Feist & al. • Generic delimitations for Oxypolis and Ptilimnium TAXON 61 (2) • April 2012: 402–418 Revised generic delimitations for Oxypolis and Ptilimnium (Apiaceae) based on leaf morphology, comparative fruit anatomy, and phylogenetic analysis of nuclear rDNA ITS and cpDNA trnQ-trnK intergenic spacer sequence data Mary Ann E. Feist,1 Stephen R. Downie,2 Anthony R. Magee3,4 & Mei (Rebecca) Liu5 1 Illinois Natural History Survey, Prairie Research Institute, University of Illinois at UrbanaChampaign, Champaign, Illinois 61820, U.S.A. 2 Department of Plant Biology, University of Illinois at UrbanaChampaign, Urbana, Illinois 61801, U.S.A. 3 Compton Herbarium, South African National Biodiversity Institute, Private Bag X7, Claremont 7735, South Africa 4 Department of Botany and Plant Biotechnology, University of Johannesburg, P.O. Box 524, Auckland Park 2006, Johannesburg, South Africa 5 Department of Biology, Harbin Normal University, Hexing Road 50, Harbin 150080, People’s Republic of China Author for correspondence: Mary Ann E. Feist,
[email protected] Abstract A phylogenetic study of Oxypolis and Ptilimnium, two small genera of tribe Oenantheae (Apiaceae: subfamily Api- oideae), was carried out. Generic circumscriptions and infrageneric and infraspecific relationships were investigated through parsimony and Bayesian inference analyses of nuclear rDNA ITS and cpDNA trnQ5′rps16 and 3′rps165′trnK intergenic spacer sequences. Fruit anatomical characters were also examined and used in conjunction with leaf morphology to corroborate the results of the phylogenetic analyses. Each genus as currently delimited has both compound-leaved and rachis-leaved species. Results of the phylogenetic analyses show that neither Oxypolis nor Ptilimnium is monophyletic; each genus is split into two strongly supported clades that correspond to differences in leaf morphology within the groups.