Phylogenetic Position of the Phacotaceae Within the Chlamydophyceae As Revealed by Analysis of 18S Rdna and Rbcl Sequences
J Mol Evol (1998) 47:420–430 © Springer-Verlag New York Inc. 1998 Phylogenetic Position of the Phacotaceae Within the Chlamydophyceae as Revealed by Analysis of 18S rDNA and rbcL Sequences D. Hepperle,1 H. Nozaki,2 S. Hohenberger,3 V.A.R. Huss,3 E. Morita,2 L. Krienitz1 1 Institute of Freshwater Ecology & Inland Fisheries, Department of Limnology of Stratified Lakes, Alte Fischerhu¨tte 2, D-16775 Neuglobsow, Germany 2 University of Tokyo, Department of Biological Sciences, Graduate School of Science, Hongo, Bunkyo, Tokyo 113, Japan 3 University of Erlangen, Institute of Botany and Pharmaceutical Biology, Staudtstr. 5, D-91058 Erlangen, Germany Received: 9 June 1997 / Accepted: 17 October 1997 Abstract. Four genera of the Phacotaceae (Phacotus, and ഛ86.6% in the rbcL gene. It showed major similari- Pteromonas, Wislouchiella, Dysmorphococcus), a family ties to the 18S rDNA of Dunaliella salina, with 95.3%, of loricated green algal flagellates within the Volvocales, and to the rbcL sequence of Chlamydomonas tetragama, were investigated by means of transmission electron mi- with 90.3% sequence homology. Additionally, the croscopy and analysis of the nuclear encoded small- Phacotaceae sensu stricto exclusively shared 10 (rbcL: 4) subunit ribosomal RNA (18S rRNA) genes and the plas- characters which were present neither in other Chlam- tid-encoded rbcL genes. Additionally, the 18S rDNA of ydomonadales nor in Dysmorphococcus globosus. Dif- Haematococcus pluvialis and the rbcL sequences of ferent phylogenetic analysis methods confirmed the hy- Chlorogonium elongatum, C. euchlorum, Dunaliella pothesis that the Phacotaceae are polyphyletic. The parva, Chloromonas serbinowii, Chlamydomonas ra- Phacotaceae sensu stricto form a stable cluster with af- diata, and C.
[Show full text]