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MICROSCOPICAL and PHYSICO-CHEMICAL ASPECTS Are Rejected Scientific Works. Series C. Veterinary Medicine. Vol. LXIV (2), 2018 ISSN 2065-1295; ISSN 2343-9394 (CD-ROM); ISSN 2067-3663 (Online); ISSN-L 2065-1295 is excluded). Also, boars, pregnant sows or Sibiu” are similar in terms of formulation. animals fed with oleaginous plants or fish flour However, “Salam de Hateg” distinguishes by MICROSCOPICAL AND PHYSICO-CHEMICAL ASPECTS are rejected. In the production of the Sibiu the inclusion of beef mixed with pork and a OF THE COMPOSITION AND INTEGRITY OF RAW DRIED SALAMI salami are used only half-carcasses from pigs hydrolysed vegetable protein (Table 1). WITH NOBLE MOULD that weigh over 130 kg, belong to the Man- galita breed and are degreased and refrigerated Table 1. Label composition of analyzed samples Isabela Voichița ISACONI (BULAI)1, Ștefania RAITA1, Claudia Mariana CONSTANTINESCU1, for minimum 72 hours before processing. The Sibiu salami Sample A Sample B Sample C 1 1 pork, fat, salt, fat used must have increased consistency and pork, fat, salt, Teodoru SOARE , Manuella MILITARU sugars, spices, label sugars, spices, high freshness. The mixture of spices can be ascorbic acid, unknown recipe composition ascorbic acid, 1 brandy 0.4%, made according to different recipes or accor- sodium nitrite University of Agronomic Sciences and Veterinary Medicine of Bucharest, Faculty of Veterinary Medicine, sodium nitrite 105 Spaiul Independenței, District 5, Bucharest, Romania ding to the requirements of the beneficiary It Sibiu salami Sample D Sample E Sample F pork, fat, salt, can include the following: sodium chloride, pork, fat, salt, pork, fat, salt, dextrose, spices, glucono-lactone, carbohydrates, ascorbic acid/ sugars, spices, dextrose, ascorbic acid, label ascorbic acid, spices, sodium sodium Corresponding author email: [email protected] ascorbates, organic food acids (citric, lactic, composition sodium nitrite, ascorbate, ascorbate, tartaric), enibahar, garlic, sugar, and starter starter culture sodium nitrite sodium nitrite, Abstract cultures (Comanaru, 2000). starter culture Hațeg salami Sample G The quality of the Sibiu salami depends directly beef, pork, soybean protein, salt, sugar, spices and label Raw dried salamis with noble mould are among Romania’s most popular meat food products. The assumption that spice extracts, corn hydrolysed protein, sodium on the quality of raw material as it is raw dried composition there might be an unknown side to the structural integrity of the ingredients in such products has developed over time. ascorbate, carmine, sodium nitrite, starter culture A physico-chemical analysis was carried out on six salami samples of various brands of the raw dried salamis known salami that is processed only by cold smoking as “Salam de Sibiu” and “Salam de Hateg”, both available on the retail market. The following parameters were and maturing by drying, without the use of The following analyses were performed: mois- measured: humidity percentage, nitrites content, NaCl content, easily hydrolysable nitrogen, percentage of fat and thermal treatments. ture content, nitrites content, collagen/ protein amount of protein. All samples underwent the histopathologic examination routine procedure with paraffin and HE The histological method and physico-chemical ratio, NaCl content, easily hydrolysable (haematoxylin and eosin) stain. The values of the integrity parameters comply with the applicable legislation, but vary analysis are the mostly applied techniques for significantly from a producer to another. The histopathologic examination indicated the presence of animal tissue and nitrogen, lipids and proteins content. plant structures. We consider this examination to be useful in identifying structural non-compliance. detecting unauthorized tissues in meat pro- From each sample 200-300 g were taken ducts. Sezer et al. (2013) showed in their study according to protocol, from the middle and the Key words: raw dried salamis, physico-chemical analysis, histopathologic examination. that in a type of sausage they found animal hair ends of the bars (Purcărea, 2015). of root of hair, spleen, esophagus and epi- The samples were previously prepared accor- air and it is often mould-maturated. INTRODUCTION thelium of sensory organs, tissues that should ding to the methods that were going to be used Preparations that are typical for a region or area not exist in this food product (Sezer et al., and with the results that were going to be Salami is dried fermented sausage consisting of have specific characteristics derived from the 2013). In another study also using a histolo- obtained. pork mixed in different proportions with beef, use of ingredients and local production gical approach Malakauskienė et al. (2016) dis- The determination of moisture content was mutton or horse meat with different additives, techniques that are deeply rooted in tradition covered nerve tissue, fat and blood vessels in obtained by oven drying. represented by salt, spices and curing salt and linked to the territory where they come canned sausages (Malakauskienė et al., 2016). The determination of the nitrite content was (Feiner, 2006). from (Aquilati et al., 2016). Therefore, the physico-chemical and histolo- performed by Griess method according to the Salami was produced for the first time in Italy In Romania, among the most popular varieties gical evaluation of the composition of this type SR EN 12014-3:2005. more than 270 years ago (Leistner, 1986). of raw dried salami we mention: Banat salami, of meat preparation is essential for the quality For the determination of total nitrogen and total The European countries recognized for the Bacau salami, Sibiu salami and Hateg sausages assessment. content of protein the Kjeldahl method was production of salami are: Italy, Germany, (Mencinicopschi et al., 2006). The aim of the present paper is to evaluate the used, according to SR ISO 937: 2007. Spain, France and Hungary. They produce The Sibiu salami is a type of salami that is quality of the Sibiu Salami by analysing the The content of easily hydrolysable nitrogen several hundred million kilograms of salami made of raw meat. Due to the artificial climate regular physico-chemical parameters and by was indirectly determined by titration with per year (Bertolini et al., 2006). conditions this product can be manufactured in performing the histological analysis of the NaOH according to SR 9065-7: 2007. The suitable technology is selected according any part of the country throughout the year. composition in order to emphasise the parti- The content of lipids was determined by using to climatic conditions, as seen in the The materials used for the Sibiu salami are cularities derived from each method in terms of the Soxhlet method. Mediterranean region and Southern Europe, pork and pork fat. product integrity and composition assessment. The regulations for the minimum and maxi- where meat products are dried to lower the The pig has to be healthy, to have a reduced mum values of the parameters pursued in this amount of water taking advantage of long dry degree of contamination and to be properly MATERIALS AND METHODS study for the product groups mentioned above and sunny days, while in Northern Europe refrigerated. It must neither be too young, nor are found in The Order MARD 560 / fermented sausages require smoke for too fat. It needs to have a certain ratio of Physico-chemical analysis were carried out on 16.08.2006 and the means of conducting the subsequent preservation (Toldra, 2014). water/protein and fat/protein content, to be rich six salami samples of various brands of raw analyses are described in ISO Reference In Northern and Central Europe, smoked salami in heminic compounds (myoglobin), to have a dried salami known as “Salam de Sibiu” and Standards. In order to obtain high accuracy is preferred, and maturation is controlled by the low amount of connective tissue and to have an “Salam de Hateg”, both available in the retail results, the analyses were performed at a addition of acid-starter cultures, while in optimal water retention capacity (the PSE-pale, chain. The analysed samples of “Salam de laboratory accredited by RENAR. Southern Europe, salami is slowly dried in the soft, exsudative and DFD- dark, firm, dry meat 42 is excluded). Also, boars, pregnant sows or Sibiu” are similar in terms of formulation. animals fed with oleaginous plants or fish flour However, “Salam de Hateg” distinguishes by MICROSCOPICAL AND PHYSICO-CHEMICAL ASPECTS are rejected. In the production of the Sibiu the inclusion of beef mixed with pork and a OF THE COMPOSITION AND INTEGRITY OF RAW DRIED SALAMI salami are used only half-carcasses from pigs hydrolysed vegetable protein (Table 1). WITH NOBLE MOULD that weigh over 130 kg, belong to the Man- galita breed and are degreased and refrigerated Table 1. Label composition of analyzed samples Isabela Voichița ISACONI (BULAI)1, Ștefania RAITA1, Claudia Mariana CONSTANTINESCU1, for minimum 72 hours before processing. The Sibiu salami Sample A Sample B Sample C 1 1 pork, fat, salt, fat used must have increased consistency and pork, fat, salt, Teodoru SOARE , Manuella MILITARU sugars, spices, label sugars, spices, high freshness. The mixture of spices can be ascorbic acid, unknown recipe composition ascorbic acid, 1 brandy 0.4%, made according to different recipes or accor- sodium nitrite University of Agronomic Sciences and Veterinary Medicine of Bucharest, Faculty of Veterinary Medicine, sodium nitrite 105 Spaiul Independenței, District 5, Bucharest, Romania ding to the
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