rich protein kinase C substrate 1275 +/- 140 1506 +/- 125 1333 +/- 57 1365 +/- 179 -0.5733369 0.2463850 0.8707877 Ldha lactate dehydrogenase A 7428 +/- 363 7503 +/- 464 5839 +/- 323 6507 +/- 427 -0.0000080 0.9005206 0.2481737 Map1Lc3B microtubule-associated protein 1 light chain 3 beta 4795 +/- 243 5232 +/- 261 4483 +/- 110 4532 +/- 142 -0.0115719 0.2487294 0.7921388 Ddit4L DNA-damage-inducible transcript 4-like 666 +/- 84 706 +/- 167 657 +/- 67 817 +/- 109 0.3280657 0.8386068 0.2511702 Psat1 phosphoserine aminotransferase 1 2409 +/- 427 2762 +/- 312 2094 +/- 132 2385 +/- 197 -0.1609960 0.5214536 0.2570118 Cs citrate synthase 5441 +/- 289 5701 +/- 369 5162 +/- 218 5594 +/- 286 -0.1627454 0.5927286 0.2644793 Atic 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclo 1046 +/- 49 1113 +/- 28 796 +/- 52 837 +/- 52 -0.0000301 0.2687251 0.5818458 Timm23 translocase of inner mitochondrial membrane 23 homolog (yeast) 1175 +/- 101 1254 +/- 124 1269 +/- 46 1511 +/- 186 0.0670528 0.6322535 0.2687911 Efcbp2 EF-hand calcium binding protein 2 1044 +/- 125 1270 +/- 149 793 +/- 79 1021 +/- 182 -0.0029319 0.2726921 0.2973846 Metap2 methionyl aminopeptidase 2 2709 +/- 177 2941 +/- 165 2681 +/- 192 2974 +/- 167 0.8169028 0.3592035 0.2797346 P2Rxl1 purinergic receptor P2X-like 1, orphan receptor 117 +/- 15 144 +/- 19 144 +/- 14 162 +/- 11 0.2658228 0.2878104 0.3242321 Cgref1 cell growth regulator with EF-hand domain 1 862 +/- 130 980 +/- 56 884 +/- 55 1011 +/- 95 0.7796138 0.4323290 0.2906128 Rps13 ribosomal protein S13 16142 +/- 483 16738 +/- 421 14590 +/- 129 15112 +/- 432 -0.0004701 0.3746461 0.3020581 Snrpn small nuclear ribonucleoprotein polypeptide N 132 +/- 14 163 +/- 31 176 +/- 10 237 +/- 52 0.0177708 0.3858409 0.3183962 Faah fatty acid amide hydrolase 921 +/- 103 1040 +/- 105 1172 +/- 52 1240 +/- 41 0.0008427 0.4358339 0.3273894 Lss lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) 109 +/- 28 144 +/- 20 177 +/- 34 182 +/- 28 0.0320322 0.3303026 0.9065936 Sfpq splicing factor proline/glutamine-rich (polypyrimidine tract binding protein ass 2328 +/- 109 2456 +/- 58 2770 +/- 74 2832 +/- 78 0.0002077 0.3319386 0.5785214 Copb2 Coatomer protein complex, subunit beta 2 (beta prime) 536 +/- 116 636 +/- 54 557 +/- 96 662 +/- 43 0.4268643 0.4608351 0.3504571 Glra2 glycine receptor, alpha 2 147 +/- 22 148 +/- 32 169 +/- 18 224 +/- 52 0.1182836 0.9751751 0.3611267 Ppil3 peptidylprolyl isomerase (cyclophilin)-like 3 1204 +/- 34 1212 +/- 75 1026 +/- 46 1101 +/- 64 -0.0154008 0.9220126 0.3673219 Acbd3 acyl-Coenzyme A binding domain containing 3 163 +/- 16 175 +/- 26 154 +/- 14 178 +/- 21 -0.3586347 0.7225161 0.3782118 Slco1A2 solute carrier organic anion transporter family, member 1A2 187 +/- 36 249 +/- 56 150 +/- 32 164 +/- 21 -0.0974812 0.3788965 0.7230962 Rab8B RAB8B, member RAS oncogene family 397 +/- 38 402 +/- 36 545 +/- 46 601 +/- 43 0.0019020 0.9257873 0.4027558 Mgp matrix Gla protein 741 +/- 134 793 +/- 310 706 +/- 150 900 +/- 180 0.9552698 0.8817384 0.4311350 Plcb4 phospholipase C, beta 4 168 +/- 17 177 +/- 16 198 +/- 6 207 +/- 9 0.0179458 0.7101964 0.4393276 Bsg basigin (Ok blood group) 3168 +/- 363 3280 +/- 262 3443 +/- 131 3625 +/- 188 0.1582191 0.8073385 0.4529086 Grp gastrin-releasing peptide 586 +/- 219 844 +/- 288 2051 +/- 268 2392 +/- 358 0.0015348 0.4937027 0.4688006 Ide insulin-degrading enzyme 665 +/- 21 696 +/- 35 558 +/- 17 572 +/- 22 -0.0001730 0.4716953 0.6080864 Lrp3 low density lipoprotein receptor-related protein 3 1738 +/- 85 1898 +/- 194 1955 +/- 148 2034 +/- 76 0.0495001 0.4749156 0.6468481 Tcf8 transcription factor 8 (represses interleukin 2 expression) 267 +/- 31 295 +/- 33 315 +/- 40 330 +/- 11 0.1234885 0.5466381 0.7237962 Tdg thymine-DNA glycosylase 603 +/- 39 632 +/- 23 612 +/- 15 623 +/- 17 0.8499448 0.5495705 0.6447162 Xrcc1 X-ray repair complementing defective repair in Chinese hamster cells 1 584 +/- 68 627 +/- 18 631 +/- 21 651 +/- 31 0.3635712 0.5666546 0.6009106 Negr1 neuronal growth regulator 1 1940 +/- 226 2083 +/- 141 2266 +/- 136 2279 +/- 43 0.0530881 0.6065945 0.9293982 Folh1 folate hydrolase (prostate-specific membrane antigen) 1 917 +/- 152 1069 +/- 251 549 +/- 87 598 +/- 99 -0.0235439 0.6166617 0.7174146 Lmo3 LIM domain only 3 (rhombotin-like 2) 9466 +/- 961 9899 +/- 747 7430 +/- 667 7767 +/- 555 -0.0280167 0.7296423 0.7064928

S3- Aging related genes in rhesus and rat.xls Page 14 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG Upregulated with Age and Correlated with MSI in Both Regions AASDH 2-aminoadipic 6-semialdehyde dehydrogenase 587 +/- 19 771 +/- 21 501 +/- 29 664 +/- 26 8.64385E-05 0.002318093 0.004617508 0.034790591 ADPRH ADP-ribosylarginine hydrolase 189 +/- 23 309 +/- 20 168 +/- 16 281 +/- 20 0.003010058 0.002340409 0.002813904 0.009052543 AFF3 AF4/FMR2 family, member 3 128 +/- 17 237 +/- 14 224 +/- 20 358 +/- 44 0.000735865 0.034612996 0.001377619 0.000307055 AKR7A3 aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) 722 +/- 43 917 +/- 15 718 +/- 9 959 +/- 30 0.004954826 0.000763437 0.008846722 0.007598488 ATG4B ATG4 autophagy related 4 homolog B (S. cerevisiae) 489 +/- 25 632 +/- 27 517 +/- 23 734 +/- 11 0.00307991 5.64218E-05 0.043959343 0.006532486 ATPBD1B ATP binding domain 1 family, member B 517 +/- 51 773 +/- 37 492 +/- 40 837 +/- 27 0.002910916 7.10449E-05 0.012451055 0.02304461 ATXN2 ataxin 2 1690 +/- 104 2226 +/- 102 1668 +/- 61 2193 +/- 86 0.004376062 0.0013222 0.039174792 0.019668653 BCAP31 B-cell receptor-associated protein 31 1029 +/- 88 1543 +/- 54 860 +/- 54 1436 +/- 92 0.000989636 0.001211166 0.00447701 0.013356191 BIRC6 baculoviral IAP repeat-containing 6 (apollon) 348 +/- 15 458 +/- 21 431 +/- 7 524 +/- 30 0.002066661 0.034165633 0.000871983 0.018599474 C1QC complement component 1, q subcomponent, C chain 541 +/- 44 951 +/- 109 482 +/- 52 755 +/- 55 0.011017819 0.006202431 0.000361247 0.002406633 CAST calpastatin 990 +/- 127 1424 +/- 112 822 +/- 41 1048 +/- 19 0.028764479 0.001579583 0.016179368 0.007630135 CD99 CD99 molecule 895 +/- 110 1907 +/- 171 1138 +/- 143 2051 +/- 28 0.000900323 0.001141378 0.000860348 0.017907846 CHURC1 churchill domain containing 1 2879 +/- 78 3384 +/- 118 2472 +/- 75 2873 +/- 90 0.006513968 0.008506553 0.00139831 0.010048628 CNNM3 Cyclin M3 133 +/- 9 173 +/- 11 136 +/- 17 194 +/- 15 0.015712283 0.030455353 0.012433498 0.047658064 COMT catechol-O-methyltransferase 2761 +/- 143 3402 +/- 147 2879 +/- 124 3569 +/- 121 0.010656279 0.003185201 0.005039179 0.02559968 CTNNB1 Catenin (cadherin-associated protein), beta 1, 88kDa 80 +/- 20 240 +/- 38 98 +/- 21 283 +/- 59 0.006495803 0.031255701 0.010394166 0.001233394 CWF19L2 CWF19-like 2, cell cycle control (S. pombe) 477 +/- 43 740 +/- 86 434 +/- 31 541 +/- 30 0.027047664 0.035306206 0.001702426 0.034630212 DBF4B DBF4 homolog B (S. cerevisiae) 88 +/- 14 172 +/- 19 93 +/- 13 170 +/- 27 0.006619257 0.043190765 0.043322784 0.006960112 ELAC1 elaC homolog 1 (E. coli) 257 +/- 16 328 +/- 22 295 +/- 19 351 +/- 12 0.027760918 0.037583255 0.003573192 0.014227477 EMP2 epithelial membrane protein 2 131 +/- 20 209 +/- 17 138 +/- 17 214 +/- 16 0.01333844 0.011268119 0.020778594 0.015911054 FAIM Fas apoptotic inhibitory molecule 608 +/- 41 920 +/- 63 711 +/- 51 974 +/- 37 0.002737517 0.002460271 0.003273115 0.029247671 FASTK Fas-activated serine/threonine kinase 625 +/- 37 797 +/- 40 549 +/- 14 706 +/- 35 0.010554027 0.00819985 0.048923014 0.00437731 GANC glucosidase, alpha; neutral C 451 +/- 60 883 +/- 120 322 +/- 16 530 +/- 33 0.013551627 0.001369076 0.005156133 0.005970585 GCLM glutamate-cysteine ligase, modifier subunit 771 +/- 36 943 +/- 26 851 +/- 27 1048 +/- 44 0.003664727 0.007250878 0.022972602 0.013004282 GLG1 golgi apparatus protein 1 4000 +/- 666 6938 +/- 706 3906 +/- 536 6715 +/- 578 0.012773863 0.006455356 0.02005048 0.006021599 GMFG glia maturation factor, gamma 412 +/- 31 565 +/- 21 316 +/- 21 420 +/- 14 0.002744673 0.002887507 0.0042369 0.048962988 GRIK1 glutamate receptor, ionotropic, kainate 1 107 +/- 11 355 +/- 42 65 +/- 9 194 +/- 15 0.001556135 0.000176201 0.007775472 0.005943036 GSTT1 Glutathione S-transferase theta 1 180 +/- 17 259 +/- 19 171 +/- 15 240 +/- 13 0.012660553 0.007440094 0.003355471 0.046202777 HNRPA0 Heterogeneous nuclear ribonucleoprotein A0 336 +/- 12 561 +/- 28 394 +/- 18 576 +/- 23 0.000198937 0.000241532 0.009480719 0.019250485 IARS2 isoleucine-tRNA synthetase 2, mitochondrial 656 +/- 36 831 +/- 33 634 +/- 50 784 +/- 31 0.004644977 0.03303207 0.000374872 0.005812832 ICA1L islet cell autoantigen 1,69kDa-like 791 +/- 58 1295 +/- 113 876 +/- 42 1345 +/- 82 0.004674354 0.002172857 0.046687598 0.017191168 LETMD1 LETM1 domain containing 1 392 +/- 31 684 +/- 39 420 +/- 19 632 +/- 54 0.00020745 0.013535878 0.000410661 0.003563626 LYPLAL1 Lysophospholipase-like 1 1724 +/- 216 2920 +/- 69 2065 +/- 232 3308 +/- 85 0.00184455 0.002086311 0.011325788 0.034221821 MAT2B methionine adenosyltransferase II, beta 1924 +/- 121 3545 +/- 199 2623 +/- 156 3789 +/- 141 9.94444E-05 0.000359742 0.007379867 0.004130652 MPEG1 macrophage expressed gene 1 612 +/- 60 826 +/- 70 399 +/- 27 583 +/- 25 0.043255164 0.000737961 0.012235515 0.035965855 MUM1 melanoma associated antigen (mutated) 1 419 +/- 34 814 +/- 85 436 +/- 18 624 +/- 61 0.004201204 0.035077496 0.006394319 0.002899171 NASP Nuclear autoantigenic sperm protein (histone-binding) 466 +/- 56 813 +/- 84 458 +/- 42 736 +/- 35 0.007837765 0.000662374 0.006005902 0.001006533 NEK7 NIMA (never in mitosis gene a)-related kinase 7 2990 +/- 247 3754 +/- 214 2291 +/- 122 2919 +/- 48 0.042078561 0.002479374 0.024529863 0.011491773 NFKBIZ Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta 167 +/- 11 264 +/- 26 150 +/- 6 206 +/- 14 0.011284895 0.012170385 0.000138843 0.025442153 NHP2L1 NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) 59 +/- 14 219 +/- 15 65 +/- 8 167 +/- 16 1.60482E-05 0.001334391 8.78855E-05 0.045871821 PA2G4 proliferation-associated 2G4, 38kDa 712 +/- 69 939 +/- 45 885 +/- 51 1182 +/- 44 0.023198878 0.001751756 0.029756135 0.00043482 PACRG PARK2 co-regulated 1071 +/- 37 1394 +/- 73 893 +/- 44 1132 +/- 74 0.004923138 0.028089353 0.000589869 0.00632666 PAPD1 PAP associated domain containing 1 886 +/- 44 1400 +/- 87 1045 +/- 67 1422 +/- 80 0.000963235 0.006476203 0.002257992 0.002326517

S4- MSI correlated and non-correlated ARGs.xls Page 1 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG PBX1 Pre-B-cell leukemia transcription factor 1 1716 +/- 118 2769 +/- 217 1455 +/- 111 2238 +/- 91 0.002974631 0.000413004 0.004411418 0.006128792 PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 531 +/- 38 759 +/- 35 440 +/- 25 533 +/- 18 0.001333503 0.014641099 0.00556195 0.013500595 PHOSPHO2 phosphatase, orphan 2 671 +/- 78 1066 +/- 59 648 +/- 36 1124 +/- 54 0.002665896 0.000135035 0.030363144 0.006634282 PSMB9 proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) 262 +/- 10 349 +/- 12 233 +/- 6 295 +/- 8 0.000275211 0.000367261 0.008896198 0.033050982 QTRT1 queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) 256 +/- 22 498 +/- 21 323 +/- 31 587 +/- 36 1.48171E-05 0.0004756 0.005966107 0.001649222 RAB28 RAB28, member RAS oncogene family 757 +/- 67 1355 +/- 52 860 +/- 38 1404 +/- 71 4.71678E-05 0.000443803 0.005323197 0.009386422 RAB8A RAB8A, member RAS oncogene family 660 +/- 25 807 +/- 25 552 +/- 11 656 +/- 19 0.002189185 0.002420763 0.004868392 0.000351065 RBM3 RNA binding motif (RNP1, RRM) protein 3 669 +/- 111 1358 +/- 95 805 +/- 127 1331 +/- 55 0.000867397 0.007144176 0.021397593 0.0454136 RBM5 RNA binding motif protein 5 1495 +/- 88 1813 +/- 55 1547 +/- 80 1848 +/- 62 0.014794842 0.016372199 0.02800611 0.025389931 RPL13 ribosomal protein L13 3331 +/- 242 4584 +/- 286 2546 +/- 122 3520 +/- 104 0.007698486 0.000188507 0.008723084 0.005954513 RPS15A ribosomal protein S15a 14626 +/- 477 16659 +/- 556 11527 +/- 341 13684 +/- 211 0.019978008 0.000641867 0.018752943 0.008801629 RRBP1 ribosome binding protein 1 homolog 180kDa (dog) 119 +/- 18 186 +/- 24 125 +/- 7 169 +/- 11 0.049489585 0.010814197 0.018622373 0.01476604 RTCD1 RNA terminal phosphate cyclase domain 1 145 +/- 9 194 +/- 12 137 +/- 5 174 +/- 10 0.008233371 0.017040036 0.012001762 0.000556839 SAP18 Sin3A-associated protein, 18kDa 2878 +/- 140 4280 +/- 172 2600 +/- 139 3721 +/- 168 0.000101626 0.000789111 9.32216E-05 0.005042798 SEC23IP SEC23 interacting protein 945 +/- 34 1092 +/- 23 868 +/- 39 1118 +/- 42 0.006532663 0.001978948 0.017475982 0.036360421 SEPT8 septin 8 2774 +/- 489 5315 +/- 965 1763 +/- 94 3266 +/- 249 0.049189087 0.002204603 0.014303119 0.003895876 SGCE sarcoglycan, epsilon 754 +/- 60 1219 +/- 49 868 +/- 51 1204 +/- 28 0.000157526 0.000483119 0.003289368 0.006452203 SLC11A2 solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 475 +/- 88 1020 +/- 94 461 +/- 69 952 +/- 81 0.00174478 0.001506198 0.009127034 0.010460024 SLC15A4 Solute carrier family 15, member 4 630 +/- 46 885 +/- 55 671 +/- 24 756 +/- 14 0.005337203 0.014981656 0.01299924 0.036015026 SLC4A7 solute carrier family 4, sodium bicarbonate cotransporter, member 7 1387 +/- 232 3599 +/- 647 1815 +/- 290 4114 +/- 614 0.017154071 0.015697579 0.028831018 0.01155934 SSBP2 Single-stranded DNA binding protein 2 148 +/- 42 473 +/- 63 131 +/- 36 339 +/- 32 0.002159678 0.001879609 0.004952731 0.00272126 SURB7 SRB7 suppressor of RNA polymerase B homolog (yeast) 1618 +/- 167 2473 +/- 241 1436 +/- 158 2200 +/- 169 0.017365446 0.009589969 0.026485383 0.023674629 TFPI tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) 202 +/- 14 337 +/- 20 145 +/- 11 265 +/- 18 0.000360429 0.001001625 0.006258452 0.007355722 TFPT TCF3 (E2A) fusion partner (in childhood Leukemia) 369 +/- 36 499 +/- 34 283 +/- 25 365 +/- 25 0.024337442 0.042480101 0.048447198 0.011455106 THEDC1 thioesterase domain containing 1 35 +/- 9 287 +/- 70 65 +/- 11 160 +/- 25 0.015278438 0.014874365 0.000760961 0.013168188 TMEM80 transmembrane protein 80 995 +/- 50 1270 +/- 42 785 +/- 32 942 +/- 27 0.001974292 0.004549828 0.002520586 0.023382926 TMEM97 transmembrane protein 97 1042 +/- 36 1322 +/- 88 914 +/- 45 1135 +/- 30 0.023149686 0.003205269 0.017416421 0.01963856 TP53RK TP53 regulating kinase 189 +/- 12 227 +/- 11 170 +/- 2 221 +/- 13 0.037550082 0.014506803 0.009844555 5.07202E-06 TRAK2 trafficking protein, kinesin binding 2 2695 +/- 155 3432 +/- 218 2515 +/- 153 2941 +/- 67 0.022400534 0.039325312 0.01139918 0.04426098 TRAPPC2 trafficking protein particle complex 2 1738 +/- 51 1929 +/- 62 1792 +/- 47 1967 +/- 42 0.038920327 0.020945528 0.044279379 0.036832665 TUFT1 tuftelin 1 796 +/- 100 1318 +/- 115 650 +/- 8 921 +/- 37 0.006735938 0.001405156 0.011219071 0.031659984 UBE2L6 ubiquitin-conjugating enzyme E2L 6 690 +/- 43 965 +/- 37 708 +/- 47 941 +/- 37 0.000689307 0.003762354 0.004088708 0.005934586 ZKSCAN1 zinc finger with KRAB and SCAN domains 1 1152 +/- 95 1766 +/- 191 984 +/- 18 1287 +/- 34 0.022665788 0.000206109 0.007582694 0.000442514 ZNF596 zinc finger protein 596 360 +/- 12 434 +/- 25 392 +/- 8 446 +/- 11 0.029173479 0.004145729 0.003779054 0.001302882 ZNF622 zinc finger protein 622 946 +/- 28 1045 +/- 22 741 +/- 18 848 +/- 18 0.021926133 0.002301878 0.021069267 0.049957309 ZNF83 zinc finger protein 83 385 +/- 25 545 +/- 35 388 +/- 21 477 +/- 31 0.004679458 0.046906063 0.000678943 0.044284686 Downregulated with Age and Correlated with MSI in Both Regions ACCN1 amiloride-sensitive cation channel 1, neuronal (degenerin) 1925 +/- 136 1421 +/- 164 1179 +/- 59 900 +/- 42 -0.040656154 -0.004391613 0.027325315 0.012148097 AP2B1 adaptor-related protein complex 2, beta 1 subunit 7691 +/- 354 5572 +/- 446 5527 +/- 121 4060 +/- 189 -0.004297353 -0.000323074 0.028797532 0.027387172 AP4S1 adaptor-related protein complex 4, sigma 1 subunit 336 +/- 50 195 +/- 7 440 +/- 40 279 +/- 34 -0.035594454 -0.012957804 0.022347191 0.047586785 B4GALT3 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3 1509 +/- 46 1313 +/- 50 1304 +/- 35 1196 +/- 29 -0.016365607 -0.043078845 0.005971498 0.010920506 CCL27 chemokine (C-C motif) ligand 27 350 +/- 36 242 +/- 23 335 +/- 21 227 +/- 16 -0.034546636 -0.002895241 0.036856456 0.003753417 CSNK1G2 casein kinase 1, gamma 2 464 +/- 48 332 +/- 27 622 +/- 27 496 +/- 28 -0.044696832 -0.010409097 0.03554518 0.006842157 DNAJB5 DnaJ (Hsp40) homolog, subfamily B, member 5 1205 +/- 78 888 +/- 41 1163 +/- 41 974 +/- 62 -0.007384671 -0.038186221 0.002989718 0.044401411

S4- MSI correlated and non-correlated ARGs.xls Page 2 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG DOC2B double C2-like domains, beta 258 +/- 27 166 +/- 20 253 +/- 19 182 +/- 20 -0.023574925 -0.030195503 0.007181465 0.020460639 DOK6 docking protein 6 1184 +/- 243 182 +/- 52 1228 +/- 38 204 +/- 68 -0.008389508 -6.71455E-06 0.021924741 0.016210785 ELAVL3 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) 189 +/- 16 110 +/- 15 287 +/- 18 170 +/- 21 -0.005033619 -0.002435542 0.013616025 0.031892021 FYN FYN oncogene related to SRC, FGR, YES 243 +/- 42 94 +/- 25 298 +/- 27 175 +/- 27 -0.01481736 -0.010902184 0.008592589 0.007593408 GNG2 guanine nucleotide binding protein (G protein), gamma 2 7405 +/- 371 5485 +/- 393 7908 +/- 317 6914 +/- 174 -0.005280976 -0.026402451 0.01485687 0.038332353 GNPTG N-acetylglucosamine-1-phosphate transferase, gamma subunit 1259 +/- 45 970 +/- 51 1269 +/- 41 946 +/- 36 -0.001691422 -0.000241697 0.038246233 0.016103241 GOLT1B golgi transport 1 homolog B (S. cerevisiae) 971 +/- 73 734 +/- 56 960 +/- 48 651 +/- 52 -0.029642906 -0.002021516 0.037834422 0.005539233 GPATC2 G patch domain containing 2 364 +/- 9 306 +/- 7 390 +/- 22 307 +/- 16 -0.000614856 -0.014567746 0.015153675 0.003991556 HAGH hydroxyacylglutathione hydrolase 3456 +/- 243 2690 +/- 123 2867 +/- 137 2258 +/- 72 -0.024496714 -0.00511899 0.03013287 0.021901063 HAX1 HCLS1 associated protein X-1 2262 +/- 81 1987 +/- 38 2212 +/- 35 1989 +/- 78 -0.017283174 -0.042094235 0.03294257 0.017801407 HSD17B6 hydroxysteroid (17-beta) dehydrogenase 6 3312 +/- 301 2181 +/- 99 2842 +/- 167 1796 +/- 97 -0.011556512 -0.000670105 0.024063117 0.011679094 HSPE1 heat shock 10kDa protein 1 (chaperonin 10) 3558 +/- 135 3064 +/- 138 3300 +/- 90 2930 +/- 94 -0.028254719 -0.019469481 0.046640491 0.030589904 KPNA3 karyopherin alpha 3 (importin alpha 4) 2725 +/- 82 2246 +/- 35 2709 +/- 42 2343 +/- 59 -0.001186814 -0.001241669 0.006676681 0.031217584 MARCH6 membrane-associated ring finger (C3HC4) 6 1489 +/- 19 1154 +/- 34 1345 +/- 50 1122 +/- 25 -2.87307E-05 -0.004756709 0.00504008 0.044317409 NETO2 neuropilin (NRP) and tolloid (TLL)-like 2 2261 +/- 64 1727 +/- 92 1797 +/- 68 1378 +/- 100 -0.00101761 -0.00954415 0.005995911 0.022443801 NMRAL1 NmrA-like family domain containing 1 1470 +/- 75 1003 +/- 79 1214 +/- 54 797 +/- 29 -0.001640265 -0.000185534 0.030343869 0.008535776 NOL8 nucleolar protein 8 1828 +/- 50 1420 +/- 66 1771 +/- 73 1225 +/- 62 -0.00076339 -0.000309776 0.01342445 0.007196184 OSTalpha organic solute transporter alpha 185 +/- 40 66 +/- 8 151 +/- 24 54 +/- 13 -0.028624227 -0.007413788 0.017158669 0.046107008 PAIP1 poly(A) binding protein interacting protein 1 2718 +/- 182 2080 +/- 74 3154 +/- 104 2326 +/- 67 -0.015323642 -0.000135552 0.031098988 0.014158099 PCGF2 polycomb group ring finger 2 253 +/- 17 158 +/- 15 362 +/- 10 234 +/- 26 -0.001817183 -0.005282196 0.043885923 0.03964353 PDXP pyridoxal (pyridoxine, vitamin B6) phosphatase 5584 +/- 267 4404 +/- 240 5656 +/- 156 4968 +/- 141 -0.00838954 -0.009630668 0.000424707 0.014743271 PRELP proline/arginine-rich end leucine-rich repeat protein 1546 +/- 52 1157 +/- 104 1515 +/- 84 1157 +/- 70 -0.011628872 -0.009821272 0.014033141 0.004110821 PYCR1 pyrroline-5-carboxylate reductase 1 547 +/- 59 391 +/- 22 506 +/- 30 365 +/- 21 -0.04501709 -0.004591689 0.037461291 0.046361606 RAD50 RAD50 homolog (S. cerevisiae) 416 +/- 27 289 +/- 24 390 +/- 21 293 +/- 18 -0.005346262 -0.006815895 0.017455681 0.006224269 RTN3 reticulon 3 21006 +/- 1230 13544 +/- 1159 20556 +/- 206 13859 +/- 1090 -0.001314002 -0.002946323 0.04701055 0.044878675 RYR1 ryanodine receptor 1 (skeletal) 1032 +/- 27 722 +/- 33 993 +/- 101 590 +/- 54 -3.07963E-05 -0.008597255 0.000352985 0.025259361 SKIV2L superkiller viralicidic activity 2-like (S. cerevisiae) 624 +/- 40 474 +/- 21 631 +/- 36 490 +/- 27 -0.011363843 -0.01219067 0.006765284 0.022301374 SNAP25 Synaptosomal-associated protein, 25kDa 583 +/- 70 304 +/- 41 826 +/- 34 518 +/- 59 -0.008466436 -0.003209955 0.011288112 0.012330398 SOCS1 suppressor of cytokine signaling 1 193 +/- 12 114 +/- 15 214 +/- 14 159 +/- 18 -0.002148166 -0.038542504 0.000649267 0.001437317 SORBS1 sorbin and SH3 domain containing 1 688 +/- 45 536 +/- 41 818 +/- 42 620 +/- 39 -0.032043767 -0.007102991 0.044553618 0.004580935 SSX2IP synovial sarcoma, X breakpoint 2 interacting protein 256 +/- 17 190 +/- 21 218 +/- 7 166 +/- 12 -0.036496511 -0.007626411 0.032746678 0.009024617 STARD3NL STARD3 N-terminal like 1387 +/- 85 942 +/- 16 1203 +/- 86 834 +/- 52 -0.003025776 -0.006157765 0.007787543 0.044308108 SUGT1L1 SGT1, suppressor of G2 allele of SKP1 like 1 (S. cerevisiae) 240 +/- 35 130 +/- 4 288 +/- 39 162 +/- 19 -0.024886781 -0.021919699 0.018449494 0.037105756 SUPT7L suppressor of Ty 7 (S. cerevisiae)-like 547 +/- 33 403 +/- 23 594 +/- 42 454 +/- 21 -0.005810865 -0.019970004 0.012704977 0.028654792 TBCA tubulin-specific chaperone a 3775 +/- 186 3218 +/- 114 3953 +/- 59 3483 +/- 98 -0.033245402 -0.004841134 0.028952345 0.023663811 THUMPD3 THUMP domain containing 3 1272 +/- 40 1006 +/- 65 1229 +/- 30 921 +/- 79 -0.007846011 -0.014490502 0.030597041 0.008000834 THY1 Thy-1 cell surface antigen 9156 +/- 962 5509 +/- 551 8393 +/- 478 6379 +/- 427 -0.01109214 -0.011919883 0.004873262 0.012529639 TMEM100 transmembrane protein 100 1126 +/- 85 764 +/- 92 810 +/- 93 482 +/- 73 -0.016281587 -0.021696694 0.002098092 0.012211156 TOR1B torsin family 1, member B (torsin B) 824 +/- 61 605 +/- 42 797 +/- 27 668 +/- 33 -0.015886425 -0.016497061 0.028667108 0.018547922 TRAK1 trafficking protein, kinesin binding 1 827 +/- 63 630 +/- 58 1039 +/- 46 885 +/- 23 -0.044995521 -0.01860509 0.016632856 0.012422834 UBE1DC1 ubiquitin-activating enzyme E1-domain containing 1 944 +/- 81 433 +/- 60 1060 +/- 122 436 +/- 35 -0.000650153 -0.00297969 0.014896329 0.021042621 XKR4 XK, Kell blood group complex subunit-related family, member 4 1695 +/- 77 1381 +/- 64 1756 +/- 52 1344 +/- 38 -0.010927465 -0.000143588 0.006413121 0.007877832 ZNF197 zinc finger protein 197 370 +/- 35 200 +/- 10 492 +/- 28 326 +/- 18 -0.003679985 -0.000981967 0.007048488 0.016949976 ZNF200 zinc finger protein 200 442 +/- 11 377 +/- 17 482 +/- 19 399 +/- 22 -0.011260571 -0.018821499 0.003000017 0.015180382

S4- MSI correlated and non-correlated ARGs.xls Page 3 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG ZNF285 zinc finger protein 285 234 +/- 6 184 +/- 8 245 +/- 9 194 +/- 13 -0.000899268 -0.014432968 0.003187189 0.002960055 ZNF586 zinc finger protein 586 316 +/- 21 232 +/- 26 438 +/- 20 279 +/- 34 -0.031862631 -0.005803159 0.035304275 0.001748864 ZNF626 Zinc finger protein 626 262 +/- 29 140 +/- 14 248 +/- 23 150 +/- 12 -0.006163 -0.00592928 0.036840866 0.041037013 ZNF697 zinc finger protein 697 458 +/- 22 383 +/- 9 394 +/- 27 303 +/- 1 -0.018619432 -0.020788817 0.026309799 0.033516951 ZNF9 zinc finger protein 9 6431 +/- 219 5316 +/- 196 5728 +/- 139 4867 +/- 76 -0.003619593 -0.000741603 0.031262656 0.011434175 ZNHIT3 zinc finger, HIT type 3 1626 +/- 109 1266 +/- 38 1613 +/- 77 1374 +/- 14 -0.019780045 -0.026213431 0.026900181 0.038943782 Upregulated with Age and Correlated with MSI in CA Region ACAA2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) 589 +/- 56 854 +/- 62 529 +/- 31 645 +/- 33 0.009788915 0.03015292 0.000593989 0.107868689 ACACB acetyl-Coenzyme A carboxylase beta 124 +/- 17 175 +/- 13 138 +/- 12 164 +/- 12 0.037635496 0.152219196 0.029375417 0.335873349 ADA adenosine deaminase 265 +/- 19 352 +/- 29 341 +/- 51 373 +/- 25 0.033145219 0.593304815 0.033036236 0.586496456 ADI1 acireductone dioxygenase 1 841 +/- 61 1306 +/- 144 924 +/- 99 1052 +/- 80 0.02136589 0.342331102 0.041146655 0.850211246 AGPAT3 1-acylglycerol-3-phosphate O-acyltransferase 3 124 +/- 10 226 +/- 20 169 +/- 8 232 +/- 26 0.002439272 0.066575849 0.007768695 0.08481765 ALDH3B1 aldehyde dehydrogenase 3 family, member B1 147 +/- 9 263 +/- 37 91 +/- 15 124 +/- 5 0.024390959 0.084643861 0.028408298 0.150807304 ALKBH8 alkB, alkylation repair homolog 8 (E. coli) 473 +/- 41 658 +/- 40 571 +/- 39 657 +/- 38 0.009148021 0.144294944 0.026047021 0.18025115 ANAPC10 anaphase promoting complex subunit 10 140 +/- 9 189 +/- 9 212 +/- 8 217 +/- 21 0.003833009 0.804791569 0.00428111 0.167121141 ANAPC13 anaphase promoting complex subunit 13 1133 +/- 61 1351 +/- 62 1288 +/- 122 1286 +/- 100 0.031286236 -0.989155712 0.045478348 0.467774274 ANGPT1 angiopoietin 1 1046 +/- 93 1388 +/- 95 1366 +/- 231 1407 +/- 71 0.028121722 0.87072691 0.008560379 0.993305479 ANKHD1 ankyrin repeat and KH domain containing 1 870 +/- 60 1083 +/- 30 945 +/- 57 1001 +/- 44 0.014560802 0.458340411 0.006079233 0.436999036 ANKIB1 ankyrin repeat and IBR domain containing 1 1928 +/- 106 2973 +/- 269 1694 +/- 82 1917 +/- 65 0.009743035 0.063334129 9.00003E-05 0.083908189 ANKMY1 ankyrin repeat and MYND domain containing 1 80 +/- 6 123 +/- 12 89 +/- 10 110 +/- 19 0.012491451 0.377353349 0.033130446 0.180486154 ANKRD11 ankyrin repeat domain 11 147 +/- 24 240 +/- 28 262 +/- 22 250 +/- 72 0.030712078 -0.879093938 0.013910307 0.339598862 ANKS1B ankyrin repeat and sterile alpha motif domain containing 1B 7067 +/- 478 8852 +/- 493 7058 +/- 279 8534 +/- 485 0.026588622 0.035708506 0.007534706 0.11313409 APBB1IP amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein 146 +/- 16 200 +/- 16 146 +/- 13 166 +/- 11 0.035110391 0.285012872 0.014207961 0.667314536 API5 apoptosis inhibitor 5 1659 +/- 24 1825 +/- 54 1812 +/- 42 1906 +/- 76 0.026573497 0.322472314 0.01898114 0.055193902 AQP4 aquaporin 4 3429 +/- 247 4405 +/- 244 3780 +/- 277 3499 +/- 199 0.018268701 -0.431324868 0.013753143 0.41633609 ARPC1B actin related protein 2/3 complex, subunit 1B, 41kDa 265 +/- 16 338 +/- 19 206 +/- 20 235 +/- 20 0.014148443 0.321993503 0.002173212 0.707297159 ARRB1 arrestin, beta 1 380 +/- 17 456 +/- 27 349 +/- 27 389 +/- 28 0.042410309 0.331062491 0.040432401 0.682824889 ASB8 ankyrin repeat and SOCS box-containing 8 524 +/- 37 698 +/- 46 630 +/- 54 667 +/- 53 0.015617961 0.635975754 0.017202233 0.353438495 ATF1 Activating transcription factor 1 660 +/- 47 833 +/- 36 824 +/- 43 894 +/- 69 0.016520112 0.413931617 0.01718043 0.360255937 ATG12 ATG12 autophagy related 12 homolog (S. cerevisiae) 1361 +/- 96 1868 +/- 140 1316 +/- 125 1632 +/- 120 0.015757733 0.101921368 0.000609303 0.020707043 AZI2 5-azacytidine induced 2 394 +/- 23 458 +/- 16 409 +/- 24 439 +/- 10 0.048244395 0.292642712 0.033614731 0.353042543 B2M beta-2-microglobulin 7946 +/- 519 10622 +/- 678 6616 +/- 572 9837 +/- 646 0.011496581 0.005147291 0.04236259 0.105180339 bA16L21.2.1 DnaJ-like protein 465 +/- 29 601 +/- 37 472 +/- 48 628 +/- 64 0.016292197 0.086857717 0.007434237 0.021214801 BBS10 Bardet-Biedl syndrome 10 654 +/- 36 800 +/- 32 792 +/- 37 735 +/- 48 0.012777651 -0.374215564 0.004116063 0.315339658 BPNT1 3'(2'), 5'-bisphosphate nucleotidase 1 161 +/- 18 269 +/- 23 210 +/- 23 267 +/- 28 0.00488431 0.15653575 0.01694033 0.034851874 BRCC2 BRCC2 260 +/- 26 370 +/- 35 220 +/- 11 242 +/- 10 0.032344576 0.172531744 0.008758521 0.316360383 BRD8 bromodomain containing 8 2792 +/- 155 3452 +/- 144 2758 +/- 92 3162 +/- 229 0.011073619 0.159508247 0.010020377 0.057172994 BTF3 basic transcription factor 3 1055 +/- 87 1438 +/- 101 1121 +/- 86 1351 +/- 104 0.01682459 0.126082617 0.001567984 0.030301635 BTN2A2 butyrophilin, subfamily 2, member A2 158 +/- 20 239 +/- 24 168 +/- 18 199 +/- 14 0.026219693 0.201096297 0.049364808 0.650402248 BZRP benzodiazapine receptor (peripheral) 565 +/- 74 798 +/- 62 374 +/- 21 504 +/- 36 0.037740141 0.018487277 0.021493421 0.101408274 CALD1 caldesmon 1 84 +/- 6 130 +/- 15 132 +/- 8 136 +/- 6 0.025170472 0.710226139 0.002200006 0.752042955 CAPN7 calpain 7 580 +/- 29 674 +/- 27 693 +/- 37 800 +/- 39 0.039422033 0.081335838 0.046624604 0.26677097 CBR4 Carbonyl reductase 4 86 +/- 10 138 +/- 8 105 +/- 19 109 +/- 10 0.002183177 0.871819196 0.000688489 0.901672561 CCAR1 Cell division cycle and apoptosis regulator 1 149 +/- 19 225 +/- 17 200 +/- 14 216 +/- 12 0.014320053 0.403648957 0.020249837 0.858094474

S4- MSI correlated and non-correlated ARGs.xls Page 4 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG CCDC98 coiled-coil domain containing 98 121 +/- 10 163 +/- 15 95 +/- 14 126 +/- 13 0.044275178 0.141438089 0.017969572 0.319485977 CCNA1 cyclin A1 165 +/- 10 276 +/- 37 154 +/- 14 177 +/- 31 0.030112623 0.505994277 0.0100349 0.554280904 CD52 CD52 molecule 303 +/- 58 621 +/- 52 251 +/- 51 388 +/- 37 0.002332509 0.057454068 0.010007153 0.220588907 CD74 CD74 molecule, major histocompatibility complex, class II invariant chain 1810 +/- 283 3714 +/- 547 1215 +/- 171 2317 +/- 340 0.016152974 0.027628935 0.007913266 0.104936148 CENPJ centromere protein J 96 +/- 18 264 +/- 61 97 +/- 12 311 +/- 82 0.040178199 0.05831503 0.007209935 0.004901223 CEPT1 choline/ethanolamine phosphotransferase 1 157 +/- 10 233 +/- 10 158 +/- 12 186 +/- 14 0.000353143 0.177277119 0.002778644 0.298488707 CES2 carboxylesterase 2 (intestine, liver) 171 +/- 23 239 +/- 16 196 +/- 22 188 +/- 19 0.036610533 -0.781636953 0.006473044 0.995829569 CHCHD3 coiled-coil-helix-coiled-coil-helix domain containing 3 2026 +/- 56 2199 +/- 30 2231 +/- 90 2360 +/- 104 0.026341706 0.377635721 0.0127559 0.521936704 CKS1B CDC28 protein kinase regulatory subunit 1B 822 +/- 24 945 +/- 35 686 +/- 28 763 +/- 14 0.018041856 0.041455361 0.045112677 0.247541605 CLDN15 claudin 15 352 +/- 48 618 +/- 92 272 +/- 21 393 +/- 56 0.03551773 0.099514789 0.014485933 0.038621587 CLEC4A C-type lectin domain family 4, member A 101 +/- 9 155 +/- 10 108 +/- 13 131 +/- 18 0.002772838 0.334356874 0.004178483 0.880775886 CNTN1 contactin 1 1106 +/- 69 1435 +/- 87 1088 +/- 58 1302 +/- 69 0.014910358 0.04357938 0.008978749 0.206808102 CNTNAP4 contactin associated protein-like 4 870 +/- 94 1300 +/- 106 849 +/- 67 946 +/- 93 0.012604945 0.424398341 0.002206251 0.720818022 COG2 Component of oligomeric golgi complex 2 120 +/- 4 171 +/- 15 148 +/- 17 181 +/- 22 0.016373405 0.261060342 0.035706992 0.411789933 CRI1 CREBBP/EP300 inhibitor 1 2595 +/- 106 2983 +/- 108 2958 +/- 141 2891 +/- 157 0.028134869 -0.757243148 0.025830156 0.984512268 CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa 197 +/- 12 461 +/- 85 228 +/- 18 391 +/- 98 0.025739796 0.174307676 0.001970168 0.05375377 CSF2RB colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) 151 +/- 7 191 +/- 15 133 +/- 12 133 +/- 8 0.045124335 -0.994117337 0.000869122 0.775029089 CSPP1 centrosome and spindle pole associated protein 1 281 +/- 13 401 +/- 33 322 +/- 36 395 +/- 48 0.012670568 0.257417295 0.000107694 0.082430289 CTSS cathepsin S 157 +/- 19 238 +/- 20 157 +/- 21 194 +/- 33 0.01377976 0.371197789 0.034324596 0.516011545 CUL5 cullin 5 2810 +/- 145 3330 +/- 140 3029 +/- 94 3332 +/- 99 0.027387502 0.054928152 0.035552916 0.038643562 CWF19L1 CWF19-like 1, cell cycle control (S. pombe) 145 +/- 13 207 +/- 12 192 +/- 13 214 +/- 15 0.006543389 0.297555611 0.010032403 0.985792295 DDB2 damage-specific DNA binding protein 2, 48kDa 98 +/- 11 160 +/- 9 88 +/- 9 129 +/- 8 0.001531774 0.005825251 0.002332177 0.056883278 DDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 600 +/- 31 706 +/- 25 583 +/- 35 620 +/- 21 0.025995402 0.387283312 0.00953505 0.521246735 DIP2C DIP2 disco-interacting protein 2 homolog C (Drosophila) 336 +/- 30 418 +/- 15 445 +/- 23 481 +/- 54 0.04544593 0.560879845 0.032243888 0.230316573 DLEU8 deleted in lymphocytic leukemia 8 330 +/- 44 467 +/- 42 228 +/- 18 277 +/- 20 0.049571778 0.100475198 0.014284086 0.096093547 DNAJA5 DnaJ homology subfamily A member 5 145 +/- 11 219 +/- 23 120 +/- 15 134 +/- 16 0.022337942 0.546183961 0.021867055 0.970190834 DST dystonin 236 +/- 21 370 +/- 38 341 +/- 33 339 +/- 42 0.015105816 -0.977157999 0.006460389 0.365223341 DUSP16 dual specificity phosphatase 16 390 +/- 59 565 +/- 40 382 +/- 19 384 +/- 22 0.038148659 0.953695592 0.03532716 0.884047142 EDD1 E3 ubiquitin protein ligase, HECT domain containing, 1 880 +/- 28 980 +/- 21 1000 +/- 50 955 +/- 46 0.017732157 -0.513781775 0.047109262 0.485600238 EFCAB2 EF-hand calcium binding domain 2 1046 +/- 47 1331 +/- 56 1121 +/- 95 1272 +/- 82 0.003111712 0.260352779 0.009235402 0.753171891 EI24 etoposide induced 2.4 mRNA 1967 +/- 197 2517 +/- 46 2093 +/- 202 2274 +/- 91 0.037471438 0.441746788 0.039190826 0.210107781 EIF1AX eukaryotic translation initiation factor 1A, X-linked 237 +/- 22 1333 +/- 96 475 +/- 143 1352 +/- 99 4.71003E-05 0.00082015 0.000225341 0.052504669 EIF2AK4 eukaryotic translation initiation factor 2 alpha kinase 4 413 +/- 20 484 +/- 18 343 +/- 27 326 +/- 8 0.026366173 -0.570407514 0.008715799 0.509334725 EIF5B eukaryotic translation initiation factor 5B 746 +/- 19 848 +/- 36 734 +/- 20 791 +/- 15 0.037126904 0.052223377 0.002047749 0.055285422 ELK3 ELK3, ETS-domain protein (SRF accessory protein 2) 355 +/- 29 436 +/- 19 346 +/- 23 388 +/- 29 0.04211483 0.281534946 0.027550579 0.479047216 ELTD1 EGF, latrophilin and seven transmembrane domain containing 1 216 +/- 15 319 +/- 20 190 +/- 19 228 +/- 17 0.002299519 0.165249698 0.000525359 0.239435816 EML4 echinoderm microtubule associated protein like 4 276 +/- 12 333 +/- 21 362 +/- 36 401 +/- 18 0.046479179 0.35485968 0.006840896 0.506707698 EP400 E1A binding protein p400 812 +/- 48 952 +/- 33 925 +/- 77 961 +/- 76 0.038966412 0.741596791 0.008817697 0.30062797 EPB41L5 erythrocyte membrane protein band 4.1 like 5 80 +/- 8 128 +/- 8 94 +/- 11 112 +/- 3 0.002336984 0.173635932 0.001242356 0.363423256 EPS8 epidermal growth factor receptor pathway substrate 8 1375 +/- 55 1537 +/- 28 1326 +/- 75 1257 +/- 47 0.033353369 -0.451631986 0.040372544 0.149476439 ERBB2IP erbb2 interacting protein 1656 +/- 204 2474 +/- 294 1821 +/- 122 2048 +/- 58 0.048527287 0.13714293 0.005407336 0.231457383 EXOSC10 exosome component 10 957 +/- 62 1170 +/- 42 800 +/- 35 895 +/- 36 0.020228702 0.092599271 0.029689182 0.190432265 FCGR3B Fc fragment of IgG, low affinity IIIb, receptor (CD16b) 158 +/- 14 222 +/- 21 125 +/- 11 167 +/- 20 0.030722125 0.112317008 0.00835231 0.003997434 FCHO2 FCH domain only 2 843 +/- 52 1043 +/- 48 843 +/- 51 914 +/- 52 0.017743407 0.35672621 0.005791581 0.267698896

S4- MSI correlated and non-correlated ARGs.xls Page 5 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG FKBP3 FK506 binding protein 3, 25kDa 2550 +/- 62 2815 +/- 70 2687 +/- 106 2837 +/- 134 0.018288248 0.403422708 0.003406601 0.329754084 FLJ00038 CXYorf1-related protein 477 +/- 9 660 +/- 29 559 +/- 32 584 +/- 37 0.001017878 0.630895092 0.004518516 0.642275137 GAMT guanidinoacetate N-methyltransferase 1022 +/- 43 1199 +/- 29 1147 +/- 61 1359 +/- 156 0.009401011 0.261073021 0.009784573 0.178100618 GGPS1 Geranylgeranyl diphosphate synthase 1 96 +/- 10 181 +/- 17 152 +/- 9 155 +/- 10 0.002501562 0.803335947 0.001280019 0.707845942 GJA1 gap junction protein, alpha 1, 43kDa (connexin 43) 11658 +/- 328 13350 +/- 420 12800 +/- 751 13085 +/- 225 0.010669343 0.728814328 0.013546253 0.852820526 GLA galactosidase, alpha 400 +/- 19 475 +/- 12 357 +/- 11 391 +/- 12 0.010141907 0.061628151 0.013596409 0.348694601 GLDN gliomedin 316 +/- 58 508 +/- 25 308 +/- 25 409 +/- 21 0.019579409 0.013554132 0.027041741 0.13033409 GNB2L1 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 8227 +/- 314 9584 +/- 311 7947 +/- 439 9269 +/- 228 0.011821474 0.030345679 0.037887298 0.095970664 GOLGA2 golgi autoantigen, golgin subfamily a, 2 80 +/- 6 112 +/- 6 118 +/- 19 140 +/- 9 0.004714028 0.328757004 0.019105226 0.591558507 GOLGA4 golgi autoantigen, golgin subfamily a, 4 158 +/- 20 223 +/- 19 236 +/- 13 257 +/- 20 0.040190229 0.414472874 0.019482734 0.154938894 GON4L gon-4-like (C.elegans) 260 +/- 13 344 +/- 16 310 +/- 44 351 +/- 20 0.002339183 0.428238667 0.001184281 0.778380809 GPATC1 G patch domain containing 1 206 +/- 10 251 +/- 12 231 +/- 21 230 +/- 9 0.01685538 -0.991300297 0.0059077 0.960845591 GPM6B glycoprotein M6B 5149 +/- 133 6879 +/- 254 6195 +/- 386 7340 +/- 323 0.000392904 0.049273197 0.007491822 0.472334046 GPR89A G protein-coupled receptor 89A 470 +/- 16 578 +/- 23 474 +/- 20 537 +/- 29 0.004337106 0.112575075 0.013336693 0.381691401 GPRC5B G protein-coupled receptor, family C, group 5, member B 2685 +/- 255 3426 +/- 164 2738 +/- 97 2936 +/- 111 0.038614757 0.214412018 0.049592467 0.53631378 GPSN2 glycoprotein, synaptic 2 2089 +/- 57 2355 +/- 82 2194 +/- 58 2191 +/- 141 0.026365216 -0.982679638 0.011710413 0.562981612 GTF2H2 general transcription factor IIH, polypeptide 2, 44kDa 104 +/- 9 178 +/- 26 133 +/- 21 150 +/- 15 0.033469522 0.540628421 0.025370433 0.898946187 GUF1 GUF1 GTPase homolog (S. cerevisiae) 391 +/- 29 526 +/- 32 365 +/- 39 430 +/- 27 0.011668221 0.209153322 0.04373217 0.384947491 GUP1 GUP1 glycerol uptake/transporter homolog (S. cerevisiae) 602 +/- 64 1060 +/- 102 467 +/- 55 753 +/- 60 0.004636206 0.006825919 0.00498831 0.056779731 H6PD hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) 144 +/- 17 242 +/- 29 154 +/- 17 173 +/- 8 0.019765949 0.354234675 0.044519923 0.663460259 HEY2 hairy/enhancer-of-split related with YRPW motif 2 500 +/- 20 595 +/- 32 557 +/- 20 531 +/- 30 0.036020309 -0.480894388 0.043666703 0.361208249 HIGD1B HIG1 domain family, member 1B 225 +/- 13 268 +/- 11 154 +/- 8 203 +/- 12 0.030329094 0.009652683 0.032430077 0.057293297 HLA-DMA major histocompatibility complex, class II, DM alpha 100 +/- 12 213 +/- 26 81 +/- 17 132 +/- 17 0.005173868 0.062294997 0.006311469 0.137835884 HLA-DPA1 major histocompatibility complex, class II, DP alpha 1 242 +/- 37 469 +/- 34 224 +/- 35 351 +/- 66 0.001143936 0.139667186 0.023030694 0.322240505 HLA-DRA major histocompatibility complex, class II, DR alpha 537 +/- 59 893 +/- 114 350 +/- 59 668 +/- 140 0.025403664 0.086284694 0.028219491 0.302105457 HMG2L1 high-mobility group protein 2-like 1 827 +/- 48 1078 +/- 75 788 +/- 50 931 +/- 40 0.020869135 0.052828578 0.005334313 0.098226025 HMGN2 high-mobility group nucleosomal binding domain 2 197 +/- 23 265 +/- 10 221 +/- 5 255 +/- 33 0.031372344 0.37656245 0.022221567 0.690025154 HNRPH3 heterogeneous nuclear ribonucleoprotein H3 (2H9) 973 +/- 64 1240 +/- 83 1247 +/- 84 1322 +/- 104 0.030355051 0.590177248 0.025170851 0.929882825 HSMPP8 M-phase phosphoprotein, mpp8 1015 +/- 35 1210 +/- 68 1054 +/- 61 1091 +/- 69 0.035507417 0.696141378 0.007421117 0.44853766 ICAM1 intercellular adhesion molecule 1 (CD54), human rhinovirus receptor 120 +/- 10 185 +/- 13 126 +/- 9 167 +/- 8 0.002775713 0.007973606 0.00122463 0.186165299 IFIT3 interferon-induced protein with tetratricopeptide repeats 3 309 +/- 18 383 +/- 25 287 +/- 18 321 +/- 15 0.039785711 0.173070055 0.020436739 0.369955803 IFNAR1 interferon (alpha, beta and omega) receptor 1 514 +/- 18 825 +/- 71 497 +/- 23 630 +/- 59 0.006363447 0.088126266 0.001121053 0.130292473 IFNAR2 interferon (alpha, beta and omega) receptor 2 189 +/- 22 286 +/- 18 155 +/- 25 256 +/- 36 0.007188099 0.051828412 0.04034989 0.129988797 IGBP1 immunoglobulin (CD79A) binding protein 1 1965 +/- 47 2506 +/- 69 1885 +/- 71 1954 +/- 88 0.000131631 0.556127965 0.008860807 0.375876215 IGF1R Insulin-like growth factor 1 receptor 545 +/- 60 811 +/- 50 668 +/- 61 725 +/- 86 0.007162799 0.608118893 0.007735132 0.1993183 IGFBP5 insulin-like growth factor binding protein 5 174 +/- 19 323 +/- 54 358 +/- 138 309 +/- 76 0.039225587 -0.763077677 0.005648609 0.950195247 INADL InaD-like (Drosophila) 87 +/- 10 135 +/- 10 146 +/- 6 143 +/- 14 0.008422647 -0.845083805 0.007027185 0.499255628 ITM2A integral membrane protein 2A 1463 +/- 86 1990 +/- 49 1400 +/- 32 1806 +/- 148 0.000728073 0.050000797 0.020938239 0.247782417 LEAP-2 liver-expressed antimicrobial peptide 2 142 +/- 15 191 +/- 11 154 +/- 13 150 +/- 10 0.025160872 -0.775805855 0.008765374 0.545107181 LIFR leukemia inhibitory factor receptor alpha 2392 +/- 272 3256 +/- 164 2200 +/- 139 2374 +/- 110 0.025645983 0.350575343 0.018202869 0.811738741 LRRC8C Leucine rich repeat containing 8 family, member C 232 +/- 33 400 +/- 51 193 +/- 36 225 +/- 20 0.023303555 0.46499139 0.001155341 0.528368969 LTB lymphotoxin beta (TNF superfamily, member 3) 150 +/- 32 249 +/- 30 143 +/- 12 161 +/- 19 0.047103117 0.446585021 0.028083697 0.427868642 LTC4S leukotriene C4 synthase 236 +/- 25 314 +/- 22 201 +/- 17 191 +/- 16 0.041907623 -0.662253981 0.026573385 0.264391824 LYZ lysozyme (renal amyloidosis) 354 +/- 21 541 +/- 71 247 +/- 38 330 +/- 29 0.045574016 0.117092173 0.008001252 0.161253336

S4- MSI correlated and non-correlated ARGs.xls Page 6 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG MALAT1 metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA) 999 +/- 139 1887 +/- 224 1230 +/- 192 2019 +/- 444 0.009290459 0.158900801 0.015713805 0.240633665 MBD5 methyl-CpG binding domain protein 5 1291 +/- 137 1781 +/- 99 1091 +/- 72 1304 +/- 87 0.017422188 0.094875736 0.031445771 0.297900117 MBNL2 Muscleblind-like 2 (Drosophila) 431 +/- 36 550 +/- 27 566 +/- 22 603 +/- 44 0.027528449 0.492125993 0.002682924 0.063390729 MCCC1 methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) 588 +/- 18 799 +/- 27 614 +/- 25 820 +/- 25 0.000133926 0.00024902 0.025550241 0.064593462 MED6 mediator of RNA polymerase II transcription, subunit 6 homolog (S. cerevisiae) 256 +/- 21 344 +/- 26 281 +/- 19 322 +/- 15 0.025911598 0.12575465 0.014067235 0.100377475 MEGF10 multiple EGF-like-domains 10 775 +/- 88 1122 +/- 99 648 +/- 44 768 +/- 24 0.025981771 0.045695659 0.012992723 0.233386867 METTL7A methyltransferase like 7A 306 +/- 63 569 +/- 51 420 +/- 87 661 +/- 102 0.009059025 0.10798711 0.009236235 0.072246379 MKLN1 muskelin 1, intracellular mediator containing kelch motifs 727 +/- 42 901 +/- 60 770 +/- 27 780 +/- 19 0.040687805 0.759905764 0.024952674 0.99133024 MLLT10 Myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 10 261 +/- 16 374 +/- 37 406 +/- 32 426 +/- 73 0.027366183 0.811362091 0.008731269 0.412478243 MOSPD2 motile sperm domain containing 2 378 +/- 37 537 +/- 58 304 +/- 24 339 +/- 26 0.048005258 0.356207525 0.001365979 0.482852394 MYBBP1A MYB binding protein (P160) 1a 227 +/- 13 300 +/- 10 267 +/- 21 271 +/- 13 0.001540756 0.877026992 0.007627024 0.895285213 N4BP1 Nedd4 binding protein 1 375 +/- 24 511 +/- 28 401 +/- 42 438 +/- 25 0.004569425 0.473764695 0.004723022 0.35225073 NAG neuroblastoma-amplified protein 98 +/- 12 158 +/- 21 165 +/- 17 192 +/- 26 0.03855762 0.425888882 0.014356024 0.454265218 NAG8 nasopharyngeal carcinoma associated gene protein-8 312 +/- 11 399 +/- 15 334 +/- 33 363 +/- 26 0.001317776 0.517727444 0.005418344 0.419764977 NAP1L1 nucleosome assembly protein 1-like 1 1463 +/- 33 1829 +/- 95 1400 +/- 79 1475 +/- 74 0.010200022 0.499868406 0.00089931 0.25617108 NBR1 neighbor of BRCA1 gene 1 1100 +/- 31 1283 +/- 49 1205 +/- 34 1320 +/- 16 0.012247868 0.018008543 0.001584926 0.086231024 NEDD9 neural precursor cell expressed, developmentally down-regulated 9 171 +/- 24 264 +/- 31 142 +/- 23 201 +/- 20 0.043091499 0.088590079 0.047012718 0.040308284 NEU4 sialidase 4 318 +/- 28 531 +/- 71 374 +/- 25 540 +/- 33 0.029472306 0.003914097 0.002864159 0.068256338 NFIC nuclear factor I/C (CCAAT-binding transcription factor) 198 +/- 10 244 +/- 11 275 +/- 22 264 +/- 14 0.011185714 -0.689607333 0.004600124 0.894269355 NIPBL Nipped-B homolog (Drosophila) 651 +/- 32 814 +/- 41 781 +/- 26 815 +/- 32 0.011197479 0.434143199 0.006646694 0.638793267 NMNAT1 nicotinamide nucleotide adenylyltransferase 1 148 +/- 15 217 +/- 22 178 +/- 10 206 +/- 24 0.029165787 0.321266213 0.033943691 0.228857458 NPAT nuclear protein, ataxia-telangiectasia locus 490 +/- 15 619 +/- 32 435 +/- 15 535 +/- 41 0.007942717 0.071888741 0.022728098 0.114601362 NR3C1 Nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) 263 +/- 52 681 +/- 115 326 +/- 52 464 +/- 66 0.012776954 0.138402367 0.001005749 0.025478445 NRF1 nuclear respiratory factor 1 180 +/- 11 223 +/- 12 164 +/- 5 186 +/- 16 0.0251685 0.24626113 0.024937746 0.109653235 NUP50 nucleoporin 50kDa 2247 +/- 143 3427 +/- 362 2766 +/- 251 3193 +/- 321 0.020819898 0.325113982 0.0368692 0.442796014 NVL nuclear VCP-like 300 +/- 30 444 +/- 43 292 +/- 21 362 +/- 6 0.02316415 0.018625285 0.00138925 0.052693472 OLR1 oxidised low density lipoprotein (lectin-like) receptor 1 109 +/- 11 211 +/- 34 91 +/- 17 134 +/- 20 0.027477861 0.137990596 0.000134007 0.39771279 PABPC1 Poly(A) binding protein, cytoplasmic 1 183 +/- 21 254 +/- 18 240 +/- 24 272 +/- 26 0.028399619 0.400930331 0.011399941 0.662985494 PAK2 p21 (CDKN1A)-activated kinase 2 335 +/- 25 481 +/- 33 362 +/- 10 410 +/- 22 0.005919187 0.096546622 0.001697479 0.502297444 PALLD palladin, cytoskeletal associated protein 483 +/- 53 658 +/- 48 529 +/- 39 581 +/- 31 0.035167474 0.314433806 0.013614401 0.577514105 PARP3 poly (ADP-ribose) polymerase family, member 3 333 +/- 39 495 +/- 52 342 +/- 27 363 +/- 17 0.034081773 0.517761449 0.029808334 0.835411423 PCNP PEST proteolytic signal containing nuclear protein 4478 +/- 358 5960 +/- 334 4068 +/- 199 4741 +/- 246 0.012789221 0.065490967 0.006299784 0.229023219 PEA15 phosphoprotein enriched in astrocytes 15 14228 +/- 407 15719 +/- 171 16261 +/- 383 17626 +/- 635 0.012515894 0.110018067 0.01568957 0.349064488 PEX11A Peroxisomal biogenesis factor 11A 115 +/- 9 142 +/- 7 134 +/- 13 144 +/- 10 0.040807491 0.545280325 0.021457324 0.610457994 PFAAP5 phosphonoformate immuno-associated protein 5 540 +/- 18 663 +/- 31 644 +/- 46 682 +/- 23 0.009352546 0.484302786 0.036097245 0.999289381 PHF10 PHD finger protein 10 327 +/- 31 419 +/- 15 380 +/- 14 460 +/- 26 0.032516087 0.03490785 0.038848268 0.108541702 PHF21A PHD finger protein 21A 468 +/- 21 547 +/- 29 481 +/- 21 523 +/- 41 0.049419862 0.396797277 0.036087843 0.426568662 PHF3 PHD finger protein 3 1737 +/- 78 2049 +/- 109 1967 +/- 66 2013 +/- 122 0.044369987 0.752339308 0.001930597 0.231445138 PIGB phosphatidylinositol glycan, class B 477 +/- 19 647 +/- 44 496 +/- 15 519 +/- 26 0.009972818 0.456551497 0.001006657 0.558223664 PIK3C2A Phosphoinositide-3-kinase, class 2, alpha polypeptide 1130 +/- 44 1263 +/- 40 1085 +/- 33 1109 +/- 30 0.048115901 0.60382933 0.04856744 0.755961897 PLAGL2 pleiomorphic adenoma gene-like 2 189 +/- 12 240 +/- 12 225 +/- 13 207 +/- 23 0.013756967 -0.511906673 0.012805194 0.616393239 PLD2 phospholipase D2 201 +/- 20 312 +/- 13 231 +/- 27 280 +/- 18 0.001388392 0.165642833 0.00725353 0.202012138 PLEKHA1 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1 1885 +/- 104 2268 +/- 98 1949 +/- 90 1867 +/- 107 0.023450906 -0.575563954 0.047760243 0.849442366 PLXNA3 plexin A3 205 +/- 22 318 +/- 36 319 +/- 36 416 +/- 24 0.028119323 0.054836451 0.038607059 0.213522033

S4- MSI correlated and non-correlated ARGs.xls Page 7 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG PNN pinin, desmosome associated protein 870 +/- 34 1067 +/- 32 937 +/- 58 1028 +/- 61 0.001907097 0.307626496 0.038550779 0.843140151 PON2 paraoxonase 2 3263 +/- 296 4425 +/- 150 3317 +/- 229 3671 +/- 180 0.009092168 0.255642437 0.013117144 0.561779824 PPIA peptidylprolyl isomerase A (cyclophilin A) 281 +/- 45 463 +/- 32 349 +/- 57 440 +/- 15 0.00896267 0.174852469 0.015372548 0.261194992 PPP1R3E Protein phosphatase 1, regulatory (inhibitor) subunit 3E 694 +/- 57 902 +/- 71 992 +/- 47 1125 +/- 45 0.045732845 0.07117127 0.014881135 0.062308501 PPP4R2 protein phosphatase 4, regulatory subunit 2 886 +/- 44 1162 +/- 105 1013 +/- 60 1050 +/- 98 0.047172478 0.759742148 0.000298597 0.302120359 PPWD1 peptidylprolyl isomerase domain and WD repeat containing 1 248 +/- 10 294 +/- 14 268 +/- 8 252 +/- 15 0.022665211 -0.389624578 0.001342101 0.995511183 PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit 294 +/- 22 397 +/- 21 287 +/- 16 334 +/- 20 0.006689526 0.103805992 0.004126483 0.040563675 PSMA3 proteasome (prosome, macropain) subunit, alpha type, 3 1537 +/- 38 1871 +/- 67 1481 +/- 61 1913 +/- 144 0.002582344 0.03633931 0.047591605 0.162895805 PSMD11 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 1622 +/- 35 1788 +/- 38 1666 +/- 29 1742 +/- 29 0.009249267 0.094642681 0.005010925 0.225815001 PTPLAD2 protein tyrosine phosphatase-like A domain containing 2 114 +/- 22 191 +/- 25 74 +/- 9 99 +/- 11 0.041842202 0.112481777 0.023563093 0.431114428 RABGAP1 RAB GTPase activating protein 1 116 +/- 13 189 +/- 11 144 +/- 11 164 +/- 8 0.001623547 0.157810579 0.007450589 0.139733204 RANBP6 RAN binding protein 6 782 +/- 58 993 +/- 47 796 +/- 88 1061 +/- 36 0.018602372 0.028990382 0.04712257 0.074729428 RBBP6 retinoblastoma binding protein 6 272 +/- 14 345 +/- 18 397 +/- 27 330 +/- 14 0.010178181 -0.06165419 0.010117973 0.10574528 RBM25 RNA binding motif protein 25 1188 +/- 33 1453 +/- 33 1540 +/- 42 1830 +/- 101 0.000207634 0.041768993 0.005200005 0.226285734 REPS2 RALBP1 associated Eps domain containing 2 1087 +/- 40 1316 +/- 59 1227 +/- 99 1360 +/- 65 0.010674468 0.29518669 0.030018381 0.397531166 RFXANK regulatory factor X-associated ankyrin-containing protein 382 +/- 19 443 +/- 19 312 +/- 22 350 +/- 23 0.045587582 0.256114067 0.032492205 0.050362787 RGS12 regulator of G-protein signalling 12 350 +/- 21 452 +/- 21 478 +/- 32 437 +/- 24 0.006008656 -0.32488588 0.03311309 0.44511161 RNASE6 ribonuclease, RNase A family, k6 397 +/- 35 654 +/- 73 255 +/- 16 436 +/- 47 0.014909564 0.014505765 0.006242408 0.074681061 RP11-217H1.1 implantation-associated protein 540 +/- 57 800 +/- 92 433 +/- 35 574 +/- 40 0.041470768 0.027972795 0.00427697 0.14838873 RP13-297E16.1 DNA segment on chromosome X and Y (unique) 155 expressed sequence, isoform 1 610 +/- 39 823 +/- 49 675 +/- 31 664 +/- 44 0.00735769 -0.836698813 0.038927463 0.9081344 RPL23AP13 ribosomal protein L23a pseudogene 13 491 +/- 25 617 +/- 43 598 +/- 72 594 +/- 30 0.035949828 -0.961361435 0.024524444 0.903890038 RPL37 ribosomal protein L37 1311 +/- 97 1831 +/- 155 1601 +/- 107 1229 +/- 57 0.020800471 -0.016960897 0.031384133 0.072104543 RPL9 ribosomal protein L9 12030 +/- 543 14358 +/- 285 10191 +/- 563 9811 +/- 1825 0.006204199 -0.850373759 0.045490287 0.908068036 RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 1507 +/- 66 2010 +/- 89 1589 +/- 57 1912 +/- 83 0.001300869 0.013806977 0.043269804 0.121311674 RUFY3 RUN and FYVE domain containing 3 69 +/- 10 111 +/- 9 114 +/- 13 156 +/- 17 0.009915787 0.096063784 0.013841064 0.016151345 SAMD4A sterile alpha motif domain containing 4A 1424 +/- 134 2109 +/- 170 1803 +/- 166 1808 +/- 59 0.010800612 0.978024483 0.024403216 0.982968318 SAPS3 SAPS domain family, member 3 485 +/- 34 610 +/- 18 601 +/- 21 616 +/- 32 0.013313502 0.717097335 0.008074361 0.980177182 SCFD1 sec1 family domain containing 1 83 +/- 2 121 +/- 10 105 +/- 6 109 +/- 8 0.009437588 0.733604621 0.040812849 0.568056853 SCNM1 sodium channel modifier 1 257 +/- 18 318 +/- 18 261 +/- 8 288 +/- 10 0.036437885 0.059945743 0.030557839 0.272591936 SERPINA1 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 442 +/- 80 836 +/- 138 331 +/- 29 562 +/- 91 0.03926328 0.062066642 0.005075544 0.064324155 SF1 splicing factor 1 140 +/- 11 201 +/- 16 207 +/- 11 256 +/- 26 0.013710008 0.143511892 0.014611534 0.103229039 SFRS11 splicing factor, arginine/serine-rich 11 4017 +/- 267 5453 +/- 187 4660 +/- 175 5391 +/- 406 0.001713344 0.153882756 0.00023974 0.017460672 SFXN5 Sideroflexin 5 157 +/- 8 208 +/- 14 190 +/- 13 226 +/- 9 0.010970906 0.051919548 0.000275701 0.030631541 SGK serum/glucocorticoid regulated kinase 1969 +/- 150 2626 +/- 225 1220 +/- 179 1632 +/- 102 0.039052727 0.081610371 0.047104075 0.211883588 SH2B SH2-B homolog 1071 +/- 20 1347 +/- 59 1612 +/- 117 1439 +/- 90 0.004188871 -0.272412738 0.046181013 0.392791129 SH3PX3 SH3 and PX domain containing 3 243 +/- 24 325 +/- 26 209 +/- 22 227 +/- 14 0.044361279 0.515619215 0.046458236 0.793788545 SLA/LP soluble liver antigen/liver pancreas antigen 90 +/- 8 130 +/- 15 95 +/- 11 94 +/- 4 0.044906005 -0.958315905 0.034252707 0.907477351 SLC14A1 Solute carrier family 14 (urea transporter), member 1 (Kidd blood group) 669 +/- 53 1000 +/- 92 657 +/- 68 883 +/- 90 0.014166672 0.079572495 0.002606963 0.125666304 SLC30A5 solute carrier family 30 (zinc transporter), member 5 457 +/- 12 540 +/- 14 505 +/- 21 552 +/- 21 0.001169226 0.14546643 0.00418326 0.194415714 SMARCC1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 482 +/- 28 591 +/- 24 542 +/- 16 570 +/- 13 0.015250901 0.203948393 0.004373823 0.086363732 SMCHD1 structural maintenance of chromosomes flexible hinge domain containing 1 694 +/- 40 886 +/- 59 797 +/- 51 844 +/- 38 0.025249329 0.477217409 0.00017432 0.44487734 SMYD2 SET and MYND domain containing 2 109 +/- 17 181 +/- 16 144 +/- 19 192 +/- 26 0.011355268 0.180434029 0.003034957 0.015456498 SNRPG small nuclear ribonucleoprotein polypeptide G 658 +/- 21 808 +/- 50 678 +/- 26 740 +/- 42 0.027600557 0.25045861 0.000402187 0.100255428 SNX1 Sorting nexin 1 172 +/- 19 261 +/- 27 175 +/- 13 234 +/- 16 0.024268377 0.020264833 0.013460226 0.242657218

S4- MSI correlated and non-correlated ARGs.xls Page 8 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG SOS2 Son of sevenless homolog 2 (Drosophila) 298 +/- 20 354 +/- 13 311 +/- 25 351 +/- 34 0.039420881 0.369755871 0.032858812 0.445919076 SOX2 SRY (sex determining region Y)-box 2 4887 +/- 267 6273 +/- 249 4911 +/- 290 5382 +/- 228 0.003542595 0.233450594 0.009880218 0.65474299 SPAG9 sperm associated antigen 9 477 +/- 60 705 +/- 50 407 +/- 40 498 +/- 44 0.016127638 0.158938194 0.015124573 0.033519586 SRCAP Snf2-related CBP activator protein 190 +/- 10 242 +/- 8 232 +/- 5 203 +/- 9 0.002225566 -0.025473729 0.012583108 0.036958657 SS18 synovial sarcoma translocation, chromosome 18 179 +/- 34 427 +/- 43 209 +/- 34 419 +/- 49 0.00124615 0.00896963 0.00299713 0.062158137 ST3GAL6 ST3 beta-galactoside alpha-2,3-sialyltransferase 6 690 +/- 106 1161 +/- 162 768 +/- 42 966 +/- 63 0.039073465 0.033661876 0.012021888 0.074533991 SUHW3 suppressor of hairy wing homolog 3 (Drosophila) 164 +/- 8 195 +/- 7 232 +/- 8 239 +/- 11 0.012389407 0.66398156 0.001850589 0.414613197 SYNPO2L synaptopodin 2-like 274 +/- 17 355 +/- 22 261 +/- 23 265 +/- 16 0.016877881 0.895370348 0.025080952 0.85198709 TACC1 transforming, acidic coiled-coil containing protein 1 1582 +/- 114 2047 +/- 42 1759 +/- 107 2070 +/- 195 0.007784824 0.207245345 0.006509671 0.1016656 TAF1A TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa 204 +/- 13 258 +/- 14 251 +/- 18 253 +/- 11 0.017362486 0.923977769 0.002490684 0.910610537 TAF3 TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa 296 +/- 8 367 +/- 26 310 +/- 6 301 +/- 21 0.037240022 -0.710751417 0.00288843 0.317449677 TAS2R14 Taste receptor, type 2, member 14 101 +/- 20 203 +/- 19 104 +/- 21 230 +/- 27 0.004438424 0.005973521 0.036893291 0.098243184 TBL1X transducin (beta)-like 1X-linked 155 +/- 28 369 +/- 21 212 +/- 17 525 +/- 99 0.000145665 0.033161203 0.003346196 0.073986264 TCEAL8 transcription elongation factor A (SII)-like 8 2261 +/- 121 2724 +/- 87 2754 +/- 147 2815 +/- 38 0.012366046 0.698955524 0.014670865 0.606538238 TCF7L2 transcription factor 7-like 2 (T-cell specific, HMG-box) 1721 +/- 95 2204 +/- 120 1530 +/- 109 1712 +/- 113 0.010918545 0.276316647 0.000591916 0.481372973 TGOLN2 trans-golgi network protein 2 6539 +/- 384 7975 +/- 222 6466 +/- 326 7084 +/- 273 0.011924282 0.179939222 0.00286801 0.143496548 TJP2 tight junction protein 2 (zona occludens 2) 2291 +/- 204 3125 +/- 237 2144 +/- 139 2859 +/- 124 0.024080242 0.004053353 0.021048548 0.066068672 TLN2 Talin 2 152 +/- 19 223 +/- 17 218 +/- 33 262 +/- 39 0.019410687 0.416036769 0.016274768 0.130618477 TM7SF2 transmembrane 7 superfamily member 2 1226 +/- 65 1455 +/- 59 1141 +/- 34 1258 +/- 73 0.026139782 0.201389337 0.039240139 0.541288772 TM9SF3 transmembrane 9 superfamily member 3 965 +/- 31 1185 +/- 61 976 +/- 42 1008 +/- 62 0.013583593 0.68360539 3.79723E-05 0.224391729 TMCC1 Transmembrane and coiled-coil domain family 1 100 +/- 14 167 +/- 12 175 +/- 18 218 +/- 27 0.004556619 0.232235783 0.002972997 0.63077802 TMEM116 transmembrane protein 116 119 +/- 13 198 +/- 25 90 +/- 10 142 +/- 15 0.022720757 0.023265683 0.00913731 0.065412938 TMEM153 transmembrane protein 153 685 +/- 27 814 +/- 25 671 +/- 36 814 +/- 70 0.006062299 0.119131963 0.009375259 0.288559006 TMEM57 transmembrane protein 57 604 +/- 27 705 +/- 15 723 +/- 33 725 +/- 8 0.012641183 0.94554473 0.024223387 0.898087938 TncRNA trophoblast-derived noncoding RNA 1717 +/- 180 2482 +/- 183 1618 +/- 126 1986 +/- 125 0.013765549 0.068035047 0.003484289 0.025187375 TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 626 +/- 50 900 +/- 75 508 +/- 50 663 +/- 50 0.014442113 0.056884057 0.006526959 0.134142546 TOR1AIP1 torsin A interacting protein 1 848 +/- 70 1089 +/- 39 1211 +/- 42 1195 +/- 20 0.017623227 -0.739001031 0.016424322 0.791880907 TPM1 Tropomyosin 1 (alpha) 396 +/- 71 679 +/- 70 626 +/- 100 856 +/- 98 0.018042322 0.135835882 0.017410119 0.076079628 TTLL4 tubulin tyrosine ligase-like family, member 4 380 +/- 22 458 +/- 23 287 +/- 14 309 +/- 11 0.037352178 0.234890921 0.003115695 0.303117051 TUBGCP2 tubulin, gamma complex associated protein 2 1011 +/- 12 1088 +/- 28 991 +/- 31 1088 +/- 35 0.039627436 0.069900621 0.018753734 0.146028112 UBAP2L ubiquitin associated protein 2-like 1045 +/- 48 1221 +/- 34 950 +/- 19 1068 +/- 43 0.014530624 0.047736246 0.03302701 0.201222102 UBE2H ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 3587 +/- 143 4508 +/- 50 3408 +/- 125 4376 +/- 229 0.000799338 0.0091898 0.001633609 0.083514757 UBR2 ubiquitin protein ligase E3 component n-recognin 2 1287 +/- 52 1516 +/- 35 1269 +/- 37 1337 +/- 21 0.00568159 0.154153867 0.004776029 0.570131441 UFD1L ubiquitin fusion degradation 1 like (yeast) 1115 +/- 186 1832 +/- 170 1206 +/- 182 1617 +/- 52 0.017561515 0.073879423 0.024453269 0.133224714 UNC93B1 unc-93 homolog B1 (C. elegans) 102 +/- 18 164 +/- 21 91 +/- 9 122 +/- 14 0.049850783 0.105323297 0.026469171 0.089244382 VDP vesicle docking protein p115 333 +/- 23 487 +/- 24 387 +/- 27 440 +/- 22 0.000843677 0.162964835 0.000358763 0.068550449 VMO1 vitelline membrane outer layer 1 homolog (chicken) 691 +/- 50 1261 +/- 202 543 +/- 79 843 +/- 117 0.036084867 0.069831019 0.018085705 0.022592984 VPS13C Vacuolar protein sorting 13 homolog C (S. cerevisiae) 1099 +/- 47 1286 +/- 45 1139 +/- 97 1352 +/- 95 0.017536257 0.149297764 0.03060717 0.540462227 VPS39 vacuolar protein sorting 39 (yeast) 1307 +/- 34 1456 +/- 46 1449 +/- 64 1330 +/- 79 0.028284255 -0.273341707 0.031071472 0.549636271 VPS53 vacuolar protein sorting 53 (S. cerevisiae) 146 +/- 12 263 +/- 30 186 +/- 19 252 +/- 27 0.008952463 0.084191339 0.015665731 0.132000829 WAPAL wings apart-like homolog (Drosophila) 1297 +/- 39 1452 +/- 53 1324 +/- 21 1373 +/- 65 0.042275519 0.509868156 0.042660643 0.619148188 WBP1 WW domain binding protein 1 1823 +/- 116 2506 +/- 129 1839 +/- 149 2251 +/- 134 0.002859452 0.069745186 0.01038962 0.144705454 WDR20 WD repeat domain 20 126 +/- 15 179 +/- 17 107 +/- 16 129 +/- 10 0.039401975 0.281023215 0.005935076 0.415662845 WDR57 WD repeat domain 57 (U5 snRNP specific) 959 +/- 34 1105 +/- 11 958 +/- 17 1063 +/- 49 0.006368898 0.097724694 0.020008128 0.450139251

S4- MSI correlated and non-correlated ARGs.xls Page 9 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG WDR73 WD repeat domain 73 236 +/- 7 291 +/- 13 265 +/- 22 258 +/- 15 0.005607061 -0.775932378 0.012479651 0.687412444 WHDC1L1 WAS protein homology region 2 domain containing 1-like 1 103 +/- 9 161 +/- 16 133 +/- 14 116 +/- 11 0.014717258 -0.368476558 0.016603301 0.150887091 WNK1 WNK lysine deficient protein kinase 1 169 +/- 12 233 +/- 22 147 +/- 11 191 +/- 17 0.031108331 0.069392066 0.002053821 0.002962915 WTAP Wilms tumor 1 associated protein 519 +/- 31 710 +/- 21 683 +/- 41 771 +/- 66 0.000644678 0.292143156 0.000364712 0.043361186 WWP1 WW domain containing E3 ubiquitin protein ligase 1 1658 +/- 161 2181 +/- 165 1410 +/- 176 1562 +/- 24 0.046635749 0.43056962 0.026369665 0.358386347 YIF1B Yip1 interacting factor homolog B (S. cerevisiae) 521 +/- 34 657 +/- 43 514 +/- 32 631 +/- 45 0.031961627 0.067483699 0.012496965 0.00613698 YTHDC1 YTH domain containing 1 3734 +/- 134 4179 +/- 118 3676 +/- 128 4478 +/- 290 0.031495913 0.048116818 0.003623977 0.154046625 ZBTB26 zinc finger and BTB domain containing 26 380 +/- 28 505 +/- 16 502 +/- 29 427 +/- 37 0.004714113 -0.14891551 0.000943701 0.42790815 ZCCHC10 zinc finger, CCHC domain containing 10 274 +/- 13 341 +/- 16 327 +/- 16 328 +/- 20 0.00917033 0.953354453 0.026274897 0.593063344 ZCCHC6 zinc finger, CCHC domain containing 6 609 +/- 30 750 +/- 31 578 +/- 31 641 +/- 42 0.008699074 0.260144714 0.00859128 0.189160563 ZCCHC7 Zinc finger, CCHC domain containing 7 91 +/- 10 151 +/- 6 114 +/- 11 145 +/- 19 0.000998355 0.20520873 0.032460558 0.937846913 ZF HCF-binding transcription factor Zhangfei 481 +/- 22 590 +/- 26 608 +/- 20 692 +/- 20 0.0098783 0.016804892 0.007960292 0.350000711 ZNF134 zinc finger protein 134 (clone pHZ-15) 473 +/- 12 593 +/- 38 437 +/- 27 544 +/- 37 0.023399139 0.048350318 0.008654068 0.303351624 ZNF154 zinc finger protein 154 (pHZ-92) 164 +/- 17 242 +/- 25 176 +/- 18 248 +/- 31 0.028828358 0.08721744 0.000705013 0.014940208 ZNF347 zinc finger protein 347 126 +/- 11 178 +/- 7 162 +/- 16 181 +/- 15 0.004275722 0.409141591 0.016687606 0.832521387 ZNF416 zinc finger protein 416 101 +/- 8 139 +/- 8 105 +/- 12 116 +/- 13 0.007995464 0.556996454 0.016925074 0.558136122 ZNF435 zinc finger protein 435 110 +/- 9 147 +/- 5 143 +/- 12 160 +/- 5 0.009413535 0.248189252 0.004456147 0.717756639 ZNF501 zinc finger protein 501 137 +/- 4 217 +/- 10 206 +/- 8 206 +/- 8 0.000272779 -0.953660065 0.001635579 0.886399947 ZNF585A zinc finger protein 585A 327 +/- 14 417 +/- 21 339 +/- 13 322 +/- 21 0.00539514 -0.492896455 0.006654868 0.259685498 ZNF624 zinc finger protein 624 203 +/- 12 260 +/- 11 231 +/- 15 261 +/- 14 0.005365871 0.177365678 0.000662409 0.231093838 ZNF652 Zinc finger protein 652 479 +/- 32 629 +/- 35 465 +/- 24 532 +/- 28 0.01026129 0.106058264 0.032785032 0.271788859 ZNF669 zinc finger protein 669 417 +/- 25 647 +/- 62 504 +/- 23 634 +/- 44 0.011643971 0.038528957 0.017252972 0.059934437 ZNF70 Zinc finger protein 70 162 +/- 13 219 +/- 18 207 +/- 13 233 +/- 8 0.028252666 0.141060176 0.044734082 0.502014078 ZNF75 zinc finger protein 75 (D8C6) 118 +/- 9 171 +/- 11 232 +/- 23 191 +/- 11 0.003938271 -0.160313807 0.046445619 0.090135826 ZNFN1A1 zinc finger protein, subfamily 1A, 1 (Ikaros) 114 +/- 15 175 +/- 22 102 +/- 11 150 +/- 6 0.044945208 0.005622566 0.00837135 0.080888941 ZNFN1A5 zinc finger protein, subfamily 1A, 5 579 +/- 151 1239 +/- 161 624 +/- 101 993 +/- 136 0.013638527 0.061688737 0.007545414 0.01299765 Downregulated with Age and Correlated with MSI in CA Region ABHD14A abhydrolase domain containing 14A 1760 +/- 56 1515 +/- 91 1973 +/- 103 1818 +/- 29 -0.048823745 -0.200934909 0.037107144 0.326279233 ACP1 acid phosphatase 1, soluble 2644 +/- 165 2074 +/- 157 2357 +/- 91 2031 +/- 147 -0.031306264 -0.101820483 0.009087005 0.010682124 ACTR1A ARP1 actin-related protein 1 homolog A, centractin alpha (yeast) 3191 +/- 134 2571 +/- 136 2763 +/- 211 2529 +/- 81 -0.008683195 -0.339256057 0.001561701 0.593701263 ADD1 adducin 1 (alpha) 4262 +/- 135 3716 +/- 140 3921 +/- 116 3527 +/- 163 -0.018612912 -0.086088044 0.031312199 0.233714098 AKT3 V-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) 4120 +/- 100 3342 +/- 250 3311 +/- 184 3045 +/- 90 -0.025142948 -0.234271755 0.012196248 0.414665882 ANKRD34 ankyrin repeat domain 34 1681 +/- 72 1443 +/- 63 1781 +/- 88 1714 +/- 34 -0.031915353 -0.501221111 0.002543646 0.87556327 APRIN androgen-induced proliferation inhibitor 2075 +/- 34 1723 +/- 57 2125 +/- 61 1961 +/- 40 -0.000694951 -0.054459213 7.07854E-06 0.033862026 ARFIP2 ADP-ribosylation factor interacting protein 2 (arfaptin 2) 1246 +/- 34 1120 +/- 36 1244 +/- 43 1245 +/- 60 -0.029165003 0.986525832 0.00717194 0.512156151 ARHGEF7 Rho guanine nucleotide exchange factor (GEF) 7 3467 +/- 223 2866 +/- 102 2911 +/- 102 2790 +/- 61 -0.043673508 -0.336367816 0.022328275 0.410873 ARMC6 armadillo repeat containing 6 565 +/- 25 466 +/- 18 510 +/- 19 463 +/- 34 -0.009367028 -0.270281502 0.000970391 0.464418551 ARPC5L actin related protein 2/3 complex, subunit 5-like 2033 +/- 80 1711 +/- 88 1873 +/- 143 1847 +/- 133 -0.022095698 -0.897100227 0.010421642 0.878847962 ATP2C1 ATPase, Ca++ transporting, type 2C, member 1 2029 +/- 118 1514 +/- 111 1497 +/- 112 1332 +/- 42 -0.009806757 -0.213381487 0.004225894 0.246922906 ATP6V1D ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D 208 +/- 7 103 +/- 11 218 +/- 21 236 +/- 128 -0.000226418 0.891765597 0.00425807 0.65267287 ATP6V1H ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H 5128 +/- 416 3241 +/- 499 4550 +/- 162 3067 +/- 564 -0.016199222 -0.056256093 0.012431327 0.055570232 ATXN7L3 ataxin 7-like 3 4182 +/- 141 3742 +/- 124 3753 +/- 122 3589 +/- 83 -0.041159247 -0.297756867 0.005534061 0.775795267 B3GNTL1 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1 215 +/- 11 174 +/- 8 238 +/- 21 202 +/- 7 -0.011299386 -0.156849415 0.034322098 0.393748663 B4GALT5 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 2214 +/- 209 1568 +/- 99 1896 +/- 165 1568 +/- 81 -0.026375716 -0.117468677 0.021611884 0.166635882

S4- MSI correlated and non-correlated ARGs.xls Page 10 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG BAALC brain and acute leukemia, cytoplasmic 5677 +/- 195 4724 +/- 234 5418 +/- 180 4924 +/- 267 -0.011050277 -0.167427416 0.007071515 0.030302328 BZW1 basic leucine zipper and W2 domains 1 5216 +/- 116 4624 +/- 192 4100 +/- 120 3941 +/- 258 -0.028895928 -0.596853048 0.004740205 0.215058141 CACNB1 calcium channel, voltage-dependent, beta 1 subunit 1084 +/- 79 825 +/- 44 1214 +/- 70 1044 +/- 36 -0.021978762 -0.065895477 0.005600829 0.145536029 CAMSAP1 calmodulin regulated spectrin-associated protein 1 1864 +/- 55 1556 +/- 68 1653 +/- 70 1442 +/- 53 -0.005928848 -0.039506876 0.036371961 0.15892634 CAPNS1 calpain, small subunit 1 7708 +/- 486 6177 +/- 323 7211 +/- 399 6252 +/- 282 -0.028382546 -0.082826878 0.012855353 0.156197048 CASP6 Caspase 6, apoptosis-related cysteine peptidase 345 +/- 21 260 +/- 29 301 +/- 24 283 +/- 18 -0.040367944 -0.566645121 0.022999897 0.262617761 CCDC109A coiled-coil domain containing 109A 569 +/- 44 445 +/- 19 621 +/- 31 560 +/- 29 -0.036177615 -0.189660688 0.029781367 0.12063001 CCDC44 coiled-coil domain containing 44 792 +/- 36 669 +/- 27 578 +/- 22 598 +/- 31 -0.021618378 0.607390674 0.010653132 0.738039644 CCDC85A coiled-coil domain containing 85A 92 +/- 15 41 +/- 10 189 +/- 13 169 +/- 26 -0.02030043 -0.51673355 0.045054254 0.794482032 CCRN4L CCR4 carbon catabolite repression 4-like (S. cerevisiae) 357 +/- 52 222 +/- 9 332 +/- 36 323 +/- 35 -0.048060927 -0.855936884 0.035147379 0.618589201 CDK5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35) 3649 +/- 206 3034 +/- 85 4001 +/- 116 3504 +/- 169 -0.029701932 -0.04397449 0.015793702 0.307385394 CDKL1 Cyclin-dependent kinase-like 1 (CDC2-related kinase) 246 +/- 38 126 +/- 18 235 +/- 31 154 +/- 10 -0.024980063 -0.045552325 0.037404744 0.079403962 CDKN2D cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) 1595 +/- 115 1240 +/- 71 1433 +/- 53 1337 +/- 51 -0.029208008 -0.225029096 0.001639885 0.266898861 CDO1 cysteine dioxygenase, type I 3039 +/- 285 2226 +/- 153 2318 +/- 87 2399 +/- 90 -0.037330723 0.537164932 0.016448798 0.914334182 CKMT1B creatine kinase, mitochondrial 1B 6947 +/- 213 5592 +/- 354 5522 +/- 246 4526 +/- 212 -0.010827979 -0.013361634 0.013891007 0.127060756 CLUAP1 clusterin associated protein 1 364 +/- 28 271 +/- 19 359 +/- 25 394 +/- 15 -0.021404154 0.266992871 0.015051329 0.376842344 COL4A3 collagen, type IV, alpha 3 (Goodpasture antigen) 155 +/- 10 113 +/- 13 122 +/- 13 100 +/- 15 -0.027496734 -0.290670075 0.046867863 0.100008503 COPS6 COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) 4767 +/- 72 4463 +/- 89 4638 +/- 109 4477 +/- 61 -0.024833446 -0.232491253 0.004839491 0.337771603 COPS8 COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis) 3844 +/- 259 2904 +/- 248 3403 +/- 232 3169 +/- 263 -0.025701573 -0.521505534 0.003773273 0.195536642 CRI2 CREBBP/EP300 inhibitor 2 2677 +/- 69 2462 +/- 64 2961 +/- 148 2988 +/- 133 -0.045566133 0.896076572 0.013415481 0.95609094 CRYBA2 crystallin, beta A2 197 +/- 9 153 +/- 13 189 +/- 23 170 +/- 6 -0.022756633 -0.458025517 0.005340474 0.763202712 CRYZL1 crystallin, zeta (quinone reductase)-like 1 1205 +/- 66 921 +/- 78 933 +/- 90 851 +/- 78 -0.019966392 -0.505096205 0.004364084 0.458582837 CSNK1D casein kinase 1, delta 809 +/- 36 702 +/- 20 866 +/- 60 764 +/- 92 -0.031334104 -0.386763662 0.022109974 0.334433287 CTDP1 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1 433 +/- 12 380 +/- 8 395 +/- 17 398 +/- 24 -0.006233806 0.926064951 0.046369539 0.941872522 CYR61 cysteine-rich, angiogenic inducer, 61 179 +/- 15 130 +/- 13 126 +/- 20 128 +/- 12 -0.034656121 0.939443225 0.015025637 0.807506086 DAK dihydroxyacetone kinase 2 homolog (S. cerevisiae) 169 +/- 11 116 +/- 13 159 +/- 13 126 +/- 13 -0.009757921 -0.113635004 0.00752697 0.023707166 DCUN1D1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) 2306 +/- 41 1936 +/- 62 1971 +/- 52 1884 +/- 74 -0.000830228 -0.365085383 0.000397026 0.212589261 DENR density-regulated protein 231 +/- 38 119 +/- 19 238 +/- 27 245 +/- 52 -0.031447904 0.908312308 0.00898637 0.871063866 DHX16 DEAH (Asp-Glu-Ala-His) box polypeptide 16 670 +/- 35 550 +/- 12 568 +/- 33 500 +/- 24 -0.017229993 -0.127296515 0.011376473 0.303406537 DLG3 discs, large homolog 3 (neuroendocrine-dlg, Drosophila) 3182 +/- 238 2326 +/- 174 2460 +/- 135 2233 +/- 59 -0.017401287 -0.16781061 0.015618112 0.431419743 DMRTC1 DMRT-like family C1 490 +/- 23 258 +/- 25 324 +/- 18 327 +/- 38 -4.47826E-05 0.951773644 0.008628636 0.756719961 DNAJC7 DnaJ (Hsp40) homolog, subfamily C, member 7 2338 +/- 63 1862 +/- 122 2348 +/- 84 2059 +/- 107 -0.009345228 -0.065690886 0.034521808 0.381980453 DNAPTP6 DNA polymerase-transactivated protein 6 3467 +/- 109 3025 +/- 90 3104 +/- 157 3123 +/- 114 -0.011510724 0.92500822 0.001574129 0.641589374 DOK4 docking protein 4 476 +/- 29 384 +/- 21 433 +/- 40 391 +/- 20 -0.03121188 -0.376148114 0.000896279 0.414803325 DPF1 D4, zinc and double PHD fingers family 1 1631 +/- 91 1083 +/- 88 1997 +/- 111 1529 +/- 115 -0.001498473 -0.017396385 0.00842478 0.107797752 EEF1A2 eukaryotic translation elongation factor 1 alpha 2 12229 +/- 451 10754 +/- 445 11925 +/- 233 11098 +/- 267 -0.042273622 -0.046167272 0.014356403 0.381357782 EFNB2 ephrin-B2 1795 +/- 111 1406 +/- 118 910 +/- 113 742 +/- 59 -0.037150485 -0.223194193 0.046435826 0.363159643 ELF3 E74-like factor 3 (ets domain transcription factor, epithelial-specific ) 129 +/- 10 68 +/- 11 136 +/- 10 86 +/- 15 -0.002286897 -0.022561321 0.000951718 0.067420213 ELOVL4 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4 1955 +/- 115 1623 +/- 79 1858 +/- 101 1621 +/- 69 -0.042060417 -0.08690869 0.026610083 0.541673854 EXOSC5 exosome component 5 648 +/- 33 544 +/- 16 532 +/- 31 483 +/- 14 -0.025108962 -0.201459915 0.025174739 0.490893179 FADD Fas (TNFRSF6)-associated via death domain 417 +/- 17 322 +/- 14 411 +/- 35 337 +/- 8 -0.001535015 -0.090736256 0.036494757 0.320630908 FAT3 FAT tumor suppressor homolog 3 (Drosophila) 802 +/- 78 532 +/- 31 691 +/- 50 625 +/- 62 -0.016377377 -0.430341337 0.044512833 0.883702755 FBXO22 F-box protein 22 180 +/- 15 116 +/- 11 204 +/- 17 146 +/- 19 -0.007921782 -0.047840719 0.043884135 0.074214805 FEN1 flap structure-specific endonuclease 1 189 +/- 9 162 +/- 6 178 +/- 12 185 +/- 19 -0.033609122 0.754613555 0.006574422 0.716936333

S4- MSI correlated and non-correlated ARGs.xls Page 11 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG FHOD3 formin homology 2 domain containing 3 907 +/- 38 718 +/- 69 579 +/- 57 434 +/- 53 -0.043636352 -0.096170928 0.010336495 0.328247753 FKRP fukutin related protein 1114 +/- 40 971 +/- 30 1164 +/- 29 1050 +/- 21 -0.018582598 -0.010989279 0.037392418 0.111899405 FLJ35348 FLJ35348 1340 +/- 120 959 +/- 56 1239 +/- 60 1161 +/- 20 -0.023203876 -0.260508865 0.008914682 0.403420109 FLJ37035 FLJ37035 protein 344 +/- 33 221 +/- 20 323 +/- 33 207 +/- 33 -0.012535482 -0.035517647 0.041448292 0.106054095 FNDC5 fibronectin type III domain containing 5 519 +/- 27 422 +/- 25 470 +/- 26 433 +/- 42 -0.024752494 -0.48943447 0.030532703 0.24227571 FXYD7 FXYD domain containing ion transport regulator 7 3435 +/- 178 2940 +/- 109 4078 +/- 119 3624 +/- 147 -0.04407684 -0.042429162 0.00488822 0.483348228 GCHFR GTP cyclohydrolase I feedback regulator 417 +/- 27 263 +/- 11 403 +/- 32 315 +/- 18 -0.00123335 -0.044121673 0.003602917 0.26266741 GFM2 G elongation factor, mitochondrial 2 1138 +/- 34 958 +/- 37 1177 +/- 64 993 +/- 71 -0.005135292 -0.087733981 0.045076337 0.224179949 GGH gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) 1157 +/- 71 923 +/- 51 1159 +/- 47 1032 +/- 52 -0.024735773 -0.103653588 0.030684776 0.22737129 GPM6A glycoprotein M6A 20299 +/- 453 16530 +/- 744 18774 +/- 263 16875 +/- 764 -0.00234216 -0.066050248 0.024341915 0.325260136 GRINA glutamate receptor, ionotropic, N-methyl D-asparate-associated protein 1 (glutamate binding) 5549 +/- 298 4625 +/- 234 6955 +/- 300 6870 +/- 234 -0.036044273 -0.828972803 0.023231701 0.692819426 GRLF1 glucocorticoid receptor DNA binding factor 1 2246 +/- 57 1916 +/- 112 2476 +/- 123 2257 +/- 73 -0.031674663 -0.162091497 0.005926068 0.565902829 GRM5 Glutamate receptor, metabotropic 5 4099 +/- 219 3135 +/- 171 3523 +/- 175 2644 +/- 98 -0.006510561 -0.002590439 0.006481496 0.085829727 GRTP1 Growth hormone regulated TBC protein 1 446 +/- 50 299 +/- 12 412 +/- 52 272 +/- 16 -0.030152815 -0.042958386 0.035210678 0.103772623 GSK3A glycogen synthase kinase 3 alpha 4199 +/- 213 3357 +/- 277 4155 +/- 242 3593 +/- 133 -0.038138935 -0.078496021 0.016454623 0.048104055 HEXIM1 hexamethylene bis-acetamide inducible 1 1258 +/- 39 1102 +/- 16 1185 +/- 37 1098 +/- 42 -0.008471309 -0.15592394 0.018829727 0.494076237 HIGD2A HIG1 domain family, member 2A 3641 +/- 182 2920 +/- 183 3456 +/- 163 2935 +/- 93 -0.019088664 -0.024690915 0.023971733 0.087432337 HLF hepatic leukemia factor 3087 +/- 125 2470 +/- 174 3295 +/- 234 3001 +/- 197 -0.017992173 -0.361245248 0.021444218 0.56515568 HMBOX1 homeobox containing 1 2019 +/- 129 1029 +/- 14 1858 +/- 133 1219 +/- 270 -0.000547214 -0.078476466 0.002248502 0.011675758 HNMT histamine N-methyltransferase 396 +/- 32 235 +/- 22 494 +/- 59 338 +/- 17 -0.002524332 -0.044935424 0.021349226 0.072377414 HOMER1 homer homolog 1 (Drosophila) 2901 +/- 169 2385 +/- 132 2363 +/- 91 2257 +/- 80 -0.038188312 -0.408436278 0.044859532 0.302280397 HP1BP3 heterochromatin protein 1, binding protein 3 5237 +/- 233 4163 +/- 95 5620 +/- 224 4368 +/- 255 -0.004208435 -0.005506694 0.030837236 0.073044113 IDH3B isocitrate dehydrogenase 3 (NAD+) beta 1428 +/- 48 1204 +/- 29 1103 +/- 38 1036 +/- 36 -0.003797664 -0.233414228 0.028620692 0.701024795 ITFG1 Integrin alpha FG-GAP repeat containing 1 7600 +/- 215 6251 +/- 221 6538 +/- 483 6093 +/- 241 -0.001403403 -0.437198048 0.008395414 0.622297603 ITIH5 inter-alpha (globulin) inhibitor H5 396 +/- 40 251 +/- 36 310 +/- 50 237 +/- 35 -0.023919047 -0.263543278 0.01618079 0.156373512 JMJD4 jumonji domain containing 4 451 +/- 27 321 +/- 25 448 +/- 15 402 +/- 26 -0.005768285 -0.172553026 0.011751809 0.3260716 KARCA1 kelch/ankyrin repeat containing cyclin A1 interacting protein 2299 +/- 44 1908 +/- 148 2213 +/- 74 1909 +/- 113 -0.045071747 -0.058893396 0.013447787 0.013397673 KCTD17 potassium channel tetramerisation domain containing 17 288 +/- 31 189 +/- 16 389 +/- 31 381 +/- 41 -0.022213908 -0.885017111 0.024220279 0.706816049 KIF3C kinesin family member 3C 5625 +/- 458 4054 +/- 161 4195 +/- 251 3767 +/- 99 -0.01695736 -0.160419755 0.005118117 0.230233843 KPNA1 karyopherin alpha 1 (importin alpha 5) 2311 +/- 102 1982 +/- 79 2132 +/- 68 2092 +/- 86 -0.030322121 -0.725154086 0.042620928 0.972189568 LARP1 La ribonucleoprotein domain family, member 1 2654 +/- 61 2210 +/- 101 2989 +/- 126 2762 +/- 90 -0.005348324 -0.178466454 0.004417523 0.176511386 LASS5 LAG1 longevity assurance homolog 5 (S. cerevisiae) 1328 +/- 43 993 +/- 51 1277 +/- 72 1037 +/- 33 -0.000565873 -0.01899967 0.000457208 0.127403657 LASS6 LAG1 longevity assurance homolog 6 (S. cerevisiae) 241 +/- 14 185 +/- 9 233 +/- 20 237 +/- 21 -0.009062972 0.911094789 0.001828051 0.699286235 LNX1 ligand of numb-protein X 1 2480 +/- 118 1905 +/- 184 2474 +/- 174 1901 +/- 108 -0.028569789 -0.022991433 0.002603438 0.156600864 LRPPRC leucine-rich PPR-motif containing 4632 +/- 236 3748 +/- 142 4099 +/- 157 3800 +/- 34 -0.011933882 -0.116117512 0.013012111 0.273372239 LRRFIP1 leucine rich repeat (in FLII) interacting protein 1 1529 +/- 101 1224 +/- 53 1604 +/- 56 1461 +/- 75 -0.029900488 -0.167583151 0.045979168 0.224863951 LRRN5 leucine rich repeat neuronal 5 1461 +/- 68 1120 +/- 25 1554 +/- 45 1289 +/- 57 -0.002903974 -0.006256578 0.007370126 0.064886225 LZTR2 Leucine zipper transcription regulator 2 124 +/- 12 87 +/- 9 134 +/- 23 117 +/- 16 -0.033318797 -0.554796942 0.003210529 0.144445843 MANEAL mannosidase, endo-alpha-like 2351 +/- 85 2004 +/- 64 2117 +/- 72 1985 +/- 75 -0.009303669 -0.239499435 0.004388795 0.125033905 MAP6 microtubule-associated protein 6 1084 +/- 21 874 +/- 23 1109 +/- 50 972 +/- 50 -4.96E-05 -0.084487817 0.003532582 0.320593099 MAPK8IP1 mitogen-activated protein kinase 8 interacting protein 1 4276 +/- 133 3711 +/- 134 3607 +/- 165 3762 +/- 73 -0.013644007 0.420129141 0.026394892 0.342453848 MAPK9 Mitogen-activated protein kinase 9 4733 +/- 223 3991 +/- 177 4745 +/- 208 4279 +/- 155 -0.02708885 -0.107047938 0.004574689 0.528747969 MAPRE3 microtubule-associated protein, RP/EB family, member 3 3330 +/- 90 2945 +/- 89 3841 +/- 102 3654 +/- 41 -0.012243103 -0.134441689 0.024023541 0.333593529 MATR3 matrin 3 18535 +/- 672 15789 +/- 403 18512 +/- 661 16674 +/- 807 -0.007753637 -0.115262499 0.03479029 0.212920471

S4- MSI correlated and non-correlated ARGs.xls Page 12 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG MCF2L MCF.2 cell line derived transforming sequence-like 169 +/- 11 113 +/- 10 232 +/- 24 135 +/- 8 -0.00333276 -0.007613212 0.033674994 0.067935138 MFAP1 microfibrillar-associated protein 1 1476 +/- 22 1315 +/- 35 1367 +/- 41 1252 +/- 27 -0.004145342 -0.047851898 0.002324551 0.062969353 M-RIP myosin phosphatase-Rho interacting protein 3617 +/- 157 3133 +/- 138 3127 +/- 109 2889 +/- 119 -0.043121247 -0.177578797 0.001489897 0.356422824 MRP63 mitochondrial ribosomal protein 63 1794 +/- 66 1575 +/- 30 1880 +/- 76 1746 +/- 80 -0.019640034 -0.25759186 0.042154311 0.304660353 MTG1 mitochondrial GTPase 1 homolog (S. cerevisiae) 1704 +/- 61 1474 +/- 43 1403 +/- 55 1356 +/- 42 -0.012619947 -0.518009595 0.026703714 0.549806975 MXRA7 matrix-remodelling associated 7 203 +/- 23 119 +/- 18 218 +/- 27 164 +/- 25 -0.018306801 -0.174309915 0.047168645 0.216235093 MYADM myeloid-associated differentiation marker 444 +/- 27 337 +/- 28 548 +/- 77 393 +/- 23 -0.020701463 -0.104459613 0.019736608 0.107923516 NAT5 N-acetyltransferase 5 5238 +/- 136 4713 +/- 92 5091 +/- 104 4770 +/- 113 -0.011363897 -0.067794632 0.021574602 0.271896906 NDUFA1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa 12545 +/- 378 10794 +/- 469 11641 +/- 107 11222 +/- 234 -0.016287758 -0.157323197 0.003711155 0.013642841 NDUFB10 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa 397 +/- 30 271 +/- 22 317 +/- 19 301 +/- 26 -0.007124239 -0.622555222 0.00410179 0.242804193 NMT2 N-myristoyltransferase 2 443 +/- 35 350 +/- 17 594 +/- 32 534 +/- 28 -0.045268927 -0.185806508 0.034401806 0.203357658 NPAS2 neuronal PAS domain protein 2 1237 +/- 69 1027 +/- 36 1162 +/- 50 1055 +/- 32 -0.028195974 -0.104973437 0.005994627 0.381351419 NRXN1 neurexin 1 10862 +/- 245 9499 +/- 402 10764 +/- 142 9886 +/- 287 -0.019307751 -0.033930344 0.031491478 0.148727641 NUBP1 nucleotide binding protein 1 (MinD homolog, E. coli) 679 +/- 25 548 +/- 12 607 +/- 21 506 +/- 19 -0.002200702 -0.006861505 0.025427377 0.077320833 NUDT10 nudix (nucleoside diphosphate linked moiety X)-type motif 10 282 +/- 18 221 +/- 15 266 +/- 20 231 +/- 22 -0.025399755 -0.26581319 0.005550744 0.16394202 NUTF2 Nuclear transport factor 2 611 +/- 38 479 +/- 23 607 +/- 22 528 +/- 32 -0.017070133 -0.081000563 0.022600983 0.138572292 OCRL oculocerebrorenal syndrome of Lowe 5167 +/- 351 2651 +/- 414 4835 +/- 441 3145 +/- 483 -0.000997411 -0.030467437 0.028945575 0.277285789 ODZ4 odz, odd Oz/ten-m homolog 4 (Drosophila) 1909 +/- 94 1463 +/- 108 1647 +/- 86 1434 +/- 117 -0.011218559 -0.179929322 0.004251082 0.064251086 OGDH oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1659 +/- 60 1455 +/- 67 1434 +/- 34 1400 +/- 37 -0.045988285 -0.523984078 0.040444081 0.383951287 PAPOLG poly(A) polymerase gamma 791 +/- 23 698 +/- 22 821 +/- 24 839 +/- 22 -0.014572723 0.597447164 0.041461965 0.852354691 PCBP1 poly(rC) binding protein 1 3493 +/- 69 3140 +/- 89 3412 +/- 103 3322 +/- 114 -0.011417071 -0.572878733 0.007884979 0.49870789 PCSK2 proprotein convertase subtilisin/kexin type 2 3146 +/- 220 2328 +/- 198 2601 +/- 119 2049 +/- 105 -0.020417862 -0.006951289 0.01232449 0.060491027 PFDN1 prefoldin subunit 1 3757 +/- 69 3495 +/- 74 3517 +/- 72 3281 +/- 42 -0.026757747 -0.0223749 0.003461971 0.103413488 PHLDA1 pleckstrin homology-like domain, family A, member 1 421 +/- 36 319 +/- 24 453 +/- 52 320 +/- 63 -0.043072063 -0.140808341 0.014174988 0.121035617 PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta) 5364 +/- 460 3833 +/- 348 4458 +/- 93 3892 +/- 156 -0.025607679 -0.017781087 0.021090149 0.095284858 PINK1 PTEN induced putative kinase 1 5970 +/- 290 3813 +/- 313 5732 +/- 418 4288 +/- 150 -0.000503668 -0.016543541 0.005077902 0.11399275 PIP5K1B phosphatidylinositol-4-phosphate 5-kinase, type I, beta 1195 +/- 134 787 +/- 70 608 +/- 59 541 +/- 13 -0.028872976 -0.314437372 0.018183945 0.262335204 PLA2G12A phospholipase A2, group XIIA 659 +/- 57 425 +/- 65 583 +/- 43 397 +/- 74 -0.021906837 -0.068932929 0.004271189 0.005247377 PLAA phospholipase A2-activating protein 952 +/- 29 788 +/- 29 921 +/- 43 883 +/- 20 -0.002376943 -0.443566093 0.010247002 0.871137589 PLCXD2 Phosphatidylinositol-specific phospholipase C, X domain containing 2 4569 +/- 505 3022 +/- 283 4069 +/- 225 3379 +/- 431 -0.028738374 -0.204459712 0.025954362 0.405116264 PNMA1 paraneoplastic antigen MA1 10959 +/- 391 9451 +/- 261 10858 +/- 318 10579 +/- 360 -0.011210692 -0.577070644 0.005618811 0.487260923 POLDIP2 polymerase (DNA-directed), delta interacting protein 2 1060 +/- 90 803 +/- 50 1016 +/- 25 1023 +/- 36 -0.039050768 0.872959524 0.018963059 0.637065539 POLE4 polymerase (DNA-directed), epsilon 4 (p12 subunit) 2611 +/- 103 2205 +/- 69 2073 +/- 57 2027 +/- 37 -0.009910754 -0.524049886 0.003033511 0.945593021 PPID peptidylprolyl isomerase D (cyclophilin D) 303 +/- 34 207 +/- 13 325 +/- 26 339 +/- 63 -0.03497557 0.847745854 0.031541523 0.278841929 PRDX5 peroxiredoxin 5 3941 +/- 95 3412 +/- 66 3326 +/- 71 3314 +/- 37 -0.00139971 -0.885483636 0.002094863 0.917583283 PRNPIP prion protein interacting protein 2458 +/- 123 2089 +/- 74 2388 +/- 39 2292 +/- 67 -0.032218204 -0.260049619 0.026413434 0.103059674 PROS1 protein S (alpha) 774 +/- 108 426 +/- 28 473 +/- 63 375 +/- 23 -0.022169032 -0.190453 0.033540833 0.246244928 PRPS2 phosphoribosyl pyrophosphate synthetase 2 1966 +/- 106 1443 +/- 29 1604 +/- 58 1369 +/- 46 -0.003525035 -0.011978454 0.001513869 0.084104019 PRSS22 protease, serine, 22 376 +/- 33 245 +/- 15 364 +/- 29 215 +/- 21 -0.009083568 -0.002358389 0.046989389 0.059613239 PSME3 proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) 1539 +/- 66 1271 +/- 42 1431 +/- 65 1338 +/- 73 -0.008494401 -0.364470977 0.003356471 0.415658339 PTHB1 parathyroid hormone-responsive B1 158 +/- 16 111 +/- 9 140 +/- 6 113 +/- 12 -0.03363722 -0.089110273 0.030504403 0.015137249 PTPRN2 protein tyrosine phosphatase, receptor type, N polypeptide 2 6494 +/- 194 5488 +/- 322 6194 +/- 400 5533 +/- 188 -0.027431781 -0.178053105 0.00140769 0.31327238 RAB6B RAB6B, member RAS oncogene family 1509 +/- 149 954 +/- 120 1616 +/- 206 1444 +/- 168 -0.016509018 -0.532763127 0.011962204 0.524906192 RANGNRF RAN guanine nucleotide release factor 425 +/- 34 318 +/- 15 318 +/- 17 272 +/- 27 -0.024130804 -0.192118213 0.015289187 0.020533647

S4- MSI correlated and non-correlated ARGs.xls Page 13 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG RASGRF2 Ras protein-specific guanine nucleotide-releasing factor 2 335 +/- 22 273 +/- 17 423 +/- 53 365 +/- 12 -0.048142485 -0.333608722 0.032839332 0.353359145 RBM9 RNA binding motif protein 9 5783 +/- 65 4948 +/- 171 5058 +/- 152 4836 +/- 215 -0.003138547 -0.424617309 0.002847488 0.624653344 RBMS1 RNA binding motif, single stranded interacting protein 1 195 +/- 15 138 +/- 12 149 +/- 8 122 +/- 10 -0.016158259 -0.05922107 0.041609338 0.283416898 REEP1 receptor accessory protein 1 3525 +/- 94 2995 +/- 201 2782 +/- 183 2618 +/- 100 -0.048147325 -0.455031497 0.01331036 0.861296448 REXO1 REX1, RNA exonuclease 1 homolog (S. cerevisiae) 655 +/- 28 517 +/- 30 915 +/- 58 744 +/- 67 -0.007208564 -0.087530236 0.001985453 0.243132623 RHEB Ras homolog enriched in brain 7133 +/- 176 6424 +/- 174 6526 +/- 211 6223 +/- 272 -0.016740681 -0.404730199 0.001094097 0.224940857 RHOBTB2 Rho-related BTB domain containing 2 686 +/- 32 533 +/- 29 663 +/- 45 502 +/- 41 -0.005244694 -0.028313851 0.007948868 0.08870149 RNF165 ring finger protein 165 582 +/- 25 444 +/- 19 525 +/- 34 419 +/- 29 -0.001550293 -0.042494447 0.043446064 0.191880592 RNF180 ring finger protein 180 252 +/- 11 196 +/- 5 237 +/- 14 213 +/- 19 -0.00212079 -0.336433705 0.010142879 0.258338931 ROBO2 roundabout, axon guidance receptor, homolog 2 (Drosophila) 1844 +/- 48 1630 +/- 69 1649 +/- 73 1537 +/- 82 -0.032174321 -0.337925299 0.001025989 0.646160903 RPP30 ribonuclease P/MRP 30kDa subunit 391 +/- 15 345 +/- 14 392 +/- 26 360 +/- 9 -0.044503625 -0.284057221 0.014671955 0.422890934 RPP40 ribonuclease P 40kDa subunit 814 +/- 28 680 +/- 26 723 +/- 28 663 +/- 58 -0.006008724 -0.38914678 0.001062214 0.352899497 RTF1 Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) 1080 +/- 61 889 +/- 52 1157 +/- 39 1177 +/- 28 -0.039211532 0.687223527 0.016679497 0.988203171 RUNX1T1 runt-related transcription factor 1; translocated to, 1 (cyclin D-related) 2489 +/- 125 1740 +/- 89 2274 +/- 171 1707 +/- 184 -0.000846719 -0.051017315 0.01645597 0.495650684 SAP130 Sin3A-associated protein, 130kDa 1623 +/- 42 1504 +/- 24 1469 +/- 39 1472 +/- 40 -0.039160451 0.964552301 0.023218694 0.486987024 SDCCAG1 serologically defined colon cancer antigen 1 388 +/- 20 315 +/- 15 516 +/- 63 441 +/- 28 -0.017002505 -0.314804602 0.003703351 0.196201543 SEC22C SEC22 vesicle trafficking protein homolog C (S. cerevisiae) 250 +/- 23 183 +/- 15 231 +/- 16 204 +/- 13 -0.038380002 -0.216114251 0.024485624 0.128538513 SEPT3 septin 3 7220 +/- 468 5490 +/- 263 8056 +/- 320 7357 +/- 258 -0.01245062 -0.124099563 0.021360115 0.456052172 SET SET translocation (myeloid leukemia-associated) 698 +/- 22 583 +/- 14 1015 +/- 52 866 +/- 35 -0.002192832 -0.042420255 0.020561031 0.052270741 SEZ6L2 Seizure related 6 homolog (mouse)-like 2 1565 +/- 18 1244 +/- 115 2230 +/- 72 2186 +/- 114 -0.038027127 -0.756043538 0.043918098 0.930399218 SFRS14 splicing factor, arginine/serine-rich 14 2501 +/- 104 2096 +/- 137 2785 +/- 51 2631 +/- 85 -0.041620035 -0.167002444 0.006250063 0.035349435 SFXN1 sideroflexin 1 947 +/- 84 649 +/- 46 800 +/- 33 630 +/- 29 -0.014753663 -0.004024948 0.022844764 0.055998664 SHANK2 SH3 and multiple ankyrin repeat domains 2 3124 +/- 137 2183 +/- 63 2942 +/- 130 2120 +/- 154 -0.000417935 -0.003276605 0.000954574 0.074945144 SHMT2 serine hydroxymethyltransferase 2 (mitochondrial) 193 +/- 15 139 +/- 8 177 +/- 12 147 +/- 8 -0.014906527 -0.071431424 0.031662802 0.10989783 SLC12A5 solute carrier family 12, (potassium-chloride transporter) member 5 9260 +/- 395 7923 +/- 381 8510 +/- 287 7677 +/- 214 -0.035165521 -0.045788367 0.015477601 0.202189808 SLC31A1 solute carrier family 31 (copper transporters), member 1 325 +/- 19 228 +/- 24 334 +/- 28 277 +/- 30 -0.011782491 -0.198780723 0.033509963 0.56732771 SLC35A2 solute carrier family 35 (UDP-galactose transporter), member A2 628 +/- 18 538 +/- 17 657 +/- 30 593 +/- 23 -0.004801971 -0.127267395 0.015822656 0.292141458 SLC39A3 solute carrier family 39 (zinc transporter), member 3 529 +/- 35 429 +/- 22 453 +/- 33 436 +/- 14 -0.039489178 -0.651066071 0.007249755 0.505237474 SOX11 SRY (sex determining region Y)-box 11 664 +/- 52 459 +/- 46 303 +/- 32 221 +/- 13 -0.014598477 -0.04875412 0.011762268 0.055596693 SPTY2D1 SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) 213 +/- 23 140 +/- 13 201 +/- 16 189 +/- 22 -0.025218651 -0.667917187 0.045809532 0.230201875 SSR2 signal sequence receptor, beta (translocon-associated protein beta) 2068 +/- 137 1585 +/- 78 1623 +/- 93 1483 +/- 68 -0.015559833 -0.256528135 0.004808131 0.149857836 SSTR2 somatostatin receptor 2 181 +/- 26 94 +/- 7 135 +/- 18 66 +/- 7 -0.018011504 -0.010196622 0.005480342 0.055425313 ST6GALNAC6 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferas 422 +/- 12 305 +/- 23 413 +/- 44 385 +/- 11 -0.002102437 -0.562836909 0.009006579 0.865391561 ST8SIA2 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 339 +/- 35 212 +/- 17 124 +/- 23 93 +/- 11 -0.013714468 -0.261208901 0.036940154 0.608622387 STMN2 stathmin-like 2 15575 +/- 785 11635 +/- 678 13350 +/- 985 11673 +/- 910 -0.003625593 -0.242693872 0.012395585 0.520895886 STS steroid sulfatase (microsomal), arylsulfatase C, isozyme S 522 +/- 29 428 +/- 25 424 +/- 42 346 +/- 14 -0.033390554 -0.12459381 0.02676154 0.18526026 SUGT1 SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) 266 +/- 18 181 +/- 12 342 +/- 23 283 +/- 32 -0.003216278 -0.181554741 0.032597684 0.739131261 SYT11 synaptotagmin XI 2979 +/- 924 523 +/- 91 2747 +/- 804 738 +/- 96 -0.044900169 -0.05440499 0.045545221 0.091856384 TAB3 TAK1-binding protein 3 1451 +/- 63 1273 +/- 30 1302 +/- 27 1272 +/- 19 -0.036764609 -0.391401901 0.027287499 0.371004476 TADA3L transcriptional adaptor 3 (NGG1 homolog, yeast)-like 1631 +/- 31 1444 +/- 60 1480 +/- 44 1513 +/- 52 -0.02541244 0.647547172 0.031751945 0.562861226 TBC1D16 TBC1 domain family, member 16 132 +/- 15 84 +/- 12 134 +/- 13 147 +/- 12 -0.030784925 0.494537781 0.01661304 0.594546892 TBC1D22B TBC1 domain family, member 22B 1294 +/- 39 1120 +/- 36 1174 +/- 53 1149 +/- 18 -0.008401332 -0.662916412 0.004056769 0.872119536 TBC1D9 TBC1 domain family, member 9 5875 +/- 79 5392 +/- 40 4902 +/- 118 5160 +/- 124 -0.000792803 0.165631713 0.000688738 0.601842809 TBL3 transducin (beta)-like 3 579 +/- 36 464 +/- 23 541 +/- 36 472 +/- 24 -0.024171196 -0.141556092 0.017076025 0.209704541

S4- MSI correlated and non-correlated ARGs.xls Page 14 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG TEKT2 tektin 2 (testicular) 171 +/- 8 125 +/- 7 156 +/- 5 133 +/- 14 -0.001606702 -0.191238704 0.029531003 0.670140761 TERF2 telomeric repeat binding factor 2 123 +/- 13 83 +/- 8 103 +/- 11 90 +/- 11 -0.027877746 -0.404463565 0.002010455 0.418601379 TEX10 testis expressed sequence 10 462 +/- 12 415 +/- 8 455 +/- 18 446 +/- 12 -0.010266707 -0.664460157 0.019684954 0.765926009 TFG TRK-fused gene 5280 +/- 199 4443 +/- 99 4815 +/- 94 4186 +/- 147 -0.006471633 -0.008782778 0.008840003 0.243183596 THAP11 THAP domain containing 11 1105 +/- 29 934 +/- 18 1207 +/- 52 1091 +/- 55 -0.000838358 -0.16246445 0.018322576 0.064928883 TICAM1 toll-like receptor adaptor molecule 1 274 +/- 11 229 +/- 13 241 +/- 16 230 +/- 10 -0.025244948 -0.555689792 0.003575588 0.433435891 TIMM10 translocase of inner mitochondrial membrane 10 homolog (yeast) 1629 +/- 120 1047 +/- 77 1208 +/- 47 967 +/- 68 -0.003153302 -0.021238258 0.00548846 0.144075924 TIMM17B translocase of inner mitochondrial membrane 17 homolog B (yeast) 797 +/- 21 693 +/- 17 734 +/- 44 746 +/- 42 -0.003377767 0.851200083 0.001345578 0.862495685 TMEM14B transmembrane protein 14B 10702 +/- 355 8858 +/- 414 10426 +/- 173 9258 +/- 433 -0.007201764 -0.051728584 0.000494027 0.001648848 TMEM16D transmembrane protein 16D 287 +/- 24 220 +/- 14 135 +/- 14 141 +/- 14 -0.043746824 0.7783487 0.02635436 0.880005268 TMEM30A transmembrane protein 30A 8163 +/- 210 7246 +/- 252 7449 +/- 446 7041 +/- 398 -0.019401441 -0.51194934 0.028444652 0.921630753 TMEM76 transmembrane protein 76 3761 +/- 96 3217 +/- 173 4452 +/- 182 4256 +/- 291 -0.025905248 -0.587721374 0.021569628 0.343968639 TMEM91 transmembrane protein 91 837 +/- 63 517 +/- 43 501 +/- 26 438 +/- 36 -0.002489871 -0.196616967 0.010463406 0.547819959 TNRC4 trinucleotide repeat containing 4 4534 +/- 210 3677 +/- 260 5558 +/- 185 4760 +/- 252 -0.029148199 -0.035166821 0.010782622 0.125680243 TOLLIP toll interacting protein 3373 +/- 197 2715 +/- 128 3379 +/- 182 2936 +/- 92 -0.021607732 -0.065071858 0.007420979 0.124538741 TOMM40L translocase of outer mitochondrial membrane 40 homolog-like (yeast) 1176 +/- 55 1011 +/- 26 1124 +/- 72 1062 +/- 68 -0.029640857 -0.549900158 0.012026184 0.935882182 TPT1 tumor protein, translationally-controlled 1 440 +/- 33 330 +/- 27 527 +/- 42 469 +/- 48 -0.028534908 -0.385138793 0.031200246 0.417830754 TRAPPC5 trafficking protein particle complex 5 2581 +/- 63 2125 +/- 116 2463 +/- 162 2219 +/- 88 -0.009296312 -0.223796618 0.024411641 0.727839766 TSGA2 testis specific A2 homolog (mouse) 658 +/- 150 274 +/- 41 370 +/- 40 239 +/- 30 -0.049773829 -0.027797187 0.044111728 0.113750324 TSPAN3 tetraspanin 3 4001 +/- 86 3629 +/- 136 3966 +/- 66 3699 +/- 96 -0.047447907 -0.053879539 0.0498443 0.24013768 TUBB tubulin, beta 14019 +/- 320 12891 +/- 352 13789 +/- 333 13992 +/- 673 -0.039424516 0.79545786 0.011281322 0.450487648 TUBB2A tubulin, beta 2A 32505 +/- 1058 29201 +/- 789 30330 +/- 702 30704 +/- 944 -0.033009461 0.759243251 0.016857205 0.639808342 TUSC3 tumor suppressor candidate 3 593 +/- 43 437 +/- 30 743 +/- 35 545 +/- 36 -0.015208368 -0.003574256 0.026811227 0.078995526 TUSC4 tumor suppressor candidate 4 680 +/- 15 597 +/- 17 608 +/- 25 633 +/- 24 -0.004189125 0.477031885 0.001727243 0.830559707 UBE2J2 ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast) 433 +/- 20 329 +/- 22 433 +/- 21 410 +/- 38 -0.005391625 -0.608340745 0.032680246 0.946323589 UGCG UDP-glucose ceramide glucosyltransferase 1454 +/- 63 1024 +/- 64 1638 +/- 69 1487 +/- 121 -0.000733354 -0.318253979 0.001066394 0.429596549 USP4 ubiquitin specific peptidase 4 (proto-oncogene) 2228 +/- 74 1806 +/- 34 2258 +/- 99 1973 +/- 65 -0.001323917 -0.04187396 0.015351275 0.35433655 UTP11L UTP11-like, U3 small nucleolar ribonucleoprotein, (yeast) 1063 +/- 52 748 +/- 78 1102 +/- 32 926 +/- 140 -0.009003513 -0.282294484 0.015666919 0.292952341 VEGF vascular endothelial growth factor 2713 +/- 90 2084 +/- 104 2439 +/- 134 2150 +/- 54 -0.001085404 -0.088853654 0.00811421 0.144813071 VEZT vezatin, adherens junctions transmembrane protein 486 +/- 18 420 +/- 10 474 +/- 38 465 +/- 24 -0.012320014 -0.832441471 0.008056112 0.576774112 VPS37A vacuolar protein sorting 37 homolog A (S. cerevisiae) 350 +/- 19 271 +/- 22 344 +/- 18 318 +/- 14 -0.022864292 -0.295474925 0.047238596 0.071244663 WBSCR17 Williams-Beuren syndrome chromosome region 17 3846 +/- 222 2759 +/- 329 4476 +/- 273 3946 +/- 239 -0.023532811 -0.178057196 0.009503065 0.539811253 WEE1 WEE1 homolog (S. pombe) 156 +/- 15 113 +/- 11 162 +/- 19 156 +/- 13 -0.043432583 -0.792522934 0.027429744 0.31484471 YIPF5 Yip1 domain family, member 5 2776 +/- 72 2388 +/- 136 2883 +/- 102 2798 +/- 74 -0.037028446 -0.516929185 0.008488735 0.633725015 YRDC yrdC domain containing (E. coli) 396 +/- 15 305 +/- 14 466 +/- 9 394 +/- 30 -0.001409124 -0.075638283 0.00273562 0.423805793 ZC3H13 Zinc finger CCCH-type containing 13 2082 +/- 90 1605 +/- 162 1861 +/- 75 1362 +/- 193 -0.033399587 -0.058960157 0.01510726 0.01453634 ZNF503 zinc finger protein 503 989 +/- 27 822 +/- 31 1260 +/- 34 1165 +/- 60 -0.002238555 -0.214355025 0.001712092 0.186621246 ZNF527 zinc finger protein 527 174 +/- 9 109 +/- 12 181 +/- 11 142 +/- 18 -0.001325718 -0.106890278 0.038710439 0.463509012 ZWINT ZW10 interactor 592 +/- 49 356 +/- 55 462 +/- 44 332 +/- 21 -0.009324758 -0.032175899 0.007406112 0.088879118 Upregulated with Age and Correlated with MSI in DG Region ANKRD37 ankyrin repeat domain 37 571 +/- 66 630 +/- 24 455 +/- 38 593 +/- 37 0.432741945 0.027995366 0.412894238 0.018785119 ANP32B acidic (leucine-rich) nuclear phosphoprotein 32 family, member B 5261 +/- 630 6605 +/- 629 4342 +/- 135 4863 +/- 137 0.162024543 0.024181775 0.065918043 0.032732518 APBB1 amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) 5027 +/- 303 5203 +/- 381 4570 +/- 115 4883 +/- 59 0.724592165 0.044144337 0.539062016 0.031690336 APOA1BP apolipoprotein A-I binding protein 3444 +/- 146 3736 +/- 191 2627 +/- 74 3301 +/- 117 0.25395052 0.001861122 0.215036906 0.033290898

S4- MSI correlated and non-correlated ARGs.xls Page 15 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG APOD apolipoprotein D 1694 +/- 156 2698 +/- 413 1143 +/- 60 1879 +/- 176 0.060518899 0.011046198 0.019968251 0.009951874 ARHGEF2 rho/rac guanine nucleotide exchange factor (GEF) 2 667 +/- 82 848 +/- 50 714 +/- 18 958 +/- 49 0.093233964 0.00534307 0.103134121 0.022693676 BTG4 B-cell translocation gene 4 106 +/- 9 193 +/- 31 105 +/- 8 196 +/- 11 0.035256012 0.000213402 0.093942226 0.005027946 CCDC49 coiled-coil domain containing 49 276 +/- 18 313 +/- 20 246 +/- 12 319 +/- 21 0.202111398 0.01911797 0.097528639 0.047103103 CCDC74A coiled-coil domain containing 74A 613 +/- 51 646 +/- 93 640 +/- 36 1150 +/- 118 0.764731904 0.010155303 0.43691184 0.007534103 CCDC99 Coiled-coil domain containing 99 843 +/- 108 1220 +/- 192 518 +/- 27 688 +/- 24 0.125529209 0.00116804 0.042277227 0.024707287 CD2BP2 CD2 (cytoplasmic tail) binding protein 2 434 +/- 27 533 +/- 24 382 +/- 9 508 +/- 28 0.022462219 0.007719463 0.191814082 0.022361307 CD44 CD44 molecule (Indian blood group) 390 +/- 49 579 +/- 97 642 +/- 74 919 +/- 44 0.124377723 0.01296357 0.21306577 0.038642091 CD58 CD58 molecule 179 +/- 17 277 +/- 45 140 +/- 9 195 +/- 13 0.084202317 0.010654824 0.017459327 0.005573134 CHM choroideremia (Rab escort protein 1) 1904 +/- 130 2177 +/- 95 1862 +/- 132 2272 +/- 98 0.123267318 0.03547205 0.087660304 0.037080165 CHST3 carbohydrate (chondroitin 6) sulfotransferase 3 639 +/- 84 773 +/- 125 385 +/- 5 482 +/- 26 0.39835933 0.019465109 0.175896944 0.044542366 CLCC1 chloride channel CLIC-like 1 332 +/- 29 441 +/- 25 310 +/- 14 395 +/- 5 0.017994612 0.001144694 0.066524997 0.011344007 CNDP1 carnosine dipeptidase 1 (metallopeptidase M20 family) 1872 +/- 548 3446 +/- 569 974 +/- 183 2032 +/- 172 0.074299685 0.002279399 0.043147819 0.006866974 CNP 2',3'-cyclic nucleotide 3' phosphodiesterase 8116 +/- 1232 11141 +/- 2317 4506 +/- 307 5837 +/- 379 0.283978684 0.025495228 0.095452694 0.008006476 COL1A2 collagen, type I, alpha 2 214 +/- 68 127 +/- 38 73 +/- 10 162 +/- 21 -0.292040139 0.010047955 0.103002646 0.018428844 CPSF2 cleavage and polyadenylation specific factor 2, 100kDa 526 +/- 47 668 +/- 60 737 +/- 35 880 +/- 49 0.094333194 0.046656248 0.048092696 0.032414627 CSTB cystatin B (stefin B) 6756 +/- 454 7724 +/- 450 6112 +/- 199 7133 +/- 147 0.161022131 0.002746244 0.261414852 0.040797159 CUGBP2 CUG triplet repeat, RNA binding protein 2 1263 +/- 229 2289 +/- 319 1772 +/- 270 3161 +/- 347 0.028023032 0.01349226 0.240528635 0.044816364 DBNDD2 dysbindin (dystrobrevin binding protein 1) domain containing 2 6719 +/- 435 8157 +/- 929 5664 +/- 297 6675 +/- 313 0.202988558 0.044247121 0.04992539 0.007616374 DYM dymeclin 885 +/- 42 957 +/- 31 771 +/- 20 925 +/- 49 0.201292567 0.030224595 0.511550316 0.019298431 ECD ecdysoneless homolog (Drosophila) 625 +/- 49 805 +/- 64 622 +/- 29 839 +/- 52 0.05185547 0.009973563 0.008062515 0.024874934 EDF1 endothelial differentiation-related factor 1 2924 +/- 89 3195 +/- 140 2664 +/- 103 3111 +/- 63 0.139315885 0.006026401 0.052477922 0.002808541 EDG2 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 2 5091 +/- 735 6481 +/- 945 2992 +/- 289 3972 +/- 198 0.274115823 0.022001652 0.052812676 0.027660984 EIF3S7 eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa 2662 +/- 54 2791 +/- 67 2401 +/- 59 2691 +/- 31 0.165781836 0.002838447 0.286051699 0.005906782 EIF4G3 eukaryotic translation initiation factor 4 gamma, 3 3958 +/- 161 4220 +/- 172 3636 +/- 92 4030 +/- 135 0.29217516 0.045357345 0.468616205 0.001540949 FA2H fatty acid 2-hydroxylase 1088 +/- 162 1680 +/- 349 622 +/- 81 901 +/- 73 0.166860843 0.030926943 0.027447452 0.011595959 FARP2 FERM, RhoGEF and pleckstrin domain protein 2 207 +/- 32 348 +/- 65 210 +/- 30 315 +/- 16 0.092947123 0.015546132 0.005267419 0.018364588 FBLN1 fibulin 1 358 +/- 58 415 +/- 18 289 +/- 15 381 +/- 10 0.381766064 0.000673864 0.224958679 0.011025943 FBP1 fructose-1,6-bisphosphatase 1 89 +/- 10 125 +/- 32 81 +/- 8 136 +/- 13 0.323201833 0.009196429 0.811673685 0.014497467 FCER1G Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide 283 +/- 30 393 +/- 40 195 +/- 12 277 +/- 15 0.054457432 0.002515946 0.032056759 0.00741832 FEZ1 fasciculation and elongation protein zeta 1 (zygin I) 8114 +/- 604 9755 +/- 837 6969 +/- 218 8397 +/- 232 0.146056529 0.00163515 0.051189539 0.002001414 FLII flightless I homolog (Drosophila) 1073 +/- 71 1217 +/- 48 871 +/- 23 1050 +/- 35 0.128484081 0.003355357 0.162496521 0.031848714 FLJ25530 hepatocyte cell adhesion molecule 4362 +/- 359 5227 +/- 460 3822 +/- 130 4496 +/- 91 0.171005961 0.002411059 0.064663008 0.003095961 FLJ41131 FLJ41131 protein 131 +/- 16 159 +/- 16 101 +/- 4 144 +/- 10 0.24715985 0.010711903 0.367893125 0.001668931 FLNC filamin C, gamma (actin binding protein 280) 423 +/- 64 485 +/- 58 196 +/- 21 278 +/- 15 0.485099269 0.012306199 0.464470525 0.049669847 FLT3 fms-related tyrosine kinase 3 200 +/- 23 262 +/- 27 110 +/- 9 165 +/- 6 0.121667713 0.000867473 0.16108864 0.042516895 FOXO3A Forkhead box O3A 331 +/- 35 512 +/- 68 283 +/- 27 375 +/- 22 0.048674426 0.026860825 0.057457718 0.021827275 FTSJ2 FtsJ homolog 2 (E. coli) 498 +/- 18 476 +/- 31 406 +/- 8 463 +/- 17 -0.54816672 0.027040346 0.154892688 0.031353995 GFM1 G elongation factor, mitochondrial 1 175 +/- 15 233 +/- 22 216 +/- 6 265 +/- 11 0.063729251 0.006751872 0.172655706 0.000637894 GMPS guanine monphosphate synthetase 570 +/- 45 619 +/- 27 438 +/- 20 530 +/- 21 0.377687094 0.011126804 0.436972827 0.040622754 GOPC golgi associated PDZ and coiled-coil motif containing 462 +/- 34 622 +/- 43 560 +/- 32 730 +/- 26 0.015844249 0.002572784 0.067332845 0.008610876 GPKOW G patch domain and KOW motifs 740 +/- 27 793 +/- 31 690 +/- 27 787 +/- 32 0.234900045 0.048299019 0.302033046 0.022966989 HECTD2 HECT domain containing 2 1579 +/- 126 2196 +/- 109 1410 +/- 84 1984 +/- 71 0.004135061 0.000568656 0.080773087 0.041573814 HEXDC hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing 148 +/- 17 180 +/- 18 173 +/- 20 274 +/- 24 0.22618189 0.011703808 0.446919739 0.006790846

S4- MSI correlated and non-correlated ARGs.xls Page 16 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG HIPK2 Homeodomain interacting protein kinase 2 13628 +/- 1528 17700 +/- 1910 8772 +/- 365 10920 +/- 395 0.12841218 0.003366906 0.012033369 0.002874401 HKR2 GLI-Kruppel family member HKR2 186 +/- 15 198 +/- 14 187 +/- 10 237 +/- 13 0.583444126 0.017130869 0.930468698 0.018151489 HLA-DPB1 major histocompatibility complex, class II, DP beta 1 275 +/- 55 485 +/- 42 153 +/- 53 409 +/- 56 0.013814114 0.009354973 0.103180322 0.024830136 HNRPL heterogeneous nuclear ribonucleoprotein L 5232 +/- 292 6250 +/- 170 6161 +/- 225 7259 +/- 144 0.016730469 0.003234389 0.14241682 0.023284248 HRASLS3 HRAS-like suppressor 3 3691 +/- 429 4571 +/- 653 2117 +/- 93 2770 +/- 179 0.290075953 0.017367553 0.12189474 0.015225861 HS1BP3 HS1-binding protein 3 323 +/- 18 374 +/- 24 190 +/- 32 362 +/- 29 0.128946178 0.003199753 0.330957831 0.03315259 IL13RA1 interleukin 13 receptor, alpha 1 147 +/- 22 136 +/- 18 121 +/- 7 176 +/- 7 -0.703974387 0.000283542 0.737928322 0.045423183 IPO9 importin 9 1211 +/- 95 1365 +/- 81 1369 +/- 47 1573 +/- 68 0.243782177 0.042274605 0.274607502 0.003378761 IVNS1ABP influenza virus NS1A binding protein 993 +/- 80 1097 +/- 80 745 +/- 17 848 +/- 36 0.378345922 0.043123029 0.270369883 0.002940165 LASS2 LAG1 longevity assurance homolog 2 (S. cerevisiae) 1543 +/- 159 2019 +/- 247 1012 +/- 58 1354 +/- 79 0.14174194 0.008588905 0.014698451 0.016697618 LIMS1 LIM and senescent cell antigen-like domains 1 815 +/- 63 1059 +/- 110 676 +/- 38 871 +/- 66 0.090147838 0.040072862 0.017402257 0.004815562 LSM4 LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae) 1636 +/- 167 1825 +/- 54 1373 +/- 9 1750 +/- 75 0.320236054 0.007012113 0.495714106 0.003639407 MAP4K5 mitogen-activated protein kinase kinase kinase kinase 5 695 +/- 83 1080 +/- 188 670 +/- 51 919 +/- 70 0.103657814 0.021575168 0.009082356 0.003570937 MAPRE1 microtubule-associated protein, RP/EB family, member 1 1826 +/- 135 2236 +/- 182 1327 +/- 73 1595 +/- 31 0.103330607 0.012843845 0.022725514 0.028923787 MAST4 microtubule associated serine/threonine kinase family member 4 163 +/- 7 143 +/- 21 168 +/- 2 203 +/- 10 -0.408933212 0.022907686 0.214095482 0.001821582 MCAM melanoma cell adhesion molecule 1170 +/- 212 1662 +/- 293 589 +/- 48 817 +/- 61 0.207534483 0.018798175 0.088472398 0.031657183 MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein 113 +/- 18 175 +/- 17 138 +/- 12 186 +/- 14 0.034417289 0.029901837 0.139021258 0.017434983 MFN2 mitofusin 2 4230 +/- 255 4976 +/- 285 3779 +/- 52 4823 +/- 275 0.080030538 0.017920767 0.228859693 0.015714214 MRPL20 mitochondrial ribosomal protein L20 2020 +/- 172 2410 +/- 102 1610 +/- 52 1867 +/- 59 0.086342782 0.010571197 0.173913401 0.015138317 MRPL54 mitochondrial ribosomal protein L54 455 +/- 104 726 +/- 46 610 +/- 107 982 +/- 113 0.049291972 0.041065514 0.054501423 0.04139206 MRPS22 mitochondrial ribosomal protein S22 836 +/- 43 995 +/- 69 810 +/- 40 988 +/- 24 0.084501056 0.005432205 0.105056253 0.004363638 MXI1 MAX interactor 1 6342 +/- 614 7312 +/- 652 4531 +/- 138 5071 +/- 166 0.30407088 0.03629269 0.110119976 0.03513731 MYEOV2 myeloma overexpressed 2 11575 +/- 867 12859 +/- 629 9590 +/- 539 11808 +/- 395 0.260852182 0.009414064 0.388653459 0.034130899 NCKIPSD NCK interacting protein with SH3 domain 1420 +/- 128 2242 +/- 157 1411 +/- 94 2164 +/- 111 0.002447617 0.000744802 0.093724422 0.031940681 NDRG1 N-myc downstream regulated gene 1 6429 +/- 896 7904 +/- 978 4264 +/- 354 5467 +/- 340 0.292367088 0.036858064 0.141501528 0.025734988 NDUFB8 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa 11769 +/- 658 13321 +/- 391 9711 +/- 250 12186 +/- 194 0.076374256 2.96615E-05 0.311302957 0.018293507 NIFUN NifU-like N-terminal domain containing 10519 +/- 373 10858 +/- 390 9520 +/- 257 10727 +/- 229 0.543420828 0.006639926 0.79585811 0.028525289 NINJ2 ninjurin 2 672 +/- 49 990 +/- 158 361 +/- 35 494 +/- 25 0.104260321 0.014367225 0.010782712 0.03444998 NOP17 NOP17 882 +/- 62 1108 +/- 85 828 +/- 45 1014 +/- 32 0.060040148 0.008849685 0.258957949 0.029022383 NPC1 Niemann-Pick disease, type C1 1631 +/- 195 2450 +/- 429 909 +/- 54 1244 +/- 72 0.125565112 0.006001267 0.015047686 0.004993016 NR2F1 Nuclear receptor subfamily 2, group F, member 1 3880 +/- 234 4227 +/- 278 4775 +/- 257 5717 +/- 210 0.362878868 0.019733476 0.649312745 0.043826958 NRBP2 nuclear receptor binding protein 2 3166 +/- 354 3523 +/- 368 2980 +/- 110 3462 +/- 135 0.500961601 0.023661664 0.355251994 0.011977703 NUP62 nucleoporin 62kDa 885 +/- 11 1158 +/- 43 1053 +/- 49 1241 +/- 35 0.000988177 0.012690922 0.069716696 0.04182895 OGG1 8-oxoguanine DNA glycosylase 301 +/- 28 351 +/- 24 298 +/- 15 364 +/- 22 0.209517684 0.039467584 0.347236884 0.00046475 OGN osteoglycin (osteoinductive factor, mimecan) 1651 +/- 630 839 +/- 341 397 +/- 58 709 +/- 94 -0.290932179 0.029188234 0.085982736 0.001225276 OSBPL1A oxysterol binding protein-like 1A 5228 +/- 458 5797 +/- 389 3578 +/- 80 4173 +/- 148 0.366520276 0.011532966 0.309880559 0.005996347 PB1 polybromo 1 2458 +/- 285 3086 +/- 101 2471 +/- 95 3125 +/- 70 0.081741937 0.000415029 0.13758296 0.00634582 PCDHGB6 protocadherin gamma subfamily B, 6 4504 +/- 348 5671 +/- 569 2978 +/- 131 3473 +/- 103 0.116814424 0.01610839 0.074867716 0.044674878 PCGF5 polycomb group ring finger 5 241 +/- 31 382 +/- 71 220 +/- 18 310 +/- 16 0.112898328 0.004382423 0.024573012 0.016707046 PGAM1 phosphoglycerate mutase 1 (brain) 11785 +/- 417 13568 +/- 474 10224 +/- 320 12402 +/- 471 0.018304155 0.006017659 0.146932074 0.039091516 PGAM2 phosphoglycerate mutase 2 (muscle) 175 +/- 19 219 +/- 19 163 +/- 19 222 +/- 6 0.125170277 0.024152411 0.121442235 0.049178303 PIGZ phosphatidylinositol glycan, class Z 644 +/- 33 916 +/- 90 686 +/- 41 941 +/- 35 0.027433302 0.001051162 0.094046979 0.015991583 PITPNC1 Phosphatidylinositol transfer protein, cytoplasmic 1 130 +/- 18 150 +/- 13 145 +/- 6 207 +/- 21 0.384142247 0.036598477 0.126519927 0.030402242 PLA2G4C phospholipase A2, group IVC (cytosolic, calcium-independent) 321 +/- 37 368 +/- 21 312 +/- 19 375 +/- 20 0.310811668 0.048104443 0.80414807 0.048550323

S4- MSI correlated and non-correlated ARGs.xls Page 17 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG PLEKHB1 pleckstrin homology domain containing, family B (evectins) member 1 6842 +/- 885 9643 +/- 1345 4705 +/- 239 6453 +/- 287 0.117438066 0.001452148 0.025513329 0.005928906 PLTP phospholipid transfer protein 1127 +/- 38 1268 +/- 54 1124 +/- 55 1435 +/- 28 0.062018148 0.001337345 0.017842124 0.031647432 POLR2G polymerase (RNA) II (DNA directed) polypeptide G 1501 +/- 46 1883 +/- 158 1045 +/- 54 1288 +/- 36 0.060342218 0.005367014 0.02135662 0.038968732 PPM1B protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform 2968 +/- 253 3629 +/- 258 2741 +/- 80 2996 +/- 47 0.097261893 0.025430299 0.036772445 0.00983383 PRDM2 PR domain containing 2, with ZNF domain 347 +/- 45 386 +/- 34 436 +/- 22 557 +/- 23 0.510514203 0.00400849 0.540143915 0.004554965 PRSS21 protease, serine, 21 (testisin) 154 +/- 18 182 +/- 13 167 +/- 4 246 +/- 21 0.22769378 0.01911103 0.026257987 2.4539E-05 PSMB4 proteasome (prosome, macropain) subunit, beta type, 4 3705 +/- 115 3631 +/- 124 3280 +/- 73 3573 +/- 83 -0.669068417 0.027906833 0.995437927 0.041657788 PTDSS2 phosphatidylserine synthase 2 563 +/- 47 649 +/- 35 490 +/- 18 620 +/- 48 0.179105234 0.049206921 0.267643085 0.015072598 PTPN21 Protein tyrosine phosphatase, non-receptor type 21 156 +/- 37 305 +/- 48 153 +/- 15 276 +/- 31 0.036045999 0.013348896 0.104382719 0.018237476 PVALB parvalbumin 391 +/- 24 469 +/- 50 287 +/- 22 418 +/- 28 0.199547166 0.005841855 0.577157677 0.033436226 RECQL5 RecQ protein-like 5 88 +/- 21 145 +/- 16 75 +/- 7 129 +/- 9 0.059387086 0.001052589 0.010148919 0.03932201 RGS11 regulator of G-protein signalling 11 262 +/- 54 525 +/- 62 397 +/- 50 606 +/- 54 0.009410819 0.019498241 0.076190609 0.026970839 RHOU ras homolog gene family, member U 2390 +/- 265 3326 +/- 556 1311 +/- 72 1705 +/- 52 0.171162106 0.001850475 0.02871214 0.002801328 RICTOR rapamycin-insensitive companion of mTOR 2415 +/- 271 3115 +/- 219 2053 +/- 54 2477 +/- 109 0.073289041 0.013265709 0.061739465 0.00307475 RIF1 RAP1 interacting factor homolog (yeast) 637 +/- 107 956 +/- 124 515 +/- 28 671 +/- 28 0.080334997 0.003329323 0.015631866 0.017656192 RNASE4 ribonuclease, RNase A family, 4 204 +/- 17 250 +/- 22 133 +/- 12 240 +/- 28 0.132486179 0.015739107 0.285022576 0.002475872 RNASEH1 ribonuclease H1 129 +/- 15 178 +/- 14 148 +/- 7 241 +/- 18 0.041606548 0.00463307 0.229431039 0.032545623 RPESP RPE-spondin 216 +/- 36 219 +/- 54 370 +/- 37 509 +/- 43 0.956505713 0.036551267 0.721528508 0.017494422 RPL30 ribosomal protein L30 15248 +/- 656 16050 +/- 750 15243 +/- 600 17322 +/- 644 0.439732192 0.043330173 0.616251572 0.023878632 SCD5 stearoyl-CoA desaturase 5 15204 +/- 701 15897 +/- 556 13728 +/- 231 15363 +/- 292 0.45778559 0.002264217 0.312980261 0.046254337 SCRN1 secernin 1 9838 +/- 238 10891 +/- 605 8473 +/- 141 10001 +/- 220 0.152799039 0.000635404 0.810288859 0.017126232 SEC23B Sec23 homolog B (S. cerevisiae) 1038 +/- 61 1069 +/- 58 923 +/- 23 1057 +/- 33 0.717897621 0.010600432 0.77028712 0.015610918 SEC61G Sec61 gamma subunit 2171 +/- 111 2251 +/- 109 1907 +/- 76 2224 +/- 48 0.617326901 0.007253404 0.725738304 0.04893153 SERPIND1 serpin peptidase inhibitor, clade D (heparin cofactor), member 1 237 +/- 137 92 +/- 52 51 +/- 5 74 +/- 6 -0.356911899 0.023889551 0.129143378 0.030288553 SETX senataxin 1154 +/- 76 1267 +/- 18 1110 +/- 19 1244 +/- 44 0.203689114 0.033305531 0.214133904 6.06149E-05 SIN3B SIN3 homolog B, transcription regulator (yeast) 1456 +/- 63 1539 +/- 57 1516 +/- 56 1759 +/- 48 0.346477719 0.009402062 0.994184813 0.022693048 SLC13A4 solute carrier family 13 (sodium/sulfate symporters), member 4 758 +/- 418 154 +/- 92 35 +/- 8 120 +/- 24 -0.212460871 0.019909476 0.227434885 0.00594441 SLC15A3 solute carrier family 15, member 3 255 +/- 27 313 +/- 19 234 +/- 7 271 +/- 8 0.112498835 0.005854163 0.10311742 0.006223589 SLC22A18 solute carrier family 22 (organic cation transporter), member 18 269 +/- 23 400 +/- 39 191 +/- 11 317 +/- 21 0.019838905 0.001619511 0.11369647 0.044955166 SLC25A18 solute carrier family 25 (mitochondrial carrier), member 18 2127 +/- 291 3200 +/- 170 2193 +/- 184 3259 +/- 127 0.01291412 0.001186179 0.050441623 0.014997487 SLC45A3 solute carrier family 45, member 3 194 +/- 24 304 +/- 46 108 +/- 28 201 +/- 22 0.067549211 0.027744422 0.044850369 0.022020153 SLC5A11 solute carrier family 5 (sodium/glucose cotransporter), member 11 675 +/- 128 1122 +/- 169 389 +/- 31 607 +/- 40 0.063233045 0.002557526 0.050659734 0.012914146 SNX9 sorting nexin 9 1081 +/- 34 1199 +/- 60 1155 +/- 37 1282 +/- 40 0.125106773 0.045267651 0.020086567 0.049494643 SOHLH2 spermatogenesis and oogenesis specific basic helix-loop-helix 2 101 +/- 10 163 +/- 15 88 +/- 6 159 +/- 11 0.007052751 0.001346771 0.113962623 0.033681699 SPINK2 serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor) 187 +/- 45 123 +/- 5 124 +/- 3 177 +/- 17 -0.21418252 0.03742661 0.316945269 0.020681757 SRBD1 S1 RNA binding domain 1 122 +/- 36 182 +/- 29 85 +/- 6 173 +/- 17 0.229268598 0.004933397 0.17580218 0.002778312 SRP19 signal recognition particle 19kDa 2698 +/- 166 3239 +/- 67 2572 +/- 101 3210 +/- 30 0.021094707 0.001032739 0.112768564 0.012163432 ST8SIA3 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3 2875 +/- 542 3178 +/- 335 2260 +/- 206 3420 +/- 132 0.6458159 0.001363044 0.860166309 0.034540252 SURF1 surfeit 1 1906 +/- 52 2026 +/- 101 1667 +/- 86 1959 +/- 45 0.32405183 0.018134614 0.730804716 0.033267111 TAF1B TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa 234 +/- 23 246 +/- 38 330 +/- 22 422 +/- 23 0.789191471 0.01950325 0.892131828 0.042500346 TBC1D17 TBC1 domain family, member 17 1262 +/- 103 1539 +/- 80 1047 +/- 42 1261 +/- 39 0.061520457 0.004927262 0.085410185 0.042209106 TF transferrin 7019 +/- 1463 9974 +/- 1622 4189 +/- 354 6128 +/- 572 0.206185233 0.02417124 0.113059721 0.010764522 THNSL1 threonine synthase-like 1 (bacterial) 904 +/- 69 911 +/- 73 1077 +/- 20 1202 +/- 28 0.947204565 0.007607965 0.707473187 0.044875816 TMED4 transmembrane emp24 protein transport domain containing 4 1134 +/- 55 1431 +/- 104 1087 +/- 50 1361 +/- 59 0.036499694 0.007200925 0.077299985 0.024735739

S4- MSI correlated and non-correlated ARGs.xls Page 18 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG TMEM87B Transmembrane protein 87B 96 +/- 30 190 +/- 26 87 +/- 22 198 +/- 10 0.039793595 0.002630566 0.180729609 0.020908784 TNFAIP6 tumor necrosis factor, alpha-induced protein 6 258 +/- 31 467 +/- 111 161 +/- 13 251 +/- 29 0.122884819 0.031791821 0.01280199 0.043042898 TNFSF13B tumor necrosis factor (ligand) superfamily, member 13b 429 +/- 38 458 +/- 44 416 +/- 30 552 +/- 32 0.634272516 0.013214316 0.36760737 0.023048356 TNIP2 TNFAIP3 interacting protein 2 474 +/- 48 575 +/- 35 439 +/- 19 505 +/- 20 0.124972994 0.04206126 0.317968208 0.042891991 TNXA tenascin XA pseudogene 497 +/- 80 915 +/- 160 295 +/- 39 476 +/- 26 0.05061517 0.004206738 0.009838066 0.007970109 TSEN34 tRNA splicing endonuclease 34 homolog (S. cerevisiae) 991 +/- 52 1198 +/- 77 784 +/- 49 985 +/- 39 0.052950505 0.010934018 0.353018347 0.022477243 TTBK2 Tau tubulin kinase 2 1287 +/- 128 1604 +/- 88 1035 +/- 99 1388 +/- 79 0.071933506 0.020980087 0.049383808 0.01207405 UBE2G1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) 643 +/- 51 771 +/- 63 502 +/- 12 695 +/- 17 0.150032006 2.3432E-05 0.316364933 0.003208418 USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) 1553 +/- 103 1788 +/- 135 1582 +/- 72 1981 +/- 114 0.198756158 0.021015637 0.28450932 0.001748003 VTI1B vesicle transport through interaction with t-SNAREs homolog 1B (yeast) 3503 +/- 235 3273 +/- 106 2823 +/- 159 3297 +/- 104 -0.403886212 0.03646993 0.276102219 0.025734931 WAC WW domain containing adaptor with coiled-coil 3946 +/- 99 4045 +/- 77 3700 +/- 73 4022 +/- 31 0.450134062 0.005533709 0.34289359 0.032309994 WASPIP Wiskott-Aldrich syndrome protein interacting protein 453 +/- 53 836 +/- 159 339 +/- 48 483 +/- 31 0.061546427 0.036626616 0.005607277 0.036228076 WDR25 WD repeat domain 25 360 +/- 15 348 +/- 18 272 +/- 4 365 +/- 15 -0.619300214 0.002078102 0.272477023 0.00340984 WNT5A wingless-type MMTV integration site family, member 5A 485 +/- 51 763 +/- 107 574 +/- 90 877 +/- 81 0.050403523 0.03413742 0.005573256 0.017567726 XTP3TPA XTP3-transactivated protein A 1242 +/- 57 1263 +/- 36 1182 +/- 54 1352 +/- 33 0.768388513 0.027719462 0.563452935 0.045011376 ZNF323 zinc finger protein 323 162 +/- 15 187 +/- 22 236 +/- 13 296 +/- 17 0.368404035 0.023072962 0.684820285 0.023117495 ZNF384 zinc finger protein 384 657 +/- 34 714 +/- 19 682 +/- 19 758 +/- 21 0.175514622 0.026242577 0.059700083 0.048969101 ZNF561 zinc finger protein 561 444 +/- 41 512 +/- 38 329 +/- 24 397 +/- 16 0.249224449 0.046430515 0.617955954 0.044104515 ZNF564 zinc finger protein 564 324 +/- 32 430 +/- 24 307 +/- 19 390 +/- 12 0.02532311 0.006175825 0.087713265 0.046735044 ZYG11B Zyg-11 homolog B (C. elegans) 783 +/- 95 1096 +/- 114 916 +/- 54 1329 +/- 49 0.062752735 0.00030351 0.282315169 0.009115068 Downregulated with Age and Correlated with MSI in DG Region ACO1 aconitase 1, soluble 964 +/- 82 811 +/- 43 950 +/- 45 803 +/- 46 -0.139533162 -0.048937589 0.118328259 0.028974705 ALS2CR7 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7 167 +/- 14 134 +/- 8 171 +/- 14 126 +/- 13 -0.070816985 -0.048405733 0.06933312 0.00901095 AP4E1 adaptor-related protein complex 4, epsilon 1 subunit 302 +/- 25 267 +/- 10 347 +/- 7 261 +/- 13 -0.231271577 -0.000873394 0.897469271 0.010348028 ARHGAP21 Rho GTPase activating protein 21 696 +/- 46 796 +/- 50 840 +/- 34 715 +/- 22 0.175529423 -0.014325633 0.124542423 0.033989336 ARHGEF11 Rho guanine nucleotide exchange factor (GEF) 11 658 +/- 70 564 +/- 45 969 +/- 43 769 +/- 57 -0.290212086 -0.023698091 0.254470245 0.049894591 ARHGEF12 Rho guanine nucleotide exchange factor (GEF) 12 5718 +/- 162 5133 +/- 235 5709 +/- 82 5041 +/- 202 -0.07092767 -0.025744466 0.01647002 0.000352056 ARMC2 armadillo repeat containing 2 247 +/- 26 192 +/- 9 249 +/- 6 220 +/- 6 -0.09735098 -0.008670542 0.110501317 0.002769445 ASAHL N-acylsphingosine amidohydrolase (acid ceramidase)-like 157 +/- 9 116 +/- 12 192 +/- 21 128 +/- 16 -0.026696429 -0.03809744 0.059545479 0.032905844 ASF1A ASF1 anti-silencing function 1 homolog A (S. cerevisiae) 389 +/- 32 477 +/- 23 500 +/- 19 435 +/- 10 0.053544301 -0.01673303 0.025333342 0.019574443 B3GNT1 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 7168 +/- 286 6332 +/- 368 7310 +/- 119 6286 +/- 200 -0.105163388 -0.003594559 0.052310393 0.004419268 BCL10 B-cell CLL/lymphoma 10 367 +/- 32 332 +/- 22 459 +/- 20 380 +/- 15 -0.39595478 -0.011414456 0.365470865 0.046034833 BOP1 block of proliferation 1 292 +/- 18 210 +/- 26 357 +/- 15 258 +/- 9 -0.026404469 -0.000563916 0.093683447 0.002243383 BRWD2 bromodomain and WD repeat domain containing 2 564 +/- 69 382 +/- 3 593 +/- 41 404 +/- 22 -0.046177058 -0.004233115 0.137620747 0.03576959 BTBD6 BTB (POZ) domain containing 6 118 +/- 4 104 +/- 8 134 +/- 8 104 +/- 6 -0.158310164 -0.01535663 0.060607861 0.014502969 BXDC5 brix domain containing 5 1465 +/- 71 1335 +/- 55 1579 +/- 58 1297 +/- 45 -0.179780032 -0.004050566 0.390284423 0.038802183 CABYR calcium binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) 307 +/- 34 240 +/- 11 269 +/- 21 207 +/- 8 -0.109651154 -0.032138739 0.104909416 0.017114413 CAMKK2 calcium/calmodulin-dependent protein kinase kinase 2, beta 2835 +/- 306 2109 +/- 116 2217 +/- 96 1716 +/- 125 -0.065711977 -0.01294662 0.016846478 0.027770538 CAMTA1 calmodulin binding transcription activator 1 3071 +/- 288 2648 +/- 114 3284 +/- 169 2656 +/- 62 -0.218065498 -0.011942988 0.167893933 0.024531737 CAST1 CAZ-associated structural protein 3281 +/- 360 2648 +/- 269 4391 +/- 181 3526 +/- 194 -0.191949962 -0.010196399 0.208325984 0.027611856 CBL Cas-Br-M (murine) ecotropic retroviral transforming sequence 1129 +/- 118 865 +/- 92 1148 +/- 81 844 +/- 74 -0.109052861 -0.021736039 0.131433734 0.010293181 CCDC86 coiled-coil domain containing 86 471 +/- 31 440 +/- 12 478 +/- 9 411 +/- 12 -0.386079118 -0.00223735 0.340722199 0.02763245 CCDC95 coiled-coil domain containing 95 936 +/- 37 859 +/- 28 1082 +/- 28 950 +/- 21 -0.126820418 -0.004763446 0.048753731 0.004658878 CD47 CD47 molecule 2438 +/- 231 1877 +/- 56 2572 +/- 107 2131 +/- 78 -0.059507365 -0.009185599 0.174884579 0.011047274

S4- MSI correlated and non-correlated ARGs.xls Page 19 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG CDH24 cadherin-like 24 969 +/- 98 925 +/- 70 890 +/- 56 690 +/- 25 -0.72626634 -0.014012972 0.643691656 0.00863681 CKLF chemokine-like factor 686 +/- 32 579 +/- 45 611 +/- 22 492 +/- 40 -0.086705955 -0.03746472 0.204254667 0.01971039 CNOT1 CCR4-NOT transcription complex, subunit 1 385 +/- 33 315 +/- 9 372 +/- 26 289 +/- 15 -0.091372506 -0.022822616 0.166544504 0.043704445 CRKL v-crk sarcoma virus CT10 oncogene homolog (avian)-like 1767 +/- 44 1717 +/- 67 1489 +/- 11 1401 +/- 15 -0.547481024 -0.001864775 0.992944647 0.009254949 CXADR Coxsackie virus and adenovirus receptor 2582 +/- 321 1752 +/- 139 2109 +/- 65 1507 +/- 97 -0.050205294 -0.001210434 0.044368516 0.013285592 DGCR6 DiGeorge syndrome critical region gene 6 979 +/- 27 906 +/- 45 1037 +/- 42 905 +/- 24 -0.196473797 -0.026267963 0.197307323 0.039225271 DMRTA2 DMRT-like family A2 171 +/- 21 95 +/- 12 262 +/- 37 117 +/- 11 -0.012484468 -0.009962376 0.053396251 0.020967938 DZIP1L DAZ interacting protein 1-like 133 +/- 14 134 +/- 14 213 +/- 16 147 +/- 7 0.975474003 -0.006560686 0.947943297 0.010398451 E2F3 E2F transcription factor 3 813 +/- 106 594 +/- 60 697 +/- 22 526 +/- 21 -0.111153911 -0.000358899 0.088442088 0.027741493 EDG1 endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 2404 +/- 126 2634 +/- 244 2878 +/- 139 2330 +/- 117 0.429359478 -0.014496804 0.449591648 0.017653786 EDG4 endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 4 173 +/- 16 166 +/- 12 211 +/- 12 168 +/- 8 -0.730365874 -0.014332489 0.875528657 0.01041439 EMX2 empty spiracles homolog 2 (Drosophila) 860 +/- 116 836 +/- 85 1476 +/- 75 1238 +/- 46 -0.874805619 -0.02744569 0.900047991 0.008143109 FANCM Fanconi anemia, complementation group M 187 +/- 20 162 +/- 14 232 +/- 6 158 +/- 16 -0.347210725 -0.007508077 0.511435978 0.019788744 FBXL14 F-box and leucine-rich repeat protein 14 1117 +/- 114 749 +/- 52 1272 +/- 40 979 +/- 38 -0.022120684 -0.000484356 0.075790002 0.03101781 FBXL3 F-box and leucine-rich repeat protein 3 2062 +/- 73 1887 +/- 100 2305 +/- 53 1970 +/- 46 -0.188586335 -0.000995486 0.153869078 0.00554673 FRMD4A FERM domain containing 4A 926 +/- 37 796 +/- 36 829 +/- 20 663 +/- 39 -0.03071271 -0.008664546 0.131708592 0.03971463 FSTL5 follistatin-like 5 790 +/- 173 669 +/- 70 1194 +/- 114 623 +/- 58 -0.537903012 -0.002692209 0.493975466 0.040086607 FUBP3 far upstream element (FUSE) binding protein 3 1363 +/- 127 1472 +/- 64 1328 +/- 43 1188 +/- 31 0.466008088 -0.026881377 0.273425732 0.025197501 FUT1 Fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, H blood group) 147 +/- 13 121 +/- 9 139 +/- 12 91 +/- 13 -0.13608644 -0.025097769 0.047727092 0.046486155 GABPA GA binding protein transcription factor, alpha subunit 60kDa 387 +/- 45 419 +/- 22 469 +/- 23 342 +/- 6 0.539252935 -0.001907202 0.438207969 0.026895072 GABRG3 gamma-aminobutyric acid (GABA) A receptor, gamma 3 83 +/- 11 74 +/- 14 144 +/- 6 100 +/- 6 -0.596888482 -0.0007611 0.435085853 0.000653098 GCAT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) 417 +/- 20 365 +/- 15 436 +/- 24 357 +/- 20 -0.071902485 -0.031788882 0.038505465 0.027924141 GCDH glutaryl-Coenzyme A dehydrogenase 441 +/- 27 371 +/- 17 418 +/- 24 317 +/- 19 -0.058952003 -0.009161825 0.030313498 0.000266268 GLI2 GLI-Kruppel family member GLI2 129 +/- 11 140 +/- 14 176 +/- 10 134 +/- 12 0.529673015 -0.024783634 0.377824723 0.001239906 GNMT glycine N-methyltransferase 171 +/- 11 138 +/- 16 174 +/- 20 111 +/- 11 -0.1268625 -0.024455407 0.255939058 0.02820895 GPR135 G protein-coupled receptor 135 154 +/- 8 126 +/- 19 203 +/- 9 163 +/- 8 -0.219929893 -0.012068476 0.509664873 0.021092649 GPR68 G protein-coupled receptor 68 143 +/- 27 149 +/- 7 197 +/- 12 151 +/- 16 0.844866887 -0.046931273 0.671536238 0.002239928 GTF2H5 general transcription factor IIH, polypeptide 5 1686 +/- 106 1502 +/- 113 1400 +/- 61 1199 +/- 50 -0.26041326 -0.030987164 0.28976154 0.022631442 H1F0 H1 histone family, member 0 1586 +/- 121 1504 +/- 164 1458 +/- 52 1178 +/- 25 -0.696287292 -0.0017378 0.435790097 0.008792147 H3F3A H3 histone, family 3A 1040 +/- 71 1128 +/- 59 1218 +/- 63 874 +/- 46 0.363583244 -0.001899474 0.179446199 0.013289081 HAGHL hydroxyacylglutathione hydrolase-like 559 +/- 53 491 +/- 35 436 +/- 21 337 +/- 5 -0.315099027 -0.00458053 0.402572973 0.014557226 HERC2P7 hect domain and RLD 2 pseudogene 7 170 +/- 14 126 +/- 21 225 +/- 17 160 +/- 19 -0.114895387 -0.033002361 0.110835988 0.013091111 HES1 hairy and enhancer of split 1, (Drosophila) 395 +/- 33 353 +/- 23 461 +/- 26 352 +/- 18 -0.327046892 -0.00802582 0.352841693 0.028114345 HES5 hairy and enhancer of split 5 (Drosophila) 714 +/- 104 558 +/- 31 840 +/- 53 603 +/- 61 -0.201518408 -0.017729321 0.181154127 0.010706611 HMGCS2 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) 244 +/- 51 182 +/- 18 480 +/- 52 240 +/- 77 -0.292635533 -0.035266431 0.585195934 0.034101857 ITCH itchy homolog E3 ubiquitin protein ligase (mouse) 198 +/- 29 78 +/- 10 178 +/- 21 75 +/- 12 -0.007101125 -0.002594673 0.06165396 0.02277359 KBTBD3 kelch repeat and BTB (POZ) domain containing 3 245 +/- 17 231 +/- 14 224 +/- 11 186 +/- 9 -0.559314414 -0.027498256 0.880329743 0.044575316 KCNA5 potassium voltage-gated channel, shaker-related subfamily, member 5 160 +/- 33 140 +/- 13 180 +/- 11 124 +/- 7 -0.581877056 -0.00230911 0.975166744 0.032232243 KCNK2 potassium channel, subfamily K, member 2 130 +/- 38 100 +/- 18 109 +/- 11 75 +/- 3 -0.483792884 -0.022770232 0.25719599 0.029869355 KIAA1559 mouse zinc finger protein 14-like 130 +/- 17 122 +/- 12 158 +/- 9 116 +/- 6 -0.731878664 -0.003437313 0.596634941 0.016257438 KIF21A kinesin family member 21A 3821 +/- 217 2757 +/- 229 4422 +/- 302 3119 +/- 95 -0.007042682 -0.006370346 0.063541337 0.033469949 KIF21B kinesin family member 21B 3015 +/- 342 2089 +/- 271 2816 +/- 158 2164 +/- 78 -0.060922803 -0.007346141 0.07265647 0.021005535 KIF26B kinesin family member 26B 192 +/- 20 165 +/- 9 221 +/- 16 158 +/- 13 -0.24778855 -0.012319096 0.100888922 0.042473042 KLHL21 kelch-like 21 (Drosophila) 1952 +/- 122 1902 +/- 103 1702 +/- 32 1533 +/- 45 -0.762969473 -0.016512575 0.790832399 0.008625901

S4- MSI correlated and non-correlated ARGs.xls Page 20 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG Kua ubiquitin-conjugating enzyme variant Kua 968 +/- 77 876 +/- 70 858 +/- 38 702 +/- 42 -0.400916153 -0.022811901 0.675509359 0.020384394 LCN6 lipocalin 6 288 +/- 28 285 +/- 19 344 +/- 14 251 +/- 22 -0.929214387 -0.009156691 0.561889378 0.041143436 LENG9 leukocyte receptor cluster (LRC) member 9 234 +/- 30 234 +/- 18 289 +/- 10 218 +/- 13 0.994327856 -0.003088573 0.868656502 0.014513834 LRFN5 Leucine rich repeat and fibronectin type III domain containing 5 378 +/- 17 288 +/- 38 451 +/- 36 256 +/- 16 -0.066662424 -0.001745997 0.041556395 0.032559101 LRRC37B leucine rich repeat containing 37B 85 +/- 12 114 +/- 6 139 +/- 7 97 +/- 7 0.06336265 -0.002602785 0.15913679 0.019178527 LRRN1 leucine rich repeat neuronal 1 2089 +/- 141 1602 +/- 137 2532 +/- 162 1964 +/- 83 -0.032954458 -0.015981412 0.054387151 0.048776003 LRRN3 leucine rich repeat neuronal 3 1738 +/- 84 1547 +/- 86 1796 +/- 57 1379 +/- 50 -0.142278448 -0.000374981 0.325883982 0.007219934 MAP3K3 mitogen-activated protein kinase kinase kinase 3 365 +/- 26 320 +/- 15 396 +/- 23 317 +/- 17 -0.17909429 -0.021845319 0.13885626 0.045208597 MEIS3P1 Meis1 homolog 3 (mouse) pseudogene 1 175 +/- 34 118 +/- 10 239 +/- 25 167 +/- 18 -0.158736701 -0.04382298 0.217601651 0.006329832 MGLL monoglyceride lipase 886 +/- 65 790 +/- 40 920 +/- 52 666 +/- 35 -0.240520361 -0.003362636 0.167516032 0.01555855 MLL5 myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila) 1973 +/- 306 931 +/- 182 2063 +/- 365 760 +/- 108 -0.018738458 -0.014618871 0.060188689 0.038757976 MTHFD2L methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like 641 +/- 53 440 +/- 16 569 +/- 21 415 +/- 23 -0.01097374 -0.000813626 0.082986138 0.023699547 NDUFC1 NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa 6197 +/- 241 5433 +/- 247 6237 +/- 149 5646 +/- 145 -0.051220242 -0.019430544 0.032925781 0.032652068 NDUFS2 NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) 2696 +/- 97 2386 +/- 111 2750 +/- 69 2421 +/- 74 -0.062112303 -0.010541112 0.022389632 0.012268038 NUDT4 nudix (nucleoside diphosphate linked moiety X)-type motif 4 341 +/- 23 281 +/- 33 505 +/- 25 364 +/- 47 -0.167954878 -0.036215189 0.209635105 0.049616983 NUPL2 nucleoporin like 2 194 +/- 5 182 +/- 13 206 +/- 12 164 +/- 7 -0.454049599 -0.015258117 0.76688643 0.007473306 OR2C1 olfactory receptor, family 2, subfamily C, member 1 141 +/- 10 140 +/- 11 189 +/- 16 125 +/- 15 -0.916288725 -0.01456367 0.843650052 0.024324144 OSBPL11 oxysterol binding protein-like 11 1537 +/- 69 1374 +/- 41 1376 +/- 35 1266 +/- 31 -0.075652466 -0.044284612 0.069451149 0.014633575 P2RY12 purinergic receptor P2Y, G-protein coupled, 12 1248 +/- 88 1177 +/- 79 1077 +/- 48 848 +/- 66 -0.565026354 -0.023701167 0.997265738 0.03888809 PCDH1 protocadherin 1 (cadherin-like 1) 178 +/- 17 162 +/- 12 240 +/- 16 186 +/- 15 -0.439022235 -0.039922414 0.152628129 0.025625709 PCDHB14 protocadherin beta 14 224 +/- 13 229 +/- 9 342 +/- 16 269 +/- 6 0.732190254 -0.004446946 0.213537685 0.033346171 PCID2 PCI domain containing 2 422 +/- 26 371 +/- 24 406 +/- 11 289 +/- 11 -0.183262368 -4.09472E-05 0.083305397 0.006369602 PDDC1 Parkinson disease 7 domain containing 1 828 +/- 44 710 +/- 44 853 +/- 30 700 +/- 27 -0.08489584 -0.003948275 0.165316571 0.006908681 PDIA4 protein disulfide isomerase family A, member 4 1106 +/- 38 1038 +/- 64 1073 +/- 53 906 +/- 43 -0.38769338 -0.038212729 0.578389301 0.028982556 PGM2L1 phosphoglucomutase 2-like 1 4422 +/- 615 3043 +/- 130 4291 +/- 170 3223 +/- 103 -0.075239597 -0.000673798 0.037639834 0.003504682 PLEKHA5 Pleckstrin homology domain containing, family A member 5 566 +/- 51 398 +/- 55 952 +/- 56 599 +/- 55 -0.048982668 -0.001487041 0.094460525 0.045348518 PLSCR4 phospholipid scramblase 4 292 +/- 36 311 +/- 25 313 +/- 18 251 +/- 17 0.673124243 -0.032128171 0.34148705 0.020808072 PNCK pregnancy upregulated non-ubiquitously expressed CaM kinase 583 +/- 36 459 +/- 43 769 +/- 37 629 +/- 37 -0.052096118 -0.02638636 0.021366191 0.018621328 PPARG Peroxisome proliferative activated receptor, gamma 118 +/- 5 118 +/- 16 156 +/- 13 117 +/- 8 -0.977405063 -0.035008151 0.839520124 0.022127059 PPP2R5C protein phosphatase 2, regulatory subunit B (B56), gamma isoform 1380 +/- 94 967 +/- 75 1220 +/- 33 860 +/- 51 -0.006726147 -0.000552639 0.108054221 0.023206134 PRPF39 PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae) 398 +/- 54 361 +/- 33 488 +/- 33 340 +/- 41 -0.571760018 -0.023066137 0.945513991 0.017866758 PTMS parathymosin 564 +/- 27 451 +/- 59 772 +/- 43 611 +/- 28 -0.124459707 -0.013133005 0.103638104 0.020026595 RAB21 RAB21, member RAS oncogene family 670 +/- 41 691 +/- 68 764 +/- 30 643 +/- 32 0.800378023 -0.022052128 0.294700283 0.011332116 RAPGEF1 Rap guanine nucleotide exchange factor (GEF) 1 616 +/- 50 396 +/- 101 775 +/- 26 416 +/- 82 -0.091584319 -0.009500164 0.072240963 0.003969325 RASGRF1 Ras protein-specific guanine nucleotide-releasing factor 1 125 +/- 16 76 +/- 11 145 +/- 12 104 +/- 10 -0.031471125 -0.025192212 0.075248629 0.025369786 RBM8A RNA binding motif protein 8A 1042 +/- 66 948 +/- 45 1249 +/- 91 986 +/- 63 -0.27077218 -0.0428241 0.208288057 0.032911939 RCOR3 REST corepressor 3 986 +/- 96 990 +/- 27 1072 +/- 16 989 +/- 23 0.97249016 -0.020621067 0.714510185 0.009122733 RECK reversion-inducing-cysteine-rich protein with kazal motifs 328 +/- 29 364 +/- 13 406 +/- 21 327 +/- 12 0.303705244 -0.011967506 0.384147858 0.044990116 RFT1 RFT1 homolog (S. cerevisiae) 144 +/- 6 123 +/- 7 159 +/- 9 123 +/- 5 -0.053411546 -0.009489794 0.11623316 0.018399732 RNF187 ring finger protein 187 4466 +/- 174 4113 +/- 168 4156 +/- 120 3630 +/- 66 -0.175608613 -0.005496012 0.03837654 0.030964401 RNGTT RNA guanylyltransferase and 5'-phosphatase 1248 +/- 42 1197 +/- 32 1228 +/- 26 1129 +/- 20 -0.358665991 -0.015550294 0.217333307 0.025078609 RPL15 Ribosomal protein L15 201 +/- 25 146 +/- 14 188 +/- 13 126 +/- 10 -0.091943479 -0.005182342 0.136520664 0.008485474 SAMD1 sterile alpha motif domain containing 1 363 +/- 22 312 +/- 35 561 +/- 23 439 +/- 31 -0.249772239 -0.01380424 0.215017343 0.015394941 SCYE1 small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) 1313 +/- 43 1283 +/- 31 1364 +/- 27 1259 +/- 16 -0.585045499 -0.009864912 0.670894991 0.032153635

S4- MSI correlated and non-correlated ARGs.xls Page 21 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG SDHC succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa 1763 +/- 91 1634 +/- 45 1610 +/- 52 1461 +/- 35 -0.24292388 -0.043666702 0.274680792 0.025762003 SEC61A1 Sec61 alpha 1 subunit (S. cerevisiae) 320 +/- 43 269 +/- 28 351 +/- 21 276 +/- 12 -0.34041934 -0.01454606 0.389987136 0.008682927 SEPT11 Septin 11 2461 +/- 262 1813 +/- 192 2281 +/- 37 1816 +/- 76 -0.076742119 -0.001580166 0.111726301 0.042381075 SF3A3 Splicing factor 3a, subunit 3, 60kDa 3426 +/- 274 2814 +/- 133 2964 +/- 63 2412 +/- 70 -0.082987624 -0.000277917 0.019385298 0.005249531 SFRS7 splicing factor, arginine/serine-rich 7, 35kDa 2011 +/- 79 1722 +/- 78 1994 +/- 57 1707 +/- 55 -0.026627131 -0.005697307 0.056949117 0.011437644 SGEF Src homology 3 domain-containing guanine nucleotide exchange factor 828 +/- 77 772 +/- 31 904 +/- 53 676 +/- 59 -0.51892981 -0.018969929 0.804508372 0.006115158 SIRT6 sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) 255 +/- 17 215 +/- 16 279 +/- 15 203 +/- 7 -0.116655852 -0.002473305 0.053182402 0.001577501 SLC16A14 solute carrier family 16 (monocarboxylic acid transporters), member 14 1075 +/- 41 1004 +/- 75 1195 +/- 34 1038 +/- 52 -0.435971749 -0.039110304 0.488569168 0.029048522 SLC1A4 solute carrier family 1 (glutamate/neutral amino acid transporter), member 4 3466 +/- 213 3097 +/- 143 3093 +/- 119 2554 +/- 111 -0.185180746 -0.00914134 0.09940615 0.019467187 SLC24A5 Solute carrier family 24, member 5 1183 +/- 81 1115 +/- 57 1455 +/- 56 1211 +/- 48 -0.514492674 -0.008948511 0.359875535 0.003277142 SLCO1C1 solute carrier organic anion transporter family, member 1C1 525 +/- 72 446 +/- 45 404 +/- 18 280 +/- 27 -0.380551931 -0.00631492 0.583153647 0.039576717 SOX1 SRY (sex determining region Y)-box 1 375 +/- 39 272 +/- 18 455 +/- 28 336 +/- 23 -0.050160547 -0.010004196 0.137008855 0.008571145 SPA17 sperm autoantigenic protein 17 537 +/- 65 330 +/- 45 443 +/- 23 329 +/- 17 -0.027597301 -0.0033444 0.094748997 0.03123644 SPSB3 splA/ryanodine receptor domain and SOCS box containing 3 474 +/- 49 493 +/- 23 461 +/- 22 396 +/- 17 0.726656483 -0.049145797 0.743619093 0.033496864 SRCAP Snf2-related CBP activator protein 190 +/- 10 242 +/- 8 232 +/- 5 203 +/- 9 0.002225566 -0.025473729 0.012583108 0.036958657 SYNJ2BP Synaptojanin 2 binding protein 752 +/- 43 787 +/- 33 1031 +/- 19 944 +/- 14 0.539353517 -0.005859176 0.184654643 0.012815765 TADA1L transcriptional adaptor 1 (HFI1 homolog, yeast)-like 1627 +/- 54 1500 +/- 42 1705 +/- 66 1508 +/- 31 -0.095763786 -0.030645013 0.385538987 0.048167039 TASP1 taspase, threonine aspartase, 1 270 +/- 19 225 +/- 18 323 +/- 12 254 +/- 14 -0.113117972 -0.005654167 0.068073676 0.049418893 TBCE tubulin-specific chaperone e 1108 +/- 25 1090 +/- 54 1222 +/- 53 1055 +/- 42 -0.76351252 -0.035533135 0.766784152 0.014240059 TCFL5 transcription factor-like 5 (basic helix-loop-helix) 2337 +/- 183 1879 +/- 150 2593 +/- 180 1960 +/- 48 -0.082168467 -0.015833988 0.297969994 0.027309452 TLK1 tousled-like kinase 1 109 +/- 15 104 +/- 11 155 +/- 15 108 +/- 11 -0.81754403 -0.029834017 0.496871032 0.04853314 TMBIM4 transmembrane BAX inhibitor motif containing 4 1977 +/- 143 2256 +/- 151 2223 +/- 85 1815 +/- 95 0.210502634 -0.011327121 0.092182682 0.040954316 TMEM133 transmembrane protein 133 206 +/- 30 171 +/- 21 278 +/- 7 172 +/- 19 -0.363117639 -0.003540306 0.263370834 0.020832555 TMEM50A transmembrane protein 50A 3252 +/- 47 2957 +/- 223 4076 +/- 139 3448 +/- 34 -0.248077685 -0.005474556 0.466680172 0.025314031 TMEM64 Transmembrane protein 64 338 +/- 27 289 +/- 14 377 +/- 21 301 +/- 16 -0.146266474 -0.020924788 0.294943751 0.007329293 TOP1MT topoisomerase (DNA) I, mitochondrial 381 +/- 24 369 +/- 14 378 +/- 17 328 +/- 12 -0.659271799 -0.038397811 0.836729244 0.036522943 TOR3A torsin family 3, member A 298 +/- 17 220 +/- 15 308 +/- 11 206 +/- 13 -0.006223971 -0.000302833 0.067683608 0.001767536 TRA2A transformer-2 alpha 1956 +/- 84 1639 +/- 102 2458 +/- 104 1919 +/- 80 -0.038510101 -0.002776761 0.316371608 0.022918967 TRAF3 TNF receptor-associated factor 3 1143 +/- 89 1002 +/- 66 1473 +/- 46 1224 +/- 60 -0.233135856 -0.011176866 0.193720961 0.047628942 TRAFD1 TRAF-type zinc finger domain containing 1 1185 +/- 77 1021 +/- 41 913 +/- 16 812 +/- 32 -0.098765207 -0.028756386 0.270577645 0.047654431 TRIM2 tripartite motif-containing 2 134 +/- 28 96 +/- 14 147 +/- 12 92 +/- 9 -0.251682401 -0.005939648 0.392827748 0.023035281 TRIM72 tripartite motif-containing 72 123 +/- 14 108 +/- 18 125 +/- 21 67 +/- 8 -0.549455064 -0.039728145 0.856258507 0.046664767 TUBGCP5 tubulin, gamma complex associated protein 5 697 +/- 50 478 +/- 121 647 +/- 36 371 +/- 78 -0.139882002 -0.020016876 0.317303354 0.037428091 UBE2J1 ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) 447 +/- 25 412 +/- 15 587 +/- 24 472 +/- 9 -0.256294667 -0.003565631 0.136324691 0.017011644 UBR1 ubiquitin protein ligase E3 component n-recognin 1 655 +/- 48 599 +/- 38 837 +/- 25 692 +/- 31 -0.387825401 -0.00632613 0.393551965 0.006819937 UCK1 uridine-cytidine kinase 1 1401 +/- 163 1218 +/- 106 1046 +/- 54 836 +/- 69 -0.371207141 -0.042779758 0.477968251 0.032720888 UTP14C UTP14, U3 small nucleolar ribonucleoprotein, homolog C (yeast) 1372 +/- 46 1224 +/- 51 1428 +/- 32 1176 +/- 49 -0.056118622 -0.003609568 0.210345207 0.027890428 VAPA VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa 11082 +/- 381 9798 +/- 151 10132 +/- 197 8992 +/- 317 -0.018084373 -0.018773793 0.114900856 0.004102026 WAS Wiskott-Aldrich syndrome (eczema-thrombocytopenia) 214 +/- 11 177 +/- 8 239 +/- 10 176 +/- 17 -0.021343083 -0.016448585 0.11705994 0.003993011 WDR36 WD repeat domain 36 210 +/- 17 205 +/- 18 260 +/- 9 213 +/- 15 -0.841737844 -0.03709588 0.936279917 0.046643626 XPO5 exportin 5 429 +/- 36 342 +/- 18 461 +/- 21 383 +/- 24 -0.066394218 -0.040657039 0.216975537 0.026211771 ZNF142 zinc finger protein 142 (clone pHZ-49) 769 +/- 26 723 +/- 22 760 +/- 22 633 +/- 26 -0.217210881 -0.005034118 0.471469704 0.037953722 ZNF16 zinc finger protein 16 568 +/- 69 420 +/- 40 479 +/- 21 350 +/- 25 -0.099141249 -0.004058845 0.227598526 0.001339729 ZNF304 zinc finger protein 304 417 +/- 19 402 +/- 10 433 +/- 15 375 +/- 12 -0.484142912 -0.014961524 0.39629422 0.004852035

S4- MSI correlated and non-correlated ARGs.xls Page 22 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG ZNF342 zinc finger protein 342 170 +/- 21 134 +/- 18 176 +/- 17 96 +/- 14 -0.232630509 -0.004719813 0.281206822 0.003070648 ZNF436 zinc finger protein 436 845 +/- 77 754 +/- 83 1185 +/- 39 1061 +/- 36 -0.443220789 -0.045876934 0.448246883 0.015784252 ZNF529 zinc finger protein 529 1055 +/- 71 885 +/- 61 1009 +/- 20 833 +/- 33 -0.101266016 -0.00309501 0.113981961 0.002419191 Upregulated with Age in Both Regions, not MSI Correlated ACTC actin, alpha, cardiac muscle 84 +/- 15 206 +/- 35 98 +/- 11 212 +/- 35 0.015564453 0.02973628 0.098010841 0.165795281 ACTR10 actin-related protein 10 homolog (S. cerevisiae) 6172 +/- 108 6822 +/- 248 6301 +/- 210 7510 +/- 229 0.048300301 0.003988195 0.590192181 0.084527105 ADCK4 aarF domain containing kinase 4 430 +/- 24 546 +/- 40 383 +/- 31 503 +/- 38 0.037411918 0.039519137 0.273091814 0.306370996 ALDH3A1 aldehyde dehydrogenase 3 family, memberA1 99 +/- 11 267 +/- 57 115 +/- 22 357 +/- 54 0.031714621 0.008136205 0.140583247 0.129873586 AMY1A amylase, alpha 1A; salivary 217 +/- 46 706 +/- 123 210 +/- 34 692 +/- 108 0.008648671 0.00879563 0.208529984 0.155361317 ANKH ankylosis, progressive homolog (mouse) 2365 +/- 119 2794 +/- 121 2527 +/- 92 2834 +/- 84 0.030335857 0.035777518 0.166489153 0.306504297 ARHGAP1 Rho GTPase activating protein 1 2732 +/- 133 3160 +/- 91 2618 +/- 114 2959 +/- 91 0.026130162 0.045361642 0.13775446 0.424521136 AURKAIP1 aurora kinase A interacting protein 1 1086 +/- 137 1595 +/- 169 1097 +/- 103 1678 +/- 137 0.042673648 0.009991978 0.163010822 0.071334126 CAPN2 calpain 2, (m/II) large subunit 2440 +/- 204 3016 +/- 132 1931 +/- 137 2492 +/- 70 0.043050555 0.007570247 0.056262271 0.076747121 CBX3 chromobox homolog 3 (HP1 gamma homolog, Drosophila) 2091 +/- 138 2460 +/- 88 2478 +/- 74 2807 +/- 90 0.052848432 0.02160915 0.09765007 0.057418071 CCT3 chaperonin containing TCP1, subunit 3 (gamma) 2068 +/- 138 2872 +/- 102 1999 +/- 97 2727 +/- 72 0.001085379 0.000217763 0.075896541 0.061884585 CD81 CD81 molecule 13811 +/- 551 15425 +/- 255 12732 +/- 477 14927 +/- 743 0.032375137 0.041802505 0.194433538 0.42501107 COPS7A COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis) 2132 +/- 50 2760 +/- 207 2135 +/- 183 2910 +/- 174 0.027680484 0.01351365 0.352985886 0.252014955 COQ3 coenzyme Q3 homolog, methyltransferase (S. cerevisiae) 962 +/- 18 1137 +/- 65 967 +/- 22 1098 +/- 25 0.043000068 0.00382441 0.334787333 0.133082872 COX5B cytochrome c oxidase subunit Vb 6825 +/- 788 9221 +/- 528 4736 +/- 885 7292 +/- 122 0.033108929 0.03387958 0.066557107 0.06557043 COX6C cytochrome c oxidase subunit VIc 7080 +/- 300 8976 +/- 497 6419 +/- 237 8142 +/- 260 0.011021066 0.0009592 0.204294097 0.061635335 CRNKL1 Crn, crooked neck-like 1 (Drosophila) 267 +/- 89 512 +/- 56 270 +/- 68 595 +/- 45 0.046427355 0.003682882 0.102437924 0.059562553 CYCS cytochrome c, somatic 1114 +/- 69 1523 +/- 105 1124 +/- 93 1485 +/- 52 0.010341606 0.010190902 0.183603931 0.132190411 DOCK4 dedicator of cytokinesis 4 83 +/- 24 256 +/- 47 145 +/- 31 379 +/- 38 0.011861311 0.00134105 0.068950788 0.102620985 ENO1 enolase 1, (alpha) 7846 +/- 333 9198 +/- 336 6480 +/- 135 7880 +/- 253 0.016967227 0.002519542 0.07839016 0.056871124 FARP1 FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) 387 +/- 68 1140 +/- 179 336 +/- 59 910 +/- 114 0.006669617 0.004168685 0.139806747 0.091282321 GINS1 GINS complex subunit 1 (Psf1 homolog) 2077 +/- 122 2623 +/- 181 2094 +/- 32 2596 +/- 160 0.034320522 0.033856266 0.367407227 0.345335418 GRHPR glyoxylate reductase/hydroxypyruvate reductase 1359 +/- 124 1733 +/- 104 1291 +/- 74 1730 +/- 83 0.044182681 0.003678999 0.22865394 0.068657893 HSPB8 heat shock 22kDa protein 8 2202 +/- 213 3231 +/- 299 2037 +/- 225 2797 +/- 223 0.020507528 0.040315812 0.080176878 0.384594436 IBRDC3 IBR domain containing 3 179 +/- 43 292 +/- 22 217 +/- 41 344 +/- 26 0.047629109 0.029838298 0.219191824 0.299550357 KIF3A kinesin family member 3A 6022 +/- 351 7526 +/- 490 5756 +/- 353 7952 +/- 415 0.033884807 0.003445953 0.447769441 0.104240769 KIF5A kinesin family member 5A 2488 +/- 627 4967 +/- 527 2216 +/- 519 3839 +/- 250 0.013155403 0.025515348 0.069802094 0.168159715 KRR1 KRR1, small subunit (SSU) processome component, homolog (yeast) 143 +/- 22 372 +/- 77 168 +/- 37 387 +/- 79 0.030300603 0.048322189 0.282459063 0.327733661 MAP3K13 Mitogen-activated protein kinase kinase kinase 13 655 +/- 36 806 +/- 40 539 +/- 37 736 +/- 36 0.019233825 0.004277603 0.193007684 0.08807373 METTL5 methyltransferase like 5 537 +/- 35 732 +/- 38 613 +/- 18 740 +/- 26 0.003613606 0.004300567 0.06472819 0.139529211 MYLK myosin, light polypeptide kinase 2425 +/- 309 3340 +/- 241 1446 +/- 132 2443 +/- 131 0.043082979 0.000477748 0.11663479 0.069364667 NFX1 nuclear transcription factor, X-box binding 1 115 +/- 15 162 +/- 12 126 +/- 3 169 +/- 16 0.039838428 0.047698285 0.119763606 0.078436117 NUDT5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 884 +/- 75 1105 +/- 67 672 +/- 11 954 +/- 63 0.053390579 0.010161315 0.094210476 0.12241466 PLCH2 phospholipase C, eta 2 370 +/- 51 502 +/- 14 513 +/- 63 801 +/- 59 0.049362678 0.008956677 0.067947152 0.087632183 PLEKHA6 pleckstrin homology domain containing, family A member 6 566 +/- 46 738 +/- 53 585 +/- 29 732 +/- 40 0.034289835 0.018921166 0.162664897 0.080752046 PMS2 PMS2 postmeiotic segregation increased 2 (S. cerevisiae) 429 +/- 49 1254 +/- 258 530 +/- 58 1843 +/- 341 0.023149503 0.017204806 0.200908195 0.119468895 POLD2 Polymerase (DNA directed), delta 2, regulatory subunit 50kDa 88 +/- 13 142 +/- 12 136 +/- 20 197 +/- 12 0.01183106 0.028238118 0.079666315 0.256120179 PTP4A3 protein tyrosine phosphatase type IVA, member 3 107 +/- 7 143 +/- 10 103 +/- 8 139 +/- 10 0.013215792 0.023195861 0.197196097 0.071717067 RABL4 RAB, member of RAS oncogene family-like 4 740 +/- 95 1128 +/- 110 549 +/- 56 835 +/- 106 0.023895769 0.054057633 0.076826758 0.321291487 RANBP5 RAN binding protein 5 936 +/- 51 1233 +/- 94 1110 +/- 54 1359 +/- 44 0.025319009 0.005955687 0.189239579 0.186509537

S4- MSI correlated and non-correlated ARGs.xls Page 23 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG S100A6 S100 calcium binding protein A6 (calcyclin) 923 +/- 64 1156 +/- 72 682 +/- 81 1085 +/- 58 0.035962432 0.003149417 0.220246563 0.1084609 SERPINB8 serpin peptidase inhibitor, clade B (ovalbumin), member 8 103 +/- 14 142 +/- 10 101 +/- 7 125 +/- 8 0.0451369 0.047732079 0.210402796 0.567889163 SLC12A9 solute carrier family 12 (potassium/chloride transporters), member 9 1093 +/- 101 1413 +/- 80 1023 +/- 47 1257 +/- 43 0.033133089 0.005050841 0.144496165 0.152042307 SSU72 SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae) 2015 +/- 93 2625 +/- 115 1716 +/- 93 2050 +/- 54 0.002266345 0.015168408 0.111942881 0.067295625 STOM stomatin 2053 +/- 253 2990 +/- 261 1953 +/- 163 2755 +/- 171 0.027515679 0.008200839 0.063237653 0.157507683 TATDN3 TatD DNase domain containing 3 116 +/- 9 169 +/- 11 138 +/- 16 192 +/- 10 0.0041436 0.02164698 0.07241459 0.055340933 TCEAL4 transcription elongation factor A (SII)-like 4 8509 +/- 229 10384 +/- 384 8133 +/- 200 10329 +/- 553 0.00289964 0.013325761 0.097909518 0.138275737 TMEM106C transmembrane protein 106C 1970 +/- 98 2345 +/- 128 1940 +/- 209 2804 +/- 219 0.043413259 0.019261834 0.312849989 0.22733026 TMEM11 transmembrane protein 11 845 +/- 33 981 +/- 25 769 +/- 31 945 +/- 37 0.00892987 0.006195045 0.139897829 0.106812496 TRUB2 TruB pseudouridine (psi) synthase homolog 2 (E. coli) 855 +/- 93 1100 +/- 56 795 +/- 42 1011 +/- 37 0.052142304 0.003952869 0.128420178 0.100419906 USP31 ubiquitin specific peptidase 31 360 +/- 19 436 +/- 27 499 +/- 36 687 +/- 45 0.045849929 0.011486388 0.133505683 0.121915743 XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku a 1545 +/- 90 1864 +/- 59 1424 +/- 33 1774 +/- 97 0.016491456 0.01936134 0.248647388 0.118649987 ZNF131 Zinc finger protein 131 (clone pHZ-10) 124 +/- 18 238 +/- 29 160 +/- 22 267 +/- 25 0.010052177 0.011395349 0.126156615 0.066808291 ZNF25 zinc finger protein 25 1239 +/- 41 1519 +/- 51 1305 +/- 50 1653 +/- 73 0.001854661 0.005249539 0.085885628 0.065991508 ZNF45 zinc finger protein 45 279 +/- 18 336 +/- 17 332 +/- 22 400 +/- 18 0.049501338 0.045353643 0.241044901 0.225942957 ZRANB1 zinc finger, RAN-binding domain containing 1 849 +/- 44 1040 +/- 45 887 +/- 57 1073 +/- 52 0.012514974 0.039302018 0.25207767 0.26501462 Downregulated with Age in Both Regions, not MSI Correlated ATP8B2 ATPase, Class I, type 8B, member 2 1260 +/- 36 1102 +/- 43 1508 +/- 74 1279 +/- 39 -0.018717994 -0.027391031 0.071600827 0.129279316 BRD2 bromodomain containing 2 683 +/- 52 469 +/- 53 945 +/- 41 572 +/- 72 -0.016524852 -0.003473977 0.280047378 0.097054419 CACNA1A calcium channel, voltage-dependent, P/Q type, alpha 1A subunit 961 +/- 89 664 +/- 44 1617 +/- 110 993 +/- 119 -0.018644654 -0.00419325 0.07476412 0.121622277 CKAP1 cytoskeleton associated protein 1 1486 +/- 142 1105 +/- 70 1406 +/- 119 962 +/- 130 -0.04564535 -0.033776237 0.283899216 0.20179708 CLK4 CDC-like kinase 4 2680 +/- 52 2262 +/- 87 2336 +/- 119 1847 +/- 72 -0.003236721 -0.007870411 0.089082266 0.093570671 CREBBP CREB binding protein (Rubinstein-Taybi syndrome) 1537 +/- 76 1340 +/- 29 1670 +/- 25 1368 +/- 90 -0.047722227 -0.026138044 0.218216685 0.238085085 DCTN4 dynactin 4 (p62) 2167 +/- 114 1786 +/- 115 2057 +/- 72 1769 +/- 87 -0.03959171 -0.033853445 0.439314547 0.327635314 DFFA DNA fragmentation factor, 45kDa, alpha polypeptide 632 +/- 34 537 +/- 27 694 +/- 27 593 +/- 28 -0.054305353 -0.031207415 0.19627277 0.297678699 DKFZP779L1PRO0845 1349 +/- 59 1055 +/- 62 1202 +/- 31 955 +/- 46 -0.006254246 -0.002851053 0.117590109 0.084762499 GTF2IRD2 GTF2I repeat domain containing 2 310 +/- 18 224 +/- 27 365 +/- 33 210 +/- 55 -0.028055011 -0.047209612 0.482695937 0.339189571 KCNK17 potassium channel, subfamily K, member 17 175 +/- 17 111 +/- 10 253 +/- 20 177 +/- 20 -0.011001664 -0.023816161 0.072727066 0.084128799 KLF13 Kruppel-like factor 13 1790 +/- 60 1158 +/- 151 2000 +/- 133 1313 +/- 196 -0.006850529 -0.022009748 0.148842536 0.386259152 LRP11 low density lipoprotein receptor-related protein 11 4775 +/- 167 4237 +/- 116 4754 +/- 143 4135 +/- 226 -0.026691143 -0.054358382 0.213604269 0.477677495 LTBP1 latent transforming growth factor beta binding protein 1 361 +/- 17 236 +/- 20 447 +/- 38 218 +/- 28 -0.00071457 -0.000958711 0.098246847 0.0743978 MAP1LC3A microtubule-associated protein 1 light chain 3 alpha 3967 +/- 274 2363 +/- 245 4800 +/- 253 2961 +/- 404 -0.00144192 -0.00636558 0.059834641 0.163614208 MARK2 MAP/microtubule affinity-regulating kinase 2 823 +/- 33 702 +/- 30 941 +/- 40 765 +/- 25 -0.023035541 -0.005706683 0.135183632 0.132393049 MAX MYC associated factor X 1031 +/- 87 779 +/- 39 1103 +/- 54 911 +/- 39 -0.034213781 -0.019564295 0.067448525 0.207610623 MOBKL2B MOB1, Mps One Binder kinase activator-like 2B (yeast) 138 +/- 10 100 +/- 11 127 +/- 7 86 +/- 4 -0.028521632 -0.001403645 0.062958159 0.055919928 OSBPL6 oxysterol binding protein-like 6 2412 +/- 155 1905 +/- 45 2468 +/- 135 1963 +/- 75 -0.02064243 -0.012145114 0.093437471 0.227481628 PABPC5 poly(A) binding protein, cytoplasmic 5 153 +/- 5 103 +/- 10 160 +/- 15 110 +/- 8 -0.003193097 -0.019984793 0.089753987 0.125170727 PNLDC1 poly(A)-specific ribonuclease (PARN)-like domain containing 1 203 +/- 62 42 +/- 1 212 +/- 57 60 +/- 6 -0.047861142 -0.044611804 0.195803754 0.13121375 PROCA1 proline-rich cyclin A1-interacting protein 188 +/- 17 132 +/- 6 195 +/- 11 162 +/- 7 -0.01904541 -0.03118106 0.104932479 0.258450261 PTBP2 polypyrimidine tract binding protein 2 2307 +/- 103 1782 +/- 161 2298 +/- 39 1811 +/- 152 -0.02381853 -0.030830317 0.438613881 0.216102612 PUM2 pumilio homolog 2 (Drosophila) 2633 +/- 77 1935 +/- 111 2753 +/- 131 1997 +/- 141 -0.000604458 -0.003648263 0.05562315 0.081645211 RIT2 Ras-like without CAAX 2 609 +/- 31 437 +/- 55 775 +/- 62 568 +/- 48 -0.025575445 -0.027875631 0.077862913 0.062894532 RNF10 ring finger protein 10 3235 +/- 192 2478 +/- 121 3155 +/- 83 2677 +/- 130 -0.009517874 -0.017449864 0.082708791 0.409322341 RNF40 ring finger protein 40 697 +/- 31 616 +/- 10 876 +/- 27 750 +/- 31 -0.043586854 -0.014864958 0.340172388 0.108042495

S4- MSI correlated and non-correlated ARGs.xls Page 24 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG RPS12 ribosomal protein S12 4329 +/- 211 3150 +/- 239 3422 +/- 186 2508 +/- 191 -0.004218543 -0.00763633 0.093213947 0.150396701 SEC14L1 SEC14-like 1 (S. cerevisiae) 4771 +/- 153 4085 +/- 221 5018 +/- 284 3990 +/- 299 -0.031449238 -0.03470786 0.459700401 0.261595307 SETD6 SET domain containing 6 357 +/- 14 262 +/- 22 362 +/- 8 285 +/- 23 -0.006155276 -0.024760878 0.071339234 0.157550143 SGPL1 sphingosine-1-phosphate lyase 1 613 +/- 28 522 +/- 26 640 +/- 24 524 +/- 12 -0.040205869 -0.002866218 0.231950816 0.057885563 SMU1 Smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) 1021 +/- 44 845 +/- 49 1000 +/- 21 892 +/- 31 -0.024100861 -0.021557898 0.061954055 0.167536525 SORBS2 Sorbin and SH3 domain containing 2 432 +/- 19 353 +/- 14 405 +/- 14 294 +/- 41 -0.007594933 -0.049321265 0.078440767 0.364208937 SRrp35 serine-arginine repressor protein (35 kDa) 851 +/- 61 650 +/- 57 860 +/- 61 692 +/- 42 -0.036873369 -0.05114665 0.063738218 0.19318906 SSB Sjogren syndrome antigen B (autoantigen La) 2114 +/- 118 1777 +/- 99 2359 +/- 70 1882 +/- 138 -0.054697646 -0.021627807 0.061261408 0.162029076 SUB1 SUB1 homolog (S. cerevisiae) 198 +/- 12 153 +/- 13 233 +/- 7 187 +/- 11 -0.029380853 -0.009334405 0.070572835 0.200849812 SURF4 surfeit 4 844 +/- 29 730 +/- 19 751 +/- 33 661 +/- 21 -0.010483129 -0.048622922 0.102185092 0.242072965 TBC1D14 TBC1 domain family, member 14 2125 +/- 84 1815 +/- 92 1795 +/- 52 1639 +/- 43 -0.031696434 -0.047244724 0.222267241 0.2088684 TMEM132B transmembrane protein 132B 1274 +/- 123 930 +/- 71 1051 +/- 67 730 +/- 54 -0.041704541 -0.004741841 0.240402673 0.156696458 TTC19 tetratricopeptide repeat domain 19 2924 +/- 101 1559 +/- 359 3189 +/- 138 1757 +/- 528 -0.011318191 -0.051446777 0.065680723 0.168026491 USP15 ubiquitin specific peptidase 15 2158 +/- 53 1639 +/- 107 2009 +/- 51 1697 +/- 92 -0.003134811 -0.023252573 0.058322123 0.324435591 YTHDF2 YTH domain family, member 2 2059 +/- 97 1665 +/- 58 2492 +/- 66 2178 +/- 98 -0.007866082 -0.031414013 0.112830832 0.432848963 ZBTB24 zinc finger and BTB domain containing 24 626 +/- 63 454 +/- 23 608 +/- 38 435 +/- 38 -0.039583377 -0.01011329 0.187721069 0.150985666 ZNF74 zinc finger protein 74 147 +/- 15 91 +/- 13 170 +/- 12 113 +/- 14 -0.018936105 -0.016370007 0.112743819 0.206491307 ZNRD1 zinc ribbon domain containing 1 926 +/- 41 625 +/- 37 853 +/- 26 720 +/- 30 -0.000268689 -0.009907701 0.05962573 0.099213787 ZXDB zinc finger, X-linked, duplicated B 667 +/- 61 496 +/- 39 665 +/- 46 479 +/- 61 -0.044558801 -0.041047339 0.260164736 0.248674131 Upregulated with Age in CA, not MSI Correlated ACSS1 acyl-CoA synthetase short-chain family member 1 229 +/- 21 311 +/- 28 196 +/- 16 228 +/- 23 0.044219773 0.295382048 0.092173026 0.41519286 ACTC actin, alpha, cardiac muscle 84 +/- 15 206 +/- 35 98 +/- 11 212 +/- 35 0.015564453 0.02973628 0.098010841 0.165795281 ACTR10 actin-related protein 10 homolog (S. cerevisiae) 6172 +/- 108 6822 +/- 248 6301 +/- 210 7510 +/- 229 0.048300301 0.003988195 0.590192181 0.084527105 ADAM15 ADAM metallopeptidase domain 15 (metargidin) 1022 +/- 24 1299 +/- 94 1046 +/- 106 1001 +/- 50 0.030854629 -0.71105561 0.141754929 0.762483649 ADCK4 aarF domain containing kinase 4 430 +/- 24 546 +/- 40 383 +/- 31 503 +/- 38 0.037411918 0.039519137 0.273091814 0.306370996 AKAP2 A kinase (PRKA) anchor protein 2 119 +/- 16 187 +/- 25 136 +/- 11 180 +/- 30 0.048876962 0.230582964 0.104341568 0.703394196 ALDH3A1 aldehyde dehydrogenase 3 family, memberA1 99 +/- 11 267 +/- 57 115 +/- 22 357 +/- 54 0.031714621 0.008136205 0.140583247 0.129873586 AMY1A amylase, alpha 1A; salivary 217 +/- 46 706 +/- 123 210 +/- 34 692 +/- 108 0.008648671 0.00879563 0.208529984 0.155361317 ANKH ankylosis, progressive homolog (mouse) 2365 +/- 119 2794 +/- 121 2527 +/- 92 2834 +/- 84 0.030335857 0.035777518 0.166489153 0.306504297 ARHGAP1 Rho GTPase activating protein 1 2732 +/- 133 3160 +/- 91 2618 +/- 114 2959 +/- 91 0.026130162 0.045361642 0.13775446 0.424521136 ARID1B AT rich interactive domain 1B (SWI1-like) 517 +/- 50 742 +/- 58 815 +/- 107 980 +/- 67 0.015336606 0.224649318 0.1181921 0.40132601 ARIH1 Ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) 340 +/- 7 374 +/- 9 372 +/- 17 385 +/- 27 0.012077729 0.684704776 0.077969394 0.223103894 ARSD arylsulfatase D 461 +/- 56 623 +/- 41 464 +/- 64 612 +/- 50 0.043786951 0.101284212 0.102416826 0.156049993 ATAD2 ATPase family, AAA domain containing 2 97 +/- 15 148 +/- 7 117 +/- 7 127 +/- 8 0.018121133 0.411450294 0.086430788 0.285110489 ATXN7L1 ataxin 7-like 1 462 +/- 25 555 +/- 22 612 +/- 31 626 +/- 34 0.019018807 0.771269241 0.08401584 0.431720954 AURKAIP1 aurora kinase A interacting protein 1 1086 +/- 137 1595 +/- 169 1097 +/- 103 1678 +/- 137 0.042673648 0.009991978 0.163010822 0.071334126 AXL AXL receptor tyrosine kinase 951 +/- 51 1108 +/- 50 906 +/- 51 754 +/- 67 0.054106048 -0.110760834 0.20280057 0.01696212 BAG1 BCL2-associated athanogene 642 +/- 54 817 +/- 54 829 +/- 57 905 +/- 31 0.045918545 0.271392547 0.254928509 0.394042998 BTG4 B-cell translocation gene 4 106 +/- 9 193 +/- 31 105 +/- 8 196 +/- 11 0.035256012 0.000213402 0.093942226 0.005027946 BTN3A1 butyrophilin, subfamily 3, member A1 194 +/- 23 323 +/- 36 209 +/- 19 260 +/- 29 0.016382779 0.183901688 0.057796542 0.148192434 CAPN2 calpain 2, (m/II) large subunit 2440 +/- 204 3016 +/- 132 1931 +/- 137 2492 +/- 70 0.043050555 0.007570247 0.056262271 0.076747121 CBX3 chromobox homolog 3 (HP1 gamma homolog, Drosophila) 2091 +/- 138 2460 +/- 88 2478 +/- 74 2807 +/- 90 0.052848432 0.02160915 0.09765007 0.057418071 CCT3 chaperonin containing TCP1, subunit 3 (gamma) 2068 +/- 138 2872 +/- 102 1999 +/- 97 2727 +/- 72 0.001085379 0.000217763 0.075896541 0.061884585 CD2BP2 CD2 (cytoplasmic tail) binding protein 2 434 +/- 27 533 +/- 24 382 +/- 9 508 +/- 28 0.022462219 0.007719463 0.191814082 0.022361307

S4- MSI correlated and non-correlated ARGs.xls Page 25 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG CD81 CD81 molecule 13811 +/- 551 15425 +/- 255 12732 +/- 477 14927 +/- 743 0.032375137 0.041802505 0.194433538 0.42501107 CDC16 CDC16 cell division cycle 16 homolog (S. cerevisiae) 1071 +/- 33 1180 +/- 23 991 +/- 39 1063 +/- 17 0.024866349 0.134430028 0.13100448 0.324503036 CDC25A cell division cycle 25A 235 +/- 35 330 +/- 18 224 +/- 25 264 +/- 25 0.041930863 0.285905226 0.206982299 0.662240565 CHCHD7 coiled-coil-helix-coiled-coil-helix domain containing 7 1443 +/- 50 1660 +/- 71 1485 +/- 74 1658 +/- 34 0.034158169 0.071555797 0.122505083 0.117042564 CHL1 cell adhesion molecule with homology to L1CAM (close homolog of L1) 5666 +/- 319 6673 +/- 332 5043 +/- 360 5085 +/- 214 0.053767932 0.922983686 0.26905998 0.966686244 CLCC1 chloride channel CLIC-like 1 332 +/- 29 441 +/- 25 310 +/- 14 395 +/- 5 0.017994612 0.001144694 0.066524997 0.011344007 CLN3 ceroid-lipofuscinosis, neuronal 3, juvenile (Batten, Spielmeyer-Vogt disease) 69 +/- 19 166 +/- 24 72 +/- 18 167 +/- 36 0.010473862 0.059108282 0.162109144 0.4560878 COL8A1 Collagen, type VIII, alpha 1 97 +/- 13 150 +/- 14 69 +/- 14 113 +/- 15 0.016482587 0.061623491 0.055660334 0.005594877 COPS7A COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis) 2132 +/- 50 2760 +/- 207 2135 +/- 183 2910 +/- 174 0.027680484 0.01351365 0.352985886 0.252014955 COQ3 coenzyme Q3 homolog, methyltransferase (S. cerevisiae) 962 +/- 18 1137 +/- 65 967 +/- 22 1098 +/- 25 0.043000068 0.00382441 0.334787333 0.133082872 COX5B cytochrome c oxidase subunit Vb 6825 +/- 788 9221 +/- 528 4736 +/- 885 7292 +/- 122 0.033108929 0.03387958 0.066557107 0.06557043 COX6C cytochrome c oxidase subunit VIc 7080 +/- 300 8976 +/- 497 6419 +/- 237 8142 +/- 260 0.011021066 0.0009592 0.204294097 0.061635335 CRAMP1L Crm, cramped-like (Drosophila) 799 +/- 22 1083 +/- 81 759 +/- 43 853 +/- 75 0.015800607 0.319565521 0.109389 0.816449511 CRNKL1 Crn, crooked neck-like 1 (Drosophila) 267 +/- 89 512 +/- 56 270 +/- 68 595 +/- 45 0.046427355 0.003682882 0.102437924 0.059562553 CUGBP2 CUG triplet repeat, RNA binding protein 2 1263 +/- 229 2289 +/- 319 1772 +/- 270 3161 +/- 347 0.028023032 0.01349226 0.240528635 0.044816364 CXXC4 CXXC finger 4 336 +/- 31 432 +/- 16 266 +/- 21 359 +/- 24 0.025393947 0.017669687 0.098272989 0.053262012 CYCS cytochrome c, somatic 1114 +/- 69 1523 +/- 105 1124 +/- 93 1485 +/- 52 0.010341606 0.010190902 0.183603931 0.132190411 CYP2U1 cytochrome P450, family 2, subfamily U, polypeptide 1 588 +/- 29 675 +/- 23 522 +/- 14 543 +/- 13 0.043277742 0.299250103 0.093998178 0.347334754 DBT dihydrolipoamide branched chain transacylase E2 462 +/- 33 608 +/- 51 504 +/- 30 557 +/- 59 0.039833819 0.456100246 0.162301729 0.969314425 DCTN6 dynactin 6 2538 +/- 127 2919 +/- 63 2668 +/- 126 2992 +/- 196 0.029949775 0.207325665 0.240430018 0.790006856 DDHD1 DDHD domain containing 1 1480 +/- 235 2265 +/- 180 1320 +/- 221 1767 +/- 178 0.025380526 0.151224371 0.129240723 0.571036542 DOCK4 dedicator of cytokinesis 4 83 +/- 24 256 +/- 47 145 +/- 31 379 +/- 38 0.011861311 0.00134105 0.068950788 0.102620985 DPM2 dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit 870 +/- 18 1041 +/- 46 787 +/- 41 847 +/- 24 0.011942914 0.242049888 0.078490281 0.330036341 DTX3L deltex 3-like (Drosophila) 251 +/- 13 312 +/- 18 280 +/- 11 256 +/- 22 0.024096904 -0.357979389 0.09316433 0.31787349 DYNC2H1 dynein, cytoplasmic 2, heavy chain 1 508 +/- 62 728 +/- 72 644 +/- 82 957 +/- 115 0.043945338 0.060176299 0.262692411 0.344315645 EMP3 epithelial membrane protein 3 385 +/- 23 473 +/- 25 311 +/- 21 374 +/- 24 0.029519429 0.081155644 0.095194523 0.797026608 ENO1 enolase 1, (alpha) 7846 +/- 333 9198 +/- 336 6480 +/- 135 7880 +/- 253 0.016967227 0.002519542 0.07839016 0.056871124 ETFDH electron-transferring-flavoprotein dehydrogenase 382 +/- 17 463 +/- 16 356 +/- 16 395 +/- 22 0.006605564 0.190974823 0.109621717 0.459312584 EXOC7 exocyst complex component 7 228 +/- 32 391 +/- 33 285 +/- 12 351 +/- 36 0.004969021 0.144338174 0.076115754 0.211317901 FARP1 FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) 387 +/- 68 1140 +/- 179 336 +/- 59 910 +/- 114 0.006669617 0.004168685 0.139806747 0.091282321 FBXO17 F-box protein 17 244 +/- 20 351 +/- 30 356 +/- 58 418 +/- 44 0.016664726 0.414234698 0.117558843 0.209040806 FBXO38 F-box protein 38 480 +/- 20 548 +/- 23 535 +/- 44 505 +/- 38 0.046100698 -0.619412016 0.30700201 0.439029225 FECH ferrochelatase (protoporphyria) 822 +/- 52 1099 +/- 86 820 +/- 77 1136 +/- 119 0.023949084 0.059793298 0.082661233 0.077081569 FLCN folliculin 85 +/- 4 102 +/- 4 108 +/- 14 107 +/- 6 0.019232872 -0.937966761 0.082039332 0.384839333 FOXO1A forkhead box O1A (rhabdomyosarcoma) 720 +/- 44 1108 +/- 81 1000 +/- 116 1225 +/- 114 0.003120312 0.199945505 0.066269934 0.965805829 FOXO3A Forkhead box O3A 331 +/- 35 512 +/- 68 283 +/- 27 375 +/- 22 0.048674426 0.026860825 0.057457718 0.021827275 GAS7 growth arrest-specific 7 9122 +/- 492 10812 +/- 463 7341 +/- 461 8180 +/- 533 0.031339332 0.265977748 0.166864454 0.19726818 GGTLA4 gamma-glutamyltransferase-like activity 4 155 +/- 2 188 +/- 13 139 +/- 10 162 +/- 8 0.048726219 0.129089576 0.06448907 0.179980162 GINS1 GINS complex subunit 1 (Psf1 homolog) 2077 +/- 122 2623 +/- 181 2094 +/- 32 2596 +/- 160 0.034320522 0.033856266 0.367407227 0.345335418 GIT2 G protein-coupled receptor kinase interactor 2 2774 +/- 76 3131 +/- 101 2423 +/- 103 2775 +/- 131 0.019667627 0.067079427 0.174695151 0.03184067 GOPC golgi associated PDZ and coiled-coil motif containing 462 +/- 34 622 +/- 43 560 +/- 32 730 +/- 26 0.015844249 0.002572784 0.067332845 0.008610876 GPHN Gephyrin 468 +/- 36 658 +/- 46 703 +/- 53 656 +/- 40 0.009339756 -0.495338603 0.070878958 0.286419919 GRHPR glyoxylate reductase/hydroxypyruvate reductase 1359 +/- 124 1733 +/- 104 1291 +/- 74 1730 +/- 83 0.044182681 0.003678999 0.22865394 0.068657893 HBII-276HG HBII-276 host gene 4992 +/- 293 6134 +/- 326 5486 +/- 284 6432 +/- 380 0.02640662 0.082376721 0.079804348 0.216008313

S4- MSI correlated and non-correlated ARGs.xls Page 26 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG HBS1L HBS1-like (S. cerevisiae) 1048 +/- 89 1565 +/- 156 1275 +/- 83 1473 +/- 162 0.020628933 0.319037322 0.086345244 0.918312567 HECTD2 HECT domain containing 2 1579 +/- 126 2196 +/- 109 1410 +/- 84 1984 +/- 71 0.004135061 0.000568656 0.080773087 0.041573814 HLA-DPB1 major histocompatibility complex, class II, DP beta 1 275 +/- 55 485 +/- 42 153 +/- 53 409 +/- 56 0.013814114 0.009354973 0.103180322 0.024830136 HNRPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) 1406 +/- 64 1586 +/- 51 1838 +/- 60 1825 +/- 128 0.054211223 -0.927465461 0.210033818 0.967620935 HNRPL heterogeneous nuclear ribonucleoprotein L 5232 +/- 292 6250 +/- 170 6161 +/- 225 7259 +/- 144 0.016730469 0.003234389 0.14241682 0.023284248 HRH1 Histamine receptor H1 110 +/- 13 169 +/- 16 148 +/- 14 93 +/- 20 0.017877363 -0.05536298 0.074279077 0.026902294 HSDL2 hydroxysteroid dehydrogenase like 2 117 +/- 13 171 +/- 18 115 +/- 10 148 +/- 16 0.036775116 0.129426591 0.085526021 0.004988242 HSP90AB1 heat shock protein 90kDa alpha (cytosolic), class B member 1 11071 +/- 195 13271 +/- 831 9909 +/- 375 10714 +/- 222 0.045089883 0.102338403 0.170591648 0.094284162 HSPB8 heat shock 22kDa protein 8 2202 +/- 213 3231 +/- 299 2037 +/- 225 2797 +/- 223 0.020507528 0.040315812 0.080176878 0.384594436 HSPC176 hematopoietic stem/progenitor cells 176 666 +/- 60 863 +/- 48 612 +/- 77 712 +/- 53 0.029019601 0.316878103 0.261860352 0.275118585 IBRDC3 IBR domain containing 3 179 +/- 43 292 +/- 22 217 +/- 41 344 +/- 26 0.047629109 0.029838298 0.219191824 0.299550357 IFT140 intraflagellar transport 140 homolog (Chlamydomonas) 212 +/- 12 263 +/- 17 244 +/- 23 276 +/- 16 0.037274415 0.272873388 0.071047955 0.342519238 IGSF9 immunoglobulin superfamily, member 9 140 +/- 15 213 +/- 22 159 +/- 9 169 +/- 13 0.020987166 0.530615698 0.06245333 0.17190817 ITGAM integrin, alpha M (complement component 3 receptor 3 subunit) 332 +/- 23 412 +/- 12 323 +/- 26 348 +/- 34 0.016766774 0.584584094 0.08401445 0.55274916 KHDRBS1 KH domain containing, RNA binding, signal transduction associated 1 434 +/- 12 512 +/- 23 424 +/- 28 401 +/- 15 0.016874918 -0.478304553 0.317581896 0.390046933 KIDINS220 Kinase D-interacting substance of 220 kDa 221 +/- 98 520 +/- 82 208 +/- 72 376 +/- 75 0.042029308 0.142024625 0.11277976 0.311622623 KIF3A kinesin family member 3A 6022 +/- 351 7526 +/- 490 5756 +/- 353 7952 +/- 415 0.033884807 0.003445953 0.447769441 0.104240769 KIF5A kinesin family member 5A 2488 +/- 627 4967 +/- 527 2216 +/- 519 3839 +/- 250 0.013155403 0.025515348 0.069802094 0.168159715 KLHL8 kelch-like 8 (Drosophila) 1978 +/- 100 2286 +/- 94 2118 +/- 103 2280 +/- 170 0.049228577 0.44249102 0.231789828 0.597146058 KRR1 KRR1, small subunit (SSU) processome component, homolog (yeast) 143 +/- 22 372 +/- 77 168 +/- 37 387 +/- 79 0.030300603 0.048322189 0.282459063 0.327733661 LIX1L Lix1 homolog (mouse) like 1777 +/- 112 2141 +/- 118 1728 +/- 67 1848 +/- 69 0.049309549 0.244960965 0.08145548 0.44860521 LNPEP leucyl/cystinyl aminopeptidase 2120 +/- 127 2540 +/- 116 2687 +/- 122 2808 +/- 110 0.035157226 0.478573904 0.130928933 0.656830166 LRIG1 leucine-rich repeats and immunoglobulin-like domains 1 2933 +/- 304 3934 +/- 210 2719 +/- 203 3170 +/- 146 0.024241162 0.105786534 0.079044288 0.603652401 LRRC39 leucine rich repeat containing 39 91 +/- 6 115 +/- 9 98 +/- 6 108 +/- 9 0.043875356 0.398741782 0.149198507 0.579822311 LYPLA3 lysophospholipase 3 (lysosomal phospholipase A2) 288 +/- 21 365 +/- 24 331 +/- 23 339 +/- 18 0.03444241 0.774488703 0.162530545 0.896713769 MAP3K13 Mitogen-activated protein kinase kinase kinase 13 655 +/- 36 806 +/- 40 539 +/- 37 736 +/- 36 0.019233825 0.004277603 0.193007684 0.08807373 MAP3K6 mitogen-activated protein kinase kinase kinase 6 292 +/- 47 544 +/- 98 200 +/- 27 272 +/- 34 0.051470939 0.13615307 0.066421173 0.015676532 MBP myelin basic protein 267 +/- 30 382 +/- 30 198 +/- 26 233 +/- 24 0.02215176 0.356889779 0.163209489 0.658828331 MCM3AP MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein 113 +/- 18 175 +/- 17 138 +/- 12 186 +/- 14 0.034417289 0.029901837 0.139021258 0.017434983 METTL5 methyltransferase like 5 537 +/- 35 732 +/- 38 613 +/- 18 740 +/- 26 0.003613606 0.004300567 0.06472819 0.139529211 MIER1 mesoderm induction early response 1 homolog (Xenopus laevis) 445 +/- 23 518 +/- 23 603 +/- 34 526 +/- 34 0.052128599 -0.139560385 0.197188748 0.070325535 MORC2 MORC family CW-type zinc finger 2 239 +/- 17 310 +/- 23 255 +/- 14 259 +/- 19 0.033010557 0.84791743 0.144557863 0.549322188 MPP5 membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) 1226 +/- 50 1391 +/- 42 1232 +/- 32 1396 +/- 65 0.031397285 0.06518596 0.073773107 0.439592422 MYLK myosin, light polypeptide kinase 2425 +/- 309 3340 +/- 241 1446 +/- 132 2443 +/- 131 0.043082979 0.000477748 0.11663479 0.069364667 MYSM1 myb-like, SWIRM and MPN domains 1 1621 +/- 104 1918 +/- 60 1905 +/- 135 2056 +/- 106 0.038833834 0.400278588 0.099193813 0.198163684 MYST1 MYST histone acetyltransferase 1 110 +/- 7 152 +/- 8 123 +/- 16 139 +/- 15 0.003906619 0.48476742 0.091778231 0.448618831 NAPB N-ethylmaleimide-sensitive factor attachment protein, beta 177 +/- 26 406 +/- 74 348 +/- 67 475 +/- 68 0.026112425 0.218482251 0.113400699 0.324157845 NCAM1 neural cell adhesion molecule 1 374 +/- 80 1218 +/- 237 262 +/- 43 746 +/- 266 0.014440982 0.142824454 0.093267691 0.563311732 NCKIPSD NCK interacting protein with SH3 domain 1420 +/- 128 2242 +/- 157 1411 +/- 94 2164 +/- 111 0.002447617 0.000744802 0.093724422 0.031940681 NFIA nuclear factor I/A 731 +/- 78 982 +/- 55 989 +/- 53 935 +/- 121 0.026750064 -0.697484863 0.103003403 0.177228163 NFX1 nuclear transcription factor, X-box binding 1 115 +/- 15 162 +/- 12 126 +/- 3 169 +/- 16 0.039838428 0.047698285 0.119763606 0.078436117 NISCH nischarin 262 +/- 23 343 +/- 27 377 +/- 20 423 +/- 44 0.04752027 0.383179785 0.171937937 0.043230983 NME1 Non-metastatic cells 1, protein (NM23A) expressed in 1207 +/- 74 1482 +/- 34 1218 +/- 92 1399 +/- 47 0.011642985 0.119898648 0.111929354 0.336369862 NPHP3 nephronophthisis 3 (adolescent) 113 +/- 9 157 +/- 10 147 +/- 18 189 +/- 8 0.008843705 0.070449903 0.06901726 0.136179994

S4- MSI correlated and non-correlated ARGs.xls Page 27 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG NPL N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase) 229 +/- 29 384 +/- 51 300 +/- 21 335 +/- 41 0.030694664 0.479417912 0.115841389 0.696042526 NSUN2 NOL1/NOP2/Sun domain family, member 2 521 +/- 29 653 +/- 50 633 +/- 51 677 +/- 39 0.053407057 0.513679036 0.298447921 0.863715442 NUDT5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 884 +/- 75 1105 +/- 67 672 +/- 11 954 +/- 63 0.053390579 0.010161315 0.094210476 0.12241466 NUP62 nucleoporin 62kDa 885 +/- 11 1158 +/- 43 1053 +/- 49 1241 +/- 35 0.000988177 0.012690922 0.069716696 0.04182895 NUPL1 nucleoporin like 1 80 +/- 12 133 +/- 19 138 +/- 7 175 +/- 24 0.043676096 0.212114005 0.235786247 0.828518206 OGFRL1 opioid growth factor receptor-like 1 121 +/- 12 168 +/- 17 211 +/- 16 189 +/- 23 0.054419173 -0.447763356 0.056448241 0.476359997 PAIP2 poly(A) binding protein interacting protein 2 6014 +/- 283 6884 +/- 216 5284 +/- 145 5886 +/- 240 0.036388176 0.070780543 0.095169664 0.284790024 PAQR8 progestin and adipoQ receptor family member VIII 3877 +/- 236 4674 +/- 31 4024 +/- 144 4047 +/- 240 0.019111631 0.93948212 0.100311337 0.396077656 PCF11 PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae) 929 +/- 93 1221 +/- 73 1180 +/- 136 1270 +/- 151 0.03469835 0.669485315 0.093413738 0.768232263 PDGFA platelet-derived growth factor alpha polypeptide 71 +/- 16 134 +/- 20 87 +/- 13 170 +/- 42 0.035864061 0.119291863 0.133664284 0.096099286 PDLIM4 PDZ and LIM domain 4 397 +/- 29 502 +/- 35 398 +/- 27 477 +/- 30 0.045611451 0.083153202 0.059789114 0.298752675 PGAM1 phosphoglycerate mutase 1 (brain) 11785 +/- 417 13568 +/- 474 10224 +/- 320 12402 +/- 471 0.018304155 0.006017659 0.146932074 0.039091516 PHC3 Polyhomeotic like 3 (Drosophila) 83 +/- 5 113 +/- 9 127 +/- 13 127 +/- 17 0.025432255 -0.986452201 0.074767882 0.431370145 PIGZ phosphatidylinositol glycan, class Z 644 +/- 33 916 +/- 90 686 +/- 41 941 +/- 35 0.027433302 0.001051162 0.094046979 0.015991583 PKN1 protein kinase N1 1025 +/- 87 1327 +/- 101 952 +/- 42 1034 +/- 60 0.047977137 0.299131293 0.111437103 0.507674326 PKP4 plakophilin 4 192 +/- 40 356 +/- 46 228 +/- 41 321 +/- 34 0.023207386 0.11621468 0.149577903 0.066711467 PLCH2 phospholipase C, eta 2 370 +/- 51 502 +/- 14 513 +/- 63 801 +/- 59 0.049362678 0.008956677 0.067947152 0.087632183 PLEKHA6 pleckstrin homology domain containing, family A member 6 566 +/- 46 738 +/- 53 585 +/- 29 732 +/- 40 0.034289835 0.018921166 0.162664897 0.080752046 PLK1 polo-like kinase 1 (Drosophila) 21403 +/- 877 23680 +/- 475 19863 +/- 1093 22695 +/- 710 0.053081378 0.060485904 0.350126365 0.318981193 PMS2 PMS2 postmeiotic segregation increased 2 (S. cerevisiae) 429 +/- 49 1254 +/- 258 530 +/- 58 1843 +/- 341 0.023149503 0.017204806 0.200908195 0.119468895 POLD2 Polymerase (DNA directed), delta 2, regulatory subunit 50kDa 88 +/- 13 142 +/- 12 136 +/- 20 197 +/- 12 0.01183106 0.028238118 0.079666315 0.256120179 PPAP2B phosphatidic acid phosphatase type 2B 2720 +/- 167 3718 +/- 213 2634 +/- 127 2728 +/- 239 0.004571231 0.741309938 0.063156923 0.390909034 PRKCH protein kinase C, eta 181 +/- 18 255 +/- 18 129 +/- 12 131 +/- 19 0.016863206 0.94345249 0.130287445 0.854606822 PRR13 proline rich 13 3251 +/- 117 3856 +/- 164 2723 +/- 24 3074 +/- 150 0.014722864 0.079233542 0.086049672 0.184989891 PRRX2 paired related homeobox 2 228 +/- 13 276 +/- 7 263 +/- 27 273 +/- 24 0.012613526 0.791143164 0.119347312 0.78923988 PSCD4 pleckstrin homology, Sec7 and coiled-coil domains 4 213 +/- 21 291 +/- 13 199 +/- 18 202 +/- 16 0.01228804 0.924077015 0.094947689 0.484449893 PSMB7 Proteasome (prosome, macropain) subunit, beta type, 7 371 +/- 27 515 +/- 39 467 +/- 43 650 +/- 85 0.014376321 0.103112853 0.068983094 0.453466573 PSMC5 proteasome (prosome, macropain) 26S subunit, ATPase, 5 4810 +/- 47 5639 +/- 234 4899 +/- 178 5254 +/- 108 0.01560899 0.125387519 0.183499417 0.306443488 PSMD4 proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 2476 +/- 113 2897 +/- 73 2016 +/- 75 2132 +/- 95 0.012706839 0.364097738 0.09970474 0.508662756 PTK2 PTK2 protein tyrosine kinase 2 352 +/- 24 487 +/- 29 419 +/- 31 528 +/- 44 0.005345001 0.081021869 0.05936811 0.141248224 PTP4A3 protein tyrosine phosphatase type IVA, member 3 107 +/- 7 143 +/- 10 103 +/- 8 139 +/- 10 0.013215792 0.023195861 0.197196097 0.071717067 PTPN21 Protein tyrosine phosphatase, non-receptor type 21 156 +/- 37 305 +/- 48 153 +/- 15 276 +/- 31 0.036045999 0.013348896 0.104382719 0.018237476 RABL4 RAB, member of RAS oncogene family-like 4 740 +/- 95 1128 +/- 110 549 +/- 56 835 +/- 106 0.023895769 0.054057633 0.076826758 0.321291487 RANBP5 RAN binding protein 5 936 +/- 51 1233 +/- 94 1110 +/- 54 1359 +/- 44 0.025319009 0.005955687 0.189239579 0.186509537 RBM17 RNA binding motif protein 17 269 +/- 33 382 +/- 35 305 +/- 29 360 +/- 17 0.041351699 0.14662783 0.105831814 0.496468875 RENBP renin binding protein 106 +/- 14 148 +/- 12 134 +/- 15 152 +/- 8 0.053117985 0.333910173 0.156043251 0.278461581 RGS11 regulator of G-protein signalling 11 262 +/- 54 525 +/- 62 397 +/- 50 606 +/- 54 0.009410819 0.019498241 0.076190609 0.026970839 RINT1 RAD50 interactor 1 379 +/- 15 428 +/- 6 425 +/- 14 401 +/- 17 0.017524088 -0.303252578 0.091312897 0.075998411 RIPK2 receptor-interacting serine-threonine kinase 2 475 +/- 23 551 +/- 22 420 +/- 19 435 +/- 19 0.040022958 0.590227599 0.259237704 0.969415904 RNASEH1 ribonuclease H1 129 +/- 15 178 +/- 14 148 +/- 7 241 +/- 18 0.041606548 0.00463307 0.229431039 0.032545623 RNASEH2A ribonuclease H2, large subunit 227 +/- 15 280 +/- 16 209 +/- 14 224 +/- 16 0.037496755 0.500722271 0.127715477 0.768173717 RNPS1 RNA binding protein S1, serine-rich domain 2748 +/- 78 3124 +/- 133 3034 +/- 149 3248 +/- 132 0.040859818 0.310943278 0.216154106 0.447975573 RP2 retinitis pigmentosa 2 (X-linked recessive) 105 +/- 12 148 +/- 15 124 +/- 13 142 +/- 17 0.047085506 0.433553079 0.075616688 0.913039696 RPL24 ribosomal protein L24 6992 +/- 211 7638 +/- 185 6738 +/- 313 6697 +/- 239 0.044417687 -0.919545644 0.133596205 0.575328242

S4- MSI correlated and non-correlated ARGs.xls Page 28 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG RPL36AL ribosomal protein L36a-like 2981 +/- 70 3410 +/- 137 2856 +/- 113 3117 +/- 174 0.025652764 0.246811545 0.126170814 0.073202304 RYBP RING1 and YY1 binding protein 922 +/- 102 1454 +/- 142 927 +/- 96 1306 +/- 164 0.013634466 0.088025095 0.068890175 0.312410297 S100A6 S100 calcium binding protein A6 (calcyclin) 923 +/- 64 1156 +/- 72 682 +/- 81 1085 +/- 58 0.035962432 0.003149417 0.220246563 0.1084609 SDS serine dehydratase 467 +/- 22 597 +/- 50 542 +/- 35 590 +/- 52 0.048647881 0.469206805 0.31742044 0.68039198 SELPLG selectin P ligand 660 +/- 60 886 +/- 79 469 +/- 46 573 +/- 50 0.047568029 0.159000541 0.055121434 0.325228323 SERPINB6 serpin peptidase inhibitor, clade B (ovalbumin), member 6 139 +/- 16 197 +/- 19 232 +/- 20 249 +/- 10 0.040554888 0.469388785 0.204045641 0.772834173 SERPINB8 serpin peptidase inhibitor, clade B (ovalbumin), member 8 103 +/- 14 142 +/- 10 101 +/- 7 125 +/- 8 0.0451369 0.047732079 0.210402796 0.567889163 SERPINF1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 508 +/- 96 868 +/- 124 280 +/- 49 579 +/- 121 0.046244604 0.067889475 0.204822794 0.17769943 SLC12A9 solute carrier family 12 (potassium/chloride transporters), member 9 1093 +/- 101 1413 +/- 80 1023 +/- 47 1257 +/- 43 0.033133089 0.005050841 0.144496165 0.152042307 SLC22A18 solute carrier family 22 (organic cation transporter), member 18 269 +/- 23 400 +/- 39 191 +/- 11 317 +/- 21 0.019838905 0.001619511 0.11369647 0.044955166 SLC26A2 solute carrier family 26 (sulfate transporter), member 2 87 +/- 10 217 +/- 49 135 +/- 50 223 +/- 61 0.045721894 0.294341205 0.128849196 0.252088842 SLC37A4 solute carrier family 37 (glycerol-6-phosphate transporter), member 4 195 +/- 20 266 +/- 23 170 +/- 18 236 +/- 25 0.04461133 0.068064874 0.174647238 0.276966572 SLC39A4 solute carrier family 39 (zinc transporter), member 4 534 +/- 35 698 +/- 42 516 +/- 28 599 +/- 44 0.014272734 0.152656506 0.216545695 0.061295951 SNAPC5 small nuclear RNA activating complex, polypeptide 5, 19kDa 221 +/- 25 289 +/- 15 232 +/- 9 285 +/- 45 0.045724345 0.302886724 0.260286025 0.490432514 SOHLH2 spermatogenesis and oogenesis specific basic helix-loop-helix 2 101 +/- 10 163 +/- 15 88 +/- 6 159 +/- 11 0.007052751 0.001346771 0.113962623 0.033681699 SRP19 signal recognition particle 19kDa 2698 +/- 166 3239 +/- 67 2572 +/- 101 3210 +/- 30 0.021094707 0.001032739 0.112768564 0.012163432 SS18L2 synovial sarcoma translocation gene on chromosome 18-like 2 2273 +/- 100 2573 +/- 55 2299 +/- 38 2484 +/- 84 0.030670736 0.093922649 0.066332766 0.018151952 SSR3 signal sequence receptor, gamma (translocon-associated protein gamma) 1416 +/- 40 1776 +/- 112 1570 +/- 46 1920 +/- 158 0.022161034 0.090364437 0.152255232 0.636182123 SSU72 SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae) 2015 +/- 93 2625 +/- 115 1716 +/- 93 2050 +/- 54 0.002266345 0.015168408 0.111942881 0.067295625 STAT5B signal transducer and activator of transcription 5B 521 +/- 41 647 +/- 33 390 +/- 30 402 +/- 19 0.037838558 0.730509064 0.095960107 0.569242292 STOM stomatin 2053 +/- 253 2990 +/- 261 1953 +/- 163 2755 +/- 171 0.027515679 0.008200839 0.063237653 0.157507683 TALDO1 transaldolase 1 955 +/- 59 1652 +/- 264 1013 +/- 64 1727 +/- 292 0.045456477 0.069640764 0.071866139 0.051387335 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) 270 +/- 14 334 +/- 23 295 +/- 13 305 +/- 17 0.046261676 0.667672576 0.413727731 0.609393915 TATDN3 TatD DNase domain containing 3 116 +/- 9 169 +/- 11 138 +/- 16 192 +/- 10 0.0041436 0.02164698 0.07241459 0.055340933 TBC1D2B TBC1 domain family, member 2B 261 +/- 20 351 +/- 28 327 +/- 28 322 +/- 34 0.028369212 -0.92466454 0.089305832 0.475598917 TCEAL4 transcription elongation factor A (SII)-like 4 8509 +/- 229 10384 +/- 384 8133 +/- 200 10329 +/- 553 0.00289964 0.013325761 0.097909518 0.138275737 TMED4 transmembrane emp24 protein transport domain containing 4 1134 +/- 55 1431 +/- 104 1087 +/- 50 1361 +/- 59 0.036499694 0.007200925 0.077299985 0.024735739 TMEM106C transmembrane protein 106C 1970 +/- 98 2345 +/- 128 1940 +/- 209 2804 +/- 219 0.043413259 0.019261834 0.312849989 0.22733026 TMEM11 transmembrane protein 11 845 +/- 33 981 +/- 25 769 +/- 31 945 +/- 37 0.00892987 0.006195045 0.139897829 0.106812496 TMEM51 transmembrane protein 51 459 +/- 34 555 +/- 15 354 +/- 21 396 +/- 28 0.038460501 0.277147916 0.135615529 0.595645431 TMEM62 transmembrane protein 62 272 +/- 20 338 +/- 21 254 +/- 9 273 +/- 20 0.050413093 0.433539011 0.06655727 0.798808819 TMEM87B Transmembrane protein 87B 96 +/- 30 190 +/- 26 87 +/- 22 198 +/- 10 0.039793595 0.002630566 0.180729609 0.020908784 TPD52 tumor protein D52 200 +/- 13 258 +/- 21 346 +/- 17 380 +/- 12 0.047386657 0.132678126 0.15779761 0.243533556 TRIO Triple functional domain (PTPRF interacting) 232 +/- 36 388 +/- 49 416 +/- 22 423 +/- 36 0.030345502 0.867578535 0.151460038 0.623949123 TRIP6 thyroid hormone receptor interactor 6 448 +/- 39 566 +/- 21 435 +/- 17 459 +/- 14 0.030822702 0.305348649 0.129142373 0.855060689 TRMT12 tRNA methyltranferase 12 homolog (S. cerevisiae) 506 +/- 22 617 +/- 34 498 +/- 40 575 +/- 20 0.023410365 0.128590246 0.350543775 0.455650939 TRUB2 TruB pseudouridine (psi) synthase homolog 2 (E. coli) 855 +/- 93 1100 +/- 56 795 +/- 42 1011 +/- 37 0.052142304 0.003952869 0.128420178 0.100419906 TSC22D2 TSC22 domain family, member 2 2203 +/- 83 2607 +/- 109 2346 +/- 100 2554 +/- 91 0.015867316 0.157062537 0.065162361 0.594418259 TSEN34 tRNA splicing endonuclease 34 homolog (S. cerevisiae) 991 +/- 52 1198 +/- 77 784 +/- 49 985 +/- 39 0.052950505 0.010934018 0.353018347 0.022477243 TTC27 tetratricopeptide repeat domain 27 442 +/- 31 538 +/- 25 493 +/- 26 422 +/- 31 0.039954075 -0.12036136 0.161221328 0.218502308 TTF1 transcription termination factor, RNA polymerase I 700 +/- 25 781 +/- 23 793 +/- 51 789 +/- 50 0.039866804 -0.961344327 0.075517401 0.738303819 UNC84A unc-84 homolog A (C. elegans) 302 +/- 15 412 +/- 31 411 +/- 55 383 +/- 30 0.014892189 -0.669886746 0.115425415 0.865350755 USP31 ubiquitin specific peptidase 31 360 +/- 19 436 +/- 27 499 +/- 36 687 +/- 45 0.045849929 0.011486388 0.133505683 0.121915743 USP53 ubiquitin specific peptidase 53 346 +/- 23 476 +/- 40 443 +/- 44 496 +/- 19 0.022037695 0.304396782 0.149763107 0.639937165

S4- MSI correlated and non-correlated ARGs.xls Page 29 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG VASH1 Vasohibin 1 535 +/- 16 744 +/- 63 1011 +/- 90 999 +/- 53 0.019830604 -0.908354959 0.071398018 0.915214345 WDR71 WD repeat domain 71 1094 +/- 63 1355 +/- 56 978 +/- 43 1086 +/- 116 0.011098704 0.422751508 0.096365296 0.765643542 WHSC1 Wolf-Hirschhorn syndrome candidate 1 291 +/- 18 359 +/- 21 296 +/- 30 337 +/- 23 0.033598182 0.306910115 0.057417704 0.216523417 XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku a 1545 +/- 90 1864 +/- 59 1424 +/- 33 1774 +/- 97 0.016491456 0.01936134 0.248647388 0.118649987 YME1L1 YME1-like 1 (S. cerevisiae) 3560 +/- 193 4334 +/- 244 3653 +/- 205 4168 +/- 128 0.033153059 0.065747004 0.355158898 0.303655244 ZC3H5 zinc finger CCCH-type containing 5 511 +/- 50 651 +/- 39 464 +/- 35 503 +/- 41 0.053680213 0.490227473 0.186474076 0.558839087 ZFP90 zinc finger protein 90 homolog (mouse) 3528 +/- 279 4260 +/- 161 3870 +/- 345 4323 +/- 157 0.052575066 0.270958779 0.091568156 0.4421446 ZNF131 Zinc finger protein 131 (clone pHZ-10) 124 +/- 18 238 +/- 29 160 +/- 22 267 +/- 25 0.010052177 0.011395349 0.126156615 0.066808291 ZNF25 zinc finger protein 25 1239 +/- 41 1519 +/- 51 1305 +/- 50 1653 +/- 73 0.001854661 0.005249539 0.085885628 0.065991508 ZNF278 zinc finger protein 278 239 +/- 11 277 +/- 12 317 +/- 19 300 +/- 16 0.042714892 -0.486719149 0.060185824 0.746507111 ZNF358 zinc finger protein 358 556 +/- 13 684 +/- 43 872 +/- 81 877 +/- 12 0.02865496 0.953890637 0.086471072 0.979644641 ZNF45 zinc finger protein 45 279 +/- 18 336 +/- 17 332 +/- 22 400 +/- 18 0.049501338 0.045353643 0.241044901 0.225942957 ZNF564 zinc finger protein 564 324 +/- 32 430 +/- 24 307 +/- 19 390 +/- 12 0.02532311 0.006175825 0.087713265 0.046735044 ZRANB1 zinc finger, RAN-binding domain containing 1 849 +/- 44 1040 +/- 45 887 +/- 57 1073 +/- 52 0.012514974 0.039302018 0.25207767 0.26501462 Downregulated with Age in CA, not MSI Correlated ACADL acyl-Coenzyme A dehydrogenase, long chain 234 +/- 12 184 +/- 12 226 +/- 9 179 +/- 19 -0.013598903 -0.065476551 0.095939092 0.084506896 ACAT1 acetyl-Coenzyme A acetyltransferase 1 (acetoacetyl Coenzyme A thiolase) 4099 +/- 188 3515 +/- 106 3250 +/- 137 3238 +/- 137 -0.027045728 -0.954273333 0.112810616 0.970582072 AFTIPHILIN aftiphilin protein 689 +/- 9 552 +/- 54 739 +/- 50 699 +/- 28 -0.050685083 -0.50296709 0.135389216 0.618589192 AHSA1 AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast) 3672 +/- 96 3351 +/- 98 3165 +/- 122 3121 +/- 103 -0.041810364 -0.788980672 0.074586308 0.708170741 AKAP8L A kinase (PRKA) anchor protein 8-like 115 +/- 15 71 +/- 9 120 +/- 16 99 +/- 3 -0.038473309 -0.237324381 0.094010653 0.464845005 ANAPC1 anaphase promoting complex subunit 1 942 +/- 48 810 +/- 32 776 +/- 30 736 +/- 25 -0.048251996 -0.328607776 0.096262188 0.410849046 APLP2 Amyloid beta (A4) precursor-like protein 2 397 +/- 24 319 +/- 21 448 +/- 28 403 +/- 22 -0.037329387 -0.240439165 0.077951047 0.515907206 ARHGAP26 Rho GTPase activating protein 26 1897 +/- 171 1395 +/- 80 1867 +/- 154 1491 +/- 130 -0.031968056 -0.09582784 0.095924755 0.130662311 ARIH2 ariadne homolog 2 (Drosophila) 1295 +/- 30 1073 +/- 49 1029 +/- 41 1044 +/- 54 -0.004512849 0.829441242 0.114765113 0.77566376 ASAHL N-acylsphingosine amidohydrolase (acid ceramidase)-like 157 +/- 9 116 +/- 12 192 +/- 21 128 +/- 16 -0.026696429 -0.03809744 0.059545479 0.032905844 ASB6 ankyrin repeat and SOCS box-containing 6 176 +/- 8 150 +/- 8 238 +/- 19 208 +/- 4 -0.052556972 -0.185876983 0.185406644 0.328432717 ATP5D ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit 1719 +/- 90 1425 +/- 89 2012 +/- 103 1836 +/- 84 -0.0426828 -0.215554546 0.19475628 0.608712053 ATP8B2 ATPase, Class I, type 8B, member 2 1260 +/- 36 1102 +/- 43 1508 +/- 74 1279 +/- 39 -0.018717994 -0.027391031 0.071600827 0.129279316 BICD2 bicaudal D homolog 2 (Drosophila) 1123 +/- 23 818 +/- 85 1051 +/- 110 902 +/- 129 -0.014435203 -0.404725053 0.353502736 0.79558013 BOP1 block of proliferation 1 292 +/- 18 210 +/- 26 357 +/- 15 258 +/- 9 -0.026404469 -0.000563916 0.093683447 0.002243383 BRD2 bromodomain containing 2 683 +/- 52 469 +/- 53 945 +/- 41 572 +/- 72 -0.016524852 -0.003473977 0.280047378 0.097054419 BRWD2 bromodomain and WD repeat domain containing 2 564 +/- 69 382 +/- 3 593 +/- 41 404 +/- 22 -0.046177058 -0.004233115 0.137620747 0.03576959 BUB3 BUB3 budding uninhibited by benzimidazoles 3 homolog (yeast) 4140 +/- 127 3656 +/- 142 3469 +/- 169 3052 +/- 117 -0.029420059 -0.074617994 0.249553418 0.146021249 CACNA1A calcium channel, voltage-dependent, P/Q type, alpha 1A subunit 961 +/- 89 664 +/- 44 1617 +/- 110 993 +/- 119 -0.018644654 -0.00419325 0.07476412 0.121622277 CBARA1 calcium binding atopy-related autoantigen 1 1698 +/- 52 1549 +/- 37 1445 +/- 72 1507 +/- 58 -0.044069618 0.517736925 0.140672141 0.701144959 CCDC8 coiled-coil domain containing 8 394 +/- 24 308 +/- 20 374 +/- 23 310 +/- 19 -0.020152331 -0.059179115 0.135753437 0.193514213 CCT7 chaperonin containing TCP1, subunit 7 (eta) 926 +/- 48 785 +/- 35 940 +/- 51 822 +/- 54 -0.039816803 -0.146650758 0.091686639 0.838260292 CD5 CD5 molecule 143 +/- 6 90 +/- 18 125 +/- 19 122 +/- 17 -0.034030857 -0.906423285 0.232602789 0.680417185 CEP63 centrosomal protein 63kDa 153 +/- 12 107 +/- 16 136 +/- 25 82 +/- 13 -0.045694317 -0.097117154 0.437954492 0.368089469 CKAP1 cytoskeleton associated protein 1 1486 +/- 142 1105 +/- 70 1406 +/- 119 962 +/- 130 -0.04564535 -0.033776237 0.283899216 0.20179708 CLEC14A C-type lectin domain family 14, member A 139 +/- 9 108 +/- 10 129 +/- 11 111 +/- 13 -0.040997297 -0.334514157 0.225561185 0.832977876 CLK4 CDC-like kinase 4 2680 +/- 52 2262 +/- 87 2336 +/- 119 1847 +/- 72 -0.003236721 -0.007870411 0.089082266 0.093570671 CNTNAP2 contactin associated protein-like 2 4333 +/- 298 3178 +/- 404 3580 +/- 316 3606 +/- 414 -0.046274165 0.961216982 0.064276853 0.663013879 CORO7 coronin 7 1060 +/- 57 830 +/- 78 1137 +/- 43 970 +/- 97 -0.040219064 -0.170909038 0.127917319 0.532821353

S4- MSI correlated and non-correlated ARGs.xls Page 30 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG CREBBP CREB binding protein (Rubinstein-Taybi syndrome) 1537 +/- 76 1340 +/- 29 1670 +/- 25 1368 +/- 90 -0.047722227 -0.026138044 0.218216685 0.238085085 DCTN4 dynactin 4 (p62) 2167 +/- 114 1786 +/- 115 2057 +/- 72 1769 +/- 87 -0.03959171 -0.033853445 0.439314547 0.327635314 DFFA DNA fragmentation factor, 45kDa, alpha polypeptide 632 +/- 34 537 +/- 27 694 +/- 27 593 +/- 28 -0.054305353 -0.031207415 0.19627277 0.297678699 DKFZP779L1PRO0845 1349 +/- 59 1055 +/- 62 1202 +/- 31 955 +/- 46 -0.006254246 -0.002851053 0.117590109 0.084762499 DNAJB6 DnaJ (Hsp40) homolog, subfamily B, member 6 3673 +/- 317 2730 +/- 233 3649 +/- 170 3344 +/- 450 -0.039541985 -0.553793572 0.055344944 0.406183815 DNPEP aspartyl aminopeptidase 711 +/- 27 636 +/- 18 663 +/- 42 616 +/- 40 -0.04453319 -0.436049287 0.09261531 0.349729268 EBF2 early B-cell factor 2 119 +/- 5 103 +/- 3 119 +/- 8 91 +/- 19 -0.028084204 -0.241927208 0.082061252 0.405829609 EGLN1 egl nine homolog 1 (C. elegans) 599 +/- 67 426 +/- 24 799 +/- 81 561 +/- 31 -0.050058466 -0.031492154 0.074236615 0.052091752 EIF3S9 eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa 3204 +/- 180 2481 +/- 170 2961 +/- 100 2491 +/- 175 -0.01552974 -0.055558645 0.121320466 0.49331045 ETF1 eukaryotic translation termination factor 1 243 +/- 4 206 +/- 11 313 +/- 29 253 +/- 16 -0.015712267 -0.113143778 0.073688839 0.171391325 FBXL14 F-box and leucine-rich repeat protein 14 1117 +/- 114 749 +/- 52 1272 +/- 40 979 +/- 38 -0.022120684 -0.000484356 0.075790002 0.03101781 FBXL5 F-box and leucine-rich repeat protein 5 281 +/- 17 233 +/- 9 307 +/- 13 264 +/- 18 -0.036267776 -0.084253276 0.102507593 0.237619367 FLJ42875 FLJ42875 protein 320 +/- 16 256 +/- 17 326 +/- 29 256 +/- 19 -0.019359676 -0.079188515 0.316538742 0.154758509 FRMD4A FERM domain containing 4A 926 +/- 37 796 +/- 36 829 +/- 20 663 +/- 39 -0.03071271 -0.008664546 0.131708592 0.03971463 GALNTL2 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 448 +/- 45 296 +/- 38 272 +/- 18 235 +/- 32 -0.026776319 -0.34309929 0.089751425 0.366674468 GGTL3 gamma-glutamyltransferase-like 3 2529 +/- 133 2114 +/- 121 2837 +/- 156 2630 +/- 103 -0.044208216 -0.298117483 0.1027986 0.493083164 GK glycerol kinase 118 +/- 7 90 +/- 9 112 +/- 5 102 +/- 8 -0.034464658 -0.312875449 0.10037188 0.146850481 GORASP2 golgi reassembly stacking protein 2, 55kDa 1888 +/- 69 1700 +/- 21 1893 +/- 58 1861 +/- 71 -0.040739466 -0.737723285 0.160559526 0.635123478 GRSF1 G-rich RNA sequence binding factor 1 2254 +/- 87 1851 +/- 104 2017 +/- 89 2006 +/- 155 -0.014164872 -0.955008368 0.178716788 0.278694007 GTF2IRD2 GTF2I repeat domain containing 2 310 +/- 18 224 +/- 27 365 +/- 33 210 +/- 55 -0.028055011 -0.047209612 0.482695937 0.339189571 HNRPA3P1 heterogeneous nuclear ribonucleoprotein A3 pseudogene 1 1370 +/- 59 1182 +/- 49 1527 +/- 55 1349 +/- 65 -0.033981032 -0.06724799 0.159137479 0.200331911 HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 166 +/- 21 103 +/- 13 182 +/- 25 126 +/- 12 -0.032309566 -0.078401472 0.251783708 0.11535464 HYLS1 hydrolethalus syndrome 1 748 +/- 26 632 +/- 33 635 +/- 12 572 +/- 47 -0.020014447 -0.253758296 0.058006283 0.963550522 ISG20L1 interferon stimulated exonuclease gene 20kDa-like 1 320 +/- 14 256 +/- 21 292 +/- 17 271 +/- 4 -0.034363224 -0.270544755 0.331455782 0.529138598 ITCH itchy homolog E3 ubiquitin protein ligase (mouse) 198 +/- 29 78 +/- 10 178 +/- 21 75 +/- 12 -0.007101125 -0.002594673 0.06165396 0.02277359 KCNH2 potassium voltage-gated channel, subfamily H (eag-related), member 2 579 +/- 45 458 +/- 18 529 +/- 26 467 +/- 29 -0.043132112 -0.147815318 0.100719089 0.50597957 KCNIP1 Kv channel interacting protein 1 233 +/- 13 189 +/- 12 243 +/- 17 208 +/- 14 -0.033419928 -0.148766965 0.240521615 0.027191385 KCNK17 potassium channel, subfamily K, member 17 175 +/- 17 111 +/- 10 253 +/- 20 177 +/- 20 -0.011001664 -0.023816161 0.072727066 0.084128799 KCNQ2 potassium voltage-gated channel, KQT-like subfamily, member 2 279 +/- 12 234 +/- 12 261 +/- 17 237 +/- 14 -0.025523326 -0.286495366 0.170292018 0.331447035 KIF21A kinesin family member 21A 3821 +/- 217 2757 +/- 229 4422 +/- 302 3119 +/- 95 -0.007042682 -0.006370346 0.063541337 0.033469949 KLF13 Kruppel-like factor 13 1790 +/- 60 1158 +/- 151 2000 +/- 133 1313 +/- 196 -0.006850529 -0.022009748 0.148842536 0.386259152 LETM2 leucine zipper-EF-hand containing transmembrane protein 2 125 +/- 7 98 +/- 8 102 +/- 12 112 +/- 8 -0.025883089 0.50170882 0.098267951 0.334409248 LRP11 low density lipoprotein receptor-related protein 11 4775 +/- 167 4237 +/- 116 4754 +/- 143 4135 +/- 226 -0.026691143 -0.054358382 0.213604269 0.477677495 LSM8 LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae) 638 +/- 35 457 +/- 26 600 +/- 28 455 +/- 21 -0.002364057 -0.00265354 0.113069846 0.050929646 LTBP1 latent transforming growth factor beta binding protein 1 361 +/- 17 236 +/- 20 447 +/- 38 218 +/- 28 -0.00071457 -0.000958711 0.098246847 0.0743978 MAML2 Mastermind-like 2 (Drosophila) 200 +/- 11 150 +/- 14 192 +/- 13 180 +/- 9 -0.018401983 -0.467974892 0.081825474 0.371377257 MAP1LC3A microtubule-associated protein 1 light chain 3 alpha 3967 +/- 274 2363 +/- 245 4800 +/- 253 2961 +/- 404 -0.00144192 -0.00636558 0.059834641 0.163614208 MAP4K2 mitogen-activated protein kinase kinase kinase kinase 2 313 +/- 12 258 +/- 18 306 +/- 26 255 +/- 7 -0.032048187 -0.101176641 0.061029039 0.336908863 MARK2 MAP/microtubule affinity-regulating kinase 2 823 +/- 33 702 +/- 30 941 +/- 40 765 +/- 25 -0.023035541 -0.005706683 0.135183632 0.132393049 MASP1 mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor) 248 +/- 14 181 +/- 18 233 +/- 11 210 +/- 10 -0.013608526 -0.157176111 0.076589254 0.472558657 MAX MYC associated factor X 1031 +/- 87 779 +/- 39 1103 +/- 54 911 +/- 39 -0.034213781 -0.019564295 0.067448525 0.207610623 MCOLN1 mucolipin 1 869 +/- 34 752 +/- 20 733 +/- 41 707 +/- 21 -0.019150725 -0.582761891 0.117746827 0.69446208 MDH1B malate dehydrogenase 1B, NAD (soluble) 438 +/- 14 356 +/- 17 295 +/- 17 277 +/- 4 -0.004634236 -0.331350201 0.064732039 0.410328189 MED19 mediator of RNA polymerase II transcription, subunit 19 homolog (S. cerevisiae) 1426 +/- 32 1306 +/- 36 1307 +/- 44 1177 +/- 45 -0.032648163 -0.069494943 0.287068914 0.296589671

S4- MSI correlated and non-correlated ARGs.xls Page 31 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG MEIS3 Meis1, myeloid ecotropic viral integration site 1 homolog 3 (mouse) 654 +/- 42 481 +/- 45 714 +/- 138 476 +/- 75 -0.019556222 -0.170504977 0.211309688 0.481349059 METT11D1 methyltransferase 11 domain containing 1 227 +/- 25 163 +/- 12 203 +/- 17 195 +/- 18 -0.04992278 -0.753511612 0.064328415 0.489343761 MLL5 myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila) 1973 +/- 306 931 +/- 182 2063 +/- 365 760 +/- 108 -0.018738458 -0.014618871 0.060188689 0.038757976 MNS1 meiosis-specific nuclear structural 1 261 +/- 32 177 +/- 16 182 +/- 17 182 +/- 19 -0.048125733 0.990467511 0.077835709 0.921570071 MOBKL2A MOB1, Mps One Binder kinase activator-like 2A (yeast) 166 +/- 17 108 +/- 15 199 +/- 13 144 +/- 22 -0.030365997 -0.067489025 0.177654419 0.013714181 MOBKL2B MOB1, Mps One Binder kinase activator-like 2B (yeast) 138 +/- 10 100 +/- 11 127 +/- 7 86 +/- 4 -0.028521632 -0.001403645 0.062958159 0.055919928 MPP6 membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) 324 +/- 28 239 +/- 22 340 +/- 27 328 +/- 27 -0.038754208 -0.752352879 0.060388315 0.912007047 MTHFD2L methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like 641 +/- 53 440 +/- 16 569 +/- 21 415 +/- 23 -0.01097374 -0.000813626 0.082986138 0.023699547 NCOA5 nuclear receptor coactivator 5 279 +/- 3 222 +/- 19 348 +/- 18 305 +/- 13 -0.02781036 -0.088612818 0.076350182 0.225401731 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive 3892 +/- 75 3653 +/- 71 3843 +/- 56 3902 +/- 34 -0.043531938 0.390589883 0.0610563 0.353576388 OGT O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucos 1094 +/- 60 899 +/- 25 1800 +/- 83 1694 +/- 67 -0.022120839 -0.348760409 0.17190686 0.652687815 ORC4L origin recognition complex, subunit 4-like (yeast) 941 +/- 34 812 +/- 27 846 +/- 20 797 +/- 54 -0.014334478 -0.430875402 0.073147755 0.582952723 OSBPL6 oxysterol binding protein-like 6 2412 +/- 155 1905 +/- 45 2468 +/- 135 1963 +/- 75 -0.02064243 -0.012145114 0.093437471 0.227481628 PABPC5 poly(A) binding protein, cytoplasmic 5 153 +/- 5 103 +/- 10 160 +/- 15 110 +/- 8 -0.003193097 -0.019984793 0.089753987 0.125170727 PALMD palmdelphin 203 +/- 17 151 +/- 13 248 +/- 26 204 +/- 20 -0.039876333 -0.211303292 0.202666039 0.239841698 PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) 468 +/- 21 394 +/- 23 454 +/- 37 444 +/- 37 -0.042901277 -0.846960946 0.099304223 0.606204694 PHF20 PHD finger protein 20 592 +/- 14 520 +/- 25 526 +/- 18 503 +/- 18 -0.036912135 -0.38866108 0.18622594 0.853931386 PIGG phosphatidylinositol glycan, class G 748 +/- 78 456 +/- 62 758 +/- 104 521 +/- 43 -0.015859394 -0.075387155 0.095884911 0.25581642 PIP5K3 phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III 1685 +/- 66 1442 +/- 57 1387 +/- 56 1232 +/- 71 -0.020365624 -0.126116352 0.179359707 0.222707977 PLEKHA5 Pleckstrin homology domain containing, family A member 5 566 +/- 51 398 +/- 55 952 +/- 56 599 +/- 55 -0.048982668 -0.001487041 0.094460525 0.045348518 PLK2 polo-like kinase 2 (Drosophila) 4576 +/- 519 3141 +/- 223 3989 +/- 267 3502 +/- 175 -0.039743426 -0.163947479 0.075994905 0.716639141 PLXNA2 Plexin A2 261 +/- 24 167 +/- 18 177 +/- 13 194 +/- 14 -0.011534762 0.392874415 0.094225635 0.657815901 PNLDC1 poly(A)-specific ribonuclease (PARN)-like domain containing 1 203 +/- 62 42 +/- 1 212 +/- 57 60 +/- 6 -0.047861142 -0.044611804 0.195803754 0.13121375 POLR2J polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa 2255 +/- 115 1934 +/- 54 2042 +/- 31 1942 +/- 47 -0.039376867 -0.118458131 0.065244338 0.176140865 PPP2R5C protein phosphatase 2, regulatory subunit B (B56), gamma isoform 1380 +/- 94 967 +/- 75 1220 +/- 33 860 +/- 51 -0.006726147 -0.000552639 0.108054221 0.023206134 PROCA1 proline-rich cyclin A1-interacting protein 188 +/- 17 132 +/- 6 195 +/- 11 162 +/- 7 -0.01904541 -0.03118106 0.104932479 0.258450261 PSMA6 proteasome (prosome, macropain) subunit, alpha type, 6 5154 +/- 216 4141 +/- 221 4713 +/- 143 4252 +/- 182 -0.00831829 -0.081581543 0.13117447 0.020298228 PTBP2 polypyrimidine tract binding protein 2 2307 +/- 103 1782 +/- 161 2298 +/- 39 1811 +/- 152 -0.02381853 -0.030830317 0.438613881 0.216102612 PUM2 pumilio homolog 2 (Drosophila) 2633 +/- 77 1935 +/- 111 2753 +/- 131 1997 +/- 141 -0.000604458 -0.003648263 0.05562315 0.081645211 PVRL1 poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin) 255 +/- 18 185 +/- 22 246 +/- 25 246 +/- 12 -0.03258425 0.995348068 0.072251382 0.79964555 RAE1 RAE1 RNA export 1 homolog (S. pombe) 1175 +/- 43 1040 +/- 42 1125 +/- 36 1035 +/- 26 -0.048285977 -0.073808401 0.063972993 0.34033863 RALGPS2 Ral GEF with PH domain and SH3 binding motif 2 280 +/- 27 193 +/- 19 326 +/- 37 295 +/- 16 -0.026245547 -0.484098917 0.24249743 0.660598525 RASGRF1 Ras protein-specific guanine nucleotide-releasing factor 1 125 +/- 16 76 +/- 11 145 +/- 12 104 +/- 10 -0.031471125 -0.025192212 0.075248629 0.025369786 RBM19 RNA binding motif protein 19 727 +/- 55 514 +/- 46 537 +/- 38 381 +/- 60 -0.014212352 -0.062673075 0.075679997 0.198486376 RFT1 RFT1 homolog (S. cerevisiae) 144 +/- 6 123 +/- 7 159 +/- 9 123 +/- 5 -0.053411546 -0.009489794 0.11623316 0.018399732 RIT2 Ras-like without CAAX 2 609 +/- 31 437 +/- 55 775 +/- 62 568 +/- 48 -0.025575445 -0.027875631 0.077862913 0.062894532 RNF10 ring finger protein 10 3235 +/- 192 2478 +/- 121 3155 +/- 83 2677 +/- 130 -0.009517874 -0.017449864 0.082708791 0.409322341 RNF40 ring finger protein 40 697 +/- 31 616 +/- 10 876 +/- 27 750 +/- 31 -0.043586854 -0.014864958 0.340172388 0.108042495 RPL6 ribosomal protein L6 10615 +/- 183 9845 +/- 191 10876 +/- 273 9843 +/- 437 -0.015594257 -0.085700023 0.060213935 0.312322385 RPS12 ribosomal protein S12 4329 +/- 211 3150 +/- 239 3422 +/- 186 2508 +/- 191 -0.004218543 -0.00763633 0.093213947 0.150396701 RPS25 ribosomal protein S25 403 +/- 49 270 +/- 30 272 +/- 35 279 +/- 26 -0.049243212 0.872730942 0.063496005 0.92847741 SBK1 SH3-binding domain kinase 1 898 +/- 90 609 +/- 68 744 +/- 48 642 +/- 28 -0.029437261 -0.101300628 0.057854755 0.575062095 SDCCAG8 serologically defined colon cancer antigen 8 262 +/- 8 225 +/- 12 276 +/- 23 239 +/- 4 -0.027839426 -0.167449419 0.102992468 0.362649026 SEC14L1 SEC14-like 1 (S. cerevisiae) 4771 +/- 153 4085 +/- 221 5018 +/- 284 3990 +/- 299 -0.031449238 -0.03470786 0.459700401 0.261595307

S4- MSI correlated and non-correlated ARGs.xls Page 32 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG SEC22A SEC22 vesicle trafficking protein homolog A (S. cerevisiae) 388 +/- 11 342 +/- 16 440 +/- 19 453 +/- 44 -0.037119823 0.80456365 0.189501618 0.161012479 SEH1L SEH1-like (S. cerevisiae) 297 +/- 24 216 +/- 13 286 +/- 18 233 +/- 18 -0.018529728 -0.067982475 0.087554376 0.032309737 SETD6 SET domain containing 6 357 +/- 14 262 +/- 22 362 +/- 8 285 +/- 23 -0.006155276 -0.024760878 0.071339234 0.157550143 SFRS7 splicing factor, arginine/serine-rich 7, 35kDa 2011 +/- 79 1722 +/- 78 1994 +/- 57 1707 +/- 55 -0.026627131 -0.005697307 0.056949117 0.011437644 SGPL1 sphingosine-1-phosphate lyase 1 613 +/- 28 522 +/- 26 640 +/- 24 524 +/- 12 -0.040205869 -0.002866218 0.231950816 0.057885563 SH3GL1 SH3-domain GRB2-like 1 517 +/- 28 419 +/- 30 649 +/- 35 550 +/- 32 -0.037857085 -0.069011192 0.062625953 0.434473869 SMU1 Smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) 1021 +/- 44 845 +/- 49 1000 +/- 21 892 +/- 31 -0.024100861 -0.021557898 0.061954055 0.167536525 SNX3 sorting nexin 3 14461 +/- 818 11436 +/- 483 13459 +/- 458 11655 +/- 653 -0.012676168 -0.055871839 0.068929772 0.41830052 SORBS2 Sorbin and SH3 domain containing 2 432 +/- 19 353 +/- 14 405 +/- 14 294 +/- 41 -0.007594933 -0.049321265 0.078440767 0.364208937 SOX1 SRY (sex determining region Y)-box 1 375 +/- 39 272 +/- 18 455 +/- 28 336 +/- 23 -0.050160547 -0.010004196 0.137008855 0.008571145 SPA17 sperm autoantigenic protein 17 537 +/- 65 330 +/- 45 443 +/- 23 329 +/- 17 -0.027597301 -0.0033444 0.094748997 0.03123644 SPTBN1 spectrin, beta, non-erythrocytic 1 1270 +/- 153 835 +/- 88 1365 +/- 200 1056 +/- 161 -0.038682956 -0.260651655 0.148603624 0.490188728 SRrp35 serine-arginine repressor protein (35 kDa) 851 +/- 61 650 +/- 57 860 +/- 61 692 +/- 42 -0.036873369 -0.05114665 0.063738218 0.19318906 SSB Sjogren syndrome antigen B (autoantigen La) 2114 +/- 118 1777 +/- 99 2359 +/- 70 1882 +/- 138 -0.054697646 -0.021627807 0.061261408 0.162029076 STK11IP serine/threonine kinase 11 interacting protein 832 +/- 22 692 +/- 33 760 +/- 43 675 +/- 37 -0.007128495 -0.167253036 0.220170601 0.617636968 SUB1 SUB1 homolog (S. cerevisiae) 198 +/- 12 153 +/- 13 233 +/- 7 187 +/- 11 -0.029380853 -0.009334405 0.070572835 0.200849812 SURF4 surfeit 4 844 +/- 29 730 +/- 19 751 +/- 33 661 +/- 21 -0.010483129 -0.048622922 0.102185092 0.242072965 TBC1D14 TBC1 domain family, member 14 2125 +/- 84 1815 +/- 92 1795 +/- 52 1639 +/- 43 -0.031696434 -0.047244724 0.222267241 0.2088684 TCF4 transcription factor 4 4852 +/- 158 4083 +/- 243 6014 +/- 463 4769 +/- 717 -0.027614162 -0.187715991 0.383734576 0.930580376 TGM2 transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) 141 +/- 8 82 +/- 19 137 +/- 20 132 +/- 28 -0.026135081 -0.900788131 0.249611034 0.855752417 TMEM132B transmembrane protein 132B 1274 +/- 123 930 +/- 71 1051 +/- 67 730 +/- 54 -0.041704541 -0.004741841 0.240402673 0.156696458 TMF1 TATA element modulatory factor 1 2030 +/- 133 1560 +/- 130 2148 +/- 200 1684 +/- 181 -0.029535359 -0.118837708 0.063449444 0.093234516 TOR3A torsin family 3, member A 298 +/- 17 220 +/- 15 308 +/- 11 206 +/- 13 -0.006223971 -0.000302833 0.067683608 0.001767536 TRA2A transformer-2 alpha 1956 +/- 84 1639 +/- 102 2458 +/- 104 1919 +/- 80 -0.038510101 -0.002776761 0.316371608 0.022918967 TRAM2 translocation associated membrane protein 2 242 +/- 13 139 +/- 24 223 +/- 20 171 +/- 18 -0.0059956 -0.081751721 0.055881213 0.108538562 TTC19 tetratricopeptide repeat domain 19 2924 +/- 101 1559 +/- 359 3189 +/- 138 1757 +/- 528 -0.011318191 -0.051446777 0.065680723 0.168026491 TXNDC9 thioredoxin domain containing 9 1042 +/- 34 872 +/- 30 979 +/- 44 900 +/- 61 -0.003787137 -0.325892684 0.06940985 0.275006362 USP15 ubiquitin specific peptidase 15 2158 +/- 53 1639 +/- 107 2009 +/- 51 1697 +/- 92 -0.003134811 -0.023252573 0.058322123 0.324435591 UTP18 UTP18, small subunit (SSU) processome component, homolog (yeast) 599 +/- 58 413 +/- 38 673 +/- 50 479 +/- 70 -0.026657729 -0.055462041 0.136250372 0.365674261 VAPA VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa 11082 +/- 381 9798 +/- 151 10132 +/- 197 8992 +/- 317 -0.018084373 -0.018773793 0.114900856 0.004102026 VPS13B vacuolar protein sorting 13B (yeast) 993 +/- 26 852 +/- 41 949 +/- 24 938 +/- 47 -0.018653886 -0.831077605 0.195255498 0.508974452 WAS Wiskott-Aldrich syndrome (eczema-thrombocytopenia) 214 +/- 11 177 +/- 8 239 +/- 10 176 +/- 17 -0.021343083 -0.016448585 0.11705994 0.003993011 WDR23 WD repeat domain 23 428 +/- 44 312 +/- 19 348 +/- 33 318 +/- 26 -0.046415226 -0.501503684 0.07550888 0.624083408 YTHDF2 YTH domain family, member 2 2059 +/- 97 1665 +/- 58 2492 +/- 66 2178 +/- 98 -0.007866082 -0.031414013 0.112830832 0.432848963 ZBTB24 zinc finger and BTB domain containing 24 626 +/- 63 454 +/- 23 608 +/- 38 435 +/- 38 -0.039583377 -0.01011329 0.187721069 0.150985666 ZBTB7A zinc finger and BTB domain containing 7A 1920 +/- 64 1641 +/- 79 2555 +/- 279 2188 +/- 127 -0.021473403 -0.27095963 0.11082624 0.597775134 ZC3H14 zinc finger CCCH-type containing 14 1650 +/- 138 1245 +/- 70 1782 +/- 55 1603 +/- 67 -0.033219408 -0.072997615 0.084289326 0.475887219 ZNF238 zinc finger protein 238 7398 +/- 390 6014 +/- 471 8244 +/- 127 6592 +/- 730 -0.047964266 -0.085760354 0.152232274 0.530061474 ZNF592 zinc finger protein 592 288 +/- 9 242 +/- 16 283 +/- 13 268 +/- 16 -0.040745626 -0.501621816 0.141726982 0.577943699 ZNF74 zinc finger protein 74 147 +/- 15 91 +/- 13 170 +/- 12 113 +/- 14 -0.018936105 -0.016370007 0.112743819 0.206491307 ZNRD1 zinc ribbon domain containing 1 926 +/- 41 625 +/- 37 853 +/- 26 720 +/- 30 -0.000268689 -0.009907701 0.05962573 0.099213787 ZNRF1 zinc and ring finger 1 184 +/- 15 139 +/- 13 202 +/- 22 172 +/- 11 -0.045444127 -0.273991269 0.087313118 0.276516176 ZRANB3 zinc finger, RAN-binding domain containing 3 569 +/- 41 434 +/- 28 513 +/- 24 455 +/- 29 -0.024725059 -0.165970594 0.134178585 0.524079369 ZXDB zinc finger, X-linked, duplicated B 667 +/- 61 496 +/- 39 665 +/- 46 479 +/- 61 -0.044558801 -0.041047339 0.260164736 0.248674131

S4- MSI correlated and non-correlated ARGs.xls Page 33 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG Upregulated with Age in DG, not MSI Correlated AARSL alanyl-tRNA synthetase like 672 +/- 50 780 +/- 24 666 +/- 26 777 +/- 38 0.090418925 0.046839926 0.009757478 0.092213728 ACAA2 acetyl-Coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-Coenzyme A thiolase) 589 +/- 56 854 +/- 62 529 +/- 31 645 +/- 33 0.009788915 0.03015292 0.000593989 0.107868689 ACP6 acid phosphatase 6, lysophosphatidic 881 +/- 49 984 +/- 51 742 +/- 28 837 +/- 8 0.171551013 0.018151246 0.035515858 0.071011219 ACTC actin, alpha, cardiac muscle 84 +/- 15 206 +/- 35 98 +/- 11 212 +/- 35 0.015564453 0.02973628 0.098010841 0.165795281 ACTR10 actin-related protein 10 homolog (S. cerevisiae) 6172 +/- 108 6822 +/- 248 6301 +/- 210 7510 +/- 229 0.048300301 0.003988195 0.590192181 0.084527105 ACY1 aminoacylase 1 493 +/- 28 523 +/- 20 435 +/- 30 520 +/- 22 0.397211309 0.04872429 0.606441409 0.155182979 ADAM10 ADAM metallopeptidase domain 10 1557 +/- 99 1808 +/- 84 1420 +/- 70 1612 +/- 46 0.083712709 0.049117131 0.062791592 0.364935472 ADCK4 aarF domain containing kinase 4 430 +/- 24 546 +/- 40 383 +/- 31 503 +/- 38 0.037411918 0.039519137 0.273091814 0.306370996 ADD3 adducin 3 (gamma) 2311 +/- 330 3058 +/- 364 3069 +/- 265 3733 +/- 91 0.159837408 0.054267015 0.156960222 0.272186304 AHCYL1 S-adenosylhomocysteine hydrolase-like 1 5935 +/- 600 7815 +/- 704 6059 +/- 511 8209 +/- 330 0.07026454 0.007298966 0.178800998 0.194904583 AIM1 absent in melanoma 1 368 +/- 97 238 +/- 44 114 +/- 15 175 +/- 22 -0.26550879 0.050898392 0.416903708 0.355564467 AKR7A2 aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) 1175 +/- 77 1379 +/- 69 853 +/- 46 1153 +/- 50 0.078149443 0.001840641 0.307065365 0.089284605 ALDH3A1 aldehyde dehydrogenase 3 family, memberA1 99 +/- 11 267 +/- 57 115 +/- 22 357 +/- 54 0.031714621 0.008136205 0.140583247 0.129873586 AMY1A amylase, alpha 1A; salivary 217 +/- 46 706 +/- 123 210 +/- 34 692 +/- 108 0.008648671 0.00879563 0.208529984 0.155361317 ANAPC11 APC11 anaphase promoting complex subunit 11 homolog (yeast) 1418 +/- 65 1478 +/- 46 1036 +/- 22 1185 +/- 40 0.465122624 0.015792697 0.960143563 0.259395397 ANKH ankylosis, progressive homolog (mouse) 2365 +/- 119 2794 +/- 121 2527 +/- 92 2834 +/- 84 0.030335857 0.035777518 0.166489153 0.306504297 ANKRD10 ankyrin repeat domain 10 912 +/- 51 1028 +/- 46 829 +/- 38 1027 +/- 65 0.11682503 0.035518672 0.166515558 0.216663444 ANKRD16 ankyrin repeat domain 16 117 +/- 11 155 +/- 15 124 +/- 12 175 +/- 8 0.078602961 0.005667174 0.409956627 0.082437628 ANKS1B ankyrin repeat and sterile alpha motif domain containing 1B 7067 +/- 478 8852 +/- 493 7058 +/- 279 8534 +/- 485 0.026588622 0.035708506 0.007534706 0.11313409 APLP1 amyloid beta (A4) precursor-like protein 1 12980 +/- 687 13451 +/- 820 11777 +/- 440 13170 +/- 402 0.669959939 0.04423183 0.621193395 0.203239234 ARHGAP1 Rho GTPase activating protein 1 2732 +/- 133 3160 +/- 91 2618 +/- 114 2959 +/- 91 0.026130162 0.045361642 0.13775446 0.424521136 ASTN2 Astrotactin 2 149 +/- 10 185 +/- 27 178 +/- 20 260 +/- 21 0.254061804 0.021934515 0.499386651 0.285792225 AURKAIP1 aurora kinase A interacting protein 1 1086 +/- 137 1595 +/- 169 1097 +/- 103 1678 +/- 137 0.042673648 0.009991978 0.163010822 0.071334126 AYP1 AYP1 protein 1952 +/- 156 2320 +/- 203 1716 +/- 121 2044 +/- 75 0.183417124 0.04991638 0.080897619 0.067434789 B2M beta-2-microglobulin 7946 +/- 519 10622 +/- 678 6616 +/- 572 9837 +/- 646 0.011496581 0.005147291 0.04236259 0.105180339 BAG5 BCL2-associated athanogene 5 1207 +/- 46 1153 +/- 44 1176 +/- 30 1285 +/- 20 -0.417450154 0.015127155 0.443112885 0.078735196 BAX BCL2-associated X protein 831 +/- 23 877 +/- 24 728 +/- 13 807 +/- 21 0.188443016 0.016139953 0.345293714 0.160529309 BBX bobby sox homolog (Drosophila) 1408 +/- 90 1558 +/- 89 1131 +/- 18 1251 +/- 43 0.263537884 0.045522952 0.125772394 0.099866251 BTBD2 BTB (POZ) domain containing 2 1567 +/- 138 1823 +/- 101 1620 +/- 100 2016 +/- 105 0.168753944 0.02356382 0.450145408 0.068602553 BZRP benzodiazapine receptor (peripheral) 565 +/- 74 798 +/- 62 374 +/- 21 504 +/- 36 0.037740141 0.018487277 0.021493421 0.101408274 CALCOCO1 calcium binding and coiled-coil domain 1 1651 +/- 65 1696 +/- 88 1450 +/- 9 1669 +/- 54 0.694453646 0.014306965 0.314744804 0.174343623 CAPN2 calpain 2, (m/II) large subunit 2440 +/- 204 3016 +/- 132 1931 +/- 137 2492 +/- 70 0.043050555 0.007570247 0.056262271 0.076747121 CBX3 chromobox homolog 3 (HP1 gamma homolog, Drosophila) 2091 +/- 138 2460 +/- 88 2478 +/- 74 2807 +/- 90 0.052848432 0.02160915 0.09765007 0.057418071 CCDC28A coiled-coil domain containing 28A 2372 +/- 140 2508 +/- 155 2361 +/- 84 2730 +/- 55 0.529130404 0.005847384 0.829814971 0.086578412 CCDC46 coiled-coil domain containing 46 98 +/- 13 159 +/- 31 208 +/- 13 282 +/- 17 0.119453512 0.009055816 0.252766594 0.156967942 CCNDBP1 cyclin D-type binding-protein 1 1466 +/- 70 1579 +/- 106 1288 +/- 57 1562 +/- 89 0.396873328 0.03535486 0.750233003 0.138580425 CCNF cyclin F 133 +/- 13 125 +/- 9 143 +/- 17 196 +/- 13 -0.62394445 0.034926043 0.366478102 0.148804608 CCT3 chaperonin containing TCP1, subunit 3 (gamma) 2068 +/- 138 2872 +/- 102 1999 +/- 97 2727 +/- 72 0.001085379 0.000217763 0.075896541 0.061884585 CD63 CD63 molecule 3961 +/- 565 4903 +/- 297 3173 +/- 139 3664 +/- 165 0.180362566 0.050995783 0.198091984 0.403602399 CD74 CD74 molecule, major histocompatibility complex, class II invariant chain 1810 +/- 283 3714 +/- 547 1215 +/- 171 2317 +/- 340 0.016152974 0.027628935 0.007913266 0.104936148 CD81 CD81 molecule 13811 +/- 551 15425 +/- 255 12732 +/- 477 14927 +/- 743 0.032375137 0.041802505 0.194433538 0.42501107 CDC42EP1 CDC42 effector protein (Rho GTPase binding) 1 483 +/- 70 667 +/- 95 446 +/- 30 548 +/- 33 0.150233392 0.047947836 0.072241102 0.096734793 CDK5RAP3 CDK5 regulatory subunit associated protein 3 2040 +/- 139 2326 +/- 68 1983 +/- 100 2344 +/- 76 0.105901228 0.018857844 0.276131429 0.128142931

S4- MSI correlated and non-correlated ARGs.xls Page 34 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG CEP170 centrosomal protein 170kDa 1001 +/- 123 1338 +/- 117 981 +/- 72 1209 +/- 49 0.075320614 0.029524785 0.13195757 0.346627728 CFL2 cofilin 2 (muscle) 1198 +/- 120 1807 +/- 334 1128 +/- 106 1503 +/- 117 0.135085777 0.042518055 0.017425991 0.065838282 CGI-38 brain specific protein 1185 +/- 160 1693 +/- 328 699 +/- 44 901 +/- 69 0.204303732 0.043389745 0.172850293 0.13587124 CKS1B CDC28 protein kinase regulatory subunit 1B 822 +/- 24 945 +/- 35 686 +/- 28 763 +/- 14 0.018041856 0.041455361 0.045112677 0.247541605 CMTM3 CKLF-like MARVEL transmembrane domain containing 3 684 +/- 73 783 +/- 21 553 +/- 20 645 +/- 18 0.241348718 0.007505725 0.18807065 0.06727828 CMTM5 CKLF-like MARVEL transmembrane domain containing 5 2631 +/- 293 3487 +/- 483 1346 +/- 59 1861 +/- 191 0.166951238 0.051692549 0.069622957 0.070919054 CNTN1 contactin 1 1106 +/- 69 1435 +/- 87 1088 +/- 58 1302 +/- 69 0.014910358 0.04357938 0.008978749 0.206808102 COPB coatomer protein complex, subunit beta 2361 +/- 106 2533 +/- 108 2354 +/- 49 2670 +/- 101 0.279401813 0.030976886 0.641905093 0.211013543 COPS7A COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis) 2132 +/- 50 2760 +/- 207 2135 +/- 183 2910 +/- 174 0.027680484 0.01351365 0.352985886 0.252014955 COQ3 coenzyme Q3 homolog, methyltransferase (S. cerevisiae) 962 +/- 18 1137 +/- 65 967 +/- 22 1098 +/- 25 0.043000068 0.00382441 0.334787333 0.133082872 COTL1 coactosin-like 1 (Dictyostelium) 2231 +/- 123 2629 +/- 248 2186 +/- 97 2676 +/- 151 0.192130519 0.029099849 0.150606173 0.080539828 COX4NB COX4 neighbor 1259 +/- 72 1292 +/- 57 1207 +/- 23 1340 +/- 34 0.725012669 0.012695773 0.985407772 0.273186101 COX5B cytochrome c oxidase subunit Vb 6825 +/- 788 9221 +/- 528 4736 +/- 885 7292 +/- 122 0.033108929 0.03387958 0.066557107 0.06557043 COX6C cytochrome c oxidase subunit VIc 7080 +/- 300 8976 +/- 497 6419 +/- 237 8142 +/- 260 0.011021066 0.0009592 0.204294097 0.061635335 CRNKL1 Crn, crooked neck-like 1 (Drosophila) 267 +/- 89 512 +/- 56 270 +/- 68 595 +/- 45 0.046427355 0.003682882 0.102437924 0.059562553 CTSL cathepsin L 1295 +/- 116 1543 +/- 70 1117 +/- 86 1413 +/- 26 0.102404334 0.017269758 0.217904315 0.182779481 CYCS cytochrome c, somatic 1114 +/- 69 1523 +/- 105 1124 +/- 93 1485 +/- 52 0.010341606 0.010190902 0.183603931 0.132190411 D21S2056E DNA segment on chromosome 21 (unique) 2056 expressed sequence 941 +/- 105 1138 +/- 59 786 +/- 44 1023 +/- 84 0.140487651 0.046019942 0.259928769 0.169810671 DDB2 damage-specific DNA binding protein 2, 48kDa 98 +/- 11 160 +/- 9 88 +/- 9 129 +/- 8 0.001531774 0.005825251 0.002332177 0.056883278 DDX48 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 1505 +/- 88 1696 +/- 45 1343 +/- 72 1561 +/- 54 0.091721379 0.038877818 0.021703627 0.22882604 DIP13B DIP13 beta 720 +/- 55 858 +/- 45 786 +/- 23 921 +/- 33 0.082075834 0.010296974 0.121643478 0.266151183 DNAJB4 DnaJ (Hsp40) homolog, subfamily B, member 4 249 +/- 44 273 +/- 40 286 +/- 16 415 +/- 45 0.688849593 0.042425719 0.654877194 0.144355522 DNM2 dynamin 2 623 +/- 66 744 +/- 63 541 +/- 14 626 +/- 29 0.214331217 0.040640265 0.145555683 0.079007191 DOCK4 dedicator of cytokinesis 4 83 +/- 24 256 +/- 47 145 +/- 31 379 +/- 38 0.011861311 0.00134105 0.068950788 0.102620985 DRG2 developmentally regulated GTP binding protein 2 573 +/- 52 606 +/- 35 521 +/- 31 660 +/- 42 0.616675727 0.03142674 0.900796063 0.174145099 DUS3L dihydrouridine synthase 3-like (S. cerevisiae) 283 +/- 16 306 +/- 11 221 +/- 15 291 +/- 20 0.273664984 0.02486242 0.371895688 0.144701876 EDG7 Endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor, 7 906 +/- 85 1108 +/- 56 920 +/- 115 1256 +/- 85 0.078961072 0.044671982 0.054416416 0.160917877 EEF1A1 eukaryotic translation elongation factor 1 alpha 1 11705 +/- 649 13512 +/- 606 11114 +/- 370 12852 +/- 588 0.069427744 0.041287408 0.345674342 0.491407587 EFEMP1 EGF-containing fibulin-like extracellular matrix protein 1 5116 +/- 149 5852 +/- 332 4920 +/- 274 5811 +/- 259 0.086310426 0.042474705 0.022277269 0.155373464 EFHC2 EF-hand domain (C-terminal) containing 2 234 +/- 19 266 +/- 17 174 +/- 10 237 +/- 7 0.235677986 0.000754633 0.37893931 0.064217335 EGLN2 egl nine homolog 2 (C. elegans) 198 +/- 34 157 +/- 24 231 +/- 12 268 +/- 10 -0.343569739 0.043260351 0.445130998 0.416431218 EID-3 EID-2-like inhibitor of differentiation-3 1170 +/- 82 1234 +/- 63 1297 +/- 45 1499 +/- 44 0.551202192 0.010925624 0.817641259 0.087902694 EIF5B eukaryotic translation initiation factor 5B 746 +/- 19 848 +/- 36 734 +/- 20 791 +/- 15 0.037126904 0.052223377 0.002047749 0.055285422 EMD emerin (Emery-Dreifuss muscular dystrophy) 883 +/- 36 950 +/- 27 898 +/- 19 978 +/- 23 0.170861884 0.02664236 0.727309209 0.383579834 ENO1 enolase 1, (alpha) 7846 +/- 333 9198 +/- 336 6480 +/- 135 7880 +/- 253 0.016967227 0.002519542 0.07839016 0.056871124 EPHB6 EPH receptor B6 1193 +/- 220 1171 +/- 59 814 +/- 60 1044 +/- 67 -0.925674876 0.032398906 0.797284305 0.163483564 ERGIC3 ERGIC and golgi 3 2303 +/- 107 2433 +/- 73 2054 +/- 51 2319 +/- 42 0.342528869 0.003142398 0.817773393 0.111969481 FARP1 FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) 387 +/- 68 1140 +/- 179 336 +/- 59 910 +/- 114 0.006669617 0.004168685 0.139806747 0.091282321 FN3KRP fructosamine-3-kinase-related protein 2327 +/- 186 2932 +/- 222 2878 +/- 211 3527 +/- 36 0.0643649 0.027114425 0.247655812 0.095468877 FSTL1 follistatin-like 1 1444 +/- 104 1350 +/- 62 956 +/- 42 1093 +/- 20 -0.458444517 0.021569149 0.493827998 0.143663151 FTL ferritin, light polypeptide 16238 +/- 1033 18500 +/- 704 14150 +/- 408 17258 +/- 618 0.104527143 0.003849721 0.112985505 0.102660672 FYCO1 FYVE and coiled-coil domain containing 1 428 +/- 37 875 +/- 195 355 +/- 20 531 +/- 43 0.07053237 0.011509937 0.119591693 0.117650544 GABARAP GABA(A) receptor-associated protein 11702 +/- 341 11960 +/- 190 10752 +/- 157 11400 +/- 130 0.528219608 0.011298876 0.558481851 0.063827617 GINS1 GINS complex subunit 1 (Psf1 homolog) 2077 +/- 122 2623 +/- 181 2094 +/- 32 2596 +/- 160 0.034320522 0.033856266 0.367407227 0.345335418

S4- MSI correlated and non-correlated ARGs.xls Page 35 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG GJA12 gap junction protein, alpha 12, 47kDa 683 +/- 86 921 +/- 117 493 +/- 44 660 +/- 35 0.135739642 0.01554807 0.090239701 0.124677578 GLDN gliomedin 316 +/- 58 508 +/- 25 308 +/- 25 409 +/- 21 0.019579409 0.013554132 0.027041741 0.13033409 GNB2L1 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 8227 +/- 314 9584 +/- 311 7947 +/- 439 9269 +/- 228 0.011821474 0.030345679 0.037887298 0.095970664 GPC4 glypican 4 206 +/- 17 250 +/- 35 346 +/- 28 436 +/- 14 0.288748704 0.023211549 0.655360395 0.210196686 GPM6B glycoprotein M6B 5149 +/- 133 6879 +/- 254 6195 +/- 386 7340 +/- 323 0.000392904 0.049273197 0.007491822 0.472334046 GRHPR glyoxylate reductase/hydroxypyruvate reductase 1359 +/- 124 1733 +/- 104 1291 +/- 74 1730 +/- 83 0.044182681 0.003678999 0.22865394 0.068657893 GSN gelsolin (amyloidosis, Finnish type) 4180 +/- 541 5269 +/- 560 2845 +/- 206 3577 +/- 214 0.192734399 0.0363701 0.233600135 0.13201493 GUP1 GUP1 glycerol uptake/transporter homolog (S. cerevisiae) 602 +/- 64 1060 +/- 102 467 +/- 55 753 +/- 60 0.004636206 0.006825919 0.00498831 0.056779731 HDAC11 Histone deacetylase 11 3200 +/- 431 3729 +/- 400 2319 +/- 144 2838 +/- 131 0.389408521 0.025646011 0.258166221 0.070318402 HDAC6 histone deacetylase 6 858 +/- 46 1082 +/- 96 763 +/- 14 907 +/- 33 0.072679649 0.008625125 0.240840634 0.076849185 HIGD1B HIG1 domain family, member 1B 225 +/- 13 268 +/- 11 154 +/- 8 203 +/- 12 0.030329094 0.009652683 0.032430077 0.057293297 HMG2L1 high-mobility group protein 2-like 1 827 +/- 48 1078 +/- 75 788 +/- 50 931 +/- 40 0.020869135 0.052828578 0.005334313 0.098226025 HSD11B1 hydroxysteroid (11-beta) dehydrogenase 1 182 +/- 39 214 +/- 25 78 +/- 15 136 +/- 13 0.503279126 0.015016412 0.586189179 0.060650646 HSPB8 heat shock 22kDa protein 8 2202 +/- 213 3231 +/- 299 2037 +/- 225 2797 +/- 223 0.020507528 0.040315812 0.080176878 0.384594436 IBRDC3 IBR domain containing 3 179 +/- 43 292 +/- 22 217 +/- 41 344 +/- 26 0.047629109 0.029838298 0.219191824 0.299550357 ICAM1 intercellular adhesion molecule 1 (CD54), human rhinovirus receptor 120 +/- 10 185 +/- 13 126 +/- 9 167 +/- 8 0.002775713 0.007973606 0.00122463 0.186165299 IFNAR2 interferon (alpha, beta and omega) receptor 2 189 +/- 22 286 +/- 18 155 +/- 25 256 +/- 36 0.007188099 0.051828412 0.04034989 0.129988797 IFNGR1 interferon gamma receptor 1 1775 +/- 52 2011 +/- 102 1350 +/- 54 1527 +/- 56 0.075620617 0.049648095 0.031399147 0.095448484 IGF2 insulin-like growth factor 2 (somatomedin A) 536 +/- 149 327 +/- 80 242 +/- 33 429 +/- 64 -0.252465811 0.040788384 0.113397395 0.115069891 IGSF11 immunoglobulin superfamily, member 11 1835 +/- 127 2330 +/- 191 1702 +/- 60 1916 +/- 70 0.060224614 0.04837478 0.012762648 0.092311293 INSIG1 insulin induced gene 1 1796 +/- 160 2510 +/- 276 2251 +/- 175 2909 +/- 224 0.055464099 0.049264887 0.082813206 0.406367142 ISG15 ISG15 ubiquitin-like modifier 312 +/- 25 375 +/- 30 216 +/- 17 298 +/- 16 0.142738312 0.007131799 0.108519497 0.204302163 ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) 657 +/- 64 790 +/- 95 641 +/- 35 744 +/- 25 0.281100272 0.041142204 0.113348138 0.078999066 ITGB4 integrin, beta 4 433 +/- 101 626 +/- 98 854 +/- 90 1205 +/- 70 0.199322537 0.013636801 0.311950499 0.128101843 ITM2A integral membrane protein 2A 1463 +/- 86 1990 +/- 49 1400 +/- 32 1806 +/- 148 0.000728073 0.050000797 0.020938239 0.247782417 KIF3A kinesin family member 3A 6022 +/- 351 7526 +/- 490 5756 +/- 353 7952 +/- 415 0.033884807 0.003445953 0.447769441 0.104240769 KIF5A kinesin family member 5A 2488 +/- 627 4967 +/- 527 2216 +/- 519 3839 +/- 250 0.013155403 0.025515348 0.069802094 0.168159715 KRR1 KRR1, small subunit (SSU) processome component, homolog (yeast) 143 +/- 22 372 +/- 77 168 +/- 37 387 +/- 79 0.030300603 0.048322189 0.282459063 0.327733661 LAYN layilin 174 +/- 39 137 +/- 9 89 +/- 5 120 +/- 9 -0.392233427 0.018567123 0.164778022 0.228966628 LGMN legumain 2017 +/- 149 2333 +/- 156 1847 +/- 52 2339 +/- 106 0.175161521 0.00607512 0.455152068 0.133507269 LHPP phospholysine phosphohistidine inorganic pyrophosphate phosphatase 1897 +/- 163 2175 +/- 138 1606 +/- 105 1935 +/- 93 0.223221226 0.043901892 0.142317166 0.217039603 LONPL peroxisomal LON protease like 691 +/- 82 856 +/- 45 737 +/- 44 891 +/- 39 0.11548954 0.02841048 0.079130996 0.076752512 LYK5 protein kinase LYK5 1182 +/- 41 1209 +/- 34 1201 +/- 21 1266 +/- 17 0.622556777 0.038831136 0.540476426 0.066031787 MAD2L2 MAD2 mitotic arrest deficient-like 2 (yeast) 622 +/- 72 573 +/- 24 543 +/- 12 601 +/- 20 -0.546858762 0.044277451 0.497512467 0.209700279 MAN2A1 Mannosidase, alpha, class 2A, member 1 1559 +/- 217 2028 +/- 286 855 +/- 94 1116 +/- 57 0.221928283 0.044273356 0.18788158 0.143701298 MAP3K13 Mitogen-activated protein kinase kinase kinase 13 655 +/- 36 806 +/- 40 539 +/- 37 736 +/- 36 0.019233825 0.004277603 0.193007684 0.08807373 MCCC1 methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) 588 +/- 18 799 +/- 27 614 +/- 25 820 +/- 25 0.000133926 0.00024902 0.025550241 0.064593462 MEGF10 multiple EGF-like-domains 10 775 +/- 88 1122 +/- 99 648 +/- 44 768 +/- 24 0.025981771 0.045695659 0.012992723 0.233386867 METTL5 methyltransferase like 5 537 +/- 35 732 +/- 38 613 +/- 18 740 +/- 26 0.003613606 0.004300567 0.06472819 0.139529211 MID1IP1 MID1 interacting protein 1 (gastrulation specific G12-like (zebrafish)) 3282 +/- 363 4184 +/- 464 3175 +/- 236 3893 +/- 110 0.158427813 0.02813132 0.055935797 0.067190839 MOBP myelin-associated oligodendrocyte basic protein 3329 +/- 750 6661 +/- 1634 1660 +/- 465 3347 +/- 458 0.106168045 0.029746903 0.016324371 0.05734512 MRFAP1 Mof4 family associated protein 1 14404 +/- 697 16399 +/- 660 13724 +/- 840 16337 +/- 665 0.064453558 0.03776177 0.052246116 0.064904074 MRPS15 mitochondrial ribosomal protein S15 1027 +/- 36 1142 +/- 68 917 +/- 42 1056 +/- 29 0.173167858 0.023979635 0.119276513 0.105885229 MRPS7 mitochondrial ribosomal protein S7 629 +/- 23 658 +/- 22 625 +/- 13 709 +/- 21 0.388263555 0.011889288 0.749164822 0.119174775

S4- MSI correlated and non-correlated ARGs.xls Page 36 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG MSTO1 misato homolog 1 (Drosophila) 494 +/- 31 687 +/- 43 490 +/- 34 667 +/- 50 0.005029979 0.02122682 0.054397357 0.261320069 MYL6 myosin, light polypeptide 6, alkali, smooth muscle and non-muscle 5381 +/- 175 5499 +/- 158 4330 +/- 103 4685 +/- 116 0.628657669 0.048931037 0.903678889 0.209868003 MYLK myosin, light polypeptide kinase 2425 +/- 309 3340 +/- 241 1446 +/- 132 2443 +/- 131 0.043082979 0.000477748 0.11663479 0.069364667 NARFL nuclear prelamin A recognition factor-like 763 +/- 44 946 +/- 80 767 +/- 41 988 +/- 52 0.081514566 0.010085315 0.264246001 0.134715202 NBR1 neighbor of BRCA1 gene 1 1100 +/- 31 1283 +/- 49 1205 +/- 34 1320 +/- 16 0.012247868 0.018008543 0.001584926 0.086231024 NDUFB2 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa 2656 +/- 38 2528 +/- 107 2217 +/- 39 2426 +/- 38 -0.300336569 0.003892057 0.010258891 0.114578704 NEU4 sialidase 4 318 +/- 28 531 +/- 71 374 +/- 25 540 +/- 33 0.029472306 0.003914097 0.002864159 0.068256338 NFX1 nuclear transcription factor, X-box binding 1 115 +/- 15 162 +/- 12 126 +/- 3 169 +/- 16 0.039838428 0.047698285 0.119763606 0.078436117 NIBP NIK and IKK{beta} binding protein 744 +/- 67 889 +/- 46 598 +/- 42 755 +/- 42 0.109669292 0.026364631 0.160191984 0.164410187 NRP2 neuropilin 2 178 +/- 27 256 +/- 23 279 +/- 17 347 +/- 12 0.054668392 0.010230952 0.044184651 0.056937728 NUDT5 nudix (nucleoside diphosphate linked moiety X)-type motif 5 884 +/- 75 1105 +/- 67 672 +/- 11 954 +/- 63 0.053390579 0.010161315 0.094210476 0.12241466 OBFC1 oligonucleotide/oligosaccharide-binding fold containing 1 224 +/- 12 220 +/- 22 169 +/- 11 211 +/- 12 -0.891074693 0.028949034 0.425395128 0.32643494 OSBPL2 oxysterol binding protein-like 2 588 +/- 28 649 +/- 27 587 +/- 10 638 +/- 14 0.155040087 0.0194728 0.072591055 0.081167639 OSGEP O-sialoglycoprotein endopeptidase 459 +/- 30 536 +/- 32 410 +/- 14 486 +/- 23 0.112129968 0.027452984 0.442568705 0.075976483 PARP8 poly (ADP-ribose) polymerase family, member 8 406 +/- 45 403 +/- 30 443 +/- 29 519 +/- 18 -0.951027906 0.053006248 0.942158742 0.195589884 PCOLN3 procollagen (type III) N-endopeptidase 961 +/- 26 1244 +/- 123 979 +/- 64 1383 +/- 101 0.069583005 0.0121672 0.200868813 0.14737799 PDE8B phosphodiesterase 8B 248 +/- 54 368 +/- 71 377 +/- 54 570 +/- 63 0.207360856 0.045280046 0.661091936 0.398262891 PDIA2 protein disulfide isomerase family A, member 2 137 +/- 34 221 +/- 20 178 +/- 12 237 +/- 20 0.063359719 0.044120945 0.115138431 0.065775794 PDK3 Pyruvate dehydrogenase kinase, isozyme 3 704 +/- 98 960 +/- 111 993 +/- 129 1593 +/- 94 0.113882019 0.004769876 0.428202723 0.064305474 PFDN4 prefoldin subunit 4 737 +/- 71 858 +/- 41 809 +/- 83 1017 +/- 19 0.178896009 0.053248731 0.172067647 0.240539698 PFKL phosphofructokinase, liver 1784 +/- 148 1890 +/- 147 1627 +/- 63 1907 +/- 88 0.61987493 0.033722935 0.896455807 0.112636176 PHF10 PHD finger protein 10 327 +/- 31 419 +/- 15 380 +/- 14 460 +/- 26 0.032516087 0.03490785 0.038848268 0.108541702 PIGS phosphatidylinositol glycan, class S 2823 +/- 73 3263 +/- 183 2696 +/- 71 3307 +/- 125 0.062592984 0.004496106 0.214475852 0.093165484 PIPOX pipecolic acid oxidase 388 +/- 35 493 +/- 32 447 +/- 29 523 +/- 16 0.052153317 0.0513335 0.036558124 0.332666461 PLCH1 phospholipase C, eta 1 327 +/- 12 405 +/- 32 217 +/- 23 304 +/- 19 0.058135757 0.016980964 0.10758693 0.099587986 PLCH2 phospholipase C, eta 2 370 +/- 51 502 +/- 14 513 +/- 63 801 +/- 59 0.049362678 0.008956677 0.067947152 0.087632183 PLEKHA6 pleckstrin homology domain containing, family A member 6 566 +/- 46 738 +/- 53 585 +/- 29 732 +/- 40 0.034289835 0.018921166 0.162664897 0.080752046 PLEKHH1 pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 5498 +/- 891 7056 +/- 1244 2329 +/- 184 3044 +/- 221 0.33509383 0.037095414 0.126982435 0.055861976 PLXNA3 plexin A3 205 +/- 22 318 +/- 36 319 +/- 36 416 +/- 24 0.028119323 0.054836451 0.038607059 0.213522033 PMS2 PMS2 postmeiotic segregation increased 2 (S. cerevisiae) 429 +/- 49 1254 +/- 258 530 +/- 58 1843 +/- 341 0.023149503 0.017204806 0.200908195 0.119468895 POLD2 Polymerase (DNA directed), delta 2, regulatory subunit 50kDa 88 +/- 13 142 +/- 12 136 +/- 20 197 +/- 12 0.01183106 0.028238118 0.079666315 0.256120179 POLG polymerase (DNA directed), gamma 1237 +/- 137 1440 +/- 127 1489 +/- 88 1711 +/- 27 0.303373482 0.053158439 0.188355125 0.116686861 PQLC3 PQ loop repeat containing 3 422 +/- 15 400 +/- 47 254 +/- 11 298 +/- 12 -0.67462566 0.023289474 0.696102953 0.333425692 PRKAB2 protein kinase, AMP-activated, beta 2 non-catalytic subunit 697 +/- 64 885 +/- 68 772 +/- 23 952 +/- 40 0.072252641 0.006776528 0.251662636 0.100464469 PRRG1 proline rich Gla (G-carboxyglutamic acid) 1 909 +/- 109 1222 +/- 166 601 +/- 15 713 +/- 29 0.149903006 0.014550283 0.026593935 0.061201776 PSMA3 proteasome (prosome, macropain) subunit, alpha type, 3 1537 +/- 38 1871 +/- 67 1481 +/- 61 1913 +/- 144 0.002582344 0.03633931 0.047591605 0.162895805 PSME2 proteasome (prosome, macropain) activator subunit 2 (PA28 beta) 821 +/- 80 977 +/- 77 784 +/- 43 921 +/- 39 0.188306895 0.042435257 0.313424352 0.239555764 PTP4A2 protein tyrosine phosphatase type IVA, member 2 6286 +/- 287 7210 +/- 518 5634 +/- 234 7240 +/- 434 0.158091566 0.016418053 0.071542259 0.146304192 PTP4A3 protein tyrosine phosphatase type IVA, member 3 107 +/- 7 143 +/- 10 103 +/- 8 139 +/- 10 0.013215792 0.023195861 0.197196097 0.071717067 PTPRB protein tyrosine phosphatase, receptor type, B 193 +/- 17 229 +/- 15 142 +/- 9 176 +/- 12 0.146745587 0.053788742 0.164375315 0.159282656 RAB32 RAB32, member RAS oncogene family 198 +/- 18 242 +/- 23 137 +/- 8 201 +/- 18 0.165471603 0.020571618 0.170373303 0.121408827 RAB7 RAB7, member RAS oncogene family 9341 +/- 493 9851 +/- 264 7242 +/- 277 8105 +/- 249 0.3893238 0.045657922 0.72490049 0.287858163 RABL4 RAB, member of RAS oncogene family-like 4 740 +/- 95 1128 +/- 110 549 +/- 56 835 +/- 106 0.023895769 0.054057633 0.076826758 0.321291487 RANBP5 RAN binding protein 5 936 +/- 51 1233 +/- 94 1110 +/- 54 1359 +/- 44 0.025319009 0.005955687 0.189239579 0.186509537

S4- MSI correlated and non-correlated ARGs.xls Page 37 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG RANBP6 RAN binding protein 6 782 +/- 58 993 +/- 47 796 +/- 88 1061 +/- 36 0.018602372 0.028990382 0.04712257 0.074729428 RAP80 receptor associated protein 80 1079 +/- 80 1269 +/- 127 891 +/- 21 956 +/- 15 0.24024027 0.03338879 0.377964654 0.130328243 RBM25 RNA binding motif protein 25 1188 +/- 33 1453 +/- 33 1540 +/- 42 1830 +/- 101 0.000207634 0.041768993 0.005200005 0.226285734 RCHY1 ring finger and CHY zinc finger domain containing 1 986 +/- 103 1349 +/- 54 982 +/- 90 1245 +/- 17 0.01521295 0.031787463 0.054679978 0.202165285 RNASE6 ribonuclease, RNase A family, k6 397 +/- 35 654 +/- 73 255 +/- 16 436 +/- 47 0.014909564 0.014505765 0.006242408 0.074681061 RNF130 ring finger protein 130 2915 +/- 322 3688 +/- 372 2157 +/- 119 2608 +/- 105 0.148104173 0.019516522 0.061984765 0.080656052 RNF141 ring finger protein 141 443 +/- 75 613 +/- 119 430 +/- 48 621 +/- 45 0.261835623 0.017611444 0.106035269 0.107064658 RP11-217H1implantation-associated protein 540 +/- 57 800 +/- 92 433 +/- 35 574 +/- 40 0.041470768 0.027972795 0.00427697 0.14838873 RPRC1 arginine/proline rich coiled-coil 1 6598 +/- 221 6480 +/- 243 6871 +/- 111 7286 +/- 69 -0.725680672 0.013168689 0.507847455 0.10427147 RPS24 ribosomal protein S24 19440 +/- 754 21550 +/- 931 17416 +/- 712 21538 +/- 983 0.110193751 0.010136048 0.358614016 0.097505268 RPS6KB1 ribosomal protein S6 kinase, 70kDa, polypeptide 1 1507 +/- 66 2010 +/- 89 1589 +/- 57 1912 +/- 83 0.001300869 0.013806977 0.043269804 0.121311674 S100A6 S100 calcium binding protein A6 (calcyclin) 923 +/- 64 1156 +/- 72 682 +/- 81 1085 +/- 58 0.035962432 0.003149417 0.220246563 0.1084609 SAMD13 sterile alpha motif domain containing 13 234 +/- 23 238 +/- 21 261 +/- 20 314 +/- 8 0.903300686 0.044956191 0.922247198 0.231065771 SC4MOL sterol-C4-methyl oxidase-like 5593 +/- 587 6927 +/- 418 5526 +/- 348 7175 +/- 378 0.097213757 0.011246032 0.241812869 0.087004427 SEL1L sel-1 suppressor of lin-12-like (C. elegans) 350 +/- 53 493 +/- 66 413 +/- 38 592 +/- 39 0.121724193 0.010125779 0.152449292 0.165451398 SELS selenoprotein S 2984 +/- 127 2979 +/- 209 2303 +/- 53 2615 +/- 85 -0.986170037 0.017135682 0.789929202 0.079028255 SERPINB8 serpin peptidase inhibitor, clade B (ovalbumin), member 8 103 +/- 14 142 +/- 10 101 +/- 7 125 +/- 8 0.0451369 0.047732079 0.210402796 0.567889163 SH3BGRL SH3 domain binding glutamic acid-rich protein like 1959 +/- 216 2188 +/- 169 2416 +/- 113 2842 +/- 144 0.422246125 0.048322156 0.305734183 0.272861659 SLC12A9 solute carrier family 12 (potassium/chloride transporters), member 9 1093 +/- 101 1413 +/- 80 1023 +/- 47 1257 +/- 43 0.033133089 0.005050841 0.144496165 0.152042307 SLC25A16 solute carrier family 25 (mitochondrial carrier; Graves disease autoantigen), member 16 472 +/- 27 547 +/- 23 566 +/- 28 661 +/- 17 0.062968315 0.021426681 0.4766455 0.078900085 SLC2A5 solute carrier family 2 (facilitated glucose/fructose transporter), member 5 534 +/- 45 707 +/- 82 446 +/- 26 613 +/- 49 0.102204196 0.0238476 0.12591755 0.289938866 SMG7 Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) 162 +/- 20 141 +/- 13 158 +/- 9 189 +/- 8 -0.403040824 0.024008982 0.495164729 0.225124116 SNAP91 synaptosomal-associated protein, 91kDa homolog (mouse) 10224 +/- 579 10352 +/- 809 10870 +/- 483 12419 +/- 359 0.900359605 0.030730506 0.700578139 0.250164367 SNF8 SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) 2300 +/- 87 2382 +/- 140 1932 +/- 69 2281 +/- 69 0.633700834 0.005860847 0.917105614 0.061040333 SNORA24 Small nucleolar RNA, H/ACA box 24 4609 +/- 197 5659 +/- 543 3508 +/- 255 4534 +/- 188 0.116987217 0.010709051 0.29641677 0.066739239 SNX1 Sorting nexin 1 172 +/- 19 261 +/- 27 175 +/- 13 234 +/- 16 0.024268377 0.020264833 0.013460226 0.242657218 SNX17 sorting nexin 17 2369 +/- 40 2361 +/- 64 1963 +/- 54 2132 +/- 51 -0.920822614 0.05042472 0.208647965 0.384066471 SPCS1 signal peptidase complex subunit 1 homolog (S. cerevisiae) 6159 +/- 178 6206 +/- 222 5341 +/- 222 6203 +/- 226 0.872947371 0.023844644 0.565695968 0.205134747 SPG21 spastic paraplegia 21 (autosomal recessive, Mast syndrome) 2258 +/- 168 2325 +/- 93 1675 +/- 47 1917 +/- 25 0.735305607 0.002343869 0.972420639 0.064318538 SR-A1 serine arginine-rich pre-mRNA splicing factor SR-A1 440 +/- 30 527 +/- 58 660 +/- 33 789 +/- 43 0.220476591 0.046112859 0.815376904 0.105745192 SRD5A1 steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1) 264 +/- 147 539 +/- 62 255 +/- 133 609 +/- 56 0.130382014 0.044720097 0.239776655 0.072801257 SREBF2 sterol regulatory element binding transcription factor 2 1173 +/- 80 1157 +/- 64 1650 +/- 13 1844 +/- 70 -0.882990613 0.049562082 0.590125477 0.2589213 SS18 synovial sarcoma translocation, chromosome 18 179 +/- 34 427 +/- 43 209 +/- 34 419 +/- 49 0.00124615 0.00896963 0.00299713 0.062158137 SSU72 SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae) 2015 +/- 93 2625 +/- 115 1716 +/- 93 2050 +/- 54 0.002266345 0.015168408 0.111942881 0.067295625 ST3GAL6 ST3 beta-galactoside alpha-2,3-sialyltransferase 6 690 +/- 106 1161 +/- 162 768 +/- 42 966 +/- 63 0.039073465 0.033661876 0.012021888 0.074533991 ST6GALNACST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferas 172 +/- 52 131 +/- 25 89 +/- 27 181 +/- 22 -0.494846146 0.027555818 0.708649901 0.196984254 ST6GALNACST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferas 776 +/- 23 926 +/- 60 825 +/- 32 928 +/- 25 0.055348109 0.031676665 0.006031079 0.345575065 STAT2 signal transducer and activator of transcription 2, 113kDa 863 +/- 108 1063 +/- 113 815 +/- 45 976 +/- 46 0.229758225 0.03469534 0.10122636 0.069811195 STOM stomatin 2053 +/- 253 2990 +/- 261 1953 +/- 163 2755 +/- 171 0.027515679 0.008200839 0.063237653 0.157507683 STRC stereocilin 345 +/- 54 551 +/- 31 332 +/- 31 472 +/- 34 0.010851421 0.015353896 0.054073404 0.074431642 SUPT4H1 suppressor of Ty 4 homolog 1 (S. cerevisiae) 854 +/- 45 1062 +/- 48 905 +/- 30 1088 +/- 32 0.0099546 0.00239974 0.053306637 0.113993594 TAS2R14 Taste receptor, type 2, member 14 101 +/- 20 203 +/- 19 104 +/- 21 230 +/- 27 0.004438424 0.005973521 0.036893291 0.098243184 TATDN3 TatD DNase domain containing 3 116 +/- 9 169 +/- 11 138 +/- 16 192 +/- 10 0.0041436 0.02164698 0.07241459 0.055340933 TBL1X transducin (beta)-like 1X-linked 155 +/- 28 369 +/- 21 212 +/- 17 525 +/- 99 0.000145665 0.033161203 0.003346196 0.073986264

S4- MSI correlated and non-correlated ARGs.xls Page 38 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG TCEAL4 transcription elongation factor A (SII)-like 4 8509 +/- 229 10384 +/- 384 8133 +/- 200 10329 +/- 553 0.00289964 0.013325761 0.097909518 0.138275737 TIMM17A translocase of inner mitochondrial membrane 17 homolog A (yeast) 4599 +/- 193 5229 +/- 399 4545 +/- 37 5734 +/- 354 0.196432216 0.027968519 0.885345521 0.246688963 TJP1 tight junction protein 1 (zona occludens 1) 881 +/- 94 1108 +/- 148 851 +/- 67 1056 +/- 37 0.229593724 0.029728453 0.165711408 0.115754631 TJP2 tight junction protein 2 (zona occludens 2) 2291 +/- 204 3125 +/- 237 2144 +/- 139 2859 +/- 124 0.024080242 0.004053353 0.021048548 0.066068672 TMBIM1 transmembrane BAX inhibitor motif containing 1 2080 +/- 216 2514 +/- 203 1362 +/- 54 1608 +/- 56 0.173426436 0.012063122 0.09321828 0.137968757 TMCO3 transmembrane and coiled-coil domains 3 771 +/- 94 966 +/- 81 781 +/- 44 952 +/- 37 0.146913058 0.015859224 0.070880413 0.16682445 TMEM106C transmembrane protein 106C 1970 +/- 98 2345 +/- 128 1940 +/- 209 2804 +/- 219 0.043413259 0.019261834 0.312849989 0.22733026 TMEM11 transmembrane protein 11 845 +/- 33 981 +/- 25 769 +/- 31 945 +/- 37 0.00892987 0.006195045 0.139897829 0.106812496 TMEM116 transmembrane protein 116 119 +/- 13 198 +/- 25 90 +/- 10 142 +/- 15 0.022720757 0.023265683 0.00913731 0.065412938 TMEM125 transmembrane protein 125 1877 +/- 348 2703 +/- 395 920 +/- 69 1320 +/- 139 0.148153575 0.042879253 0.03925191 0.098260234 TMEM42 transmembrane protein 42 1365 +/- 45 1466 +/- 33 1244 +/- 38 1458 +/- 62 0.101339459 0.022843879 0.372759651 0.186549401 TMEM48 transmembrane protein 48 109 +/- 18 137 +/- 12 135 +/- 6 164 +/- 4 0.232457296 0.003927966 0.391118219 0.109833919 TMTC4 transmembrane and tetratricopeptide repeat containing 4 1389 +/- 212 2022 +/- 276 751 +/- 81 984 +/- 63 0.100947558 0.049565076 0.115955074 0.183471703 TNFAIP8L1 tumor necrosis factor, alpha-induced protein 8-like 1 625 +/- 37 647 +/- 29 658 +/- 40 775 +/- 14 0.647160379 0.032228654 0.88913924 0.075513875 TNS3 tensin 3 5926 +/- 520 7061 +/- 361 4964 +/- 174 6047 +/- 403 0.106794346 0.052473325 0.393751323 0.350209298 TOM1L1 Target of myb1-like 1 (chicken) 114 +/- 13 121 +/- 6 130 +/- 12 171 +/- 11 0.631857575 0.030308589 0.626439221 0.095746474 TPP1 tripeptidyl peptidase I 1777 +/- 184 2252 +/- 226 1825 +/- 88 2210 +/- 122 0.135099084 0.035489624 0.109958197 0.072627957 TREX2 three prime repair exonuclease 2 95 +/- 10 117 +/- 10 117 +/- 14 160 +/- 11 0.152964498 0.039351889 0.60879764 0.277286121 TRIM37 tripartite motif-containing 37 6886 +/- 322 6344 +/- 360 6293 +/- 202 6895 +/- 149 -0.28801761 0.041014136 0.09343696 0.124105633 TRUB2 TruB pseudouridine (psi) synthase homolog 2 (E. coli) 855 +/- 93 1100 +/- 56 795 +/- 42 1011 +/- 37 0.052142304 0.003952869 0.128420178 0.100419906 TXNL1 thioredoxin-like 1 2413 +/- 328 2952 +/- 186 3020 +/- 129 3692 +/- 218 0.190997911 0.034489609 0.296000373 0.059513589 TYROBP TYRO protein tyrosine kinase binding protein 1055 +/- 57 1424 +/- 146 717 +/- 24 899 +/- 63 0.053300789 0.041325655 0.006013855 0.149642205 UBAP2L ubiquitin associated protein 2-like 1045 +/- 48 1221 +/- 34 950 +/- 19 1068 +/- 43 0.014530624 0.047736246 0.03302701 0.201222102 UBE2H ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) 3587 +/- 143 4508 +/- 50 3408 +/- 125 4376 +/- 229 0.000799338 0.0091898 0.001633609 0.083514757 UNC13C unc-13 homolog C (C. elegans) 555 +/- 100 536 +/- 112 1134 +/- 113 1508 +/- 113 -0.904621618 0.04444122 0.94897662 0.153211586 UQCRC1 ubiquinol-cytochrome c reductase core protein I 5480 +/- 38 5588 +/- 258 5191 +/- 86 5734 +/- 178 0.695534957 0.034439338 0.473698116 0.123746442 USP31 ubiquitin specific peptidase 31 360 +/- 19 436 +/- 27 499 +/- 36 687 +/- 45 0.045849929 0.011486388 0.133505683 0.121915743 XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku a 1545 +/- 90 1864 +/- 59 1424 +/- 33 1774 +/- 97 0.016491456 0.01936134 0.248647388 0.118649987 YIF1A Yip1 interacting factor homolog A (S. cerevisiae) 1268 +/- 101 1457 +/- 104 804 +/- 64 1043 +/- 52 0.220786051 0.01763122 0.130302185 0.29882994 YTHDC1 YTH domain containing 1 3734 +/- 134 4179 +/- 118 3676 +/- 128 4478 +/- 290 0.031495913 0.048116818 0.003623977 0.154046625 ZF HCF-binding transcription factor Zhangfei 481 +/- 22 590 +/- 26 608 +/- 20 692 +/- 20 0.0098783 0.016804892 0.007960292 0.350000711 ZNF131 Zinc finger protein 131 (clone pHZ-10) 124 +/- 18 238 +/- 29 160 +/- 22 267 +/- 25 0.010052177 0.011395349 0.126156615 0.066808291 ZNF134 zinc finger protein 134 (clone pHZ-15) 473 +/- 12 593 +/- 38 437 +/- 27 544 +/- 37 0.023399139 0.048350318 0.008654068 0.303351624 ZNF25 zinc finger protein 25 1239 +/- 41 1519 +/- 51 1305 +/- 50 1653 +/- 73 0.001854661 0.005249539 0.085885628 0.065991508 ZNF336 zinc finger protein 336 1688 +/- 83 1861 +/- 80 1511 +/- 33 1629 +/- 15 0.165353585 0.013896344 0.090900778 0.109354234 ZNF45 zinc finger protein 45 279 +/- 18 336 +/- 17 332 +/- 22 400 +/- 18 0.049501338 0.045353643 0.241044901 0.225942957 ZNF669 zinc finger protein 669 417 +/- 25 647 +/- 62 504 +/- 23 634 +/- 44 0.011643971 0.038528957 0.017252972 0.059934437 ZNFN1A1 zinc finger protein, subfamily 1A, 1 (Ikaros) 114 +/- 15 175 +/- 22 102 +/- 11 150 +/- 6 0.044945208 0.005622566 0.00837135 0.080888941 ZNRF2 zinc and ring finger 2 122 +/- 11 116 +/- 9 87 +/- 7 125 +/- 7 -0.667415035 0.004701507 0.726488896 0.078907218 ZRANB1 zinc finger, RAN-binding domain containing 1 849 +/- 44 1040 +/- 45 887 +/- 57 1073 +/- 52 0.012514974 0.039302018 0.25207767 0.26501462 Downregulated with Age in DG, not MSI Correlated ABTB2 Ankyrin repeat and BTB (POZ) domain containing 2 654 +/- 122 485 +/- 39 505 +/- 32 342 +/- 18 -0.236530963 -0.002265181 0.502144408 0.083242602 ACIN1 apoptotic chromatin condensation inducer 1 1859 +/- 171 1790 +/- 102 2092 +/- 94 1822 +/- 45 -0.735092343 -0.035290304 0.856289181 0.099341331 ACOT9 acyl-CoA thioesterase 9 370 +/- 33 344 +/- 40 284 +/- 9 218 +/- 20 -0.638310345 -0.02498055 0.671546112 0.283966613

S4- MSI correlated and non-correlated ARGs.xls Page 39 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG ACVR1B activin A receptor, type IB 1767 +/- 140 1532 +/- 52 1675 +/- 66 1338 +/- 98 -0.163381207 -0.023760859 0.201727547 0.249095982 ACY1L2 aminoacylase 1-like 2 224 +/- 39 230 +/- 24 450 +/- 41 321 +/- 40 0.895181939 -0.050237417 0.593761763 0.320936358 ANKRD43 ankyrin repeat domain 43 1626 +/- 115 1329 +/- 135 1930 +/- 112 1590 +/- 92 -0.125068566 -0.044002582 0.120734501 0.146407266 ANKRD52 ankyrin repeat domain 52 1020 +/- 66 863 +/- 45 1073 +/- 53 917 +/- 18 -0.080713305 -0.03065156 0.143621937 0.172988569 ARL6IP ADP-ribosylation factor-like 6 interacting protein 153 +/- 21 148 +/- 10 183 +/- 10 128 +/- 9 -0.810586051 -0.002747514 0.834831774 0.123177274 ARMCX1 armadillo repeat containing, X-linked 1 1267 +/- 41 1222 +/- 26 1385 +/- 38 1242 +/- 47 -0.378400402 -0.043795629 0.506433042 0.36170325 ASPHD2 aspartate beta-hydroxylase domain containing 2 919 +/- 80 730 +/- 79 1141 +/- 46 992 +/- 32 -0.122917394 -0.027085321 0.06845479 0.055795056 ATP6V1C1 ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1 998 +/- 141 706 +/- 35 1092 +/- 143 659 +/- 56 -0.094191529 -0.027764951 0.167291058 0.16054081 ATP8B2 ATPase, Class I, type 8B, member 2 1260 +/- 36 1102 +/- 43 1508 +/- 74 1279 +/- 39 -0.018717994 -0.027391031 0.071600827 0.129279316 BCL9L B-cell CLL/lymphoma 9-like 590 +/- 55 534 +/- 38 851 +/- 35 651 +/- 50 -0.417434605 -0.012773731 0.39314015 0.087479157 BICC1 Bicaudal C homolog 1 (Drosophila) 484 +/- 64 390 +/- 42 763 +/- 49 466 +/- 75 -0.249578651 -0.012458425 0.469360249 0.113669725 BRD2 bromodomain containing 2 683 +/- 52 469 +/- 53 945 +/- 41 572 +/- 72 -0.016524852 -0.003473977 0.280047378 0.097054419 BTBD12 BTB (POZ) domain containing 12 437 +/- 23 371 +/- 26 440 +/- 20 356 +/- 19 -0.083174481 -0.013971534 0.13391893 0.206370301 CA10 carbonic anhydrase X 1854 +/- 361 1852 +/- 301 4062 +/- 174 3455 +/- 172 -0.996745016 -0.03521384 0.956353675 0.106022879 CABP4 calcium binding protein 4 167 +/- 42 189 +/- 30 193 +/- 12 142 +/- 16 0.678144289 -0.035053297 0.3978992 0.097185871 CACNA1A calcium channel, voltage-dependent, P/Q type, alpha 1A subunit 961 +/- 89 664 +/- 44 1617 +/- 110 993 +/- 119 -0.018644654 -0.00419325 0.07476412 0.121622277 CAMSAP1 calmodulin regulated spectrin-associated protein 1 1864 +/- 55 1556 +/- 68 1653 +/- 70 1442 +/- 53 -0.005928848 -0.039506876 0.036371961 0.15892634 CAPZB capping protein (actin filament) muscle Z-line, beta 8247 +/- 582 6857 +/- 252 6783 +/- 248 5959 +/- 245 -0.065542162 -0.042500102 0.126065118 0.093828413 CARD9 caspase recruitment domain family, member 9 119 +/- 10 107 +/- 15 126 +/- 10 91 +/- 8 -0.509164665 -0.024558238 0.690461103 0.178182936 CARF collaborates/cooperates with ARF (alternate reading frame) protein 269 +/- 10 254 +/- 13 331 +/- 17 273 +/- 18 -0.405663578 -0.042435082 0.984876246 0.23283826 CCDC72 coiled-coil domain containing 72 4579 +/- 407 4039 +/- 229 3214 +/- 212 2593 +/- 139 -0.281026307 -0.038620586 0.504518198 0.07465981 CCDC84 coiled-coil domain containing 84 574 +/- 64 492 +/- 41 476 +/- 29 359 +/- 41 -0.311383712 -0.049202087 0.533931365 0.296487503 CDIPT CDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositol synthase) 3358 +/- 206 3149 +/- 168 3343 +/- 117 2839 +/- 95 -0.451126482 -0.008777598 0.366144649 0.08726787 CDK5R1 cyclin-dependent kinase 5, regulatory subunit 1 (p35) 3649 +/- 206 3034 +/- 85 4001 +/- 116 3504 +/- 169 -0.029701932 -0.04397449 0.015793702 0.307385394 CDKL1 Cyclin-dependent kinase-like 1 (CDC2-related kinase) 246 +/- 38 126 +/- 18 235 +/- 31 154 +/- 10 -0.024980063 -0.045552325 0.037404744 0.079403962 CETN3 centrin, EF-hand protein, 3 (CDC31 homolog, yeast) 1063 +/- 34 1071 +/- 46 1089 +/- 38 984 +/- 18 0.88956516 -0.040432614 0.2173904 0.226173754 CHCHD4 coiled-coil-helix-coiled-coil-helix domain containing 4 566 +/- 39 497 +/- 32 476 +/- 27 398 +/- 11 -0.201578789 -0.032703992 0.249583127 0.064587664 CHKA choline kinase alpha 607 +/- 41 586 +/- 44 764 +/- 36 646 +/- 32 -0.73894905 -0.035292452 0.861546537 0.056440529 CHSY1 carbohydrate (chondroitin) synthase 1 921 +/- 62 844 +/- 26 927 +/- 31 833 +/- 17 -0.289489252 -0.031811573 0.449344127 0.067020501 CIC capicua homolog (Drosophila) 2030 +/- 162 2055 +/- 125 3575 +/- 166 3066 +/- 138 0.9079011 -0.04287907 0.62724518 0.175126891 CKAP1 cytoskeleton associated protein 1 1486 +/- 142 1105 +/- 70 1406 +/- 119 962 +/- 130 -0.04564535 -0.033776237 0.283899216 0.20179708 CKMT1B creatine kinase, mitochondrial 1B 6947 +/- 213 5592 +/- 354 5522 +/- 246 4526 +/- 212 -0.010827979 -0.013361634 0.013891007 0.127060756 CLK4 CDC-like kinase 4 2680 +/- 52 2262 +/- 87 2336 +/- 119 1847 +/- 72 -0.003236721 -0.007870411 0.089082266 0.093570671 COL11A2 collagen, type XI, alpha 2 262 +/- 34 231 +/- 38 308 +/- 19 229 +/- 25 -0.556715608 -0.038333976 0.712347201 0.208044593 CREBBP CREB binding protein (Rubinstein-Taybi syndrome) 1537 +/- 76 1340 +/- 29 1670 +/- 25 1368 +/- 90 -0.047722227 -0.026138044 0.218216685 0.238085085 CTDSP2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2 2715 +/- 105 2657 +/- 150 2420 +/- 54 2153 +/- 92 -0.758013385 -0.042110021 0.711773627 0.147153688 CTTNBP2 cortactin binding protein 2 537 +/- 51 438 +/- 37 539 +/- 47 360 +/- 32 -0.154618487 -0.012403034 0.391687757 0.08725158 CYB561 cytochrome b-561 156 +/- 15 132 +/- 15 162 +/- 7 131 +/- 10 -0.284121849 -0.034607242 0.486114137 0.101723839 DCTN4 dynactin 4 (p62) 2167 +/- 114 1786 +/- 115 2057 +/- 72 1769 +/- 87 -0.03959171 -0.033853445 0.439314547 0.327635314 DEDD2 death effector domain containing 2 610 +/- 25 569 +/- 23 643 +/- 21 576 +/- 15 -0.256153101 -0.027965413 0.840206784 0.298835598 DENND1C DENN/MADD domain containing 1C 279 +/- 20 236 +/- 9 241 +/- 20 176 +/- 16 -0.089720524 -0.03421115 0.171088102 0.088209583 DFFA DNA fragmentation factor, 45kDa, alpha polypeptide 632 +/- 34 537 +/- 27 694 +/- 27 593 +/- 28 -0.054305353 -0.031207415 0.19627277 0.297678699 DKFZP779L1PRO0845 1349 +/- 59 1055 +/- 62 1202 +/- 31 955 +/- 46 -0.006254246 -0.002851053 0.117590109 0.084762499 DMTF1 cyclin D binding myb-like transcription factor 1 2324 +/- 160 2497 +/- 93 2237 +/- 55 2007 +/- 60 0.374362942 -0.021051196 0.143186402 0.153899292

S4- MSI correlated and non-correlated ARGs.xls Page 40 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG DPF1 D4, zinc and double PHD fingers family 1 1631 +/- 91 1083 +/- 88 1997 +/- 111 1529 +/- 115 -0.001498473 -0.017396385 0.00842478 0.107797752 DUSP14 dual specificity phosphatase 14 990 +/- 110 838 +/- 108 1439 +/- 43 1252 +/- 51 -0.347324404 -0.021930512 0.333594532 0.138896559 DYNC2LI1 dynein, cytoplasmic 2, light intermediate chain 1 663 +/- 56 564 +/- 36 662 +/- 33 520 +/- 17 -0.174754273 -0.005516776 0.336799351 0.072997445 DYRK2 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 831 +/- 140 590 +/- 62 593 +/- 37 489 +/- 19 -0.160623055 -0.040427269 0.126668945 0.133075353 DZIP1 DAZ interacting protein 1 1130 +/- 99 875 +/- 66 1406 +/- 101 904 +/- 129 -0.060363791 -0.015726199 0.107536641 0.067234275 ECHDC1 enoyl Coenzyme A hydratase domain containing 1 1796 +/- 60 1599 +/- 72 1651 +/- 62 1404 +/- 53 -0.061427477 -0.013713503 0.631625727 0.143303884 EEF1A2 eukaryotic translation elongation factor 1 alpha 2 12229 +/- 451 10754 +/- 445 11925 +/- 233 11098 +/- 267 -0.042273622 -0.046167272 0.014356403 0.381357782 EFHA2 EF-hand domain family, member A2 3623 +/- 194 3050 +/- 248 4234 +/- 119 3864 +/- 96 -0.100358469 -0.038747985 0.054441395 0.216094687 EIF5A2 eukaryotic translation initiation factor 5A2 834 +/- 58 677 +/- 46 773 +/- 40 626 +/- 24 -0.062434805 -0.013845144 0.252096345 0.088746517 ELF3 E74-like factor 3 (ets domain transcription factor, epithelial-specific ) 129 +/- 10 68 +/- 11 136 +/- 10 86 +/- 15 -0.002286897 -0.022561321 0.000951718 0.067420213 EPS15 epidermal growth factor receptor pathway substrate 15 6961 +/- 240 6152 +/- 333 6822 +/- 320 5815 +/- 233 -0.079764059 -0.03235132 0.517628101 0.151439674 ETFA electron-transfer-flavoprotein, alpha polypeptide (glutaric aciduria II) 763 +/- 80 635 +/- 93 926 +/- 45 580 +/- 121 -0.32330849 -0.043133771 0.653014952 0.190916327 FADS3 fatty acid desaturase 3 1186 +/- 74 995 +/- 86 1240 +/- 69 944 +/- 62 -0.123482717 -0.010979084 0.178920435 0.081198707 FAT4 FAT tumor suppressor homolog 4 (Drosophila) 293 +/- 14 306 +/- 33 708 +/- 23 485 +/- 48 0.734088954 -0.005929323 0.523440986 0.123458292 FBXL12 F-box and leucine-rich repeat protein 12 183 +/- 11 198 +/- 17 225 +/- 11 184 +/- 12 0.493511884 -0.04090149 0.225753291 0.178653124 FBXO22 F-box protein 22 180 +/- 15 116 +/- 11 204 +/- 17 146 +/- 19 -0.007921782 -0.047840719 0.043884135 0.074214805 FCHSD2 FCH and double SH3 domains 2 602 +/- 50 541 +/- 45 432 +/- 23 355 +/- 19 -0.386199664 -0.031366887 0.766445741 0.174599506 FCN3 ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen) 773 +/- 82 612 +/- 38 616 +/- 53 473 +/- 26 -0.119354515 -0.044076483 0.273237449 0.251666904 FKRP fukutin related protein 1114 +/- 40 971 +/- 30 1164 +/- 29 1050 +/- 21 -0.018582598 -0.010989279 0.037392418 0.111899405 FLJ30092 AF-1 specific protein phosphatase 3538 +/- 114 3147 +/- 187 3387 +/- 111 2870 +/- 54 -0.110710962 -0.003969496 0.051213415 0.077670869 FLJ37035 FLJ37035 protein 344 +/- 33 221 +/- 20 323 +/- 33 207 +/- 33 -0.012535482 -0.035517647 0.041448292 0.106054095 FLJ39237 FLJ39237 protein 202 +/- 20 175 +/- 23 202 +/- 16 152 +/- 11 -0.390375143 -0.028344303 0.853348508 0.350326323 FOXK1 forkhead box K1 1751 +/- 65 1597 +/- 115 1911 +/- 71 1533 +/- 131 -0.279784394 -0.042521364 0.387073506 0.094058559 FOXP1 forkhead box P1 285 +/- 29 214 +/- 23 213 +/- 24 144 +/- 16 -0.08604594 -0.043081771 0.74290022 0.22053733 FRMD3 FERM domain containing 3 159 +/- 13 124 +/- 13 144 +/- 8 115 +/- 10 -0.090892838 -0.048719648 0.107469981 0.057365271 FXYD7 FXYD domain containing ion transport regulator 7 3435 +/- 178 2940 +/- 109 4078 +/- 119 3624 +/- 147 -0.04407684 -0.042429162 0.00488822 0.483348228 G3BP Ras-GTPase-activating protein SH3-domain-binding protein 1810 +/- 99 1689 +/- 83 1957 +/- 54 1678 +/- 67 -0.37211519 -0.011907896 0.861534233 0.140315677 GABBR2 gamma-aminobutyric acid (GABA) B receptor, 2 1834 +/- 197 1551 +/- 193 2778 +/- 78 2396 +/- 114 -0.329709299 -0.026832838 0.29594896 0.324514968 GAPVD1 GTPase activating protein and VPS9 domains 1 1036 +/- 57 938 +/- 97 956 +/- 26 845 +/- 31 -0.405966411 -0.023562626 0.800810376 0.417411893 GARNL1 GTPase activating Rap/RanGAP domain-like 1 683 +/- 64 626 +/- 46 579 +/- 30 462 +/- 37 -0.485890005 -0.040098604 0.923399822 0.068734549 GCHFR GTP cyclohydrolase I feedback regulator 417 +/- 27 263 +/- 11 403 +/- 32 315 +/- 18 -0.00123335 -0.044121673 0.003602917 0.26266741 GEMIN6 gem (nuclear organelle) associated protein 6 120 +/- 23 102 +/- 10 117 +/- 13 77 +/- 3 -0.493262348 -0.024096823 0.256943141 0.077973918 GNL2 guanine nucleotide binding protein-like 2 (nucleolar) 347 +/- 23 320 +/- 17 374 +/- 23 301 +/- 18 -0.378445191 -0.03344959 0.835245674 0.109622239 GPR153 G protein-coupled receptor 153 395 +/- 32 371 +/- 45 374 +/- 8 300 +/- 22 -0.679134613 -0.024122979 0.921476421 0.14871703 GPR155 G protein-coupled receptor 155 3986 +/- 374 3280 +/- 279 5230 +/- 184 4681 +/- 151 -0.164042986 -0.046512652 0.333008264 0.250868344 GRM5 Glutamate receptor, metabotropic 5 4099 +/- 219 3135 +/- 171 3523 +/- 175 2644 +/- 98 -0.006510561 -0.002590439 0.006481496 0.085829727 GRTP1 Growth hormone regulated TBC protein 1 446 +/- 50 299 +/- 12 412 +/- 52 272 +/- 16 -0.030152815 -0.042958386 0.035210678 0.103772623 GTF2IRD2 GTF2I repeat domain containing 2 310 +/- 18 224 +/- 27 365 +/- 33 210 +/- 55 -0.028055011 -0.047209612 0.482695937 0.339189571 GTF3C4 General transcription factor IIIC, polypeptide 4, 90kDa 1590 +/- 79 1380 +/- 69 1484 +/- 45 1344 +/- 39 -0.072365264 -0.043751346 0.080517223 0.148008384 HADH2 hydroxyacyl-Coenzyme A dehydrogenase, type II 2567 +/- 64 2333 +/- 98 2257 +/- 48 2004 +/- 54 -0.078236206 -0.007018894 0.117523678 0.085956846 HECTD1 HECT domain containing 1 154 +/- 14 131 +/- 13 204 +/- 13 162 +/- 11 -0.238273049 -0.041531809 0.852478954 0.224897501 HERC1 hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 3490 +/- 87 3209 +/- 99 3290 +/- 56 3128 +/- 37 -0.05980791 -0.042149986 0.007128254 0.15256026 HERC4 hect domain and RLD 4 725 +/- 37 638 +/- 58 882 +/- 55 667 +/- 35 -0.240880598 -0.010633622 0.333892552 0.133846768 HIGD2A HIG1 domain family, member 2A 3641 +/- 182 2920 +/- 183 3456 +/- 163 2935 +/- 93 -0.019088664 -0.024690915 0.023971733 0.087432337

S4- MSI correlated and non-correlated ARGs.xls Page 41 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG HMGCR 3-hydroxy-3-methylglutaryl-Coenzyme A reductase 1890 +/- 172 1539 +/- 69 2130 +/- 95 1817 +/- 102 -0.101923573 -0.052680553 0.064745887 0.186102049 HNMT histamine N-methyltransferase 396 +/- 32 235 +/- 22 494 +/- 59 338 +/- 17 -0.002524332 -0.044935424 0.021349226 0.072377414 HP1BP3 heterochromatin protein 1, binding protein 3 5237 +/- 233 4163 +/- 95 5620 +/- 224 4368 +/- 255 -0.004208435 -0.005506694 0.030837236 0.073044113 HSP90B1 heat shock protein 90kDa beta (Grp94), member 1 5233 +/- 258 4774 +/- 119 5615 +/- 180 4876 +/- 111 -0.150045151 -0.008040943 0.453591825 0.200610975 HSPA12A heat shock 70kDa protein 12A 420 +/- 40 297 +/- 27 455 +/- 42 300 +/- 28 -0.030853522 -0.015229323 0.054125438 0.100142974 HSPC159 HSPC159 protein 145 +/- 22 117 +/- 17 161 +/- 15 118 +/- 10 -0.33280406 -0.04264681 0.211825933 0.056425333 IFIT5 interferon-induced protein with tetratricopeptide repeats 5 467 +/- 26 398 +/- 26 493 +/- 24 422 +/- 16 -0.091012978 -0.035868096 0.165892284 0.221069267 IKBKAP inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein 3512 +/- 159 3207 +/- 197 3071 +/- 108 2708 +/- 98 -0.257208496 -0.034273271 0.987911255 0.369116338 IPLA2(GAMMintracellular membrane-associated calcium-independent phospholipase A2 gamma 3034 +/- 116 2924 +/- 99 3184 +/- 174 2707 +/- 92 -0.488736044 -0.043351621 0.844156661 0.128193177 ITGA6 integrin, alpha 6 1277 +/- 56 1145 +/- 70 1213 +/- 98 804 +/- 50 -0.1737088 -0.006910234 0.617707414 0.075081462 ITGA8 integrin, alpha 8 101 +/- 17 101 +/- 14 126 +/- 12 92 +/- 3 -0.982265262 -0.035995202 0.915694298 0.177920495 ITGB1BP1 integrin beta 1 binding protein 1 832 +/- 41 713 +/- 53 853 +/- 30 743 +/- 28 -0.108726909 -0.025874579 0.098960687 0.139542575 KCMF1 potassium channel modulatory factor 1 904 +/- 45 798 +/- 43 861 +/- 25 761 +/- 23 -0.117733452 -0.01805466 0.118599745 0.186627563 KCNK17 potassium channel, subfamily K, member 17 175 +/- 17 111 +/- 10 253 +/- 20 177 +/- 20 -0.011001664 -0.023816161 0.072727066 0.084128799 KCTD3 potassium channel tetramerisation domain containing 3 1153 +/- 87 1018 +/- 49 1352 +/- 65 947 +/- 142 -0.213511732 -0.043040726 0.556885 0.316617013 KIF3B kinesin family member 3B 1592 +/- 199 1106 +/- 96 1784 +/- 164 1327 +/- 44 -0.062973647 -0.037930748 0.108525391 0.140866785 KLF13 Kruppel-like factor 13 1790 +/- 60 1158 +/- 151 2000 +/- 133 1313 +/- 196 -0.006850529 -0.022009748 0.148842536 0.386259152 KLHDC5 kelch domain containing 5 3067 +/- 144 2879 +/- 162 2960 +/- 80 2641 +/- 82 -0.405493792 -0.021610156 0.21106509 0.081382931 KLHL15 kelch-like 15 (Drosophila) 646 +/- 36 564 +/- 39 642 +/- 26 552 +/- 15 -0.154466202 -0.016238779 0.15678483 0.078429516 KLHL2 kelch-like 2, Mayven (Drosophila) 4816 +/- 510 3890 +/- 688 7319 +/- 565 5661 +/- 464 -0.306975408 -0.049756593 0.462715658 0.301801973 KRTCAP2 Keratinocyte associated protein 2 159 +/- 10 120 +/- 17 200 +/- 25 128 +/- 5 -0.081010084 -0.033836658 0.076362901 0.156934784 KTN1 kinectin 1 (kinesin receptor) 3032 +/- 249 3355 +/- 275 3498 +/- 199 2780 +/- 142 0.403113555 -0.017361537 0.127905871 0.264136844 LASS5 LAG1 longevity assurance homolog 5 (S. cerevisiae) 1328 +/- 43 993 +/- 51 1277 +/- 72 1037 +/- 33 -0.000565873 -0.01899967 0.000457208 0.127403657 LAT2 linker for activation of T cells family, member 2 246 +/- 22 214 +/- 23 266 +/- 22 203 +/- 17 -0.346152124 -0.047562637 0.606396122 0.104097832 LEREPO4 likely ortholog of mouse immediate early response, erythropoietin 4 3868 +/- 230 3508 +/- 145 4093 +/- 95 3354 +/- 211 -0.219624657 -0.020663146 0.280335556 0.126289426 LNX1 ligand of numb-protein X 1 2480 +/- 118 1905 +/- 184 2474 +/- 174 1901 +/- 108 -0.028569789 -0.022991433 0.002603438 0.156600864 LPHN1 latrophilin 1 3273 +/- 149 2840 +/- 165 3901 +/- 181 3300 +/- 124 -0.080278813 -0.023977973 0.084034496 0.281603487 LRP11 low density lipoprotein receptor-related protein 11 4775 +/- 167 4237 +/- 116 4754 +/- 143 4135 +/- 226 -0.026691143 -0.054358382 0.213604269 0.477677495 LRRC28 leucine rich repeat containing 28 389 +/- 32 357 +/- 24 478 +/- 25 395 +/- 14 -0.447188926 -0.021642949 0.307742226 0.141729817 LRRC3B leucine rich repeat containing 3B 1060 +/- 61 844 +/- 59 1402 +/- 91 1140 +/- 58 -0.029330681 -0.040665115 0.054211861 0.273995944 LRRN5 leucine rich repeat neuronal 5 1461 +/- 68 1120 +/- 25 1554 +/- 45 1289 +/- 57 -0.002903974 -0.006256578 0.007370126 0.064886225 LSM5 LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) 860 +/- 61 808 +/- 19 1126 +/- 50 958 +/- 52 -0.445189711 -0.045761881 0.544638411 0.088792508 LTBP1 latent transforming growth factor beta binding protein 1 361 +/- 17 236 +/- 20 447 +/- 38 218 +/- 28 -0.00071457 -0.000958711 0.098246847 0.0743978 LUZP2 Leucine zipper protein 2 148 +/- 21 101 +/- 16 116 +/- 17 69 +/- 8 -0.103660365 -0.035561964 0.077490758 0.065440402 LZIC Leucine zipper and CTNNBIP1 domain containing 187 +/- 11 146 +/- 17 188 +/- 10 125 +/- 20 -0.073598956 -0.033696692 0.666954141 0.150632468 MAD2L1BP MAD2L1 binding protein 801 +/- 48 804 +/- 14 744 +/- 14 700 +/- 10 0.952030525 -0.030986567 0.843314552 0.150394656 MAGEH1 melanoma antigen family H, 1 5195 +/- 193 4645 +/- 235 6391 +/- 306 5229 +/- 199 -0.101413156 -0.012417285 0.106845299 0.089632653 MAP1LC3A microtubule-associated protein 1 light chain 3 alpha 3967 +/- 274 2363 +/- 245 4800 +/- 253 2961 +/- 404 -0.00144192 -0.00636558 0.059834641 0.163614208 MARK2 MAP/microtubule affinity-regulating kinase 2 823 +/- 33 702 +/- 30 941 +/- 40 765 +/- 25 -0.023035541 -0.005706683 0.135183632 0.132393049 MAX MYC associated factor X 1031 +/- 87 779 +/- 39 1103 +/- 54 911 +/- 39 -0.034213781 -0.019564295 0.067448525 0.207610623 MCF2L MCF.2 cell line derived transforming sequence-like 169 +/- 11 113 +/- 10 232 +/- 24 135 +/- 8 -0.00333276 -0.007613212 0.033674994 0.067935138 MEST mesoderm specific transcript homolog (mouse) 2485 +/- 318 2364 +/- 191 3814 +/- 151 3349 +/- 120 -0.7521878 -0.039852063 0.860051759 0.079115205 MFAP1 microfibrillar-associated protein 1 1476 +/- 22 1315 +/- 35 1367 +/- 41 1252 +/- 27 -0.004145342 -0.047851898 0.002324551 0.062969353 MKKS McKusick-Kaufman syndrome 2005 +/- 127 1739 +/- 127 2049 +/- 26 1793 +/- 93 -0.169457199 -0.049556332 0.363338164 0.191451442

S4- MSI correlated and non-correlated ARGs.xls Page 42 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) 569 +/- 45 480 +/- 41 470 +/- 32 363 +/- 16 -0.174970977 -0.019892831 0.738367087 0.056107414 MOBKL2B MOB1, Mps One Binder kinase activator-like 2B (yeast) 138 +/- 10 100 +/- 11 127 +/- 7 86 +/- 4 -0.028521632 -0.001403645 0.062958159 0.055919928 MRPL22 mitochondrial ribosomal protein L22 706 +/- 20 652 +/- 26 641 +/- 17 556 +/- 21 -0.128264949 -0.014088609 0.242144385 0.2526271 MYO1B myosin IB 240 +/- 40 180 +/- 30 189 +/- 25 121 +/- 13 -0.250919236 -0.045612624 0.463544264 0.074196096 N4BP3 Nedd4 binding protein 3 109 +/- 7 101 +/- 7 119 +/- 9 90 +/- 4 -0.40977475 -0.022975305 0.513906381 0.138844257 NAP5 Nck-associated protein 5 211 +/- 31 212 +/- 27 162 +/- 11 123 +/- 8 0.973619971 -0.02114515 0.659389709 0.111594389 NDUFV1 NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa 2923 +/- 155 2506 +/- 143 2595 +/- 65 2288 +/- 108 -0.076226055 -0.045770623 0.127150538 0.255318262 NFIL3 nuclear factor, interleukin 3 regulated 495 +/- 40 462 +/- 38 822 +/- 51 669 +/- 29 -0.562878274 -0.032291699 0.563072425 0.125402367 NKD2 naked cuticle homolog 2 (Drosophila) 229 +/- 12 225 +/- 18 335 +/- 22 274 +/- 16 -0.873328408 -0.053988924 0.951125038 0.189399495 NOG Noggin 599 +/- 81 458 +/- 67 733 +/- 50 542 +/- 33 -0.208994192 -0.011914076 0.177280619 0.099486263 NOTUM notum pectinacetylesterase homolog (Drosophila) 87 +/- 16 107 +/- 11 136 +/- 15 92 +/- 6 0.321693695 -0.036054903 0.331144337 0.101913829 NPEPL1 aminopeptidase-like 1 143 +/- 12 156 +/- 13 174 +/- 8 136 +/- 11 0.481346845 -0.025600501 0.799900684 0.118329193 NPLOC4 nuclear protein localization 4 homolog (S. cerevisiae) 1165 +/- 45 1126 +/- 45 1102 +/- 47 968 +/- 34 -0.557939536 -0.046812294 0.62373956 0.143394738 NRXN1 neurexin 1 10862 +/- 245 9499 +/- 402 10764 +/- 142 9886 +/- 287 -0.019307751 -0.033930344 0.031491478 0.148727641 NUBP1 nucleotide binding protein 1 (MinD homolog, E. coli) 679 +/- 25 548 +/- 12 607 +/- 21 506 +/- 19 -0.002200702 -0.006861505 0.025427377 0.077320833 NUFIP2 nuclear fragile X mental retardation protein interacting protein 2 761 +/- 34 710 +/- 36 845 +/- 39 746 +/- 16 -0.328267414 -0.051373998 0.748716035 0.058155841 OCRL oculocerebrorenal syndrome of Lowe 5167 +/- 351 2651 +/- 414 4835 +/- 441 3145 +/- 483 -0.000997411 -0.030467437 0.028945575 0.277285789 OSBPL6 oxysterol binding protein-like 6 2412 +/- 155 1905 +/- 45 2468 +/- 135 1963 +/- 75 -0.02064243 -0.012145114 0.093437471 0.227481628 P2RX5 purinergic receptor P2X, ligand-gated ion channel, 5 1338 +/- 74 1083 +/- 93 1528 +/- 87 1238 +/- 63 -0.058172178 -0.02551087 0.097410602 0.069564403 PABPC5 poly(A) binding protein, cytoplasmic 5 153 +/- 5 103 +/- 10 160 +/- 15 110 +/- 8 -0.003193097 -0.019984793 0.089753987 0.125170727 PAK3 P21 (CDKN1A)-activated kinase 3 227 +/- 18 167 +/- 19 271 +/- 16 203 +/- 14 -0.048201664 -0.010497467 0.050619504 0.077254286 PAN3 PAN3 polyA specific ribonuclease subunit homolog (S. cerevisiae) 1456 +/- 117 1346 +/- 43 1368 +/- 61 1193 +/- 38 -0.411628265 -0.040479339 0.669218585 0.173963616 PASK PAS domain containing serine/threonine kinase 171 +/- 15 144 +/- 18 171 +/- 11 130 +/- 11 -0.279468739 -0.029535745 0.317124345 0.223027582 PCDH10 protocadherin 10 956 +/- 148 1051 +/- 135 653 +/- 82 380 +/- 31 0.647461568 -0.019472837 0.718784454 0.070046703 PCSK2 proprotein convertase subtilisin/kexin type 2 3146 +/- 220 2328 +/- 198 2601 +/- 119 2049 +/- 105 -0.020417862 -0.006951289 0.01232449 0.060491027 PCTK2 PCTAIRE protein kinase 2 4630 +/- 476 3780 +/- 295 5133 +/- 337 4095 +/- 92 -0.166030517 -0.026239816 0.202842072 0.134702193 PDZRN4 PDZ domain containing RING finger 4 961 +/- 82 934 +/- 58 831 +/- 30 732 +/- 22 -0.790399473 -0.028344943 0.525706147 0.095878713 PEX14 peroxisomal biogenesis factor 14 1046 +/- 74 925 +/- 41 1172 +/- 79 945 +/- 26 -0.190371854 -0.034154868 0.084989484 0.06614945 PFDN1 prefoldin subunit 1 3757 +/- 69 3495 +/- 74 3517 +/- 72 3281 +/- 42 -0.026757747 -0.0223749 0.003461971 0.103413488 PIK3R2 phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta) 5364 +/- 460 3833 +/- 348 4458 +/- 93 3892 +/- 156 -0.025607679 -0.017781087 0.021090149 0.095284858 PINK1 PTEN induced putative kinase 1 5970 +/- 290 3813 +/- 313 5732 +/- 418 4288 +/- 150 -0.000503668 -0.016543541 0.005077902 0.11399275 PKIA Protein kinase (cAMP-dependent, catalytic) inhibitor alpha 252 +/- 9 223 +/- 13 277 +/- 14 229 +/- 14 -0.09414404 -0.041147802 0.527385335 0.171524677 PLAC9 placenta-specific 9 1835 +/- 282 1130 +/- 254 1524 +/- 212 916 +/- 172 -0.093037075 -0.053552202 0.432079614 0.313665902 PLEK pleckstrin 301 +/- 28 251 +/- 18 267 +/- 22 206 +/- 13 -0.167953082 -0.044631566 0.373950719 0.134891651 PLEKHG6 pleckstrin homology domain containing, family G (with RhoGef domain) member 6 368 +/- 21 360 +/- 55 442 +/- 17 355 +/- 17 -0.889861604 -0.006192481 0.80103137 0.248088221 PLXNB3 plexin B3 457 +/- 56 453 +/- 43 384 +/- 19 316 +/- 15 -0.953366316 -0.01945359 0.654622675 0.075026734 PNLDC1 poly(A)-specific ribonuclease (PARN)-like domain containing 1 203 +/- 62 42 +/- 1 212 +/- 57 60 +/- 6 -0.047861142 -0.044611804 0.195803754 0.13121375 PPM1E protein phosphatase 1E (PP2C domain containing) 2356 +/- 357 1782 +/- 252 2989 +/- 122 2473 +/- 54 -0.221718191 -0.006500382 0.271745248 0.074406024 PRKRIR protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) 1194 +/- 105 1357 +/- 44 1231 +/- 45 1098 +/- 38 0.196904013 -0.049312552 0.262210648 0.112415974 PRO0149 PRO0149 protein 3917 +/- 388 3006 +/- 191 4784 +/- 216 3768 +/- 160 -0.071797767 -0.004642731 0.149122069 0.069218685 PROCA1 proline-rich cyclin A1-interacting protein 188 +/- 17 132 +/- 6 195 +/- 11 162 +/- 7 -0.01904541 -0.03118106 0.104932479 0.258450261 PRPS2 phosphoribosyl pyrophosphate synthetase 2 1966 +/- 106 1443 +/- 29 1604 +/- 58 1369 +/- 46 -0.003525035 -0.011978454 0.001513869 0.084104019 PRSS22 protease, serine, 22 376 +/- 33 245 +/- 15 364 +/- 29 215 +/- 21 -0.009083568 -0.002358389 0.046989389 0.059613239 PSPC1 paraspeckle component 1 4137 +/- 535 3110 +/- 267 3353 +/- 193 2357 +/- 289 -0.127704105 -0.023390631 0.511080638 0.158301759

S4- MSI correlated and non-correlated ARGs.xls Page 43 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG PTBP2 polypyrimidine tract binding protein 2 2307 +/- 103 1782 +/- 161 2298 +/- 39 1811 +/- 152 -0.02381853 -0.030830317 0.438613881 0.216102612 PUM2 pumilio homolog 2 (Drosophila) 2633 +/- 77 1935 +/- 111 2753 +/- 131 1997 +/- 141 -0.000604458 -0.003648263 0.05562315 0.081645211 QSCN6L1 quiescin Q6-like 1 715 +/- 62 579 +/- 58 1381 +/- 87 1148 +/- 33 -0.143148012 -0.044211937 0.17520605 0.149820638 RAB35 RAB35, member RAS oncogene family 778 +/- 29 811 +/- 19 718 +/- 14 670 +/- 5 0.367490517 -0.015565703 0.590646323 0.088326224 REV3L REV3-like, catalytic subunit of DNA polymerase zeta (yeast) 1626 +/- 79 1510 +/- 68 1694 +/- 74 1485 +/- 50 -0.293951404 -0.046104157 0.287801253 0.098756475 RHOBTB2 Rho-related BTB domain containing 2 686 +/- 32 533 +/- 29 663 +/- 45 502 +/- 41 -0.005244694 -0.028313851 0.007948868 0.08870149 RIT2 Ras-like without CAAX 2 609 +/- 31 437 +/- 55 775 +/- 62 568 +/- 48 -0.025575445 -0.027875631 0.077862913 0.062894532 RNF10 ring finger protein 10 3235 +/- 192 2478 +/- 121 3155 +/- 83 2677 +/- 130 -0.009517874 -0.017449864 0.082708791 0.409322341 RNF149 ring finger protein 149 862 +/- 59 752 +/- 33 938 +/- 25 772 +/- 46 -0.140754258 -0.018644142 0.263253546 0.060960675 RNF165 ring finger protein 165 582 +/- 25 444 +/- 19 525 +/- 34 419 +/- 29 -0.001550293 -0.042494447 0.043446064 0.191880592 RNF40 ring finger protein 40 697 +/- 31 616 +/- 10 876 +/- 27 750 +/- 31 -0.043586854 -0.014864958 0.340172388 0.108042495 RPIB9 Rap2-binding protein 9 1388 +/- 111 1270 +/- 94 1665 +/- 42 1512 +/- 53 -0.437013689 -0.054717856 0.453265894 0.351287158 RPL31 ribosomal protein L31 232 +/- 31 172 +/- 17 275 +/- 9 198 +/- 25 -0.128384949 -0.03293557 0.336434313 0.26945184 RPL37 ribosomal protein L37 1311 +/- 97 1831 +/- 155 1601 +/- 107 1229 +/- 57 0.020800471 -0.016960897 0.031384133 0.072104543 RPS12 ribosomal protein S12 4329 +/- 211 3150 +/- 239 3422 +/- 186 2508 +/- 191 -0.004218543 -0.00763633 0.093213947 0.150396701 RRAGC Ras-related GTP binding C 2912 +/- 161 3207 +/- 157 2579 +/- 91 2330 +/- 56 0.220753668 -0.047747997 0.430171906 0.0631591 RSBN1 round spermatid basic protein 1 1261 +/- 90 1171 +/- 26 1341 +/- 54 1091 +/- 43 -0.37371077 -0.005686273 0.609460738 0.058652398 RUNX1T1 runt-related transcription factor 1; translocated to, 1 (cyclin D-related) 2489 +/- 125 1740 +/- 89 2274 +/- 171 1707 +/- 184 -0.000846719 -0.051017315 0.01645597 0.495650684 SCGB3A1 secretoglobin, family 3A, member 1 110 +/- 8 98 +/- 11 132 +/- 9 101 +/- 7 -0.404156732 -0.023415436 0.916556785 0.092843164 SDK2 sidekick homolog 2 (chicken) 931 +/- 129 836 +/- 94 667 +/- 46 527 +/- 27 -0.563398746 -0.030214457 0.519932468 0.091562392 SEC14L1 SEC14-like 1 (S. cerevisiae) 4771 +/- 153 4085 +/- 221 5018 +/- 284 3990 +/- 299 -0.031449238 -0.03470786 0.459700401 0.261595307 SEMA6B sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B 2141 +/- 281 1472 +/- 204 2572 +/- 104 2191 +/- 53 -0.08595555 -0.013093356 0.105883426 0.195480987 SETBP1 SET binding protein 1 177 +/- 22 138 +/- 17 245 +/- 24 131 +/- 20 -0.19183427 -0.005449354 0.448002102 0.13556878 SETD6 SET domain containing 6 357 +/- 14 262 +/- 22 362 +/- 8 285 +/- 23 -0.006155276 -0.024760878 0.071339234 0.157550143 SFXN1 sideroflexin 1 947 +/- 84 649 +/- 46 800 +/- 33 630 +/- 29 -0.014753663 -0.004024948 0.022844764 0.055998664 SFXN3 sideroflexin 3 918 +/- 58 841 +/- 34 1028 +/- 50 787 +/- 44 -0.290513104 -0.00549148 0.547595196 0.069852941 SGPL1 sphingosine-1-phosphate lyase 1 613 +/- 28 522 +/- 26 640 +/- 24 524 +/- 12 -0.040205869 -0.002866218 0.231950816 0.057885563 SGSH N-sulfoglucosamine sulfohydrolase (sulfamidase) 274 +/- 62 276 +/- 24 224 +/- 8 190 +/- 9 0.975656175 -0.023478068 0.867132759 0.188117293 SH3MD4 SH3 multiple domains 4 1732 +/- 142 1583 +/- 171 1400 +/- 71 1205 +/- 43 -0.518297455 -0.047786914 0.928266656 0.259189821 SHANK2 SH3 and multiple ankyrin repeat domains 2 3124 +/- 137 2183 +/- 63 2942 +/- 130 2120 +/- 154 -0.000417935 -0.003276605 0.000954574 0.074945144 SLC12A5 solute carrier family 12, (potassium-chloride transporter) member 5 9260 +/- 395 7923 +/- 381 8510 +/- 287 7677 +/- 214 -0.035165521 -0.045788367 0.015477601 0.202189808 SLC25A25 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25 1121 +/- 61 974 +/- 52 1300 +/- 67 1101 +/- 43 -0.096748348 -0.03672137 0.03067862 0.114505719 SLC2A6 solute carrier family 2 (facilitated glucose transporter), member 6 542 +/- 34 512 +/- 27 621 +/- 36 490 +/- 11 -0.497871229 -0.013617841 0.527077813 0.10169118 SLC35E3 solute carrier family 35, member E3 812 +/- 93 775 +/- 32 861 +/- 58 712 +/- 17 -0.724466004 -0.049851375 0.943595422 0.079531213 SLC5A3 solute carrier family 5 (inositol transporters), member 3 289 +/- 39 240 +/- 38 429 +/- 33 342 +/- 17 -0.391168577 -0.052975677 0.388174594 0.078915707 SLFNL1 schlafen-like 1 165 +/- 16 140 +/- 13 183 +/- 10 119 +/- 9 -0.234170457 -0.001115942 0.140924896 0.06609075 SMS spermine synthase 1440 +/- 104 1361 +/- 60 1756 +/- 49 1533 +/- 42 -0.531937147 -0.007359754 0.606413307 0.117973188 SMU1 Smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) 1021 +/- 44 845 +/- 49 1000 +/- 21 892 +/- 31 -0.024100861 -0.021557898 0.061954055 0.167536525 SORBS2 Sorbin and SH3 domain containing 2 432 +/- 19 353 +/- 14 405 +/- 14 294 +/- 41 -0.007594933 -0.049321265 0.078440767 0.364208937 SOX11 SRY (sex determining region Y)-box 11 664 +/- 52 459 +/- 46 303 +/- 32 221 +/- 13 -0.014598477 -0.04875412 0.011762268 0.055596693 SPG20 spastic paraplegia 20, spartin (Troyer syndrome) 1329 +/- 71 1486 +/- 143 1459 +/- 35 1302 +/- 45 0.3571879 -0.025409897 0.022835492 0.19617511 SRrp35 serine-arginine repressor protein (35 kDa) 851 +/- 61 650 +/- 57 860 +/- 61 692 +/- 42 -0.036873369 -0.05114665 0.063738218 0.19318906 SSB Sjogren syndrome antigen B (autoantigen La) 2114 +/- 118 1777 +/- 99 2359 +/- 70 1882 +/- 138 -0.054697646 -0.021627807 0.061261408 0.162029076 SSTR2 somatostatin receptor 2 181 +/- 26 94 +/- 7 135 +/- 18 66 +/- 7 -0.018011504 -0.010196622 0.005480342 0.055425313

S4- MSI correlated and non-correlated ARGs.xls Page 44 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG STRBP spermatid perinuclear RNA binding protein 926 +/- 84 754 +/- 29 785 +/- 30 663 +/- 42 -0.098304001 -0.049348173 0.212619169 0.250843153 SUB1 SUB1 homolog (S. cerevisiae) 198 +/- 12 153 +/- 13 233 +/- 7 187 +/- 11 -0.029380853 -0.009334405 0.070572835 0.200849812 SULT1A3 sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3 375 +/- 64 226 +/- 35 292 +/- 26 202 +/- 21 -0.078387951 -0.024139531 0.243997888 0.154238119 SUOX sulfite oxidase 266 +/- 15 222 +/- 19 301 +/- 8 220 +/- 19 -0.100405305 -0.008878695 0.261111961 0.096741605 SURF4 surfeit 4 844 +/- 29 730 +/- 19 751 +/- 33 661 +/- 21 -0.010483129 -0.048622922 0.102185092 0.242072965 SYNGR4 synaptogyrin 4 345 +/- 36 312 +/- 23 289 +/- 21 221 +/- 22 -0.459799236 -0.054898421 0.697862786 0.260900479 SYT11 synaptotagmin XI 2979 +/- 924 523 +/- 91 2747 +/- 804 738 +/- 96 -0.044900169 -0.05440499 0.045545221 0.091856384 SYT17 synaptotagmin XVII 1654 +/- 367 1225 +/- 137 1568 +/- 101 1227 +/- 51 -0.314314277 -0.018829893 0.265448908 0.055700564 SYTL2 synaptotagmin-like 2 300 +/- 78 429 +/- 112 148 +/- 20 94 +/- 10 0.368996022 -0.048362188 0.437356576 0.156373851 TAF5L TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa 511 +/- 14 496 +/- 10 521 +/- 15 459 +/- 15 -0.402735909 -0.015641475 0.628870365 0.189536634 TBC1D14 TBC1 domain family, member 14 2125 +/- 84 1815 +/- 92 1795 +/- 52 1639 +/- 43 -0.031696434 -0.047244724 0.222267241 0.2088684 TCERG1 transcription elongation regulator 1 1003 +/- 111 1042 +/- 31 1253 +/- 60 1001 +/- 75 0.745116253 -0.029946796 0.597618483 0.293000746 TDRD6 tudor domain containing 6 160 +/- 22 164 +/- 14 169 +/- 12 130 +/- 7 0.880838832 -0.026081677 0.724895544 0.064398813 TFG TRK-fused gene 5280 +/- 199 4443 +/- 99 4815 +/- 94 4186 +/- 147 -0.006471633 -0.008782778 0.008840003 0.243183596 TGFB3 transforming growth factor, beta 3 505 +/- 61 393 +/- 48 651 +/- 37 486 +/- 46 -0.182678045 -0.022850667 0.548310955 0.084469964 THBS3 thrombospondin 3 546 +/- 53 445 +/- 55 780 +/- 44 597 +/- 55 -0.214843713 -0.031589826 0.281920388 0.068747408 THRAP1 thyroid hormone receptor associated protein 1 2516 +/- 97 2385 +/- 106 2836 +/- 108 2482 +/- 58 -0.383922275 -0.021616483 0.918259791 0.067799701 TIMM10 translocase of inner mitochondrial membrane 10 homolog (yeast) 1629 +/- 120 1047 +/- 77 1208 +/- 47 967 +/- 68 -0.003153302 -0.021238258 0.00548846 0.144075924 TM2D1 TM2 domain containing 1 743 +/- 38 713 +/- 50 856 +/- 54 690 +/- 41 -0.642776696 -0.037847121 0.714512496 0.179244168 TM9SF2 transmembrane 9 superfamily member 2 1860 +/- 104 971 +/- 362 1861 +/- 69 746 +/- 349 -0.057368775 -0.031588019 0.441130942 0.162169021 TMEM126B transmembrane protein 126B 118 +/- 16 90 +/- 10 126 +/- 9 89 +/- 10 -0.171889319 -0.02630581 0.27253049 0.057572169 TMEM132B transmembrane protein 132B 1274 +/- 123 930 +/- 71 1051 +/- 67 730 +/- 54 -0.041704541 -0.004741841 0.240402673 0.156696458 TMEM132E transmembrane protein 132E 138 +/- 10 118 +/- 18 232 +/- 19 159 +/- 19 -0.347427978 -0.023538523 0.121017297 0.070941713 TMEM25 transmembrane protein 25 2517 +/- 184 2187 +/- 216 3846 +/- 199 3254 +/- 106 -0.271729579 -0.032155932 0.272918691 0.129767471 TMEM93 transmembrane protein 93 2312 +/- 100 1990 +/- 126 2258 +/- 82 1965 +/- 87 -0.075387554 -0.03794458 0.186627666 0.060756516 TMEPAI transmembrane, prostate androgen induced RNA 899 +/- 154 699 +/- 73 772 +/- 55 601 +/- 44 -0.277781776 -0.038059058 0.169874262 0.160455495 TNRC4 trinucleotide repeat containing 4 4534 +/- 210 3677 +/- 260 5558 +/- 185 4760 +/- 252 -0.029148199 -0.035166821 0.010782622 0.125680243 TNRC9 trinucleotide repeat containing 9 230 +/- 31 204 +/- 19 217 +/- 25 147 +/- 15 -0.488732146 -0.042513666 0.476932917 0.099416737 TP53BP2 tumor protein p53 binding protein, 2 3969 +/- 331 4293 +/- 320 4425 +/- 200 3879 +/- 120 0.496882968 -0.047499175 0.249564584 0.298378966 TRIM46 tripartite motif-containing 46 886 +/- 40 715 +/- 65 1011 +/- 67 787 +/- 42 -0.052703686 -0.02173346 0.018977454 0.072136194 TRPM7 transient receptor potential cation channel, subfamily M, member 7 356 +/- 40 334 +/- 21 378 +/- 30 285 +/- 20 -0.629170302 -0.031434627 0.967614532 0.203034517 TSGA2 testis specific A2 homolog (mouse) 658 +/- 150 274 +/- 41 370 +/- 40 239 +/- 30 -0.049773829 -0.027797187 0.044111728 0.113750324 TSPAN3 tetraspanin 3 4001 +/- 86 3629 +/- 136 3966 +/- 66 3699 +/- 96 -0.047447907 -0.053879539 0.0498443 0.24013768 TSPYL3 TSPY-like 3 (pseudogene) 716 +/- 53 638 +/- 63 867 +/- 80 645 +/- 47 -0.361300679 -0.044290446 0.432536145 0.14258546 TTC19 tetratricopeptide repeat domain 19 2924 +/- 101 1559 +/- 359 3189 +/- 138 1757 +/- 528 -0.011318191 -0.051446777 0.065680723 0.168026491 TUBB2B tubulin, beta 2B 3672 +/- 237 3414 +/- 308 3355 +/- 175 2679 +/- 191 -0.521948217 -0.029003671 0.745515808 0.091447835 TUFM Tu translation elongation factor, mitochondrial 1075 +/- 75 895 +/- 60 995 +/- 31 811 +/- 30 -0.091891493 -0.002053773 0.079960181 0.055395999 TUSC1 tumor suppressor candidate 1 965 +/- 92 888 +/- 78 1306 +/- 67 1105 +/- 34 -0.537008828 -0.031015491 0.469194186 0.092669085 TUSC3 tumor suppressor candidate 3 593 +/- 43 437 +/- 30 743 +/- 35 545 +/- 36 -0.015208368 -0.003574256 0.026811227 0.078995526 TXNDC10 thioredoxin domain containing 10 1152 +/- 79 1252 +/- 69 1427 +/- 33 1248 +/- 63 0.36633805 -0.046451728 0.677995212 0.184753814 TXNDC4 thioredoxin domain containing 4 (endoplasmic reticulum) 1246 +/- 67 1050 +/- 62 1153 +/- 88 920 +/- 29 -0.057808702 -0.044765299 0.389629165 0.261277625 UBC ubiquitin C 6672 +/- 739 5772 +/- 426 7116 +/- 734 5191 +/- 278 -0.321669308 -0.04724725 0.412415109 0.182116236 UBE2V2 ubiquitin-conjugating enzyme E2 variant 2 1294 +/- 48 1322 +/- 90 1405 +/- 72 1126 +/- 68 0.789299928 -0.020027247 0.917609583 0.120357726 UBE3A ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) 6737 +/- 339 5705 +/- 371 5837 +/- 154 5224 +/- 81 -0.067393974 -0.008728416 0.169678269 0.065819319

S4- MSI correlated and non-correlated ARGs.xls Page 45 Young vs Aged MSI Correlations Symbol Description Young CA Aged CA Young DG Aged DG in CA in DG in CA in DG UCN urocortin 399 +/- 28 387 +/- 22 380 +/- 17 317 +/- 13 -0.741043517 -0.017920635 0.98870892 0.101513143 UNC45A unc-45 homolog A (C. elegans) 863 +/- 83 692 +/- 25 923 +/- 40 731 +/- 59 -0.096748095 -0.029505868 0.36848176 0.427112546 USP15 ubiquitin specific peptidase 15 2158 +/- 53 1639 +/- 107 2009 +/- 51 1697 +/- 92 -0.003134811 -0.023252573 0.058322123 0.324435591 USP4 ubiquitin specific peptidase 4 (proto-oncogene) 2228 +/- 74 1806 +/- 34 2258 +/- 99 1973 +/- 65 -0.001323917 -0.04187396 0.015351275 0.35433655 WDFY1 WD repeat and FYVE domain containing 1 1082 +/- 175 808 +/- 190 857 +/- 69 579 +/- 68 -0.316251788 -0.018631604 0.815625579 0.218160488 WDR47 WD repeat domain 47 4038 +/- 157 3797 +/- 170 4306 +/- 111 3939 +/- 111 -0.322562237 -0.045221443 0.143074397 0.172406686 WDR74 WD repeat domain 74 744 +/- 14 687 +/- 26 664 +/- 37 566 +/- 24 -0.095725318 -0.053059872 0.201972744 0.104317297 WHDC1 WAS protein homology region 2 domain containing 1 276 +/- 18 251 +/- 22 322 +/- 19 263 +/- 13 -0.402533246 -0.033975828 0.238003182 0.061157987 XPNPEP1 X-prolyl aminopeptidase (aminopeptidase P) 1, soluble 1226 +/- 73 1063 +/- 32 1269 +/- 56 1109 +/- 24 -0.080738107 -0.035698474 0.032885789 0.076763929 YTHDF2 YTH domain family, member 2 2059 +/- 97 1665 +/- 58 2492 +/- 66 2178 +/- 98 -0.007866082 -0.031414013 0.112830832 0.432848963 YTHDF3 YTH domain family, member 3 3848 +/- 269 3599 +/- 115 3721 +/- 144 3177 +/- 92 -0.424114136 -0.012372504 0.629213173 0.062283594 ZBTB24 zinc finger and BTB domain containing 24 626 +/- 63 454 +/- 23 608 +/- 38 435 +/- 38 -0.039583377 -0.01011329 0.187721069 0.150985666 ZBTB7C zinc finger and BTB domain containing 7C 448 +/- 21 348 +/- 43 483 +/- 41 358 +/- 31 -0.074595508 -0.037871787 0.080420567 0.157762147 ZMPSTE24 zinc metallopeptidase (STE24 homolog, yeast) 681 +/- 23 630 +/- 24 787 +/- 36 633 +/- 19 -0.153944684 -0.005706849 0.389119114 0.108993539 ZNF161 zinc finger protein 161 1955 +/- 205 2188 +/- 214 1741 +/- 43 1557 +/- 39 0.451333515 -0.011179921 0.120171025 0.072079243 ZNF44 zinc finger protein 44 227 +/- 19 193 +/- 5 249 +/- 13 199 +/- 12 -0.132475522 -0.022050181 0.447714259 0.200024235 ZNF452 zinc finger protein 452 148 +/- 21 151 +/- 15 209 +/- 9 177 +/- 7 0.896417921 -0.02352519 0.942629341 0.076394206 ZNF469 zinc finger protein 469 394 +/- 35 350 +/- 17 320 +/- 12 250 +/- 18 -0.294885976 -0.015176462 0.276885464 0.190211263 ZNF573 zinc finger protein 573 187 +/- 34 144 +/- 13 251 +/- 30 152 +/- 28 -0.2795361 -0.039264785 0.562013145 0.431785401 ZNF598 zinc finger protein 598 783 +/- 25 699 +/- 38 826 +/- 24 723 +/- 33 -0.098271107 -0.03515865 0.023228201 0.071239091 ZNF615 zinc finger protein 615 148 +/- 12 112 +/- 12 154 +/- 12 102 +/- 14 -0.05701512 -0.019855503 0.204070629 0.278452395 ZNF650 zinc finger protein 650 1129 +/- 62 1186 +/- 66 1399 +/- 98 1075 +/- 82 0.536553057 -0.031841049 0.061982236 0.201040628 ZNF658 zinc finger protein 658 201 +/- 19 171 +/- 8 278 +/- 14 206 +/- 22 -0.20671084 -0.02606887 0.258691386 0.125236317 ZNF664 zinc finger protein 664 4855 +/- 461 4156 +/- 264 5217 +/- 185 4351 +/- 285 -0.224378196 -0.037811086 0.462258175 0.189498969 ZNF74 zinc finger protein 74 147 +/- 15 91 +/- 13 170 +/- 12 113 +/- 14 -0.018936105 -0.016370007 0.112743819 0.206491307 ZNRD1 zinc ribbon domain containing 1 926 +/- 41 625 +/- 37 853 +/- 26 720 +/- 30 -0.000268689 -0.009907701 0.05962573 0.099213787 ZWINT ZW10 interactor 592 +/- 49 356 +/- 55 462 +/- 44 332 +/- 21 -0.009324758 -0.032175899 0.007406112 0.088879118 ZXDB zinc finger, X-linked, duplicated B 667 +/- 61 496 +/- 39 665 +/- 46 479 +/- 61 -0.044558801 -0.041047339 0.260164736 0.248674131 ZZEF1 zinc finger, ZZ-type with EF-hand domain 1 1717 +/- 133 1604 +/- 63 1605 +/- 32 1429 +/- 41 -0.465890658 -0.009886225 0.65265442 0.078559695

S4- MSI correlated and non-correlated ARGs.xls Page 46