Progress Report
2008–2009 We owe special thanks to the Austrian Science Fund (FWF) for its financial support for numerous projects of the research facilities of the Austrian Academy of Sciences
All rights reserved Copyright © 2009 by Austrian Academy of Sciences Layout: Art Quarterly Publishing House Werbe- und PR-Agentur GmbH. Printed and bound: Wograndl 3
Table of contents Preface ...... 5 RESEARCH FACILITIES OF THE SECTION FOR MATHEMATICS AND NATURAL SCIENCES Biology and Medicine CeMM – Research Center for Molecular Medicine GmbH ...... 11 Breath Research Institute ...... 14 GMI – Gregor Mendel Institute of Molecular Plant Biology ...... 18 IMBA – Institute of Molecular Biotechnology GmbH ...... 22 Institute for Biomedical Aging Research ...... 26 Institute for Biophysics and Nanosystems Research ...... 30 Konrad Lorenz Institute for Ethology ...... 34 Earth Sciences Institute for Geographic Information Science ...... 37 Center for Geosciences ...... 40 Commission for the Palaeontological and Stratigraphical Research of Austria ...... 42 Commission for Geophysical Research ...... 45 Commission for Quaternary Research ...... 48 Commission for Basic Research on Mineral Raw Materials ...... 51 Mathematics, Simulation and Metrology Institute for Integrated Sensor Systems ...... 55 Acoustics Research Institute ...... 58 Johann Radon Institute for Computational and Applied Mathematics ...... 61 Commission for Scientific Visualization ...... 65 Physics and Materials Sciences Erich Schmid Institute of Materials Science ...... 68 Institute of High Energy Physics ...... 71 Institute for Quantum Optics and Quantum Information ...... 74 Stefan Meyer Institute for Subatomic Physic...... 77 Environmental Research Institute for Limnology ...... 80 Institute of Technology Assessment ...... 83 Commission for Interdisciplinary Ecological Studies ...... 86 Space Research Space Research Institute ...... 89 Commission for Astronomy ...... 92 Interdepartmental Research Tasks Commission for Scientific Co-operation with the Austrian Federal Ministry of Defence and Sports ...... 94 Commission for the History of Natural Sciences, Mathematics and Medicine ...... 96 RESEARCH FACILITIES OF THE SECTION FOR HUMANITIES AND SOCIAL SCIENCES Centre for Ancient World Studies (CAWS) Commission for Egypt and the Levant ...... 101 Commission for Ancient Literature and Latin Tradition ...... 104 Commission for the History of Ancient Law ...... 107 Commission for Editing the Corpus of the Latin Church Fathers ...... 110 Asia Minor Commission ...... 113 Institute for the Studies of Ancient Culture ...... 116 Commission for Mycenaean Studies ...... 119 Numismatic Commission ...... 122 Prehistoric Commission ...... 125 Centre for Studies in Asian Cultures and Social Anthropology (CSACSA) Institute for Iranian Studies ...... 129 4 Table of contents
Institute for the Cultural and Intellectual History of Asia ...... 132 Institute for Social Anthropology ...... 135 Centre for Cultural Research (CCR) AAC – Austrian Academy Corpus ...... 138 Commission for the Edition of a Text Dictionary of „Die Fackel“ (Fackellex) ...... 141 Institute of Culture Studies and History of the Theatre ...... 143 Commission for the History of Art ...... 145 Commission for Music Research ...... 148 Centre for Medieval Studies (CMS) Institute for Byzantine Studies ...... 151 Institute for Medieval Research ...... 154 Institute for Medieval and Early Modern Material Culture ...... 157 Commission of Paleography and Codicology of Medieval Manuscripts in Austria ...... 161 Centre for Research on Modern and Contemporary History (CMC) Commission for the History of the Habsburg Monarchy ...... 164 Historical Commission ...... 168 Austrian Biographical Encyclopaedia and Biographical Documentation ...... 171 Commission for Austrian Legal History ...... 174 Social Sciences Research Centre (SSRC) Vienna Institute of Demography ...... 177 Institute for European Integration Research ...... 180 Institute for European Tort Law ...... 183 Institute for Mountain Research: Man and Environment ...... 185 Commission for Migration and Integration Research ...... 188 Institute for Urban and Regional Research ...... 190 Commission for Comparative Media and Communication Studies ...... 192 Centre for Linguistics and Audiovisual Documentation (LAVD) Commission for Balkan-Studies ...... 195 Commission of Linguistics and Communication Research ...... 198 Institute of Lexicography of Austrian Dialects and Names ...... 201 Phonogrammarchiv ...... 204 CENTRAL ADMINISTRATION UNITS Presidential Office ...... 211 Public Relations ...... 213 International Relations ...... 215 Information Management ...... 217 Fellowships and Awards ...... 218 Facility Management ...... 220 Event Management ...... 222 Office of the Secretary General ...... 224 Office of the Section for the Humanities and Social Sciences ...... 229 Accounting Office ...... 227 Human Resources ...... 228 Office for Legal Affairs ...... 230 Office of the Section for Mathematics and Natural Sciences ...... 231 Subsidiaries ...... 232 Information Technology Services (ITS) ...... 233 Internal Audit ...... 235 Akademie-Gebäude-Errichtungs- und Instandhaltungs-GmbH ...... 236 Library and Archive ...... 237 National and International Research Programmes ...... 240 Austrian Academy of Sciences Press and Repository ...... 244 5
Preface
The Austrian Academy of Sciences (OeAW) is the represented at the universities, long-term projects, a leading autonomous non-university research perfor- strong involvement in international major research and ming organisation in Austria. Its research units apply the strengthening of expertise already present in the the highest scientific quality standards to their activi- country. A new form of basic research unit is that adop- ties in the field of innovatory application-open basic ted by those institutes that are limited liability compa- research. The Academy provides an impetus by pursu- nies. They are more independent and at the same time ing new research directions, taking up research specia- can cooperate with business more easily. However, they lisations that cannot be pursued by universities and other also bear a greater degree of individual responsibility. research institutions, consolidating its existing scien- tific strengths, undertaking even risky research pro- The merger of smaller units to create scientific centres jects, exercising highly specialised research functions with scientific advisory boards has largely proved to be to maintain the cultural heritage and contributing to advantageous, as demonstrated by numerous projects, scientific careers by providing top quality training conferences and publications by the centres during the posts for young researchers. In addition, the Academy period under review. The first evaluations of various encourages young scientists in many respects, inclu- research units carried out under the auspices of the ding by awarding scholarships. Research Board of Trustees have been commenced.
The Academy fulfils additional functions by dissemina- The reorganisation of the Academy that entered into ting scientific knowledge to the public, by representing effect in 2008 was the beginning of a comprehensive the interests of science to society, by issuing comments structural reform that was adopted by the General Mee- on questions concerning science, research and research ting in June 2010 and is now being implemented. With policies and by preparing scientific expertises. these measures, the Academy is reacting to the huge in- crease in its duties in the last few years within its various The Academy’s research units map a wide range of the research-promoting functions. research landscape in Austria. They comprise technical sciences, biology, medicine and environment, physics, The present Progress Report for 2008 and 2009 space research, earth sciences, mathematics, social sci- presents an insight into the variety of the Academy’s ences, linguistics and literature studies, cultural studies, research and publication activities and is also historical studies and law. However, as already empha- intended to serve the public as evidence of the sised in the preceding progress report, it is not possi- Academy’s accomplishments. ble for the Academy of Sciences to fully cover all the sub-disciplines of science. Accordingly, additional criteria play an important role when a unit is to Prof. Dr. Helmut Denk Prof. Dr. Sigrid Jalkotzy-Deger be set up, such as complementarity to the subjects President Vice President 6 7
The Austrian Academy of Sciences is a learned society, next generation of researchers. The institutes belonging a research-performing organisation, an institution that to the Academy are undergoing a process of transfor- promotes young researchers and a purveyor of know- mation, a process which will have an effect on everyone ledge. As a research-performing organisation it car- participating in the research and innovations system, ries out basic research at the highest level. Through its and which will increase both their level of achievement scholarship programme the OeAW makes a sustainable and their capacity for networking. contribution to the career development of young resear- chers. As a purveyor of knowledge it is a hub for the The reinforcement of excellent research driven by a desi- exchange and discussion of scientific knowledge. re to increase knowledge should result in long-term, sustainable economic growth and have a positive The Activity Report for 2008 and 2009 provides an in- effect on the national economy as a whole. Furthermore, sight into the many activities and achievements of the it will contribute to the development of our culture in Academy and provides public evidence of the success general. Progress made in scientific research has a value enjoyed by the OeAW. 2006 saw the start of a reform of of its own, since by increasing reflective capabilities it the Austrian Academy of Sciences, the aim of which is to strengthens society’s cultural development. enable it to better fulfil its role as a research-performing organisation in the future. I wish the Academy and its employees the same success in future years as in the past two. The scientific system constitutes the most important node in the network of innovation, because it organises Dr. Beatrix Karl both the creation of knowledge and the training of the Federal Minister of Science and Research
Research Facilities of the Section for Mathematics and Natural Sciences
11
Biology and Medicine
CeMM – Research Center for Molecular Medicine GmbH
Head: Giulio Superti-Furga
Aims and Functions innovative diagnostic and therapeutic approaches. CeMM, the Research Center for Molecular Medicine These systems-biology techniques encompass modern of the Austrian Academy of Sciences, uses post-geno- post-genomic methods including proteomics, chemi- mic molecular technologies and systems-level approa- cal biology, biological-chip technologies and bioinfor- ches intersected with medical and clinical needs to matics. CeMM provides access to these technologies identify novel therapeutic and diagnostic approaches. to the biomedical community via research coopera- At the core of CeMM’s mission is the drive towards a tion and is a training and teaching center for a new more personalized, targeted and safe medicine. generation of researchers in molecular medicine.
As knowledge of human and biological processes is Results for 2008–2009 expanding exponentially, and while the scope for me- At the beginning of 2005, Giulio Superti-Furga took dical breakthroughs is potentially enormous, the costs over the scientific leadership of CeMM. Besides the in terms of developing clinical treatments can also Director’s group, CeMM consists of 5 Principal In- be prohibitive and this poses difficult moral issues vestigators, namely Denise Barlow, Christoph Bin- for society. The challenge is to produce better treat- der, Sylvia Knapp, Robert Kralovics and Sebastian ments that are both effective and sufficiently econo- Nijman. One of the organizational milestones in mically viable such that they can be made available to 2009 was the successful international recruitment of everyone. Though this is beyond the scope of any two additional PIs to complement and strengthen the single research institute, CeMM was founded by the current faculty. Kaan Boztug, MD/geneticist, wor- Austrian Academy of Sciences in an attempt to ac- king on innate deficiencies of the immune system, celerate this process and, together with the Medical and Olaf Gross, PhD, working on innate immunity University of Vienna (MUV) and the General Hospital from a cell- and systems-biology point of view, will of Vienna (AKH), to play an active and major role in both start at CeMM at the beginning of 2011. the Austrian biomedical research area. To better serve its mission, CeMM is constructing a new purpose-built In the last two years, CeMM members have been research centre located in the heart of the AKH cam- very successful, receiving many third-party funds and pus. The close cooperation with the Medical University awards. A short overview in chronological order: and the AKH offers a unique opportunity to contribu- t Robert Kralovics won the New Investigator Grant te to significant research findings. When scientists and of the American MPD (Myeloproliferative Disor- medical doctors work hand-in-hand, the clinical chal- ders) Foundation lenges can be solved and the vision of patient-oriented t Order of Merit of the Republic of Italy, Knight medicine can be achieved. Officer, awarded to Giulio Superti-Furga in recognition of his achievements as outstanding The research interests of CeMM focus on cancer, representative in the scientific and academic inflammation and immune disorders. At CeMM, the field of Austria mode-of-action of pathologically-relevant proteins t Jacques Colinge, Head of Bioinformatics, is sub- and novel or existing drugs is determined. Pathologica l project leader in the GEN-AU project BIN III processes are linked via an interdisciplinary systems- (Bioinformatics Integration Networks) coordina- biology strategy and implemented into alternative and ted by the Graz University of Technology 12 CeMM – Research Center for Molecular Medicine GmbH t Giulio Superti-Furga and Keiryn Bennett, Head Clinical Chemistry Laboratory, Geneva University of Mass Spectrometry, are subproject leaders in the Hospital, CH) GEN-AU project APP III (Austrian Proteomics t Prof. Dr. David Livingston (Deputy Director, Da- Platform) coordinated by the Medical University of na-Farber/Harvard Cancer Center, Boston, USA), Innsbruck chair of the SAB t Giulio Superti-Furga is coordinator of the t Prof. Dr. William E. Paul (Chief, Laboratory of GEN-AU project PLACEBO (Platform Austria for Immunology, National Institute of Allergy and Chemical Biology) Infectious Diseases, Bethesda, USA) t Christoph Binder appointed as Junior Profes- t Prof. Dr. Hidde Ploegh (Member, Whitehead Ins- sor for Atherosclerosis Research at the Medical titute for Biomedical Research, Cambridge, USA) University of Vienna t Prof. Dr. Nadia Rosenthal (Head, EMBL-Euro- t Willhelm-Türk-Award of the Austrian Society for He- pean Molecular Biology Laboratory, Monteroton- matology and Oncology awarded to Damla Olcaydu, do Outstation, Rome, I) Medical Doctor and Pre Doctoral Fellow at CeMM t Prof. Dr. Louis M. Staudt (Head, Molecular Bio- for her publication “A common JAK2 haplotype con- logy of Lymphoid Malignancies Section, National fers susceptibility to myeloproliferative neoplasms” Institutes of Health, National Cancer Institute, t Sebastian Nijman won a grant financed by the Bethesda, USA) Vienna Science and Technology Fund (WWTF) t Dame Prof. Dr. Janet Thornton (Director, and a second one funded by the Austrian Science European Bioinformatics Institute in Cambridge, Fund (FWF) on his research topic “Searching for UK, Group Leader, EMBL-European Molecu- Cancer’s Achilles’ Heels” lar Biology Laboratory, Hinxton Outstation, t “Wiener Zukunftspreis 2009” of the City of Vien- Cambridge, UK). na awarded to Sebastian Nijman in the category “Newcomer & Start-ups” Bibliography t Giulio Superti-Furga was awarded the Karl In 2008 and 2009, we have seen the fruits of the first Landsteiner-Prize by the Austrian Society of years’ investments. Particularly the publication of Allergology and Immunology (ÖGAI) for the paper several medical-oriented papers in high impact “An orthogonal proteomic-genomic screen identi- journals reflects the potential of the collaborations bet- fies AIM2 as a cytoplasmic DNA sensor for the ween CeMM and clinical departments of the MUV. inflammasome”, published in Nature Immuno- logy and the most highly cited basic medical re- Here are some examples: search paper authored in Austria in so far t Global target profile of the kinase inhibitor bosu- t ERC Advanced Investigator Grant awarded to tinib in primary chronic myeloid leukemia cells. Giulio Superti-Furga. With the €2m support, Remsing Rix LL, Rix U, Colinge J, Hantschel O, Giulio Superti-Furga and his team will study the Bennett KL, Stranzl T, Müller A, Baumgartner body’s immediate reaction to infections within C, Valent P, Augustin M, Till JH, Superti-Fur- the overall CeMM goal of bridging basic molecu- ga G. Leukemia. 2009 Mar;23(3):477-85. Epub lar biology and applied medicine. The project will 2008 Nov 27. start in spring 2010. (Fig. 1). t A common JAK2 haplotype confers susceptibili- ty to myeloproliferative neoplasms. Olcaydu D, In strategic and scientific questions, CeMM is advised Harutyunyan A, Jäger R, Berg T, Gisslinger B, by a Scientific Advisory Board of world-recognized, Pabinger I, Gisslinger H, Kralovics R. Nat Genet. international researchers: 2009 Apr;41(4):450-4. Epub 2009 Mar 15. t Prof. Dr. Richard Flavell (Chairman, Section of t Oxidation-specific epitopes are dominant tar- Immunobiology, Yale University School of Medi- gets of innate natural antibodies in mice and hu- cine, New Haven, USA) mans. Chou MY, Fogelstrand L, Hartvigsen K, t Prof. Dr. James D. Griffin (Chair, Department of Hansen LF, Woelkers D, Shaw PX, Choi J, Perk- Medical Oncology, Dana Farber Cancer Institute, mann T, Bäckhed F, Miller YI, Hörkkö S, Corr Boston, USA) M, Witztum JL, Binder CJ. J Clin Invest. 2009 t Prof. Dr. Carl-Henrik Heldin (Director, Ludwig In- May;119(5):1335-49. Epub 2009 Apr 13. stitute for Cancer Research, Uppsala University, SE) t TREM-1 activation alters the dynamics of pul- t Prof. Dr. Denis Hochstrasser (Head, Central monary IRAK-M expression in vivo and improves CeMM – Research Center for Molecular Medicine GmbH 13
Fig. 1: ERC Grant i-Five Approach: Interferon-focused Innate Immunity, Interactome & Inhibitome The proposed integrative proteomic approach aspires to derive a functionally-annotated map of the molecular machinery involved in viral re- ! ! " # - ` % O # # # ( ) ( # # # - standing of the interferon branch of molecular innate immunity.
host defense during pneumococcal pneumonia. use it to implement the development of innovative the- Lagler H, Sharif O, Haslinger I, Matt U, Stich rapeutic and diagnostic strategies (from the clinic to the K, Furtner T, Doninger B, Schmid K, Gattrin- clinic), it is important to foster existing collaborations ger R, de Vos AF, Knapp S. J Immunol. 2009 Aug and to start new projects. With a new research facility 1;183(3):2027-36. Epub 2009 Jul 13. situated in the heart of the AKH and MUV campus, the logistical advantage is an inherent part of CeMM‘s As it is one of the major goals of CeMM to combine opportunity and mandate to be successful in a field insight obtained from basic and clinical research and to where the clinical and scientific worlds meet. 14
Breath Research Institute
Head: Anton Amann
Aims and functions Results for 2008–2009 The Breath Research Institute of the Austrian Academy The analytical methods used at the institute allow a of Sciences is located in Dornbirn (Vorarlberg). It is an broad portfolio of applications: internationally oriented research center focusing on vola- t Exhaled breath analysis for medical diagnosis and tile compounds in exhaled breath, saliva, urine or sweat. therapeutic monitoring Analysis of exhaled breath is non-invasive, and therefo- t Real-time analysis of exhaled breath with ac- re well accepted by patients of every age. companying simulation of lung mechanics and hemodynamics The analytical repertoire used at the institute allows t Analysis of urine headspace detection of compounds at very low concentrations: t Search for entrapped persons based on compounds 1 part of a compound in 109 parts of air (1 part-per- released by breath, urine and sweat billion, 1 ppb). This corresponds to 1 cubic millimeter t Analysis of headspace of bacterial cultures as well in 1 cubic meter. Surprisingly, many different volati- as human cell cultures le compounds are observed in exhaled breath at this t Use of isotopically labelled precursor compounds concentration level. An example is isoprene, a hy- for personalized medicine. drocarbon, which is produced by plants as well as t Most prominent among the conducted projects mammals. In humans, it is produced as a by-product of were two EU-projects: the cholesterol synthesis. A typical concentration of iso- t The EU-project BA MOD (6th framework), dealing prene in exhaled breath of humans is around 100 ppb. with exhaled breath analysis of patients suffering Apart from volatiles from patients and volunteers, the from lung cancer and oesophageal cancer. institute also has an area of research dealing with in t The EU-project “Second Generation Locator for vitro investigations of volatiles released by bacteria or Urban Search and Rescue Operations” (SGL for cell lines. This gives complementary information, and USaR, 7th framework), investigating strategies may be used to elucidate metabolic pathways and bio- to detect entrapped persons after major disasters chemical origins of volatile compounds. (e.g. earthquakes, explosions or terror attacks). Volatiles in exhaled breath are sometimes compounds A related project for gas-analytical search operations which are produced during enzymatic detoxification of using proton tra nsfer reaction ma ss spectrometr y a nd other compounds by, e.g., cytochromes P450. The con- ion mobility spectrometry was funded by the Aust- centration pattern of volatiles in exhaled breath therefore rian Agency for Research and Development (FFG). is in part related to the activity spectrum of cytochrome P450 enzymes. In particular, inactivity of such enzymes The BAMOD lung cancer study was conducted in in- due to genetic polymorphisms may have a considerable tensive cooperation with Prof. Jochen Schubert’s Breath effect. Therefore the Breath Research Institute will build Research Group at the University of Rostock. In this up a research focus on “personalized medicine” which study we could not identify one particular molecular includes genetic information. The latter is complemen- species characteristic for lung cancer, but defined a panel tary to information on the phenotype as exemplified by of about 40 compounds, which arise in exhaled breath of the concentration pattern in exhaled breath. This focus lung cancer patients with increased probability or with on “personalized medicine” will also include research on increased concentration when compared to healthy vo- 13C-labelled compounds for non-invasive breath tests. lunteers (see Bajtarevic et al., BMC Cancer 9, see Fig. 1). Breath Research Institute 15
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In the field of urban search and rescue operations, An additional focus of research concerned the real- a major focus was laid on compounds released from time measurement of concentrations of isoprene and breath and urine and the investigation of their sprea- acetone in exhaled breath (see King et al., J Breath ding through different debris materials (see Fig. 2). Res 3, see Fig. 3) of volunteers under an ergometer challenge. Apart from exhaled breath, medical para- Another focus of research was the investigation of meters like the ECG, cardiac output and blood pressu- headspace of bacteria and human cell lines, the latter re were also recorded in these experiments. Isoprene is with a particular focus on lung cancer. appearing in breath in a relatively high concentration of ~100 parts-per-billion (ppb). We could demonstra- t We investigated the lung cancer cell lines NCI- te that even a small ergometer challenge (e.g. of 75 W) H2087, NCI-H1666, CALU-1 and A549 com- leads to a huge increase of isoprene concentration in paring them with healthy primary human breath by a factor of ~4. This increase in concentra- bronchial epithelial cells (HBEpC) and human fib- tion (from ~100 ppb to ~400 ppb) starts within a few roblasts (hFB). First results have been published in seconds and therefore is not expected to reflect a change Sponring et al. (Anticancer Res 29) and Filipiak in the metabolic synthesis rate of isoprene. Acetone et al. (Cancer Cell Int 8; Cancer Epidemiology shows a behavior which is entirely different from isop- Biomarkers and Prevention 19: 182). rene with no pronounced increase during an ergome- t Investigation of bacteria arising in ventilator- ter challenge (but similarities to the behavior of etha- associated pneumonia was started in a project nol). The observations in ergometer experiments were funded by the Austrian Agency for Research and corroborated by real-time analysis of exhaled breath in Development (FFG). the sleep laboratory. Since isoprene is a by-product of 16 Breath Research Institute
* + * ( # ( ( ( ? 0 cholesterol biosynthesis, such experiments and the t Buszewski, B., J. Nowaczyk, T. Ligor, P. Olszowsky, accompanying mathematical simulations of lung M. Ligor, B. Wasiniak, W. Miekisch, J. K. Schubert, mechanics and hemodynamics have potential for A. Amann: Preparation and characterization of mi- future therapeutic monitoring during operations or in croporous fibers for sample preparation and LC-MS an intensive care unit. Additional projects dealt with the determination of drugs. J. Separation Sci., 32, 2448- monitoring of valproic acid therapy or with breath analy- 2454, 2009; doi: 10.1002/jssc.200900094. sis of drug addicts. t Ligor, M., T. Ligor, A. Bajtarevic, C. Ager, M. Pi- Last but not least it is a central interest of the Breath enz, M. Klieber, H. Denz, M. Fiegl, W. Hilbe, W. Research Institute to contribute to the further Weiss, P. Lukas, H. Jamnig, M. Hackl, B. Buszew- development of the “Journal of Breath Research” ski, M. Miekisch, J. Schubert, A. Amann: Determi- (JBR), which is published by the Institute of Phy- nation of volatile organic compounds appearing in sics (IOP, Bristol, UK). The director of the Breath exhaled breath of lung cancer patients by solid pha- Research Institute is also Editor-in-Chief of the Journal se microextraction and gas chromatography mass of Breath Research, together with his colleague Prof. spectrometry. Clin. Chem. Lab. Med., 47, 550-560, Mel Rosenberg from Tel Aviv. 2009; doi: 10.1515/CCLM.2009.133. t Sponring, A., W. Filipiak, T. Mikoviny, C. Ager, J. Bibliography Schubert, W. Miekisch, A. Amann, J. Troppmair: t Bajtarevic, A., C. Ager, M. Pienz, M. Klieber, Release of volatile organic compounds from the K. Schwarz, M. Ligor, T. Ligor, W. Filipiak, H. lung cancer cell line NCI-H2087 in vitro. Antican- Denz, M. Fiegl, W. Hilbe, W. Weiss, P. Lukas, H. cer Res., 29, 419-426, 2009. Jamnig, M. Hackl, A. Haidenberger, B. Buszew- t Mochalski, P., B. Wzorek, I. Sliwka, A. Amann: ski, W. Miekisch, J. Schubert, A. Amann: No- Improved pre-concentration and detection me- ninvasive detection of lung cancer by analysis of thods for volatile sulphur breath constituents. exhaled breath. BMC Cancer, 9, 348, 16 p., 2009; J. Chromatogr. B, 877, 1856-1866, 2009; doi: doi: 10.1186/1471-2407-9-348. 10.1016/j.jchromb.2009.05.010. Breath Research Institute 17 t King, J., A. Kupferthaler, K. Unterkofler, H. Koc, the lung cancer cell line C A LU-1 in vitro. Cancer Cell S. Teschl, G. Teschl, W. Miekisch, J. Schubert, Int., 8, 17, 11 p., 2008; doi: 10.1186/1475-2867-8-17. H. Hinterhuber, A. Amann: Isoprene and acetone t Eisenmann, A., A. Amann, M. Said, B. Dat- concentration profiles during exercise at an ergo- ta, M. Ledochowski: Implementation and in- meter. J. Breath Res., 3, 027006, 16 p., 2009; doi: terpretation of hydrogen breath tests. J. Breath 10.1088/1752-7155/3/2/027006. Res., 2, 046002, 9 p., 2008; doi: 10.1088/1752- t Mochalski, P., B. Wzorek, I. Sliwka, A. Amann: 7155/2/4/046002. Suitability of different polymer bags for storage of t Krkošová, Ž., R. Kubinec, L. Soják, and A. Amann: volatile sulphur compounds relevant to breath ana- Temperature – programmed GC linear retention in-
lysis. J. Chromatogr. B, 877, 189-196, 2009; doi: dices of all C4 – C30 monomethylalkanes on methyl- 10.1016/j.jchromb.2008.12.003. silicone OV – 1 stationary phase. A contribution t Filipiak, W., A. Sponring, T. Mikoviny, C. Ager, J. towards a better understanding of volatile organic Schubert, W. Miekisch, A. Amann, J. Troppmair: compounds in exhaled breath. J. Chromatogr. A, 1179, Release of volatile organic compounds (VOCs) from 59-68, 2008; doi: 10.1016/j.chroma.2007.10.081.
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GMI – Gregor Mendel Institute of Molecular Plant Biology
Head: Magnus Nordborg
Aims and functions supported by its administration and a platform consist- The importance of plant biology is difficult to overstate. ing of the GMI’s own services, including state-of-the- Research on plants has led to many fundamental art plant growth facilities, as well as joint services with breakthroughs, from Gregor Mendel’s elucidation of the the Research Institute of Molecular Pathology and the basic principles of genetics, via Barbara McClintock’s Institute of Molecular Biotechnology. The GMI is em- discovery of transposons, to the recent work on bedded within the Vienna Biocenter Campus in pur- epigenetics and RNA silencing. During the last 20 pose-built premises and provides a lively, international years, the model plant Arabidopsis thaliana has emerged working environment, with employees from 22 coun- as a primary experimental system for modern molecular tries. The working language is English. In addition to biology. In addition, plants are the primary producers the core budget from the Austrian Academy of Sci- of the world’s ecosystems and are, thus, central to life ences, GMI researchers successfully attract substantial on earth, a fact to which rising food prices and a rapidly funding from the Austrian Science Fund, the EU, the changing climate have brought renewed attention. US National Institutes of Health and the US National Science Foundation. Research at the GMI, which is the only international centre for basic plant research in Austria, is carried out In 2009, Magnus Nordborg from the University of by independent research groups, led either by senior or Southern California, USA was appointed as Scientific junior group leaders. Research is curiosity driven and Director of the GMI. In his words, “The objective of covers many aspects of molecular genetics, including the GMI is to provide a world class environment for basic mechanisms of epigenetics, population genetics, basic research in plant biology — an environment in chromosome biology, developmental biology and stress which some of the most challenging and important signal transduction. The GMI’s research activities are questions of modern biology can be addressed”.
* + 8 A. thaliana D ( # ( + ( 9 Aquilegia. A. formosa ( ( A. pubescens ( ( GMI – Gregor Mendel Institute of Molecular Plant Biology <
Results for 2008–2009 targeting DNA methylation to specific regions of the genome by RNA-directed DNA methylation (RdDM) 1. Population genetics and in establishing blocks of silent chromatin that One of the most important challenges facing biology specify chromosome features such as centromeres. today is making sense of genetic variation. Understand- Forward genetic screens performed by the group of ing how genetic variation translates into phenotypic Marjori and Antonius Matzke have recently identified variation (Fig. 1) and how this translation depends on DMS3 (a structural maintenance of chromosome the environment is fundamental to our understanding hinge domain-containing protein), DMS5/NRPE1 of evolution, and has enormous practical implications (the largest subunit of Pol V), DMS6/DCL3 (DICER- for both medicine and agriculture. Magnus Nordborg LIKE3) and DMS4 (an IWR1-type transcription factor) studies a wide range of topics related to population ge- as important components of RdDM. Interestingly, in netics including the genetic basis of phenotypic varia- contrast to other dms mutants, dms4 shows defects not tion, molecular evolution, speciation and adaptation to only in RdDM but also developmental defects (Fig. 2). the environment. Although his empirical research fo- cuses on Arabidopsis thaliana, his group works on many The lab of Ortrun Mittelsten Scheid has characterised different organisms, including Aquilegia and African an epigenetic “double lock” silencing system that is green monkeys. As a world expert in genome-wide as- only alleviated upon simultaneous removal of DNA sociation (GWA) studies for studying natural variation, methylation and histone methylation. The group has Magnus Nordborg has pioneered this technology in A. further discovered significant changes in chromatin thaliana, including developing statistical methodology organisation upon prolonged heat stress, providing for dealing with confounding due to population struc- evidence that environmental conditions can override ture. His most recent work on a GWA study of 107 epigenetic control. phenotypes in a common set of A. thaliana inbred lines is in press in the journal Nature (see bibliography). As DNA sequence information is vital for GWA studies, Magnus Nordborg is involved in the 1001 genomes project (http://www.1001genomes.org/), which uses next generation sequencing in its aim to discover the whole-genome sequence variation in 1001 strains of the reference plant A. thaliana. This project is similar to the 1000 genomes project (http://www.1000genomes. org) investigating human genetic variation. Magnus Nordborg is a major partner of a National Institutes of Health (NIH)-funded Center of Excellence in Genomic Science that aims to investigate the regulatory networks by which genetic variation leads to phenotypic vari- ation. Magnus Nordborg is also a member of evolVi- enna, a virtual organisation created to bridge the 50 or so research groups based in Vienna studying various aspects of evolutionary biology.
2. Epigenetics Epigenetics, which can be defined as heritable changes that are not associated with differences in the DNA sequence, forms the focus of several research groups at the GMI. Silencing of genes through epigenetic modifications, such as DNA cytosine methylation, is essential for plant and animal development, as well as for protecting genomes from invasive sequences such as transposable elements and viruses. Recent work has uncovered important roles for small RNAs produced Fig. 2: Appearance of a dms4 through the RNA interference (RNAi) pathway in Arabidopsis thaliana 20 GMI – Gregor Mendel Institute of Molecular Plant Biology
Hisashi Tamaru’s lab has been investigating the epige- The blunt-ended telomeres are stabilised by Ku, a netic states of centromeric heterochromatin in sperm protein complex that is implicated in chromosome cell nuclei (SN) and vegetative cell nuclei (VN) of Ara- end protection in a wide range of organisms including bidopsis pollen and has discovered that vegetative nuclei humans. The Riha group has also revealed that despite invariably undergo extensive decondensation of centro- their heterochromatic features, Arabidopsis telomeres meric heterochromatin and lose centromere identity. are expressed and that some telomeric transcripts are processed by the RNA-directed DNA methylation 3. Telomeres and genome stability machinery into small heterochromatin siRNAs that Telomeres are indispensable elements of eukaryotic reinforce telomeric heterochromatin by mediating chromosomes that are important for the complete methylation of telomeric DNA. In recognition of his replication of linear genomes and for the stability achievements, Karel Riha was awarded the Novartis of chromosome ends. As a consequence, they have Research Prize in Biology as well as a START Prize important implications for human cancer and ageing. from the Austrian Research Fund in 2008. The key feature of telomeres is their ability to allow differentiation of native chromosome ends from Bibliography deleterious DNA double-strand breaks, which they t Atwell S, Huang YS, Vilhálmsson BJ, Willems G, do by forming complex capping structures (Fig. 3). Horton M, Li Y, Meng D, Platt A, Tarone A, Hu Karel Riha has made the fundamental discovery of TT, Jiang R, Muliyati NW, Zhang X, Amer MA, a novel mechanism of chromosome end protection. Baxter I, Brachi B, Chory J, Dean C, Debieu M, The Riha lab has discovered that a substantial portion de Meaux J, Ecker JR, Faure N, Kniskern JM, Jones of Arabidopsis telomeres end with a blunt end and JDG, Michael T, Nemri A, Roux F, Salt DE, Tang not with a 3’ single stranded protrusion which is C, Todesco M, Traw MB, Weigel D, Marjoram important for the formation of so called t-loops. P, Borevitz J, Bergelson J, Nordborg M (2010)
Fig. 3: Model illustrating the formation of a chromosome end protective cap in Arabidopsis and human cells. After DNA replication, the lagging telomere acquires a G-overhang, while the leading telomere is blunt-ended. In humans, the blunt end is processed by a nuclease [1], which allows formation of t-loops at both sides of a chromosome [2]. In Arabidopsis, the blunt end is stabilised by a cap that includes Ku [3]. In the absence of O ( ( 0 ( Q # S=U V ? 0 SWU % ( ( SXU GMI – Gregor Mendel Institute of Molecular Plant Biology 21
Genome-wide association study of 107 phenotypes Transgenerational stress memory is not a general in a common set of Arabidopsis thaliana inbred response in Arabidopsis. PloS ONE 4(4):e5202. lines. Nature (in press). t Kanno T, Bucher E, Daxinger L, Huettel B, t Kanno T, Bucher E, Daxinger L, Huettel B, Kreil Böhmdorfer G, Gregor W, Kreil DP, Matzke M, DP, Breinig F, Lind M, Schmitt MJ, Simon SA, Matzke AJ (2008) A structural-maintenance-of- Gurazada SG, Meyers BC, Lorkovic ZJ, Matzke AJ, chromosomes hinge domain-containing protein Matzke M (2009) RNA-directed DNA methylation is required for RNA-directed DNA methylation. and plant development require an IWR1-type Nature Genet 40:670-675. transcription factor. EMBO Rep 11:65-71. t Nordborg M, Weigel D (2008) Next-generation t Daxinger L, Kanno T, Bucher E, van der Winden J, genetics in plants. Nature 456:720-723. Naumann U, Matzke AJ, Matzke M (2009) A stepwise t Daxinger L, Kanno T, Matzke M (2008) Pol V pathway for biogenesis of 24-nt secondary siRNAs and transcribes to silence. Cell 135(4):592-594. spreading of DNA methylation. EMBO J 28:48-57. t Jones AM, Chory J, Dangl JL, Estelle M, Jacobsen t Schoft VK, Chumak N, Mosiolek M, Slusarz L, SE, Meyerowitz EM, Nordborg M, Weigel D Komnenovic, V, Brownfield L, Twell D, Kakutani T, (2008) The impact of Arabidopsis on human health: Tamaru H (2009) Induction of RNA-directed DNA diversifying our portfolio. Cell 133:939-943. methylation upon decondensation of constitutive t Riehs N, Akimcheva S, Puizina J, Bulankova P, Idol heterochromatin. EMBO Reports 10:1015-1021. RA, Siroky J, Schleiffer A, Schweizer D, Shippen DE, t Pecinka A, Rosa M, Schikora A, Berlinger M, Riha K. (2008) Arabidopsis SMG7 protein is required Hirt H, Luschnig C, Mittelsten Scheid O (2009) for exit from meiosis. J Cell Sci 121:2208-2216. 22
IMBA – Institute of Molecular Biotechnology GmbH
Head: Josef Penninger
Aims and Functions cluding all postdoctoral and student projects are an- The IMBA - Institute of Molecular Biotechnology is a nually evaluated by the Scientific Advisory Board of subsidiary of the Austrian Academy of Sciences located at the IMBA. The current SAB members are: Eric Kan- the Campus Vienna BioCentre, a cluster of research ins- dell, winner of the Nobel Prize for molecular control titutes and companies dedicated to excellence of research of memory; Guenther Blobel, winner of the Nobel (http://www.imba.oeaw.ac.at). IMBA is a joint initiative Prize for protein targeting; Kenneth Chien, Director of the Austrian Academy of Sciences and Boehringer In- of the Cardiovascular Research Center Department gelheim governed by a supervisory board and sharing of Cell Biology at Massachusetts General Hospital; all infrastructures.. IMBA is directed by Josef Penninger Tony Hyman, Max Plank Institute, Dresden, Susan (Scientific Director), Michael Krebs (Administrative Di- Lindquist, previous Director of the Whitehead Insti- rector) and Jürgen Knoblich (Deputy Scientific Director tute, MIT, Cambridge, and HHMI investigator; Gary with full power of attorney). Ruvkun, co-discoverer of microRNAs and recipient of When we started IMBA in 2003 the challenge was how the Lasker Award 2008, Massachusetts General Hos- to develop a new research institute that is indeed compe- pital, Boston; and Fiona Watt, a world famous stem titive at the international level. In the last years, IMBA cell researcher, London. IMBA is also recognized as an has developed into the largest institute of the Austri- excellent place for training students and provides mul- an Academy of Science in both personnel and budget. tidisciplinary training opportunities for students and Importantly IMBA scientists have already performed post-doctoral fellows in advanced molecular genetics, groundbreaking research. As an example, ~ 50% of all functional mouse genomics, immunology, RNA bio- publications that directly come from Austrian institu- logy, stem cell biology, or molecular pathology (www. tions in the top scientific journals Nature, Science, and imba.oeaw.ac.at/career). Cell have been published by IMBA/IMP researchers in Based on the current expertise and international the last 5 years; half of this scientific output comes from standing of IMBA investigators, the laboratory space IMBA. Moreover, in the last 5 years the Wittgenstein available, the faculty size required to increase compe- prize, the top science prize in Austria has been awarded titiveness and “buffer” faculty turn-over, and in order twice to researchers working at IMBA and two senior to develop a strong and recognizable research profile and one junior ERC grants have been awarded to IMBA we propose in the mid-term to hire 1 more Senior researchers. IMBA researchers also cooperate with other Scientist and 4 more Junior PIs with a focus on the research centres and have been invited to literally hund- three following topics: reds of lectures at meetings including multiple keynote t Disease Modeling and Mouse Genetics and distinguished lectures and in some cases lectures on t Cell and Stem Cell Biology public policies and lectures to children. t RNA Biology and Epigenetics The philosophy and business model of IMBA was al- ways to hire the best young scientists in the world and Results for 2008–2009 to provide them with academic freedom and free access The mammalian system for controlling bone remodelling to the best possible infrastructure. Thus, IMBA has in- also regulates fever (Hanada et al. Nature 2009) deed been able to attract some of the best young minds. The so-called RANK protein and the molecule that IMBA currently has 3 Senior Scientists and 5 Junior binds to it, the RANK ligand or RANKL, have formed Principal Investigators (PIs). All science at IMBA in- a focus of Penninger’s work since 1999, when his group IMBA – Institute of Molecular Biotechnology GmbH 23
* + Y Y );98 Z O ( # * + );98! ^ O deleted the RANKL gene from mice and showed that control of body temperature. Unlike normal mice, mice the RANK/RANKL system was the “master regula- that had been engineered to lack RANK in the brain did tor” governing bone loss (Kong et al. 1999 Nature 402, not respond to simulated infections by raising their body 304-309). The work provided the fist genetic proof for temperature although they appeared otherwise normal. a completely new and rational treatment for osteoporo- Taken together, these results show that RANK and its sis, one of the most serious public health problems for ligand are involved in the regulation of the body’s fever older women. The results of phase III clinical trials for response to fight infections. a human antibody to RANKL have recently been pu- Because Penninger’s group had previously shown that blished (see Cummings et al. 2009, New Eng. J. Med. RANK and RANKL control the production of milk 361, 756-765) and, pending approval by the authori- during pregnancy, it seemed possible that the system ties, it is conceivable that this antibody will soon be might also have an effect on the body temperature made widely available for osteoporosis treatment. of females. Indeed, female – but not male – mice la- Considering that such treatments might be of potential cking RANK in the brain show a significant increase benefit to millions of patients, it is important to under- in body temperature compared with their littermates, stand any possible side-effects. The function of RANK at least during daylight hours. As a result, such female and RANKL in the brain was completely unknown. To mice have much lower differences in body tempera- investigate it, Reiko Hanada - an endocrinologist and ture between day and night. Because the experimen- Postdoc in Penninger’s group - injected RANKL into tal work was performed in mice and rats, it seemed mice and rats, intending to look for effects on behavi- extremely likely that the results would be relevant to or. The results were dramatic and obvious – the animals other mammals, including man. Proof that this is the stopped moving and developed really high temperatures. case arose when the IMBA group learned of a family RANK and RANKL are not present in all areas of the whose children had defects in the RANK gene. As brain. Rather, the proteins were found to be restricted to predicted, these children showed much lower fever areas that other groups had previously implicated in the responses to infection. Even when they contracted 24 IMBA – Institute of Molecular Biotechnology GmbH pneumonia, their body temperatures scarcely rose. This quantum leap is the result of a new procedure with This data links bone metabolism to the control of tem- which makes it possible to analyse complex biological perature during infection and, even less expectedly, to processes in all the genes of the fruit fly simultaneously. the gender-specific control of body temperature. It is This unusually extensive gene analysis was only made conceivable that the RANK/RANKL system may be possible by using the IMP-IMBA fly library, which was responsible for the sudden bursts of high temperature compiled by neurobiologist Barry Dickson. The data- associated with hormonal changes – and with osteo- base contains 20,000 strains of flies and in each of the- porosis – in older women. se exactly one gene is deactivated. The experiments were performed at the IMBA in close This amounts to a paradigm shift as far as medical pro- collaboration with groups at the Medical University of Vi- gress is concerned. When faced with a clinical picture, enna as well as in Berlin and Shuh Narumiya in Japan. the old approach was to search for the responsible gene, the proverbial needle in a haystack. In principle, all ge- Genome-wide analysis of Notch nes are now known, and the question is a much more signalling in Drosophila by transgenic RNAi systematic one: what exactly do the genes related to the (Mummery-Widmer et al. Nature 2009). illness control in the body? In future it will be possib- Our genes determine how we look, but also what illnes- le to tackle the identification of the causes of illnesses ses we suffer from. Since the beginning of this century, even more systematically. all human genes have been identified. But what function does each gene have in the organism? For humans this The first genome wide screen on innate immunity question will remain unanswered for some time to come, (Cronin et al. Science 2009) but for a model organism like the fruit fly an answer is A paper by Shane Cronin et al. (Science 2009) descri- already possible. A new approach enabled IMBA resear- bes the first genome-wide in vivo Drosophila RNAi cher Jürgen Knoblich to investigate the function of genes screen to isolate genes which control the fly’s response across an organism’s whole genotype simultaneously. to ingested pathogenic bacteria. It took more than five
Fig. 2: Leonie Ringrose, Junior Group Leader at IMBA, having a conversation with one of her students. * + );98! ^ O IMBA – Institute of Molecular Biotechnology GmbH 25 years and involved around two million data points to Penninger. Central control of fever and female find out which of the ~ 13,000 fly genes are involved body temperature by RANKL/RANK. Nature, in innate immunity. 462, 505-509. 2009. Contrary to acquired immunity which is a feature of t Mummery-Widmer, J. L., Yamazaki, M., Stoeger, mammals, innate immunity is a fast, non-specific re- T., Novatchkova, M., Bhalerao, S., Chen, D., action to external pathogens and is found in all animal Dietzl, G., Dickson, B. J., and Knoblich, J. A. organisms. In flies, it involves the activity of intestinal (2009). Genome-wide analysis of Notch signal- defenses, antimicrobial peptides and macrophage-like ling in Drosophila by transgenic RNAi. Nature cells in the blood. 458, 987-992. The screen took advantage of the Vienna Drosophila t Cronin, SJ., Nehme, NT., Limmer, S., Liegeois, RNAi Library, a powerful tool of 22,000 strains of S., Pospisilik, JA., Schramek, D., Leibbrandt, A., fruit flies located at IMBA. The experiment included Simoes, Rde M., Gruber, S., Puc, U., Ebersberger, feeding the flies with the highly infectious bacterium I., Zoranovic, T., Neely, GG., von Haeseler, A., Serratia marcescens to which immunocompetent flies Ferrandon, D., Penninger, J. M. (2009). Genome- usually succumb within six days. IMBA researchers wide RNAi screen identifies genes involved in were able to switch off the fly’s genes one by one, using intestinal pathogenic bacterial infection. Science a standardized heat shock procedure as trigger. 325(5938):340-345. In addition to previously known genes and pathways, t Schwamborn, J. C., Berezikov, E., and Knoblich, the experiments revealed more than 800 additional ge- J. A. (2009). The TRIM-NHL protein TRIM32 nes involved in innate immunity, the majority of which activates microRNAs and prevents self-renewal in had unknown function until now. Intriguingly, some mouse neural progenitors. Cell 136, 913-925. ten percent of these genes seem to have a negative func- t Imai, Y., Kuba, K., Neely, G. G., Yaghubian-Mal- tion against infection: when they were switched off, the hami, R., Perkmann, T., van Lool, G., Ermolaeva, infected flies actually lived longer than the expected six M., Veldhuizen, R., Leung, Y. H. C., Wang, H., days, in some cases much longer. For the other 90% of Liu, H., Sun, Y., Pasparakis, M., Kopf, M., Mech, immune-compromised fruit flies, life was significantly C., Bavari, S., Peiris, J. S. M., Slutsky, A. S., Aki- shortened by one or two days. ra, S., Hultqvist, M., Holmdahl, R., Nicholls, J., Because of the high degree of conservation of innate Jiang, C., Binder, C. J., and Josef M. Penninger. immunity, the results are also relevant for immunolo- Identification of oxidative stress and Toll like re- gists interested in higher animals and humans. One ceptor 4 signaling as a key pathway of acute lung of the next steps will therefore be to find out which injury. Cell 133, 235-249. 2008. of the isolated genes have relevance in the context of t Aronica L, Bednenko J, Noto T, DeSouza LV, mammalian immunity. Siu KW, Loidl J, Pearlman RE, Gorovsky MA, The work was carried out in collaboration with Dominique Mochizuki K. (2008), Study of an RNA helicase Ferrandon and Nadine Nehme of the Institut de Biologie implicates small RNA-noncoding RNA interac- Moleculaire et Cellulaire du CNRS in Strasbourg. tions in programmed DNA elimination in Tet- rahymena, Genes Dev. 22(16):2228-41. Bibliography t Koestler SA, Auinger S, Vinzenz M, Rottner K, t Pospisilik, J. A., Schramek, D., Cronin, S. J. F., Small J. V., (2008), Differentially oriented popu- Nehme, N. T., Todoric, J., Bayer, M., Orthofer, lations of actin filaments generated in lamellipo- M., Neely, G. G., Dietzl, G., Manoukian, A., Fu- dia collaborate in pushing and pausing at the cell novics, M., Prager, G., Wagner, O., Ferrandon, D., front, Nat Cell Biol. 3:306-13. Epub 2008 Feb 17. Hui, C.-C., Esterbauer, H., and J. M. Penninger. t Neumuller, R. A., Betschinger, J., Fischer, A., Drosophila whole genome mining reveals Hedge- Bushati, N., Poernbacher, I., Mechtler, K., Co- hog-signaling as a key regulator of brown/white hen, S. M., and Knoblich, J. A. (2008). Mei- adipose cell fate. Cell, Jan 8. 2010. P26 regulates microRNAs and cell growth in t Hanada, R., Leibbrandt, A., Hanada, T., Kitao- the Drosophila ovarian stem cell lineage. Nature ka, S., Furuyashiki, T., Fujihara, H., Trichereau, 454, 241-245. J., Paolino, M., Qadri, F., Plehm, R., Klaere, S., t Wirtz-Peitz, F., Nishimura, T., and Knoblich, J. A. Komnenovic, V., Mimata, H., Yoshimatsu, H., (2008). Linking cell cycle to asymmetric division: Takahashi, N., Haeseler, A., Bader, M., Kilic, A. Aurora-A phosphorylates the Par complex to S., Ueta, Y., Pifl, C., Narumiya, A., and J. M. regulate Numb localization. Cell 135, 161-173. 26
Institute for Biomedical Aging Research
Head: Beatrix Grubeck-Loebenstein
Aims and Functions Results for 2008–2009 The Institute for Biomedical Aging Research (IBA) focuses on all questions related to biogerontology and Immunology Group is the only one of its kind in the whole of Austria. In the period under report, the group has specialized Worldwide, the segment of the population aged 60 in studying the restructuring of the CD8 T cell and over has been increasing rapidly. repertoire in elderly persons. Of high interest in this regard is the result that oxidative modifications In 2001, in Austria more than one-fifth of the modulate the antigenicity of immunodominant population was over 60 years of age and by 2030, this peptides. This demonstrates that immune reactions segment will have risen to one third. As a result of drastically change through oxidative stress situations, the predicted demographic changes, aging research which often occur in old age. Another interesting has been gaining more and more importance to define result is that authophagy is an essential mechanism new approaches to meet age-related needs. for the survival of T lymphocytes in old age and that autophagy is induced by polyamines. The goal of biomedical research on aging is to help people grow old with dignity and in good health. Endocrinology Group Basic research at the IBA comprises the analyses The group’s work put an emphasis on molecular of aging processes at the cellular level to better mechnanisms which underly the biological aging of understand age-related impairments and diseases the male, particularly the prostate. Hereby genes and and furthermore, to postpone/prevent age-related their protein products, which are responsible for tissue impairments and diseases in order to improve the reorganisation of the aging prostate, were identified. quality of life in old age. At the moment, there are The studies focus on the proteins Dickkopf 3 (DKK3) six research groups at the institute, who put all their and PAGE-4. effort into decoding molecular mechanisms of cellular aging in different models and analyzing their impact Stem Cell and Extracellular Matrix Research Group on the differentiation and function of cells. The group made it possible to prove for the first time that human mesenchymal stem cells in old age exist in Research activities at the IBA are supported by numer- the same number but with decreased quality and that the ous national and international co-operations. This way, functional loss is mainly due to chronic inflammatory the institute successfully collaborates with the Tyrolean stimuli. Within a large scale animal experiment, which Universities and Health Authorities. Additionally, the studied the effects of radiotherapeutic treatment to institute coordinates the FWF sponsored national re- mesenchymale stem cells in vivo, the unexpected result search network “Proliferation, differentiation and cell was obtained that very high doses of radiation cause death in aging cells” (NFN S9301) and participates in permanent damage hardly or not at all. eight different EU-projects. In collaboration with the Medical University Innsbruck, the IBA coordinates Cell Metabolism and Differentiation Research Group the PhD-program for Biogerontology (“The aging of The group’s aim is to study the role of caloric restriction biological communication systems”), which is of high (CR) in human fat cells. Caloric restriction is the only importance to its students. known intervention that reduces the rate of biological Institute for Biomedical Aging Research 27 aging. In the period under report, the molecular impact Fig. 1: Staining of the mitotic spindle of the “CR mimetic” resveratrol as well as new CR apparatus and cytoplasmic tubulin mimetics, namely inhibitors of metabolic key regulators, ( ( part of the cytoskeleton, in red, in hu- were identified. Furthermore, it was shown that a man epithelial cells. decrease in the retinol-binding protein-4 level through CR contributes to an improved regulation of the glucose-metabolism and increases insulin sensitivity.
Aging of Smooth Muscle Research Group In this research area, the molecular mechanisms of the framework of the above mentioned NFN, it was the function of the smooth muscle filament Myorod possible to show that four microRNAs, namely miR-17, were discovered. miR-19b, miR-20a and oR-106a are commonly down- regulated in different models of cell aging. Decreases Molecular and Cell Biology Group in these miRNAs correlated with increased transcript An especially interesting finding within the research levels of some established target genes, especially the activity of this group was the proof that oxygen radicals, cdk inhibitor p21/CDKN1A. These results establish which are produced through the NADPH oxidase Nox4, miRNAs as novel markers of cell aging in humans. have a major impact on the aging of endothelial cells. Therefore, it can be assumed that if Nox4 is blocked Bibliography pharmacolocically, the aging of cells and possibly tissue t Spoden, G. A., S. Mazurek, D. Morandell, N. can be influenced. In another experiment the group Bacher, M. J. Ausserlechner, P. Jansen-Dürr, E. Ei- showed a functional connection between age dependent genbrodt, W. Zwerschke: Isotype-specific inhibi- changes of mitochondrial function and the function of tors of the glycolytic key regulator pyruvate kinase the proteasom, an organelle which is used to eliminate subtype M2 moderately decelerate tumor cell pro- damaged proteins. These results clarify the mechanisms liferation. Int. J. Cancer, 123, 312-321, 2008; doi: of skin aging in human beings. 10.1002/ijc.23512. t Kloss, F. R., R. Gassner, J. Preiner, A. Ebner, K. NFN-cooperation Larsson, O. Hächl, T. Tuli, M. Rasse, D. Moser, K. Through intense collaboration of research groups Laimer, E. A. Nickel, G. Laschober, R. Brunauer, at the IBA with other Austrian research groups in G. Klima, P. Hinterdorfer, D. Steinmüller-Nethl,
* + \ + z + { |D8 > Institute for Biomedical Aging Research
Abb. 4: T-Zellen
Fig. 3: miRNA array of immune cell sub-populations from young and elderly donors.
G. Lepperdinger: The role of oxygen termination t Lener, B., R. Koziel, H. Pircher, E. Hütter, R. of nanocrystalline diamond on immobilisation of Greussing, D. Herndler-Brandstetter, M. Her- BMP-2 and subsequent bone formation. Biomate- mann, H. Unterluggauer, P. Jansen-Dürr: The rials, 29, 2433-2442, 2008; doi:10.1016/j.biomate- NADPH oxidase Nox4 restricts the replicative rials.2008.01.036. lifespan of human endothelial cells. Biochem. J., t Weinberger, B., D. Herndler-Brandstetter, A. 423, 363-374, 2009; doi: 10.1042/BJ20090666. Schwanninger, D. Weiskopf, B. Grubeck-Loeben- t Eisenberg, T., H. Knauer, A. Schauer, S. Büttner, stein: Biology of immune responses to vaccines in C. Ruckenstuhl, D. Carmona-Gutierrez, J. Ring, S. elderly persons. Clin. Infect. Dis., 46, 1078-1084, Schroeder, C. Magnes, L. Antonacci, H. Fussi, L. 2008; doi: 10.1086/529197. Desczc, R. Hartl, E. Schraml, A. Criollo, E. Mega- t Zenzmaier, C., G. Untergasser, M. Hermann, S. lou, D. Weiskopf, P. Laun, G. Heeren, M. Breiten- Dirnhofer, N. Sampson, P. Berger: Dysregula- bach, B. Grubeck-Loebenstein, F. Herker, B. Fahren- tion of Dkk-3 expression in benign and malignant krog, K.-U. Fröhlich, F. Sinner, N. Tavernarakis, N. prostatic tissue. Prostate, 68, 540-547, 2008; doi: Minois, G. Kroemer, F. Madeo: Induction of au- 10.1002/pros.20711. tophagy by spermidine promotes longevity. Nat. Cell t Zenzmaier, C., J. Marksteiner, A. Kiefer, P. Berg- Biol., 11, 1305-1314, 2009; doi: 10.1038/ncb1975. er, C. Humpel: Dkk-3 is elevated in CSF and t Weiskopf, D., A. Schwanninger, B. Weinberger, plasma of Alzheimer’s disease patients. J. Neuro- G. Almanzar, W. Parson, S. Buus, H. Lindner, B. chem., 110, 653-661, 2009; doi: 10.1111/j.1471- Grubeck-Loebenstein: Oxidative stress can alter 4159.2009.06158.x. the antigenicity of immunodominant peptides. Institute for Biomedical Aging Research <
Fig. 4: The small worm C.elegans is an important tool in aging research: Picture from the recently established C.elegans facility at the IBA.
J Leukoc. Biol., 87, 165-172, 2010; [epub ahead of Eckhard, E. Tschachler, C. Papak, M. Scheideler, print 2.10.2009] doi: 10.1189/jlb.0209065. Z. Trajanoski, R. Grillari-Voglauer, B. Grubeck- t Hackl, M., S. Brunner, K. Fortschegger, C. Loebenstein, P. Jansen-Dürr, J. Grillari: miR-17, Schreiner, L. Micutkova, C. Mück, G. Laschober, miR-19b, miR-20a and miR-106a are down- G. Lepperdinger, N. Sampson, P. Berger, D. regulated in human aging. Aging Cell, 9, 291- Herndler-Brandstetter, M. Wieser, H. Kühnel, 296, 2010; [epub ahead of print 18.01.2010] doi: A. Strasser, M. Breitenbach, M. Rinnerthaler, L. 10.1111/j.1474-9726.2010.00549.x. 30
Institute for Biophysics and Nanosystems Research
Head: Peter Laggner
Aims and Functions structures concerned lie below the limit of resolution Biophysics is the essential link between biochemistry of the light microscope, and hence their visualization and cell biology. While biochemistry focuses primarily requires special techniques of X-ray diffraction and on the molecular chemical reactions underlying the scattering. The development and implementation of essential biological functions, such as metabolism, new X-ray techniques at synchrotron radiation facili- energy transformation and information storage, ties as well as in the routine laboratory, is a well-rec- cell biology concentrates on the whole cell as an ognized speciality of the IBN. integral element of living and dying systems. Between these poles lie at least four orders of magnitude in The knowledge gained from biophysical and nanosys- dimensions. The dominating question to be answered tems research has important, fundamental relevance to bybiophysics, is therefore: how does structural self- biomedical and pharmaceutical applications. On the organization of billions of molecules in a nanosystem other hand, new approaches towards the design of nano- lead to a living microorganism? One may safely use biotechnological devices, e.g. in the bio-functionaliza- the analogy between the single person and its manifold tion of supramolecular systems for the use in diagnostics interactions and synergies within the society that is or synthesis, result from the research at the IBN. more than the sum of its individuals. The research projects of the IBN are embedded in nu- The objectives of the IBN within this broad pursuit merous national and international programmes, such are well defined: the principles and forms of molecu- as the Austrian Nano-Initiative (NanoHealth), the lar self-organization, especially of lipids and proteins programmes of the FWF, and EU framework pro- within the aqueous medium of the cytoplasm are at grammes. The IBN-Outstation at the synchrotron the focus of research. These entities play key roles in light source ELETTRA in Trieste is a world-wide rec- compartmentation and transport within the cell. The ognized research facility heavily used by the interna- tional scientific community (Fig. 1).
Results for 2008–2009
Working group R. PRASSL (Lipoproteins and Lipid Nanoparticles) The Prassl working group has continued its efforts to elucidate structural elements of ApoB100. The dynamics of ApoB100-containing lipoprotein fractions were investigated by elastic incoherent neutron scattering (ILL, Grenoble). Specific differences in the temperature dependency of the effective force constants of LDL and VLDL were assigned. While the crystallization trials on detergent solubilized ApoB100 were intensified, Fig. 1: The IBN operates a beamline for X-ray Nanoanalytics at the self-assembling short-chain peptides were explored Synchrotron light source ELETTRA, Trieste – Italy. with the goal to stabilize the lipid-free protein. Institute for Biophysics and Nanosystems Research 31
Fig. 2: Design and synthesis of multifunctional liposomes for medical diagnostics: Stealth liposomes are enriched with synthesized lipid-derivati- # ( ? #
The fabrication of multifunctional liposomes for lipid composition of the target membrane insertion of medical diagnostics was successfully improved. Various antimicrobial peptides leads to a variety of membrane biomolecules (i.e. lectin, adiponectin, regulatory defects (significant changes in bilayer thickness, peptides and antibodies) were covalently linked elastic properties, supramolecular aggregation and to liposomes for specific cellular recognition. The pore formation), which in turn can induce membrane linkage of marker molecules (Gd3+, radionuclides or disruption. These peptides, derived from a fragment fluorescence labels) now enables the visualization of of human lactoferricin, which also exhibit endotoxin the functionalized liposomes with different imaging neutralizing activity, have been tested in pre-clinical modalities. Alternatively, experiments to incorporate studies within a spin-off originating from this project. ironoxide nanoparticles into liposomes for magnetic resonance imaging have been initiated. In collaboration with the Center of Medical Research in Graz a new specific surface marker (phosphatidylserine) The development of a liposomal drug delivery system has been found for a number of cancer cell lines (Fig. for the aerosolic application of vasoactive intestinal 3), which can be a target for membrane-active peptides. peptide (VIP) was successfully completed Therefore within an EC and a national research project highly specific peptides towards this marker will be Working group Karl Lohner (Functional Lipidomics) developed, which present highly potent candidates The Lohner working group is mainly engaged in for new anticancer drugs. the development of new agents against antibiotic resistant bacteria and related sepsis as well as against Working group H. Amenitsch (synchrotron radiation; cancer. These new agents are derived from human Outstation at ELETTRA, Trieste) host defense peptides. The focus relates to the The Amenitsch working group is in charge of the elucidation of the molecular mode of action of the Austrian SAXS-Beamline at ELETTRA which newly designed agents on model- and cell membranes. is integrated as a user-facility in international Biophysical studies showed that depending on the synchrotron research. In the frame of the EU-project 32 Institute for Biophysics and Nanosystems Research
e.g. during apoptosis) on membrane dynamics and structure. We found significant changes to the lateral heterostructure of membranes that are large enough to affect the functioning of membrane proteins through a mechanical coupling. (Fig. 4a) A second focus is the development of a lab X-ray technique: the recently developed small-angle X-ray (SAXS) camera with a 50 W micro-source has been further optimized. Currently swapping the optics from line- to point-focus (and vice versa) can be done easily. Furthermore, new sample stages have been add- ed, among them a high-pressure X-ray cell to measure samples under pressures of up to 1000 bar. Optionally the camera can also be equipped with a high-precision stepper motor, allowing us to measure the surface structure of thin films in the grazing incidence mode (“GISAXS”), a beam geometry where the incident X- ray beam probing the sample is almost parallel to the surface of the sample (as opposed to the conventional “transmission” mode).
* "+ * 0 ( ( Another goal is to complement the camera with a 8 0 8 0 =>> # * DSC-(differential scanning calorimetry) sample stage transmission + (DSC-SAXS) enabling us to monitor simultaneously + thermodynamic and nanostructural changes of a sample line SBCL2 bottom: prostate cancer cell line LNCaP. undergoing a thermotropic phase transition (Fig. 4b)
Bibliography t Pabst, G., B. Boulgaropoulos, E. Gander, B. R. Sa- SAXIER (http://www.saxier.org/), methodological rangi, H. Amenitsch, V. A. Raghunathan, and P. developments in the fields of microfluidics and gas Laggner: Effect of ceramide on nonraft proteins.J. phase analysis were performed and the project was Membrane Biol., 231, 125-132, 2009; doi: 10.1007/ successfully terminated. Essential achievements in s00232-009-9211-3. regard to single particle structural analysis were reached by the combination of laser-tweezers and synchrotron X-ray diffraction. Promising approaches regarding the interaction of single particle were continued with experiments on single starch grains. Furthermore, the investigations on the nanoscopic structural changes of the collagen in human arteries were concluded and the project has been completed.
Working group P. Laggner (Physical Chemistry) The Laggner research group focuses on the development and the implementation of new methods and concepts in the field of nanosystems research. Thus, this group provides the basis for the other working groups at the IBN with emphasis on the structural and the dynamic properties of lyotropic liquid crystalline * =+ D % 0 0 phases and their phase transitions. In the past two The inset shows a simulation of the hydrodynamic focusing inside the years, research has mainly focused on the effects of 0 ( D # D8D # ceramides (sphingolipids enzymatically generated # / /3 particle growth. Institute for Biophysics and Nanosystems Research 33
* W + @ / - D; ( ( D; D; |/ membrane structure were found to be large enough to affect the activ- ity of membrane channels.
Fig. 5b: Compact high-performance small-angle X-ray camera in ( + D" ; + WV ( # ( - # 0 > 0 W | # by courtesy of Hecus X-ray Systems GmbH, Graz, Austria. t Hodzic, A., P., Rappolt, H. Amenitsch, P. Laggner, terial activity of lactoferricin derivatives. Biochem. and G. Pabst: Differential modulation of mem- Biophys. Res. Comm., 369, 395-400, 2008: doi: brane structure and fluctuations by plant sterols 10.1016/j.bbrc.2008.01.176. and cholesterol. Biophys. J., 94, 3935-3944, 2008; t Sevcsik, E., G. Pabst, W. Richter, S. Danner, H. doi: 10.1529/biophysj.107.123224. Amenitsch, and K. Lohner: Interaction of LL- t Stark, B., F. Andreae, W. Mosgöller, M. Edets- 37 with model membrane systems of different berger, E. Gaubitzer, G. Köhler, and R. Prassl: Li- complexity – influence of the lipid matrix. Bio- posomal vasoactive intestinal peptide for lung ap- phys J., 94, 4688-4699, 2008; doi: 10.1529/bio- plication: Protection from proteolytic degradation. physj.107.123620. Eur. J. Pharm. Biopharm., 70, (1) 153-164, 2008; t Shyjumon, I., M. Rappolt, B. Sartori, H. Amen- doi: 10.1016/j.ejpb.2008.04.01. itsch, and P. Laggner P.: Novel in situ setup to study t Prassl, R., M. Pregetter, H. Amenitsch, M. the formation of nanoparticles in the gas phase by Kriechbaum, R. Schwarzenbacher, and M. J. small angle x-ray scattering. Rev. Sci. Instrum., 79, Chapman: Low density lipoproteins as circu- 043905, 5 p., 2009; doi: 10.1063/1.2908436. lating fast temperature sensors. PlosOne, 3(12): t Falcaro, P., L. Malfatti, H. Amenitsch, B. Mar- e4079, 6 p., 2008. mioli, G. Grenci, and P. Innocenzi: Fabrication t Prassl, R., and P. Laggner: Molecular structure of of advanced functional devices combining soft low density lipoprotein. Current Status and Future chemistry with X-ray lithography in One Step, Challenge. Eur. Biophys. J., 38, (2) 145-158, 2009; Adv. Mater., 21, (48) 4932-4936, 2009; doi: doi: 10.1007/s00249-008-0368-y. 10.1002/adma.200901561. t Lohner, K.: New strategies for novel antibiotics: t Arsov, Z., M. Rappolt, and J. Grdadolnik: peptides targeting bacterial cell membranes. Gen. Weakened hydrogen bonds in water confined Physiol. Biophys., 28, 105-116, 2009; doi: 10.4149/ between lipid bilayers: The existence of a long- gpb_2009_02_105. range attractive hydration force. Chem. Phys. t Zweytick, D., S. Tumer, S. E. Blondelle, and K. Chem. 10, 1438-1441, 2009; doi: 10.1002/ Lohner: Membrane curvature stress and antibac- cphc.200900185. 34
Konrad Lorenz Institute for Ethology
Head: Dustin Penn
Aims and Functions Academy of Sciences and the City of Vienna. Below are The Konrad Lorenz Institute for Ethology (KLIVV) is some highlights of recent publications: a research institute for the study of animal behavior. It t Zala and Penn found that female mice prefer the was founded in 1945 and named after Konrad Lorenz scent of males genetically resistant to infectious who played a central role in pioneering the field of agents (N-ramp “knock-ins”), and resistant males ethology (also known as behavioral biology). The have better ability to maintain high testosterone institute has five senior scientists, nine post-doctoral during infection than susceptible ones (Zala et al. scientists (supported mostly by external funding), 14 2008). They also found that elevated testosterone support staff, and several graduate students. KLIVV is levels, induced by exposing males to female scent, highly international, as the scientists and students come does not suppress immunity, contrary to what is from many different countries (Fig. 1). generally assumed, though it increases the ener- getic costs of resolving infection. The research addresses interdisciplinary questions con- t Ilmonen and Penn investigated the mechanisms cerning both the proximate mechanisms and the evo- that control life-history trade-offs between sur- lution of behavior. The scientists study a diversity of vival and reproduction in wild house mice. They species, especially vertebrates (including H. sapiens), in previously found that elevated reproduction and the laboratory, in seminatural conditions, and in the social stress cause attrition of telomeres, the field. KLIVV has unusually good facilities for studying DNA-protein complexes on the ends of chromo- animals in captivity, close access to the field, and a fully somes that control genomic integrity and cellular equipped molecular genetic laboratory. senescence. Recently, they showed that repeated exposure to infectious agents also reduces tel- omere length, which may contribute to senes- The main topic of interest is sexual selection, as it po- cence (Ilmonen et al. 2008). tentially explains many puzzling aspects of behavior, t Ilmonen and Penn previously found that even such as the complex and elaborate courtship displays moderate levels of inbreeding causes significant found in many species. Furthermore, sexual selection reductions in fitness in wild mice, and they began provides an example of how behavior can generate investigating whether inbreeding reduces males’ genetic and evolutionary changes. In addition to con- mating success only through male-male competi- ducting basic research on animal behavior, the institute tion or also female choice. Recently, they found aims to apply insights from ethology to better under- that inbred females prefer the scent of genetically stand the behavior of our own species – and address outbred versus inbred males, suggesting that in- applied problems in conservation and the environment. bred females may have more to gain than outbred females by mating with outbred, heterozygous Results for 2008–2009 males (Ilmonen et al. 2009). KLIVV scientists published 50 papers in international, t Hettyey and Hoi investigated behaviors that fe- peer-reviewed journals – which is a new record for the male frogs use to cope with sexual coercion and institute, and several publications received attention sperm competition. They studied two related and from the international press. Y. Moodley (2009 Science) sympatric species where heterospecific mating received the Best Paper Award for 2009 by the Austrian results in inviable offspring, as the fitness conse- Konrad Lorenz Institute for Ethology 35
* + O) quences of mating with a male of a wrong spe- recommendations for sustainable management of cies are severe. They found that females recognize this endangered species (Schaub et al. 2009). when they are mounted by heterospecific males, and they have subtle, yet effective means to avoid KLIVV scientists obtained three grants from the the complete loss of a year’s reproductive effort Austrian National Science Foundation (FWF), one (Hettyey et al. 2009) (Fig. 2). by Wagner and two by Hoi. Hoi also received a grant t Hoi and Griggio used bearded tits to test the from the Austrian Ministry of Sciences’ Sparkling armament-ornament dual utility model, which Science program. predicts that females exploit the same signals males use in aggressive contests, and they found To commemorate the Darwin Year in 2009, KLIVV that beard length plays an important role in both scientists promoted evolutionary biology through pub- male-male competition and female choice (Hoi & lic outreach and education (Fig. 3). Griggio 2009). t Wagner and his colleagues found that kittiwake Bibliography gulls tend to mate with genetically dissimilar t Hettyey, A., S. Baksay, B. Vági, H. Hoi: Counter- individuals, and that such pairs produce more strategies to sexual coercion by heterospecifics in heterozygous offspring with enhanced survival female frogs. Anim. Behav., 78, 1365-1372, 2009; (Mulard et al. 2009). They previously found doi: 10.1016/j.anbehav.2009.09.006. that female gulls preferentially utilize new ver- t Hoi, H., and M. Griggio: Dual utility of a mel- sus old sperm from their mates to fertilize eggs, anin-based ornament in bearded tits. Ethol- and more recently they found evidence that old ogy, 114, 1094-1100, 2008; doi: 10.1111/j.1439- sperm causes hatching failure, slower embry- 0310.2008.01566.x. onic development and poor hatchling condition Fig. 2: Female frogs suf- (White et al. 2008). fer enormous reproduc- t Wagner and colleagues found experimental evi- tion costs when mating dence that an invertebrate (Drosophila) possesses with a male of a wrong the cognitive ability to exploit public information species. when choosing their mates (Mery et al. 2009). t Beissmann and colleagues examined the prob- lem of reintroducing captive-bred Alpine bearded vultures into the wild. They estimated survival probabilities and fecundity to project population size and extinction risk in the future, and made 36 Konrad Lorenz Institute for Ethology t Ilmonen, P., G. Stundner, M. Thoß, and D. J. cally similar matesis mal-adaptive in a monoga- Penn: Females prefer the scent of outbred males: mous bird. BMC Evol. Biol., 9, 147, 12 p., 2009; good-genes-as-heterozygosity? BMC Evol. Biol., 9, doi: 10.1186/1471-2148-9-147. 104, 10 p., 2009; doi: 10.1186/1471-2148-9-104. t Schaub, M., R. Zink, H. Beissmann, F. Sarrazin, t Ilmonen, P., A. Kotrschal, and D. J. Penn: Telomere R. Arlettaz: When to end releases in reintroduction attrition due to infection. PLoS ONE 3(5): e2143, programmes: demographic rates and population 6 p., 2008; doi: 10.1371/journal.pone.0002143. viability analysis of bearded vultures in the Alps. t Mery, F., S. A. M. Varela, E. Danchin, S. Blan- J. Appl. Ecol., 46, 92-100, 2009; doi: 10.1111/j.1365- chet, D. Parejo, I. Coolen, R. H. Wagner: Public 2664.2008.01585.x. versus personal information for mate copying in an t White, J., R. H. Wagner, F. Helfenstein, S. A. invertebrate. Curr. Biol., 19, 730-734, 2009; doi: Hatch, H. Mulard, L. C. Naves, E. Danchin: 10.1016/j.cub.2009.02.064. Multiple deleterious effects of experimentally aged t Moodley, Y., B. Linz, Y. Yamaoka, H. M. Windsor, sperm in a monogamous bird. P. Natl. Acad. Sci. S. Breurec, J.-Y. Wu, A. Maady, S. Bernhöft, J.-M. USA, 105, (37) 13947-13952, 2008; doi: 10.1073/ Thiberge, S. Phuanukoonnon, G. Jobb, P. Siba, D. Y. pnas.0803067105. Graham, B. J. Marshall, M. Achtman: The peopling t Zala, S. M., B. K. Chan, St. D. Bilbo, W. K. Potts, of the Pacific from a bacterial perspective. Science, R. J. Nelson, D. J. Penn: Genetic resistance to 323, 527-530, 2009; doi: 10.1126/science.1166083. infection influences a male’s sexual attractiveness t Mulard, H., E. Danchin, S. L. Talbot, A. M. and modulation of testosterone. Brain Behav. Ramey, S. A. Hatch, J. F. White, F. Helfenstein, Immun., 22, (3) 381-387, 2008; doi: 10.1016/j. R. H. Wagner: Evidence that pairing with geneti- bbi.2007.09.003.
* "+ * Y 8 ^ - posium, entitled Darwin and Behavioural Sciences, at the Conference / Q 9 | ( @# ; + 8 Q / !@# / # +!! ! ##! # ! ( ( European Society for Evolutionary Biology, as part of the society’s efforts to improve public education and counter intelligent design and other forms of creationism. 37
Earth Sciences
Institute for Geographic Information Science
Head: Josef Strobl
Aims and Functions Competence in spatial communication and spatial think- The Institute for Geographic Information Science ing is the foundation for the practical use of geospatial ‘GIScience’ is focussed on basic research for information in business and daily life. Transfer into and Geoinformatics. Spatial Analysis and Spatial Data through schools is therefore actively supported, with the Infrastructures are core themes complemented by additional objective of fostering interest in academic transversal work on the modeling of space-time phenomena programmes within the science and technology fields. and the introduction of ‘thinking spatially’ to learners from target groups in formal and informal education. Results for 2008–2009 Our goal is to advance Geographic Information Science Geographic Information Science is considered the through interdisciplinary research with a strong focus theoretic basis and conceptual foundation for geoin- on conceptual and methodological aspects. The formatics as a methodology. GIScience researches the ÖAW Institute for GIScience intends to be a leading representation as models, the organisation, analysis research entity recognized for its contributions to and visual communication of all kinds of georefer- and international leadership in the foundations of enced information. It thus serves as the foundation Geoinformatics. This is being achieved by addressing for applications of Geographic Information Systems carefully selected key topics at the leading edge of (‘GIS’) in industry, public administration and in the international research in GIScience. personal sphere of individuals. Space and Time The aim of spatial analysis is to extract information One group within the GIScience Institute explores from georeferenced empirical data. Research objectives the cognitive, social and operational aspects of space are directed at advanced remote sensing image analysis, at the geostatistical analysis of 3D and 4D data sets and the modeling and simulation of dynamic processes.
Spatial Data Infrastructures (SDI) are complex architec- tures supporting the full spectrum of Geo-ICT applica- tions. The implementation of distributed geo-databases requires an extensive set of continuously evolving stand- ards. The GIScience unit contributes to current research on metadata, catalog services and portal development in collaboration with national and international consortia.
Integrated Space-Time analysis is based on extended * + (% 9 ) 8 + "Y - data models, and requires dedicated analytical strat- | egies as well. Research aims are directed at the de- # ( ; / (% 9 ) 8 !# 0 ) "Y velopment of new analytical operators for spatiotem- 0 + ( poral data as well as their validation across various # ( ? ( application contexts. # ; | ^ "> Institute for Geographic Information Science
* + D 8 + (% # ( LuLc-Class if their aspect is different by > | ^
and time in GIScience. This transversal research topic geostatistics and the regionalization of dynamic is a common base for many team members. Examples processes. In December 2009, we submitted an of research in 2008-2009 are the spatio-temporal “FWF” application for funding a stand-alone project modeling of natural reforestation considering the researching the heterogeneous knowledge of GIScience GIScience perspective, or global scale trends analysis geoinformatics and image processing capabilities. based on a worldwide statistical database. Another A major new initiative was launched in the area of research focus is on analyzing existing and developing object-based image analysis; OBIA – 3D and 4D new geovisualization concepts for the mapping of modeling (paper accepted by the International Journal change. This cooperative work within the GIScience of Remote Sensing). Institute explores options to visualize and analyze the dynamic properties of geo-related processes. Learning to Think Spatially Successful acquisition and communication of spatial Spatial Data Infrastructures knowledge is required across all segments of society. In Our research contributes to the specification of 2009 the research focus was on providing knowledge interoperable services like metadata and catalog on collaborative learning environments with a specific implementations and to advanced multi-dimensional emphasis on value added by spatial representation data models, the integration of real-time sensor input and collaborative vizualisation. After successfully and open interfacing across systems architectures. In completing a project within the national “Sparkling 2009 we have submitted the first report for the FP7 Science” initiative, we have recently successfully project within the eContent+ framework; NatureSDIplus, applied for a two year Sparkling Science Project entitled a project which contributes to the INSPIRE initiative. “Geovisualisation and Communication in Participatory Decision Processes”. Spatial Analysis, Modelling and Simulation Research questions address segmentation-based Bibliography information extraction from remotely sensed imagery t Ahamer, G., A. Car, R. Marschallinger, G. and computer tomography as well as multidimensional Wallentin, F. Zobl: How to map perspectives.
* "+ D Y ) + ( \ * * Institute for Geographic Information Science "<
Fig. 4: Time and Space: The TREELIM model was develo- ped in the NetLogo modelling framework to simulate tree line Q V
Ubiquitous Comput. Commun. J. (UbiCC), 4, (3) mammal teeth using micro CT and object based 609-617, 2009;. image analysis, in: Computational Vision and t Car, A., O. Dahlman, B. Andersson, and P. Zeil: Medical Image Processing, J. M. R. S. Tavares and Games and scenarios in the context of GMOSS, R. M. Natal Jorge (Eds.), pp. 395-399, CRC Press, in: Remote Sensing from Space: Supporting Boca Raton, Florida, USA, 464 p., 2009. International Peace and Security, B. Jasani, M. t Drăguţ, L., T. Schauppenlehner, A. Muhar, J. Pesaresi, S. Schneiderbauer and G. Zeug (Eds.), pp. Strobl, and T. Blaschke: Optimization of scale 71-86, Springer, Netherlands, 297 p., 2009; doi: and parametrization for terrain segmentation: An 10.1007/978-1-4020-8484-3_6. application to soil-landscape modeling. Comput. t Fischer, F.: Volunteered Geographic Information Geosci., 35, (9) 1875-1883, 2009; doi: 10.1016/j. - Baustein zukünftiger Geoinformations- cageo.2008.10.008, 2008. infrastrukturen?, in: Geokommunikation im Umfeld t Wallentin, G., U. Tappeiner, J. Strobl, and E. der Geographie, Karel Kriz, Wolfgang Kainz und Tasser: Understanding alpine tree line dynamics: Andreas Riedl (Hrsg.), Wiener Schriften zur Geo- An individual based model. Ecol. Model., 218, 235- graphie und Kartographie 19, Wien, 218 S., 2009. 246, 2008; doi: 10.1016/j.ecolmodel.2008.07.005. t Hofmann, P., J. Strobl, and T. Blaschke: t Jekel, T., Die Macht der Karten und die Macht der Quantifying the robustness of fuzzy rule sets in Kinder. Versuch einer Begründung des Lernens object based image analysis. Int. J. Remote Sens., mit Geoinformation, in: kind : macht : raum, K. accepted, 2010. Dobler, T. Jekel and H. Pichler (Hrsg), S. 62–75, t Marschallinger, R., Ch. Eichkitz, H. Gruber, Wichmann, Heidelberg, 173 S., 2008. K. Heibl, R. Hofmann, and K. Schmid: The t Lang, S., S. Schoepfer, D. Hoelbling, T. Blaschke, Gschliefgraben Landslide (Austria): A Remediation M. Moeller, T. Jekel, E. Kloyber: Quantifying Approach involving Torrent and Avalanche and Qualifying Urban Green by Integrating Control, Geology, Geophysics, Geotechnics and Remote Sensing, GIS and Social Sciences, in: Geoinformatics. Austrian J. Earth Sci., 102, (2) 36- Use of Landscape Sciences for the Assessment of 51, 2009. Environmental Security, Petrosillo et al. (Eds.), pp. t Hofmann, P., R. Marschallinger, and G. Daxner- 93-105, Springer Press, Netherlands, 497 p., 2008; Höck: 3D volume modelling of fossil small doi: 10.1007/978-1-4020-6594-1_6. 40
Center for Geosciences
Head: Hans P. Schönlaub
Aims and Functions Results for 2008–2009 The Center for Geosciences (GWZ) was founded Prospective interdisciplinary research topics within the in 2008 as a managing organization for a uniform four earth scientific commissions have already been representation of the earth sciences both within the discussed by the members of the GWZ. Since no joint Austrian Academy of Sciences and in public. Besides scientific activities have been carried out so far, the having its own responsibilities, the center encompasses reports for 2008-2009 will be separate ones for each and coordinates the following commissions of the of the commissions. A joint report for the geoscience Section for Mathematics and the Natural Sciences of activities of the academy will be submitted from hope- the academy that focus on the earth sciences: fully 2010 onwards. t Commission for the Paleontological and Stratigra- In 2008 two meetings of the center were held. Besides phical Research of Austria general topics, such as reports of the center and the t Commission for Geophysical Research commissions involved, space and personnel questions t Commission for Basic Research on Mineral Raw and logistical aspects, a number of specific items were Materials discussed during these meetings: t Commission for Quaternary Research. t By-laws of the Center for Geosciences t Scientific Advisory Board of the Center for Geo- The duties of the Center for Geosciences according to sciences the by-laws include the following: t Administrative and logistical support of the geos- t Identifying innovative geoscientific research topics cience commissions by the Center for Geosciences t Initiating and supporting interdisciplinary geosci- t Financial administration of the geoscience com- entific research projects missions t Presenting a uniform face and coordinatiing the t AkademIS (the science information system of the earth sciences both within the academy as well as academy) in public t Eduard Suess Lectures 2009-2010 t Organizing outreach activities in the geosciences t Strategy-Workshop Geosciences 2009 t Aiding the scientific commissions in some of their t Joint Symposium of the geoscience commissions of administrative duties the academy during 2009 t Preparing a joint annual report for the geoscience t Attendance of various scientific conferences in activities of the academy. Austria and abroad.
The members of the Center for Geosciences include a A draft of the by-laws, as well as the list of the pro- head and a deputy head, as well as the chairmen and posed members of the Scientific Advisory Board, were deputies of the four geoscience commissions of the acad- prepared during the fall 2008 meeting held jointly emy. They meet at least twice a year for consultations. with the members of the GWZ and both were sub- The Center for Geosciences has a Scientific Advisory mitted to the Presiding Committee of the Academy. Board (SAB), which consists of six renowned interna- In addition, the needs and wishes of the various com- tional geoscientists. The SAB provides advice and con- missions that are part of the center were canvassed trol for the center on scientific topics and support in and administrative support according to the limited performing its duties. capacities of the center was agreed on. All financial Center for Geosciences 41 aspects remain the responsibility and duty of each re- spective commission, as the commissions receive their budget directly from the academy.
At the beginning of 2009 the Presiding Committee of the Academy approved the by-laws and the members of the Scientific Advisory Board of the GWZ. The in- augural meeting of the SAB including the election of the chair and vice-chair was held in May 2009. During the subsequent Geoscience Symposium and Strategy- Workshop current research activities and main results of the commissions were presented and innovati- ve research topics of social relevance were discussed. Prospective thematic cooperation has been identified within topics concerning paleoclimate, Quaternary geology and raw material issues, geophysics and in the Carnian Stage of the Late Triassic.
The executive board of the GWZ was engaged in planning and organizing the “Eduard Suess Lectures” (named after the former President of the Academy and famous Austrian geologist). The first two of the total six high-quality lectures dealing with geoscience topics of public interest and importance were held in October and December 2009 (http://www.oeaw.ac.at/ shared/news/2009/info_suess_lectures.html).
In connection with the current discussion on restruc- turing of the academy, a proposal for “Perspectives 2020” for the Center for Geosciences was requested by the Presiding Committee of the Academy at the end of September 2009. The critical statement, prepared by the GWZ executive board, should be understood to be the initiation of a discussion process and due to time constraints this paper could not be coordinated with the heads of the four commissions or with the Science Advisory Board of the GWZ.
Bibliography As a consequence of the current structure and the lim- ited resources, no publications of the Center for Geo- sciences are available at this time. 42
Commission for the Palaeontological and Stratigraphical Research of Austria
Head: Werner E. Piller
Aims and Functions Results for 2008–2009 The Commission for the Palaeontological and Stratigraphical Research of Austria (CPSA) focuses on Reef-Ecosystems the two basic topics in earth sciences, palaeontology and Within the project Upper Triassic reefs of the Northern stratigraphy. It aims to foster basic scientific knowledge Calcareous Alps work at the internationally famous in this field of science but also to provide its sound model reef of the Steinplatte (Tyrol/Salzburg) was documentation and presentation. finished in 2008 and published in 2009 (B. Kaufmann). The original goal of the commission was the edition For the first time the complex subsidence/uplift history of the “Catalogus Fossilum Austriae” (CFA), which of this carbonate platform has been demonstrated. represents a systematic encyclopaedia of all described Besides this study another model reef of the Northern and indicated fossils on Austrian territory. Besides this Calcareous Alps, the Adnet Reef (Salzburg), has been particular aim of the commission the field of activity, studied as part of a joint project with the TU Berlin. however, has considerably broadened during the last This study focussed on the diagenetic history of this years. In direct connection with the fossil documentation reef and was able to demonstrate a complex sequence of in CFA the database “OETYP” has been established to karstification and flooding events of this reef by eustatic provide public online access to all palaeontological types sea level changes and tectonics. and figured materials of Austrian fossil collections. Starting with 2006, the CPSA put its main emphasis The project Devonian Reefs of Austria was continued with on Palaeoecosystems as a prime scientific research target a focus in the Carnic Alps (T. J. Suttner). One of the and considers the identification of specific Austrian topics was the study of subaeral exposure and erosion topics out of this very broad scientific field as a mission. of reefs during the Middle Devonian and the resulting The scientific researchers have to adopt innovative sedimentary gap spanning from the Middle Devonian methods in their particular projects and these have to to the Lower Carbonifereous (Fig. 1). These studies were be integrated into international perspectives, however, part of IGCP project 503, Early Paleozoic Palaeogeo- an Austrian component has still to be visible. graphy and Palaeoclimate, which provided additional In Stratigraphy a broad spectrum should be covered funding and an international frame for the project. out of this wide thematic field. The basis for this is the “Stratigraphic Chart of Austria 2004 (sedimentary Cenozoic Palaeoecosystems of Austria sequences)” which includes most lithostratigraphic The project Stratigraphy and Facies in the Lower Miocene units on Austrian territory (http://www.uni-graz.at/ of the Molasse Zone was carried out in cooperation gepwww/forschung/Stratigraphische_Tabelle_von_ with the Rohöl Aufsuchungs AG (RAG) by funding a Oesterreich_2004.pdf). These units have to be described Ph.D.-project (P. Grunert). Within this project, drill- and documented in detail, they also have to be put cores of Lower Miocene sediments of the Molasse into an international context and properly evaluated. Basin of Upper Austria and Salzburg are under study The database “LITHSTRAT” acts as a documentation with respect to stratigraphy and facies (Fig. 2). The tool for the units. Besides this basic work also modern palaeontological focus deals with foraminifers and stratigraphic principles and methods have to be applied dinoflagellate cysts. In addition geochemical proxies are to Austrian rocks and sediments, to develop a broad also under study. To enhance the general stratigraphic based integrated stratigraphy in Austria. base for the Molasse Zone, the type locality for the Commission for the Palaeontological and Stratigraphical Research of Austria 43
* + | # ( ? ; ^ / 8 ) Y # succession a reworking horizon indicates a sedimentary gap, spanning the middle Devonian to the Lower Carboniferous. regional chronostratigraphic stage of the Ottnangian, Publication projects Ottnang-Schanze, has been re-studied and re-evaluated The documentation of all described fossil fishes from (M. Harzhauser, NHM Vienna; W. E. Piller, Univer- Austrian territory within the CFA has been continued sity of Graz; S. Coric, Geological Survey Vienna). An by O. Schultz (NHM Vienna). additional study deals with the spectacular finding of the sunfish Austromola angerhoferi. This megafossil was Cooperations and Meetings found together with other micro- and megafossils in a In cooperation with the Italian Stratigraphic Commission “Konservat Lagerstatte” near Pucking (Upper Austria). evaluation and revision of the Paleozoic lithostratigraphic The current project deals with a palaeoceanographic and units of the Carnic Alps has been initiated. Cooperation environmental reconstruction of the site and also with with the Chinese Academy of Sciences in Nanjing on the the specific fossilisation conditions of this Lagerstatte. Lower/Middle Devonian of Xinjang has been continued Work on the catalogue on dinoflagellate cysts has been as well as the study of “Devonian Biotic Events” with continued in the Lower Miocene but has been extended the Czech Geological Survey. into the Oligocene (A. Soliman, University of Graz). In July 2008 the international workshop “Methods in The project on Palaeolimnology has been intensified by Ostracodology” was organized by D. Danielopol, M. collaboration between D. Danielopol (free collaborator), Gross und W. E. Piller in Graz in cooperation with M. Harzhauser (NHM Vienna), W. E. Piller (University colleagues from Spain and Great Britain. In September of Graz) and M. Gross (Universalmuseum Joanneum, 2009 the internationally attended “Paleozoic Seas Graz) on the Upper Miocene Lake Pannon. Detailed Symposium” was held in Graz organized by T. J. analyses of drill-cores have been carried out which allow Suttner, B. Hubmann, W. E. Piller. the detection of environmental changes on a decadal scale. These analyses have been performed with fossils Bibliography (ostracods, molluscs) and using also geochemical/ t Belmecheri, S., T. Namiotko, C. Robert, U. Von geophysical proxies. Grafenstein, and D. L. Danielopol: Climate controller preservation in Lake Ohrid (Albania, Database projects Macedonia). Palaeogeogr. Palaeocl., 277, 236-245, Work on the databases “OETYP” (coordinator: A. 2009; doi: 10.1016/j.palaeo.2009.04.013. Kroh, NHM Vienna) (http://www.oeaw.ac.at/oetyp/ t Danielopol, D. L., M. Artheau, and P. Marmonier: palhome.htm) and “LITHSTRAT” (coordinator: I. Site prioritisation for the protection of rare Zorn, Geological Survey Vienna) has been continued. subterranean species – the cases of two ostracods 44 Commission for the Palaeontological and Stratigraphical Research of Austria
Fig. 2: Characteristic clayey-sandy silts with small- scaled cross-bedding from the Eggenburgian Hall * O^ D P ^ ( 8
from south-western France. Freshwater Biol., glacial sediments of Lake Mondsee (Austria). 54, 877-884, 2009; doi: 10.1111/j.1365- Crustaceana, 82, 1209-1212, 2009; doi: 2427.2008.02033.x. 10.1163/156854009X454612. t Humphreys, W. F., L. S. Kornicker, and D. L. t Reinhold, C., and B. Kaufmann: Sea-level changes Danielopol: On the origin of Danielopolina as controlling factor of early diagenesis: The reefal baltanási sp. n. (Ostracoda, Thaumatocypridoidea) limestones of Adnet (Late Triassic, Northern from three anchialine caves on Christmas Calcareous Alps, Austria). Facies, 56, 231-248, Island, a seamount in the Indian Ocean. 2010; doi: 10.1007/s10347-009-0197-1. Crustaceana, 82, 1177-1203, 2009; doi: t Soliman, A., T. J. Suttner, A. Lukeneder, and 10.1163/156854009X423157. H. Summesberger: Dinoflagellate cysts and t Kaufmann, B: The Steinplatte complex (Late Ammonoids from Upper Cretaceous sediments Triassic, Northern Calcareous Alps, Austria) – of the Pemberger Quarry (Krappfeld, Carinthia, subsidence-controlled development of a carbonate- Austria). Ann. Naturhist. Mus. Wien A, 110, 401- platform-to-intrashelfbasin-transition. Acta Geol. 421, 2009. Polonica, 59, (3) 341–357, 2009. t Suttner, T. J.: An ozarkodinid conodont cluster t Korn, D., and B. Kaufmann: A High-Resolution from Kirchfidisch (Lower Devonian, Austria). Relative Time Scale For The Viséan Stage Ann. Naturhist. Mus. Wien A, 111, 233-244, 2009. (Carboniferous) Of The Kulm Basin (Rhenish t Suttner, T. J.: Lower Devonian conodonts of Mountains, Germany). Geol. J., 44, 306-321, the “Baron von Kottwitz” quarry (Southern 2008; doi: 10.1002/gj.1143. Burgenland, Austria). Palaeontogr. Am., 62, 75-87, t Namiotko, T., D. L. Danielopol, M. Pichler, and 2009. U. Von Grafenstein: Occurrence of an Arctic t Suttner, T. J., B. Hubmann, and W. E. Piller: Paleo- ostracod species, Fabaeformiscandona harmsworthi zoic Seas Symposium. Berichte des Institutes für (Scott, 1899) (Ostracoda, Candonidae) in late Erdwissenschaften 14, Universität Graz, 94 S., 2009. 45
Commission for Geophysical Research
Head: Michael Kuhn
Aims and Functions Together with two existing Austrian glacier inventories The Commission for Geophysical Research that (1969 and 1998) the obtained laser scanning data gives originally included the Commission for Seismics as the opportunity to quantify area and volume changes well as the Commission for Aero-electric Research was over the past almost 40 years at three dates for 81 glaciers founded in 1952. In 1991 it was combined with the (116 km2) in the Ötztal Alps which accounts for more Commission for High-alpine Research and renamed than a quarter of Austria’s glacier extent (Fig. 1). Glacier “Commission for Geophysical Research”. area and volume have reduced drastically with significant differences within the individual size classes. Between The scope of this commission has been concentrated on 1997 and 2006 an overall area loss of 10.5 km2 or 8.2% the subjects of glaciology, seismics and gravimetry with occurred. Volume has been reduced by 1.0 km3 which its focus on the Austrian glacier inventory, contributions accounts for a mean thickness change of 8.2 m. The to international projects in depth-reflection seismics in availability of three comparable inventories (1969, 1998 the Alps as well as gravimetry-measurements in Austria. and 2006) allows a comprehensive analysis of glacier The glaciology project Austrian Glacier Inventory with changes over all size classes but lacks a high temporal a 3d-inventory (ice- thickness - area - volume) gained resolution. We therefore used glacier length as well as special international importance. The gravimetric map mass balance measurements from all available glaciers of Austria counts as another outstanding contribution within the study area to analyse the potential course of of the Commission for Geophysical Research. glacier changes in terms of area and volume. Extracting the course of changes out of this additional data material Results for 2008–2009 allows a rough estimation of mean annual area and volume changes and thus acceleration trends. We found, The Austrian glacier inventory that mean annual volume and thickness losses have Besides the statistical evaluation of the 1998-inventory, increased considerably more than mean area changes. we applied a simple, reliable and time-economic method to derive recent changes in glacier area and volume A further step in understanding regionally different by taking advantage of high resolution laser scanning acceleration trends used meteorological reanalysis DEMs (digital elevation models) from the year 2006. data (ERA40) and a gridded precipitation dataset
* + @0 boundary delineation as well as calculated volume changes on Rotmoos- and V
* + ; ( - tion Austria. 46 Commission for Geophysical Research
reflection data of the line TR0503 gained from the ÖAW projects “Development of geophysical methods for the spatial investigation of time-variant movements of rock masses: ÖAW Research Programme Geophysik der Erdkruste (GdE)” and “Integrated geophysical studies of Alpine inhomogeneous mass movements Gschliefgraben / Upper Austria: ÖAW Research Fig. 3: Course of the Karl Eder in the Traunsee. Programme International Strategy for Disaster Reduction (ISDR)” were processed.
(HISTALP). Fig. 2 shows mean annual winter The data acquisition was carried out in March 2005; the precipitation and all Austrian glaciers. The mass data were recorded using a seismic instrument mounted balance model driven with this data is able to reproduce at the Karl-Eder vessel. As energy source a water-gun measured mass balance changes well and allows for was used. The positions were determined by a GPS implementation on unmeasured glaciers. A higher receiver mounted at the Karl-Eder vessel recording the temporal resolution as well as a reconstruction of time and position data in a 2 seconds interval (Fig. 3). mass balances back to 1950 is the result of this study. The data were processed by ProMAX, a processing The temporal as well as spatial variability of tuning package used worldwide primarily for the exploration parameters has been investigated and interpreted. of Oil- and Gas reservoirs. In a first step the line was partitioned in clearly interpretable sections, 7 west- Marine Seismic Survey Traunsee, Processing Line east and 5 north-south aligned profiles (Fig. 4a). The TR0503 geodesic data necessary for processing were determined Within the framework “Deep reflection seismic” using the GPS recordings. The signal/noise ratio was and “Neotectonic analysis of seismic data”, seismic significantly increased using true amplitude recovery,
Fig. 4: Stack Sections and V Y Commission for Geophysical Research 47
t Escher-Vetter, H., M. Kuhn, and M. Weber: Four decades of winter mass balance of Vernagt- ferner and Hintereisferner, Austria: Methodology and results. Ann. Glaciol., 50, 87-95, 2009; doi: 10.3189/172756409787769672. t Knoll, C., H. Kerschner, and J. Abermann: Devel- opment of South Tyrolean glaciers since the Little Ice Age maximum. Zs. Gletscherkunde Glazialgeol., 42, (1) 19-36, 2009. t Kuhn, M., A. Lambrecht, J. Abermann, G. Patzelt und G. Gross 2009: Die österreichischen Glet- scher 1998 und 1969. Flächen- und Volumenän- derungen, Kommission für die wissenschaftliche Zusammenarbeit mit Dienststellen des Bundesmi- * W+ 8 V nisteriums für Landesverteidigung, Projektbericht Z 8( D ( > 10, Verlag Österreichische Akademie der Wissen- schaften, Wien, 128 S., 2009. water-gun delay correction and noise reduction t Kuhn, M., J. Abermann, M. Bacher and M. Olefs: processes; a big challenge in the process was the The transfer of mass balance profiles to unmeas- considerable amount of monofrequent noise. Through ured glaciers. Ann. Glaciol., 50, (1) 185-190, 2009; stacking the final, interpretable sections as shown in doi: 10.3189/172756409787769618. Fig. 4b were created. The depth map depicted in Fig. 4c t Kuhn, M., J. Abermann, M. Olefs, A. Fischer, and is a by-product of the water-gun delay correction; it A. Lambrecht: Gletscher im Klimawandel: Aktu- was established transforming seismic travel times into elle Monitoringprogramme und Forschungen zur depth by the use of seismic water velocities estimated Auswirkung auf den Gebietsabfluss im Ötztal, from the data. The final interpretation is currently in Mitteilungsblatt des Hydrographischen Dienstes in progress and will provide new insight concerning the Österreich, 86, 31-48, 2009. geologic realities close to the Gschliefgraben. t Kuhn, M.: Klimawandel und Gletscherschwund, in: Auswirkungen des Klimawandels auf die Bibliography österreichische Wasserwirtschaft, Bundesministerium t Abermann, J., A. Lambrecht, A. Fischer, and M. für Land- und Forstwirtschaft, Umwelt und Kuhn: Quantifying changes and trends in glacier Wasserwirtschaft und Österreichischer Wasser- area and volume in the Austrian Ötztal Alps (1969– und Abfallwirtschaftsverband (Hrsg.), S. 129–140, 1997–2006). The Cryosphere, 3, 205-215, 2009. Wien, 204 S., 2009. =>
Commission for Quaternary Research
Head: Gernot Rabeder
Aims and Functions the Middle Pleistocene (approx. 700,000 years before The main aim of the commission is to promote research present). These conclusions are based on the extremely which has a connection to the Quaternary period. well preserved skull and postcranial remains of the Included fields of research are: glaciology, palaeontology, bears from Deutsch-Altenburg. anthropology, geology of the Quaternary, dating, and palaeoclimatology - the latter has a prominent Comparison of all known cave bear faunas of the Alps position within the aforementioned fields of research. showed an extraordinary correlation between metrical In addition, the reconstruction of the climatic changes and morphodynamic values against the sea level of the in the Alps between the Middle Pleistocene and the cave’s entrances. The correlation varies among species. Holocene is a current key aspect of the research group. Ursus spelaeus eremus and U.ladinicus show a negative These palaeo-climatologic studies are based on material correlation between dimensions and sea-level (i.e. the that shows evidence of former climatic trends such as: higher the sea-level, the smaller the mean values of traces and sediments of glaciers, sediments of rivers and length and width in teeth and bones), while in Ursus lakes, loess and soil, decline of ice in caves, abundant ingressus, morphodynamic indices increase with the fossil cave sites. All of these are controlled by climatic higher sea-level of the cave. changes: traces of glaciers and moraines indicate former spread of glaciers and lake and turf sediments show A research project in Romania and the Ural-Caucasus effects of climatic changes in fauna and flora as well has been started. The aim is to clarify the systematic as in sinter and ice in caves. Loess and cave-sediments position of the cave bears in these regions, particularly show remains of fossil bones and artefacts which the small-sized Ursus rossicus, and the bears from preserve evidence of the environmental conditions Peştera cu Oase, and to detect possible latest survivors former humans and animals lived in. of cave bears, as well as possible refuge areas for other Late Pleistocene large mammals. Results for 2008–2009 Calcareous tuff deposits in Austria Chronology, migration and systematics of bears (R. Pavuza & co-scientists) (G. Rabeder & co-scientists) We find spectacular calcareous tuff deposits where An important step towards a better knowledge of the calciferous spring water has lost carbon dioxide because phylogeny of large bears (genus Ursus) was managed. of strong turbulences and the biological activity of The origin of brown bears was clarified by studying plants - primarily of algae and mosses. The consequence the fossil bear remains from Deutsch-Altenburg 2 and of these activities is coagulation of calcium carbonate. 4 (lower Early Pleistocene 1.3 until 1.2 million years Examination of the calcareous tuff deposits brings, on before present): they are to be deduced directly from the the one hand, information about the seasonally solid Etruscan bears (Ursus etruscus). All other bear remains fluctuation in the spring water and on the other hand, of the Early Pleistocene in Europe (and described by an indication for postglacial climate fluctuations since various names) are primitive brown bears. Based on the sedimentation rate is obviously also connected the first valid name, these have to be termed Ursus with the environmental temperatures - the number of arctos suessenbornensis. The cave bear line represented calcareous tuff deposits therefore decreases significantly by Ursus deningeri is not proved until the beginning of with the sea height (and with the temperature). Commission for Quaternary Research =<
The research of the fossil fauna (primarily gastropods) as well as the radiometric dating of the organic remains, allows for a good geochronological classification. Until now, only approx. 100 calcareous tuff deposits could be included in a database – only a small fraction of the existing deposits in Austria.
Gravettien of Krems-Wachtberg (Ch. Neugebauer Maresch & co-scientists) Continuation of the project Krems-Wachtberg (Prehis- toric Commission, AAS with support of FWF P19347): after the unique results of the explored baby burials in 2005 and 2006, the accompanying fireplace was discovered in 2007, the partial recovery in 2008 made a reconstruction of this multi-staged fireplace possible.
Intense thunderstorms as well as long negotiations with the landowner, Lower Austria and the ÖAW, hindered the excavations in 2008. In the fall of 2008 (Fig. 3) and winter 2008/2009 the work could be continued with the construction of the cellar in collaboration with the Austrian Academy of Science. Further examinations were able to take place in the shell of the cellar in the summer and fall of 2009. * / O O P /
An extensive series of wood coal samples were dated at Praehistoric land use and colonization the VERA laboratory in Vienna and this information is in the Tyrolean Alps (G. Patzelt) published in the magazine “Radiocarbon”. It seems to be The investigations of the prehistoric human land use well documented that the culture layer and the burials of the Alps near the forest line was continued in the are from a temporal horizon of about 27,000 BP. Ötztaler- and Stubaier Alps with the focal point in the area of Kühtai (mountains of Sellrain, Northern Stubaier Alps). Ten areas of slashing and burning as well as 41 fireplaces in huts of herder (in altitudes of 2,500m and more) near the ancient forest line could be dated by radiocarbon methods. Most of the samples come from the Bronze Age and from the Latène- Roman times. In medieval times and the early modern periode the “alp use” followed the consumption of firewood top down into the wodded belt.
Bibliography t Einwögerer T., M. Händel, C. Neugebauer-Mare- sch, U. Simon, P. Steier, M. Teschler-Nicola, E. M. Wild: 14C Dating of the Upper Palaeolithic Site at Krems-Wachtberg, Austria. Radiocarbon, 51, (2) 847-855, 2009. t Knapp, M., N. Rohland, J. Weinstock, G. Barysh- nikov, A. Sher, D. Nagel, G. Rabeder, R. Pinhasi, H. Schmitt, M. Hofreiter: First DNA sequences from Asian cave bear fossils reveal deep divergen- Fig. 1. Skeleton of forearm of Ursus arctos suessenbornensis from ces and complex physlogeographic patterns. Mol. @ | ; Y 8 ( Ecol., 18, (6) 1225-1238, 2009; doi: 10.1111/j.1365- 8 + Q 294X.2009.04088.x. 50 Commission for Quaternary Research
* " O V ( + O V ( ( O 0 # P X | |O @8V t Krause, J., T. Unger, A. Nocon, A.-S. Malaspi- t Pacher, M., and A. J. Stuart: Extinction chronology nas, S.-O. Kolokotronis, M. Stiller, L. Soibel- and paleoecology of the cave bear Ursus spelaeus. zon, H. Spriggs, P. H. Dear, A. W. Briggs, S. C. Boreas, 38, (2) 189-206, 2008; doi: 10.1111/j.1502- E. Bray, St.J. O‘Brien, G. Rabeder, P. Matheus, 3885.2008.00071.x. A. Cooper, M. Slatkin, S. Paabo, M. Hofreiter: t Reitner, J. M.: The potential of historic rockfalls as Mitochondrial genomes reveal an explosive radi- CRONUS-EU calibration sites: Chlorine-36 data ation of extinct and extant bears near the Mioce- from the Alps. Geophys. Res. Abstr., 10, EGU2008- ne-Pliocene boundary. BMC Evol. Biol., 8, 220, A-07189, 2008. 12 p., 2008. t Rabeder, G., I. Debeljak, M. Hofreiter, and G. t Neugebauer-Maresch Chr. (Hrsg.), Krems-Hunds- Withalm: Morphological response of cave bears steig – Mammutjägerlager der Eiszeit. Ein Nut- (Ursus spelaeus group) to high-alpine habitats. Die zungsareal paläolithischer Jäger- und Sammler(- Höhle, 59, (1-4), 59-70, 2008. innen) vor 41.000 - 27.000 Jahren. Mitt. Prähist. t Rabeder, G., M. Pacher, and G. Withalm: Early Plei- Komm. 67, Wien, 347 S., 2008. stocene bear remains from Deutsch-Altenburg (Lower t Oeggl, K., F. Mathis; J. Moser, I. Schneider, W. Austria). Mitt. Komm. Quartärforsch, 17, 1-135, 2009. Leitner, G. Tomedi, T. Stöllner, R. Krause, E. t Richards, M. P., M. Pacher, M. Stiller, J. Quilès, M. Pernicka, P. Tropper, J. Schibler, K. Nicolussi, K. Hofreiter, S. Constantin, J. Zilhao, and E. Trinkaus, Hanke: The history of mining activities in the Ty- 2008. Isotopic evidence for omnivory among European rol and adjacent areas: Impact on environment and cave bears: Late Pleistocene Ursus spelaeus from the human societies (HiMAT). Antiquity, 82, (317), Peştera cu Oase, Romania. P. Natl. Acad. Sci. USA, 105, Sept. 2008; online version. (2) 600-604, 2008; doi: 10.1073/pnas.0711063105. 51
Commission for Basic Research on Mineral Raw Materials
Head: Horst Wagner
Aims and objectives Results for 2008–2009 The aims and objectives of the commission are: In the period under review work concentrated on t Promotion of research activities finalizing the key research area “Distribution and t Coordination of research activities characteristics of facies-bound mineralizations of t Promotion of international co-operation the Carnian stage of the Eastern Alps”. Additionally t Creating an awareness in the general public of the in 2009 the new key research area “Construction importance of mineral resources minerals” was added to the program.
The commission achieves this by Further works concentrated on in the other key t Creating a multidisciplinary discussion platform research areas: (the commission) t Identifying key research areas in the field of “Element mobility and formation of mineral deposits mineral resources during the Alpine orogeny of the Eastern Alps” t Sponsoring and/or supporting research projects R. GÖD et al investigated tourmalinites which are t Publication of research results minor but regionally spread lithological elements t Collecting funding within the “Grobgneiss Complex”, a tectonic unit t Supporting exchange programmes for scientists predominantly composed of orthogneisses and t Promoting scientific development phyllonites which form part of the pre-Mesozoic t Creating public awareness of the importance polymetamorphic basement of the Eastern Alps. of minerals t Supporting the activities under the Austrian The tourmalinites form small, concordant lenses Minerals Plan and the European Raw Materials within the phyllonites displaying some few meters of Initiative. lateral extent and thicknesses hardly exceeding 0.5 m. The tourmalinites are mainly fine grained dark-black The programme of the commission is long range and rocks. Tourmaline and quartz account for 60 – 80 % focussed on identified research topics. These address by volume. Minor muscovite may occur locally. a number of open questions which were identified by Chlorite, apatite, clinozoisite and garnet band zircon the work on the “Metallogenetic Map of Austria”. Key are accessoric minerals. The tourmaline individuals are research areas are: zoned with greenish to bluish cores and dark brown t Element mobility and formation of mineral rims. Their geochemical composition corresponds to deposits during the Alpine orogeny of the Mg-rich schorls. The bulk chemistry of tourmalinites Eastern Alps is controlled by the mutual relation of tourmaline and t Mineral deposits and mineralization processes in quartz as emphasized by B2O3 contents varying between late orogenic shear zones of collision orogens 2.9 and 8.9 % by weight and resembles, discarding the t Distribution and characteristics of facies-bound elements boron and potassium, the composition of mineralizations of the Carnian stage of the the hosting phyllonites. The trace element pattern of Eastern Alps tourmalinites precludes marine environments as boron t Increasing the value of industrial minerals sources but does not allow discrimination between t Isotope map of Austria. sediments and granites as potential boron sources. 52 Commission for Basic Research on Mineral Raw Materials
Fig. 1: Photomicrographs from tourmalinite + # - 0 0 # z ( ( - + ? 0z ( composed of randomly oriented angular z - viduals in the clast indicate an older weakly preserved foliation. Note: a, b and c: plane polarized light; d: cross polarized light.
Bulk chemistries of tourmalinites are in favour of tour- ples from the Guli-Massif yielded the following sig- malinite formation by in situ metasomatism of the nificant results. Two distinct types of inclusions were hosting sediments. However, the magma parental to observed, namely numerous very small inclusions and the primary rock of the Grobgneiss does not seem to be larger more irregular shaped inclusions with no pre- a likely source of the boron to form the tourmalinites. ferred orientation within the olivine grain. In many of Therefore, the origin of boron necessary to form tour- the latter inclusions a solid black-coloured phase could malinites remains unknown. The tourmalinites and be detected. The Raman spectra produced showed their host rocks experienced two metamorphic events, significant fluorescence, which can be associated with which on the basis of regional observations are inter- graphite. Raman spectroscopy carried out on more preted to be of Permian and of late Cretaceous Age. than 100 inclusions from all samples studied revealed Zoned garnets within phyllonites reflect clearly two the presence of methane gas in all samples measured, garnet generations whereas tourmalinite hosted gar- but not in each inclusion. nets display a continuous growth which corresponds to the younger garnet generation in the phyllonites. It The presence of antigorite was identified in all samples. is therefore concluded that the tourmalinites are pre- The presence of antigorite and the lack of fluid phase Alpine and also younger than the magma parental to within the inclusions suggest, that the fluid phase the primary magmatic rocks of the “Grobgneiss”. was consumed during serpenitisation of the walls of the inclusions. Cooling of methane-rich fluids below During the last year research of the “Mobility project” 600° C should promote the precipitation of graphite, also focussed on chromite and PGE occurrences in which is indicated in the fluid inclusions studied here. ophiolitic complexes, e.g. the Kraubath ultramafic The fact that both methane and carbon are present complex of the Eastern Alps. To compare the minerali- in the inclusions implies that gas-solid inclusions had sations hosted in ultrabasites of different geodynamic not been changed at a temperature around 500° C. settings O. Thalhammer investigated chromite sam- The presence of graphite at a temperature range of ples from the Guli Massif/Siberia, the largest Alaskian- 500 to 600° C indicates low oxygen fugacities (i.e. Uralian-type complex. The chromites in the Guli Mas- QFM=-0.6 to 0.8) in these magmatic fluid systems. sif are similar to those from ophiolites with respect to In summary this fluid inclusion study provides the their occurrence and chemistry. However, Os-isotope first indication for the minimum P-T-X conditions of data show very consistent 187Os/188Os ratios, clearly in mantle pluming under metasomatic conditions and contrast with those from ophiolites. The study of sam- mineralizing processes in the Guli Massif. Commission for Basic Research on Mineral Raw Materials 53
“Isotope map of Austria” Considerable effort was devoted to the systematic collection of isotope data from Austria and neighbouring regions. To assist with the analysis and evaluation of data Rantisch and Russegger established a MS Access database linked with ArcGis 9.3.1 which contains all available data on stable isotopes 18 18 of oxygen ( OSMOW, OPDB), hydrogen (DSMOW), 34 13 sulfur ( SCDT) and carbon ( CPDB), radiogene strontium (87Sr/86Sr) and lead (208Pb/204Pb,207Pb/204P b,206Pb/204Pb) from mineral deposits in Austria. The structure of the database is closely linked to that of the metallogenetic map of Austria making it possible for the two databases to interact.
* + 8 # ( 0 ( “Walther-E.-Petrascheck-Award” ( On the occasion of the 100th anniversary of the birth of the eminent Austrian geoscientist Walther Emil Petrascheck, an academy award for young scientists “Distribution and characteristics of facies-bound miner- and engineers working in the field of minerals research alizations of the Carnian stage of the Eastern Alps” was established. Dr. Doris Reischenbacher from the Gawlick studied lead-zinc deposits in the central University of Leoben was the second recipient of region of the Northern Calcareous Alps. The present this award. In her PhD thesis she investigated the tectonic setting does not indicate a systematic pattern formation of intramontane coal basins on the example of mineralisation. However on the basis of a paleo- of the Lavanttal coal basin. The thesis was a fine geographic reconstruction of the region, which is demonstration of the benefits of an interdisciplinary representative of the situation prior to the lateral research approach. tectonic extrusion, for example on the basis the Oligocene paleo-geography, it is observed that the Creating public awareness of the activities of the commission lead-zinc deposits occur within a narrow band which On the occasion of the 30th anniversary of the corresponds to the Lower- as well as Upper-Tyrolic commission a symposium with the title “Mineral nappe blocks. These results suggest that prospecting resources - A challenge for research and minerals policy” strategies should be based on the paleo-geographic was held on April 15, 2008. The symposium attracted reconstruction of this region whilst giving also considerable interested and highlighted the dependency consideration to the multi-phase tectonic situation. of modern society on minerals.
Fig. 3: Locations of data documented in the Austrian isotope data base. 54 Commission for Basic Research on Mineral Raw Materials
In addition members of the commission are actively of Minerals Research, Wien, 2009. engaged in Austrian and European minerals policy t Paar, W.H., et.al.: Daliranite, PbHgAs2Se, formulation. a new sulphosalt from the Zarshouran Au- As deposit, Tabak region, Iran. Mineralogical Bibliography Mag., 73, (5) 871-881, 2009; doi: 10.1180/min- t Göd, R., and G. Heiss: On the geochemistry and mag.2009.073.5.871. mineralogy of phyllite hosted tourmalinites - Easten t Unterweissacher, T., et al.: Geologie der Alps. Mitt. Österr. Miner. Ges., 155, p. 61, 2009. Magnesitlagerstätten im Raum Tavsanli (Türkei). t Thalhammer, O. A. R, and J. P. Pink: Fluid inclu- Mitt. naturwiss. Ver. Steiermark, 139-149, 2009. sions in chromite and platinum deposits. Final Re- t Kucha, H., and J. G. Raith: Gold-oxysulphides port, Commission on Fundamentals of Minerals in copper deposits of the Greywacke Zone, Aus- Research, Wien, 2009. tria: A mineral chemical and infrared fluid inclu- t Gawlick, H.-J.: Temperaturüberprägungskartierung sion study. Ore Geol. Rev., 35, 87-100, 2009; doi: der Karbonate des Karn im Mittelabschnitt 10.1016/j.oregeorev.2008.10.004. der Nördlichen Kalkalpen – Möglichkeiten zur t Nishimura, C., J. Matsuda, and G. Kurat: Noble Abschätzung der Genese/Verteilung der Blei/ gas content and isotope abundances in phases of Zink Vererzungen. Final report, Commission on the Saint-Aubin (UNGR) iron meteorite. Meteorit. Fundamentals of Minerals Research, Wien, 2009. Planet. Sci., 43, (8) 1333-1350, 2008; doi: 10.1111/ t Gawlick, H.-J., and F. Schlagenweit, F: Revi- j.1945-5100.2008.tb00701.x. sion of the Tressenstein Limestone: reinterpre- t Hurai, V.; O. Lexa, K. Schulmann, R. Montigny, W. tation of the late Late Jurassic to ? Early Creta- Prochaska, W. Frank, P. Konecny, J. Kral, R. Thom- ceous platform –basin transition of the Plassen as, M. Chovan: Mobilization of ore fluids during Al- Carbonate Platform (Austria, Northern Cal- pine metamorphism: evidence from hydrothermal careous Alps). J. Alpine Geol., 51, 1-30, 2009. veins in the Variscan basement of Western Carpathi- t Rantitsch, R. und B. Russegger: Isotopen-Daten- ans, Slovakia. Geofluids, 8 , 181 – 207, 2008; doi: bank. Final report, Commission on Fundamentals 10.1111/j.1468-8123.2008.00216.x 55
Mathematics, Simulation and Metrology
Institute for Integrated Sensor Systems
Head: Thilo Sauter
Aims and Functions mechanisms of various microcantilevers. The focus The institute was established in 2004, its basic idea was on a concise analytical and computer-numerical being to combine in a multidisciplinary way those modelling of all relevant physical effects dominating research fields that are essential for modern integrated in individual regions of the ambient pressure. These sensor systems: sensor technology, microelectronics, studies enabled the improvement of existing analytical and communication technology. The focus is on the models for the pressure dependence of the quality structural aspects of the investigated sensor systems, thus factor and resonance frequency. The developed models the weight of the three complementary fields can vary were verified using a specially developed vacuum from case to case. Adequate modelling plays a central chamber and a microsystem analyzer and now allow a role in both the analysis and design of sensor systems, precise prediction of the oscillatory behaviour over an and the methods employed range from the investigation extraordinarily wide pressure range. of analytical models to numerical analyses. For flow measurements, a new class of thermal flow The integration aspects the institute derives its name sensors was developed and investigated. The sensors from can be manifold. They comprise circuitry (with are based on the self-heating effect of microthermis- respect to miniaturization of the sensor or the com- tors embedded in a common thin membrane to achieve ponents relevant for signal processing) as well as thermal interaction enabling flow magnitude measure- functional aspects (mechanisms to increase fault tol- ments and flow direction recognition with very low erance, but also to combine various sensor principles power budget. An impressive demonstration of such in order to improve accuracy, measurement range, or sensors was achieved by the implementation of a com- robustness) or system-related aspects (in terms of con- puter mouse that exploits the air circulation within the necting sensors to higher-level systems). The institute thin air gap underneath the mouse body. has particular expertise in the areas of miniaturised thermal sensors, resonant micromechanical sensors, As a milestone towards fully implantable hearing aids optimized system architectures and signal processing in conjunction with cochlear stimulator prostheses, mi- for integrated sensors as well as synchronisation and cromechanical sensors were investigated enabling the security aspects in sensor networks. detection of tiny vibrations of ossicles inside the middle ear. This application demands a very challenging combi- Co-operation with external partners is of high sig- nation of sensitivity (5·10-13 mHz-1/2), sensor dimensions nificance in the definition and implementation of (<2 mm side length), weight (<25 mg), and power con- research projects. The institute collaborates with other sumption (<100 μW). We designed and investigated ca- research institutes (both university and non-univer- pacitive transducers that were produced from microma- sity) and industrial partners alike. Concrete projects chined Silicon in cooperation with a technology partner. comprise problems from automation, medical tech- Measurements showed that the first prototypes already nology, or test and measurement technology. largely fulfil the requirements. The very encouraging experimental results are complemented with a theoreti- Results for 2008–2009 cal study of the margin for further improvement of the Exploiting previous experience with resonant characteristics and for the first time demonstrate the magnetic field sensors we investigated the damping technological feasibility of the sensor concept. 56 Institute for Integrated Sensor Systems
expected for low-volume individualized products and single item production, since each order is control- led by an individual agent tracking and planning the execution of its order. For the RFIDs, a security sys- tem using asymmetric cryptography was developed to prevent attacks on the system. Tests showed that the developed agent platform by far exceeds the perform- ance of state-of-the-art platforms and still maintains full compatibility with current standards.
Bibliography t Beigelbeck, R., F. Kohl, F. Keplinger, B. Jakoby: Closed-form 3D-analysis of membrane-based mi- cromachined sensors measuring the thermal prop- erties of liquids, in: Proceedings EUROSENSORS XXII Conference, Dresden, 7.-10.9.2008, Paper-Nr. Fig. 1: Investigation of a magnetically actuated microcantilever in a vacuum chamber underneath a microsystem analyser. The Helmholtz 593, pp. 76-79, 2008. t Bratukhin, A.: Production order life cycle in agent- based distributed manufacturing, in: Proceedings 13th IEEE International Conference on Emerging The long-term research on fault tolerant clock syn- Technologies and Factory Automation, Hamburg, chronization in heterogeneous networks could be Germany, Sep. 2008, pp. 1496-1503, 2008. successfully finished. For the first time, a state-of- t Cerimovic, S., A. Talic, F. Kohl, R. Beigelbeck, the-art fault-tolerant clock synchronization solution J. Schalko, A. Jachimowicz: Micromachined flow according to the PTP standard was investigated and sensors enabling electrocalorimetric and TOF proposed for standardization. Another focus was the transduction. Procedia Chemistry, 1, (1) 132-135, analysis and development of security mechanisms for 2009; doi: 10.1016/j.proche.2009.07.033. distributed synchronization. As a by-product of the t Cerimovic, S., A. Talic, T. Sauter, F. Kohl, R. fundamental research, it was possible to find a novel Beigelbeck, J. Schalko, A. Jachimowicz: A novel model for crystal oscillators describing their statisti- thermal transduction method for sub-mW flow cal behavior in a very high level of detail. This model facilitates the understanding of systems aiming at highly accurate clock synchronization. On the other hand, simulation of large-scale networks comprising such oscillators becomes more efficient while at the same time increasing the simulation accuracy. Anoth- er highlight was a new world record in the synchro- nization accuracy of two computer clocks over Ether- net. For the first time it was possible to synchronize two nodes below 700 ps with respect to each other. This result from the laboratory will be used in further projects such as large-scale clock synchronization as in the Large Hadron Collider at CERN.
Within the focal area of Security and Integrable Net- works, we developed and evaluated an agent runt- ime environment scalable enough to run on an ac- tive RFID (Radio Frequency Identification) tag. This runtime environment is a basis for future intelligent Fig. 2: Micromechanical vibration sensor for an implantable middle manufacturing execution systems. The combination ear microphone. The insets show the partially movable electrodes of of software agents and RFIDs allows that the agent is a capacitor whose capacitance changes because of the vibrations, directly attached to the product. Special benefits are which yields the output signal. Institute for Integrated Sensor Systems 57
Fig. 3: Evaluation platform for high-accuracy clock synchronization in wired and wireless computer networks.
sensors, in: Proceedings IEEE Conference on Sensors, CRC Pre s s, B oc a R aton, Florid a , USA , 1160 p., 20 09. Christchurch, New Zealand, 25.-28.10.2009, pp. t Schalko, J., R. Beigelbeck, F. Kohl, A. Jachimo- 1325-1328, 2009. wicz, F. Keplinger, M. Opitz: Novel Carbon Na- t Gaderer, G., A. Nagy, P. Loschmidt, N. Kerö: A notube Fabric Based Membranes, in: Proceedings Novel, High resolution oscillator model for DES EUROSENSORS XXII Conference, Dresden, 7.- systems, in: Proceedings 2008 IEEE International 10.9.2008, Paper-Nr. 360, pp. 660–663, 2008. Frequency Control Symposium, pp. 178-183, 2008; t Treytl, A., B. Hirschler: Security Flaws and Work- doi: 10.1109/FREQ.2008.4622984. arounds for IEEE 1588 (Transparent) Clocks”, in: t Loschmidt, P., R. Exel, A. Nagy, G. Gaderer: Lim- Proceedings 2009 IEEE Symposium on Precision its of Synchronization Accuracy Using Hardware Clock Synchronization for Measurement, Control Support in IEEE 1588, in: Proceedings 2008 IEEE and Communication, 6 p., 2009; doi: 10.1109/ Symposium on Precision Clock Synchronization for ISPCS.2009.5340204. Measurement, Control and Communication, pp. 12- t Treytl, A., W. Spenger, B. Riaz: Secure Agent 16, 2008; doi: 10.1109/ISPCS.2008.4659205. Platform for Active RFID, in: Proceedings 2008 t Sauter, T.: Fieldbus systems – embedded networks IEEE Conference on Emerging Technologies and for automation, in: Networked Embedded Systems Factory Automation, pp. 492-495, 2008; doi: Handbook, R. Zurawski (Ed.), pp. 20-1 – 20-64, 10.1109/ETFA.2008.4638444. W>
Acoustics Research Institute
Head: Werner Deutsch
Aims and Functions Psychoacoustics The institute’s individual fields of research are The description of traffic noise in terms of psychoacoustic complementary. Research in hearing needs the accurate parameters including timbre, and in addition to standard measurement and simulation of sound sources and noise criteria, improves the precision of noise abatement sound fields, as well as the study of auditory functions. and noise reduction measures considerably. The institute’s software NOIDESc introduces a classification scheme of The institute’s policy follows the goals of application- timbre, based on the principal component analysis of third- oriented basic research. For this reason, the scientific octave frequency bands. The new project sysBahnLärm work aims at practical application, whilst the theoretical uses the institute’s Noise Monitoring System to collect and background is evaluated using acoustic measurements, analyse sound data from the newly introduced Railjet psychoacoustic experiments, and real life sound trains, as well as additional freight train pass-by events. material. The concept guarantees progress in theory as well as scientific results in applied research. Audiological Acoustics Substantial progress in auditory localization with Results for 2008–2009 cochlear implants reached the application level with the issue of a patent on a novel stimulation Computational Acoustics strategy (a spin-off from project FWF P18401-B15). Numerical simulations in acoustics, and Cooperation with the Eaton-Peabody Lab at Harvard- computational models of hearing were applied to MIT Medical School will deal with the evaluation improve applications such as hearing with cochlear and neurophysiological proof of the Viennese implants and environmental noise reduction. In a experiment results. A cooperative research project on recently accepted FFG-ÖBB project sysBahnLärm, auditory localization, bringing together partners in accurate noise source localisation, such as ascertaining neurophysiology, neural models and psychoacoustics, whether noise arises from the wheel or the track is intended to be submitted during 2010. (Fig. 1), will be performed by a specific adaption of the microphone array technique (64 channels). The Acoustic Phonetics institute’s microphone array system is programmable In the area of public security, the almost worldwide and can also be used for acoustic holography. ongoing discussion on the validity of automatic speaker recognition systems in forensic cases, as opposed to The solution of the boundary integral equation for the traditional phonetic expertise, is being addressed in study of railway generated Vibrations in Anisotropic Media cooperation with the German Federal Criminal Police (ORTHOSOL) was improved, especially with respect Office (BKA, Wiesbaden). The project line is funded by to the generation of the stiffness matrix, which includes an EC-grant and recognized by the Austrian BK. Since the integrals with singular integrands. In addition, the Fast software simulation of the main functions of automatic Mulitpole Boundary Element Method (FMBEM) was speaker recognition systems (using S-TOOLS-STx) has combined with the Finite Element Method (FEM), been completed, an international evaluation test can enabling the joint simulation of structures and acoustic be started in the framework of the Working Group for sources. Future work is planned to deal with the non- Forensic Speech and Audio Analysis (FSAAWG) of the linearities of high amplitude signals. European Network of Forensic Science Institutes (ENFSI). Acoustics Research Institute W<
Fig. 1: Accurate noise source localisation in the range of the wheel- * + ( % # 0 # # - X= ! ( ) Y
Mathematics in Acoustics and Signal Processing re-synthesis, descriptive statistics etc. S_TOOLS-STx The working group deals with time-frequency analysis, adheres to a strict cumulative concept to guarantee reuse, time variant digital filters, and sound synthesis. The scalability and sustainability. It is designed to serve the WWTF-project Frame Multipliers: Theory and Acoustic inexperienced user by providing a large set of functions Applications investigates the theoretical aspects of frame called up via dialogs and menu windows. In addition, multipliers, as operators: k k k, it allows expert users to process large data sets in batch where the analysis coefficients are multiplied mode. S_TOOLS-STx is programmable in a macro by a fixed symbol before re-synthesis (with k). First language, enabling programmers to write their own results were obtained in the theory of weighted frames extensions, such as new signal processing algorithms, and the new formulation of the psychoacoustic Model perceptive models or sound database applications. The of Irrelevance, based on simultaneous masking. S_TOOLS-STx software development is managed in a professional version control system and includes an Industrial Co-operations HTML based help system. Licenses are free for students External project contracts extend from automotive audio (3-month period) and for non-profit academic projects. and the simulation of sound fields in car cabins to the application of wavelet transformations and members Bibliography of the Cohen class for transient signals in mechanical t Balazs, P.: Matrix representation of operators using engineering. The appropriate choice of the transformation frames. Sampl. Theory Signal Image Process., 7, (1) parameters for typical applications is described in a 39-54, 2008. handbook. A feasibility study for the sound quality t Balazs, P.: Hilbert-Schmidt operators and frames design of electrical switch clicks (Sound Quality Design) - classification, approximation by multipliers was completed by applying the wavelet implementation and algorithms. Int. J. Wavelets Multiresolution in S_TOOLS-STx. Co-operation with industry partners Inf. Process., 6, (2) 315-330, 2008; doi: 10.1142/ from different countries creates important synergies. S0219691308002379. Usually the scientific methods and procedures provided t Becker, T., M. Jessen, and C. Grigoras: Speaker by the institute are evaluated immediately and feedback verification based on formants using Gaussian by qualified development engineers is given. mixture models, in: Proceedings NAG/DAGA 2009 International Conference on Acoustics, Marinus M. Software Development Boone (Ed.), pp. 1640-1643, 2009. The institute’s software system S_TOOLS-STx is the t Chen Z.-S., H. Waubke and W. Kreuzer: A formula- back-bone of all signal processing tasks in the above tion of the fast multipole boundary element method projects. Currently, in version 3.9.0, it provides almost (FMBEM) for acoustic radiation and scattering from all conventional signal processing functions necessary to three-dimensional structures. J. Comput. Acoust., 16, (2) perform time-frequency analysis, digital filtering, signal 303-320, 2008; doi: 10.1142/S0218396X08003725. 60 Acoustics Research Institute t Kreuzer, W., P. Majdak, and Z.-S. Chen: Fast t Laback B. and P. Majdak: Binaural Stimulation in Neu- multipole boundary element method to calculate ral Auditory Prostheses or Hearing Aids. E U a n d U S p a t- head-related transfer functions for a wide fre- ent WO/2008/155123. Appl. Date: 19.06.08, 2008. quency range. J. Acoust. Soc. Am., 126, 1280-1290, t Majdak, P. and B. Laback: Effects of center frequen- 2009; doi: 10.1121/1.3177264. cy and rate on the sensitivity to interaural delay in t Laback B. and P. Majdak: Binaural jitter improves high-frequency click trains. J. Acoust. Soc. Am., 125, interaural time-difference sensitivity of cochlear (6) 3902-3913, 2009; doi: 10.1121/1.3120413. implantees at high pulse rates. P. Natl. Acad. t Moosmüller, S.: Vowels in Standard Austrian German. Sci. USA, 105, (2) 814-817, 2008; doi: 10.1073/ An Acoustic-Phonetic and Phonological Analysis, pnas.0709199105. Habilitationsschrift, Universität Wien, 271 S., 2007. 61
Johann Radon Institute for Computational and Applied Mathematics
Head: Heinz Engl
Aims and Functions In 2008, several members of RICAM (including group During 2008 and 2009, the Johann Radon Institute for leader Prof. Albrecher) accepted offers for professorships. Computational and Applied Mathematics (RICAM) The necessary partial rebuilding of the Institute had to employed between 50 and 60 scientists, about half be postponed to 2010 for budgetary reasons. of them being financed from external funds (mainly FWF); they joined RICAM coming from 16 different Results for 2008–2009 countries. RICAM cooperates with the Johannes Kepler Universität Linz in educating PhD-students and, as far Computational Methods for Direct Field Problems as “real world” applications are concerned, with the (Ulrich Langer) Industrial Mathematics Competence Center. RICAM The research of the Group has focused on the does applications-oriented basic research currently in development, analysis and implementation of eight groups led by the senior scientists mentioned computational methods for Partial Differential t Computational Methods for Direct Field Problems Equations with the focus on the following topics: (Ulrich Langer) t Algebraic multigrid methods for large-scale techni- t Inverse Problems (Heinz Engl) cal problems and problems in life sciences (on this t Financial Mathematics (Hansjörg Albrecher) topic a monograph has been published recently). t Symbolic Computation (Josef Schicho) t High-order finite element methods with applica- t Optimization and Optimal Control (Karl Kunisch) tion to solid and fluid mechanical as well as elec- t Analysis of Partial Differential Equations (Peter trical engineering problems. Markowich, Massimo Fornasier) t Mathematical Imaging (Otmar Scherzer) The latter topic is partly connected with the START t Mathematical Methods in Molecular and Systems project “hp-FEM” led by Joachim Schöberl. The Biology (Philipp Kügler, Christian Schmeiser). START project was supported by the Austrian Science Fund under the grant Y192 until 2008. While the last mentioned group is located at the Vien- na BioCenter, all other groups are based on the campus Furthermore, new Domain Decomposition Methods of Johannes Kepler University in Linz. (DDM) have been developed. Fig. 1 shows the An important aspect is the cooperation between these simulation of an electrical machine by means of the groups, e.g., connecting analytical and numerical with new DDM within a project with the ACCM in Linz. symbolic and algebraic methods. Among the application areas considered are mathematical methods in imaging, Inverse Problems (Heinz Engl) numerical and optimization methods for solid and fluid The group is active in the mathematical theory and mechanics and mathematical modelling and algorithm numerics of regularization methods, and in applying development for molecular any systems biology. inverse problems techniques to science, engineering and While in 2008, a “Special Semester on Mathematical finance. Recent achievements concern the development Finance” with many international participants could of regularization methods for the stable reconstruction be held, the Special Semester on Inverse Problems of sparse solutions including a convergence theory, planned for 2009 had to be held in a severely reduced which is complicated due to the non-convexity of the form due to budgetary constraints. penalty term. Significant progress has been made towards 62 Johann Radon Institute for Computational and Applied Mathematics identification of complex objects from measurements of far fields of acoustic and electromagnetic waves and in a theory-based further development of Bayesian inversion methods. The group is a member of the EU- consortium “DIAdvisor” aimed at the improvement of diabetes therapy and develops, in this context, methods for the prediction of blood glucose levels.
In October 2009, the project “Mathematical meth- ods and Algorithms for E-ELT Adaptive Optics” con- tracted by ESO was started. Its main aim is the de- velopment of algorithms for reconstrucing incoming wavefronts and for determining mirror deformation for image improvement.
Financial Mathematics (Hansjörg Albrecher) New characterizations for optimal dividend payout strategies in insurance portfolios led to a remarkable extension of solvable model classes, with the ability to Fig. 1: Simulation of electrical machines with DDM inlcude transaction costs in the model as well. Hitting time identities for stochastic processes refracted at their running maximum could be extended to a general fluids, and semi-smooth Newton methods for optimal Lévy setup. Efficient calibration of complex finance control for elliptic systems with pointwise polygonal market models could further be developed by suitable state constraints were investigated. We accomplished application of Tikhonov regularization. Several new an error analysis for the P1 nonconform finite element theoretical results with applications in cryptography, for the penalized Navier-Stokes equations. wireless communication and coding theory were obtained, including a theoretical justification for the Further feasible direction and direct search methods hardness of the double discrete logarithm problem were investigated for non-linear bi-level programming and a result that every good quaternary sequence problems with the convex lower level problems. corresponds to two good uncorrelated binary sequences. Due to the fact the Prof. Albrecher has left, Analysis of Partial Differential Equations (Peter Marko- the group will be closed at least temporarily. wich, Massimo Fornasier) The group focussed on the following topics. Symbolic Computation (Josef Schicho) t Variational methods and high-order PDEs for The balancing problem for linkages is to find image processing. Significant results have been distributions of weights on the movable parts such that achieved in the mathematical restoration, recolo- the sum of the forces exerted by movements vanishes. rization and retouching, of damaged or destroyed Such mechanisms are useful in applications in space frescoes. and in optics. Using the symbolic method of toric t Non-linear water waves: existence theory of genera- polynom division, we found algebraic conditions for lized solutions and stability results in the presence the balancing of four bar mechanisms and could give of shock on the gradient of the solution. the complete classification of the all solutions. t Kinetic equations and collective behavior mode- ling: analysis of existence and stability of solutions, Optimization and Optimal Control (Karl Kunisch) large time behavior, pattern formation, and nume- The research focus was put on optimal control with rical simulation. PDE models for swarming of ani- partial differential equations as constraints. We mals, and aggregation-motion of biological cells. successfully started the numerical verification of optimality conditions for optimal control problems. The In the period 2008-2009 the group has been awarded investigation of flow control problems was continued for with an Outstanding Thesis Award, a START-Prize, non-Newtonian flow and boundary control with state and the Boelpaepe-Prize of the Academy of Science of constraints. An optimal control problem for two phase Belgium (the last two for M. Fornasier). Johann Radon Institute for Computational and Applied Mathematics 63
* + | ; R | ) Fig. 3: Recolorization of the frescoes by means of mathematical methods
Mathematical Imaging (Otmar Scherzer) were led with representatives of IMP, IMBA and the The group developed a general framework for the University of Vienna (Institute for Pharmacology mathematical analysis of non-local functionals, which and Toxicology, Department for Molecular Systems in particular have been successfully implemented Biology). Mathematical methods from the areas of for data analysis. One of these methods is non-local inverse problems and partial differential equations were patch-based filtering. Jointly with INRIA Rennes and developed successfully for answering questions related the Institute Curie Paris, this method was applied to transport through cell membranes, biochemical for denoising biological data. In addition, via non- reaction networks, voltage-gated ion channels and convex regularization (one of our ongoing research chemotaxis. Furthermore, the group is responsible for topics), a relationship between the optical flow two WWTF projects on stress hormone regularization and level-set methods has been established. and cytoskeleton dynamics. In the long-term project of developing practically useable methods for photo-acoustic tomography, real Bibliography biological samples were successfully analyzed. t Kraus, J. and S. Margenov: Robust Algebraic Multilevel Methods and Algorithms. Radon Series Mathematical Methods in Molecular and Systems Biol- on Computational and Applied Mathematics 5, de ogy (Philipp Kügler, Christian Schmeiser) Gruyter, Berlin, 246 p., 2009. The group was founded at the beginning of 2009 t Lazarov, R., S. Repin, and S. Tomar: Functional and is currently still in the phase of recruiting and a posteriori error estimates for discontinuous organizational integration into the Vienna BioCenter. Galerkin approximations of elliptic problems. Discussions about interdisciplinary cooperation Numer. Meth. Part. D. E., 25, 952–971, 2009; doi: 10.1002/num.20386. t Cao, H., M. V. Klibanov, and S. V. Pereverzev: A Carleman estimate and the balancing principle in the quasi-reversibility method for solving the Cauchy problem for the Laplace equation. Inverse Probl., 25, 035005, 21 p., 2009; doi: 10.1088/0266-5611/25/3/035005. t Albrecher H. and D. Kortschak: On ruin probability and aggregate claim representations for Pareto claim size distributions. Insur. Math. Econ., 45, 362-373, 2009; doi: 10.1016/j.insmatheco.2009.08.005. t Fornasier, M., R. Ramlau, and G. Teschke: The application of joint sparsity and total variation * =+ D V # minimization algorithms to a real-life art restoration 64 Johann Radon Institute for Computational and Applied Mathematics
problem. Adv. Comput. Math., 31, 157-184, 2009; t Engl, H. W., C. Flamm, P. Kügler, J. Lu, S. Müller, doi: 10.1007/s10444-008-9103-6. P. Schuster: Inverse Problems in Systems Biology. t Gosselin, C., B. Moore, and J. Schicho: Dynamic Inverse Probl., 25, 123014, 51 p., 2009; doi: balancing of planar mechanisms using toric 10.1088/0266-5611/25/12/123014. geometry. J. Symb. Comp., 44, 1346-1358, 2009; t Griesse, R., and K. Kunisch: A semi-smooth doi: 10.1016/j.jsc.2008.05.007. Newton method for solving elliptic equations with t Kügler, P., E. Gaubitzer, and S. Müller: Parameter gradient constraints. ESAIM-Math. Model. Num., identification for chemical reaction systems using 43, 209-238, 2009; doi: 10.1051/m2an:2008049. sparsity enforcing regularization - a case study for t Scherzer, O., M. Grasmair, H. Grossauer, M. the Chlorite - Iodide reaction. J. Phys. Chem. A, Haltmeier, F. Lenzen: Variational Methods in 113, 2775-2785, 2009; doi: 10.1021/jp808792u. Imaging, Springer, New York, 322 p., 2009. 65