Mitochondrial DNA Duplication, Recombination, and Introgression During Interspecific Hybridization
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Protein Splicing of Inteins Minireview and Hedgehog Autoproteolysis: Structure, Function, and Evolution
Cell, Vol. 92, 1±4, January 9, 1998, Copyright 1998 by Cell Press Protein Splicing of Inteins Minireview and Hedgehog Autoproteolysis: Structure, Function, and Evolution Francine B. Perler The Mechanism of Protein Splicing New England Biolabs, Inc. Splicing is extremely rapid and to date, precursors have Beverly, Massachusetts 01915 not been identified in native systems. The mechanism of protein splicing (Figure 2) has been extensively reviewed (Perler et al., 1997b; Shao and Kent, 1997). The process Protein splicing is a posttranslational editing process begins when the side chain hydroxyl or thiol of the con- that removes an internal protein fragment (intein) from served intein N-terminal Ser1 or Cys1 attacks the car- a precursor and ligates the external protein fragments bonyl (C 5 O) of the preceding amino acid, resulting in (exteins) to form the mature extein protein. Inteins, once an ester or thioester bond at the N-terminal splice site considered an oddity, are now known to be widely dis- (step 1, acyl rearrangement). This carbonyl is then at- tributed. The excised intein is also a stable protein that tacked by the hydroxyl/thiol group of the Ser/Thr/Cys can have homing endonuclease activity (reviewed in Bel- at the beginning of the C extein (the 11 residue) resulting fort and Roberts, 1997). Homing endonucleases are site- in N-terminal cleavage and formation of a branched in- specific enzymes that make double-strand breaks in termediate (step 2, transesterification). The branched intronless or inteinless alleles, initiating a gene conver- intermediate is resolved by cleavageof the peptide bond sion process that results in insertion of the mobile intron at the C-terminal splice site due to cyclization of the or intein gene. -
"Evolutionary Emergence of Genes Through Retrotransposition"
Evolutionary Emergence of Advanced article Genes Through Article Contents . Introduction Retrotransposition . Gene Alteration Following Retrotransposon Insertion . Retrotransposon Recruitment by Host Genome . Retrotransposon-mediated Gene Duplication Richard Cordaux, University of Poitiers, Poitiers, France . Conclusion Mark A Batzer, Department of Biological Sciences, Louisiana State University, Baton Rouge, doi: 10.1002/9780470015902.a0020783 Louisiana, USA Variation in the number of genes among species indicates that new genes are continuously generated over evolutionary times. Evidence is accumulating that transposable elements, including retrotransposons (which account for about 90% of all transposable elements inserted in primate genomes), are potent mediators of new gene origination. Retrotransposons have fostered genetic innovation during human and primate evolution through: (i) alteration of structure and/or expression of pre-existing genes following their insertion, (ii) recruitment (or domestication) of their coding sequence by the host genome and (iii) their ability to mediate gene duplication via ectopic recombination, sequence transduction and gene retrotransposition. Introduction genes, respectively, and de novo origination from previ- ously noncoding genomic sequence. Genome sequencing Variation in the number of genes among species indicates projects have also highlighted that new gene structures can that new genes are continuously generated over evolution- arise as a result of the activity of transposable elements ary times. Although the emergence of new genes and (TEs), which are mobile genetic units or ‘jumping genes’ functions is of central importance to the evolution of that have been bombarding the genomes of most species species, studies on the formation of genetic innovations during evolution. For example, there are over three million have only recently become possible. -
DE-Kupl: Exhaustive Capture of Biological Variation in RNA-Seq Data Through K-Mer Decomposition
Audoux et al. Genome Biology (2017) 18:243 DOI 10.1186/s13059-017-1372-2 METHOD Open Access DE-kupl: exhaustive capture of biological variation in RNA-seq data through k-mer decomposition Jérôme Audoux1, Nicolas Philippe2,3, Rayan Chikhi4, Mikaël Salson4, Mélina Gallopin5, Marc Gabriel5,6, Jérémy Le Coz5,EmilieDrouineau5, Thérèse Commes1,2 and Daniel Gautheret5,6* Abstract We introduce a k-mer-based computational protocol, DE-kupl, for capturing local RNA variation in a set of RNA-seq libraries, independently of a reference genome or transcriptome. DE-kupl extracts all k-mers with differential abundance directly from the raw data files. This enables the retrieval of virtually all variation present in an RNA-seq data set. This variation is subsequently assigned to biological events or entities such as differential long non-coding RNAs, splice and polyadenylation variants, introns, repeats, editing or mutation events, and exogenous RNA. Applying DE-kupl to human RNA-seq data sets identified multiple types of novel events, reproducibly across independent RNA-seq experiments. Background diversity is genomic variation. Polymorphism and struc- Successive generations of RNA-sequencing technologies tural variations within transcribed regions produce RNAs have bolstered the notion that organisms produce a highly with single-nucleotide variations (SNVs), tandem dupli- diverse and adaptable set of RNA molecules. Modern cations or deletions, transposon integrations, unstable transcript catalogs, such as GENCODE [1], now include microsatellites, or fusion events. These events are major hundreds of thousands of transcripts, reflecting pervasive sources of transcript variation that can strongly impact transcription and widespread alternative RNA processing. RNA processing, transport, and coding potential. -
Transcriptional Mechanisms of Resistance to Anti-PD-1 Therapy
Author Manuscript Published OnlineFirst on February 13, 2017; DOI: 10.1158/1078-0432.CCR-17-0270 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Transcriptional mechanisms of resistance to anti-PD-1 therapy Maria L. Ascierto1, Alvin Makohon-Moore2, 11, Evan J. Lipson1, Janis M. Taube3,4, Tracee L. McMiller5, Alan E. Berger6, Jinshui Fan6, Genevieve J. Kaunitz3, Tricia R. Cottrell4, Zachary A. Kohutek7, Alexander Favorov8,10, Vladimir Makarov7,11, Nadeem Riaz7,11, Timothy A. Chan7,11, Leslie Cope8, Ralph H. Hruban4,9, Drew M. Pardoll1, Barry S. Taylor11,12,13, David B. Solit13, Christine A Iacobuzio-Donahue2,11, and Suzanne L. Topalian5 From the 1Departments of Oncology, 3Dermatology, 4Pathology, 5Surgery, 6The Lowe Family Genomics Core, 8Oncology Bioinformatics Core, and the 9 Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine and Sidney Kimmel Comprehensive Cancer Center, Baltimore, MD 21287; the 10Laboratory of System Biology and Computational Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991, Moscow, Russia; and 2Pathology, 7Radiation Oncology, 11Human Oncology and Pathogenesis Program, 12Department of Epidemiology and Biostatistics, and the 13Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, New York NY 10065. MLA, AM-M, EJL, and JMT contributed equally to this work Running title: Transcriptional mechanisms of resistance to anti-PD-1 Key Words: melanoma, cancer genetics, immunotherapy, anti-PD-1 Financial Support: This study was supported by the Melanoma Research Alliance (to SLT and CI-D), the Bloomberg~Kimmel Institute for Cancer Immunotherapy (to JMT, DMP, and SLT), the Barney Family Foundation (to SLT), Moving for Melanoma of Delaware (to SLT), the 1 Downloaded from clincancerres.aacrjournals.org on October 2, 2021. -
MUC4/MUC16/Muc20high Signature As a Marker of Poor Prognostic for Pancreatic, Colon and Stomach Cancers
Jonckheere and Van Seuningen J Transl Med (2018) 16:259 https://doi.org/10.1186/s12967-018-1632-2 Journal of Translational Medicine RESEARCH Open Access Integrative analysis of the cancer genome atlas and cancer cell lines encyclopedia large‑scale genomic databases: MUC4/MUC16/ MUC20 signature is associated with poor survival in human carcinomas Nicolas Jonckheere* and Isabelle Van Seuningen* Abstract Background: MUC4 is a membrane-bound mucin that promotes carcinogenetic progression and is often proposed as a promising biomarker for various carcinomas. In this manuscript, we analyzed large scale genomic datasets in order to evaluate MUC4 expression, identify genes that are correlated with MUC4 and propose new signatures as a prognostic marker of epithelial cancers. Methods: Using cBioportal or SurvExpress tools, we studied MUC4 expression in large-scale genomic public datasets of human cancer (the cancer genome atlas, TCGA) and cancer cell line encyclopedia (CCLE). Results: We identifed 187 co-expressed genes for which the expression is correlated with MUC4 expression. Gene ontology analysis showed they are notably involved in cell adhesion, cell–cell junctions, glycosylation and cell signal- ing. In addition, we showed that MUC4 expression is correlated with MUC16 and MUC20, two other membrane-bound mucins. We showed that MUC4 expression is associated with a poorer overall survival in TCGA cancers with diferent localizations including pancreatic cancer, bladder cancer, colon cancer, lung adenocarcinoma, lung squamous adeno- carcinoma, skin cancer and stomach cancer. We showed that the combination of MUC4, MUC16 and MUC20 signature is associated with statistically signifcant reduced overall survival and increased hazard ratio in pancreatic, colon and stomach cancer. -
Applications of Xâ
Downloaded from genesdev.cshlp.org on June 30, 2009 - Published by Cold Spring Harbor Laboratory Press ‘IN n e-fr £ Development Targeted mutagenesis by homologous recombination in D. melanogaster Yikang S. Rong, Simon W. Titen, Heng B. Xie, et al. G e n e s D e v . 2002 16: 1568-1581 Access the most recent version at doi:10.1101/gad.986602 References This article cites 43 articles, 24 of which can be accessed free at: http://genesdev.cshlp.org/content/16/12/1568.fuN.html#ref-Nst-1 Article cited in: http://genesdev.cshlp.org/content/16/12/1568.full.html#related-urls Email alerting Receive free email alerts when new articles cite this article - sign up in the box at the service top right corner of the article or click here To subscribe to Genes & Development go to: http://genesdev.cshlp.org/subscriptions Cold Spring Harbor Laboratory Press Downloaded from genesdev.cshlp.org on June 30, 2009 - Published by Cold Spring Harbor Laboratory Press m utagenesis by recom bination in D . m elanogaster Yikang S. Rong,1,2,4 Simon W. Titen,1,2 Heng B. Xie,1,2 Mary M. Golic,1,2 Michael Bastiani,1 Pradip Bandyopadhyay,1 Baldomero M. Olivera,1 Michael Brodsky,3,5 Gerald M. Rubin,3 and Kent G. Golic1,2,6 departm ent of Biology, University of Utah, Salt Lake City, Utah 84112, USA; 2Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA; 3Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA Wc used a recently developed method to produce mutant alleles of five endogenous D r o s o p h ila genes, including the homolog of the p 5 3 tumor suppressor. -
1653.Full-Text.Pdf
Copyright 8 1997 by the Genetics Society of America Genetic Analysis of the Chlamydomonas &nhadtii I-CmI Mobile Intron Homing System in Escherichia coli Lenny M. Seligman,*’tKathryn M. Stephens,*Jeremiah H. Savaget and Raymond J. Monnat, Jr.* *Department of Pathology, University of Washington, Seattle, Washington 98195-7705 and ?Department of Biology, Pomona College, Claremont, California 91 71 1 Manuscript received May 22, 1997 Accepted for publication September 17, 1997 ABSTRACT We have developed and used a genetic selection system in Eschm’chia coli to study functional require- ments for homing site recognition and cleavage by a representative eukaryotic mobileintron endonucle- ase. The homing endonuclease, I-CreI, was originally isolated from the chloroplast of the unicellular green alga Chlamydomonas reinhardtii. I-CreI homing site mutants contained base pair substitutions or single basedeletions that altered the rate of homing site cleavage and/or product release. I-CreI endonu- clease mutants fell into six phenotypic classes that differed in in vivo activity, toxicity or genetic domi- nance. Inactivating mutations clustered in the N-terminal 60% of the I-CreI amino acid sequence, and two frameshift mutations were isolated that resulted in premature translation termination though re- tained partial activity. These mutations indicate that the N-terminal two-thirds ofthe I-CreI endonuclease is sufficient for homing site recognition and cleavage. Substitution mutations altered in four potential active site residues wereexamined DZON, Q47H or R70A substitutions inactivatedendonuclease activity, whereas S22A did not. The genetic approach we have taken complements phylogenetic and structural studies of mobile intron endonucleases and has provided new information on the mechanistic basis of I-CreI homing site recognition and cleavage. -
Xenopus Transgenic Nomenclature Guidelines
This document was last updated: October 23rd, 2014. Executive Summary: Xenopus Transgenic Nomenclature Guidelines These guidelines cover the naming of transgenic constructs developed and used in Xenopus research, and the naming of mutant and transgenic Xenopus lines . ● Only those transgenic (Tg) constructs that are supplied to other researchers, or are maintained by a lab or at a stock center, require standardized names following these guidelines. ● Xenbase will record and curate transgenes using these rules. ● Transgenic construct names are italicized following this basic formulae: Tg(enhancer or promoter:ORF/gene) ● Multiple cassette on the same plasmid are separated by a comma within the parenthesis. Tg(enhancer or promoter:ORF/gene, enhancer or promoter:ORF/gene) cassette 1 cassette 2 ● Transgenic Xenopus lines are named based on their unique combination of Tg constructs (in standard text) and indicate Xenopus species and the lab of origin: Xl.Tg(enhancer or promoter:ORF/gene, enhancer or promoter:ORF/gene)#ILAR lab code species cassette 1 cassette 2 lab code ● Xenopus research labs that generate Tg animals are identified using lab codes registered with the ILAR which maintains a searchable database for model organism laboratories. Section 1. Provides an overview for Naming Tg Constructs Section 2. Outlines specific rules for Naming Tg Constructs Section 3. Provides an overview for Naming Tg Xenopus lines and Xenopus lines with mutant alleles. Section 4. Provides guidelines for naming enhancer and gene trap constructs, and lines using these constructs. Section 5. Outlines rules for naming transposon-induced mutations and inserts. Section 6. Discusses disambiguation of wild type Xenopus strains versus transgenic and mutant Xenopus lines. -
Genome Organization/ Human
Genome Organization/ Secondary article Human Article Contents . Introduction David H Kass, Eastern Michigan University, Ypsilanti, Michigan, USA . Sequence Complexity Mark A Batzer, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA . Single-copy Sequences . Repetitive Sequences . The human nuclear genome is a highly complex arrangement of two sets of 23 Macrosatellites, Minisatellites and Microsatellites . chromosomes, or DNA molecules. There are various types of DNA sequences and Gene Families . chromosomal arrangements, including single-copy protein-encoding genes, repetitive Gene Superfamilies . sequences and spacer DNA. Transposable Elements . Pseudogenes . Mitochondrial Genome Introduction . Genome Evolution . Acknowledgements The human nuclear genome contains 3000 million base pairs (bp) of DNA, of which only an estimated 3% possess protein-encoding sequences. As shown in Figure 1, the DNA sequences of the eukaryotic genome can be classified sequences such as the ribosomal RNA genes. Repetitive into several types, including single-copy protein-encoding sequences with no known function include the various genes, DNA that is present in more than one copy highly repeated satellite families, and the dispersed, (repetitive sequences) and intergenic (spacer) DNA. The moderately repeated transposable element families. The most complex of these are the repetitive sequences, some of remainder of the genome consists of spacer DNA, which is which are functional and some of which are without simply a broad category of undefined DNA sequences. function. Functional repetitive sequences are classified into The human nuclear genome consists of 23 pairs of dispersed and/or tandemly repeated gene families that chromosomes, or 46 DNA molecules, of differing sizes either encode proteins (and may include noncoding (Table 1). -
Discovering and Characterizing New Homing Endonucleases for Genome Engineering
Discovering and Characterizing New Homing Endonucleases for Genome Engineering Kyle M. Jacoby A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy University of Washington 2013 Reading Committee: Dr. Andrew Scharenberg, Chair Dr. Stanley Fields Dr. Philip Bradley Program Authorized to Offer Degree: Molecular and Cellular Biology ©Copyright 2013 Kyle M. Jacoby ii Abstract University of Washington Discovering and Characterizing New Homing Endonucleases for Genome Engineering Kyle M. Jacoby Chair of the Supervisory Committee: Dr. Andrew Scharenberg Adjunct Associate Professor Department of Immunology LAGLIDADG Homing Endonucleases (LHEs) are a family of highly specific DNA- cutting enzymes capable of recognizing target sequences of ~20 bp. In many eukaryotes, including humans and yeast, double-strand breaks induced by LHEs stimulate repair by Homologous Recombination, which can be used to alter or repair a gene if the template is supplied in trans, and Non-Homologous End Joining, which can be used to knock out a gene. The potential for such precise genome editing would reduce worry about insertional mutagenesis or misregulation, as only the specific gene under its native promoter would be targeted. Thus, LHEs have drawn intense interest for their research, biotech and clinical applications. Methods for rational engineering of LHEs have been limited by a small number of high quality starting enzymes, and an extremely restricted understanding of how to modify them to iii create novel enzymes that efficiently cleave hybrid target sequences. Here I describe my attempts to address these limitations by using a homology-directed search method to acquire, characterize, and engineer a robust set of I-OnuI-related LHEs which recognize a diverse set of target sequences. -
Project Summary
"Genomic studies of chimeric mitochondria in cybrids from Solanaceae" 2009. Project Narrative Genome rearrangements, nucleotide substitutions, and the introduction of foreign DNA shape the mitochondrial genomes of eukaryotes and often have severe consequences for human health, as evidenced by several important mitochondrially-inherited diseases. In addition, the key experimental organism of this study (tobacco) is widely used as an expression system for biopharmaceutical production of vaccines, antibiotics, and a number of therapeutic proteins. Understanding the molecular mechanisms of mitochondrial DNA evolution is therefore of considerable importance to human health and disease. Project Summary The study of mitochondrial genome evolution, dynamics, uptake of foreign DNA, and interactions with the nuclear genome is essential to a deep understanding of the eukaryotic cell. Mitochondrial genomes provide an excellent system to increase our knowledge of genome rearrangements, chimeric genes, nuclear- cytoplasmic incompatibilities, and horizontal gene transfer (HGT). Plant mitochondria are unique in their propensity to acquire genes by HGT. The most pervasive example of HGT in eukaryotes involves the cox1 intron, which encodes a putative homing endonuclease that increases the frequency of the intron's fixation via horizontal transfer. We propose to study aspects of the interactions between two distinct mitochondrial genomes that recombine in a cybrid plant obtained by protoplast fusion experiments. During cybrid formation, the two mitochondrial parental types and their genomes (only one containing the cox1 intron) will fuse, resulting in a hybrid mitochondrial genome. We will establish some 20 cybrid lines derived from somatic crosses between cox1 intron-containing and -lacking species in order to address the following two aims: First, we will test the hypothesis that the cox1 intron encodes a functional homing endonuclease in plants, assess rates of intron colonization, and measure lengths of exonic coconversion tracts that accompany intron insertion. -
The Nicking Homing Endonuclease I-Basi Is Encoded by a Group I Intron in the DNA Polymerase Gene of the Bacillus Thuringiensis Phage Bastille
Nucleic Acids Research, 2003, Vol. 31, No. 12 3071±3077 DOI: 10.1093/nar/gkg433 The nicking homing endonuclease I-BasI is encoded by a group I intron in the DNA polymerase gene of the Bacillus thuringiensis phage Bastille Markus Landthaler and David A. Shub* University at Albany, SUNY, Department of Biological Sciences and Center for Molecular Genetics, Albany, NY, USA Downloaded from https://academic.oup.com/nar/article/31/12/3071/1395342 by guest on 28 September 2021 Received March 10, 2003; Revised and Accepted May 2, 2003 ABSTRACT occupies in the intron containing homolog, by a duplicative and unidirectional transfer. Here we describe the discovery of a group I intron in Homing endonucleases belong to four families based on the the DNA polymerase gene of Bacillus thuringiensis presence of well-conserved sequence motifs that are denoted phage Bastille. Although the intron insertion site is LAGLIDADG, His-Cys box, GIY-YIG and H-N-H, respect- identical to that of the Bacillus subtilis phages ively (3). Most homing endonucleases bind DNA in a SPO1 and SP82 introns, the Bastille intron differs sequence-speci®c fashion, recognizing large stretches of the from them substantially in primary and secondary intron-minus version of the gene in which they are inserted. In structure. Like the SPO1 and SP82 introns, the general, the recognition site comprises sequences of both Bastille intron encodes a nicking DNA endo- exons and the endonucleases generate a double-strand break nuclease of the H-N-H family, I-BasI, with a cleavage close to the intron insertion site (4).