bioRxiv preprint doi: https://doi.org/10.1101/2020.01.16.910000; this version posted January 17, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Two pectate lyases from Caldicellulosiruptor bescii with the same CALG domain had 2 distinct properties on plant biomass degradation 3 Hamed I. Hamoudaa,b,c, Nasir Alia, Hang Sua,b, Jie Fenga, Ming Lua,†and Fu-Li Li a,† 4 a Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuel, 5 Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 6 Qingdao 266101, China 7 b University of Chinese Academy of Sciences, Beijing 100039, China. 8 c Egyptian Petroleum Research Institute, Nasr City 11727, Cairo, Egypt. 9 †Corresponding authors: Dr. Ming Lu (E-mail:
[email protected]) and Dr. Fu-Li Li 10 (E-mail:
[email protected]), Qingdao Institute of Bioenergy and Bioprocess Technology, 11 Chinese Academy of Sciences, Qingdao 266101, China 12 13 Keywords: Caldicellulosiruptor, Pectin, Pectate lyase, Polysaccharide lyase, Concanavalin 14 A-like lectin/glucanase (CALG) 15 16 17 18 19 20 21 22 23 24 25 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.01.16.910000; this version posted January 17, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 26 Abstract 27 Pectin deconstruction is the initial step in breaking the recalcitrance of plant biomass by using 28 selected microorganisms that carry pectinolytic enzymes.