P53 and Ceramide As Collaborators in the Stress Response

Int. J. Mol. Sci. 2013, 14, 4982-5012; doi:10.3390/ijms14034982 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Review p53 and Ceramide as Collaborators in the Stress Response Rouba Hage-Sleiman 1,2,*, Maria O. Esmerian 1,2, Hadile Kobeissy 2 and Ghassan Dbaibo 1,2 1 Department of Pediatrics and Adolescent Medicine, Division of Pediatric Infectious Diseases, Faculty of Medicine, American University of Beirut, P.O. Box 11-0236 Riad El Solh, 1107 2020 Beirut, Lebanon; E-Mails: [email protected] (M.O.E.); [email protected] (G.D.) 2 Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, P.O. Box 11-0236 Riad El Solh, 1107 2020 Beirut, Lebanon; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +961-1-350-000 (ext. 4883). Received: 26 December 2012; in revised form: 22 January 2013 / Accepted: 1 February 2013 / Published: 1 March 2013 Abstract: The sphingolipid ceramide mediates various cellular processes in response to several extracellular stimuli. Some genotoxic stresses are able to induce p53-dependent ceramide accumulation leading to cell death. However, in other cases, in the absence of the tumor suppressor protein p53, apoptosis proceeds partly due to the activity of this “tumor suppressor lipid”, ceramide. In the current review, we describe ceramide and its roles in signaling pathways such as cell cycle arrest, hypoxia, hyperoxia, cell death, and cancer. In a specific manner, we are elaborating on the role of ceramide in mitochondrial apoptotic cell death signaling. Furthermore, after highlighting the role and mechanism of action of p53 in apoptosis, we review the association of ceramide and p53 with respect to apoptosis. Strikingly, the hypothesis for a direct interaction between ceramide and p53 is less favored. Recent data suggest that ceramide can act either upstream or downstream of p53 protein through posttranscriptional regulation or through many potential mediators, respectively. Keywords: ceramide; p53; apoptosis; sphingolipids; mitochondria; signaling; Bcl2 family; caspase Int. J. Mol. Sci. 2013, 14 4983 1. Introduction Ceramide is a key sphingolipid that acts as a second messenger for multiple extracellular stimuli to mediate many cellular processes. Ceramide signaling, conserved throughout evolution, was found to be involved in death signaling in many systems. Since yeast cells undergo a cell death mechanism that resembles apoptosis, the sphingomyelin pathway appears evolutionarily older than the caspase-mediated death programs described in higher organisms [1]. Most DNA damaging agents and genotoxic stressors induce apoptosis in p53-dependent pathways. However, in the absence of p53, programmed cell death proceeds and is partly mediated by the “tumor suppressor lipid”, ceramide. Nevertheless, many stimuli can cause p53-dependent ceramide accumulation leading to cell death. In this review, we intend to focus on the role of ceramide in signaling pathways of apoptosis and try to shed light on its relation with p53. 2. Ceramide Biosynthesis Ceramide is an N-acylsphingosine consisting of a fatty acid bound to the amino group of the sphingoid base, sphingosine. In general, ceramides are usually found with mono-unsaturated or saturated fatty acids of various lengths that significantly alter their physical properties. Many natural ceramides are being isolated and might be of therapeutic importance such as cameroonemide A from the plant Helichrysum cameroonense [2] and ceramide/cerebroside from the stem bark of Ficus mucuso [3]. Ceramides with 16–24 carbon fatty acyl chains are the most commonly found in mammalian cellular membranes. Depending on the cell type and stimulus, ceramide is generated by three major pathways (Figure 1). First, in the cell membrane, sphingomyelin can be broken down to ceramide in a reaction catalyzed by sphingomyelinases (neutral, acidic, or alkaline). Second, the de novo synthesis of ceramide occurs by the condensation of palmitate and serine to form 3-keto-dihydrosphingosine that is further reduced to dihydrosphingosine. This pathway, generating ceramide from less complex molecules, is catalyzed by the enzyme serine palmitoyl transferase (SPT) and occurs in the endoplasmic reticulum (ER). Dihydrosphingosine is then acylated by the enzyme (dihydro) ceramide synthase (CerS) of which there are 6 isoforms (CerS1-6) to produce dihydroceramide [4]. In its turn, dihydroceramide is then converted to ceramide by the dihydroceramide desaturase enzyme and transported to the Golgi by either vesicular trafficking or by the ceramide transfer protein CERT [5]. Endoplasmic reticulum–trans-Golgi membrane contacts are required for nonvesicular ceramide transport. These contact sites facilitate the transfer of newly synthesized ceramide from ER to sphingomyelin synthase (SMS) located at the trans-Golgi via CERT [5]. The third pathway is termed the salvage pathway. It contributes from 50% to 90% of sphingolipid biosynthesis, and occurs through the breakdown of complex sphingolipids and glycosphingolipids in acidic cellular compartments such as the late endosomes and lysosomes, to produce sphingosine. For instance, sphingomyelin can be converted to ceramide by acid sphingomyelinase, encoded by a distinct gene than that of neutral sphingomyelinase [6]. Furthermore, ceramide can be hydrolyzed by acid ceramidase to form sphingosine and a free fatty acid, both of which, and unlike ceramide, are able to leave the lysosome. Ceramide synthase family members probably trap free sphingosine released from the lysosome at the surface of the endoplasmic reticulum or in its associated membranes [3,4]. Int. J. Mol. Sci. 2013, 14 4984 Figure 1. Metabolic pathways of ceramide synthesis and degradation: Names of organelles (A to D) are underlined. Names of enzymes are written in italic. Black solid arrows are used to show metabolic conversions. Blue dashed arrows indicate protein-mediated transfers. Abbreviations: SPT: Serine Palmitoyltransferase; CerS: Ceramide synthase; CERT: ceramide transfer protein. SMS: sphingomyelin synthase; A-SMase: Acid Sphingomyelinase, N-SMase: Neutral sphingomyelinase; GCS: Glucosylceramide synthase. C. Golgi SM SMS SM Ceramide N-SMase GCS D. Lysosome CERT GlcCer SM B. Endoplasmic Reticulum Dihydroceramide Ceramide A-SMase Desaturase CerS Ceramide Dihydrosphingosine Ceramidase SPT Serine + Palmitoyl-CoA Sphingosine Sphingosine Ceramidase SMS Ceramide SM A. Plasma Membrane N-SMase Additional studies revealed that variation in free Mg2+ causes sustained changes in membrane phospholipids and second messengers resulting in the activation of intracellular signal transcription molecules such as NF-κB, proto-oncogenes c-fos and c-jun, MAPK and MAPKK in vascular smooth muscle cells in vitro [7]. More importantly, variations in Mg2+ cause truncation of membrane fatty acids, significant activation of sphingomyelinase (SMase) and alterations in membrane sphingomyelin leading to the release of ceramides. Consequently, because of all these modifications, apoptotic caspases become activated and mitochondrial cytochrome c is released [8–10]. Furthermore, and contrary to sphingomyelinase, SMS directly regulates cellular ceramide and diacylglycerol (DAG) levels [11]. It was recently shown that Mg2+ deficiency upregulates SMS and p53 in diverse cardiovascular tissues and cells. Mg2+-deficient environments drive the de novo synthesis of ceramide via the activation of three enzymes in the sphingolipid pathway: SPT, SMS, and CerS. The lower the Mg2+ is, the greater is the synthesis of ceramide [12]. Although the cytoplasmic generated ceramide was described to play important roles in mediating signaling pathways, membrane ceramide share equivalent importance in mediating cellular pathways and functional processes. For instance, ceramide generated at the exoplasmic leaflet of the plasma membrane self-associates and mediates the formation of ceramide-rich platforms (CRPs) with diameters of 200 nm up to several microns. These macrodomains are thought to derive from Int. J. Mol. Sci. 2013, 14 4985 sphingolipid and cholesterol-enriched rafts and seem to be active sites for protein oligomerization during transmembrane signaling [13]. However, some exceptions exist where membrane ceramide does not participate in signaling. For instance, in the breast cancer cell line MCF7, ceramide generation at the outer leaflet of the plasma membrane following the exogenous addition of bacterial sphingomyelinase does not induce cell death [14–16]. 3. Ceramide and Cellular Signaling Ceramide accumulates under specific conditions to play an important role in signaling pathways. Indeed, ceramide is a topological cell-signaling lipid that forms functionally distinct endomembrane structures and vesicles termed “sphingosome” that organize into a specialized apical compartment in polarized cells [17]. In general, growth factors, chemical agents, and environmental stresses generate ceramide in order to mediate proliferation, membrane receptor functions, immune inflammatory responses, differentiation, cell adhesion, growth arrest, or apoptosis [6,12,18–20]. Furthermore, there is evidence that ceramide mediates another terminal cellular event, senescence [21]. Indeed, ceramide contributes to senescence by activating the growth suppressor pathway through retinoblastoma (Rb) dephosphorylation and the mitogenic pathway mediated by c-Fos and AP-1 [22]. Moreover, ceramide can regulate other cellular

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