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International Conference “Sericulture Challenges in the 21st Century” (Serichal 2007) & the 3rd BACSA meeting, 18 -21 September 2007, Vratza, Bulgaria Supported by the FAO Regular Programme P R O C E E D I N G S Black, Caspian Seas and Central Asia Silk Association (BACSA) www.bacsa-silk.org Sericulture Experiment Station, Vratza, Bulgaria Vratza 2007 2 International Conference “Sericulture Challenges in the 21st Century” (Serichal 2007) & the 3rd BACSA meeting, 18 -21 September 2007, Vratza, Bulgaria Supported by the FAO Regular Programme P R O C E E D I N G S Black, Caspian Seas and Central Asia Silk Association (BACSA) www.bacsa-silk.org Sericulture Experiment Station, Vratza, Bulgaria Vratza 2007 3 TABLE OF CONTENTS Conference programme………………………...…………………………..……………………………..7 Opening Speech Dr P. Tzenov………..………………………...……………………………………………………………10 Present status and utilization of sericulture germplasm and comparative studies of different silkworm hybrids performance for sericultural enterprise development in the Black, Caspian seas and Central Asia (BACSA) region” P.Tzenov.......................................................................................................................................................13 Section 1. Moriculture and non-mulberry food plants for sericigenous insects: selection, propagation and cultivation Assessment of harvestable leaf yield and effect of defoliation on seed yield and qualityin castor (Ricinus communis), the primary food plant of Eri silkworm, Samia Cynthia ricini boisduval T. Philip, M.A.Shekhar, M. Rekha, B.K. Kariappa………….………………………………………...…..36 Mulberry’s fruit treatment properties and Its many-sided apply N.Sichinava, E.Kalatozishvili, A.Giorgadze, L.A.Mujiri…………..…………………………………..…41 Mulberry cultivation through organic farming in India – an upcoming concept Geetha N. Murthy……..………………………………………………………………………………...…44 Researches concerning “in vitro” cloning of some parental mulberry varieties used to obtain hybrid lineas of morus genus D. Brailoiu Tanase, G. Negru, A. Rosu, S. Danaila-Guidea........................................................................55 Different kind of mulberry’s seed’s economical fitness define to get rootstock N. B. Sichinava, E. Kimucadze, M. Bokuchava, N. Nacvlishvili……………………….………………...62 Mulberry phytoplasmal disease mey be transmissed by soil fungy N. Chkhaidze……………………………………………………………………………………...…….…63 Effect of bio-stimulator ASMA # 1 on mulberry leaf curl N.Baramidze, Z.Putkaradze.........................................................................................................................64 Utilization of salt affected soils through reclamation and growing alkali tolerant genotypes K. Sathyanarayana, T. Mogili, M. Munirathnam Reddy, A. Sarkar…………...……………...…………. 65 Studies on the status of biochemical constituents in four morphotypes of muga food plant som (Persea bombycina) A.B.Baruah and P.K.Baruah ………………………………………………………………………………72 Prognostics of economic indices of mulberry by correlated selection indices T.Revazishvili & T.Dalalishvili………………………...…………………………..……………………..75 Fruit capacity and leaf intensity of new mulberry breeds in feeding of mulberry silkworm of upper ages Z. Chitadze………………………………………….………………………….………………………….76 History of mulberry selection and perspectives in Georgia T. Dalalishvili, B. Sakandelidze, T. Revazishvili, Z. Chitadze………..………..…………………………77 Evaluation of som (persea bombycina) germplasm accessions trough bioassay for rearing performance and biochemical analysis of leaves K. Neog, R. Chakravorty, S.N. Gogoi…………………………….……………………….………………81 Effect of micro nutrients on seed cocoon floss production R. N. Dutta, T. Jayappa, K. L. Rajanna and S .S. Sindagi………………………………………...………88 Studies on leaf productivity improvement in mulberry through cost effective technologies in 21st century B. N. Susheelamma, S. B. Dandin, M. Rekha……………………………………………………….……89 Technology for preliminary selection of mulberry genotypes at nursery stage R.K Fotadar, A. Dhar, M. A.Khan……………………………………………………………………. ….94 Biochemical methods of testing of economic-useful attributes forming productivity and nutritiousness of mulberry Homidy Kh.S………………………………………………………………………………...………….…95 Morphological characteristics of the leaves from local Bulgarian mulberry varieties Z. Petkov……………………………………………………………………………………...……….….105 Morphological characteristics of the shoots and buds from local Bulgarian mulberry varieties Z. Petkov………………………………………………………………………………...…….……….…110 4 Efficacy of different plant extracts as antifeedant against mulberry mealy bug Maconellicoccus hirsutus M. Maheswari, H. M. Revanasiddadiah, G. Govindaiah………………………..……….………………116 Section 2. Silkworm Bombyx mori L. 2.1.Silkworm rearing and feeding Result of influence the biostimulator - Biorag on quantity of Albumens in a mulberry’s leaf and mulberry silkworm’s hemolymphe E. Kimutsadze, Z. Futkaradze, R. Gakhokidze, S. MaMulia…………………………………....………123 Comparison of haemolymph protein profiles between multivoltine and bivoltine silkworm breeds under temperature stress S. Sreekumar, S. Chitra, S.K. Ashwath, G.K.Rajesh…………………..……………………………….125 Result of using the biostimulator – Bioragi in sericulture in Georgia E. Kimutsadze, Z. Futkaradze, G. Kimutsadze, N. Natsvlishvili………………………...……..……….134 2.2. Silkworm genetics and breeding The silkworm race “J90” A. Matei………………………………………………………………….……………….………………137 Estimation of the selection material on interrelation of the attributes forming productivity and nutritiousness of the mulberry leaf with activity of enzymes Homidy Kh.S………………………………………………………………………………..…………...142 Genetic resources of the silkworm in Ukraine. A level of scrutiny and prospects of use Yu. V. Lyyashenko, О. V. Galanova…….……………………………………………………..….…….149 Theoretical and practical bases of adaptive selection of the silkworm in Azerbaijan Abbasov B.H…………………………………………………….………….………………..……….….150 Results of Mulberry Silkworm Clone Selection Ts. Tabliashvili, L. Tabliashviili, Z. Tskaruashvili, I. Chargeishvili……….…………………..…….….159 Evaluation of new-created sex-limited silkworm Bombyx mori L. lines N. Petkov, P. Tzenov, Z. Petkov and I. Vassileva………………………..…………….……………...…162 Mathematical forecasting of selection effect in silkworm Bombyx mori L. on the basis of shell ratio character N. Petkov, P. Tzenov, Z. Petkov and I. Vassileva…………………………………………...……..…….169 The performances of the hybrids resulted from dialele crossings between the lines selected on the basis of general combinative ability G. Dinita, A. Matei, D. S. Dezmirean.......................................................................................................173 Highly productive mulberry silkworm breeds and hybrids for industrial feeding A.Dzneladze, I.Maisuradze, M.Prangishvili, A. Matiashvili, L.Latsabidze, Z.Tskaruashvili…………..181 Perspective breeds of mulberry silkworm with the view of cocoon filament length and thinness A. Giorgadze, A. Dzneladze, L. Kobakhidze, M. Svanidze, T.Revazishvili, I.Chargeishvili…………...182 Mulberry silkworm perspective breeds with very long and fine cocoon thread A. Dzneladze, N. Stepanishvili, A. Giorgadze, L. Kobakhidze, Z. Tskaruashvili, M.Svanidze, E.Tsotskolauri, I.Chargeishvili………..………………………………………………..…183 2.3. Silkworm egg production Effect of short-term preservation of multivoltine eggs (Nistari strain) at low temperatures on hatching S. Cappellozza, L. Cappellozza, A. Mastella, A. Saviane, E. Cosi, P.F. Roversi…….……………...…..189 Occurrence of Unfertilized Eggs in the Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) T. Singh, N.M. Biram Saheb, B. Saratchandra…………………………….…………………………..…194 Effect of varied low temperature preservation of male moths on reproductive parameters in the silkworm Bombyx mori L. S. T. Christiana, T.M. Veeraiah………………………………………...………………….………..……195 Tasar Silkworm Seed Production in Private Sector under Special SGSY Projects in Bihar and Jharkhand - A Breakthrough S. Amarnath, K. Sathyanarayana and R.P. Khanna…………………………………...…………...….….204 Standardization of seed processing and seed supply for optimization of resource utilization for Oak Tasar development in sub Himalayan region 5 R.N.Dutta, S.S.Sindagi, B.M.K.Singh, S.K.Sinha, S. Rai, Y.V. Ramanjaneyulu…...…...……………....212 Results of Biostimulator Effect on Mulberry Silkworm Grain N. Baramidze, M. Khutsishvili, Sh. Kharatishvili, I. Gujabidze, M. Tsikhiseli………..………………...213 2.4. Silkworm pathology Efficient preparations to control infections and mikrosporidiosis in the silkworm O. V. Galanova, I. O. Kirichenko, I.P.Sukhanova, O.V. Dmytriyeva……...……………………...….….217 Bioecological approaches to pest management in sericulture R. N. Singh, B. Saratchandra…………………………………………………………………..................218 Scope of machine learning techniques for disease forecasting in sericulture K. M. Rao, H. S. Phaniraj, S.Amarnath……………………………………………………………...…...228 Microbial infection on fifth instar larvae of tasar silkworm, Antheraea mylitta eco-race Bhandara D.D. Barsagade, M.N. Kadwey, M. M. Shinkhede …………………………………..…………..……..233 Section 3. Silkworms as biological models Immunity promotion and proteomic identification in mice upon exposure to manganese superoxide dismutase expressed in silkworm larvae,Bombyx mori L. Y. Miao, W. Yue, J. Liu, G. Li, X. Li, X. Wu…………………………………………….……………...234 Introduction of Gu-Bm-EK1 cell line from embryonic tissue of silkworm,Bombyx mori L. Matindoost, J. J. Sendi, H. Soleiman-Jahi, K. Etebari, F. Rahbarizadeh………………….…………..242 Studies concerning the utilization of Morus alba root