DOCSLIB.ORG

Univerzita Hradec Králové Přírodovědecká Fakulta Katedra

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Univerzita Hradec Králové Přírodovědecká Fakulta Katedra Univerzita Hradec Králové Přírodovědecká fakulta Katedra biologie Detekce mykotoxinů pomocí ELISA testu Diplomová práce Autor: Veronika, Dvořáková Studijní program: N1501 – Biologie Studijní obor: Systematická biologie a ekologie Vedoucí práce: Ing. Vladimír Dvořák, Ph.D. Hradec Králové květen 2015 Univerzita Hradec Králové Přírodovědecká fakulta Zadání diplomové práce Autor: Bc. Veronika Dvořáková Studijní program: N1501 Biologie Studijní obor: Systematická biologie a ekologie Název závěrečné práce: Detekce mykotoxinů pomocí ELISA testu Název práce v AJ: Using of ELISA test for detection of mycotoxins Cíl a metody práce: Cílem práce je na základě literární rešerše popsat metody měření mykotoxinů pomocí ELISA testu a zdokumentovat možné využitelné testovací sady. Dalším cílem práce je ověřit možnosti využití přístroje pro další metody měření. V praktické části bude zhotovena metodika pro použití přístroje ELISA, která bude následně ověřena při vlastním měření mykotoxinů ve studovaných vzorcích různého původu. Garantující pracoviště: Katedra biologie, Přírodovědecká fakulta Vedoucí práce: Ing. Vladimír Dvořák, Ph.D. Oponent: doc. RNDr. František Malíř, Ph.D. Datum zadání práce: 20. 11. 2013 Datum odevzdání závěrečné práce: Prohlášení: Prohlašuji, že jsem diplomovou práci vypracovala samostatně a že jsem v seznamu použité literatury uvedla všechny prameny, z kterých jsem vycházela. V Hradci Králové dne Jméno a příjmení PODĚKOVÁNÍ Ráda bych poděkovala Ing. Vladimíru Dvořákovi, Ph.D. za odborné vedení, cenné rady a trpělivost při řešení mé diplomové práce. Dále děkuji Ing. Tomáši Roubalovi ze Zdravotního ústavu Ústí nad Labem s pobočkou v Hradci Králové za odborné rady a zkušenosti, které jsem získala při práci v laboratoři. Anotace: DVOŘÁKOVÁ, V. Detekce mykotoxinů pomocí ELISA testu. Hradec Králové: Přírodovědecká fakulta Univerzity Hradec Králové, 2015. 64 s. Diplomová práce. Cílem práce je na základě literární rešerše popsat metody měření mykotoxinů pomocí ELISA testu a zdokumentovat možné využitelné testovací sady. Dalším cílem práce je ověřit možnosti využití přístroje pro další metody měření. V praktické části bude zhotovena metodika pro použití přístroje ELISA, která bude následně ověřena při vlastním měření mykotoxinů ve studovaných vzorcích různého původu. Klíčová slova mykotoxiny, ELISA test, ochratoxin A, testovací sady Annotation: DVOŘÁKOVÁ, V. Using of ELISA test for detection of mycotoxins. Hradec Králové: Faculty of Science, University of Hradec Kralove, 2015. 64 p. Diploma Thesis. The aim is to describe the methods of mycotoxin‘s measurements with using of ELISA test based on the literary research and to document an applicable assay kits. Another aim is to verify possibilities of using the device for further measurement procedures. The process for use of ELISA device will be determined in the practical part, which will be subsequently verified by mycotoxin’s measurements in the examined samples of various origins. Key words mycotoxins, ELISA test, ochratoxin A, assay kits Obsah 1 Úvod .................................................................................................................................................................... 9 2 Literární přehled ....................................................................................................................................... 11 2.1 Analýza mykotoxinů ........................................................................................................................ 11 2.1.1 Odběr a příprava vzorku ...................................................................................................... 11 2.1.2 Analýza .......................................................................................................................................... 11 2.2 ELISA test .............................................................................................................................................. 12 2.2.1 Přímá ELISA ................................................................................................................................ 13 2.2.2 Nepřímá ELISA .......................................................................................................................... 13 2.2.3 Kompetitivní ELISA ................................................................................................................. 13 2.2.4 Nekompetitivní ELISA ........................................................................................................... 14 2.3 Princip kompetitivní metody ELISA......................................................................................... 14 2.4 Základní části ELISA testu a přístrojové vybavení ............................................................ 15 2.5 Využití přístroje ELISA pro další metody měření .............................................................. 16 2.6 Další metody ........................................................................................................................................ 18 2.6.1 Chromatografie na tenké vrstvě (TLC) .......................................................................... 19 2.6.2 Vysokoúčinná kapalinová chromatografie (HPLC) ................................................. 19 2.6.3 Plynová chromatografie (GC) ............................................................................................. 19 2.6.4 EIA testy (enzymoimunoanalýza) .................................................................................... 20 2.6.5 Imunoafinitní chromatografie ........................................................................................... 20 2.6.6 Radioimunoanalýza (RIA).................................................................................................... 21 2.6.7 PCR (polymerázová řetězová reakce) ............................................................................ 21 2.7 Mykotoxiny........................................................................................................................................... 21 2.8 Ochratoxin A ........................................................................................................................................ 25 2.8.1 Druhy plísní produkující ochratoxin A .......................................................................... 26 2.9 Léčivé rostliny, koření .................................................................................................................... 27 2.10 Lékořice ............................................................................................................................................. 29 2.10.1 Nařízení komise EU č.105/2010 .................................................................................. 31 3 Metodika ........................................................................................................................................................ 33 3.1 Stanovení ochratoxinu pomocí sady RIDASCREEN .......................................................... 33 3.1.1 Příprava vzorku lékořice ...................................................................................................... 34 3.2 Metodika práce s testovací sadou ............................................................................................. 35 3.3 Metodika práce s automatickou promývačkou stripů .................................................... 39 4 Výsledky ......................................................................................................................................................... 44 5 Diskuze ........................................................................................................................................................... 48 6 Závěr ................................................................................................................................................................ 52 7 Seznam Literatury ..................................................................................................................................... 54 8 Seznam obrázků, tabulek a grafu ....................................................................................................... 62 9 Přílohy............................................................................................................................................................. 63 1 Úvod V našem životním prostředí se setkáváme s různými patogenními plísněmi, jejichž sekundární produkty tzv. mykotoxiny, mohou mít závažný vliv na zdraví člověka. Způsobují mykózy a alergie, zasahují různé orgány a orgánové soustavy. Diplomová práce je zaměřena na detekci mykotoxinů. Je důležité se tímto tématem zabývat, protože produkty plísní se dostávají do potravin a krmiv během zpracování a skladování a setkat se s nimi může každý člověk či hospodářské zvíře. Významné je stanovení maximálního limitu denního přívodu mykotoxinů, které je v některých zemích stále nedostatečné. Tato práce je zaměřena na detekci a měření mykotoxinu ochratoxinu A, který je produkován plísněmi rodu Aspergillus a Penicillium. Na lidský organismus může mít nefrotoxické, hepatotoxické, karcinogenní, imunosupresivní nebo teratogenní účinky, které jsou velmi závažné. Teoretická část práce se především zabývá měřením mykotoxinů s využitím ELISA testu. Poměrně podrobně je popsán průběh analýzy mykotoxinů, jehož základem je správný odběr a příprava vzorku. Dále je prezentován princip měření pomocí ELISA přístroje a vysvětlen základ, týkající se ELISA testování.
Load more

Recommended publications
  • Characterization of UDP-Glucose Dehydrogenase Isoforms in the Medicinal Legume Glycyrrhiza Uralensis
    Plant Biotechnology 38, 205–218 (2021) DOI: 10.5511/plantbiotechnology.21.0222a Original Paper Characterization of UDP-glucose dehydrogenase isoforms in the medicinal legume Glycyrrhiza uralensis Ayumi Kawasaki, Ayaka Chikugo, Keita Tamura, Hikaru Seki, Toshiya Muranaka* Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan * E-mail: [email protected] Tel: +81-6-6879-7423 Fax: +81-6-6879-7426 Received June 15, 2020; accepted February 22, 2021 (Edited by S. Takahashi) Abstract Uridine 5′-diphosphate (UDP)-glucose dehydrogenase (UGD) produces UDP-glucuronic acid from UDP- glucose as a precursor of plant cell wall polysaccharides. UDP-glucuronic acid is also a sugar donor for the glycosylation of various plant specialized metabolites. Nevertheless, the roles of UGDs in plant specialized metabolism remain poorly understood. Glycyrrhiza species (licorice), which are medicinal legumes, biosynthesize triterpenoid saponins, soyasaponins and glycyrrhizin, commonly glucuronosylated at the C-3 position of the triterpenoid scaffold. Often, several different UGD isoforms are present in plants. To gain insight into potential functional differences among UGD isoforms in triterpenoid saponin biosynthesis in relation to cell wall component biosynthesis, we identified and characterized Glycyrrhiza uralensis UGDs (GuUGDs), which were discovered to comprise five isoforms, four of which (GuUGD1–4) showed UGD activity in vitro. GuUGD1–4 had different biochemical properties, including their affinity for UDP-glucose, catalytic constant, and sensitivity to feedback inhibitors. GuUGD2 had the highest catalytic constant and highest gene expression level among the GuUGDs, suggesting that it is the major isoform contributing to the transition from UDP-glucose to UDP-glucuronic acid in planta.
    [Show full text]
  • Specificity in Legume-Rhizobia Symbioses
    International Journal of Molecular Sciences Review Specificity in Legume-Rhizobia Symbioses Mitchell Andrews * and Morag E. Andrews Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Lincoln 7647, New Zealand; [email protected] * Correspondence: [email protected]; Tel.: +64-3-423-0692 Academic Editors: Peter M. Gresshoff and Brett Ferguson Received: 12 February 2017; Accepted: 21 March 2017; Published: 26 March 2017 Abstract: Most species in the Leguminosae (legume family) can fix atmospheric nitrogen (N2) via symbiotic bacteria (rhizobia) in root nodules. Here, the literature on legume-rhizobia symbioses in field soils was reviewed and genotypically characterised rhizobia related to the taxonomy of the legumes from which they were isolated. The Leguminosae was divided into three sub-families, the Caesalpinioideae, Mimosoideae and Papilionoideae. Bradyrhizobium spp. were the exclusive rhizobial symbionts of species in the Caesalpinioideae, but data are limited. Generally, a range of rhizobia genera nodulated legume species across the two Mimosoideae tribes Ingeae and Mimoseae, but Mimosa spp. show specificity towards Burkholderia in central and southern Brazil, Rhizobium/Ensifer in central Mexico and Cupriavidus in southern Uruguay. These specific symbioses are likely to be at least in part related to the relative occurrence of the potential symbionts in soils of the different regions. Generally, Papilionoideae species were promiscuous in relation to rhizobial symbionts, but specificity for rhizobial genus appears to hold at the tribe level for the Fabeae (Rhizobium), the genus level for Cytisus (Bradyrhizobium), Lupinus (Bradyrhizobium) and the New Zealand native Sophora spp. (Mesorhizobium) and species level for Cicer arietinum (Mesorhizobium), Listia bainesii (Methylobacterium) and Listia angolensis (Microvirga).
    [Show full text]
  • Chloroplast Phylogenomics and Biogeography of Liquorice (Leguminosae: Glycyrrhiza)
    Prime Archives in Plant Sciences Book Chapter Chloroplast phylogenomics and biogeography of liquorice (Leguminosae: Glycyrrhiza) Lei Duan1,7, AJ Harris1, Li-Yan Mao2, Zhi-Rong Zhang3, Emine Arslan4, Kuddisi Ertuğrul4, Phan Ke Loc5, Hiroaki Hayashi6, Jun Wen7* and Hong-Feng Chen1* 1Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, China 2Guangxi Subtropical Crops Research Institute, China 3Germplasm Bank of Wild Species in Southwest China, Kunming Institution of Botany, Chinese Academy of Sciences, China 4Department of Biology, Faculty of Science, Selçuk University, Turkey 5Faculty of Biology, Department of Botany and HNU, VNU Hanoi University of Science (HUS), Vietnam 6Laboratory of Natural Products Chemistry, College of Pharmaceutical Sciences, Ritsumeikan University, Japan 7Department of Botany, National Museum of Natural History, MRC 166, Smithsonian Institution, USA *Corresponding Authors: Jun Wen, Department of Botany, National Museum of Natural History, MRC 166, Smithsonian Institution, Washington D.C. 20013-7012, U.S.A Hong-Feng Chen, Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China Published July 09, 2020 This Book Chapter is a republication of an article published by Lei Duan, et al. at Frontiers in Plant Science in June 2020. (Duan 1 www.videleaf.com Prime Archives in Plant Sciences L, Harris AJ, Su C, Zhang Z-R, Arslan E, Ertuğrul K, Loc PK, Hayashi H, Wen J and Chen H-F (2020) Chloroplast Phylogenomics Reveals the Intercontinental Biogeographic History of the Liquorice Genus (Leguminosae: Glycyrrhiza). Front. Plant Sci. 11:793. doi: 10.3389/fpls.2020.00793) How to cite this book chapter: Lei Duan, AJ Harris, Li-Yan Mao, Zhi-Rong Zhang, Emine Arslan, Kuddisi Ertuğrul, Phan Ke Loc, Hiroaki Hayashi, Jun Wen, Hong-Feng Chen.
    [Show full text]
  • Traditional Uses, Bioactive Chemical Constituents, and Pharmacological and Toxicological Activities of Glycyrrhiza Glabra L. (Fabaceae)
    biomolecules Review Traditional Uses, Bioactive Chemical Constituents, and Pharmacological and Toxicological Activities of Glycyrrhiza glabra L. (Fabaceae) 1,2, , 1, 3 Gaber El-Saber Batiha * y , Amany Magdy Beshbishy y, Amany El-Mleeh , Mohamed M. Abdel-Daim 4,5 and Hari Prasad Devkota 6 1 National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro 080-8555, Hokkaido, Japan; [email protected] 2 Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt 3 Department of Pharmacology, Faculty of Veterinary Medicine, Menoufia University, Menofia Governorate 32511, Egypt; [email protected] 4 Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; [email protected] 5 Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt 6 Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Chuo-ku, Kumamoto City 862-0973, Kumamoto, Japan; [email protected] * Correspondence: [email protected] or [email protected]; Tel./Fax: +20-45-271-6024 These authors contributed equally to this work. y Received: 31 January 2020; Accepted: 18 February 2020; Published: 25 February 2020 Abstract: Traditional herbal remedies have been attracting attention as prospective alternative resources of therapy for diverse diseases across many nations. In recent decades, medicinal plants have been gaining wider acceptance due to the perception that these plants, as natural products, have fewer side effects and improved efficacy compared to their synthetic counterparts. Glycyrrhiza glabra L. (Licorice) is a small perennial herb that has been traditionally used to treat many diseases, such as respiratory disorders, hyperdipsia, epilepsy, fever, sexual debility, paralysis, stomach ulcers, rheumatism, skin diseases, hemorrhagic diseases, and jaundice.
    [Show full text]
  • Supplementary Table S1. Species-Specific Information on Plant Functional Traits and Water Sources
    Supplementary Table S1. Species-specific information on plant functional traits and water sources Water source water Leaf Life DBH LAI Height Isotope Species Family Season Climate extraction References phenology Form (cm) (m2/m2) (m) Soil type Ground method (depth) Rock Rain Stream Fog Water cm Eucalyptus Myrtaceae E T 14 1 na 20- 60 GW D T AD D Peter et al., 1993 largiflorens Eucalyptus Myrtaceae E T 14 2 na 20-90 GW D T AD D Peter et al., 1993 largiflorens Eucalyptus Myrtaceae E T 14 3 na 30-70 GW D T AD D Peter et al., 1993 largiflorens Juniperus oxycedrus Cupressaceae E T na na na GW D M CD S Valentini et al.,1995 Quercus Fagaceae D T na na na GW D M CD S Valentini et al.,1995 pubescens Quercus cerris Fagaceae D T na na na GW D M CD S Valentini et al.,1995 Quercus ilex Fagaceae E T na na na R D M CD S Valentini et al.,1995 Pistacia lentiscus Anacardiaceae E T na na na R D M CD S Valentini et al.,1995 Phillyrea angustifolia Oleaceae E S na na na R D M CD S Valentini et al.,1995 Pinus sylvestris Pinaceae E T na na na R D M CD S Valentini et al.,1995 Larix decidua Pinaceae D T na na na GW D M CD S Valentini et al.,1995 Larix sibirica Pinaceae D T na 2.7 20 40 D SA CD D Li et al., 2007 Larix sibirica Pinaceae D T na 2.7 20 8 D SA CD D Li et al., 2007 Larix sibirica Pinaceae D T na 2.7 20 21 D SA CD D Li et al., 2007 Juniperus ashei Cupressaceae E T na na na <10 W TM VD D McCole and Stern, 2007 Juniperus ashei Cupressaceae E T na na na S D TM VD D McCole and Stern, 2007 Rademachera sinica Bignoniaceae SD T na na na S D A CD S Nie et al.,
    [Show full text]
  • Meyan) Cinsinin Revizyonu
    T.C. SELÇUK ÜNİVERSİTESİ FEN BİLİMLERİ ENSTİTÜSÜ TÜRKİYE’DE YAYILIŞ GÖSTEREN GLYCYRRHIZA L. (MEYAN) CİNSİNİN REVİZYONU Özlem ÇETİN DOKTORA TEZİ Biyoloji Anabilim Dalı Nisan-2015 KONYA Her Hakkı Saklıdır ÖZET DOKTORA TEZİ TÜRKİYE’DE YAYILIŞ GÖSTEREN GLYCYRRHIZA L. (MEYAN) CİNSİNİN REVİZYONU Özlem ÇETİN Selçuk Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı Danışman: Prof. Dr. Ahmet DURAN 2015, 305 Sayfa Jüri Prof. Dr. Ahmet DURAN Prof. Dr. Hüseyin DURAL Prof. Dr. Yavuz BAĞCI Doç. Dr. Esra MARTİN Doç. Dr. Ekrem DÜNDAR Bu çalışmada, ülkemizde doğal olarak yayılış gösteren Glycyrrhiza L. (Fabaceae) cinsi taksonları morfolojik, sitotaksonomik, palinolojik, moleküler ve nümerik çalışmalara dayalı veriler kullanılarak revizyonu gerçekleştirildi. Tüm taksonların daha kullanışlı teşhis anahtarları ve kapsamlı betimleri yapıldı. Ayrıca habitat özellikleri, yayılış haritaları ve fotoğrafları verildi. Hayat formu ve IUCN kategorileri değerlendirildi. Glycyrrhiza taksonlarının kromozom sayıları belirlendi. G. asymetrica türünün somatik kromozom sayısı 2n=14, Glycyrrhiza glabra, G. echinata subsp. echinata, G. echinata subsp. macedonica, G. flavescens subsp. flavescens, G. flavescens subsp. antalyensis, G. asymmetrica taksonlarının somatik kromozom sayısı ise 2n=16’dır. Görüntü Analiz Sistemi (Bs200ProP) aracılığı ile karyotip analizleri yapıldı. Tohum yüzey ornemantasyonu taramalı elektron mikroskobu ile incelendi. Ayrıca polenler ışık ve elektron mikroskobu ile incelendi. Farklı lokalitelerden toplanan örneklerinden DNA izolasyonları
    [Show full text]
  • I. Le Reflux Gastro-Oesophagien (Rgo)
    THESE PRESENTEE ET PUBLIQUEMENT SOUTENUE DEVANT LA FACULTE DE PHARMACIE DE MARSEILLE LE LUNDI 21 OCTOBRE 2019 PAR Mme BACCAM ADELINE Née le 23 NOVEMBRE 1993 à MARSEILLE. EN VUE D’OBTENIR LE DIPLOME D’ETAT DE DOCTEUR EN PHARMACIE TITRE : LES PROPRIETES DE L’ALOES, DE LA GUIMAUVE ET DE LA REGLISSE DANS LA PRISE EN CHARGE DU REFLUX GASTRO-OESOPHAGIEN JURY : Président : Mme OLLIVIER Evelyne Membres : Mme BAGHDIKIAN Béatrice Mr ASTIER Pierre Université d’Aix-Marseille – Faculté de Pharmacie – 27 bd Jean Moulin – CS 30064 - 13385 Marseille cedex 05 - France Tél. : +33 (0)4 91 83 55 00 - Fax : +33 (0)4 91 80 26 12 27 Boulevard Jean Moulin – 13385 MARSEILLE Cedex 05 Tel. : 04 91 83 55 00 – Fax : 04 91 80 26 12 ADMINISTRATION : Doyen : Mme Françoise DIGNAT-GEORGE Vice-Doyens : M. Jean-Paul BORG, M. François DEVRED, M. Pascal RATHELOT Chargés de Mission : Mme Pascale BARBIER, M. David BERGE-LEFRANC, Mme Manon CARRE, Mme Caroline DUCROS, Mme Frédérique GRIMALDI Conseiller du Doyen : M. Patrice VANELLE Doyens honoraires : M. Jacques REYNAUD, M. Pierre TIMON-DAVID, M. Patrice VANELLE Professeurs émérites : M. José SAMPOL, M. Athanassios ILIADIS, M. Jean-Pierre REYNIER, M. Henri PORTUGAL Professeurs honoraires : M. Guy BALANSARD, M. Yves BARRA, Mme Claudette BRIAND, M. Jacques CATALIN, Mme Andrée CREMIEUX, M. Aimé CREVAT, M. Bernard CRISTAU, M. Gérard DUMENIL, M. Alain DURAND, Mme Danielle GARÇON, M. Maurice JALFRE, M. Joseph JOACHIM, M. Maurice LANZA, M. José MALDONADO, M. Patrick REGLI, M. Jean- Claude SARI Chef des Services Administratifs : Mme Florence GAUREL Chef de Cabinet : Mme Aurélie BELENGUER Responsable de la Scolarité : Mme Nathalie BESNARD DEPARTEMENT BIO-INGENIERIE PHARMACEUTIQUE Responsable : Professeur Philippe PICCERELLE PROFESSEURS BIOPHYSIQUE M.
    [Show full text]
  • Formulário De Fitoterápicos Da Farmacopeia Brasileira, 2ª Edição Ii
    Formulário de Fitoterápicos 2ª EDIÇÃO Farmacopeia Brasileira Agência Nacional de Vigilância Sanitária - Anvisa Agência Nacional de Vigilância Sanitária Formulário de Fitoterápicos 2ª edição Farmacopeia Brasileira Brasília 2021 Formulário de Fitoterápicos da Farmacopeia Brasileira, 2ª edição ii Copyright © 2021. Agência Nacional de Vigilância Sanitária É permitida a reprodução parcial ou total desta obra, desde que citada a fonte. É vedada a impressão, distribuição, reprodução desta obra para fins comerciais sem a prévia e expressa anuência da Anvisa. Disponível em: <https://www.gov.br/anvisa/pt-br> Diretor-Presidente Antônio Barra Torres Diretores Alex Machado Campos Cristiane Rose Jourdan Gomes Meiruze Sousa Freitas Romison Rodrigues Mota Gerência de Laboratórios de Saúde Pública Nélio Cézar de Aquino Coordenador da Farmacopeia Arthur Leonardo Lopes da Silva Aprovado pela Resolução da Diretoria Colegiada – RDC nº 463, de 27 de janeiro de 2021. Formulário de Fitoterápicos da Farmacopeia Brasileira, 2ª edição iii SUMÁRIO 1 HISTÓRICO.................................................................................................................................... 1 2 FARMACOPEIA BRASILEIRA .................................................................................................. 4 3 GENERALIDADES ........................................................................................................................ 6 TÍTULO ..........................................................................................................................................
    [Show full text]
  • Toxicité Et Utilisation De Quelques Fabaceae Alimentaires Et Médicinales Anne-Claire Petit
    Toxicité et utilisation de quelques Fabaceae alimentaires et médicinales Anne-Claire Petit To cite this version: Anne-Claire Petit. Toxicité et utilisation de quelques Fabaceae alimentaires et médicinales. Sciences pharmaceutiques. 2011. hal-01739149 HAL Id: hal-01739149 https://hal.univ-lorraine.fr/hal-01739149 Submitted on 20 Mar 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. AVERTISSEMENT Ce document est le fruit d'un long travail approuvé par le jury de soutenance et mis à disposition de l'ensemble de la communauté universitaire élargie. Il est soumis à la propriété intellectuelle de l'auteur. Ceci implique une obligation de citation et de référencement lors de l’utilisation de ce document. D'autre part, toute contrefaçon, plagiat, reproduction illicite encourt une poursuite pénale. Contact : [email protected] LIENS Code de la Propriété Intellectuelle. articles L 122. 4 Code de la Propriété Intellectuelle. articles L 335.2- L 335.10 http://www.cfcopies.com/V2/leg/leg_droi.php http://www.culture.gouv.fr/culture/infos-pratiques/droits/protection.htm
    [Show full text]
  • T.C. Gazi Üniversitesi Sağlik Bilimleri Enstitüsü
    T.C. GAZİ ÜNİVERSİTESİ SAĞLIK BİLİMLERİ ENSTİTÜSÜ Farmakognozi Anabilim Dalı Fitoterapi Yüksek Lisans Programı çerçevesinde yürütülmüş olan bu çalışma aşağıdaki jüri tarafından Yüksek Lisans Tezi olarak kabul edilmiştir. Tez Savunma Tarihi: 30 / 12 / 2013 I İÇİNDEKİLER Kabul ve Onay…………………………………………………………...….......I İçindekiler………………………………………………………………...........II Şekiller, Grafikler………….…..…………………...……………………...…..V Resimler.……………………………………………..…………………….......VI Tablolar.……………………………….……………………….......................VII Semboller, Kısaltmalar ………………………………………………….....VIII 1. GİRİŞ VE AMAÇ……….………………….……………………...………….1 2. GENEL BİLGİLER…….………………………………..…………..…….....3 2.1.Tarihçe.…….……………………………………………………….............4 2.2.Glycyrrhiza glabra L.’nin Taksonomideki Yeri……….….…...................6 2.3. Leguminosae (baklagiller) Familyasının Genel Özellikleri……………..7 2.4. Papilionaceae (Fabaceae) Alt Familyası…………………………..........9 2.5. Glycyrrhiza Cinsinin Genel Özellikleri …………………….....………...10 2.6. Glycyrrhiza glabra Türünün Genel Özellikleri…………….……………11 2.6.1. Radix liquiritiae………………………..………………………………...11 2.6.2. Succus Liquiritiae…………………………………………………….…12 2.6.3. Glycyrrhiza glabra’nın Kayıtlı Olduğu farmakopeler……………….12 2.7. Halk İlacı Olarak Kullanılışı……………………………………..……….14 2.8. Kimyasal Yapısı……………………………………………………….....14 2.8.1. Saponinler…………………………………………………………........14 2.8.2 Flavonoitler ve Kalkonlar…………………………….…………………15 2.8.3. İzoflavonlar......................................................................................17 2.8.4. Kumarinler……….……………………………………………………...18
    [Show full text]
  • Characterization of Flavonoids from Albizia Amara Leaves and Their Biological Activity on Microbes
    Sudan University of Science and Technology College of Graduate Studies Characterization of Flavonoids from Albizia amara Leaves and their Biological Activity on Microbes ﺗﻮﺻﯿﻒ ﻓﻼﻓﻮﻧﯿﺪات أوراق ﻧﺒﺎت اﻟﻌﺮد وﺗﺄﺛﯿﺮھﺎ اﻟﺤﯿﻮي ﻋﻠﻲ اﻟﻤﯿﻜﺮوﺑﺎت A Thesis Submitted in Fulfillment of the Requirements of the Ph.D. Degree in Chemistry By: Munged Ibrahim Elsedig Mohamed (B.Sc. in Chemistry and Biology, M.Sc. in Chemistry) Supervisor: Prof. Dr. Mohamed Abdel Karim Mohammed October, 2016 اﻹﺳﺘﻔﺘﺎﺣﯿﺔ �ﺴﻢ ﷲ اﻟﺮﲪﻦ اﻟﺮﺣﲓ (( َوﯾَ ْﺴﺄَﻟُﻮﻧَ َﻚ َﻋ ِﻦ اﻟﺮﱡوحِ ﻗُ ِﻞ اﻟﺮﱡوحُ ِﻣ ْﻦ ُ أَ ْﻣ ِﺮ َرﺑﱢﻲ َو َﻣﺎ أوﺗِﯿﺘُ ْﻢ ِﻣ َﻦ ْاﻟ ِﻌ ْﻠ ِﻢ إِ ﱠﻻ ﻗَﻠِ ًﯿﻼ )). ﺳﻮرة اﻻٕﴎاء ا�ٓﯾﺔ (85) ﺻﺪق ﷲ اﻟﻌﻈﲓ DeDicatio To: My parents My brothers and sisters My wife My son (Mohamed) ACKNOWLEDGEMENT In the name of Allah, Almighty who taught man about matter that he does not know, prayers and peace be upon our Prophet Mohammed and his good followers till the Day of Judgment. I am most grateful to Allah, Almighty for helping and guiding me throughout my life. With respect and gratitude, I would like to extend my thanks to my supervisor Professor, Dr. Mohamed Abdel Karim Mohammed for his continuous encouragement, excellent supervision, valuable advice and constructive criticism that help me geting through many intricate phases during the preparation of this thesis. Thanks are also due to my co-supervisor Dr. Mohamed Al-Mubarak Osman for his help, support, advice and cooperation. My thanks are also extended to the technical staff of the faculty of science, Sudan University of science and technology for their support and help throughout this study.
    [Show full text]
  • Phytochemistry & Pharmacological Studies of Glycyrrhiza Glabra
    Int. J. Pharm. Sci. Rev. Res., 67(1), March - April 2021; Article No. 30, Pages: 187-194 ISSN 0976 – 044X Review Article Phytochemistry & Pharmacological Studies of Glycyrrhiza glabra: A Medicinal Plant Review Deeksha Sharma1, Priyanka Namdeo*2, Priti Singh3 1Assistant Professor, Department of Pharmacology, Mittal Institute of Pharmacy, Bhopal, M.P. INDIA. 2Associate Professor, Department of Pharmacology, Mittal Institute of Pharmacy, Bhopal, M.P. INDIA. 3Department of Pharmaceutics, Mittal Institute of Pharmacy, Bhopal, M.P. INDIA. *Corresponding author’s E-mail: [email protected] Received: 10-01-2021; Revised: 20-02-2021; Accepted: 28-02-2021; Published on: 20-03-2021. ABSTRACT Plants have been one of the important sources of medicines for human being and animals since the ancient time. Herbal medicines have attained popularity at global level to replace the synthetic chemicals as they have shown less adverse reactions. Glycyrrhiza glabra Linn is a herb belonging to the family Leguminosae, also known as Liquorice and sweetwood, is native to the Mediterranean and certain areas of Asia. Historically, the dried rhizome and root of this plant were employed medicinally by the Egyptian, Chinese, Greek, Indian and Roman civilizations as an expectorant and carminative. It is widely used in ayurvedic formulations and herb for different diseases. Present review article deals with chemical constituents present in various parts of Glycyrrhiza glabra and pharmacological activities. Present article object to comply all the updated information on its phytochemical and pharmacological activities which were performed by widely different methods. Glycyrrhiza glabra Linn possesses antibacterial, antioxidant, antimalarial, antispasmodic, anti-inflammatory and anti-hyper glycemic properties.
    [Show full text]