Molecular Genetics Lab @ SHSU Madhusudan Choudhary, Ph. D Lin Lin Anne Peters Graduate Student Graduate Student Cell Cycle Regulation Principal Investigator Gene Duplication Analysis My research interest involves the role of CtrA, My graduate research focuses on the gene a master transcription regulator, in the cell duplication analysis involving structural cycle regulation. The expression level of ctrA and functional constraints on duplicate gene during the different periods of cell cycle, genes. This study aims to understand the such as B (G1), C (S phase), and D (G2), role of gene duplication in evolution of controls the expression of a number of new metabolic functions as well as the structural and regulatory genes which set the origin of biodiversity of bacterial species. transition of the cell from one stage to another The project employed new computer soft during the cell cycle. wares as well as existing bioinformatics approaches. Anish Bavishi Undergraduate at Rice University Current Research Projects Kristen Schroeder Bacterial Genome Evolution Gene Duplication Analysis Undergraduate Student The dogma of bacterial genome structure, Cell cycle regulation in R. sphaeroides My research interests are to analyze gene consisting a single circular chromosome, has been duplication with respect to its level of challenged and the existence of multiple Genetic and evolutionary analyses of duplicate genes microarray expression level of the R. chromosomes in bacteria is no longer an exception. sphaeroides cells grown under different My research interest involves the origin of multiple Analysis of chromosomal origin of replication conditions. This functional analysis will chromosomes in prokaryotes and its connection in be further used to disrupt the duplicate the evolution complex genome development.  genes in order to understand the Whole genome comparison of bacterial species essentiality of the each of the duplicate gene for the survival of cells. Origin and evolution of mitochondria Randi Harbour Undergraduate Student Kristina Hernandez Analysis of Chromosomal Origin of Replication Recent Publications Recent Presentations Undergraduate Student We have employed two approaches to identify the Chromosomal Origins of Replication in origin of replication in chromosome I and II. R. sphaeroides  Schroeder, K., L. Lin, A. Bavishi, A. Peters, and M. Choudhary (2009) Abundance  Tim Johnson, Randi Harbour, Kristina Hernandez, Lin Lin, and Madhusudan Following the silico-based approach, 13 sequences After the initial identification of the of gene duplication and the evolution of specific metabolic functions in the complex Choudhary (2009) Identification of the chromosomal origins of replication (OriRI were identified as possible origin of replication. genome of Rhodobacter sphaeroides 2. 4. 1. Microbiology (submitted) and OriRII) of the Rhodobacter sphaeroides 2.4.1. Presented at the ASM-Texas putative origin of replication of the two Currently, these fragments were cloned into pLO1 Branch meeting (March 5-7, 2009): (Orville Wyass undergraduate student chromoosmes in Rhodobacter  Bavishi, A., L. Lin, K. Schroeder, and M. Choudhary (2009) Evidence of ancient Award, 2nd Place in poster presentation) sphaeroides, the fragments containing the (suicide vector), and recombinant clones were gene duplication in Rhodobacter sphaeroides 2. 4. 1. Microbiology (submitted) examined for their autonomous replication, which  Anne Peters, Kristen Schroeder, Lin Lin, and Madhusudan Choudhary (2009) pobable origin were cloned into plasmid will confirm the origin of replication on both  Bavishi, A., A. Abhishek, and M. Choudhary (2009) Complex prokaryotic genome Identification and characterization of gene duplication in Rhodobacter sphaeroides vector which does not replicate in R. chromosomes. structure: Rapid evolution of chromosome II. J. Bacteriol (submitted) 2.4.1. Presented at the ASM-Texas Branch meeting (March 5-7, 2009) sphaeroides. However, if the fragment  Choudhary, M., C. Mackenzie, T. J. Donohue, and S. Kaplan (2008) Purple  Kristen Schroeder, Anne Peters, Lin Lin, and Madhusudan Choudhary (2009) possess a true orgin, that would support bacterial genomics. In advances in photosynthesis and respiration. Vol. 28: 691-706. Structural-functional constraints on duplicated genes of the Rhodobacter the plasmid for the autonomous C. N. Hunter, F. Daldal, T. J. Beatty, and M. Thurnauer. (eds) Springer Verlag Inc. sphaeroides 2.4.1 Presented at the ASM-Texas Branch meeting (March 5-7, 2009) replication in R. sphaeroides cell. Tim Johnson UT-Dental School  Lin Lin, Kristen Schroeder, Anne Peters, and Madhusudan Choudhary (2009) Grants Structure-function constraints and gene expression analysis of the duplicated genes Luis Larios Identification of Chromosomal Origin of 2009 Enhancement Grant for Research (EGR), Sam Houston State University in the R. sphaeroides 2.4.1 Presented at Texas Genetics Society meeting (April 2-4, Role of Aurora Kinase in Cell Cycle post-baccalaureate Replication 2009) Cell cycle regulation in eukaryotes is G+C skew and Z-curve analyses were controlled by three Aurora kinase A, B, performed to predict the possible origin of and C. We have recently identified replication on chromosome I and II of homologs of Aurora kinase A and B in Rhodobacter sphaeroides. We have already Rhodobacter sphaeroides. My project identified 13 possible DNA sequence involves the construction of null mutaions fragments, several of these sequences also have in Auroral kinas A and B, and examine a number of DnaA and repA consensus motifs. their expression levels during the synchronized cell cycle.

Texas ASM, 2009 SHSU @ Texas ASM, 2009

Texas ASM, 2008 SHSU @ Texas ASM, 2009