APPENDIX 1 Final Report of Pilot Project: 1 April to 30 Nov 2004
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Phylogenetics of Tribe Orchideae (Orchidaceae: Orchidoideae)
Annals of Botany 110: 71–90, 2012 doi:10.1093/aob/mcs083, available online at www.aob.oxfordjournals.org Phylogenetics of tribe Orchideae (Orchidaceae: Orchidoideae) based on combined DNA matrices: inferences regarding timing of diversification and evolution of pollination syndromes Luis A. Inda1,*, Manuel Pimentel2 and Mark W. Chase3 1Escuela Polite´cnica Superior de Huesca, Universidad de Zaragoza, carretera de Cuarte sn. 22071 Huesca, Spain, 2Facultade de Ciencias, Universidade da Corun˜a, Campus da Zapateira sn. 15071 A Corun˜a, Spain and 3Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK * For correspondence. E-mail [email protected] Received: 3 November 2011 Returned for revision: 9 December 2011 Accepted: 1 March 2012 Published electronically: 25 April 2012 † Background and aims Tribe Orchideae (Orchidaceae: Orchidoideae) comprises around 62 mostly terrestrial genera, which are well represented in the Northern Temperate Zone and less frequently in tropical areas of both the Old and New Worlds. Phylogenetic relationships within this tribe have been studied previously using only nuclear ribosomal DNA (nuclear ribosomal internal transcribed spacer, nrITS). However, different parts of the phylogenetic tree in these analyses were weakly supported, and integrating information from different plant genomes is clearly necessary in orchids, where reticulate evolution events are putatively common. The aims of this study were to: (1) obtain a well-supported and dated phylogenetic hypothesis for tribe Orchideae, (ii) assess appropriateness of recent nomenclatural changes in this tribe in the last decade, (3) detect possible examples of reticulate evolution and (4) analyse in a temporal context evolutionary trends for subtribe Orchidinae with special emphasis on pollination systems. -
Orchis Militaris L
Orchis militaris L. Military Orchid Orchis militaris is one of four ‘anthropomorphic’ orchids found in England, with the flower structure said to resemble a soldier complete with helmet (converging sepals), arms (labellum lobes) and tunic resplendent with buttons (purple papillae on the labellum). It is a plant of unimproved grassland, scrub edge and woodland glades on free-draining calcareous soils and is restricted to a single site in Suffolk and two locations in Buckinghamshire, although it was formerly much more widely scattered across the Chilterns. The species has been assessed as Vulnerable in Great Britain and England due to the small number of extant locations and restricted population size. ©Pete Stroh IDENTIFICATION up to 1 4cm long, becoming more lax as flowering progresses and containing large, faintly scented pinkish-reddish purple Orchis militaris is the type species of the type genus of the flowers (Foley & Clarke, 2005; Stace, 2010). Each flower has orchid family. It has 3-6 oblong-lanceolate unspotted basal fiv e pointed sepals (outer perianth segments, each 10-15 mm) leaves 8-18 cm long and 2-5 cm wide that are shiny yellow that are a distinctive light ash-grey or pale rose-pink on the green on the upper side, paler beneath and with an obtuse to outside, whitish with purple lines on the inside. The sepals acute tip. Smaller sheathing leaves (1-4) also surround the conv erge to resemble a helmet. Sometimes called the ‘Soldier’ lower part of the stem. orchid, its anthropomorphic attributes are completed by a The inflorescence, borne on a stem 20-60 cm tall and often trilobed labellum which has short (up to 8 mm) lateral lobes v iolet-tinged, is at first a fairly dense ovoid or cylindrical spike (‘arms’), a longer bilobed middle lobe (legs) with a short tooth between the lobes, and purple papillae that are said to look like the buttons on a soldier’s tunic, although others see more of a resemblance with baggy pink pyjamas (Brooke & Bone, 1 950). -
Distribution and Conservation Status of Some Rare and Threatened Orchid
Wulfenia 24 (2017): 143 –162 Mitteilungen des Kärntner Botanikzentrums Klagenfurt Distribution and conservation status of some rare and threatened orchid taxa in the central Balkans and the southern part of the Pannonian Plain Vladan Djordjević, Dmitar Lakušić, Slobodan Jovanović & Vladimir Stevanović Summary: Along with being a centre of plant species diversity and endemism, the Balkan Peninsula is one of the parts of Europe with the highest number of orchid taxa. However, the orchid flora in the central Balkans has not been sufficiently studied. The paper presents the distribution of ten rare and threatened taxa of Orchidaceae in the central Balkans and the southern part of the Pannonian Plain: Anacamptis papilionacea, Epipactis palustris, E. purpurata, Epipogium aphyllum, Goodyera repens, Gymnadenia frivaldii, Ophrys apifera, O. insectifera, Orchis militaris and O. spitzelii subsp. spitzelii. In addition to field investigation, checking and revision of herbarium material, literature sources were also used for supplementing distribution data. The distribution maps of these taxa in the central Balkans (Serbia and Kosovo region) and the southern part of the Pannonian Plain (Vojvodina) are created on a 10 km × 10 km UTM grid system. Data concerning their habitat preferences, population size and the estimated IUCN conservation status in the study area are provided. Keywords: Orchidaceae, phytogeography, IUCN conservation status, Balkan Peninsula The orchid family is one of the largest and most diverse families in the plant kingdom with estimates of about 28 000 species distributed in about 763 genera (Chase et al. 2015; Christenhusz & Byng 2016). According to Hágsater & Dumont (1996), over 300 orchid species occur in Europe, North Africa and Near East. -
ORCHID CONSERVATION NEWS the Newsletter of the Orchid Specialist Group of the IUCN Species Survival Commission
ORCHID CONSERVATION NEWS The Newsletter of the Orchid Specialist Group of the IUCN Species Survival Commission Issue 1 March 2021 PATHS TOWARD CONSERVATION PROGRESS Orchid workshop at Bogotá Botanic Garden, Colombia in 2017 1 https://www.bgci.org/our-work/plant- Editorial conservation/conservation-prioritisation/ex-situ- At the time of this first Issue of 2021, many challenges surveys/ still lie before us, lots of unknowns yet to be determined with the pandemic at the forefront of our thoughts. We Why am I puzzled? Well firstly, I don’t know where are doing our best to continue our conservation work the figure of 38% has come from. Although encouraging despite constraints whether it be project planning, data progress is being made with Red Listing, I don’t think collection and management, seed banking, evaluating we know how many species are threatened globally. conservation strategies, or continuing studies of orchid Secondly, does just one individual plant count as an ex populations over the long term. With the situ collection? Surely we need to be focusing on unpredictability and randomness of natural events that conserving as far as possible the genetic diversity within may threaten orchid ecosystems, long-term monitoring each species. Thirdly, the table doesn’t tell me whether studies are being re-visited years, even decades after the collection is plants and/or seed. their initiation, to study what has been happening following severe disturbance. For example, Deschênes, The BGCI report asserts that botanical gardens are the Brice & Brisson (2019) have reported, after an initial main repository of orchid collections. -
Ophrys Apifera Huds. (Orchidaceae) on a Heap of Limestone Mine Waste – the First Population Found in the Sudetes and the Second in Poland
Biodiv. Res. Conserv. 59: 9-14, 2020 BRC www.brc.amu.edu.pl Doi 10.2478/biorc-2020-0007 Submitted 05.08.2020, Accepted 14.09.2020 Ophrys apifera huds. (orchidaceae) on a heap of limestone mine waste – the first population found in the Sudetes and the second in Poland Anna Wójcicka-Rosińska1, Dariusz Rosiński1 & ewa Szczęśniak2* 1geosilva Sp. z o.o., J. Tuwima 11, 54-200 oława, Poland 2Department of Botany, institute of environmental Biology, University of Wrocław, h. Sienkiewicza 21, 50-335 Wrocław, Poland; oRCiD 0000-0002-8162-7520 * corresponding author (e-mail: [email protected]) Abstract. The paper reports the finding of a new locality of Ophrys apifera – the first locality of this species in the Sudeten range and the second in Poland. A new population was found in the Kaczawskie Mts. (Western Sudetes). it consists of 23 flowering plants and occurs in an operating limestone quarry. The detailed data on this population and its habitat are included. Key words: Ophrys apifera, bee-orchid, orchids, distribution changes, Sudetes 1. Introduction europe, Middle east up to Caucasus and Turkmeni- stan), but mostly occurrs in the Mediterranean region. The genus Ophrys L. is known to botanists and Areas of high diversity comprise southern italy and orchid enthusiasts as a model system of floral evolu- the Aegean region (Soliva et al. 2001); the number of tion and pollination mimicry. These orchids developed Ophrys species decreases to the north and north-east a specialized flower-pollinator relationship of pollina- of europe. in Central europe, up to the end of the 20th tion by deception, more precisely – sexual deception century, only a few species were noted: 4 in germany (pseudo copulation; Schiestl 2005). -
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Firenze University Press Caryologia www.fupress.com/caryologia International Journal of Cytology, Cytosystematics and Cytogenetics Comparison of the Evolution of Orchids with that of Bats Citation: A. Lima-de-Faria (2020) Comparison of the Evolution of Orchids with that of Bats. Caryologia 73(2): 51-61. doi: 10.13128/caryologia-891 Antonio Lima-de-Faria Received: February 12, 2020 Professor Emeritus of Molecular Cytogenetics, Lund University, Lund, Sweden E-mail: [email protected] Accepted: April 16, 2020 Published: July 31, 2020 Abstract. The evolution of orchids and bats is an example of DNA’s own evolution which has resulted in structures and functions which are not necessarily related to any Copyright: © 2020 A. Lima-de-Faria. obvious advantage to the organism. The flowers of orchids resemble: humans, apes, liz- This is an open access, peer-reviewed article published by Firenze University ards, frogs and even shoes. The faces of bats resemble plant leaves but also horseshoes. Press (http://www.fupress.com/caryo- These similarities are not accidental because they emerge repeatedly in different gen- logia) and distributed under the terms era and different families. This evolutionary situation bewildered botanists and zoolo- of the Creative Commons Attribution gists for many years, but is now elucidated by the molecular unification of plants and License, which permits unrestricted animals derived from the following evidence: (1) Contrary to expectation, plant and use, distribution, and reproduction animal cells (including those of humans) could be fused and the human chromosomes in any medium, provided the original were seen dividing in the plant cytoplasm. (2) Orchids, bats and humans have about author and source are credited. -
Insect Visitors and Potential Pollinators of Orchis Militaris (Orchidaceae) in Southern Belgium
Journal of Insect Science (2016) 16(1): 104; 1–7 doi: 10.1093/jisesa/iew088 Short Communication Insect Visitors and Potential Pollinators of Orchis militaris (Orchidaceae) in Southern Belgium Thomas Henneresse,1,2 and Daniel Tyteca1 1Biodiversity Research Centre, Earth and Life Institute, Universite´ catholique de Louvain, Croix du Sud 4-5 box L7.07.04, B-1348, Louvain-la-Neuve, and 2Corresponding author, e-mail: [email protected] Subject Editor: Dr. Hongmei Li-Byarlay Received 14 June 2016; Accepted 24 August 2016 Abstract As part of a research project on the food deception strategy in Orchis militaris (L.), the objective of this study was to identify insect visitors and potential pollinators of this orchid species in Belgium. In 2013, insects were collected over 2 d per site in five localities distributed in Southern Belgium (Wallonia). A total of 104 insects be- longing to 49 species were caught. Dipterans were the most abundant visitors (50% of total specimens), fol- lowed by Hymenopterans (32%). Rhingia campestris Meigen, Bombylius venosus Mikan, Apis mellifera (L.), and Bombus lapidarius (L.) were the most abundant species. Only five specimens bore one to more than 10 pol- linia: four honeybees (A. mellifera) and one bumblebee worker (B. lapidarius). These two species should be con- sidered as potential pollinators in the study area, but probably not confirmed ones. Key words: Orchis militaris, pollinator, Apidae, Apis mellifera, Bombus lapidarius Orchidaceae are one of the most diversified Angiosperm families, accurate identification of numerous insects (especially dipterans and with more than 27,000 accepted names (The Plant List 2013). -
Ancestral State Reconstruction of the Mycorrhizal Association for the Last Common Ancestor of Embryophyta, Given the Different Phylogenetic Constraints
Supplementary information Supplementary Figures Figure S1 | Ancestral state reconstruction of the mycorrhizal association for the last common ancestor of Embryophyta, given the different phylogenetic constraints. Pie charts show the likelihood of the ancestral states for the MRCA of Embryophyta for each phylogenetic hypothesis shown below. Letters represent mycorrhizal associations: (A) Ascomycota; (B) Basidiomycota; (G) Glomeromycotina; (M) Mucoromycotina; (-) Non-mycorrhizal. Combinations of letters represent a combination of mycorrhizal associations. Austrocedrus chilensis Chamaecyparis obtusa Sequoiadendron giganteum Prumnopitys taxifolia Prumnopitys Prumnopitys montana Prumnopitys Prumnopitys ferruginea Prumnopitys Araucaria angustifolia Araucaria Dacrycarpus dacrydioides Dacrycarpus Taxus baccata Podocarpus oleifolius Podocarpus Afrocarpus falcatus Afrocarpus Ephedra fragilis Nymphaea alba Nymphaea Gnetum gnemon Abies alba Abies balsamea Austrobaileya scandens Austrobaileya Abies nordmanniana Thalictrum minus Thalictrum Abies homolepis Caltha palustris Caltha Abies magnifica ia repens Ranunculus Abies religiosa Ranunculus montanus Ranunculus Clematis vitalba Clematis Keteleeria davidiana Anemone patens Anemone Tsuga canadensis Vitis vinifera Vitis Tsuga mertensiana Saxifraga oppositifolia Saxifraga Larix decidua Hypericum maculatum Hypericum Larix gmelinii Phyllanthus calycinus Phyllanthus Larix kaempferi Hieronyma oblonga Hieronyma Pseudotsuga menziesii Salix reinii Salix Picea abies Salix polaris Salix Picea crassifolia Salix herbacea -
DNA Barcoding of Native Caucasus Herbal Plants: Potentials and Limitations in Complex Groups and Implications for Phylogeographic Patterns
Biodiversity Data Journal 9: e61333 doi: 10.3897/BDJ.9.e61333 Research Article DNA barcoding of native Caucasus herbal plants: potentials and limitations in complex groups and implications for phylogeographic patterns Parvin Aghayeva‡, Salvatore Cozzolino§, Donata Cafasso§‡, Valida Ali-zade , Silvia Fineschi|, Dilzara Aghayeva‡ ‡ Institute of Botany, Azerbaijan National Academy of Sciences, Baku, Azerbaijan § Department of Biology, University of Naples Federico II, Complesso Universitario di Monte S. Angelo, Napoli, Italy | CNR - Istituto di Scienze del Patrimonio Culturale, Sesto Fiorentino, Italy Corresponding author: Dilzara Aghayeva ([email protected]) Academic editor: Anatoliy Khapugin Received: 24 Nov 2020 | Accepted: 07 Jan 2021 | Published: 27 Jan 2021 Citation: Aghayeva P, Cozzolino S, Cafasso D, Ali-zade V, Fineschi S, Aghayeva D (2021) DNA barcoding of native Caucasus herbal plants: potentials and limitations in complex groups and implications for phylogeographic patterns. Biodiversity Data Journal 9: e61333. https://doi.org/10.3897/BDJ.9.e61333 Abstract DNA barcoding has rapidly become a useful complementary tool in floristic investigations particularly for identifying specimens that lack diagnostic characters. Here, we assess the capability of three DNA barcode markers (chloroplast rpoB, accD and nuclear ITS) for correct species assignment in a floristic survey on the Caucasus. We focused on two herbal groups with potential for ornamental applications, namely orchids and asterids. On these two plant groups, we tested whether our selection of barcode markers allows identification of the “barcoding gap” in sequence identity and to distinguish between monophyletic species when employing distance-based methods. All markers successfully amplified most specimens, but we found that the rate of species-level resolution amongst selected markers largely varied in the two plant groups. -
The Orchidaceae Is the Largest of the Flowering Plant
TABLE OF CONTENTS INTRODUCTION pg. 1 ORCHID SYSTEMATIC pg. 3 ORCHID FLORAL MORPHOLOGY pg. 9 ORCHID AERAL PARTS pg. 12 ORCHID UNDERGROUND PARTS pg. 12 ORCHID REPRODUCTION AND LIFE CYCLE pg. 14 POLLINATION pg. 16 ORCHID HYBRIDIZATION AND REPRODUCTIVE BARRIERS pg. 20 ORCHID MYCORRHIZAE pg. 23 PURPOSE pg. 26 Orchis xcolemanii hybridization: Molecular and morphological evidence, seed set success, and evolutionary importance. Introduction pg. 27 Materials and methods pg. 31 - Study area and orchid species studied pg. 31 - Phenotipic trait measurements pg. 36 - Molecular analysis pg. 36 - Pollen transfer pg. 39 - Reproductive success and hand pollination pg. 39 Results pg. 41 - Orchid xcolemanii survey pg. 41 - Phenotipic trait measurements pg. 41 - Molecular analysis pg. 44 - Pollen transfer pg. 45 - Reproductive success pg. 46 Discussion pg. 48 i 2-Interactions with symbionts in a hybrid Mediterranean orchid. Introduction pg. 56 Materials and methods pg. 60 - Study area and orchid species studied pg. 60 - Molecular analysis pg. 64 Results pg. 66 Discussion pg. 69 3-Pollen competition as a reproductive isolating mechanism between two sympatric Orchis species. Introduction pg. 71 Materials and methods pg. 74 - Study area and orchid species studied pg. 74 - Pollination experiments pg. 75 - Molecular analysis pg. 77 Results pg. 80 Discussion pg. 85 REFERENCES pg. 88 ii Ai miei adorati nipoti INTRODUCTION The Orchidaceae is one of the largest plant families on Earth, including almost 10% (approximately 20,000 species) of all flowering plant species (Dressler 1993; Dixon et al., 2003); It is rivalled only by the Asteraceae, which contains approximately 23 000 species (Bremer, 1994). Orchids show a wide diversity of epiphytic and terrestrial growth forms and have successfully colonized almost every habitat on earth. -
Orchids of the Black Sea Coast of Krasnodarsky Krai (Russia): Current State, New Records, Conservation
Nature Conservation Research. Заповедная наука 2020. 5(Suppl.1): 46–68 https://dx.doi.org/10.24189/ncr.2020.047 ORCHIDS OF THE BLACK SEA COAST OF KRASNODARSKY KRAI (RUSSIA): CURRENT STATE, NEW RECORDS, CONSERVATION Anton V. Popovich1, Elena A. Averyanova2, Lev M. Shagarov2,3 1Russian Geographical Society, Russia e-mail: [email protected] 2RUDN University, Russia e-mail: [email protected] 3Institute of Natural and Technical Systems, Russia e-mail: [email protected] Received: 09.05.2020. Revised: 12.07.2020. Accepted: 11.08.2020. The article provides a relevant nomenclature list of Orchidaceae on the Black Sea coast in Krasnodarsky Krai (Russia), with clarification of the taxonomic status of each taxon. Fifty-one taxa (41 species and eleven subspecies) are known in the study area; apart of them, there are ten hybrids. Of them, five taxa are not found anywhere else in Russia, including Cephalanthera epipactoides, Epipactis euxina (endemic), E. leptochila subsp. neglecta, E. pontica, and Serapias orientalis subsp. feldwegiana. Two taxa (Epipactis condensata and Ophrys mammosa subsp. caucasica) slightly exceed the boundaries of the study area. Data on the distribution, occurrence, and association of species with plant communities are provided. Most taxa are more or less represented in forest communities. Only eight species are confined to grassland communities and ecotonic habitats. For 19 taxa, favourable conditions seem to be in anthropogenically disturbed habitats, preferring clearings under power lines, sides of forest roads, deposits, hay meadows, and dendroparks. Data on new locations and the state of the most threatened orchid species on the Black Sea coast in Krasnodarsky Krai are presented. -
Salep” in the Bulgarian Rhodopes Hazardous for the Wild Populations of Terrestrial Orchids?
Fl. Medit. 28: 399-418 doi: 10.7320/FlMedit28.399 Version of Record published online on 20 December 2018 I. Mincheva, A. Petrova, M. Yordanova & E. Kozuharova Is the traditional use of “salep” in the Bulgarian Rhodopes hazardous for the wild populations of terrestrial orchids? Abstract Mincheva, I., Petrova, A., Yordanova, M. & Kozuharova, E.: Is the traditional use of “salep” in the Bulgarian Rhodopes hazardous for the wild populations of terrestrial orchids? — Fl. Medit. 28: 399-418. 2018. — ISSN: 1120-4052 printed, 2240-4538 online. In Bulgaria and the eastern Mediterranean, dried tubers of terrestrial orchids are known as “salep”, which also refers to ground tuber powder and the beverage made from this powder. Collection of “salep” from wild populations was shown to be hazardous factor for the native populations. In this preliminary study we investigate how popular “salep” is in the Bulgarian Rhodopes and to what extent it is collected and utilized. Distribution maps were constructed based on personal data, data from Herbaria, and publications from the Bulgarian part of the Rhodopes. During 2014 and 2015 we performed semi-structured interviews in different loca- tions in the Rhodopes in order to evaluate the popularity, use and collection of “salep”. The ini- tial data obtained from the interviews indicate that “salep” is not popular amongst the local peo- ple in the Rhodopes and especially Central Rhodopes. Most informants responded that “they have never heard of “salep”. Some informants responded that they know the name but details about use are blurring. For the moment the local human activity does not threaten the wild orchid populations, but permanent monitoring in necessary.