Efficient Professional Protein and Antibody Platforms GPA33 Antibody Basic information: Catalog No.: UPA61087 Source: Rabbit Size: 50ul/100ul Clonality: polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: affinity purified by Protein A Useful Information: WB:1:500-2000 Applications: IHC-P:1:400-800 Reactivity: Human, Mouse, Rat Specificity: This antibody recognizes GPA33 protein. Immunogen: KLH conjugated synthetic peptide derived from human GPA33 151-280/319 Description: May play a role in cell-cell recognition and signaling. Uniprot: Q9JKA5 Human BiowMW: 33 KDa Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Tissue/cell: human gastric carcinoma; 4% Para- formaldehyde-fixed and paraffin-embedded; An- tigen retrieval: citrate buffer at 37℃ for 20 min; Incubation: Anti-GPA33 Polyclonal Antibody, Un- conjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB- staining

Tissue/cell: human gastric carcinoma; 4% Para- formaldehyde-fixed and paraffin-embedded; An- tigen retrieval: citrate buffer at 37℃ for 20 min; Incubation: Anti-GPA33 Polyclonal Antibody, Un- conjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB- staining

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Sample: Stomach (Rat) Lysate at 40 ug Primary: Anti-GPA33 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 30 kD

Paraformaldehyde-fixed, paraffin embedded at 37 ℃ for 30min; Antibody incubation with (GPA33) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]