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(19) United States (12) Patent Application Publication (10) Pub US 20060030489A1 (19) United States (12) Patent Application Publication (10) Pub. N0.: US 2006/0030489 A1 Northcott et al. (43) Pub. Date: Feb. 9, 2006 (54) AQUATIC PLANT PRODUCT AND METHOD Publication Classi?cation FOR MAKING GROWTH-SUSTAINING PLANT MATRIX (51) Int. Cl. A01N 39/02 (2006.01) (76) Inventors: Donald Owen Northcott, Cornwall A01H 9/00 (2006.01) (CA); Mark O. Hamran, Tea, SD (US) (52) Us. 01. .......................................... .. 504/323; 800/295 Correspondence Address: (57) ABSTRACT MARIO D. THERIAULT The invention consists of an aquatic plant product for resale, 812 HWY. 101 NASONWORTH having an extended shelf life, and a method for making the FREDERICTON, NB E3C 2B5 (CA) aquatic plant product. The aquatic plant product is made of a sealed container containing aquatic-plant-groWth-sustain (21) Appl. No.: 11/193,503 ing medium therein and an aquatic plant planted in that aquatic-plant-groWth-sustaining medium. The aquatic-plant (22) Filed: Aug. 1, 2005 groWth-sustaining medium contains a root-support material; at least one vitamin; sucrose; agar; distilled Water; micro Related US. Application Data nutrients containing iron; at least one hormone selected from a group consisting of auxin, naphthleneacetic acid, indole (60) Provisional application No. 60/599,985, ?led on Aug. 3-butric acid, cytokine, 2-isopentyladenine, 6 BenZylami 9, 2004. nopurine and any combination thereof. US 2006/0030489 A1 Feb. 9, 2006 AQUATIC PLANT PRODUCT AND METHOD FOR [0012] e) distilled Water; and MAKING GROWTH-SUSTAINING PLANT MATRIX [0013] f) at least one hormone selected from the list [0001] This application claims the bene?ts of provisional consisting of auxin, naphthleneacetic acid, indole-3-butric application Ser. No. 60/599,985, ?led on Aug. 9, 2004. acid, cytokine, 2-isopentyladenine, 6 benZylaminopurine and any combination thereof. FIELD OF THE INVENTION [0014] Basically, the sealed container contains a plant [0002] This invention pertains to retail packages of aquatic groWth-supporting medium and a sterile aquatic plant. The plants, and more speci?cally, it pertains to sealed containers plant-groWth-supporting medium is Water-based and by vir having groWth-sustaining media therein providing extended tue of being sealed, establishes Within the non-media portion shelf life for the plants therein. of the container interior, the air space, a relative humidity BACKGROUND OF THE INVENTION that is substantially 100%. This 100% humidity level closely approximates conditions aquatic plants experience While [0003] It is the dream of every ?sh hobbyist to decorate submerged, this then creates the conditions required for their living space With attractive, plant-?lled aquariums. successful groWth of aquatic plants. HoWever, it is expensive and labor-intensive for amateur hobbyists, or even professional breeders, to obtain and groW [0015] A sterile tissue-cultured aquatic plantlet When a variety of aquarium plants due to the fact that they require placed on a sterile plant-groWth-supporting medium in a specialiZed equipment to propagate. Moreover, aquatic plant sealed vessel establishes an equilibrium in this environment retailers experience signi?cant product losses With aquatic and as such only groWs at a rate supported by the factors that plant products, as the plants currently available must be control the metabolic processes. transplanted directly upon receipt of shipment from a pro [0016] Plants complete tWo processes, photosynthesis and vider or Will perish. Thus, What is needed is a shelf-stable respiration. In the photosynthesis process, Oxygen is aquatic plant product. released from the plant as a metabolic process. In respira tion, a plant absorbs Oxygen from the atmosphere and use SUMMARY OF THE INVENTION it to drive metabolic processes. So the cycling of a limited [0004] The present invention relates to an aquatic plant supply of Oxygen Within the closed system serves to regu product characteriZed by an extended shelf life, Which late plant groWth and in doing so it establishes the longevity product comprises at least one aquatic plant and a stable of the plant in the closed system. groWth-sustaining plant matrix, and to methods useful for [0017] This brief summary has been provided so that the generating same. For example, the present invention pro nature of the invention may be understood quickly. A more vides a sealed container, e.g., a test tube, containing therein complete understanding of the invention can be obtained by an aquatic plant, for example, a tissue-culture propagated reference to the folloWing detailed description of the pre aquatic plant, in a sterile environment and in a groWth ferred embodiment thereof. sustaining plant matrix. [0005] In one embodiment, the present invention provides DETAILED DESCRIPTION OF THE nutrient rich, non-toxic, foliar support matrix conditions to PREFERRED EMBODIMENT sustain an aquatic plant in a sealed container for an extended I. De?nitions period of time, for example, at least one year. An aquatic plant can be laboratory cultured under speci?c groWth [0018] The folloWing de?nitions are provided for speci?c parameters and conditioned for entry into a container that terms that are used in the folloWing Written description: provides the plant With suf?cient nutrients and air to survive [0019] As used in the speci?cation and claims, the singu un-aided and Without the addition of Water or other normal lar form “a,”“an” and “the” include plural references unless plant requirements. the context clearly dictates otherWise. For example, the term [0006] In one embodiment, the present invention provides “a plant” includes a plurality of plants, e.g., aquatic plant a tissue culture medium formulated to sustain the aquatic species. plant of interest, for example, a tissue-culture propagated [0020] An “aquatic plant” refers to members of the king aquatic plant, in a contained, sterile environment for up to doms Plantae and Protista that are capable of survival and/or one year. The tissue culture medium is speci?c for the plant, groWth in an aquatic environment, e.g., under Water or in a and has precise nutritive components, e.g., hormones, fer Wetland environment. tiliZers, amino acids, trace elements and minerals, such as fertiliZer and trace minerals Which aid in the groWth and root [0021] “Container” includes any suitable, sealable device formation, that sustain the individual plant. such as a tissue culture ?ask, tissue culture dish, test tube and the like. A container may be made of transparent [0007] For example, the medium according to the present material, such as glass, polystyrene and the like, or trans invention comprises: lucent material, such as polypropylene and the like. [0008] a) root-support material as described by inventors [0022] The terms “medium” and “media” are used inter Murashige& Skoog, Who are Well knoWn in this art; changeably With “substrate,”“matrix” and “formulation.” [0009] b) at least one vitamin; [0023] The phrase “shelf-stable” refers to the capability of the product of the invention to sustain the plant being stored [0010] c) sucrose; for an extended period of time, for example, for at least four [0011] d) agar; years, at a temperature such as room temperature, e.g., 50° US 2006/0030489 A1 Feb. 9, 2006 C.-85° C. In one embodiment of the invention, the shelf muskgrasses (Chara spp.), stoneWorts (Nitella spp.) and stable aquatic plant product of the invention can be stored at numerous vascular plants. Many submersed plants, such as room temperature for one to 4 years in the sterile container Widgeon-grass (Ruppia maritima), various pondWeeds Without having a deleterious effect on the ability of the (Potamogeton spp.), and tape-grass (Vallisneria spp.), are aquatic plant to survive, e.g., groW and/or proliferate, once native to the United States. transplanted into an aquatic environment. [0033] Submersed species face special problems obtaining [0024] “Sterile” or “sterile environment” refers to the light for photosynthesis and they must obtain carbon dioxide absence of prokaryotic, loWer eukaryotes species, and/or from the Water Where it is much less available than it is in pathogens. air. Submersed species invest much less energy into struc II. Exemplary Plants of the Invention tural support because they are supported by Water and Water accounts for about 95% of the Weight of these submersed [0025] The shelf-stable aquatic plant product of the inven species. tion includes a sterile, sealed container With at least one aquatic plant, the roots of Which are in contact With a [0034] Free ?oating macrophytes (plants that typically sustainable groWth medium that is tailored to meet the ?oat on or just under the Water surface With their roots in the nutritive needs of the plant(s) of interest. Water and not in sediment) are a diverse group of aquatic plants. Small free ?oating plants include duckWeeds (Lemna [0026] Any aquatic and/or Wetland plants can be used With spp.), mosquito fern (Azolla caroliniana) and Water fern the method of the present invention, such as any emergent, (Salvinia spp.). Larger surface ?oating plants include Water ?oating-leaved, submersed, and freely ?oating aquatic plant. hyacinth (Eichhornia crassipes), frog’s bit (Limnobium Aquatic macrophytes, by de?nition, are the macroscopic spongia). (large enough to be observed by the naked eye) forms of aquatic and Wetland plants found in Water bodies. The term [0035] Free ?oating species are entirely dependent on the aquatic macrophytes refers to a diverse group of aquatic Water for their nutrient supply. In fact, some (e.g., Water plants and encompasses ?oWering vascular plants, mosses, hyacinth) have been used in WasteWater treatment to remove ferns, and macroalgae. excess nutrients. [0027] Emergent macrophytes (plants that are rooted in [0036] Thus, the shelf-stable aquatic plant product of the substrate With the tops of the plant extending into the air) invention includes a sterile, sealed container With one groW on periodically inundated or submersed soils.
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