DOCSLIB.ORG

Lab Instructional Demonstrations

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Lab Instructional Demonstrations Lab Instructional Demonstrations Lab Demos CIA Demonstration: Pipetting Page [1 of 2] A technique that you’re going to use over and over again in a laboratory is pipetting. The idea is that what we want to do is get a known volume of a liquid and then transfer it to another container. So I have here a collection of very beautiful, but very innocuous chemicals. This is just water with food coloring in it. And this is a pipette. Let me show you what the pieces are. We have two pieces of straight glass tube, and then there’s a bulge in the middle. This is where most the liquid is going to go. It has a tip, and I’ll come back to that in a second, and then there’s always a little mark that goes all the way around. There’s a mark there, and that’s the line that tells you when you’ve got the right volume. So, in other words, we’re going to bring liquid all the way up to this mark, and then the meniscus – remember, when we’re doing water, the meniscus is always this way. It’s lower in the middle than it is on the edges, and we want the bottom of the meniscus even with the mark on the pipette. So this is a pipette bulb. One thing that I’d like to caution you against is down jam this guy on really tight on to the end of the pipette. Besides the fact that it really ruins the piece of plastic here, there’s no reason to, because I’ll show you in a second that you really don’t want to have it jammed on really tight. Now, the properties of these pipettes, the volumetric pipettes, is that you don’t blow the liquid out of the tip. In other words, you fill them up to the line, and then you allow the liquid to drain out. But if there’s a little bit left at the tip, you leave it there. You can touch the tip to the side of your flask or the beaker to get that last drop that’s hanging off the end of the tip, but there’s going to be liquid left in the tip as a result of the fact that it just hangs there and you leave that there. So this is sort of important. So this is a 20 milliliter volumetric pipette, and I’m going to draw liquid up and, again, you put the pipette into the beaker. You can rest it on the bottom or you can pull it up a little bit, and then you take the pipette bulb and you squeeze it, and then you rest it gently on top of the top of the pipette, and as you gently release the bulb, liquid will be drawn up into the pipette. Now, if you have this jammed on really tight, have the bulb jammed on really tight, you won’t be able to remove it very easily. So let’s suppose now that it’s not entirely filled yet. I don’t know if you can see, but the liquid level is right here, and the bulb is off. So I can either let it all drain out, but in fact, all you need to do is cover the top of the pipette with your finger and then squeeze the bulb again, take your finger off, and continue to draw liquid up. You’re going to have to practice that a few times to get exactly the right feel. So what you do is draw the liquid so that it’s above the mark on the pipette, and then cover it with your index finger. Some people like to cover it with their thumb instead, but I like to cover it with my index finger of my left hand. And then what you have to do is get down so that you’re parallel with the mark on the pipette. So you have to drop your eye level to the level of the mark on the pipette, and then what you do is basically wiggle your index finger to let a little bit of air in. As you let a little bit of air in, the liquid is going to go down. If you overshoot, in other words, if the liquid goes down below the line, it’s no big deal. So I’m going to do that intentionally. Oh, darn, I’ve missed the line and all the liquid is here and I have to pull it up to the line again, so again, with my finger on top of the bulb, squeeze the bulb, and since I only need a little bit, you don’t even have to squeeze it very hard. Then put the bulb back on top of the pipette, and draw up above the line again. Then quickly pull it off. That’s why you don’t want it jammed on there, because you need to be able to take it on and off quickly. I get down to the right level, and what I’m doing is just sort of very gently bouncing my finger up and down, and trying to let little bits of air in, and the level of the pipette, the meniscus is making its way down the pipe – oops, went too far again. And sometimes you have to do it two or three times, but that’s no big deal. Oh, went too far again. My finger’s a little sticky this morning. Okay, that’s perfect. Now we’ll transfer it to a receiving flash. Again, you drain it until the liquid stops coming out, and you don’t blow out the liquid that’s retained in the tip. If there’s a drop hanging off the end of the pipette, you can go ahead and include that in your flask. So, for instance, if we’re getting ready to do a titration, this would be the first step in the titration, get a known quantity of the thing that you’re going to titrate. If you were going to do a dilution, the first step would be to get a known volume of the concentrated solution. Okay, so you can see that there’s some liquid left in the tip. That stays with the pipette, but you can go ahead and touch the tip to the sides. Okay, so that’s a volumetric pipette, and we delivered 20 milliliters. You’ll see that it says 20 milliliters on the pipette. So we delivered 20 milliliters to our Erlenmeyer flask. Now, this is a different kind of pipette. This is called a graduated pipette, and you can see that it has markings on it so that you can deliver different volumes, depending on what you want. These, in general, are less precise and less accurate, but if you’re just trying to get a certain volume that’s not exactly something convenient, like 20 or 50 – so they make these volumetric pipettes in 20 and 50 and 100 and whatever, but if you want something that’s in between, then you would need something like a graduated pipette. They work exactly the same way. You see that there are markings for zero, one, two, three, so if Lab Instructional Demonstrations Lab Demos CIA Demonstration: Pipetting Page [2 of 2] I wanted to deliver three milliliters, you fill it up to the zero, and then allow it to drain down to the three, and you’d be in business. So let’s go ahead and do that. Let’s say we want to add exactly three milliliters. We fill the pipette in exactly the same way. It doesn’t have to be exactly on zero, because what you’re trying to do, remember, is deliver a volume. So if it starts at 1/, then all you have to do is drain it to 3-1/10, and the difference between those two volumes is going to be exactly zero. What I’m finding for these pipettes is rather than bounce my finger around, just sort of try to raise my index finger ever so slightly so that it allows some air to go in. So it’s on zero. Now I’m going to drain out exactly three milliliters. You’ll have to get your eyes down to the level of the line. That’s exactly three milliliters. So I’ll put this back. So this is our solution that has 20 milliliters of blue and three milliliters of red. I see we’ve made a nice purple. This technique, pipetting, is going to show up over and over again when we do dilutions, when we do titrations, and so it’s a good idea to get some practice using this technique. .
Load more

Recommended publications
  • Laboratory Equipment Used in Filtration
    KNOW YOUR LAB EQUIPMENTS Test tube A test tube, also known as a sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. Beakers Beakers are used as containers. They are available in a variety of sizes. Although they often possess volume markings, these are only rough estimates of the liquid volume. The markings are not necessarily accurate. Erlenmeyer flask Erlenmeyer flasks are often used as reaction vessels, particularly in titrations. As with beakers, the volume markings should not be considered accurate. Volumetric flask Volumetric flasks are used to measure and store solutions with a high degree of accuracy. These flasks generally possess a marking near the top that indicates the level at which the volume of the liquid is equal to the volume written on the outside of the flask. These devices are often used when solutions containing dissolved solids of known concentration are needed. Graduated cylinder Graduated cylinders are used to transfer liquids with a moderate degree of accuracy. Pipette Pipettes are used for transferring liquids with a fixed volume and quantity of liquid must be known to a high degree of accuracy. Graduated pipette These Pipettes are calibrated in the factory to release the desired quantity of liquid. Disposable pipette Disposable transfer. These Pipettes are made of plastic and are useful for transferring liquids dropwise. Burette Burettes are devices used typically in analytical, quantitative chemistry applications for measuring liquid solution. Differing from a pipette since the sample quantity delivered is changeable, graduated Burettes are used heavily in titration experiments.
    [Show full text]
  • 10. Resource Requirement for Medical Laboratory Technology
    10. RESOURCE REQUIREMENT FOR MEDICAL LABORATORY TECHNOLOGY 10.1 PHYSICAL RESOURCES 10.1.1. SPACE REQUIREMENT The total space for lecture halls, laboratories may be worked out as per latest AICTE norms 10.1.2 LIST OF EQUIPMENT Note: All the glass wares to be of Borosil make The chemicals may be purchased of reputed make. Sr. Item Quantity No. LIST OF CHEMICALS FOR CHEMISTRY LABORATORY 1. Ferrous ammonium sulphate (Analytical reagent) 3 x 500 grams 2. Oxalic Acid (A.R) 2 x 500 grams 3. Potassium Permanganate (BDH) 3 x 500 grams 4. Sodium Hydroxide (BDH) 2 x 500 grams 5. Sulphuric Acid (AR) 5 litre 6. Hydrochloric Acid (AR) 5 litre 7. Sodium carbonate (A.R) 2 x 500 grams 8. Phenolphthalen (BDH/Merck) 10 grams 9. Methyl orange (SDH) 10 grams 10. Ethyl Alcohol 4 x 500 ml 11. Methyl Alcohol 500 ml 12. Nitric acid 5 litre 13. Glacial Acetic Acid (CP) 1 litre 14. Ammonium sulphate (BDH) 250 grams 15. Copper sulphate (BDH) 500 grams 16. Glucose (BDH) 500 grams 17. Sucrose (BDH) 500 grams 18. Acetone (BDH) 500 grams 19 Potassium Hydroxide (BDH) 2 x 500 grams 20. Iodine 1 x 500 grams 21. Calcium Chloride (BDH) 500 grams 93 Sr. Item Quantity No. 22 NaCl 500 gms 23. Ferric chloride (BDH) 500 grams 24. Starch pH 7.0 500 grams 25. Fructose 250 grams. 26. Gelatin 500 grams. 27. Benzaldehyde 500 ml 28. Ether 500 ml 29. Carbon Tetrachloride 500 ml 30. Ferrous Bicarbonate 500 grams 31. Sodium Bicarbonate 500 grams 32. Albumin 500 grams 33.
    [Show full text]
  • Practical Laboratory Skills for Molecular Biologists.Pdf 3.0 MB
    Practical laboratory skills for molecular biologists skills best practice training training best practice skills Practical laboratory skills for molecular biologists First Edition 2016 Co-authors Elena Sanchez Timothy Wilkes Contributors Nicholas Redshaw Acknowledgements With special thanks to Alison Woolford and Vicki Barwick for their help in the production of this guide. Production of this Guide was funded by the UK National Measurement System. First edition 2016 ISBN: 978-0-948926-28-0 This publication should be cited as: E Sanchez, T Wilkes, Practical laboratory skills for Copyright © 2016 LGC molecular biologists, LGC 2016. ISBN: 978-0-948926-28-0 Practical laboratory skills for molecular biologists OBJECTIVES OF THIS GUIDE 3 1 WORKING IN THE LABORATORY 4 1.1 Health and Safety issues 4 1.2 Laboratory environment 10 1.3 Equipment 14 1.4 Method selection 15 2 MEASURING VOLUME 16 2.1 Types of equipment available 16 2.2 Markings on equipment used for volumetric measurements 22 2.3 Selecting a suitable piece of equipment 24 2.4 Cleaning and maintenance of volumetric equipment 26 2.5 Checking the accuracy of volumetric equipment 27 2.6 Checklists for making measurements of volume 28 3 MEASURING MASS 33 3.1 Mass versus weight 33 3.2 Types of balance available 33 3.3 Selecting a suitable balance 34 4 QUANTIFICATION OF NUCLEIC ACID MATERIAL 41 4.1 Measurement of nucleic acid concentration using UV spectroscopy 41 4.2 Fluorescent based systems. 43 4.3 Gel electrophoresis 45 5 MEASURING PH 47 5.1 What is pH? 47 5.2 Equipment for measuring
    [Show full text]
  • BLAUBRAND® Volumetric Instruments and Density Bottles
    BLAUBRAND® Volumetric Instruments and Density Bottles Testing Instructions (SOP) March 2015 1. Introduction The standard DIN EN ISO 4787 describes both the design and the testing of the volumetric instruments of glass. The following Testing Instructions describe how to apply the ISO standard in practice. We recommend a testing every 1-3 years. The interval depends on the using of aggressive media and the cleaning procedure. These Instructions may also be used as a basis for the supervision of testing devices to DIN EN ISO 9001, DIN EN ISO 10012 and DIN EN ISO/IEC 17025. The test of the Density Bottles was effected on the basis of DIN EN ISO 4787. Meniscus adjustment with BLAUBRAND® Volumetric Instruments Meniscus adjustment Meniscus adjustment with ring mark with Schellbach stripe Read at the lowest point of the meniscus. Read at the point where the two arrows touch. Meniscus adjustment reflexion of liquid surface meniscus ring mark dark paper (p.e. black, blue) 2 2. Preparation for testing 2. Clear identification of the volumetric instrument to be tested Batch number, individual serial number, trademark, nominal volume and tolerances are directly printed ⇒ The test starts with a clear identification of the on every BLAUBRAND volumetric instruments. volumetric instrument in the test record. 2.1 Copy Test Record (see page 13) 2.2 Serial number/Identification number ⇒ Enter into Test Record All BLAUBRAND® volumetric instruments always carry a batch number, e.g., 13.04, or an individual serial number in the case of individual certificates, e.g., 13.040371 (year of production 2013, Batch No.
    [Show full text]
  • List of Equipment
    Mfg & Exporters: All types of Turnkey Projects, , Beverages, Mineral Water plant & OEM Spares List Of Equipment SR.NO Equipment Qty 1. Autoclave 1 Vertical Complete Heavy gauze with pressure gauze, Safety Valve, Stream Release Valve Size 30 X 30 cm 2. Digital Hot Air Ageing oven 1 Size 12” x 12” x 12” made of MS powder coated. Make- HB 3. Digital pH System : 1 Make- HB 4. Digital Incubator Bacteriological 2 12” X 12” X 12” made of MS powder coated. Make- HB 5. Water bath double walled 1 inner chamber of SS outer M.S powder coated with 6 hole with thermostatic control Make- HB 6. INNOCULATION CHAMBER, M.S POWDER COATED WITH 1 UV & 1 FLOROCENT LIGHT INBUILT 1 Make- HB 7. Automatic Hot Plate with 8 inch dia with Thermostat 1 Make-HB 8. Digital Spectrophotometer 1 Make- HB 9. Digital Turbidity meter 1 Make- HB 10. Digital Colony Counter 1 Make- HB 11. S.S. Filter holder 47 mm 1 (complete S.S) 12. Microscope student 1 Make-Labron 13. Magnetic Stirrer with Hot Plate with 1 lit capacity Top made of SS with Teflon Bed 1 Make- HB 14. Centrifuge ( 4 holders) 1 Make- HB 15. Single pan Balance ( pocket type) max: 0-500 gm, L.C: 0.1 gm 1 16. High pressure vacuum Pump 1 17. BOD Incubators inner complete S.S outer MS powder coated.( heavy) 2 Make – HB 18. Heating Mental 1 lit capacity 1 Make- HB 19. S.S distillation Unit 1 Address: 140, Tribhuvan Estate opp Road No 11 Kathwada GIDC Contact: +91 9726943972 Email: [email protected] Mfg & Exporters: All types of Turnkey Projects, , Beverages, Mineral Water plant & OEM Spares List Of Chemicals Sr.
    [Show full text]
  • J-Sil Price List
    An ISO 9001 : 2008 Certified Company TM J-SIL LABORATORY GLASSWARE Made from Borosilicate 3.3 Glass PRICE LIST 1st April, 2015 Manufactured by : J-SIL SCIENTIFIC INDUSTRIES MFRS. OF : SCIENTIFIC & LABORATORY GLASS APPARATUS 48-B, INDUSTR IAL ESTATE, NUNHAI, AGRA-282006 (INDIA) Phone : 2281967, 2281887 l Fax : 0562-2280186 E-mail : jsil @sancharnet.in, [email protected] Web : www.j-sil.com LABORATORY GLASS APPARATUS J-SIL 51. Chromatography Graduated, Screw Vials 9 mm wide Opening Type Cap Pkt Price/ Pkt A Clear Glass 2ml 100 PC 650.00 B Amber Glass 2ml 100 PC 700.00 56. Screw Cap with Septa for 9 mm Screw Vials (Closure) Cap Septa Pkt Price/ Pkt A Blue Non Slit 100 PC 750.00 B Blue Pre-Slit 100 PC 775.00 61. Clear Glass Screw Vials 9mm with Screw Blue Cap & PTFE-Silicon Septa (Combo) Cap Septa Pkt Price/ Pkt A 2 ml Non Slit 100 PC 1350.00 B 2 ml Pre-Slit 100 PC 1400.00 66. Amber Clear Glass Screw Vials 9mm with Screw Blue Cap & PTFE-Silicon Septa (Combo) Cap Septa Pkt Price/ Pkt A 2 ml Non Slit 100 PC 1400.00 B 2 ml Pre-Slit 100 PC 1450.00 LABORATORY GLASS APPARATUS J-SIL 71. Nylon Syringe Filter (Non Sterile) Packed in Transparent Plastic Jar DIA MICRON Pkt Price/ Pkt A 13 mm 0.22µm 100 1800.00 B 13 mm 0.45µm 100 1800.00 C 25 mm 0.22µm 100 1850.00 D 25 mm 0.45µm 100 1850.00 76. PVDF Syringe Filter (Non Sterile) Packed in Transparent Plastic Jar DIA MICRON Pkt Price/ Pkt A 13 mm 0.22µm 100 2800.00 B 13 mm 0.45µm 100 2800.00 C 25 mm 0.22µm 100 2850.00 D 25 mm 0.45µm 100 2850.00 81.
    [Show full text]
  • Accufine Life Sciences
    +91-8048861063 Accufine Life Sciences https://www.indiamart.com/rp-biotech/ Started in the year 2016, we “Accufine Life Sciences” has successfully emerged as a major Manufacturer, Trader, Exporter and Importer of high-quality Micropipette Tips, Microscope Slide, Swab Stick and many more. About Us Started in the year 2016, we “Accufine Life Sciences” has successfully emerged as a major Manufacturer, Trader, Exporter and Importer of high-quality Micropipette Tips, Microscope Slide, Swab Stick, Urine Containers, Lab Pipette, Vacutainer Tubes, Lab Disposables and many more. The basic material used in the manufacturing of these products are sourced from authentic vendors in the market and are rigorously tested on different parameters by our quality analysts assuring their quality. Also, our range has acknowledged due to the features like strong construction, long service life, and remarkably long life. We are supported by an ultra-modern infrastructure. Further, our strong customer reach approach is backed with the technological advancement that we have implemented at our infrastructure. Further, we assure that our lab glassware and plastic ware are suitably delivered to meet time schedules. We offer all our products in different sizes to meet the diversified demands of the clients. Under the headship of our mentor "Mr. Rakesh Singla", our organization is moving ahead in this cutting-edge competition. He has knowledge that helped us design immaculate lab glassware and plastic ware for the clients. For more information, please visit https://www.indiamart.com/rp-biotech/profile.html
    [Show full text]
  • Physicians Office Labs
    ® Select products for Physicians Office Labs www.globescientific.com ® Select products for Physicians Office Labs Table of Contents E.S.R. Systems .....................................2-5 Sedi-Rate™ Starter Kit Blood Collection ................................... 6-7 Capillary Tubes .....................................8-9 Specimen Containers .......................10-13 Thermometer Strips ...............................11 Patient Identification Labels ...................11 Specimen Transport Tubes .................. 13 Specimen Transport Bags .................... 14 Urinalysis Products ............................15-17 Centrifuge Tubes ..............................18-20 3467 — Sedi-Rate Starter Kit Test Tubes .........................................21-22 Sedi-Rate™ Starter Kit includes everything needed to perform safe and Caps for Test Tubes ....................6, 23-24 accurate Westergren ESR determinations. Kit includes: Racks for Test Tubes ...................... 25-27 100 Sedi-Rate pipettes and vials 100 Piercing funnels Microscope Slides/Accessories ....28-31 100 ESR transfer pipettes Pipettors ...........................................32-33 1 10-place rack with pipette support clamps 1 Leveling platform Pipette Tips ............................................. 34 Item # Description Unit 3467 Sedi-Rate Starter Kit 1 Kit Transfer Pipets ....................................... 35 2 Customer Service and Technical Support: 1 (201) 599-1400 Sedi-Rate™ E.S.R. System The use of Sedi-Rate™ eliminates splashing, spraying and aerosol
    [Show full text]
  • Laboratory Essentials
    LABORATORY ESSENTIALS COVID-19 SPECIAL WWW.WESTINDIGO.COM LABORATORY COVID-19 ESSENTIALS SPECIAL Test tube with screw cap Cylindrical test tubes, PS Cryotubes of PP 1. Autclavable and non-sterile. Supplied on demand Code Capacity Pack 2. Suitable for freezers, ultrafreezers or liquid BGD042 10 ml 1000 pcs 1. Code BGB021 and BGB023 are delivered nitrogen (vapour phase only). BGD043 10 ml b/200 sterile single packed. BGD044* 10 ml 750 pcs Code Capacity Pack Code Capacity Pack BGD045* 15 ml 750 pcs BGN001 2 ml B/500 BGB020 12 ml 1.500 pcs BGD046 15 ml 700 pcs BGN002 5 ml B/200 BGB021 12 ml 1.500 pcs BGD047 20 ml 500 pcs BGB022 30 ml 400 pcs BGD048 20 ml 500 pcs BGB023 30 ml 400 pcs Petri Dish aseptic, PS Sterile Sterile single packed PCR microtubes, PP * Supplied on demand Microtube strips for PCR, PP 1. Made of polystyrene. Edge of lip beveled to facilitate the sliding of the bottom. Aseptic and ventilated 1. Conical bottom, with flat lid. 2. Sterilizable in autoclave and can be also PACK: B/500 units 1. Conical bottom, lid strip included. frozen at -20ºC. 2. Sterilizable in autoclave and can be also Code Capacity 3. Supplied in bag of 1000 units. frozen at -20 ºC. BRB040 90 mm Code Capacity Pack: B/200 units BGN003 0,2 m Code Strip Capacity Micro centrifuge tube HQ BGN006 8 0,2 mL Feaces container, PS BGN007 12 0,2 mL Urine container, PS 1. All our HQ centrifuge tubes are biologically inert and certified and guaranteed to be 1.
    [Show full text]
  • Rheologic Studies on Synovial Fluid
    RHEOLOGIC STUDIES ON SYNOVIAL FLUID Milton G. Levine, David H. Kling J Clin Invest. 1956;35(12):1419-1427. https://doi.org/10.1172/JCI103399. Research Article Find the latest version: https://jci.me/103399/pdf RHEOLOGIC STUDIES ON SYNOVIAL FLUID By MILTON G. LEVINE AND DAVID H. KLING (From the Boyar-Kling Arthritis Clinic, Los Angeles, Calif.) (Submitted for publication March 28, 1956; accepted September 5, 1956) The relative viscosity of normal and pathologi- for most joint fluids; a 22 gauge is preferable for fluids cal synovial fluids fluctuates over a wide range, of low viscosity, and an 18 gauge for highly viscid fluids. and has been studied for a considerable period. The attachment of the needle to the pipette is made water-tight by a rubber sleeve, or by a cement seal, or The older literature is covered by Kling (1), and the delivery end of the pipette may be ground so that it the more recent by Ropes and Bauer (2) and by fits snugly into the hilt of the needle. Sundblad (3). Early investigators established, The viscometer is filled by suction above the zero by precipitation reactions with acetic acid, that mark, and the upper opening of the pipette is kept closed the high viscosity was due to a "mucin," assumed with a finger. The pipette is placed in a clamp holder and immersed in a constant temperature bath until the to be a combination of protein and carbohydrate. hilt of the hypodermic needle meets the water level. The In 1939, Meyer, Smyth, and Dawson (4) identi- measurement is started by releasing the finger, and the fied the polysaccharide as hyaluronic acid, and rate of flow is timed between two selected points on the found it to be responsible for the high viscosity.
    [Show full text]
  • CHM250 Calibration and Measurement Lab Green Profile
    CHM250 Calibration and Measurement Lab Green Profile Balance Calibration Introduction: Balances that are properly operated, calibrated and maintained are crucial for laboratory operations. The accuracy of all quantitative results is based on the ability of the balances to produce accurate and reproducible measurements within the strictest of quality control limits. For balances to work properly, they must be placed in a suitable environment. The work area should be relatively free from drafts and vibrations. Work surfaces must be level and rigid. The ideal balance location is free of significant temperature fluctuations. The balance must be cleaned and leveled properly before use. Any unclean balance surface could be a source of contamination. Balances must be clean, zeroed, leveled and free of all debris. Balance lids and doors should be closed and hoods replaced after use. Avoid dropping anything directly onto the balance pan as the shock may affect or damage the balance. The weight of a tarred vessel affects the accuracy and precision of a balance. The combined weights of the tarred vessel and material must be less than the maximum operating range of the balance. For example, if the analyst is weighing into a tarred 50 g beaker, the balance will behave as if it were loaded to greater than 50 g. Good laboratory procedure must be employed when weighing small amounts into large tarred amounts. Specific analytical methods may address this issue of choosing the appropriate balance and weighing techniques. A balance should not be used outside of its operating range or its limited usage range. When weighing, place the object directly in the center of the pan.
    [Show full text]
  • (1) Identification of the Common Laboratory Glassware, Pipettes and Equipment
    Biochemical Calculations BCH312 [Practical] BCH312 [Practical] ¨ Course Outline Title of the Experiments Identification of the common laboratory glassware, pipettes and 1 Equipment 2 Preparation of Solutions 3 Dilution of Solutions 4 Preparation of Different Buffer Solutions 5 Preparation of Buffer Solutions by Different Laboratory Ways 6 Titration of a weak acid with strong base 7 Titration curve of amino acids 8 Beer's- Lambert Law and Standard Curves BCH312 [Practical] ¨ Mark Distribution Tasks Marks Performance 2 Marks Report 4 Marks Quiz 5 Marks Final 14 Marks Total 25 Marks Final Exam Date 27 / 3 / 2019 BCH312 [Practical] ¨ How to write a Lab report? The laboratory reports should contain the following sections: 1) Cover page: Title, course number, student name. 2) Brief Introduction [ Theoretical background information ] 3) Objectives 4) Materials and Methods [ As in the lab sheet ] 5) Results [ Tables and Calculations ] 6) Discussion • In this section you are required to describe of what happened in the experiment [Principle] , explain your results and make conclusions by comparing your results to expected values • Even if you obtained unexpected results, the discussion section is the section to justify or explain the reasons why you have obtained such results. 7) Questions. 8) References. BCH312 [Practical] 5 (1) Identification of the common laboratory Glassware : a. Conical flasks and beakers. b. Graduated cylinders [measuring cylinder ]. c. Vo lu metric flasks. d. Burettes. e. Pipettes. 6 Glassware Conical flasks and beakers Graduated cylinders Volumetric flasks Burettes They are used for mixing, Used to measure the Used for preparing standard A burette delivers transporting, dissolving and volume of a liquid.
    [Show full text]