RESEARCH ARTICLE Age, gender and UV-exposition related effects on gene expression in in vivo aged short term cultivated human dermal fibroblasts Wolfgang Kaisers1¤*, Petra Boukamp4,5, Hans-JuÈ rgen Stark5, Holger Schwender1,2, Julia Tigges4, Jean Krutmann4,6, Heiner Schaal3 1 Center for Bioinformatics and Biostatistics, BMFZ, Heinrich-Heine-UniversitaÈt DuÈsseldorf, DuÈsseldorf, Germany, 2 Mathematical Institute, Heinrich-Heine-UniversitaÈt DuÈsseldorf, DuÈsseldorf, Germany, 3 Institut fuÈr Virologie, Heinrich-Heine-UniversitaÈt DuÈsseldorf, DuÈsseldorf, Germany, 4 IUF - Leibniz Research Institute for a1111111111 Environmental Medicine, DuÈsseldorf, Germany, 5 German Cancer Research Center (DKFZ), Heidelberg, a1111111111 Germany, 6 Medical Faculty, Heinrich-Heine-UniversitaÈt DuÈsseldorf, DuÈsseldorf, Germany a1111111111 a1111111111 ¤ Current address: Department of Anaesthesiology, Heinrich-Heine-UniversitaÈt DuÈsseldorf, DuÈsseldorf, a1111111111 Germany *
[email protected] Abstract OPEN ACCESS Ageing, the progressive functional decline of virtually all tissues, affects numerous living Citation: Kaisers W, Boukamp P, Stark H-J, Schwender H, Tigges J, Krutmann J, et al. (2017) organisms. Main phenotypic alterations of human skin during the ageing process include Age, gender and UV-exposition related effects on reduced skin thickness and elasticity which are related to extracellular matrix proteins. Der- gene expression in in vivo aged short term mal fibroblasts, the main source of extracellular fibrillar proteins, exhibit complex alterations cultivated human dermal fibroblasts. PLoS ONE 12(5): e0175657. https://doi.org/10.1371/journal. during in vivo ageing and any of these are likely to be accompanied or caused by changes in pone.0175657 gene expression. We investigated gene expression of short term cultivated in vivo aged Editor: Johnson Rajasingh, University of Kansas human dermal fibroblasts using RNA-seq.