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Featured Products for Cellular Neuroscience Research

https://neuros.creative-biolabs.com TO PROMOTE NEUROSCIENCE RESEARCH

TO BUILD A HEALTHIER TOMORROW 01 Overview

Our Mission Cellular Neuroscience Tools for Cellular Neuroscience Research

02 GPCR Stable Cell Line

Introduction of GPCRs Classification of GPCRs GPCR Stable Cell Line Products Applications & Features

03 Sensory Neuron Culture

Introduction of Sensory Neuron Types & Functions of Sensory Neuron NeuSenso™ Media Products

04 Calcium & Glutamate Signaling

Genetically Encoded Calcium Indicators Genetically Encoded Glutamate Indicators Ready to Use AAV Biosensors

03 Everything we do is rooted in neuroscience.

Creative Biolabs is a biotechnology company that focuses on discovering, developing, manufacturing and delivering innovative, high-quality scientific tools for world-wide neuroscientists. Particularly, our vision is to enable our customers to achieve outstanding success and breakthroughs in the principles of serious neurology disorders such as Amyotrophic Lateral Sclerosis (ALS, also known as Lou Gehrig’s disease), Multiple Sclerosis (MS) and Parkinson’s Disease (PD). We thereby make improvements in life possible.

图片来自截图： https biolabs.com/support/brochure/ne ural com.pdf

04 01 Overview Cellular neuroscience is the study of neurons at the cellular level, including cell morphology and physiological properties. It examines various types of neurons, the function of different neurons, and the interaction between neurons.

There are over 8 billion neurons in the human brain, and they are diverse with respect to morphology and functions. The function of neurons is to receive, propagate and transmit electrochemical impulses. The study of neurons at the cellular level provides insight into how neurons carry out their function, the mechanism of signal transductions and the primary cause of neurological diseases.

GPCR Stable Cell Lines for Drug Discovery

GPCRs are involved in a wide variety of physiological processes and diseases. The pharmaceutical industry uses GPCR stable cell lines in target discovery, target validation, assay development, and compound screening. They are also used in academia to evaluate the mechanisms of biological processes.

Genetically Encoded Calcium Indicators for Calcium Imaging

In neurons, electrical activity is always accompanied by an influx of Ca2+ ions. Thus, calcium imaging can be used to visualize and decode the electrical activity in hundreds of neurons in cell culture or living animals, which has made it possible to dissect the function of neuronal circuits, as well as to investigate the roles that calcium ions have in functioning neurons.

Neural Cell Culture System

Neuronal cultures are indispensable in the fields of neuroscience and pharmacology. The ability to produce in vitro cultures of neuronal cells has been fundamental for advancing our understanding of the functions of the nervous system. We specialize in providing the highest quality media supplements, adhesion substrates, verification antibodies, and neuromodulators for neuronal cell culture research.

05 01 Overview G -coupled Receptors (GPCRs), also konwn as 7- transmembrane receptors (7-TM receptors), are the largest family of membrane receptors with nearly 1,000 presented in the . They are found only in eukaryotes (e.g., yeast, plants and animals), and can be activated by , , , odors, etc. Being involved in diseases such as cancers, immunological disorders, metabolic disorders, cardiovascular diseases, and mental illnesses, GPCRs are an important drug targets and estimated to be targets of 50% of drugs currently on the market.

There are two signaling transduction pathways involving GPCRs: the cAMP signaling pathway and the phosphatidylinositol signaling pathway. The mechanism of the signaling pathways includes the activation of GPCR by an external signal in the form of a ligand, which creates a conformational change in the , thus, causing activation of a (guanine nucleotide-binding ).

Class Receptor Family Name Type of Ligand

Class A (or 1) -like receptors , , , and Light

Class B (or 2) family receptors Hormone (e.g., secretin and )

Class C (or 3) Metabotropic glutamate/ receptors Glutamate

Class D (or 4) Fungal mating pheromone receptors Mating factors

Class E (or 5) Cyclic AMP receptors cAMP

Class F (or 6) / receptors Wnt proteins/Hedgehog (Hh)

One of the great features of GPCRs is that they are druggable. Despite that, only a small percentage of the known and verified GPCRs have been targeted for therapeutics. Hence, there remains enormous scope for researchers to delineate the numerous roles of GPCRs in basic physiology and pathophysiology, and to thereby understand and develop new treatments for disease.

06 02 GPCR Stable Cell Line Customer-focus, Research-driven GPCR Stable Cell Line Engineering Service Stable cell lines play an important role in the treatment of many diseases, especially neurological disorders. As a trusted product and service provider, Creative Biolabs is dedicated to offering a wide spectrum of GPCR stable cell lines for various applications including analysis of different protein expressions at the cell surface. With extensive experience and advanced technologies, we have gained a reputation among our customers worldwide for accomplishing numerous challenging projects in the research field of neuroscience.

Besides, Creative Biolabs has developed a well-established codon optimization and vector design platform to improve the performance of our GPCR stable cell lines. All the products from us meet the requirement of authority and the data provided by us is highly accurate and reproducible. With us, you are guaranteed with cost-effective products and services, and satisfactory progress of your project.

• Identifying ligands of GPCR and studying the mechanism of the ligand-receptor interaction.

• Screening for or antagonists for GPCRs.

• High through-put compound screening for related neurological diseases.

• Broad Receptor Coverage: Over 60 GPCR families with over 130 members.

• Genetic Stability: No significant changes for at least 10 passages.

• No Contamination: No contamination from neither other cell lines, or mycoplasma.

07 02 GPCR Stable Cell Line Family Receptor Product Name Cat.No.

5-HT1A/HTR1A Human Recombinant 5-HT1A Serotonin Receptor Stable Cell Line NCL20120001CR

5-HT2A/HTR2A Human Recombinant 5-HT2A Serotonin Receptor Stable Cell Line NCL20120002CR 5-Hydroxytryptamine 5-HT2B/HTR2B Human Recombinant 5-HT2B Serotonin Receptor Stable Cell Line NCL20120003CR receptor 5-HT2C/HTR2C Human Recombinant 5-HT2C Serotonin Receptor Stable Cell Line NCL20120004CR

HTR4 Human Recombinant 5-HT4 Serotonin Receptor Stable Cell Line NCL20120006CR

Human Recombinant Muscarinic M1 Stable Cell M1/CHRM1 NCL20120007CR Line

Human Recombinant Muscarinic Acetylcholine Receptor M2 Stable Cell M2/CHRM2 NCL20120008CR Line

Acetylcholine receptor Human Recombinant Muscarinic Acetylcholine Receptor M3 Stable Cell M3/CHRM3 NCL20120009CR (muscarinic) Line

Human Recombinant Muscarinic Acetylcholine Receptor M4 Stable Cell M4/CHRM4 NCL20120010CR Line

Human Recombinant Muscarinic Acetylcholine Receptor M5 Stable Cell M5/CHRM5 NCL20120011CR Line

ADORA1 Human Recombinant Stable Cell Line NCL20120012CR

ADORA2a Human Recombinant Stable Cell Line NCL20120014CR

Adenosine receptor ADORA2a Mouse Recombinant Adenosine A2A Receptor Stable Cell Line NCL20120015CR

ADORA2B Human Recombinant Stable Cell Line NCL20120016CR

ADORA3 Human Recombinant Stable Cell Line NCL20120017CR

ADRA1B Human Recombinant ADRA1B Adrenoceptors Stable Cell Line NCL20120018CR

ADRA1D Human Recombinant ADRA1D Adrenoceptors Stable Cell Line NCL20120019CR

ADRA2A Human Recombinant ADRA2A Adrenoceptors Stable Cell Line NCL20120020CR

Adrenoceptors ADRA2B Human Recombinant ADRA2B Adrenoceptors Stable Cell Line NCL20120021CR

ADRA2C Human Recombinant ADRA2C Adrenoceptors Stable Cell Line NCL20120022CR

ADRB1 Human Recombinant ADRB1 Adrenoceptors Stable Cell Line NCL20120023CR

ADRA1A Human Recombinant ADRA1A Adrenoceptors Stable Cell Line NCL20120024CR

AT1 Human Recombinant Angiotensin Receptor 1 Stable Cell Line NCL20120025CR Angiotensin receptor AT1 Human Recombinant Angiotensin Receptor 1 Stable Cell Line NCL20120026CR

Apelin receptor AGTRL1 Human Recombinant Receptor Stable Cell Line NCL20120027CR

Bile acid receptor GPBAR1 Human Recombinant Bile Acid Receptor 1 Stable Cell Line NCL20120028CR

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08 02 GPCR Stable Cell Line Family Receptor Product Name Cat.No.

BB1 Human Recombinant BB1 Receptor Stable Cell Line NCL20120029CR

BB2/GRPR Human Recombinant BB2 Stable Cell Line NCL20120030CR Bombesin receptor BB3/BRS3 Human Recombinant BB3 Bombesin Receptor Stable Cell Line NCL20120031CR

Bradykinin receptor B2 Human Recombinant B2 Receptor Stable Cell Line NCL20120032CR

AMY1 Human CHO-K1/Gα15/AMY1 Stable Cell Line NCL20120033CR

Calcitonin AMY2 Human CHO-K1/Gα15/AMY2 Stable Cell Line NCL20120034CR

AMY3 Human CHO-K1/Gα15/AMY3 Stable Cell Line NCL20120035CR

Calcitonin receptor CALCR/CT Human Recombinant Stable Cell Line NCL20120036CR

CNR1/CB1 Human Recombinant 1 Stable Cell Line NCL20120037CR Cannabinoid receptor CB2 Human Recombinant Cannabinoid Receptor 2 Stable Cell Line NCL20120038CR

Chemerin receptor CMKLR1 Human Recombinant -like 1 Stable Cell Line NCL20120039CR

CCR1 Human chemokine (C-C motif) receptor 1 (CCR1) Stable Cell Line NCL20120040CR

CCR5 Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line NCL20120041CR

CXCR1/IL8RA Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line NCL20120042CR Chemokine receptor CXCR3 Human chemokine (C-X-C motif) receptor 3 (CXCR3A) Stable Cell Line NCL20120043CR

CXCR4 Human Recombinant CXCR4 Receptor Stable Cell Line NCL20120044CR

CXCR5/BLR1 Human chemokine (C-X-C motif) receptor 5 (CXCR5) Stable Cell Line NCL20120045CR

Cholecystokinin CCKA/CCKAR Human Recombinant CCKA Receptor Stable Cell Line NCL20120046CR receptor CCKB/CCKBR Human Recombinant CCKB Stable Cell Line NCL20120047CR

GPR68/OGR1 Human Recombinant G-Protein Coupled Receptor 68 Stable Cell Line NCL20120048CR

Class A orphan Human Recombinant Mas-related G Protein Coupled Receptor Member MRGPRX2 NCL20120049CR X2 Stable Cell Line

Complement Human Recombinant Complement Component 5A Receptor 1 Stable Cell C5a/C5AR1 NCL20120050CR receptor Line

Human Recombinant Corticotropin Releasing Factor Receptor CRF1 CRF1/CRHR1 NCL20120051CR Corticotropin Stable Cell Line releasing factor Human Recombinant Corticotropin Releasing Factor Receptor CRF2 receptor CRF2 NCL20120052CR Stable Cell Line

(Continuing…)

09 02 GPCR Stable Cell Line Family Receptor Product Name Cat.No.

D2 Human Recombinant D2 Stable Cell Line NCL20120053CR

D5 Human Recombinant D5 Dopamine Receptor Stable Cell Line NCL20120054CR /DRD1 Human Recombinant D1 Dopamine Receptor Stable Cell Line NCL20120055CR

D2(Short) Human Recombinant D2 (Short) Dopamine Receptor Stable Cell Line NCL20120056CR

ETA/EDNRA Human Recombinant Receptor Type A Stable Cell Line NCL20120057CR ETB/EDNRB Human Recombinant Endothelin Receptor Type B Stable Cell Line NCL20120058CR

Formylpeptide FPR1 Human Recombinant Formylpeptide Receptor 1 Stable Cell Line NCL20120059CR receptor

Free fatty acid FFA2 Human Recombinant 2 Stable Cell Line NCL20120060CR receptor GPR40/FFA1 Human Recombinant Free Fatty Acid Receptor 1 Stable Cell Line NCL20120061CR

GALR1 Human Recombinant GAL1 Receptor Stable Cell Line NCL20120062CR GALR2 Human Recombinant GAL2 Galanin Receptor Stable Cell Line NCL20120063CR

Human Recombinant Releasing GHRHR NCL20120064CR Stable Cell Line

GLP1R Human Recombinant Glucagon-like Peptide 1 Receptor Stable Cell Line NCL20120065CR

Glucagon receptor GLP2R Human Recombinant Glucagon-like Peptide 2 Receptor Stable Cell Line NCL20120066CR

GCGR Human Recombinant Stable Cell Line NCL20120067CR

Human Recombinant Gastric Inhibitory Polypeptide Receptor Stable Cell GIPR NCL20120069CR Line

Gonadotropin Human Recombinant Gonadotropin-releasing Hormone Receptor Stable releasing hormone GNRHR NCL20120070CR Cell Line receptor

Growth hormone Human Recombinant Growth Hormone Secretagogue Receptor Stable releasing GHSR NCL20120071CR Cell Line receptor

H1/HRH1 Human Recombinant H1 Stable Cell Line NCL20120072CR

H2/HRH2 Human Recombinant H2 Histamine Receptor Stable Cell Line NCL20120073CR Histamine receptor H3/HRH3 Human Recombinant H3 Histamine Receptor Stable Cell Line NCL20120074CR

H4/HRH4 Human Recombinant H4 Histamine Receptor Stable Cell Line NCL20120075CR

(Continuing…)

10 02 GPCR Stable Cell Line Family Receptor Product Name Cat.No.

Hydroxycarboxylic GPR109A Human Recombinant Niacin Receptor 1 Stable Cell Line NCL20120076CR acid receptor

Kisspeptin receptor KiSS1/GPR54 Human Recombinant KiSS1-derived Peptide Receptor Stable Cell Line NCL20120077CR

Human Recombinant Like-1 Receptor Stable FPRL1 NCL20120078CR Cell Line CysLT1 Human Recombinant Cysteinyl Leukotriene Receptor 1 Stable Cell Line NCL20120079CR

LTB4R Human Recombinant Stable Cell Line NCL20120080CR

Lysophospholipid EDG4/LAP2 Human Recombinant EDG4 Lysophospholipid Receptor Stable Cell Line NCL20120081CR receptor

Human Recombinant Melanin-concentrating Hormone Receptor 1 Stable MCH1/GPR24 NCL20120082CR Cell Line Melanin concentrating Human Recombinant Melanin-concentrating Hormone Receptor 2 Stable hormone receptor MCH2/GPR145 NCL20120083CR Cell Line

MC2 Human Recombinant MC2 Stable Cell Line NCL20120084CR

MC3 Human Recombinant MC3 Melanocortin Receptor Stable Cell Line NCL20120085CR Melanocortin receptor MC4 Human Recombinant MC4 Melanocortin Receptor Stable Cell Line NCL20120086CR

MC5 Human Recombinant MC5 Melanocortin Receptor Stable Cell Line NCL20120087CR

MTNR1A Human Recombinant Melatonin MT1 Receptor Stable Cell Line NCL20120088CR MT2/MTNR1B Human Recombinant Melatonin MT2 Receptor Stable Cell Line NCL20120089CR

Metabotropic mGlu2/GRM2 Human Recombinant Metabotropic 2 Stable Cell Line NCL20120090CR glutamate receptor

Motilin receptor MTLR/MLNR Human Recombinant Receptor Stable Cell Line NCL20120091CR

NMU2 Human Recombinant Receptor 2 Stable Cell Line NCL20120092CR Neuromedin receptor NMU1/GPR66 Human Recombinant 1 Stable Cell Line NCL20120093CR

NPS1b Human Recombinant Receptor Isoform B Stable Cell Line NCL20120094CR NPSR1 Human Recombinant Neuropeptide S Receptor Isoform A Stable Cell Line NCL20120095CR

NPY2 Human Recombinant Receptor Y2 Stable Cell Line NCL20120096CR NPY4R Human Recombinant Neuropeptide Y Receptor Y4 Stable Cell Line NCL20120097CR

(Continuing…)

11 02 GPCR Stable Cell Line Family Receptor Product Name Cat.No.

Neurotensin receptor NTS1/NT1 Human Recombinant NTS1 Receptor Stable Cell Line NCL20120098CR

OPRD1 Human Recombinant δ- OPRD1 Stable Cell Line NCL20120099CR

OPRK1 Human Recombinant κ-Opioid Receptor OPRK1 Stable Cell Line NCL20120100CR Opioid & opioid-like receptor OPRM1 Human Recombinant μ-Opioid Receptor OPRM1 Stable Cell Line NCL20120101CR

OPRL1 Human Recombinant Opiate Receptor-like 1 Stable Cell Line NCL20120102CR

OX1 Human Recombinant OX1 Receptor Stable Cell Line NCL20120103CR OX2 Human Recombinant OX2 Orexin Receptor Stable Cell Line NCL20120104CR

P2Y receptor P2Y1 Human Recombinant P2Y1 Stable Cell Line NCL20120105CR

P3Y receptor P2Y2 Human Recombinant P2Y2 Purinergic Receptor Stable Cell Line NCL20120106CR

P4Y receptor P2Y4 Human Recombinant P2Y4 Purinergic Receptor Stable Cell Line NCL20120107CR

P5Y receptor P2Y6 Human Recombinant P2Y6 Purinergic Receptor Stable Cell Line NCL20120108CR

P6Y receptor P2Y11 Human Recombinant P2Y11 Purinergic Receptor Stable Cell Line NCL20120109CR

P7Y receptor Human Recombinant P2Y12 Purinergic Receptor Stable Cell Line NCL20120110CR

Parathyroid hormone PTH1/PTHR1 Human Recombinant Receptor 1 Stable Cell Line NCL20120111CR receptor

Peptide P518 receptor QRFP/GPR103 Human Recombinant G-Protein Coupled Receptor 103 Stable Cell Line NCL20120112CR

Platelet activating PAFR/PTAFR Human Recombinant Platelet Activating Factor Receptor Stable Cell Line NCL20120113CR factor receptor

Prolactin releasing Human Recombinant -releasing Peptide Receptor Stable Cell PRLHR NCL20120114CR peptide receptor Line

DP2 Human Recombinant Prostanoid Receptor DP2 Stable Cell Line NCL20120115CR

EP1/PTGER1 Human Recombinant EP1 Prostanoid Receptor Stable Cell Line NCL20120116CR

EP2/PTGER2 Human Recombinant EP2 Prostanoid Receptor Stable Cell Line NCL20120117CR

Prostanoid receptor FP/PTGFR Human Recombinant FP Prostanoid Receptor Stable Cell Line NCL20120118CR

IP1/PTGIR Human Recombinant Prostanoid IP1 Receptor Stable Cell Line NCL20120119CR

TP/TBXA2R Human Recombinant TP Prostanoid Receptor Stable Cell Line NCL20120120CR

PTGDR/DP1 Human Recombinant Prostanoid Receptor DP1 Stable Cell Line NCL20120121CR

(Continuing…)

12 02 GPCR Stable Cell Line Family Receptor Product Name Cat.No.

Prostanoid receptor DP2 Human Recombinant Prostanoid Receptor DP2 Stable Cell Line NCL20120115CR

Human Recombinant PAR2 Proteinase-activated Receptor Stable Cell PAR2 NCL20120122CR Line Proteinase-activated receptor Human Recombinant PAR4 Proteinase-activated Receptor Stable Cell PAR4/F2RL3 NCL20120123CR Line

SST1 Human Recombinant SST1 Stable Cell Line NCL20120124CR

SST2/SSTR2 Human Recombinant SST2 Somatostatin Receptor Stable Cell Line NCL20120125CR

Somatostatin receptor SST3 Human Recombinant SST3 Somatostatin Receptor Stable Cell Line NCL20120126CR

SST4/SSTR4 Human Recombinant SST4 Somatostatin Receptor Stable Cell Line NCL20120127CR

SST5/SSTR5 Human Recombinant SST5 Somatostatin Receptor Stable Cell Line NCL20120128CR

NK1/TACR1 Human Recombinant NK1 Stable Cell Line NCL20120129CR

Tachykinin receptor NK2/TACR2 Human Recombinant NK2 Tachykinin Receptor Stable Cell Line NCL20120130CR

NK3/TACR3 Human Recombinant NK3 Tachykinin Receptor Stable Cell Line NCL20120131CR

Thyrotropin releasing Human Recombinant TRH1 Thyrotropin-releasing Hormone Receptor TRH1/TRHR NCL20120132CR hormone receptor Stable Cell Line

Urotensin receptor UTR2 Human Recombinant Urotensin 2 Receptor Stable Cell Line NCL20120133CR

OT/OXTR Human Recombinant Receptor Stable Cell Line NCL20120134CR and V1B Human Recombinant V1B Stable Cell Line NCL20120135CR V2 Human Recombinant V2 Vasopressin Receptor Stable Cell Line NCL20120136CR

Human Recombinant Vasoactive Intestinal Polypeptide Receptor Stable PAC1 NCL20120137CR Cell Line VIP and PACAP receptor VPAC2 Human Recombinant VPAC2 Receptor Stable Cell Line NCL20120138CR

VPAC1/VIP1 Human Recombinant VPAC1 Receptor Stable Cell Line NCL20120139CR

Control HEK293/Gα15 Stable Cell Line NCL20120140CR

Control Human Recombinant Gα15 Stable Cell Line NCL20120141CR

Human Recombinant Gqi5 Stable Cell Line NCL20120142CR

13 02 GPCR Stable Cell Line Sensory neurons, also known as afferent neurons, are the nerve cells activated by sensory input from the environment.

Different types of sensory neurons have different sensory receptors that respond to different kinds of stimuli.

Function Type

Smell Olfactory Sensory Neuron

Taste Cell

Vision Photoreceptor Cell External Stimuli Auditory Hair Cell

Temperature Thermoreceptor Cell

Mechanical Force Mechanoreceptor Cell

Blood Glomus Cell Internal Stimuli Potentially Damaging Nociceptor Cell Stimuli

14 03 Sensory Neuron Culture Creative Biolabs' specialty media allows neuroscience researchers to drastically accelerate manufacture of neurons from human pluripotent stem cells.

NeuSenso™ hPSC NeuSenso™ Sensory NeuSenso™ Sensory Differentiation Medium Neurons Neurons Maturation Medium Human pluripotent stem cell Cryopreserved hiPSC- differentiation medium for the derived highly characterized For the maturation of rapid manufacture of dorsal sensory neurons Sensory Neurons into root ganglia-like sensory manufactured for off-the- electrophysiological neurons. shelf use. responsive neurons.

Fast. Generates sensory Consistent. Sensory Fast. Sensory Neurons neurons in an easy-to-use Neurons have a consistent need only one week of monolayer protocol in only lot-to-lot phenotype and maturation before firing seven days. post-thaw recovery. action potentials.

Efficient. Manufactures up Simple. Sensory Neurons Functional. Sensory to 12 million sensory are pure and maturation- Neurons possess potassium, neurons with 90% purity for ready with no need for calcium, and sodium BRN3A, ISL1, and mitomycin treatment. currents, including NaV 1.7. PERIPHERIN.

15 03 Sensory Neuron Culture Intracellular Ca2+, as one of the most important second messengers, is central to neuronal signaling. Abnormalities in Ca2+ signaling have severe pathological consequences and can result in neurodegeneration, disorders of the central nervous, etc.

Genetically encoded calcium indicators (GECIs) are widely used in the studies of neuron. The GECI binds to calcium ions and emits fluorescence visualizing intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes.

图片地址： https:///files/Articles/127www.fro ntiersin.org475/fphar HTML/image_m/fphar g001.jpg

Overview of GPCR Signal Detection by GCaMP Sensor [Reference: Partridge, J. G. (2015). Utilizing GCaMP transgenic mice to monitor endogenous Gq/11- coupled receptors. Frontiers in pharmacology, 6, 42.]

16 04 Calcium&Glutamate Signaling Glutamate is a nonessential amino acid, a major bioenergetic substrate for proliferating normal and neoplastic cells, and an excitatory neurotransmitter that is actively involved in biosynthetic, bioenergetic, metabolic, and oncogenic signaling pathways. Glutamate signaling activates a family of receptors consisting of metabotropic glutamate receptors (mGluRs) and ionotropic glutamate receptors (iGluRs), both of which have been implicated in chronic disabling brain disorders such as Schizophrenia and neurodegenerative diseases like Alzheimer's, Parkinson's, and multiple sclerosis.

GluR Signaling at the Synapse [Reference: Reiner, A., & Levitz, J. (2018). Glutamatergic Signaling in the Central Nervous System: Ionotropic and Metabotropic Receptors in Concert. Neuron, 98(6), 1080–1098. doi:10.1016/j.neuron.2018.05.018]

Genetically encoded fluorescent glutamate indicators (iGluSnFRs) enable neurotransmitter release and diffusion to be visualized in intact tissue.

17 04 Calcium&Glutamate Signaling Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR

Biosensors are genetically encoded fluorescent proteins (FP) attached to an additional protein sequence which makes them sensitive to small biomolecules (e.g., Ca2+). Adeno-associated virus (AAV) Biosensor products come as ready-to-use AAV virus. The viruses encode your chosen biosensor, either calcium or neurotransmitter sensors and are ready for in vivo injection.

Creative Biolabs offers a range of Calcium or Neurotransmitter Biosensor products that come ready-to-use, with a choice of promoter and the ability to include the Cre inducible (FLEX-ON) expression. We have packaged these indicators into the most commonly used AAV serotypes (AAV8 and AAV9).

Our Products Our collection of over 2,000 AAV products is made up of various genetic elements, packaged into different serotypes in multiple combinations. The table below shows the popular options available from us. Please contact us if you need a different serotype.

Available Options

ALB, aMHC, c-Fos, CAG, CaMKIIa, CK0.4, CK1.3, CMV, cTnT, EF1a, EFFS, GFAP, HCRApoE, MBP, Promoters MCK, MeCP2, NSE, PDX1, PGK, Rpe65, SST, Syn, TBG, UBC

Reporters GFP, mCherry, RFP, LacZ, Luciferase

Inducible reporters DIO-GFP, DIO-mCherry, DIO-RFP, DIO-LacZ

Inducible systems Cre, DIO, tTA

CRISPR/Cas9 Cas9, gRNA scaffold

Serotypes AAV1, AAV2, AAV5, AAV6, AAV7, AAV8, AAV9, AAV-DJ

Unsure which serotype to use? If you are unsure which serotype will work best with your application, try our AAV GFP Testing Kit (Cat. NEK-2101- ZP27). This kit contains CMV-GFP in AAV1, AAV2, AAV5. AAV6. AAV8, AAV9, and AAV-DJ serotypes so you can test the efficiency on different cell types.

18 04 Calcium&Glutamate Signaling Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR

1. Deciding which biosensor is most suitable for your research goal (calcium or glutamate， color, kinetics, SNR). 2. Considering whether you would like to include FLEX. 3. Choosing a promoter (universal promoter-CAG, or tissue specific neuron specific-synapsin promoter-Syn). 4. Choosing the AAV vector serotype (AAV8 or AAV9). Other serotypes are available upon request.

Biosensor Promoter Description AAV type Catalogue No.

iGluSnFR CMV AAV8-CMV-iGluSnFR AAV8 NTC2101010CR

Rapid detection of glutamate, improved Glutamate SNR; direct visualization of CMV AAV9-CMV-iGluSnFR AAV9 NTC2101011CR synaptic release (as opposed to Ca2+ imaging)

CaMPARI CAG AAV8-CAG-FLEX-CaMPARI AAV8 NTC2101001CR

Syn AAV8-Syn-CaMPARI AAV8 NTC2101013CR

Syn AAV8-Syn-FLEX-CaMPARI AAV8 NTC2101014CR High throughput calcium assays with CAG AAV9-CAG-FLEX-CaMPARI AAV9 NTC2101029CR cultured cells Syn AAV9-Syn-CaMPARI AAV9 NTC2101043CR Calcium Syn AAV9-Syn-FLEX-CaMPARI AAV9 NTC2101044CR

CAG AAV8-CAG-GCaMP3 AAV8 NTC2101009CR GCaMP3: Green indicator CAG AAV9-CAG-GCaMP3 AAV9 NTC2101041CR

Syn AAV8-pRSET.GCaMP5G(7.35) AAV8 NTC2101012CR GCaMP5: Green indicator CAG AAV9-pRSET.GCaMP5G(7.35) AAV9 NTC2101042CR

For more information, please visit our website: AAV Neural Tracers. (Continuing…)

19 04 Calcium&Glutamate Signaling Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR

Biosensor Promoter Description AAV type Catalogue No.

CAG AAV8-CAG-FLEX-GCaMP6s-WPRE AAV8 NTC2101004CR

Syn AAV8-Syn-FLEX-GCaMP6s-WPRE AAV8 NTC2101017CR GCaMP6s: Syn AAV8-Syn-GCaMP6s-WPRE AAV8 NTC2101024CR Improved SNR, slower kinetics; Green CAG AAV9-CAG-FLEX-GCaMP6s-WPRE AAV9 NTC2101032CR indicator Syn AAV9-Syn-FLEX-GCaMP6s-WPR AAV9 NTC2101047CR

Syn AAV9-Syn-GCaMP6s-WPRE AAV9 NTC2101058CR

CAG AAV8-CAG-FLEX-GCaMP6m-WPRE AAV8 NTC2101003CR

Syn AAV8-Syn-FLEX-GCaMP6m-WPRE AAV8 NTC2101016CR GCaMP6m: Syn AAV8-Syn-GCaMP6m-WPRE AAV8 NTC2101023CR Improved SNR, Calcium intermediate kinetics; Green indicator CAG AAV9-CAG-FLEX-GCaMP6m-WPRE AAV9 NTC2101031CR

Syn AAV9-Syn-FLEX-GCaMP6m-WPRE AAV9 NTC2101046CR

Syn AAV9-Syn-GCaMP6m-WPRE AAV9 NTC2101057CR

CAG AAV8-CAG-FLEX-GCaMP6f-WPRE AAV8 NTC2101002CR

Syn AAV8-Syn-FLEX-GCaMP6f-WPRE AAV8 NTC2101015CR GCaMP6f: Syn AAV8-Syn-GCaMP6f-WPRE AAV8 NTC2101022CR Improved SNR, faster kinetics; Green CAG AAV9-CAG-FLEX-GCaMP6f-WPRE AAV9 NTC2101030CR indicator Syn AAV9-Syn-FLEX-GCaMP6f-WPRE AAV9 NTC2101045CR

Syn AAV9-Syn-GCaMP6f-WPRE AAV9 NTC2101056CR

For more information, please visit our website: AAV Neural Tracers. (Continuing…)

20 04 Calcium&Glutamate Signaling Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR

Biosensor Promoter Description AAV type Catalogue No.

CAG AAV8-CAG-FLEX-NES-jRCaMP1a-WPRE AAV8 NTC2101005CR

Syn AAV8-Syn-FLEX-NES-jRCaMP1a-WPRE AAV8 NTC2101018CR

jRCaMP1a: Syn AAV8-Syn-NES-jRCaMP1a-WPRE AAV8 NTC2101025CR Red, improved SNR, slower kinetics, bright CAG AAV9-CAG-FLEX-NES-jRCaMP1a-WPRE AAV9 NTC2101037CR

Syn AAV9-Syn-FLEX-NES-jRCaMP1a-WPRE AAV9 NTC2101052CR

Syn AAV9-Syn-NES-jRCaMP1a-WPRE AAV9 NTC2101063CR

CAG AAV8-CAG-FLEX-NES-jRCaMP1b-WPRE AAV8 NTC2101006CR

Syn AAV8-Syn-FLEX-NES-jRCaMP1b-WPRE AAV8 NTC2101019CR jRCaMP1b: Syn AAV8-Syn-NES-jRCaMP1b-WPRE AAV8 NTC2101026CR Red, improved SNR, improved dynamic CAG AAV9-CAG-FLEX-NES-jRCaMP1b-WPRE AAV9 NTC2101038CR range, bright Syn AAV9-Syn-FLEX-NES-jRCaMP1b-WPRE AAV9 NTC2101053CR

Syn AAV9-Syn-NES-jRCaMP1b-WPRE AAV9 NTC2101064CR Calcium CAG AAV8-CAG-FLEX-NES-jRGECO1a-WPRE AAV8 NTC2101007CR

Syn AAV8-Syn-FLEX-NES-jRGECO1a-WPRE AAV8 NTC2101020CR

Syn AAV8-Syn-NES-jRGECO1a-WPRE AAV8 NTC2101027CR jRGECO1a: Red, improved SNR CAG AAV9-CAG-FLEX-NES-jRGECO1a-WPRE AAV9 NTC2101039CR

Syn AAV9-Syn-FLEX-NES-jRGECO1a-WPRE AAV9 NTC2101054CR

Syn AAV9-Syn-NES-jRGECO1a-WPRE AAV9 NTC2101065CR

CAG AAV8-CAG-FLEX-NES-jRGECO1b-WPRE AAV8 NTC2101008CR

Syn AAV8-Syn-FLEX-NES-jRGECO1b-WPRE AAV8 NTC2101021CR

Syn AAV8-Syn-NES-jRGECO1b-WPRE AAV8 NTC2101028CR jRGECO1b: Red, improved SNR CAG AAV9-CAG-FLEX-NES-jRGECO1b-WPRE AAV9 NTC2101040CR

Syn AAV9-Syn-FLEX-NES-jRGECO1b-WPRE AAV9 NTC2101055CR

Syn AAV9-Syn-NES-jRGECO1b-WPRE AAV9 NTC2101066CR

For more information, please visit our website: AAV Neural Tracers.

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